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Journal articles on the topic 'DNA hydrolysis'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Fekry, Mostafa I., and Kent S. Gates. "DNA-catalyzed hydrolysis of DNA phosphodiesters." Nature Chemical Biology 5, no. 10 (2009): 710–11. http://dx.doi.org/10.1038/nchembio.224.

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2

Zhou, Cong, Joshua L. Avins, Paul C. Klauser, Benjamin M. Brandsen, Yujeong Lee, and Scott K. Silverman. "DNA-Catalyzed Amide Hydrolysis." Journal of the American Chemical Society 138, no. 7 (2016): 2106–9. http://dx.doi.org/10.1021/jacs.5b12647.

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3

Franklin, Sonya J. "Lanthanide-mediated DNA hydrolysis." Current Opinion in Chemical Biology 5, no. 2 (2001): 201–8. http://dx.doi.org/10.1016/s1367-5931(00)00191-5.

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4

Chandra, Madhavaiah, Amit Sachdeva, and Scott K. Silverman. "DNA-catalyzed sequence-specific hydrolysis of DNA." Nature Chemical Biology 5, no. 10 (2009): 718–20. http://dx.doi.org/10.1038/nchembio.201.

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5

Unciuleac, Mihaela-Carmen, Aviv Meir, Chaoyou Xue, Garrett M. Warren, Eric C. Greene, and Stewart Shuman. "Clutch mechanism of chemomechanical coupling in a DNA resecting motor nuclease." Proceedings of the National Academy of Sciences 118, no. 11 (2021): e2023955118. http://dx.doi.org/10.1073/pnas.2023955118.

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Mycobacterial AdnAB is a heterodimeric helicase–nuclease that initiates homologous recombination by resecting DNA double-strand breaks (DSBs). The N-terminal motor domain of the AdnB subunit hydrolyzes ATP to drive rapid and processive 3′ to 5′ translocation of AdnAB on the tracking DNA strand. ATP hydrolysis is mechanically productive when oscillating protein domain motions synchronized with the ATPase cycle propel the DNA tracking strand forward by a single-nucleotide step, in what is thought to entail a pawl-and-ratchet–like fashion. By gauging the effects of alanine mutations of the 16 ami

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Singh, Reema, Sumudu R. Perera, George S. Katselis та ін. "A β-lactamase-producing plasmid from Neisseria gonorrhoeae carrying a unique 6 bp deletion in blaTEM-1 encoding a truncated 24 kDa TEM-1 penicillinase that hydrolyses ampicillin slowly". Journal of Antimicrobial Chemotherapy 74, № 10 (2019): 2904–12. http://dx.doi.org/10.1093/jac/dkz306.

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AbstractBackgroundSeven structurally related β-lactamase-producing plasmids have been characterized in penicillinase-producing Neisseria gonorrhoeae (PPNG) isolates. We characterized a variant (i.e. pJRD20, Canada type) of the Africa-type (pJD5) plasmid isolated from N. gonorrhoeae strain 8903.ObjectivesTo compare the DNA sequence of pJRD20 with that of pJD5 and pJD4 (Asia-type) and their TEM-1 β-lactamases.MethodsN. gonorrhoeae 8903 was identified as part of the Gonococcal Antimicrobial Surveillance Program in Canada. β-Lactamase production was assessed using nitrocefin. MICs were determined

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7

Zilhadia, Zilhadia, Afifah Nurul Izzah, and Ofa Suzanti Betha. "Perbandingan Metode SYBR Green dan Hydrolysis Probe dalam Analisis DNA Gelatin Sapi dan Gelatin Babi Menggunakan Real Time Polymerase Chain Reaction." Jurnal Sains Farmasi & Klinis 4, no. 1 (2017): 16. http://dx.doi.org/10.29208/jsfk.2017.4.1.194.

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Pemanfaatan gelatin secara luas menimbulkan kontroversi dan kekhawatiran bagi masyarakat muslim karena pada umumnya gelatin terbuat dari kulit babi dan sapi. Salah satu teknik analisis yang dapat membedakan gelatin sapi dan gelatin babi adalah Real Time Polymerase Chain Reaction (PCR). Real Time PCR merupakan metode analisis berbasis DNA yang handal, efektif, dan terpecaya. Dalam analisis kualitatif dan kuantitatif, Real Time PCR membutuhkan pewarna fluoresens. Pewarna fluoresens yang umum digunakan adalah SYBR green dan hydrolysis probe. Telah dilakukan perbandingan antara metode SYBR green d

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8

Jackson, A. P., and A. Maxwell. "Identifying the catalytic residue of the ATPase reaction of DNA gyrase." Proceedings of the National Academy of Sciences 90, no. 23 (1993): 11232–36. http://dx.doi.org/10.1073/pnas.90.23.11232.

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We propose a mechanism for the hydrolysis of ATP by the DNA gyrase B protein in which Glu42 acts as a general base and His38 has a role in aligning and polarizing the glutamate residue. We have tested this mechanism by site-directed mutagenesis, converting Glu42 to Ala, Asp, and Gln, and His38 to Ala. In the presence of wild-type A protein, B proteins bearing the mutations Ala42 and Gln42 show no detectable supercoiling or ATPase activities, while Asp42 and Ala38 proteins have reduced activities. In the DNA cleavage and relaxation reactions of gyrase, which do not require ATP hydrolysis, wild-

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9

Koehler, D. R., and P. C. Hanawalt. "Digestion of damaged DNA by the T7 DNA polymerase-exonuclease." Biochemical Journal 293, no. 2 (1993): 451–53. http://dx.doi.org/10.1042/bj2930451.

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We have investigated the 3′-5′-exonuclease activity of phage T7 DNA polymerase for its usefulness as an approach for the detection of lesions in DNA. Unlike the T4 DNA polymerase-exonuclease, which is commonly used to map the position and frequency of lesions in very small DNA fragments, T7 DNA polymerase-exonuclease is able to hydrolyse almost completely the large fragments from KpnI-restricted mammalian DNA. However, we found that the exonuclease was also able to hydrolyse DNA containing several kinds of lesions: cyclobutane pyrimidine dimers, thymine glycols, and mono-adducts of 4′-hydroxym

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10

Ott, R., and R. Krämer. "DNA hydrolysis by inorganic catalysts." Applied Microbiology and Biotechnology 52, no. 6 (1999): 761–67. http://dx.doi.org/10.1007/s002530051588.

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11

Klostermeier, Dagmar. "Single-molecule FRET reveals nucleotide-driven conformational changes in molecular machines and their link to RNA unwinding and DNA supercoiling." Biochemical Society Transactions 39, no. 2 (2011): 611–16. http://dx.doi.org/10.1042/bst0390611.

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Many complex cellular processes in the cell are catalysed at the expense of ATP hydrolysis. The enzymes involved bind and hydrolyse ATP and couple ATP hydrolysis to the catalysed process via cycles of nucleotide-driven conformational changes. In this review, I illustrate how smFRET (single-molecule fluorescence resonance energy transfer) can define the underlying conformational changes that drive ATP-dependent molecular machines. The first example is a DEAD-box helicase that alternates between two different conformations in its catalytic cycle during RNA unwinding, and the second is DNA gyrase

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12

Meunier, B. "Pseudo-hydrolysis of DNA involving metalloporphyrins." Journal of Inorganic Biochemistry 59, no. 2-3 (1995): 134. http://dx.doi.org/10.1016/0162-0134(95)97242-i.

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13

Dixon, Nicholas E., Rodney J. Geue, John N. Lambert, Shadi Moghaddas, Dierdre A. Pearce, and Alan M. Sargeson. "DNA hydrolysis by stable metal complexes." Chemical Communications, no. 11 (1996): 1287. http://dx.doi.org/10.1039/cc9960001287.

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14

Liao, T. H., and J. C. Hsieh. "Hydrolysis of p-nitrophenyl phenylphosphonate catalysed by bovine pancreatic deoxyribonuclease." Biochemical Journal 255, no. 3 (1988): 781–87. http://dx.doi.org/10.1042/bj2550781.

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The ability of bovine pancreatic DNAase to hydrolyse the synthetic substrate p-nitrophenyl phenylphosphonate (NPPP) is intrinsic and is not due to the contamination of the DNAase preparation by nonspecific phosphodiesterases because the activities of DNA and NPPP hydrolysis are co-eluted from a DEAE-cellulose column with use of the Ca2+-affinity elution method and because the two activities are decreased simultaneously when the purified enzyme is treated with Cu2+/iodoacetate, an active-site-labelling agent for DNAase. NPPP hydrolysis is facilitated by the metal ion-DNAase. At relatively high

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15

Ramreddy, T., Subhojit Sen, Basuthkar J. Rao, and G. Krishnamoorthy. "DNA Dynamics in RecA−DNA Filaments: ATP Hydrolysis-Related Flexibility in DNA." Biochemistry 42, no. 41 (2003): 12085–94. http://dx.doi.org/10.1021/bi034667k.

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16

Fattorini, Paolo, Giorgio Marrubini, Serena Bonin, et al. "Prolonged DNA hydrolysis in water: A study on DNA stability." Data in Brief 20 (October 2018): 1237–43. http://dx.doi.org/10.1016/j.dib.2018.08.120.

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17

BISWAS, Indranil, and Ravi VIJAYVARGIA. "Heteroduplex DNA and ATP induced conformational changes of a MutS mismatch repair protein from Thermus aquaticus." Biochemical Journal 347, no. 3 (2000): 881–86. http://dx.doi.org/10.1042/bj3470881.

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ATP hydrolysis by MutS homologues is required for the function of these proteins in mismatch repair. However, the function of ATP hydrolysis in the repair reaction is not very clear. We have examined the role of ATP hydrolysis in oligomerization of Thermus aquaticus (Taq) MutS protein in solution. Analytical gel filtration and cross-linking of MutS protein with disuccinimidyl suburate suggest that TaqMutS is a dimer in the presence of ATP. ATP binding and hydrolysis by TaqMutS reduces the heteroduplex-DNA binding by the protein. Using limited proteolysis we detected extensive conformational ch

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18

Shervington, Leroy A., and Oliver Ingham. "Investigating the Stability of Six Phenolic TMZ Ester Analogues, Incubated in the Presence of Porcine Liver Esterase and Monitored by HPLC." Molecules 27, no. 9 (2022): 2958. http://dx.doi.org/10.3390/molecules27092958.

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Previous published data from our group showed the encouraging in vitro activities of six phenolic temozolomide (TMZ) ester analogues (ES8–ES12 and ES14) with up to a five-fold increase in potency compared to TMZ against glioblastoma multiform cell lines and TMZ-resistant O6-methylguanine-DNA methyl transferase (MGMT)-positive primary cells. This study investigated the stabilities of the six phenolic TMZ ester analogues in the presence of porcine liver esterase (PLE) as a hydrolytic enzyme, using high-performance liquid chromatography (HPLC), monitored by a diode-array detector (DAD). Determini

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19

Maxwell, A., L. Costenaro, S. Mitelheiser, and A. D. Bates. "Coupling ATP hydrolysis to DNA strand passage in type IIA DNA topoisomerases." Biochemical Society Transactions 33, no. 6 (2005): 1460–64. http://dx.doi.org/10.1042/bst0331460.

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Type IIA topos (topoisomerases) catalyse topological conversions of DNA through the passage of one double strand through a transient break in another. In the case of the archetypal enzyme, DNA gyrase, it has always been apparent that the enzyme couples the free energy of ATP hydrolysis to the introduction of negative supercoiling, and the structural details of this process are now becoming clearer. The homologous type IIA enzymes such as topo IV and eukaryotic topo II also require ATP and it has more recently been shown that the energy of hydrolysis is coupled to a reduction of supercoiling or

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20

Cheng, Chien-Chung, Yan-Chen Huang, and Ming-Chan Liu. "DNA hydrolysis by tris-triazacyclononanes metal complexes." Journal of Inorganic Biochemistry 96, no. 1 (2003): 115. http://dx.doi.org/10.1016/s0162-0134(03)80586-x.

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21

Kajimura, Ayako, Jun Sumaoka, and Makoto Komiyama. "DNA hydrolysis by cerium(IV)–saccharide complexes." Carbohydrate Research 309, no. 4 (1998): 345–51. http://dx.doi.org/10.1016/s0008-6215(98)00158-x.

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22

Branum, Mark E., and L. Que. "Double-strand DNA hydrolysis by dilanthanide complexes." JBIC Journal of Biological Inorganic Chemistry 4, no. 5 (1999): 593–600. http://dx.doi.org/10.1007/s007750050382.

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23

de Roodt, Adolfo Rafael, Silvana Litwin, and Sergio O. Angel. "Hydrolysis of DNA by 17 snake venoms." Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 135, no. 4 (2003): 469–79. http://dx.doi.org/10.1016/s1532-0456(03)00169-8.

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24

Novikova, Tatiana S., Evgeny A. Ermakov, Elena V. Kostina, et al. "Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus." Current Issues in Molecular Biology 45, no. 12 (2023): 9887–903. http://dx.doi.org/10.3390/cimb45120617.

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Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain. In addition, DNA binding to a surface such as the cell membrane, can also affect its recognition by antibodies. Here, we analyzed the hydrolysis of short oligodeoxyribonucleotides (ODNs) immobilized on the microarray surface and in solution by catalytic anti-DNA antibodies from SLE pat

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25

Tang, Liang, Haiyan Zhao, Theodore Christensen, Zihan Lin, and Annie Lynn. "Visualizing ATP hydrolysis in a viral DNA-packaging molecular motor." Acta Crystallographica Section A Foundations and Advances 70, a1 (2014): C1604. http://dx.doi.org/10.1107/s2053273314083958.

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Many DNA viruses encode powerful molecular machines to package viral genome into preformed protein shells. These DNA-packaging motors contain an ATPase module that converts the chemical reaction of ATP hydrolysis to physical motion of DNA. We previously determined the structures of the DNA-packaging motor gp2 of Shigella phage Sf6 in the apo form and in complex with ADP and ATP-gamma-S (Zhao et al, 2013, PNAS, 110, 8075). Here we report the structure of gp2 in complex with its substrate ATP at 2.0 Angstrom resolution. To our knowledge, this is the first time to capture, at high resolution, a p

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26

Yamamoto, Yoji, Kazuyuki Miura, and Makoto Komiyama. "Site-selective hydrolysis of huge DNA by artificial restriction DNA cutter." Nucleic Acids Symposium Series 50, no. 1 (2006): 265–66. http://dx.doi.org/10.1093/nass/nrl132.

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27

Greenleaf, William B., Jingping Shen, Dahai Gai, and Xiaojiang S. Chen. "Systematic Study of the Functions for the Residues around the Nucleotide Pocket in Simian Virus 40 AAA+ Hexameric Helicase." Journal of Virology 82, no. 12 (2008): 6017–23. http://dx.doi.org/10.1128/jvi.00387-08.

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ABSTRACT The high-resolution structural data for simian virus 40 large-T-antigen helicase revealed a set of nine residues bound to ATP/ADP directly or indirectly. The functional role of each of these residues in ATP hydrolysis and also the helicase function of this AAA+ (ATPases associated with various cellular activities) molecular motor are unclear. Here, we report our mutational analysis of each of these residues to examine their functionality in oligomerization, DNA binding, ATP hydrolysis, and double-stranded DNA (dsDNA) unwinding. All mutants were capable of oligomerization in the presen

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28

McDougal, Vivien V., and Linda A. Guarino. "DNA and ATP Binding Activities of the Baculovirus DNA Helicase P143." Journal of Virology 75, no. 15 (2001): 7206–9. http://dx.doi.org/10.1128/jvi.75.15.7206-7209.2001.

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ABSTRACT P143 is a DNA helicase that tightly binds both double-stranded and single-stranded DNA. DNA-protein complexes rapidly dissociated in the presence of ATP and Mg2+. This finding suggests that ATP hydrolysis causes a conformational change in P143 which decreases affinity for DNA. This supports the model of an inchworm mechanism of DNA unwinding.

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29

Warren, Garrett M., Aviv Meir, Juncheng Wang, Dinshaw J. Patel, Eric C. Greene, and Stewart Shuman. "Structure–activity relationships at a nucleobase-stacking tryptophan required for chemomechanical coupling in the DNA resecting motor-nuclease AdnAB." Nucleic Acids Research 50, no. 2 (2021): 952–61. http://dx.doi.org/10.1093/nar/gkab1270.

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Abstract Mycobacterial AdnAB is a heterodimeric helicase-nuclease that initiates homologous recombination by resecting DNA double-strand breaks. The AdnB subunit hydrolyzes ATP to drive single-nucleotide steps of 3′-to-5′ translocation of AdnAB on the tracking DNA strand via a ratchet-like mechanism. Trp325 in AdnB motif III, which intercalates into the tracking strand and makes a π stack on a nucleobase 5′ of a flipped-out nucleoside, is the putative ratchet pawl without which ATP hydrolysis is mechanically futile. Here, we report that AdnAB mutants wherein Trp325 was replaced with phenylalan

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30

Szczelkun, Mark D. "Translocation, switching and gating: potential roles for ATP in long-range communication on DNA by Type III restriction endonucleases." Biochemical Society Transactions 39, no. 2 (2011): 589–94. http://dx.doi.org/10.1042/bst0390589.

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To cleave DNA, the Type III RM (restriction–modification) enzymes must communicate the relative orientation of two recognition sequences, which may be separated by many thousands of base pairs. This long-range interaction requires ATP hydrolysis by a helicase domain, and both active (DNA translocation) and passive (DNA sliding) modes of motion along DNA have been proposed. Potential roles for ATP binding and hydrolysis by the helicase domains are discussed, with a focus on bipartite ATPases that act as molecular switches.

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31

Xiao, Anshan, Jie Yang, Yuping Feng, Shengli Cao, and Yufen Zhao. "Hydrolysis of DNA by N-Phosphoryl Branched Peptide." Phosphorus, Sulfur, and Silicon and the Related Elements 180, no. 8 (2005): 1947–51. http://dx.doi.org/10.1080/104265090902705.

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32

Brandsen, Benjamin M., Anthony R. Hesser, Marissa A. Castner, Madhavaiah Chandra, and Scott K. Silverman. "DNA-Catalyzed Hydrolysis of Esters and Aromatic Amides." Journal of the American Chemical Society 135, no. 43 (2013): 16014–17. http://dx.doi.org/10.1021/ja4077233.

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33

Bjornson, Keith P., Dwayne J. Allen, and Paul Modrich. "Modulation of MutS ATP Hydrolysis by DNA Cofactors†." Biochemistry 39, no. 11 (2000): 3176–83. http://dx.doi.org/10.1021/bi992286u.

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34

Ripoll, Laurent, Philippe Reinert, Louis-Ferdinand Pépin, and Philippe H. Lagrange. "Interaction of macrolides with adornase during DNA hydrolysis." Journal of Antimicrobial Chemotherapy 37, no. 5 (1996): 987–91. http://dx.doi.org/10.1093/jac/37.5.987.

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35

SHEN, Hebai. "Specific hydrolysis of DNA by Ce4+-ODN hybrid." Science in China Series B 47, no. 1 (2004): 75. http://dx.doi.org/10.1360/02yb0169.

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36

Kitamura, Yoshihito, Jun Sumaoka, and Makoto Komiyama. "Hydrolysis of DNA by cerium(IV)/EDTA complex." Tetrahedron 59, no. 52 (2003): 10403–8. http://dx.doi.org/10.1016/j.tet.2003.06.005.

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37

Wan, Shu-Hui, Feng Liang, Xiao-Qin Xiong, et al. "DNA hydrolysis promoted by 1,7-dimethyl-1,4,7,10-tetraazacyclododecane." Bioorganic & Medicinal Chemistry Letters 16, no. 10 (2006): 2804–6. http://dx.doi.org/10.1016/j.bmcl.2006.01.106.

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38

Zhu, Xiao-fei, Min Fan, Xu-bin Wang, et al. "Hydrolysis of DNA by a Dipeptides Containing Histidine." International Journal of Peptide Research and Therapeutics 16, no. 4 (2010): 297–300. http://dx.doi.org/10.1007/s10989-010-9232-x.

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39

Falcone, Joseph M., and Harold C. Box. "Selective hydrolysis of damaged DNA by nuclease P1." Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology 1337, no. 2 (1997): 267–75. http://dx.doi.org/10.1016/s0167-4838(96)00172-0.

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40

Rodkey, L. S., G. G. Gololobov, C. A. Rumbley, et al. "DNA Hydrolysis by Monoclonal Autoantibody BV 04-01." Applied Biochemistry and Biotechnology 83, no. 1-3 (2000): 95–106. http://dx.doi.org/10.1385/abab:83:1-3:95.

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41

Costenaro, L., A. Maxwell, S. Mitelheiser, and A. D. Bates. "Coupling ATP hydrolysis to DNA strand passage in type IIA DNA topoisomerases." Biochemical Society Transactions 33, no. 6 (2005): 1460. http://dx.doi.org/10.1042/bst20051460.

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42

Lior, H., and A. Patel. "Improved toluidine blue-DNA agar for detection of DNA hydrolysis by campylobacters." Journal of Clinical Microbiology 25, no. 10 (1987): 2030–31. http://dx.doi.org/10.1128/jcm.25.10.2030-2031.1987.

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43

Nguyen, Hang Thi Thu, Young-Ju Jang, Sunjoo Jeong, and Jaehoon Yu. "DNA-specific autoantibody cleaves DNA by hydrolysis of phosphodiester and glycosidic bond." Biochemical and Biophysical Research Communications 311, no. 3 (2003): 767–73. http://dx.doi.org/10.1016/j.bbrc.2003.10.059.

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44

Pavlovic, Mirjana, Anna Kats, Michelle Cavallo, Ran Chen, James X. Hartmann, and Yehuda Shoenfeld. "Pathogenic and Epiphenomenal Anti-DNA Antibodies in SLE." Autoimmune Diseases 2010 (2010): 1–18. http://dx.doi.org/10.4061/2010/462841.

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The discoveries of natural and the development of manufactured highly efficient catalytic antibodies (abzymes) opens the door to many practical applications. One of the most fascinating is the use of such antibodies in human therapy and prevention (vaccination), of cancer, AIDS, autoimmune diseases. A special entity of naturally occurring DNA hydrolytic anti-DNA antibodies is emerging within past decades linked to autoimmune and lymphoproliferative disorders, such as systemic lupus erythematosus (SLE), multiple sclerosis (MS), Sjogren Syndrome (SS), B - Chronic lymphocytic leucosis (B-CLL), an

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45

Alvi, N. K., R. Y. Rizvi, and S. M. Hadi. "Interaction of quercetin with DNA." Bioscience Reports 6, no. 10 (1986): 861–68. http://dx.doi.org/10.1007/bf01116239.

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I n vitro experiments to study interaction of the mutagenic flavonoid quercetin with DNA are described. Calf thymus DNA treated with quercetin for various time periods was subjected to S1 nuclease hydrolysis. Thermal melting profles of treated DNA were also determined using St nuclease. The rate of DNA hydrolyzed after 1 hr of pre-treatment with quercetin was found to be only about 50% of that in its absence. However, after 10 and 24hrs of treatment with the drug, the rate of S1 nuclease hydrolysis was observed to be greater than that of native DNA. Thermal melting profiles of DNA, treated wit

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46

Cai, Shu Lan, Fa Mei Feng, Kang Quan Qiao, Ying Zhang, and Xiu Lan Zhang. "Hydrolytic Activity of a Neodymium(III) Complex in DNA Cleavage." Advanced Materials Research 900 (February 2014): 312–15. http://dx.doi.org/10.4028/www.scientific.net/amr.900.312.

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A azamacrocyclic compound with carboxyl branch, 5,5,7,12,12,1-hexamethy-1,4 ,8,11-tetraazacyclo- tetradecane-N/-acetic acid(L), and its neodymium complex ware synthesized and characterized. The mode of combination of the neodymium complex with DNA was investigated by UV-vis absorption spectroscopy methods. The cutting function of the neodymium complex to supercoiled DNA was studied by gel electrophoresis method. The results show that metal complex can bind to the phosphate of DNA double helix and promote the hydrolysis of phosphodiester bond of supercoiled DNA(Form I); Supercoiled form DNA was

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47

Soh, Young-Min, Iain Finley Davidson, Stefano Zamuner, et al. "Self-organization of parS centromeres by the ParB CTP hydrolase." Science 366, no. 6469 (2019): 1129–33. http://dx.doi.org/10.1126/science.aay3965.

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ParABS systems facilitate chromosome segregation and plasmid partitioning in bacteria and archaea. ParB protein binds centromeric parS DNA sequences and spreads to flanking DNA. We show that ParB is an enzyme that hydrolyzes cytidine triphosphate (CTP) to cytidine diphosphate (CDP). parS DNA stimulates cooperative CTP binding by ParB and CTP hydrolysis. A nucleotide cocrystal structure elucidates the catalytic center of the dimerization-dependent ParB CTPase. Single-molecule imaging and biochemical assays recapitulate features of ParB spreading from parS in the presence but not absence of CTP.

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48

Gilbert, H. J., and G. P. Hazlewood. "Studies on the structure and function cellulases and hemicellulases." Proceedings of the British Society of Animal Production (1972) 1993 (March 1993): 154. http://dx.doi.org/10.1017/s0308229600024788.

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Plant structural polysaccharides provide a major source of nutrient for ruminant livestock. These carbohydrates are not degraded by mammalian-derived enzymes, but are hydrolysed by rumen microbial plant cell wall hydrolases. In view of the pivotal role of microbial cellulases and xylanases in ruminant nutrition, there has been considerable interest in these enzymes. In this paper we wish to illustrate how recombinant DNA (rDNA) technology can be utilised to dissect the biochemistry and molecular architecture of these enzymes, and provides us with the opportunity of generating novel cellulases

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49

Cavallo, Michelle F., Anna M. Kats, Ran Chen, James X. Hartmann, and Mirjana Pavlovic. "A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus." Autoimmune Diseases 2012 (2012): 1–10. http://dx.doi.org/10.1155/2012/814048.

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Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the production of antibodies against a variety of self-antigens including nucleic acids. These antibodies are cytotoxic, catalytic (hydrolyzing DNA, RNA, and protein), and nephritogenic. Current methods for investigating catalytic activities of natural abzymes produced by individuals suffering from autoimmunity are mostly discontinuous and often employ hazardous reagents. Here we demonstrate the utility of dual-labeled, fluorogenic DNA hydrolysis probes in highly specific, sensitive, continuous, fluorescence-based mea

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Frolova, Natalya S., Nancy Schek, Nadia Tikhmyanova, and Thomas R. Coleman. "Xenopus Cdc6 Performs Separate Functions in Initiating DNA Replication." Molecular Biology of the Cell 13, no. 4 (2002): 1298–312. http://dx.doi.org/10.1091/mbc.01-08-0382.

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Abstract:

Cdc6 performs an essential role in the initiation of eukaryotic DNA replication by recruiting the minichromosome maintenance (MCM) complex onto DNA. Using immunodepletion/add-back experiments inXenopus egg extracts, we have determined that both Walker A (ATP binding) and Walker B (ATP hydrolysis) motifs ofXenopus Cdc6 (Xcdc6) are essential, but have distinct functional roles. Although Walker B mutant protein binds chromatin well, Walker A mutant protein binds chromatin poorly. Neither Walker A nor Walker B mutant protein, however, load appreciable MCM onto DNA. Herein, we provide evidence that

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