Relevant bibliographies by topics / DNA-linked inhibitor antibody assay

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  2. Dissertations / Theses
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Academic literature on the topic 'DNA-linked inhibitor antibody assay'

Author: Grafiati
Published: 4 June 2021
Last updated: 17 February 2022

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Journal articles on the topic "DNA-linked inhibitor antibody assay"

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Kožíšek, Milan, Václav Navrátil, Kateřina Rojíková, et al. "DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors." Biochemical Journal 475, no. 23 (2018): 3847–60. http://dx.doi.org/10.1042/bcj20180764.

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Influenza neuraminidase is responsible for the escape of new viral particles from the infected cell surface. Several neuraminidase inhibitors are used clinically to treat patients or stockpiled for emergencies. However, the increasing development of viral resistance against approved inhibitors has underscored the need for the development of new antivirals effective against resistant influenza strains. A facile, sensitive, and inexpensive screening method would help achieve this goal. Recently, we described a multiwell plate-based DNA-linked inhibitor antibody assay (DIANA). This highly sensiti
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Navrátil, Václav, Jiří Schimer, Jan Tykvart, et al. "DNA-linked Inhibitor Antibody Assay (DIANA) for sensitive and selective enzyme detection and inhibitor screening." Nucleic Acids Research 45, no. 2 (2016): e10-e10. http://dx.doi.org/10.1093/nar/gkw853.

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Tykvart, Jan, Václav Navrátil, Michael Kugler, et al. "Identification of Novel Carbonic Anhydrase IX Inhibitors Using High-Throughput Screening of Pooled Compound Libraries by DNA-Linked Inhibitor Antibody Assay (DIANA)." SLAS DISCOVERY: Advancing the Science of Drug Discovery 25, no. 9 (2020): 1026–37. http://dx.doi.org/10.1177/2472555220918836.

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The DNA-linked inhibitor antibody assay (DIANA) has been recently validated for ultrasensitive enzyme detection and for quantitative evaluation of enzyme inhibitor potency. Here we present its adaptation for high-throughput screening of human carbonic anhydrase IX (CAIX), a promising drug and diagnostic target. We tested DIANA’s performance by screening a unique compound collection of 2816 compounds consisting of lead-like small molecules synthesized at the Institute of Organic Chemistry and Biochemistry (IOCB) Prague (“IOCB library”). Additionally, to test the robustness of the assay and its
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Peerlinck, Kathelijne, Marc G. Jacquemin, Jef Arnout, et al. "Antifactor VIII Antibody Inhibiting Allogeneic but not Autologous Factor VIII in Patients With Mild Hemophilia A." Blood 93, no. 7 (1999): 2267–73. http://dx.doi.org/10.1182/blood.v93.7.2267.

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Abstract Two unrelated patients with the same Arg2150His mutation in the factor VIII (FVIII) C1 domain, a residual FVIII activity of 0.09 IU/mL, and inhibitor titres of 300 and 6 Bethesda Units, respectively, were studied. Further analysis of patient LE, with the highest inhibitor titer, showed that (1) plasma or polyclonal IgG antibodies prepared from LE plasma inhibited the activity of allogeneic (wild-type) but not of self FVIII; (2) the presence of von Willebrand factor (vWF) increased by over 10-fold the inhibitory activity on wild-type FVIII; (3) the kinetics of FVIII inhibition followed
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Peerlinck, Kathelijne, Marc G. Jacquemin, Jef Arnout, et al. "Antifactor VIII Antibody Inhibiting Allogeneic but not Autologous Factor VIII in Patients With Mild Hemophilia A." Blood 93, no. 7 (1999): 2267–73. http://dx.doi.org/10.1182/blood.v93.7.2267.407k21_2267_2273.

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Two unrelated patients with the same Arg2150His mutation in the factor VIII (FVIII) C1 domain, a residual FVIII activity of 0.09 IU/mL, and inhibitor titres of 300 and 6 Bethesda Units, respectively, were studied. Further analysis of patient LE, with the highest inhibitor titer, showed that (1) plasma or polyclonal IgG antibodies prepared from LE plasma inhibited the activity of allogeneic (wild-type) but not of self FVIII; (2) the presence of von Willebrand factor (vWF) increased by over 10-fold the inhibitory activity on wild-type FVIII; (3) the kinetics of FVIII inhibition followed a type I
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Gustafsdottir, Sigrun M., Stefan Wennström, Simon Fredriksson, et al. "Use of Proximity Ligation to Screen for Inhibitors of Interactions between Vascular Endothelial Growth Factor A and Its Receptors." Clinical Chemistry 54, no. 7 (2008): 1218–25. http://dx.doi.org/10.1373/clinchem.2007.099424.

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Abstract Background: Improved methods are required to screen drug candidates for their influences on protein interactions. There is also a compelling need for miniaturization of screening assays, with attendant reductions in reagent consumption and assay costs. Methods: We used sensitive, miniaturized proximity ligation assays (PLAs) to monitor binding of vascular endothelial growth factor A (VEGF-A) to 2 of its receptors, VEGFR-1 and VEGFR-2. We measured the effects of proteins and low molecular weight compounds capable of disrupting these interactions and compared the results with those obta
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Batsuli, Glaivy, Courtney Cox, John F. Healey, Pete Lollar, and Shannon L. Meeks. "Anti-Factor VIII C1 Domain Antibodies Are Present in the Plasmas of Patients with Hemophilia and Inhibitors." Blood 124, no. 21 (2014): 1482. http://dx.doi.org/10.1182/blood.v124.21.1482.1482.

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Abstract Hemophilia A is an X-linked disorder characterized by a deficiency or absence of blood coagulation protein factor VIII (fVIII). Treatment involves replacement of fVIII through infusions for acute bleeding episodes or prevention of bleeding events. Approximately 30% of individuals with severe hemophilia A will develop antibodies to fVIII. Many studies have characterized the antigenic properties of the C2 and A2 domains as these domains are considered the predominant immunogenic domains of the fVIII protein. However, there is increasing evidence that the C1 domain contributes to fVIII f
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David, Caroline A., Tim Middleton, Debra Montgomery та ін. "Microarray Compound Screening (μARCS) to Identify Inhibitors of HIV Integrase". Journal of Biomolecular Screening 7, № 3 (2002): 259–66. http://dx.doi.org/10.1177/108705710200700309.

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A novel high-throughput strand transfer assay has been developed, using Microarray Compound Screening (μARCS) technology, to identify inhibitors of human immunodeficiency virus (HIV) integrase. This technology utilizes agarose matrices to introduce a majority of the reagents throughout the assay. Integration of biotinylated donor DNA with fluorescein isothiocyanate (FITC)-labeled target DNA occurs on a SAM membrane in the presence of integrase. An anti-FITC antibody conjugated to alkaline phosphatase (AP) was used to do an enzyme-linked immunosorbent assay with the SAM. An agarose gel containi
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Trefz, G., B. Streit, C. W. E. Justus, W. Ebert, and M. D. Kramer. "Establishment of an Enzyme-Linked Immuno-Sorbent Assay for Urinary Trypsin Inhibitor by Using a Monoclonal Antibody." Journal of Immunoassay 12, no. 3 (1991): 347–69. http://dx.doi.org/10.1080/01971529108055077.

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Zhao, Jianan, Naijin Xu, and Hui Liu. "Quantitative Assessment of the Effects of Reducing Agents on Biological Macromolecules and on the Possible Repair of Oxidative Damage." BioMed Research International 2018 (August 2, 2018): 1–7. http://dx.doi.org/10.1155/2018/5704016.

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Objective. To quantitatively assess the influence of reducing agents on biological macromolecules and on the possible repair of oxidative damage. Methods. Samples (antibody, enzyme, DNA, and diluted serum) were treated with reducing agents (ammonium ferrous sulfate, ascorbic acid, potassium iodide, and sodium hyposulfite) in the experimental group and with NaCl in the control group. Enzyme-linked immunosorbent assay and quantitative PCR were used to determine the activity of antibody, enzyme, and DNA. Native gel electrophoresis (Native-PAGE) and sodium dodecyl sulfate polyacrylamide gel electr
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Dissertations / Theses on the topic "DNA-linked inhibitor antibody assay"

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Roberts, Anthony Simon. "The cloning, characterisation and engineering of an IGF-I-BINDING single chain Fv." Queensland University of Technology, 2004. http://eprints.qut.edu.au/15914/.

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This thesis describes the construction and characterisation of an insulin-like growth factor (IGF-I)-binding single chain Fv (scFv) and the utilisation of this scFv as a model protein for the study of the application of DNA shuffling and ribosome display to antibody engineering. The variable domain genes were isolated from the hybridoma cell line producing the monoclonal antibody and successfully joined by PCR for the construction of the scFv, named anti-GPE. Sequencing of the gene revealed an unusually short heavy chain CDR2 region. The cloned scFv was expressed in E. coli and purified. Ex
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Hejdánek, Jakub. "Identifikace sloučenin rozrušujících protein-proteinovou interakci u polymerasy viru chřipky." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-379358.

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Influenza virus causes severe respiratory infections in birds and mammals and it is responsible for up to half a million deaths of human beings worldwide each year. Two molecular targets in influenza viral life cycle, neuraminidase and M2 proton channel are exploited in treatment. However, the recent emergence of new pandemic type along with increasing resistance against approved drugs has urged the need for a new drug target discovery and potential search of its inhibitor. Recently, an interesting protein-protein interaction between two subunits PA and PB1 of influenza A viral polymerase has
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Conference papers on the topic "DNA-linked inhibitor antibody assay"

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Thompson, A. R. "ALLOANTIBODIES IN HEMOPHILIA B BINDING TO MULTIPLE FACTOR IX (IX) EPITOPES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644070.

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A high titer, boost responding inhibitor was present in a patient and his nephew, both with severe hemophilia B. On digests of their DNA, Southern blots hybridized to a cDNA were normal. They had no detectable IX antigen, including immuno-radiometric assays with a calcium-requiring polyclonal antibody fraction, in either serum or urine (less than 0.03 U/dl).Plasmas from the patient and his nephew had 12 and 25 NIH U/ml inhibitor titers, respectively. They were fractionated over IX-agarose with calcium. Unlike fractionation of rabbit polyclonal antibodies, EDTA eluates did not bind IX. Purified
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Asakura, S., N. Yoshida, and M. Matsuda. "MONOCLONAL ANTIBODIES AGAINST THROHBIN-ANTITHROMBIN III COMPLEX: EPITOPE SPECIFICITY AND EFFECT ON THROMBIN-ANTITHROMBIN III INTERACTION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643673.

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Among monoclonal antibodies (MCA´s) raised against human thrombin (T)-antithrombin m (AT) complex (TAT), two MCA´s designated as JITAT-16 and 17 with high affinity, Kd = 4.6nMand 4.1 nfi, respectively, were selected and characterized for specificity and functions. Their respective immunoglobulin subclasses are IgGi and IgG2a, and epitopes were found to be different from each Dther as shown by crisscross inhibition experiments. Immuno-alotting of normal plasma and serum electrophoresed on non-SDS aolyacrylamide gel showed that these antibodies reacted with normal serum but not with plasma. This
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Tanaka, I., A. Yoshioka, T. Fujiwara, H. Nakai, and H. Fukui. "THE CHANGES OF FACTOR VIII ANTIGEN DURING THE COAGULATION PROCESS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644038.

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The changes of factor VIII (F. VIII) during blood coagulation process is still controversial. We analyzed the F. VIII antigen (F. VIII:Ag) at various intervals of in vitro blood clotting by immunoassays using polyclonal and different kinds of monoclonal antibodies to F. VIII.We used two immunoassays, an immunoradiometric assay (IRMA) and an enzyme-linked immunosorbent assay (ELISA). The IRMA was performed by the method of Peake et al. using high-titer allo-antibodies to F. VIII. The ELISA was performed by two-site solid phase system consisting of alloantibodies as the first and one of three ki
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Haber, Edgar, Marchall T. Runge, Christoph Bode, Betsy Branscomb, and Janet Schnee. "ANTIBODY TARGETED FIBRINOLYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643723.

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Chemical conjugates of fibrin-specificantibodies and plasminogen activators. Urokinase or tPA were linked covalently toamonoclonal antibody specific for the amino terminus of the beta chain of human fibrin (59D8) by means of the unidirectionalcross-linking reagent SPDP. The fibrinolytic potency of the conjugates at equal amidolytic activities was compared to the native plasminogen activators in an assay measuring lysis of 1251-fibrin monomer covalently linked to Sepharose CL-4B. Urokinase was least potent, tPA exhibited a 10fold increase in fibrinolysis whereas both the urokinase and tPA antib
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Pancham, N., M. Dumas, J. Brown, T. C. Michaud, and W. J. Knowles. "SYNTHETIC PEPTIDE ANTIBODIES RECOGNIZE PLASMA AND RECOMBINANT FVIII." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644027.

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Monoclonal antibodies were raised against synthetic peptides corresponding to the N-termini of the 90kd and 80kd subunits of human FVIII. Preliminary screening was performed directly against the peptides (linked to albumin) coated onto polystyrene wells. IgG was purified by Protein A-Sepharose and affinity purified using the immunogen peptides linked to Sepharose. Immunoreactivity with both plasma and recombinant FVIII was compared by Western blotting, two-site ELISA employing a neutralizing rabbit anti-FVIII IgG as capture antibody, and inhibition in a fluid phase FVIII activity assay. None o
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Liao, Uland, Armando Tovar, Philip Felgner, and Abraham P. Lee. "A Microfluidic Approach and Enhancement Towards a Colorimetric Enzyme-Linked-Immunosorbant-Assay for Diagnostic Detection of Infectious Diseases." In ASME 2007 2nd Frontiers in Biomedical Devices Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/biomed2007-38105.

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Accounting for more than 13 million deaths a year, infectious diseases have become the world’s biggest killer of children and young adults worldwide [1]. Diagnostic tools and technologies are vital towards identifying the presence and treatment of these diseases. Detection methods have commonly relied on DNA using polymerase-chain-reaction (PCR), however antibody methods have become popular due to growing trends in technology and detection sensitivity. ImmPORT Therapeutics, a leading group in generating infectious disease proteome microarrays, has developed multiplex systems for comprehensive
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Christie, D. J., S. S. Lennon, and L. L. Wischnack. "QUININE CAN INHIBIT OR ENHANCE PRODUCTION OF MURINE ANTI-HUMAN PLATELET ANTIBODIES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644577.

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Quinine (Qn) can provoke potent antibodies capable of destroying platelets and inducing life-threatening immunologic thrombocytopenia (DITP). A question critical to understanding the mechanism of DITP is why do platelets from all normal individuals express Qn-induced antigens while only relatively few individuals make the destructive drug-dependent antibodies? This problem was investigated in a murine animal model. BALB/c mice (6-12wk) were injected intraperitoneally, every other week, with saline or lOOOpg of Qn (this dose of drug gave serum levels comparable to human therapeutic serum levels
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Fulcher, C. A., R. A. Houghten, S. de Graaf Mahoney, J. R. Roberts, and T. S. Zimmerman. "SYNTHETIC PEPTIDE PROBES OF FACTOR VIII IMMUNOLOGY AND FUNCTION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644768.

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In order to develop specific immunologic reagents for mapping functionally important sites on FVIII, we have prepared rabbit polyclonal antibodies against synthetic peptides of FVIII derived from regions along the entire FVIII amino acid sequence. To date, a total of 70 peptides have been synthesized and characterized by amino acid and HPLC analysis. The peptides were coupled to keyhole limpet hemocyanin with glutaraldehyde as a linkage reagent and used to immunize rabbits. Antisera were tested by ELISA assay on polystyrene microtiter plates coated with either the peptide immunogen, or purifie
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Tan, Xingzhi, Matthew Sung, and Frank Loganzo. "Abstract 1978: Antibody-drug conjugates with DNA inhibitor linker-payloads can overcome resistance in cultured tumor cells made resistant to conjugates with cleavable-linked auristatins." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-1978.

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Berndt, M. "STRUCTURE AND FUNCTION OF THE GLYCOPROTEIN Ib-IX COMPLEX." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643729.

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At high shear flow, the adhesion of platelets to the exposed vascular subendothelium requires von Willebrand factor (vWF) and is dependent upon a specific platelet membrane adhesion receptor, the human platelet membrane glycoprotein (GP) Ib-IX complex. Recent evidence suggests that vWFbinding to the GP Ib-IX complex plays an important role in other key aspects of hemostasis and thrombosis such as shear-induced platelet aggregation and the interaction of platelets with fibrin.Studies in our laboratory with a seriesof murine monoclonal antibodies directed against epitopes on GP lb, GP IX, or aga
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