Academic literature on the topic 'DNA methylation'

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Journal articles on the topic "DNA methylation"

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Dang, Pengtao, Xiao Wang, Haiqi Zhu, et al. "Abstract 5352: Targeting DNA methylation in T cells to improve the efficacy of immunotherapy." Cancer Research 83, no. 7_Supplement (2023): 5352. http://dx.doi.org/10.1158/1538-7445.am2023-5352.

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Abstract T-cells are critical mediators of immunity and immunologic memory. Their cell fates are regulated in part through epigenetic mechanisms, including DNA methylation. Recent genome-wide methylation analyses have revealed dynamic alterations in the methylome at various stages of development and differentiation of T cells. At single cell level, it is not easy to simultaneously collect RNA-seq and RBBS methylation profiling. An important task is to understand the expression change of which genes and pathways are regulated by DNA methylations, especially for the ones that are associated with
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KARAASLAN, Ezgi, Ceren ACAR, and Şükrü KARTALCI. "Şizofrenide Epigenetik Bakış Açısı: DNA Metilasyon Modelleri." Arşiv Kaynak Tarama Dergisi 31, no. 3 (2022): 204–12. http://dx.doi.org/10.17827/aktd.1096901.

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Schizophrenia is a mental disorder characterized by delusions, hallucinations and various behavioral disorders. Affecting approximately 1% of the world's population, schizophrenia not only affects patients, but also other members of the society. Genetic and environmental factors play roles in the etiology of the disorder.Genetics, neurodevelopmental disorder, drug use, urban life, alone or together can be counted as the factors that cause the disorder. Despite increasing studies in recent years, the factors causing the formation of schizophrenia have not been fully clarified and more research
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Mikaelsson, Mikael A., and Courtney A. Miller. "DNA methylation." Epigenetics 6, no. 5 (2011): 548–51. http://dx.doi.org/10.4161/epi.6.5.15679.

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Singal, Rakesh, and Gordon D. Ginder. "DNA Methylation." Blood 93, no. 12 (1999): 4059–70. http://dx.doi.org/10.1182/blood.v93.12.4059.

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Singal, Rakesh, and Gordon D. Ginder. "DNA Methylation." Blood 93, no. 12 (1999): 4059–70. http://dx.doi.org/10.1182/blood.v93.12.4059.412k40_4059_4070.

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Sermswan, R., S. Mongkolsuk, and S. Sirisinha. "Characterization of the Opisthorchis viverrini genome." Journal of Helminthology 65, no. 1 (1991): 51–54. http://dx.doi.org/10.1017/s0022149x00010439.

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ABSTRACTThe methylations of trematode genomic DNA were analyzed using restriction enzymes and Southern blot hybridization. Restriction enzymes MspI, HpaII, HhaI were used to probe CpG methylation while MboI, Sau3A, DpnI were used for A methylation. The results revealed that Opisthorchis viverrini, Fasciola gigantica and Gigantocotyle siamensis had neither CpG nor A methylations. The presence of highly repeated DNA elements was also demonstrated in O. viverrini genomic DNA.
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Vogelgsang, Lars, Azlan Nisar, Sebastian Alexander Scharf, et al. "Characterisation of Type II DNA Methyltransferases of Metamycoplasma hominis." Microorganisms 11, no. 6 (2023): 1591. http://dx.doi.org/10.3390/microorganisms11061591.

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Bacterial virulence, persistence and defence are affected by epigenetic modifications, including DNA methylation. Solitary DNA methyltransferases modulate a variety of cellular processes and influence bacterial virulence; as part of a restriction-modification (RM) system, they act as a primitive immune system in methylating the own DNA, while unmethylated foreign DNA is restricted. We identified a large family of type II DNA methyltransferases in Metamycoplasma hominis, comprising six solitary methyltransferases and four RM systems. Motif-specific 5mC and 6mA methylations were identified with
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Wang, Heng, Yumo Xie, Gaopo Xu, et al. "Abstract 5272: Aberrant DNA 5mC and 6mA methylations increase ACE2 expression in intestinal cancer cells susceptible to SARS-CoV-2 infection." Cancer Research 82, no. 12_Supplement (2022): 5272. http://dx.doi.org/10.1158/1538-7445.am2022-5272.

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Abstract Angiotensin converting enzyme II (ACE2) is the cellular receptor of SARS-CoV-2. At present, ACE2 receptor is considered to be the key component in the SARS-CoV-2 infection and transmitting in the host. Among the cancer patients with COVID-19, the gastrointestinal cancer is the second most prevalent. The MethyLight and QASM assays were used to evaluated the genomic DNA 5mC methylation, while the CviAII enzyme-based 6mA-RE-qPCR was applied to determine motif-specific DNA 6mA methylation. The 6mA and 5mC methylation analyses of the long interspersed nuclear elements 1 (LINE1) were used t
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Majchrzak-Celińska, A., M. Naskret-Barciszewska, M. Giel-Pietraszuk, W. Nowak, P. Śron, and A. Barciszewska. "P02.08.A The relations of focal and total DNA methylation in gliomas." Neuro-Oncology 24, Supplement_2 (2022): ii31. http://dx.doi.org/10.1093/neuonc/noac174.101.

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Abstract Background The role of epigenetic events in gliomagenesis is undoubtful. However, the role of specific pathological events is not so clear. It was shown that loss in total DNA methylation correlates with higher tumor malignancy and oxidative DNA damage. But promoter methylation of many genes was reported to be significant for gliomas’ malignancy and predictive for the treatment outcome. In carcinogenesis in general global DNA hypomethylation and focal hypermethylation coexist. The aim of our project was to evaluate the correlation between total DNA methylation and promoter methylation
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Huang, Yung-Hsin, Su Jianzhong, Yong Lei, et al. "DNA Epigenome Editing Using Crispr-Cas Suntag-Directed DNMT3A." Blood 128, no. 22 (2016): 2707. http://dx.doi.org/10.1182/blood.v128.22.2707.2707.

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Abstract DNA methylation, an epigenetic modification, has widespread effects on gene expression during development. However, our ability to assign specific function to regions of DNA methylation is limited by the poor correlation between global patterns of DNA methylation and gene expression. To overcome this barrier, we utilized nuclease-deactivated Cas9 protein fused to repetitive peptide epitopes (SunTag) recruiting multiple copies of antibody-fused de novo DNA methyltranferase 3A (DNMT3A) (CRISPR-Cas SunTag-directed DNMT3A) to amplify local DNMT3A concentration and to methylate genomic sit
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Dissertations / Theses on the topic "DNA methylation"

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Tasanasuwan, Piyama. "Targeted DNA methylation." Thesis, University of Sheffield, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.251476.

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MacLeod, A. Robert (Robert Alan) 1966. "DNA methylation and oncogenesis." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=39956.

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DNA methylation is a postreplicative covalent modification of the DNA which is catalysed by the DNA methyltransferase enzyme. DNA methylation plays an important role in controlling the gene expression profile of mammalian cells. The hypothesis presented in this thesis is that the expression of the DNA methyltransferase gene is upregulated by cellular oncogenic pathways, and that this induction of MeTase activity results in DNA hypermethylation and plays a causal role in cellular transformation. Novel DNA methyltransferase inhibitors may inhibit the excessive activity of DNA methyltransferase i
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Tavares, de Araujo Felipe. "DNA replication and methylation." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=37847.

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One of the main questions of modern biology is how our cells interpret our genetic and epigenetic information. DNA methylation is a covalent modification of the genome that is essential for mammalian development and plays an important role in the control of gene expression, genomic imprinting and X-chromosome inactivation (Bird and Wolffe, 1999; Szyf et al., 2000). Furthermore, changes in DNA methylation and DNA methyltransferase 1 (DNMT1) activity have been widely documented in a number of human cancers (Szyf, 1998a; Szyf et al., 2000).<br>In Escherichia coli, timing and frequency of initiati
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Tsusaka, Takeshi. "Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation." Kyoto University, 2018. http://hdl.handle.net/2433/232305.

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Wong, Nicholas Chau-Lun. "DNA methylation at the neocentromere /." Connect to thesis, 2006. http://eprints.unimelb.edu.au/archive/00001883.

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Carrió, Gaspar Elvira. "DNA Methylation Dynamics during Myogenesis." Doctoral thesis, Universitat de Barcelona, 2015. http://hdl.handle.net/10803/296312.

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Myogenesis is the differentiation process which encompasses the formation of skeletal muscle during development, regeneration and tissue homeostasis throughout life. Arising from embryonic or adult stem cells, the myogenic process comprehends the acquisition of a specialized cell identity and the loss of pluri/multipotent and proliferative capacities. Starting with the hypothesis that DNA methylation, together with other epigenetic mechanisms and the transcription factors, orchestrates the transcriptional program, this thesis provides a comprehensive picture of DNA methylation dynamics during
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Akman, Kemal. "Bioinformatics of DNA Methylation analysis." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-182873.

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陳桂儀 and Kwai-yi Jacqueline Chan. "DNA methylation and pediatric cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2002. http://hub.hku.hk/bib/B31970370.

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Ó, Riain Ciarán Liam. "DNA methylation in follicular lymphoma." Thesis, Queen Mary, University of London, 2010. http://qmro.qmul.ac.uk/xmlui/handle/123456789/1318.

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Follicular Lymphoma (FL) is a common B cell Non-Hodgkin Lymphoma with a median survival of 8-10 years. Patients frequently undergo transformation to a more aggressive lymphoma and this is associated with drastically reduced survival. The hallmark of FL is the t(14;18) translocation yet this alone is insufficient for lymphomagenesis. While a number of secondary genetic changes have been described, epigenetic studies have lagged behind. Epigenetics refers to mechanisms that alter gene expression without a change in the primary DNA sequence. DNA methylation was quantitatively profiled at 1505 CpG
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Gonçalves, Athanásio Camila. "DNA methylation in Daphnia magna." Thesis, University of Birmingham, 2016. http://etheses.bham.ac.uk//id/eprint/7140/.

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Daphnia magna is gaining interest as a model for epigenetic research. It is easy to maintain under laboratory conditions and has low genetic diversity due to parthenogenetic reproduction. The D. magna genome is responsive to a wide range of stimuli and genomics resources are being developed for this species. Despite these great advantages, information regarding the epigenome of D. magna and its regulation is still lacking. Thus, the main aim of this work was to describe the methylome of D.magna and investigate its regulation and responsiveness to environmentally relevant exposure conditions. D
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Books on the topic "DNA methylation"

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Jost, Jean-Pierre, and Hans-Peter Saluz, eds. DNA Methylation. Birkhäuser Basel, 1993. http://dx.doi.org/10.1007/978-3-0348-9118-9.

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Tost, Jörg, ed. DNA Methylation. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-522-0.

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Mills, Ken I., and Bernie H. Ramsahoye. DNA Methylation Protocols. Humana Press, 2002. http://dx.doi.org/10.1385/1592591825.

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Tost, Jörg, ed. DNA Methylation Protocols. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7481-8.

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I, Mills Ken, and Ramsahoye Bernie H, eds. DNA methylation protcols. Humana Press, 2002.

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B, Kobayashi Taku, ed. DNA methylation research trends. Nova Science Publishers, 2007.

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Vani︠u︡shin, B. F. DNA methylation in plants. Nova Science Publishers, Inc., 2008.

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Doerfler, Walter, and Petra Böhm, eds. DNA Methylation: Basic Mechanisms. Springer-Verlag, 2006. http://dx.doi.org/10.1007/3-540-31390-7.

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Jones, Peter A., and Peter K. Vogt, eds. DNA Methylation and Cancer. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-59696-4.

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H, Burdon R., ed. Molecular biology of DNA methylation. Springer-Verlag, 1985.

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Book chapters on the topic "DNA methylation"

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Jeltsch, Albert, and Renata Z. Jurkowska. "DNA Methylation." In The Chemical Biology of Nucleic Acids. John Wiley & Sons, Ltd, 2010. http://dx.doi.org/10.1002/9780470664001.ch14.

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Cucu, Natalia V. "DNA Methylation." In Nutrition in Epigenetics. Wiley-Blackwell, 2011. http://dx.doi.org/10.1002/9780470959824.ch2.

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Carlberg, Carsten, and Ferdinand Molnár. "DNA Methylation." In Human Epigenetics: How Science Works. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-22907-8_3.

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Frauer, Carina, Fabio Spada, and Heinrich Leonhardt. "DNA Methylation." In Genome Organization and Function in the Cell Nucleus. Wiley-VCH Verlag GmbH & Co. KGaA, 2011. http://dx.doi.org/10.1002/9783527639991.ch2.

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Upchurch Sweeney, C. Renn, J. Rick Turner, J. Rick Turner, et al. "DNA-Methylation." In Encyclopedia of Behavioral Medicine. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-1005-9_100500.

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Laird, Peter W. "DNA Methylation." In Development. Springer Berlin Heidelberg, 1999. http://dx.doi.org/10.1007/978-3-642-59828-9_24.

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Carlberg, Carsten, and Ferdinand Molnár. "DNA Methylation." In Human Epigenomics. Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-7614-5_4.

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Simola, Nicola, Micaela Morelli, Tooru Mizuno, et al. "DNA Methylation." In Encyclopedia of Psychopharmacology. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_1239.

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Zhang, Yan. "DNA Methylation." In Encyclopedia of Systems Biology. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_351.

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Carlberg, Carsten, Eunike Velleuer, and Ferdinand Molnár. "DNA Methylation." In Molecular Medicine. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-27133-5_6.

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Conference papers on the topic "DNA methylation"

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Diaz-Blanco, Ignacio, Jose M. Enguita-Gonzalez, Diego Garcia-Perez, Abel A. Cuadrado-Vega, Nuria Valdes-Gallego, and Maria Dolores Chiara-Romero. "Analysis of DNA methylation patterns in cancer samples using SOM." In ESANN 2024. Ciaco - i6doc.com, 2024. http://dx.doi.org/10.14428/esann/2024.es2024-42.

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Son, Joo-Hiuk. "Active Demethylation of Cancer Cells using Terahertz Radiation for Potential Cancer Treatment." In Conference on Lasers and Electro-Optics/Pacific Rim. Optica Publishing Group, 2022. http://dx.doi.org/10.1364/cleopr.2022.cmp3a_02.

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Carcinogenesis involves DNA methylation which is a primary alteration in DNA in the development of cancer before genetic mutation. Because the abnormal DNA methylation is found in most cancer cells, the assessment of DNA methylation using terahertz radiation can be a novel optical method to detect and control cancer. The methylation has been directly observed by terahertz time-domain spectroscopy and this epigenetic chemical change could be manipulated to the state of demethylation using resonant terahertz radiation. Demethylation of cancer cells is a key issue in epigenetic cancer therapy and
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"Unlocking DNA methylation with BSXplorer." In Биоинформатика регуляции и структуры геномов / системная биология. ИЦиГ СО РАН, 2024. http://dx.doi.org/10.18699/bgrs2024-1.2-25.

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Martyushova, V. G., A. M. Timofeeva, and S. E. Sedykh. "METHYLATION-DEPENDENT RESTRICTION ENDONUCLEASES FOR THE ANALYSIS OF METHYLATION IN PROMOTER REGIONS OF GENES ASSOCIATED WITH THE PATHOGENESIS OF ALZHEIMER’S SYNDROME." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-255.

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DNA methylation plays an important role in epigenetic inheritance. Methylation of promoter regions of genes associated with the pathogenesis of Alzheimer’s disease has been shown to change during the development of the disease. In this work, a methylation-dependent restriction endonuclease and qPCR were used to analyse this process.
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Hagestedt, Inken, Mathias Humbert, Pascal Berrang, et al. "Membership Inference Against DNA Methylation Databases." In 2020 IEEE European Symposium on Security and Privacy (EuroS&P). IEEE, 2020. http://dx.doi.org/10.1109/eurosp48549.2020.00039.

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Jing, Jian, Elizabeth Davidson, Brent Pedersen, Daniel LaFlamme, Ivana V. Yang, and David A. Schwartz. "DNA Methylation And Innate Immune Tolerance." In American Thoracic Society 2012 International Conference, May 18-23, 2012 • San Francisco, California. American Thoracic Society, 2012. http://dx.doi.org/10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a1367.

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Carvalho, GFS, LL Vieira, BM Wolff, et al. "EPIGENOMIC ANALYSIS REVEAL CRITICAL ASSOCIATION BETWEEN METHYLATION STATUS AND CLINICAL PHENOTYPE." In Resumos do 54º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2022. http://dx.doi.org/10.5327/1516-3180.140s1.5803.

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Objective: Copy number variations (CNVs) are DNA fragments deleted or duplicated in relation to a reference genome. Some genome variants, classified as variants with uncertain significance (VUS), do not present a safe conclusion with clinical phenotypes, which makes the diagnostic conclusion difficult. We know that hypermethylation of gene promoter regions often leads to transcriptional silencing, in addition to DNA methylation changes in gene and/or intergenic regions can play a critical role in genomic regulation and stability. With epigenomic investigation, we can gain a more comprehensive
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Almeida, V., SN Chehimi, GFS Carvalho, et al. "DIFFERENCE IN METHYLATION STATUS OF REMAINING ALLELE IN SIBLINGS WITH THE SAME DELETION SIZE IN 5P." In Resumos do 54º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2022. http://dx.doi.org/10.5327/1516-3180.140s1.6429.

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Objective: Cri Du Chat syndrome or 5psyndrome (OMIM #123450) is characterized by a genomic loss in the short arm of chromosome 5 and by variable clinical manifestations, that include high-pitched cry in newborns. The phenotypic variability in this syndrome may not be limited only to variations in gene structure – such as deletions, duplications, inversions, insertions and translocations – as DNA methylation mechanisms, which occurs mainly in the “CpG Islands”, are also possible. Therefore, we studied the methylation status of the remaining allele of region of breakpoint at 5pin siblings, an 11
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Okano, Larissa Miyuki, Alexandre Luiz Korte de Azevedo, Tamyres Mingorance Carvalho, Tathiane Maistro Malta, Mauro Antonio Alves Castro, and Luciane Regina Cavalli. "Characterization of an epigenetic regulatory network on basal-like breast cancer subtype and its impact on signaling pathways and biological processes." In Brazilian Breast Cancer Symposium 2024. Mastology, 2024. http://dx.doi.org/10.29289/259453942024v34s1029.

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Objective: The main objective of this study was to identify DNA methylation at the distal cis-regulatory genomic regions associated with the basal-like breast cancer (BLBC) subtype, construct an epigenetic regulatory network, and determine its impact on cancer-associated signaling pathways and biological processes. Methodology: BLBC (n=134) and non- -tumoral breast (n=84) samples with DNA methylation, mRNA, and miRNA expression data were downloaded from The Cancer Genome Atlas (TCGA) database using a pipeline of computational tools. DNA methylation patterns on cancer- -specific enhancers enric
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Zheng, Kai, Hanzhe Liu, Simin Zhu, Huamei Li, and Xiaozhou Chen. "DNA Methylation Analysis Methods for Cancer Research." In 5th International Conference on Information Engineering for Mechanics and Materials. Atlantis Press, 2015. http://dx.doi.org/10.2991/icimm-15.2015.188.

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Reports on the topic "DNA methylation"

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Muyle, Aline. Analysis of DNA Methylation. Instats Inc., 2024. http://dx.doi.org/10.61700/6ayq8hff26qxn1470.

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This three-day workshop provides comprehensive training in the analysis of DNA methylation, a key epigenetic modification influencing gene expression and controlling the spread of transposable elements in genomes. Led by Dr Aline Muyle, participants will gain theoretical knowledge and practical skills in data preprocessing, statistical analysis, and visualization using RStudio and various bioinformatic software using Bash scripts. The workshop is suited for researchers in Biostatistics, Biology, Genetics, Ecology, Evolution, Bioinformatics, Molecular Biology, and Medical Research.
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Head, Thomas J., and Susannah Gal. DNA Based Fluid Computing Using Methylation. Defense Technical Information Center, 2005. http://dx.doi.org/10.21236/ada436701.

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Yamamoto, Fumiichiro. DNA Methylation Alterations in Breast Cancer. Defense Technical Information Center, 2001. http://dx.doi.org/10.21236/ada405531.

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Yamamoto, Fumiichiro. DNA Methylation Alterations in Breast Cancer. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada408999.

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ไชยวงศ์คต, อาคม, ภาวพันธ์ ภัทรโกศล та สมชัย นิรุตติศาสน์. การตรวจหาภาวะการเปลี่ยนแปลงการควบคุมเหนือพันธุกรรมในตัวอย่างเซลล์ปากมดลูกที่มีความผิดปกติระดับต่างๆ : รายงานการวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2016. https://doi.org/10.58837/chula.res.2016.27.

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โรคมะเร็งปากมดลูกเป็นโรคมะเร็งที่พบมากเป็นอันดับสองในผู้หญิงทั่วโลก โดยเฉพาะในประเทศกำลังพัฒนา ซึ่งรวมประเทศไทยด้วย มีการศึกษาพบภาวะ global DNA hypomethylation ในเซลล์มะเร็งชนิดต่างๆ รวมถึงมะเร็งปากมดลูกด้วย การศึกษานี้จึงสนใจพัฒนาวิธี Enzyme linked immunosorbent assay (ELISA) ในการตรวจ global DNA methylation เพื่อตรวจกรองมะเร็งปากมดลูกซึ่งวิธี ELISA เหมาะสมกับประเทศกำลังพัฒนา วิธีดำเนินการวิจัย การศึกษานี้พัฒนาวิธี ELISA ในการตรวจ global DNA methylation พร้อมเปรียบเทียบผลกับวิธี bisulfite LINE1 pyrosequencing โดยใช้ตัวอย่างดีเอ็นเอที่สกัดจากเซลล์ปากมดลูกที่มีความผิดปกติระดับต่างๆ ผลการวิจัย โ
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Cullen, Kevin J. The Effect of DNA Methylation on IGF2 Expression. Defense Technical Information Center, 1998. http://dx.doi.org/10.21236/ada361324.

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Huang, Tim H. M. Epigenetic Changes in DNA methylation in Breast Cancer. Defense Technical Information Center, 1999. http://dx.doi.org/10.21236/ada384184.

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Repository, Science. Epigenetics – Blurring the Lines between Nature and Nurture. Science Repository OÜ, 2020. http://dx.doi.org/10.31487/sr.blog.12.

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Singal, Rakesh. Aberrant Promoter Methylation in Serium DNA as a Biomarker for Prostate Cancer. Defense Technical Information Center, 2005. http://dx.doi.org/10.21236/ada452528.

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Rusiecki, Jennifer A., Louis French, Zygmunt Galdzicki, et al. Epigenetic Patterns of TBI: DNA Methylation in Serum of OIF/OEF Servicemembers. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada585498.

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