Academic literature on the topic 'DNA origami'

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Journal articles on the topic "DNA origami"

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HALFORD, BETHANY. "DNA ORIGAMI." Chemical & Engineering News 84, no. 12 (March 20, 2006): 10. http://dx.doi.org/10.1021/cen-v084n012.p010.

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Laurel Oldach. "DNA origami inspired by paper origami." C&EN Global Enterprise 101, no. 23 (July 17, 2023): 5. http://dx.doi.org/10.1021/cen-10123-scicon4.

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Endo, Masayuki, and Hiroshi Sugiyama. "DNA Origami Nanomachines." Molecules 23, no. 7 (July 18, 2018): 1766. http://dx.doi.org/10.3390/molecules23071766.

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DNA can assemble various molecules and nanomaterials in a programmed fashion and is a powerful tool in the nanotechnology and biology research fields. DNA also allows the construction of desired nanoscale structures via the design of DNA sequences. Structural nanotechnology, especially DNA origami, is widely used to design and create functionalized nanostructures and devices. In addition, DNA molecular machines have been created and are operated by specific DNA strands and external stimuli to perform linear, rotational, and reciprocating movements. Furthermore, complicated molecular systems have been created on DNA nanostructures by arranging multiple molecules and molecular machines precisely to mimic biological systems. Currently, DNA nanomachines, such as molecular motors, are operated on DNA nanostructures. Dynamic DNA nanostructures that have a mechanically controllable system have also been developed. In this review, we describe recent research on new DNA nanomachines and nanosystems that were built on designed DNA nanostructures.
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Ribeiro, Yasmin de Araújo, Vitor Nolasco de Moraes, Danyel Fernandes Contiliani, and Tiago Campos Pereira. "Origami de DNA." Genética na Escola 15, no. 2 (May 24, 2020): 98–107. http://dx.doi.org/10.55838/1980-3540.ge.2020.349.

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O material genético carrega informações fundamentais para o desenvolvimento de todos os organismos, dos mais simples aos mais complexos. Durante muito tempo, o DNA foi visto apenas como o elemento detentor de informações essenciais para a célula. Contudo, recentemente, pesquisas inovadoras têm usado o DNA como uma molécula estrutural, como um tijolo molecular muito versátil. Nesse sentido, a tradicional e secular arte japonesa de dobrar o papel, o origami, inspirou uma nova nanotecnologia denominada origami de DNA. Nesta abordagem, os cientistas propõem dobraduras de DNA, formando diversas estruturas moleculares e abrindo portas para aplicações terapêuticas, na indústria de nanomateriais, no desenvolvimento da ciência básica e em muitas outras áreas.
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Andersen, Ebbe Sloth. "DNA origami rewired." Nature Nanotechnology 10, no. 9 (September 2015): 733–34. http://dx.doi.org/10.1038/nnano.2015.204.

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Choi, Charles Q. "Origami from DNA." Scientific American 294, no. 5 (May 2006): 28. http://dx.doi.org/10.1038/scientificamerican0506-28d.

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Bell, Nicholas, Silvia Hernandez-Ainsa, Christian Engst, Tim Liedl, and Ulrich Keyser. "DNA Origami Nanopores." Biophysical Journal 104, no. 2 (January 2013): 517a. http://dx.doi.org/10.1016/j.bpj.2012.11.2859.

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Bell, Nicholas A. W., Christian R. Engst, Marc Ablay, Giorgio Divitini, Caterina Ducati, Tim Liedl, and Ulrich F. Keyser. "DNA Origami Nanopores." Nano Letters 12, no. 1 (December 29, 2011): 512–17. http://dx.doi.org/10.1021/nl204098n.

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Zhang, Yiyang, Chao Wang, Yuanchen Dong, Dianming Wang, Tianyang Cao, Shuo Wang, and Dongsheng Liu. "Fold 2D Woven DNA Origami to Origami + Structures." Advanced Functional Materials 29, no. 22 (April 3, 2019): 1809097. http://dx.doi.org/10.1002/adfm.201809097.

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Wang, Shih-Ting, Melissa A. Gray, Sunting Xuan, Yiyang Lin, James Byrnes, Andy I. Nguyen, Nevena Todorova, et al. "DNA origami protection and molecular interfacing through engineered sequence-defined peptoids." Proceedings of the National Academy of Sciences 117, no. 12 (March 12, 2020): 6339–48. http://dx.doi.org/10.1073/pnas.1919749117.

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DNA nanotechnology has established approaches for designing programmable and precisely controlled nanoscale architectures through specific Watson−Crick base-pairing, molecular plasticity, and intermolecular connectivity. In particular, superior control over DNA origami structures could be beneficial for biomedical applications, including biosensing, in vivo imaging, and drug and gene delivery. However, protecting DNA origami structures in complex biological fluids while preserving their structural characteristics remains a major challenge for enabling these applications. Here, we developed a class of structurally well-defined peptoids to protect DNA origamis in ionic and bioactive conditions and systematically explored the effects of peptoid architecture and sequence dependency on DNA origami stability. The applicability of this approach for drug delivery, bioimaging, and cell targeting was also demonstrated. A series of peptoids (PE1–9) with two types of architectures, termed as “brush” and “block,” were built from positively charged monomers and neutral oligo-ethyleneoxy monomers, where certain designs were found to greatly enhance the stability of DNA origami. Through experimental and molecular dynamics studies, we demonstrated the role of sequence-dependent electrostatic interactions of peptoids with the DNA backbone. We showed that octahedral DNA origamis coated with peptoid (PE2) can be used as carriers for anticancer drug and protein, where the peptoid modulated the rate of drug release and prolonged protein stability against proteolytic hydrolysis. Finally, we synthesized two alkyne-modified peptoids (PE8 and PE9), conjugated with fluorophore and antibody, to make stable DNA origamis with imaging and cell-targeting capabilities. Our results demonstrate an approach toward functional and physiologically stable DNA origami for biomedical applications.
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Dissertations / Theses on the topic "DNA origami"

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Dunn, Katherine Elizabeth. "DNA origami assembly." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:dff1bafd-e355-4df5-968b-b0deb7e6f44f.

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This thesis describes my investigations into the principles underlying self-assembly of DNA origami nanostructures and discusses how these principles may be applied. To study the origami folding process I designed, synthesized and characterized a polymorphic tile, which could adopt various shapes. The distribution of tile shapes provided new insights into assembly. The origami tiles I studied were based on scaffolds derived from customized plasmids, which I prepared using recombinant DNA technology. I developed a technique to monitor incorporation of individual staples in real time using fluorescence, measuring small differences in staple binding temperatures (~0.5-5 °C). I examined the tiles using Atomic Force Microscopy and I found that a remarkably high proportion of polymorphic tiles folded well, which suggests that there are assembly pathways, arising from strong cooperation between staples. In order to analyse the tile shapes quantitatively, I developed a specialized image processing technique. For validation of the method, I generated and analysed simulated data, and the results confirmed that I could measure individual tile parameters with sub-pixel resolution. I studied eleven variants of the polymorphic tile, and I proved that minor staple modifications can be used to change the folding pathway dramatically. The strength of cooperation between staples affects their behaviour, which is also influenced by their length and base sequences. Paired staples are particularly significant in assembly, and there are clear parallels with protein folding. I describe in an Appendix how I applied origami assembly principles in the development of my concept for an autonomous rotary nanomotor utilizing the sequential opening of DNA hairpins (already used for linear motors). This device represents an advance over non-autonomous rotary motors and I have simulated its performance. In this thesis I have answered important questions about DNA origami assembly, and my findings could enable the development of more sophisticated DNA nanostructures for specific purposes.
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Seibert, Mark Marvin. "Protein Folding and DNA Origami." Doctoral thesis, Uppsala universitet, Molekylär biofysik, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-121549.

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In this thesis, the folding process of the de novo designed polypeptide chignolin was elucidated through atomic-scale Molecular Dynamics (MD) computer simulations. In a series of long timescale and replica exchange MD simulations, chignolin’s folding and unfolding was observed numerous times and the native state was identified from the computed Gibbs free-energy landscape. The rate of the self-assembly process was predicted from the replica exchange data through a novel algorithm and the structural fluctuations of an enzyme, lysozyme, were analyzed. DNA’s structural flexibility was investigated through experimental structure determination methods in the liquid and gas phase. DNA nanostructures could be maintained in a flat geometry when attached to an electrostatically charged, atomically flat surface and imaged in solution with an Atomic Force Microscope. Free in solution under otherwise identical conditions, the origami exhibited substantial compaction, as revealed by small angle X-ray scattering. This condensation was even more extensive in the gas phase. Protein folding is highly reproducible. It can rapidly lead to a stable state, which undergoes moderate fluctuations, at least for small structures. DNA maintains extensive structural flexibility, even when folded into large DNA origami. One may reflect upon the functional roles of proteins and DNA as a consequence of their atomic-level structural flexibility. DNA, biology’s information carrier, is very flexible and malleable, adopting to ever new conformations. Proteins, nature’s machines, faithfully adopt highly reproducible shapes to perform life’s functions robotically.
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Marras, Alexander Edison. "DNA Origami Mechanisms and Machines." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1366227349.

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Daljit, Singh Jasleen Kaur. "Lipid-interacting switchable DNA origami nanostructures." Thesis, The University of Sydney, 2022. https://hdl.handle.net/2123/28197.

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DNA nanotechnology allows for the programmable self-assembly of nanostructures of arbitrary shapes and sizes. DNA nanostructures can be hydrophobically modified for integration with lipid bilayers. Lipid-integrated DNA nanotechnology can allow for the study of membrane proteins and other fundamental biological processes. In this thesis, switchable lipid-interacting DNA origami nanostructures are introduced. First, dimeric DNA origami nanostructures are successfully programmed to monomerise upon switching. The design space of switchable DNA origami nanostructures is explored using molecular switching mechanisms such as strand displacement, ionic switching and pH switching, as well as photoswitching, an external switching mechanism. Following that, lipid-interacting DNA origami nanostructures are introduced. These include previously studied DNA origami tile and a novel DNA origami barrel nanopore. The DNA origami tile is decorated with cholesterols and its membrane binding is characterised. The optimal number of cholesterols for membrane binding is shown to be between four and eight cholesterols, and the optimal position of the cholesterols is at the edge of the tiles. The spacing between cholesterols and the tiles also affects membrane binding, with a larger spacing increasing membrane binding. Furthermore, these parameters are also shown to affect the aggregation of the cholesterol-modified tiles during folding, and hence the yield of correctly formed tiles. Reversible membrane binding of the tiles is demonstrated using a strand displacement mechanism. A toehold positioned proximal to the cholesterol group is found to decrease the efficiency of strand displacement for tiles not bound to a membrane. However, for membrane bound tiles, the toehold position does not affect strand displacement. Next, a novel switchable lipid-interacting DNA origami barrel nanopore (DOBN) is developed. The design of the DOBN is optimised to maximise the yield of the correctly folded structure. Following that, the switchability of the DOBN in response to strand displacement, pH switching and photoswitching is explored. Logic gates combining the different switching mechanisms are also developed and validated. Finally, selective membrane binding of the DOBN upon switching is successfully demonstrated. Ultimately, the findings in this thesis establish design guidelines for integrating complex switching mechanisms with membrane-binding DNA nanostructures. This paves the way for achieving dynamic control of complex membrane-interacting DNA nanostructures with potential applications in nanomedicine, biophysics, and nucleic acids research.
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Boemo, Michael Austin. "Computation by origami-templated DNA walkers." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:bdea667e-a9aa-484a-9db0-a816339e5594.

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Interactions between DNA molecules can be used to perform computation. These DNA computing systems often use DNA molecules as freely diusing reactants in a well-mixed solution. We demonstrate how DNA walkers tethered to an origami-templated track can perform computation. A DNA walker can block a track that intersects with its own, preventing another walker from stepping down this blocked track. These blockages are primitive operations that can be used to perform computation. This thesis demonstrates how blocking interactions between DNA walkers can evaluate formulae posed in propositional logic. When anchorages in the track are viewed as networked machines and the DNA walker is viewed as a coordinated message passed between them, DNA walker circuits can be modelled as a distributed system. Techniques from formal veri- cation can be used to check this system for errors, determining the probability with which the system will end up in a certain state. This forms the basis of a compiler that can automatically design a DNA walker circuit that evaluates a given propositional formula within a specied error tolerance. To show how DNA walker circuits can be simplied, we create a propositional logic system called blocking logic that is proven to be both sound and complete. DNA walker circuits can be implemented and measured experimentally by using fluorescence spectrophotometry to track the position of a walker on the track. To demonstrate proof of principle, circuits were built that implement NOT and NOR operators. To make these circuits operate with minimal error, dierent sources of possible error were investigated and quantied. Cumulatively, the novel contributions that this thesis makes to the eld are: • the experimental design and implementation of a DNA computing system that uses DNA walkers, • probabilistic model checking software that automatically designs these DNA walker circuits, • a propositional logic system that can simplify a DNA walker circuit to an equivalent circuit that uses fewer tracks.
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Hudoba, Michael W. "Force Sensing Applications of DNA Origami Nanodevices." The Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1471474143.

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Darcy, Michael Augusto. "High Force Applications of DNA Origami Devices." The Ohio State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1619092851712077.

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Geng, Yanli. "Metallization of DNA and DNA Origami Using a Pd Seeding Method." BYU ScholarsArchive, 2013. https://scholarsarchive.byu.edu/etd/3857.

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In this dissertation, I developed a Pd seeding method in association with electroless plating, to successfully metallize both lambda DNA and DNA origami templates on different surfaces. On mica surfaces, this method offered a fast, simple process, and the ability to obtain a relatively high yield of metallized DNA nanostructures. When using lambda DNA as the templates, I studied the effect of Pd(II) activation time on the seed height and density, and an optimal activation time between 10 and 30 min was obtained. Based on the Pd seeds formed on DNA, as well as a Pd electroless plating solution, continuous Pd nanowires that had an average diameter of ~28 nm were formed with good selectivity on lambda DNA. The selected Pd activation time was also applied to metallize "T"-shape DNA origami, and Au coated branched nanostructures with a length between 200-250 nm, and wire diameters of ~40 nm were also fabricated. In addition, I found that the addition of Mg2+ ion into the reducing agent and electroless plating solution could benefit the surface retention of Pd seeded DNA and Au plated DNA structures. This work indicated that DNA molecules were promising templates to fabricate metal nanostructures; moreover, the formation of Au metallized branched nanostructures showed progress towards nanodevice fabrication using DNA origami. Silicon surfaces were also used as the substrates for DNA metallization. More complex circular circuit DNA origami templates were used. To obtain high enough seed density, multiple Pd seeding steps were applied which showed good selectivity and the seeded DNA origami remained on the surface after seeding steps. I used distribution analysis of seed height to study the effect of seeding steps on both average height and the uniformity of the Pd seeds. Four-repeated palladium seedings were confirmed to be optimal by the AFM images, seed height distribution analysis, and Au electroless plating results. Both Au and Cu metallized circular circuit design DNA origami were successfully obtained with high yield and good selectivity. The structures were maintained well after metallization, and the average diameters of Au and Cu samples were ~32 nm and 40 nm, respectively. Electrical conductivity measurements were done on these Au and Cu samples, both of which showed ohmic behavior. This is the first work to demonstrate the conductivity of Cu metallized DNA templates. In addition, the resistivities were calculated based on the measured resistance and the size of the metallized structures. My work shows promising progress with metallized DNA and DNA origami templates. The resulting metal nanostructures may find use as conducting interconnects for nanoscale objects as well as in surface enhanced Raman scattering analysis.
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Marcus, Pierre. "Toward Scalable DNA algorithms." Electronic Thesis or Diss., Lyon, École normale supérieure, 2024. http://www.theses.fr/2024ENSL0024.

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Le domaine du calcul par ADN consiste à utiliser l'ADN comme un matériau dynamique. En interagissant ensemble, les brins d’ADN peuvent implémenter de petits algorithmes et effectivement calculer. Par exemple, l’état de l’art permet l’évaluation de circuits logiques, où les informations de l’évaluation des circuits sont encodées dans les reconfigurations d’assemblage de brins d'ADN. Un autre exemple d’approche consiste à attacher des brins d'ADN selon des règles définies, proches du Le domaine du calcul par ADN consiste à utiliser l'ADN comme un matériau dynamique. En interagissant ensemble, les brins d’ADN peuvent implémenter de petits algorithmes et effectivement calculer. Par exemple, l’état de l’art permet l’évaluation de circuits logiques, où les informations de l’évaluation des circuits sont encodées dans les reconfigurations d’assemblage de brins d'ADN. Un autre exemple d’approche consiste à attacher des brins d'ADN selon des règles définies, proches du concept de tuiles de Wang, sur des substrats constitués de grands objets fait en ADN, appelés origami d'ADN. Cependant, toutes les approches actuelles sont confrontées au défi du passage à l’échelle. Dans la plupart des designs, la taille de l'entrée du problème est liée, soit aux caractéristiques de l'origami d'ADN, soit au nombre de brins d'ADN mélangés dans l’expérience. Cependant, ce nombre de brins est limité à la fois d'un point de vue pratique, et aussi d'un point de vue théorique. En effet, le risque d’hybridation d’ADN non voulue augmente avec le nombre de brins. Dans cette thèse, nous voulons résoudre ce sujet de scalabilité, sur le problème particulier de la résolution de labyrinthes. Ce problème a déjà été résolu, mais de manière non réversible et non scalable. Nous proposons dans ce travail d'implémenter une marche aléatoire réversible sur un origami d'ADN. Notre objectif est double. Tout d'abord, nous concevons un design composé d’un nombre fixe de seulement quatre brins différents, quelle que soit la taille du labyrinthe. Ensuite, nous proposons l'utilisation de la réversibilité, qui est un facteur clé, car elle permet d'exploiter le hasard pour tenter de revenir en arrière pour effacer les erreurs d'hybridation. Dans la première partie, nous avons mené des expériences au cours desquelles nous avons fixé des chemins de manière statique sur un origami d'ADN que nous avons conçu. Nous validerons notre capacité à mener, observer et traiter ces expériences. Dans la seconde partie, nous proposons une implémentation d'une marche aléatoire réversible grâce à une variante de la technique de toehold exchange strand displacement. Nous avons mené et développé des expériences sur cette variante grâce à une approche bottom-up. Cette approche bottom-up expérimente d’abord en imitant la présence d’origami d’ADN grâce à des structures d’ADN plus petites. Puis dans un second temps en ajoutant la présence d’un origami d’ADN
The DNA computing field consists in using DNA as dynamic building blocks. By interacting together, they can implement small algorithms and effectively compute. Many successful approaches were made. For instance, by implementing logical circuits where reconfigurations of DNA complexes progressively evaluate the network. Another approach is to attach DNA strands according to defined rules to a substrate made of large DNA objects called DNA origami. However, all the current approaches face the challenge of scalability. In most designs, the size of the input is linked to either the DNA origami or the number of strands. The number of strands, is limited not only technically but also theoretically, as there is an inherent chance of hybridization error between two strands that are not fully complementary. In this thesis, we want to solve this scalability issue on the particular problem of maze solving. This problem was already solved in both in a non-reversible and non-scalable fashion. We propose to implement a reversible random walk walker on a DNA origami. Our point is twofold. First, we can make a design with only four different strands, no matter the size of the maze. Most importantly, using reversibility is a key factor, as it can harness randomness to reverse hybridization errors. In the first part, we conducted experiments where we attached static paths made of DNA strands on a DNA origami. We will validate our ability to both conduct, observe and process these experiments. In the second part, we propose an implementation of a reversible random walk using a variation of the toehold mediated strand displacement technique. We have conducted and developed experiments on this variation using a bottom-up approach. Our experiments led to preliminary results of the technique on a DNA origami
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Said, Hassan [Verfasser]. "Studien zu synthetischen DNA Origami-Strukturen / Hassan Said." München : Verlag Dr. Hut, 2016. http://d-nb.info/1094117692/34.

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Books on the topic "DNA origami"

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Valero, Julián, ed. DNA and RNA Origami. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3028-0.

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Yang, Yangyang. Artificially Controllable Nanodevices Constructed by DNA Origami Technology. Tokyo: Springer Japan, 2015. http://dx.doi.org/10.1007/978-4-431-55769-2.

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Blackburn, Ken. Samoloty origami: Modele i papiery do składania. Janki k. Warszawy: Agencja Wydawnicza Jerzy Mostowski, 2009.

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Meldolesi, Anna. La costola di Eva: Come l'antropologia molecolare ha rivoluzionato lo studio delle nostre origini. Catania: CUEN, 1999.

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Busacca, Helle. Diario epistolare a Corrado Pavolini. Edited by Serena Manfrida. Florence: Firenze University Press, 2014. http://dx.doi.org/10.36253/978-88-6655-583-4.

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Vulcano, 1964. Dopo un incontro a Cortona, la poetessa e scrittrice messinese Helle Busacca intraprende la stesura di un Diario epistolare dedicato a Corrado Pavolini, noto intellettuale e regista di origini toscane di cui è innamorata da più di vent’anni. Ciò a cui dà vita attraverso le pagine sinora inedite dedicate a una “storia senza storia” è un incandescente teatro del sé, in cui drammatizzazione dell’esperienza personale, elaborazione autorappresentativa ed espressione di un’interiorità viva e inquieta si fondono in un amalgama dai caratteri fortemente ibridi, veicolato da una dirompente forza comunicativa. Mentre l’interlocutore disperatamente inseguito si fa sempre più evanescente e irraggiungibile emerge inconfondibile, fra confessione e rivendicazione, la voce di una moderna eroina tragica.
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Bertelé, Matteo. Pavel Florenskij tra Icona e Avanguardia. Venice: Edizioni Ca' Foscari, 2019. http://dx.doi.org/10.30687/978-88-6969-350-2.

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La figura e il pensiero di Pavel Florenskij (1882-1937), il ‘Leonardo da Vinci russo’, godono da tempo di grande attenzione nel nostro Paese. Lo confermano gli atti del convegno internazionale promosso nel 2012 dal Centro Studi sulle Arti della Russia (CSAR) dell’Università Ca’ Foscari Venezia, quando studiosi provenienti da Italia, Russia, Stati Uniti e Gran Bretagna hanno ragionato sul fondamentale contributo offerto dal pensatore russo allo studio e alla valorizzazione della cultura figurativa russa dalle origini fino alla contemporaneità d’allora, con una particolare attenzione verso le sue forme espressive più genuine come l’Icona e l’Avanguardia storica. Il volume presenta le riflessioni critiche di quegli autorevoli studiosi, qui raccolte nelle rispettive lingue madri, all’insegna, secondo lo spirito della lezione florenskiana, di una stimolante intertestualità linguistica, disciplinare e metodologica.
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di Pagolo Morelli, Giovanni. Ricordi. Nuova edizione e introduzione storica. Edited by Claudia Tripodi. Florence: Firenze University Press, 2019. http://dx.doi.org/10.36253/978-88-6453-913-3.

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I Ricordi di Giovanni di Pagolo Morelli, da tempo noti agli storici, sono qui pubblicati per la prima volta in versione integrale e linguisticamente fedele al testo originale del manoscritto. A questa nuova edizione critica si accompagna un lungo saggio introduttivo sull’autore, sugli intenti della sua opera e sugli esiti che essa ebbe presso gli eredi cui era destinata. I 74 anni vissuti da Giovanni Morelli dagli anni ’70 del Trecento alla metà degli anni ’40 del secolo successivo attraversano un arco temporale decisivo per la storia fiorentina, segnato da una forte mobilità sociale di cui i Morelli, famiglia dalle origini modeste, furono protagonisti. La lettura dei Ricordi, unita all’analisi della corposa documentazione superstite a carico dell’autore e della sua casa, aiutano a comprendere la forte spinta all’ascesa sociale che animò il Morelli e le ragioni dell’affermazione dei suoi discendenti nel panorama delle famiglie più in vista del tardo Quattrocento a Firenze.
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Blackburn, Ken. Pocket flyers paper airplane book. New York: Workman Pub., 1998.

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Tabacco, Giovanni. La relazione fra i concetti di potere temporale e di potere spirituale nella tradizione cristiana fino al secolo XIV. Edited by Laura Gaffuri. Florence: Firenze University Press, 2011. http://dx.doi.org/10.36253/978-88-8453-995-3.

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«Date a Cesare quel che è di Cesare, a Dio ciò che è di Dio»: a partire dalla non facile interpretazione del celeberrimo passo del vangelo di Matteo (22,21), la monografia di Giovanni Tabacco qui riproposta percorre le tappe che definirono e plasmarono le relazioni tra potere civile e potere religioso in Occidente lungo tutto il millennio medievale. Pubblicato una prima volta nel 1950 dalla Facoltà di Lettere e Filosofia dell'Università degli Studi di Torino, lo studio del grande medievista torinese appartiene ai "classici" mai dimenticati della storiografia medievistica italiana. La monografia è preceduta da tre interventi introduttivi (Laura Gaffuri, Giovanni Miccoli, Gian Maria Varanini) dedicati al significato e all'attualità della riflessione di Giovanni Tabacco, e all'importante stagione di studi che, tra primo Novecento e immediato secondo dopoguerra, si interrogò sulle origini delle relazioni stato-chiesa.
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Endo, Masayuki, ed. DNA Origami. Wiley, 2022. http://dx.doi.org/10.1002/9781119682561.

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Book chapters on the topic "DNA origami"

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Ma, Zhipeng, Young-Joo Kim, Do-Nyun Kim, and Osamu Tabata. "DNA-DNA origami." In Encyclopedia of Polymeric Nanomaterials, 1–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-36199-9_321-1.

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Ma, Zhipeng, Young-Joo Kim, Do-Nyun Kim, and Osamu Tabata. "DNA-DNA Origami." In Encyclopedia of Polymeric Nanomaterials, 589–603. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-29648-2_321.

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Edwards, Angela, and Hao Yan. "DNA Origami." In Nucleic Acids and Molecular Biology, 93–133. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-38815-6_5.

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Liu, Huajie, and Chunhai Fan. "DNA Origami Nanostructures." In DNA Nanotechnology, 207–24. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-36077-0_10.

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Mueller, Jonathan Wolf. "DNA-Origami – DNA als faltbares Baumaterial." In essentials, 13–15. Wiesbaden: Springer Fachmedien Wiesbaden, 2022. http://dx.doi.org/10.1007/978-3-658-37770-0_4.

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Bastings, Maartje M. C. "Cellular Uptake of DNA Origami." In Methods in Molecular Biology, 209–29. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3028-0_13.

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Thomsen, Rasmus P., Rasmus S. Sørensen, and Jørgen Kjems. "Parallel Functionalization of DNA Origami." In Methods in Molecular Biology, 175–94. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3028-0_11.

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Ijäs, Heini, Mauri A. Kostiainen, and Veikko Linko. "Protein Coating of DNA Origami." In Methods in Molecular Biology, 195–207. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3028-0_12.

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Ramakrishnan, Saminathan, Guido Grundmeier, and Adrian Keller. "Directed Protein Adsorption Through DNA Origami Masks." In DNA Nanotechnology, 253–62. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8582-1_17.

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Dalmastri, Claudia, Weihua Han, Stefano Vespucci, Liqian Wang, and Piero Morales. "DNA Origami Structures Interfaced to Inorganic Nanodevices." In DNA Nanotechnology, 263–78. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8582-1_18.

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Conference papers on the topic "DNA origami"

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Weck, Johann M., Lea M. Wassermann, and Amelie Heuer-Jungemann. "DNA origami and DNA origami silica hybrids for biomedical applications." In Colloidal Nanoparticles for Biomedical Applications XVI, edited by Marek Osiński and Antonios G. Kanaras. SPIE, 2021. http://dx.doi.org/10.1117/12.2578358.

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Marmiesse, Marie, Raluca Tiron, Guillaume Thomas, Shimon Levi, and Xavier Baillin. "Nanoengineering DNA origami for lithography." In Novel Patterning Technologies for Semiconductors, MEMS/NEMS and MOEMS 2020, edited by Eric M. Panning and Martha I. Sanchez. SPIE, 2020. http://dx.doi.org/10.1117/12.2552064.

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Hann, Julia, Andreas Morschhauser, Andreas Heerwig, Jens Wolfram Erben, Danny Reuter, Valery Pavlov, Marc Lamy de la Chapelle, Michael Mertig, and Thomas Otto. "DNA origami for biosensor applications." In 2021 Smart Systems Integration (SSI). IEEE, 2021. http://dx.doi.org/10.1109/ssi52265.2021.9467014.

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Marras, Alex E., Haijun J. Su, and Carlos E. Castro. "Design of DNA Origami Machines and Mechanisms." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-87848.

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This research introduces DNA origami as a viable approach to design and fabricate nanoscale mechanisms and machines. DNA origami is a recently developed nanotechnology that has enabled the construction of objects with unprecedented nanoscale geometric complexity via self-assembly. These objects are made up of thousands of DNA base-pairs packed into 3D structures with typical dimensions of 10–100nm. The majority of DNA origami research to date focuses on assembly of static 2D or 3D structures. In this work, we aim to extend the scope of DNA origami to include design of objects with kinematically constrained moving parts. Borrowing concepts from macro-scale kinematic mechanisms, we propose the concept of DNA Origami Mechanisms and Machines (DOMM) comprised of multiple links connected by joints. The links are designed by bundling double stranded DNA (dsDNA) helices to achieve the desired geometry and stiffness. The joints are designed by combining links with strategic placement flexible single stranded DNA (ssDNA) to enable motion in specific degrees of freedom. We detail design approaches for links and common joints including revolute, prismatic, and spherical, and discuss their integration into higher order mechanisms. As a proof of concept, we built a nanoscale hinge (revolute joint) and integrated four of these hinges into a prototype DOMM, namely a Bennett 4-bar linkage, which can be completely folded into a closed bundle geometry and unfolded into an open square geometry with a specified kinematic motion path. A kinematic analysis shows that the DNA Bennett linkage closely follows the 3D motion path of the rigid body counterpart. Our results demonstrate that DNA origami has high potential for the design and assembly of nanoscale machines. The ultimate goal of this work is to develop a library of nanoscale DNA-based links and joints that can be widely used in the design and assembly of higher order mechanisms and machines. We anticipate that, in the future, these components can be used to build nanorobots for useful applications including drug delivery, nanomanufacturing, and biosensing.
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Koehler, Chase, Divita Mathur, Eric Henderson, and Robyn Lutz. "Probing the Security of DNA Origami." In 2018 IEEE International Symposium on Software Reliability Engineering Workshops (ISSREW). IEEE, 2018. http://dx.doi.org/10.1109/issrew.2018.00-14.

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Van Dongen, Jeanne E., Jan C. T. Eijkel, and Loes I. Segerink. "DNA-Origami Enabled Distance-Dependent Sensing." In 2022 IEEE Sensors. IEEE, 2022. http://dx.doi.org/10.1109/sensors52175.2022.9967092.

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Zhou, Lifeng, Alexander E. Marras, Carlos E. Castro, and Hai-jun Su. "Pseudo-Rigid-Body Models of Compliant DNA Origami Mechanisms." In ASME 2015 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/detc2015-46838.

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In this paper, we introduce the strategy of designing and analyzing compliant nanomechanisms fabricated with DNA origami which we call compliant DNA origami mechanism (CDOM). The rigid, compliant and flexible parts are constructed by a bunch of double-stranded DNA (dsDNA) helices, fewer dsDNA helices and single-stranded DNA (ssDNA) strands respectively. Just like in macroscopic compliant mechanisms, a CDOM generates its motion via deformation of at least one structural member. During the motion, strain energy is stored and released in the mechanism. These CDOM can suppress thermal fluctuations due to the internal mechanical energy barrier for motion. An example of compliant hinge joint and a bistable four-bar CDOM fabricated with DNA origami are discussed at the end of this paper. The classic pseudo-rigid-body (PRB) model for compliant mechanism is successfully employed to the analysis of these DNA origami nanomechanisms. This PRB model has been used to guide the design of a bistable CDOM for a desired energy landscape.
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Cui, Jianzhong, Zhixiang Yin, Jing Yang, and Xianya Geng. "Searching for Maximum Clique by DNA Origami." In 2018 14th International Conference on Natural Computation, Fuzzy Systems and Knowledge Discovery (ICNC-FSKD). IEEE, 2018. http://dx.doi.org/10.1109/fskd.2018.8687133.

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Huang, Jer-Shing. "DNA-origami assisted unidirectional single meta-emitters." In Fiber Lasers and Glass Photonics: Materials through Applications III, edited by Stefano Taccheo, Maurizio Ferrari, and Angela B. Seddon. SPIE, 2022. http://dx.doi.org/10.1117/12.2626723.

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Su, Hai-Jun, Carlos E. Castro, Alexander E. Marras, and Lifeng Zhou. "The Kinematic Principle for Designing DNA Origami Mechanisms: Challenges and Opportunities." In ASME 2015 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2015. http://dx.doi.org/10.1115/detc2015-46833.

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DNA origami nanotechnology is a recently developed self-assembly process for design and fabrication of complex 3D nanostructures using DNA as functional materials. This paper aims to review our recent progress in applying DNA origami to design of kinematic mechanisms of nanometer scale. These nanomechanisms, which we call DNA Origami Mechanisms (DOM), are made of relatively stiff bundles of double-stranded DNA (dsDNA) which function as rigid links, connected by highly compliant single-stranded DNA (ssDNA) strands which function as kinematic joints. The designs of kinematic joints such as revolute, prismatic, cylindrical, universal and spherical are presented. The steps as well as necessary software or experimental tools for designing DOM with DNA origami links and joints are detailed. To demonstrate the designs, we presented the designs of Bennett 4-bar and crank-slider linkages. At last, a list of technical challenges such as design automation, computational modeling are presented. These challenges could also be opportunities for mechanism and robotics community to apply the well developed kinematic theories and computational tools to design of nanorobots and nanomachines.
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Reports on the topic "DNA origami"

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Cândido, Ana, and Teresa Seabra. Processos de Classificação e de Categorização da Diversidade Cultural e Migratória das Populações no Contexto Europeu. Observatorio de Desigualdades, CIES-ISCTE, ISCTE – Instituto Universitário de Lisboa, June 2023. http://dx.doi.org/10.15847/ciesodwp012023.

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O debate em torno da classificação e da categorização da diversidade cultural e migratória das populações que vivem no espaço europeu tornou-se mais relevante e complexificou-se na presente década. Este debate é marcado por falta de consenso sobre que indicadores devem ser recolhidos para caracterizar a origem das populações. A falta de orientações específicas e as diferentes formas de classificação contribuem para uma heterogeneidade de categorias utilizadas para caracterizar a origem das populações, concebendo-se diferentes formas de ler e observar a realidade da diversidade cultural e migratória em cada país. Neste quadro, o principal objetivo deste documento de trabalho é proceder ao levantamento dos processos de classificação e de categorização da diversidade cultural e migratória das populações, em curso nos países europeus. O texto está organizado em três secções. Na primeira analisam-se os indicadores recolhidos nas estatísticas oficiais (censos) dos países europeus para classificar e categorizar a origem das populações. Na segunda alarga-se a análise aos modelos de classificação e de categorização das populações de origem imigrante utilizados na produção de conhecimento científico. Por último, considerando que todos estes processos analíticos transportam desafios, dilemas e problemas, sintetizam-se os principais problemas presentes nos esquemas de classificação e de categorização, organizados em quatro dimensões: (i) os problemas político-jurídicos; (ii) os problemas de reducionismo; (iii) os problemas de comparabilidade; e (iv) o problema da exterioridade.
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da Silva, Anderson, and Ítalo Souza. Mapa interativo da capacidade de suporte de carga de solos da Bacia Hidrográfica do Rio Preto - BA. Instituto Federal Goiano, October 2020. http://dx.doi.org/10.33837/cr.map.0120.

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Este produto foi construído sob a ótica de conservação dos solos do Cerrado, em especial no que tange a manutenção de sua qualidade estrutural de acordo com o tipo de uso e cobertura. A origem da produção é a dissertação de mestrado defendida em 2020 no PPG-CRENAC intitulada “Sensoriamento remoto temporal da capacidade de suporte de carga de solos sob diferentes tipos de uso, cobertura e declividade na Bacia do Rio Preto, Oeste Baiano”. Com este mapa interativo é possível obter, com precisão razoável, estimativas da capacidade de suporte de carga dos solos da Bacia sem a necessidade de incursões a campo, em áreas de uso agrícola ou vegetação nativa.
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Cavalcante, Krisdany Vinícius S. M., and Ranny L. X. N. Michalski. Sobrac e SPA assinam acordo de cooperação mútua: Brasil e Portugal se alinham em prol da acústica! Revista Acústica e Vibrações, December 2021. http://dx.doi.org/10.55753/aev.v36e53.59.

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O artigo descreve os objetivos do acordo de cooperação técnico-científica firmado entre a Sociedade Brasileira de Acústica (Sobrac) e a Sociedade Portuguesa de Acústica (SPA) e registra sua origem e motivação. A experiência de representantes da língua portuguesa em um projeto de pesquisa internacional demonstrou a importância de atuarem conjuntamente, nessa e em futuras oportunidades, para o desenvolvimento da acústica no Brasil, em Portugal e no mundo.
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Varsano, Ricardo. A tributação do valor adicionado, o ICMS e as reformas necessárias para conformá-lo às melhores práticas internacionais. Inter-American Development Bank, February 2014. http://dx.doi.org/10.18235/0007889.

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O Brasil introduziu o imposto sobre o valor adicionado (IVA) em nível estadual em 1967, o ICM, rebatizado em 1988 de ICMS - numa época em que a base das poucos IVAs existentes não incluía a prestação de serviços. Foi então, e continuou a ser até os anos 1990 o único IVA subnacional no mundo. Atualmente, muitos analistas tributários consideram o ICMS um bom exemplo do que um IVA não deve ser. Várias de suas deficiências remontam ao tempo em que o ICM foi concebido, mas muitos outros foram adicionados ao longo do tempo na medida em que os governos estaduais concederam inúmeras isenções e, ao mesmo tempo, tentaram aumentar a receita por meio de medidas para facilitar a cobrança do imposto no curto prazo, mas que têm efeitos deletérios sobre a economia e, consequentemente, sobre a receita futura. As tentativas para melhorar a qualidade do ICM e do ICMS por meio de reformas, em sua maioria falharam. Este trabalho, depois de discutir a natureza, origem e a difusão do IVA pelo mundo, compara as características do ICMS com as de um IVA ideal, e com a de muitos dos IVAs atualmente em vigor no mundo, com especial atenção aos que financiam governos subnacionais. O objetivo é proporcionar base de informação para o debate sobre uma reforma global do ICMS, de preferência no âmbito da reforma geral da tributação do consumo e das relações federativas no Brasil. As seções finais discutem as reformas necessárias para aproximar as características do ICMS àquelas consideradas na literatura sobre o IVA como as melhores práticas, e sugerir um método para a realização do processo de reforma.
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Souza, Carlota Rocha de Matos, and Cibele Isaac Saad Rodrigues. Manual ilustrado de boas práticas para acessos vasculares para hemodiálise. Pontifícia Universidade Católica de São Paulo. Faculdade de Ciências Médicas e da Saúde, December 2023. http://dx.doi.org/10.23925/ripucsp/40716.

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Foi elaborado um guia de boas práticas para manipulação de acessos vasculares para hemodiálise ilustrado, que está contido na dissertação de mestrado como Anexo, intitulado: “Guia de boas práticas para manipulação de acessos vasculares de hemodiálise desenvolvido como material educativo para as clínicas participantes”. Esse manual ensina o passo a passo e os cuidados para manipulação de acessos vasculares para hemodiálise, cateter venoso central e fístula arteriovenosa (CVC e FAV). Esse mesmo estudo que deu origem ao manual pode ter continuidade e ser empregado como base para futuras análises de outros procedimentos utilizados em clínicas de hemodiálise e contribuir, dessa forma, para o conhecimento na área de enfermagem em nefrologia, que ainda carece de estudos, especialmente envolvendo a equipe técnica de enfermagem em unidades de hemodiálise, local de alta complexidade e de risco para a ocorrência de eventos adversos, mas também de melhorias substantivas da assistência. Foram aplicados três instrumentos de coleta de dados: o primeiro para traçar o perfil sociodemográfico dos participantes no primeiro encontro. O segundo, elaborado pelas pesquisadoras e validado por nove juízes, e foi baseado no inquérito KAP (Knowledge, Attitudes and Practice), que foi aplicado pré e pós-intervenção imediata e tardia (3 meses após o término) e o terceiro constituiu-se em pesquisa de satisfação utilizando o Net Promoter Score. Todos esses instrumentos podem ser utilizados em trabalhos semelhantes de capacitação interprofissional em TEA.
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