Log in

Relevant bibliographies by topics / DNA probe assay

Contents

Journal articles
Dissertations / Theses
Books
Book chapters
Conference papers

Academic literature on the topic 'DNA probe assay'

Author: Grafiati

Published: 4 June 2021

Last updated: 2 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'DNA probe assay.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "DNA probe assay"

1

Carpenter, W. R., T. E. Schutzbank, V. J. Tevere, K. R. Tocyloski, N. Dattagupta, and K. K. Yeung. "A transcriptionally amplified DNA probe assay with ligatable probes and immunochemical detection." Clinical Chemistry 39, no. 9 (1993): 1934–38. http://dx.doi.org/10.1093/clinchem/39.9.1934.

Full text

Abstract:

Abstract Transcriptionally amplified DNA probes are valuable tools in the development of sensitive nucleic acid-based diagnostic assays. Here we describe a model assay using a novel oligonucleotide hairpin probe that encodes a T7 RNA polymerase promoter. The hairpin probe and an adjacently hybridizing biotinylated capture probe were hybridized to target DNA and the duplex was captured onto streptavidin-coated magnetic particles. After ligation of the immobilized probes, which served to maintain specificity, the hairpin probe was transcribed by T7 RNA polymerase. The amplified RNA product was h

APA, Harvard, Vancouver, ISO, and other styles

2

Arnold, L. J., P. W. Hammond, W. A. Wiese, and N. C. Nelson. "Assay formats involving acridinium-ester-labeled DNA probes." Clinical Chemistry 35, no. 8 (1989): 1588–94. http://dx.doi.org/10.1093/clinchem/35.8.1588.

Full text

Abstract:

Abstract We describe the development of several hybridization assay formats involving acridinium-ester-labeled DNA probes. The simplest of these is a homogeneous assay procedure that requires only three steps to complete, including a 5-s detection step. Using this format, we have detected target sequences in the 10(-16) to 10(-17) mol range; when rRNA is the target, this translates to 3000 to 300 bacterial organisms. The entire assay can be carried out in less than 30 min. This is the first homogeneous DNA probe assay to be of practical use in the clinical laboratory, and it represents a major

APA, Harvard, Vancouver, ISO, and other styles

3

Chan, S. K., Y. S. Choong, D. Perera, and T. S. Lim. "Dengue serotyping with a label-free DNA sensor." Analytical Methods 10, no. 2 (2018): 214–22. http://dx.doi.org/10.1039/c7ay02131c.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Nitsche, Andreas, Nina Steuer, Christian Andreas Schmidt, Olfert Landt, and Wolfgang Siegert. "Different Real-Time PCR Formats Compared for the Quantitative Detection of Human Cytomegalovirus DNA." Clinical Chemistry 45, no. 11 (1999): 1932–37. http://dx.doi.org/10.1093/clinchem/45.11.1932.

Full text

Abstract:

Abstract Background: The aim of this study was to compare the ABI PRISM 7700 Sequence Detection System and the LightCycler to develop a quantitative real-time PCR assay for the detection of human cytomegalovirus (HCMV) DNA suitable for routine hospital application. Methods: We used one exonuclease probe and five different hybridization probe sets as sequence-specific fluorescence detection formats. For the exonuclease assay and two hybridization probe sets, reproducibility and the detection limit were determined. To keep the total assay time to a minimum, we gradually shortened individual reac

APA, Harvard, Vancouver, ISO, and other styles

5

French, Cynthia, Conan Li, Charles Strom, et al. "Detection of the Factor V Leiden Mutation by a Modified Photo-Cross-Linking Oligonucleotide Hybridization Assay." Clinical Chemistry 50, no. 2 (2004): 296–305. http://dx.doi.org/10.1373/clinchem.2003.023556.

Full text

Abstract:

Abstract Background: Our previously developed assay for detection of the factor V Leiden mutation (G1691A) based on a nucleic acid photo-cross-linking technology used two allele-specific capture probes and six fluorescein-modified signal-generating reporter probes. We wished to improve the sensitivity and performance of the method. Methods: We developed new reporter probes with ∼10-fold more fluorescein molecules than the original probes. The single, cross-linker-modified capture probe was replaced by a three-probe system, separating the probe–target cross-linking function and the allelic diff

APA, Harvard, Vancouver, ISO, and other styles

6

Okano, K., and H. Kambara. "DNA Probe Assay Based on Exonuclease III Digestion of Probes Hybridized on Target DNA." Analytical Biochemistry 228, no. 1 (1995): 101–8. http://dx.doi.org/10.1006/abio.1995.1320.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Chase, Catherine J., Melanie P. Ulrich, Leonard P. Wasieloski, et al. "Real-Time PCR Assays Targeting a Unique Chromosomal Sequence of Yersinia pestis." Clinical Chemistry 51, no. 10 (2005): 1778–85. http://dx.doi.org/10.1373/clinchem.2005.051839.

Full text

Abstract:

Abstract Background: Yersinia pestis, the causative agent of the zoonotic infection plague, is a major concern as a potential bioweapon. Current real-time PCR assays used for Y. pestis detection are based on plasmid targets, some of which may generate false-positive results. Methods: Using the yp48 gene of Y. pestis, we designed and tested 2 real-time TaqMan® minor groove binder (MGB) assays that allowed us to use chromosomal genes as both confirmatory and differential targets for Y. pestis. We also designed several additional assays using both Simple-Probe® and MGB Eclipse™ probe technologies

APA, Harvard, Vancouver, ISO, and other styles

8

Guillot, Emmanuelle, and Christian Mouton. "A PCR-DNA probe assay specific forBacteroides forsythus." Molecular and Cellular Probes 10, no. 6 (1996): 413–21. http://dx.doi.org/10.1006/mcpr.1996.0057.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Rashtchian, A., J. Eldredge, M. Ottaviani, et al. "Immunological capture of nucleic acid hybrids and application to nonradioactive DNA probe assay." Clinical Chemistry 33, no. 9 (1987): 1526–30. http://dx.doi.org/10.1093/clinchem/33.9.1526.

Full text

Abstract:

Abstract Antibodies specific for DNA:RNA hybrids were coated onto polystyrene test tubes and applied to hybridization assays involving DNA and RNA. Synthetic DNA probes complementary to 16S rRNA of Campylobacter were labeled with biotin and hybridized to ribosomal RNA directly in lysates of bacterial cells. After hybridization, DNA:RNA hybrids were captured with immobilized anti-DNA:RNA antibody, and the biotinylated probe was detected with streptavidin-horseradish peroxidase (EC 1.11.1.7) conjugate. The assay was optimized to detect as few as 70,000 Campylobacter cells in a sample. We compare

APA, Harvard, Vancouver, ISO, and other styles

10

Smith, Maria, Kenneth Smith, Alan Olstein, Andrew Oleinikov, and Andrey Ghindilis. "Restriction Endonuclease-Based Assays for DNA Detection and Isothermal Exponential Signal Amplification." Sensors 20, no. 14 (2020): 3873. http://dx.doi.org/10.3390/s20143873.

Full text

Abstract:

Application of restriction endonuclease (REase) enzymes for specific detection of nucleic acids provides for high assay specificity, convenience and low cost. A direct restriction assay format is based on the specific enzymatic cleavage of a target–probe hybrid that is accompanied with the release of a molecular marker into the solution, enabling target quantification. This format has the detection limit in nanomolar range. The assay sensitivity is improved drastically to the attomolar level by implementation of exponential signal amplification that is based on a cascade of self-perpetuating r

APA, Harvard, Vancouver, ISO, and other styles

More sources

Dissertations / Theses on the topic "DNA probe assay"

1

Sumun, Faizal. "Chemiluminescent gene probes." Thesis, University of Sussex, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.352945.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Nilsson, Martina. "Mitochondrial DNA in Sensitive Forensic Analysis." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7458.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Roberts, Anthony Simon. "The cloning, characterisation and engineering of an IGF-I-BINDING single chain Fv." Queensland University of Technology, 2004. http://eprints.qut.edu.au/15914/.

Full text

Abstract:

This thesis describes the construction and characterisation of an insulin-like growth factor (IGF-I)-binding single chain Fv (scFv) and the utilisation of this scFv as a model protein for the study of the application of DNA shuffling and ribosome display to antibody engineering. The variable domain genes were isolated from the hybridoma cell line producing the monoclonal antibody and successfully joined by PCR for the construction of the scFv, named anti-GPE. Sequencing of the gene revealed an unusually short heavy chain CDR2 region. The cloned scFv was expressed in E. coli and purified. Ex

APA, Harvard, Vancouver, ISO, and other styles

4

Lin, Cheng Ming, and 林建明. "A Low Cost DNA Extraction and Purification Chip for Nucleic Acid Probe Assay." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/71646288951273424658.

Full text

Abstract:

碩士<br>國立臺灣大學<br>應用力學研究所<br>91<br>This work describes the design and development of a low cost deoxyribonucleic acid (DNA) extraction chip. The purpose of this microfludic chip is to extract DNA from environmental sample. The polydimethylsiloxane (PDMS) based device uses micromixer, microfilter and electrophoresis module for the microfluidic system, with the entire assembled device 2cm by 5cm in size. We successfully build up a portable high DC voltage power supply using a transformer and a bipolar junction transistor (BJT) to speed up the electrophoretic process. Present results show 30 second

APA, Harvard, Vancouver, ISO, and other styles

5

Cavnar, Katie Epstein Lloyd Mark. "Development of a DNA probe assay for rare transfer RNAs and its use in measuring the expression of the argX gene in Escherichia coli." 2004. http://etd.lib.fsu.edu/theses/available/etd-08192004-152949.

Full text

Abstract:

Thesis (M.S.)--Florida State University, 2004.<br>Advisor: Dr. Lloyd Epstein, Florida State University, College of Arts and Sciences, Dept. of Biological Science. Title and description from dissertation home page (viewed Jan. 12, 2005). Includes bibliographical references.

APA, Harvard, Vancouver, ISO, and other styles

6

Snelling, Anna M., Louissa Macfarlane-Smith, Jonathan N. Fletcher, and Iruka N. Okeke. "The commonly-used DNA probe for diffusely-adherent Escherichia coli cross-reacts with a subset of enteroaggregative E. coli." 2009. http://hdl.handle.net/10454/4778.

Full text

Abstract:

yes<br>Background. The roles of diffusely-adherent Escherichia coli (DAEC) and enteroaggregative E. coli (EAEC) in disease are not well understood, in part because of the limitations of diagnostic tests for each of these categories of diarrhoea-causing E. coli. A HEp-2 adherence assay is the Gold Standard for detecting both EAEC and DAEC but DNA probes with limited sensitivity are also employed. Results. We demonstrate that the daaC probe, conventionally used to detect DAEC, cross-reacts with a subset of strains belonging to the EAEC category. The cross hybridization is due to 84% identity

APA, Harvard, Vancouver, ISO, and other styles

Books on the topic "DNA probe assay"

1

International Symposium on Bioluminescence and Chemiluminescence (6th 1990 Cambridge, England). Bioluminescence and chemiluminescence: Current status : proceedings of the VIth International Symposium on Bioluminescence and Chemiluminescence, Cambridge, September 1990. Wiley, 1991.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

2

Metcalfe, Mark Andrew. Development of a solution-hybridization assay for eubacterial 16S ribosomal RNA, employing oligomers of DNA or 2'-O-allyl RNA as sequence-specific probes. University of Birmingham, 1995.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

3

Kricka, Larry J. Nonisotoic Probing, Blotting, and Sequencing, Second Edition. Academic Press, 1995.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

4

Kricka, Larry J. Nonisotoic Probing, Blotting, and Sequencing, Second Edition. 2nd ed. Academic Press, 1995.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

5

1947-, Kricka Larry J., ed. Nonisotopic probing, blotting, and sequencing. 2nd ed. Academic Press, 1995.

Find full text

APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "DNA probe assay"

1

Taylor, Ian A., and G. Geoff Kneale. "A Competition Assay for DNA Binding Using the Fluorescent Probe ANS." In Methods in Molecular Biology™. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-60327-015-1_34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Monchaud, David, and Marie-Paule Teulade-Fichou. "G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands." In Methods in Molecular Biology. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-363-9_15.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Poulin-Laprade, Dominic, and Vincent Burrus. "Electrophoretic Mobility Shift Assay Using Radiolabeled DNA Probes." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2877-4_1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Edward, Roy. "Applications and Caveats on the Utilization of DNA-Specific Probes in Cell-Based Assays." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7357-6_1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

"Analysis Techniques." In DNA Fingerprinting, edited by Lorne t. Kirby. Oxford University Press, 1993. http://dx.doi.org/10.1093/oso/9780716770015.003.0009.

Full text

Abstract:

Conventional DNA analysis techniques include cleavage of DNA by restriction enzymes, fragment electrophoresis, Southern transfer, probe labeling, probegenomic fragment hybridization, and print detection (Cawood 1989, Sambrook 1989, Berger 1987). Details of the assay conditions may vary considerably depending on the specific probes hybridized. Endonuclease digestion, electrophoresis, and Southern transfer are not required with simple dot-blot procedures. The quality of the final result can be no greater than the quality of the input DNA specimen and the attention of the analyst to assay details. The format of the analysis blot must be carefully considered to include control specimens and a broad range of size markers. The analyst must also be certain about the sizes of the profile fragments to accurately determine if matches exist between crime evidence and suspect specimen or offspring and putative parent specimens and to calculate the match probabilities. Restriction enzymes cleave DNA at specific recognition base sequences. It is important to choose an enzyme with sites flanking the repeats when fragments consisting of different numbers of tandem repeats are to be characterized for DNA profiling. Cleavage within a repeat sequence will result in the production of small fragments that may be unresolvable. The choice of enzyme, in this respect, is accomplished either by trial and error or by knowledge of the base sequence of the fragment flanking regions. The optimum reaction conditions vary for each enzyme; consequently, suppliers usually provide information sheets for the user. Digestion temperature and buffer salt concentration are the critical features. The reaction mixture can be overlaid with a few drops of paraffin oil to prevent vapor formation and changes in the buffer concentration. This applies mainly to enzymes such as Taq I that require high reaction temperatures (65°C in this example). Unless specifically indicated otherwise, three different strength ionic buffers will accommodate most enzymes.

APA, Harvard, Vancouver, ISO, and other styles

6

Graves, Steven W., and John P. Nolan. "Molecular Assemblies, Probes, and Proteomics in Flow Cytometry." In Flow Cytometry for Biotechnology. Oxford University Press, 2005. http://dx.doi.org/10.1093/oso/9780195183146.003.0013.

Full text

Abstract:

The many proteins and nucleic acids encoded in the genome predominantly perform their functions as macromolecular assemblies. In fact, modern biomedical research often targets the interactions of individual molecules of these assemblies, usually by disrupting or enhancing specific contacts, to provide treatment for many different diseases. Therefore, efficient pharmaceutical design requires knowledge of how macromolecular assemblies are built and function. To achieve this goal, sensitive and quantitative tools are essential. This chapter will discuss the use of flow cytometry as a general platform for sensitive measurement and quantification of molecular assemblies. First, this chapter will introduce general methods for analysis of molecular interactions along with a comparison of flow cytometry with these methods. Second, an overview of current flow cytometry instrumentation, assay technologies, and applications in molecular assembly analysis will be given. Third, the implementation of the above approaches in molecular assembly will be discussed. Finally, potential future directions of flow cytometry in molecular assembly analysis will be explored. At present, the analysis of macromolecular assemblies is performed by a wide variety of techniques that are chosen for the target molecules under study (proteins, DNA, lipids, etc.), the type of measurement required (kinetic or equilibrium), and whether the assembly of interest needs to be studied in vivo or in vitro. This continuum of techniques can be divided into the heterogeneous assays, which require a separation step to resolve products from reactants, and homogeneous assays, which can measure interactions without a separation step. Heterogeneous assays, in general, use radioisotopes, which are not perturbing; offer excellent sensitivity; and provide accurate quantification. The products are quantified after a separation step such as gel filtration, gel electrophoresis, or centrifugation. Rapid quench methods can provide subsecond kinetic resolution; however, the added separation steps are tedious and make collection of kinetic time courses difficult, as each time point must be separated and measured individually. Furthermore, in the time it takes the separation to occur, the interaction of interest can dissociate, which is a problem specific to low-affinity assemblies. Nonetheless, by using rapid chemical quench techniques, reaction times as short as a few milliseconds can be observed. Homogenous assays can be separated into solution- or surface-based assays. Solutionbased assays measure an optical signal generated by the assembly to quantify an interaction. High component concentrations (micromolar) allow changes in intrinsic molecular properties, such as protein fluorescence or circular dichroism, to be used to study molecular assemblies. For greater sensitivity (nanomole component concentrations), resonance energy transfer or polarization assays using exogenous fluorescent labels can be used. In combination with stopped-flow spectroscopy methodologies, solution-based assays allow reactions to monitored in a continuous fashion with submillisecond dead times.

APA, Harvard, Vancouver, ISO, and other styles

7

Zhang, Junling, Shanshan Zhao, and Jikui Wu. "Novel Biosensing Strategies for the in Vivo Detection of microRNA." In Biosensor - Current and Novel Strategies for Biosensing [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.93937.

Full text

Abstract:

As a regulatory molecule of post-transcriptional gene expression, microRNA (miRNA) is a class of endogenous, non-coding small molecule RNAs. MiRNA detection is essential for biochemical research and clinical diagnostics but challenging due to its low abundance, small size, and sequence similarities. In this chapter, traditional methods of detecting miRNA like polymerase chain reaction (PCR), DNA microarray, and northern blotting are introduced briefly. These approaches are usually used to detect miRNA in vitro. Some novel strategies for sensing miRNAs in vivo, including hybridization probe assays, strand-displacement reaction (SDR), entropy-driven DNA catalysis (EDC), catalytic hairpin assembly (CHA), hybridization chain reaction (HCR), DNAzyme-mediated assays, and CRISPR-mediated assays, are elaborated in detail. This chapter describes the principles and designs of these detection technologies and discusses their advantages as well as their shortcomings, providing guidelines for the further development of more sensitive and selective miRNA sensing strategies in vivo.

APA, Harvard, Vancouver, ISO, and other styles

8

Gilot, Philippe. "Listeriaspp.: DNA Probes and Conventional PCR Assays." In Encyclopedia of Medical Genomics and Proteomics. Informa Healthcare, 2004. http://dx.doi.org/10.3109/9780203997352.146.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Kaewphinit, Thongchai, Somchai Santiwatanakul, and Kosum Chansiri. "The Detection of Tuberculosis by Loop-Mediated Isothermal Amplification (LAMP) Combined with a Lateral Flow Dipstick." In Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering. IGI Global, 2015. http://dx.doi.org/10.4018/978-1-4666-6363-3.ch013.

Full text

Abstract:

Tuberculosis (TB) is an airborne infectious disease caused by the bacterium Mycobacterium Tuberculosis (MTB) and is a persistent problem in developing countries. Present methods for its detection include normal or nested Polymerase Chain Reaction (PCR) followed by electrophoresis, real-time PCR, Ziehl-Neelsen staining, and culture assay. These techniques entail various disadvantages such as high cost, long assay time and use of toxic substances. Novel loop-mediated isothermal amplification (LAMP) permits DNA to be amplified rapidly under constant temperature. The combination of LAMP and chromatographic Lateral Flow Dipstick (LAMP-LFD) by using biotinylated LAMP amplicon hybridized with Fluorescein Isothiocyanate (FITC)-labeled probes are allowed to detect MTB without electrophoresis and interpreted within 3-5 min. LAMP-LFD is as highly sensitive as PCR-electrophoresis method. Based on its sensitivity, specificity, rapidity, cost effectiveness, ease of use, and convenience, LAMP-LFD could be suitable for use in early MTB detection.

APA, Harvard, Vancouver, ISO, and other styles

10

"Listeria spp.: DNA Probes and Conventional PCR Assays." In Encyclopedia of Medical Genomics and Proteomics. CRC Press, 2004. http://dx.doi.org/10.1081/e-emgp-120020876.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "DNA probe assay"

1

Loftin, IR, AS McElhinny, R. Miller, et al. "P5-11-10: Reproducibility and Robustness of the FDA Approved INFORM HER2 Dual ISH DNA Probe Cocktail Assay." In Abstracts: Thirty-Fourth Annual CTRC‐AACR San Antonio Breast Cancer Symposium‐‐ Dec 6‐10, 2011; San Antonio, TX. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/0008-5472.sabcs11-p5-11-10.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Long, Yi, and Xiaoming Zhou. "Development of a simple and rapid assay for methylase activity based on DNA hairpin probe and Sybr Green I." In Photonics and Optoelectronics Meetings 2011. SPIE, 2012. http://dx.doi.org/10.1117/12.918829.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Quertermous, T., J. M. Schnee, M. S. Runge, et al. "EXPRESSION OF A RECOMBINANT ANTIBODY-TARGETED THROMBOLYTIC MOLECULE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644616.

Full text

Abstract:

We have recently shown that targeting tissue-type plasminogen activator (t-PA) by covalent linkage to a fibrin-specific monoclonal antibody (59D8) produces a more potent thrombolytic agent which also induces less fibrinogenolysis. A recombinant molecule encoding a t-PA-59D8 fusion protein was constructed to provide a ready source of this agent for further study, and to allow tailoring of the active moities for maximal activity. DNA sequence coding for the 59D8 heavy chain (HC) antigen combining site was cloned from a lambdaphage library by selection with a joining region probe. Gene segments c

APA, Harvard, Vancouver, ISO, and other styles

4

Sapolsky, Ron, Anju Shukla, Sumathi Venkatapathy, et al. "Abstract 4655: Molecular Inversion Probe analysis using OncoScan™ FFPE Assay Kit to detect copy number aberrations and somatic mutations in lung tumor DNA samples from formalin-fixed paraffin-embedded (FFPE) tissue." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-4655.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

de la Salle, C., M. J. Baas, L. Grunebaum, R. Gialeraki, T. Mandalaki, and J.-P. Cazenave. "MOLECULAR ANALYSIS OF COAGULATION FACTOR VIII AND IX GENES BY DNA PROBES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643873.

Full text

Abstract:

About 250 individuals belonging to 44 families with hemophilia A or B were studied in our laboratory. The detection of carriers was first established by pedigree analysis of each family . and coagulation and immunological assays of factor VIII or IX. The availability of specific probes for the molecular study of these two genes makes possible a diagnosis with certainty in the case of informative families. 25 families of hemophilia A were studied. For each person, blood was collected into EDTA and leucocyte DNA was extracted, digested by restriction endonucleases, electrophoresed in 0.9 % agaro

APA, Harvard, Vancouver, ISO, and other styles

6

Martins, Diogo, Xi Wei, Rastislav Levicky, and Yong-Ak Song. "Accelerating the Mass Transport of DNA Biomolecules Onto DNA Microarray for Enhanced Detection by Electrokinetic Concentration in a Microfluidic Chip." In ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/mnhmt2016-6562.

Full text

Abstract:

Morpholinos (MOs) are synthetic nucleic acids analogues with a non-charged backbone of morpholine rings. To enhance the MO-DNA hybridization assay speed, we propose the integration of a MO microarray with an ion concentration polarization (ICP) based microfluidic concentrator. The ICP concentrator collects target biomolecules from a ∼μL fluidic DNA sample and concentrates them electrokinetically into a ∼nL plug located in the vicinity of the MO probes. ICP preconcentration not only reduces the analyte diffusion length but also increases the binding reaction rate, and as a result, ICP-enhanced

APA, Harvard, Vancouver, ISO, and other styles

7

Ploos van Amstel, J. K., A. L. van der Zanden, P. H. Reitsma, and R. M. Bertina. "RESTRICTION ANALYSIS AND SOUTHERN BLOTTING OF TOTAL HUMAN DNA REVEALS THE EXISTENCE OF MORE THAN ONE GENE HOMOLOGOUS WITH PROTEIN S cDNA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644639.

Full text

Abstract:

A deficiency in protein S, the cofactor of activated protein C, is associated with an increased risk for the development of venous thrombosis. It is inherited as an autosomal dominant disorder. To improve the detection of heterozygotes in affected families, we have started to search for restriction fragment length polymorphism (RFLP) in the protein S gene. This study revealed the existence of two genes containing sequences homologous to protein S cDNA.Three non-overlapping fragments of clone pSUL5, which codes for the carboxy-terminal part of protein S and contains the complete 3' untranslated

APA, Harvard, Vancouver, ISO, and other styles

8

Rajput, B., D. Alaimo, A. M. Asselbergs, and E. Reich. "CONSTRUCTION AND EXPRESSION OF HYBRID PLASMINOGEN ACTIVATOR GENES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644412.

Full text

Abstract:

Hybrid plasminogen activator (PA) genes containing fragments of cDNA encoding the non-catalytic part of tissue-PA and the .catalytic domain of urokinase and vice versa were constructed and expressed in Chinese Hamster ovary (CHO) cells. The hybrid nature of the products in stably transformed cells was analyzed at the level of DNA and RNA using probes derived from different regions of the urokinase and tissue-PA cDNAs and at the protein level by means of polyclonal antibodies raised against tissue-PA and urokinase. The hybrid genes made hybrid RNAs and proteins of the expected sizes. The protei

APA, Harvard, Vancouver, ISO, and other styles

9

Wang, Yuker, Ron Sapolsky, Jen Wilkins, Victoria Carlton, Farooq Siddiqui, and Tom Asbury. "Abstract B14: A high-throughput allelic copy-number platform utilizing a 75-ng DNA input in a 330,000 molecular inversion probes (MIP) assay on microarrays with FFPE samples." In Abstracts: AACR International Conference on Translational Cancer Medicine-- Jul 11-14, 2010; San Francisco, CA. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1078-0432.tcmusa10-b14.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Wang, Yuker, Ron Sapolsky, Sumathi Venkatapathy, Farooq Siddiqui, and Fan Shen. "Abstract 4868: A high-throughput allelic copy-number and somatic mutation platform utilizing a 75-ng DNA input in a 330,000 Molecular Inversion Probes (MIP) assay with FFPE samples." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-4868.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!