Relevant bibliographies by topics / DNA replication – Research – Methodology

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'DNA replication – Research – Methodology'

Author: Grafiati
Published: 4 June 2021
Last updated: 11 February 2022

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Journal articles on the topic "DNA replication – Research – Methodology"

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Kristian, Dolly Simon, Adian Fatchur Rochim, and Eko Didik Widianto. "Pengembangan Sistem Replikasi dan Redundansi untuk Meningkatkan Kehandalan Basisdata MySQL." Jurnal Teknologi dan Sistem Komputer 3, no. 4 (October 20, 2015): 523. http://dx.doi.org/10.14710/jtsiskom.3.4.2015.523-529.

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With the development of information technology, humans make it easy to complete every duty. Any information about the work carried out to be very valuable, therefore the information should be stored properly, by organizing a reliable database system when performing data storage on the server information. Build a design application virtualization master slave database servers that are connected with the management node using the virtual application to get the test results replication system performance and redundancy in the design of the cluster system. Methodology of this research include the study of literature, collecting data by interview, observation, literature studies, system design, and testing of the system. In a literature study on the use of research methods to study the literature books, records that can be used as a support in the research. The design of this thesis using MySQL Cluster system with Ndbcluster engine. Last is testing this system on its performance on the server failure or failures occur and reliability in performance. The results obtained are when there is a failure on the primary server, it will be immediately replaced by another server is a slave. And the replication of data between the main server and slave.
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Fabrice, Antigny, Ranchoux Benoît, Nadeau Valérie, Edmund Lau, Bonnet Sébastien, and Perros Frédéric. "A Simple Method to AssessIn VivoProliferation in Lung Vasculature with EdU: The Case of MMC-Induced PVOD in Rat." Analytical Cellular Pathology 2015 (2015): 1–6. http://dx.doi.org/10.1155/2015/326385.

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5-Ethynyl-2′-deoxyuridine (EdU) incorporation is becoming the gold standard method forin vitroandin vivovisualization of proliferating cells. The small size of the fluorescent azides used for detection results in a high degree of specimen penetration. It can be used to easily detect DNA replication in large tissue samples or organ explants with low proliferation and turnover of cells formerly believed to be in a “terminal” state of differentiation. Here we describe a protocol for the localization and identification of proliferating cells in quiescent or injured pulmonary vasculature, in a model of pulmonary veno-occlusive disease (PVOD). PVOD is an uncommon form of pulmonary hypertension characterized by progressive obstruction of small pulmonary veins. We previously reported that mitomycin-C (MMC) therapy is associated with PVOD in human. We demonstrated that MMC can induce PVOD in rats, which currently represents the sole animal model that recapitulates human PVOD lesions. Using the EdU assay, we demonstrated that MMC-exposed lungs displayed areas of exuberant microvascular endothelial cell proliferation which mimics pulmonary capillary hemangiomatosis, one of the pathologic hallmarks of human PVOD.In vivopulmonary cell proliferation measurement represents an interesting methodology to investigate the potential efficacy of therapies aimed at normalizing pathologic angioproliferation.
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Dhawan, Andrew, Justin Lathia, David Peereboom, Gene Barnett, Gabrielle Yeaney, and Manmeet Ahluwalia. "COMP-17. LARGE-SCALE TRANSCRIPTOMIC CHARACTERIZATION OF PRIMARY AND RECURRENT GLIOBLASTOMA IDENTIFIES GENE EXPRESSION SIGNATURE OF TUMOR RESPONSE TO STANDARD THERAPY." Neuro-Oncology 21, Supplement_6 (November 2019): vi64—vi65. http://dx.doi.org/10.1093/neuonc/noz175.260.

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Abstract A near-universal phenomenon in glioblastoma is disease recurrence following surgical resection and chemoradiotherapy. Development of biomarkers predictive of therapeutic response to better guide care and inform future targeted therapies is crucial. In this work, a total of 84 glioblastoma surgical specimens involving 44 primary tumors and 40 matched samples at time of re-resection, were characterized utilizing RNA-sequencing. Transcriptomic analysis was carried out with the goal of identifying underlying differences between those patients with prolonged response to standard therapy and delayed time to re-resection. We examined individual gene expression, gene coexpression networks, and well-known gene pathways in this dataset that showed consistent association with time to re-resection in both primary and progressed specimens, independent of tumor molecular subtype. Leveraging this large, well-characterized dataset, and using a novel computational methodology based on a seed-gene approach, we identified a predictive gene signature for therapeutic response. Our analyses revealed a striking degree of heterogeneity among gene expression associated with response to standard therapy and time to re-resection, adding to the complexity of signature derivation. The novel signature we obtained for response showed components involving genes such as those in the IGF pathway (IGF2BP2, IGF2BP3) and PDGF-signalling pathway (MYC, FLI1, ARHGAP4, JAK3) predictive of poor response to therapy. Likewise, predictors of positive response to therapy included genes involved in the apoptosis and RAS pathways (RAB4A, CHUK) and DNA replication pathways (SSBP2). In sum, this is among the largest cohorts of well-characterized clinical tumor samples for which there is transcriptomic information from primary and re-resected samples from matched patients. Our results not only highlight an innovative computational method for gene signature derivation in the setting of significant underlying heterogeneity, but also result in a predictive gene signature, offering the potential to give therapy to those who stand to benefit most.
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Preston, Bradley D., Tina M. Albertson, and Alan J. Herr. "DNA replication fidelity and cancer." Seminars in Cancer Biology 20, no. 5 (October 2010): 281–93. http://dx.doi.org/10.1016/j.semcancer.2010.10.009.

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Li, Zhuo, Lori M. Kelman, and Zvi Kelman. "Thermococcus kodakarensis DNA replication." Biochemical Society Transactions 41, no. 1 (January 29, 2013): 332–38. http://dx.doi.org/10.1042/bst20120303.

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DNA replication plays an essential role in all life forms. Research on archaeal DNA replication began approximately 20 years ago. Progress was hindered, however, by the lack of genetic tools to supplement the biochemical and structural studies. This has changed, however, and genetic approaches are now available for several archaeal species. One of these organisms is the thermophilic euryarchaeon Thermococcus kodakarensis. In the present paper, the recent developments in the biochemical, structural and genetic studies on the replication machinery of T. kodakarensis are summarized.
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Ekundayo, Babatunde, and Franziska Bleichert. "Origins of DNA replication." PLOS Genetics 15, no. 9 (September 12, 2019): e1008320. http://dx.doi.org/10.1371/journal.pgen.1008320.

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Zhukovskaya, N., P. Branch, G. Aquilina, and P. Karran. "DNA replication arrest and tolerance to DNA methylation damage." Carcinogenesis 15, no. 10 (1994): 2189–94. http://dx.doi.org/10.1093/carcin/15.10.2189.

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Henricksen, Leigh A., and Robert A. Bambara. "Multiprotein reactions in mammalian DNA replication." Leukemia Research 22, no. 1 (January 1998): 1–5. http://dx.doi.org/10.1016/s0145-2126(97)00113-6.

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Arana, Mercedes E., and Thomas A. Kunkel. "Mutator phenotypes due to DNA replication infidelity." Seminars in Cancer Biology 20, no. 5 (October 2010): 304–11. http://dx.doi.org/10.1016/j.semcancer.2010.10.003.

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Desaintes, Christian, and Caroline Demeret. "Control of papillomavirus DNA replication and transcription." Seminars in Cancer Biology 7, no. 6 (December 1996): 339–47. http://dx.doi.org/10.1006/scbi.1996.0043.

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Dissertations / Theses on the topic "DNA replication – Research – Methodology"

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Wendel, Brian Michael. "Completion of DNA Replication in Escherichia coli." PDXScholar, 2018. https://pdxscholar.library.pdx.edu/open_access_etds/4406.

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To maintain genomic integrity, all cells must accurately duplicate their genetic material in order to provide intact and complete copies to each daughter cell following cell division. Successful inheritance of chromosomal information without changing even a single nucleotide requires accurate and robust DNA replication. This requires that cells tightly control replication initiation from the origin(s), processive elongation of the replisome, and the completion of DNA replication by resolving convergent replication forks ensuring that each sequence is duplicated without alteration. Unlike initiation and elongation, the process by which replication forks converge and are resolved into two discrete, inheritable DNA molecules is not well understood. This process must be remarkably efficient, occurring thousands of times per cell division in human cells, and is likely to be a fundamental step in regulating genome stability in all cells. In this dissertation I address how DNA replication completes in the model system Escherichia coli. To achieve this, I examined candidate mutants for impairments in the completion of DNA replication. By evaluating growth, viability, chromosomal copy number, and plasmid stability I identified a requirement for the proteins RecBCD, ExoI, and SbcCD in the completion reaction. SbcCD and ExoI act before RecBCD in the completion reaction and process the DNA intermediates arising as replication forks converge. These enzymes act in the completion reaction without recombination or RecA, but in the absence of the normal process recombination is required to complete DNA replication via an aberrant pathway that results in genomic instability.
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Hamilton, Nicklas Alexander. "Use of Two-replisome Plasmids to Characterize How Chromosome Replication Completes." PDXScholar, 2019. https://pdxscholar.library.pdx.edu/open_access_etds/5064.

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All living organisms need to accurately replicate their genome to survive. Genomic replication occurs in three phases; initiation, elongation, and completion. While initiation and elongation have been extensively characterized, less is known about how replication completes. In Escherichia coli completion occurs at sites where two replication forks converge and is proposed to involve the transiently bypass of the forks, before the overlapping sequences are resected and joined. The reaction requires RecBCD, and involves several other gene products including RecG, ExoI, and SbcDC but can occur independent of recombination or RecA. While several proteins are known to be involved, how they promote this reaction and the intermediates that arise remain uncharacterized. In the first part of this work, I describe the construction of plasmid "mini-chromosomes" containing a bidirectional origin of replication that can be used to examine the intermediates and factors required for the completion reaction. I verify that these substrates can be used to study the completion reaction by demonstrating that these plasmids require completion enzymes to propagate in cells. The completion enzymes are required for plasmids containing two-replisomes, but not one replisome, indicating that the substrate these enzymes act upon in vivo is specifically created when two replication forks converge. Completion events in E. coli are localized to one of the six termination (ter) sequences within the 400-kb terminus region due to the autoregulated action of Tus, which binds to ter and inhibits replication fork progression in an orientation-dependent manner. In the second part of this work, I examine how the presence of ter sequences affect completion on the 2-replisome plasmid. I show that addition of ter sequences modestly decreases the stability of the two-replisome plasmid and that this correlates with higher levels of abnormal, amplified molecules. The results support the idea that ter sites are not essential to completion of DNA replication; similar to what is seen on the chromosome. Rec-B-C-D forms a helicase-nuclease complex that, in addition to completion, is also required for double-strand break repair in E. coli. RecBCD activity is altered upon encountering specific DNA sequences, termed chi, in a manner that promotes crossovers during recombinational processes. In the third part of this work, I demonstrate that the presence of chi in a bidirectional plasmid model promotes the appearance of over-replicated linear molecules and that these products correlate with a reduced stability of the plasmid. The effect appears specific to plasmids containing two replisomes, as chi on the leading or lagging strand of plasmids containing one replisome had no-effect. The observation implies chi promotes a reaction that may encourage further synthesis during the completion reaction, and that at least on the mini-chromosomes substrates, this appears to be a destabilizing force.
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Godfrey, D. B. "Cloning of a gene involved in replication of DNA on a damaged template." Thesis, University of Cambridge, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259596.

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Yao, Jianhong. "DUE-B, A NEW HUMAN DNA REPLICATION PROTEIN, IS THE FUNCTIONAL HOMOLOG OF S. CEREVISIAE SLD3." Wright State University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=wright1238526547.

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Poudel, Sumeet. "Interaction of DUE-B and Treslin during the initiation of DNA replication." Wright State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=wright1484841026310282.

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Chen, Xiaomi. "Aberrant DNA Replication at an Ectopic Chromosomal Site in Human Cells." Wright State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=wright1302884072.

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Xie, Dan, and 謝丹. "Application of high-throughput tissue microarray technology in cancer research." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2004. http://hub.hku.hk/bib/B30283619.

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Serrano, Moises A. "Novel Roles of Replication Protein A Phosphorylation in Cellular Response to DNA Damage." Digital Commons @ East Tennessee State University, 2013. https://dc.etsu.edu/etd/1206.

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Human replication protein A (RPA) is an eukaryotic single-stranded DNA binding protein directly involved in a variety of DNA metabolic pathways including replication, recombination, DNA damage checkpoints and signaling, as well as all DNA repair pathways. This project presents 2 novel roles of RPA in the cellular response to DNA damage. The first elucidates the regulation of RPA and p53 interaction by DNA-dependent protein kinase (DNA-PK), ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) in homologous recombination (HR). HR and nonhomologous end joining (NHEJ) are 2 distinct DNA double-stranded break (DSB) repair pathways. Here, we report that DNA-PK, the core component of NHEJ, partners with DNA-damage checkpoint kinases ATM, and ATR to synergistically regulate HR repair of DSBs. The regulation was accomplished through modulation of the p53-RPA interaction. We show that upon DNA damage p53 and RPA are freed from the p53–RPA complex. This is done through simultaneous phosphorylation of RPA by DNA-PK, and p53 by ATR and ATM. Neither the phosphorylation of RPA nor that of p53 alone could dissociate the p53-RPA complex; furthermore, disruption of the release significantly compromised HR repair of DSBs. Our results reveal a mechanism for the crosstalk between HR and NHEJ repair through the coregulation of p53–RPA interaction by DNA-PK, ATM and ATR. The second part of this project reveals a novel role of RPA32 phosphorylation in suppressing the signaling of programmed cell death, also known as apoptosis. Our results show that deficiency in RPA32 phosphorylation leads to increased apoptosis after genotoxic stress. Specifically, PARP-1 cleavage, Caspase-3 activation, sub-G1 cell population, annexin V staining and the loss of mitochondrial membrane potential were significantly increased in the phospho-deficient RPA32 cells (PD-RPA32). The lack of RPA phosphorylation also promoted activation of initiator Caspase-9 and effector Caspase-3 and -7. This regulation is dependent on the kinase activity of DNA-PK and is mediated by PUMA through the ATM-p53 pathway. Our results suggest a novel role of RPA phosphorylation in apoptosis that illuminates a new target that lies on the crossroads of DNA repair and cell death, a pivotal point that could be of importance for sensitizing cancer cells to chemotherapy.
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Tower, Dallas Lauren. "The effect of putative vesicular stomatitis virus methyltransferase mutants on transcription and replication." [Gainesville, Fla.] : University of Florida, 2005. http://purl.fcla.edu/fcla/etd/UFE0010088.

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Thesis (M.S.)--University of Florida, 2005.
Typescript. Title from title page of source document. Document formatted into pages; contains 57 pages. Includes Vita. Includes bibliographical references.
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Strahl, Audra Lynne. "Characterization of temperature sensitive vaccinia virus mutants in the a3l and e6r complementation groups." [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0005203.

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Thesis (M.S.)--University of Florida, 2004.
Typescript. Title from title page of source document. Document formatted into pages; contains 59 pages. Includes Vita. Includes bibliographical references.
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Books on the topic "DNA replication – Research – Methodology"

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Vengrova, Sonya, and Jacob Z. Dalgaard. DNA replication: Methods and protocols. New York: Humana Press, 2015.

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DNA replication: Methods and protocols. Totowa, N.J: Humana, 2009.

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Snapka, Robert M. The SV40 replicon model for analysis of anticancer drugs. Austin, TX: R.G. Landes, 1996.

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The SV40 replicon model for analysis of anticancer drugs. [San Diego, Calif.]: Academic Press, 1996.

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Metzenberg, Stan. Working with DNA. New York, NY: Taylor & Francis, 2006.

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John, Quackenbush, and Brazma Alvis, eds. Microarray gene expressions data analysis: A beginner's guide. Oxford: Blackwell, 2003.

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Media, Springer Science+Business, ed. DNA vaccines: Methods and protocols. New York: Humana Press, 2014.

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Göhlmann, Hinrich. Gene expression studies using affymetrix microarrays. Boca Raton: Taylor & Francis, 2009.

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DNA microarrays for biomedical research: Methods and protocols. New York: Humana Press, 2009.

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DNA damage detection in situ, ex vivo, and in vivo: Methods and protocols. New York: Humana Press, 2011.

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Book chapters on the topic "DNA replication – Research – Methodology"

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Gast, David L., and Jennifer R. Ledford. "Replication." In Single Case Research Methodology, 77–95. Third Edition. | New York : Routledge, 2018. | Revised edition of Single case research methodology, 2014.: Routledge, 2018. http://dx.doi.org/10.4324/9781315150666-4.

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Abbuhl, Rebekha. "Research Replication." In The Palgrave Handbook of Applied Linguistics Research Methodology, 145–62. London: Palgrave Macmillan UK, 2018. http://dx.doi.org/10.1057/978-1-137-59900-1_7.

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Chong, James P. J., and J. Julian Blow. "DNA replication licensing factor." In Progress in Cell Cycle Research, 83–90. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4615-5873-6_8.

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Burhans, William C., and Martin Weinberger. "DNA DamageDNA damage and DNA Replication StressDNA replication stress in Yeast Models of Aging." In Aging Research in Yeast, 187–206. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-2561-4_9.

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Fotedar, Rati, and Arun Fotedar. "Cell cycle control of DNA replication." In Progress in Cell Cycle Research, 73–89. Boston, MA: Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-1809-9_6.

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Los, Dmitry A., Michael P. Malakhov, and Victor E. Semenenko. "Dunaliella Salina Chloroplast DNA Fragment Maintains Initiation and Termination of DNA Replication in E.Coli." In Research in Photosynthesis, 303–6. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-009-0383-8_67.

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Nishitani, Hideo, and Paul Nurse. "The cdc18 protein initiates DNA replication in fission yeast." In Progress in Cell Cycle Research, 135–42. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-5371-7_11.

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Tourret, Jérôme, and Frank McKeon. "Tyrosine kinases wee1 and mik1 as effectors of DNA replication checkpoint control." In Progress in Cell Cycle Research, 91–97. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4615-5873-6_9.

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Seth, Banabithi Koley, and Samita Basu. "Magnetic Field Effect on Photoinduced Interactions: Its Implications in Distance-Dependent Photoinduced Electron Transfer Between CT-DNA and Metal Complex." In Research Methodology in Chemical Sciences, 1–15. Toronto : Apple Academic Press, 2016.: Apple Academic Press, 2017. http://dx.doi.org/10.1201/9781315366616-1.

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Richer, C. L., and R. Drouin. "Dynamic Banding for High-Resolution Analysis of Chromosomes and Assignment of DNA Replication Times." In Advances in Mutagenesis Research, 55–94. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-75599-6_2.

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Conference papers on the topic "DNA replication – Research – Methodology"

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Barger, Carter J., Linda Chee, Connor Branick, Kunle Odunsi, Ronny Drapkin, and Adam R. Karpf. "Abstract B36: FOXM1 induces DNA replication stress, and its bidirectional gene partner RHNO1 participates in the DNA replication stress response, in high-grade serous ovarian cancer." In Abstracts: AACR Special Conference: Addressing Critical Questions in Ovarian Cancer Research and Treatment; October 1-4, 2017; Pittsburgh, PA. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1557-3265.ovca17-b36.

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Contreras Landeros, Selene Yazmín, Francisco Pérez Mariscal, Lucero León Rangel, and América Nitxin Castañeda Sortibrán. "TEACHING-LEARNING STRATEGY BASED ON CASE ANALYSIS FOR "REPLICATION, TRANSCRIPTION AND TRANSLATION OF DNA" (HIGH SCHOOL STUDENTS)." In 10th annual International Conference of Education, Research and Innovation. IATED, 2017. http://dx.doi.org/10.21125/iceri.2017.1059.

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Barger, Carter J., Kajal Biswas, Linda Chee, Ronny Drapkin, Shyam Sharan, and Adam R. Karpf. "Abstract AP12: FOXM1 INDUCES DNA REPLICATION STRESS AND PROMOTES GENOMIC INSTABILITY DOWNSTREAM OF CYCLIN E1 IN HIGH-GRADE SEROUS OVARIAN CANCER." In Abstracts: 12th Biennial Ovarian Cancer Research Symposium; September 13-15, 2018; Seattle, Washington. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1557-3265.ovcasymp18-ap12.

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Tomkova, M., M. McClellan, S. Kriaucionis, and B. Schuster-Boeckler. "PO-328 No such thing as a perfect copy – DNA replication profoundly influences how most environmental carcinogens and epigenetic marks induce mutations." In Abstracts of the 25th Biennial Congress of the European Association for Cancer Research, Amsterdam, The Netherlands, 30 June – 3 July 2018. BMJ Publishing Group Ltd, 2018. http://dx.doi.org/10.1136/esmoopen-2018-eacr25.358.

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Eltai, Nahla Omer, Hadi M. Yassine, Sara H. Al-Hadidi, Tahra ElObied, Asmaa A. Al Thani, and Walid Q. Alali. "Retail Chicken Carcasses as a Reservoir of Antimicrobial- Resistant Escherichia coli." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0115.

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The dissemination of antimicrobial resistance (AMR) bacteria has been associated with the inappropriate use of antibiotics in both humans and animals and with the consumption of food contaminated with resistant bacteria. In particular, the use of antibiotics as prophylactic and growth promotion purposes in food-producing animals has rendered many of the antibiotics ineffective. The increased global prevalence of AMR poses a significant threat to the safety of the world’s food supply. Objectives: This study aims at determining the prevalence of antibiotic-resistant Escherichia coli (E. coli) isolated from local and imported retail chicken meat in Qatar. Methodology: A total of 270 whole chicken carcasses were obtained from three different hypermarket stores in Qatar. A total of 216 E. coli were isolated and subjected to antibiotic susceptibility testing against 18 relevant antibiotics using disc diffusion and micro- dilution methods. Furthermore, extended-spectrum β-lactamase (ESBL) production was determined via a double-disc synergetic test. Isolates harboring colistin resistance were confirmed using multiplex-PCR and DNA sequencing. Results: Nearly 89% (192/216) of the isolates were resistant to at least one antibiotics. In general, isolates showed relatively higher resistance to sulfamethoxazole (62%), tetracycline (59.7%), ampicillin and trimethoprim (52.3%), ciprofloxacin (47.7%), cephalothin, and colistin (31.9%). On the other hand, less resistance was recorded against amoxicillin/clavulanic acid (6%), ceftriaxone (5.1%), nitrofurantoin (4.2%) and piperacillin/tazobactam (4.2%), cefepime (2.3%), meropenem (1.4%), ertapenem (0.9%), and amikacin (0.9%). Nine isolates (4.2%) were ESBL producers. Furthermore, 63.4% were multidrug-resistant (MDR). The percentage of MDR, ESBL producers, and colistin-resistant isolates was significantly higher among local isolates compared to imported chicken samples. Conclusion: We reported a remarkably high percentage of the antibiotic-resistant E. coli in chicken meat sold at retail in Qatar. The high percentage of MDR and colistin isolates is troublesome to the food safety of raw chicken meat and the potential of antibiotic resistance spread to public health. Our findings support the need for the implementation of one health approach to address the spread of antimicrobial resistance and the need for a collaborative solution.
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Frohnapfel, Dustin J., K. Todd Lowe, and Walter F. O’Brien. "Development, Analysis, and Validation of a Simultaneous Inlet Total Pressure and Swirl Distortion Generator." In ASME Turbo Expo 2020: Turbomachinery Technical Conference and Exposition. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/gt2020-16125.

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Abstract Over the last decade, the Turbomachinery and Propulsion Research Laboratory at Virginia Tech has researched, invented, developed, computationally analyzed, experimentally tested, and improved turbofan engine inlet distortion generators. This effort began with modernizing and improving inlet total pressure distortion screens originally conceived over half a century ago; continued with the invention of inlet swirl distortion generators (StreamVanes™) made possible only through advances in modern additive manufacturing technology; and has, thus far, culminated in a novel combined device (ScreenVanes™) capable of simulating realistic flight conditions of coupled inlet total pressure and swirl distortion in a ground-test turbofan engine research platform. The present research focuses on the methodology development, computational analysis, and experimental validation of a novel simultaneous inlet total pressure and swirl distortion generator. A case study involving a single bend S-duct inlet distortion profile demonstrates the ability to generate a high-fidelity profile simulation, yet outlines a design process sufficiently generic for application to any arbitrary inlet geometry or distortion profile. A computational fluid dynamics simulation of the S-duct inlet provided the target profile extracted at the aerodynamic interface plane. Next, utilizing a method of inverse propagation, the planar distortion profile was propagated upstream to yield a flow field that could be manufactured by a distortion generator adequately isolated from turbomachinery effects. The total pressure distortion screen and swirl distortion StreamVane components were then designed and computationally analyzed. Upon successful computational reproduction of the S-duct inlet distortion profile, experimental hardware was fabricated and tested to validate the ScreenVane methodology and distortion generating device. Comparison of the S-duct manufactured distortion and the ScreenVane manufactured distortion was used as the primary criterion for profile replication success. Results from a computational analysis of both the S-duct and ScreenVane indicated excellent agreement in distortion pattern shape, extent, and intensity with full-field total pressure recovery and swirl angle profiles matching within approximately 0.80% and 2.6°, respectively. Furthermore, experimental validation of the ScreenVane indicated nearly identical full-field total pressure recovery and swirl angle profile replication of approximately 1.10% and 2.6°, respectively, when compared to the computational results. The investigation concluded that not only was the ScreenVane device capable of accurately simulating a complex inlet distortion profile, but also produced a viable device for full-scale turbofan engine ground test.
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Farrugia, P. J., P. Vella, and M. Mifsud. "A Framework Supporting Life-Cycle Guidance for Fabricating Micro Components by Compression Moulding." In ASME 2010 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2010. http://dx.doi.org/10.1115/detc2010-28248.

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Micro-products and components are rapidly increasing in a range of sectors. This demand requires industrial technologies capable of mass producing polymer micro products at a reasonable price. These two requirements are satisfied by the use of replication technologies such as micro compression moulding (μCM). A literature review that we conducted revealed that there is indeed little if any work explicitly related to the domain of micro compression moulding. In addition, a design methodology in micro manufacturing is still in its infancy and that there is a shortage of relevant DFX guidelines. Thus, this research aims at developing a framework for a computer-based tool whereby micro-product stakeholders are guided to select the correct mould features, material, machine and process parameters for fabricating components via micro compression moulding. The paper presents a framework developed to meet this goal. A proof-of-concept tool has also been implemented based on this framework. This tool was evaluated by typical case studies and also presented to a number of experts in the field. Preliminary evaluation results provide a degree of evidence that technology based on the framework contributes a step towards providing guidance for the design and manufacture of mould tools for fabricating μ-components by compression moulding. Another contribution of this paper is the preliminary fabrication platform using μCM. Future work is however required mainly to assess the economic feasibility of the fabrication platform, to address the limitations of the implemented tool, and to assess its effectiveness in practice.
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