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Journal articles on the topic 'DNA topoisomerasa II beta'

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Published: 28 June 2021

Last updated: 29 July 2025

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1

Cowell, Ian G., John W. Casement, and Caroline A. Austin. "To Break or Not to Break: The Role of TOP2B in Transcription." International Journal of Molecular Sciences 24, no. 19 (2023): 14806. http://dx.doi.org/10.3390/ijms241914806.

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Transcription and its regulation pose challenges related to DNA torsion and supercoiling of the DNA template. RNA polymerase tracking the helical groove of the DNA introduces positive helical torsion and supercoiling upstream and negative torsion and supercoiling behind its direction of travel. This can inhibit transcriptional elongation and other processes essential to transcription. In addition, chromatin remodeling associated with gene activation can generate or be hindered by excess DNA torsional stress in gene regulatory regions. These topological challenges are solved by DNA topoisomeras

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2

Sharma, Kaushal K., Brijendra Singh, Somdutt Mujwar та Prakash S. Bisen. "Molecular Docking Based Analysis to Elucidate the DNA Topoisomerase IIβ as the Potential Target for the Ganoderic Acid; A Natural Therapeutic Agent in Cancer Therapy". Current Computer-Aided Drug Design 16, № 2 (2020): 176–89. http://dx.doi.org/10.2174/1573409915666190820144759.

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Introduction: Intermediate covalent complex of DNA-Topoisomerase II enzyme is the most promising target of the anticancer drugs to induce apoptosis in cancer cells. Currently, anticancer drug and chemotherapy are facing major challenges i.e., drug resistance, chemical instability and, dose-limiting side effect. Therefore, in this study, natural therapeutic agents (series of Ganoderic acids) were used for the molecular docking simulation against Human DNATopoisomerase II beta complex (PDB ID:3QX3). Methods: Molecular docking studies were performed on a 50 series of ganoderic acids reported in t

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3

Bernard, F. X., S. Sablé, B. Cameron, et al. "Glycosylated flavones as selective inhibitors of topoisomerase IV." Antimicrobial Agents and Chemotherapy 41, no. 5 (1997): 992–98. http://dx.doi.org/10.1128/aac.41.5.992.

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Three flavonoids which promoted Escherichia coli topoisomerase IV-dependent DNA cleavage were isolated from cottonseed flour and identified as quercetin 3-O-beta-D-glucose-[1,6]-O-alpha-L-rhamnose (rutin), quercetin 3-O-beta-D-galactose-[1,6]-O-alpha-L-rhamnose, and quercetin 3-O-beta-D-glucose (isoquercitrin). The most active one (rutin) also inhibited topoisomerase IV-dependent decatenation activity (50% inhibitory concentration, 64 microg/ml) and induced the SOS response of a permeable E. coli strain. Derivatives of quercetin glycosylated at position C-3 were shown to induce two site-specif

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4

Nitiss, Karin C., Brigid Conroy, Maureen N. McCoy, and John L. Nitiss. "Abstract 2838: Modeling putative oncogenic variants of human topoisomerase II by genome editing of a near haploid cell line." Cancer Research 85, no. 8_Supplement_1 (2025): 2838. https://doi.org/10.1158/1538-7445.am2025-2838.

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DNA topoisomerases (Topos) carry out changes in DNA structure by introducing transient breaks in DNA. These breaks are needed to change DNA conformation, and the enzyme mechanisms minimize possible genome instability from a failure to resolve the cleaved DNA intermediates. Nonetheless, small molecules can interfere with the Topo reaction, leading to elevated DNA cleavage, genome instability and cell death, and this property is the basis of the action of important anti-cancer drugs. Recent work has led to the identification of mutations in eukaryotic Top2 that also result in high levels of Top2

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5

Jenkins, J. R., M. J. Pocklington, and E. Orr. "The F1 ATP synthetase beta-subunit: a major yeast novobiocin binding protein." Journal of Cell Science 96, no. 4 (1990): 675–82. http://dx.doi.org/10.1242/jcs.96.4.675.

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Novobiocin affects DNA metabolism in both prokaryotes and eukaryotes, resulting in cell death. In prokaryotes, the drug is a specific inhibitor of DNA gyrase, a type II topoisomerase that can be purified on a novobiocin-Sepharose column. The yeast type II topoisomerase is neither the biochemical, nor the genetic target of the antibiotic. We have purified the major yeast novobiocin binding proteins and identified one of them as the beta-subunit of the yeast mitochondrial F1 ATP synthetase, a protein highly conserved throughout evolution. The inactivation of this protein might explain the toxic

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6

Strehl, Sabine, Karin Nebral, Helmut H. Schmidt, and Oskar A. Haas. "Topoisomerase (DNA) II Beta 180 kDa TOP2B) - A New NUP98 Fusion Partner." Blood 106, no. 11 (2005): 2849. http://dx.doi.org/10.1182/blood.v106.11.2849.2849.

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Abstract The nucleoporin 98 kDa (NUP98) gene has been reported to be fused to 18 different partner genes in various hematological malignancies with 11p15 aberrations. The most frequently observed fusion partners of NUP98 belong to the homeobox family of transcription factors, whereas the non-HOX NUP98 fusion partners comprise a heterogeneous group of genes that are associated with a wide range of biological functions. Cytogenetic analysis of an adult de novo acute myeloid leukemia (AML-M5a) revealed a t(3;11)(p24;p15) indicating fusion of NUP98 with a novel partner gene. Fluorescence in situ h

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7

Biersack, H., S. Jensen, I. Gromova, I. S. Nielsen, O. Westergaard, and A. H. Andersen. "Active heterodimers are formed from human DNA topoisomerase II alpha and II beta isoforms." Proceedings of the National Academy of Sciences 93, no. 16 (1996): 8288–93. http://dx.doi.org/10.1073/pnas.93.16.8288.

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8

Muller, M. T., and V. B. Mehta. "DNase I hypersensitivity is independent of endogenous topoisomerase II activity during chicken erythrocyte differentiation." Molecular and Cellular Biology 8, no. 9 (1988): 3661–69. http://dx.doi.org/10.1128/mcb.8.9.3661-3669.1988.

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Endogenous topoisomerase II cleavage sites were mapped in the chicken beta A-globin gene of 12- to 14-day embryonic erythrocytes. A major topoisomerase II catalytic site was mapped to the 5' end of the globin gene which contained a nucleosome-free and DNase I-hypersensitive site and additional but minor sites were mapped to the second intron and 3' of the gene to a tissue-specific enhancer. Cleavage sites, mapped in situ by indirect end labeling, were aligned to single-base-pair resolution by comparison to a consensus sequence derived for vertebrate topoisomerase II catalytic sites. In contras

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9

Muller, M. T., and V. B. Mehta. "DNase I hypersensitivity is independent of endogenous topoisomerase II activity during chicken erythrocyte differentiation." Molecular and Cellular Biology 8, no. 9 (1988): 3661–69. http://dx.doi.org/10.1128/mcb.8.9.3661.

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Endogenous topoisomerase II cleavage sites were mapped in the chicken beta A-globin gene of 12- to 14-day embryonic erythrocytes. A major topoisomerase II catalytic site was mapped to the 5' end of the globin gene which contained a nucleosome-free and DNase I-hypersensitive site and additional but minor sites were mapped to the second intron and 3' of the gene to a tissue-specific enhancer. Cleavage sites, mapped in situ by indirect end labeling, were aligned to single-base-pair resolution by comparison to a consensus sequence derived for vertebrate topoisomerase II catalytic sites. In contras

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10

Vassetzky, Y. S., Q. Dang, P. Benedetti, and S. M. Gasser. "Topoisomerase II forms multimers in vitro: effects of metals, beta-glycerophosphate, and phosphorylation of its C-terminal domain." Molecular and Cellular Biology 14, no. 10 (1994): 6962–74. http://dx.doi.org/10.1128/mcb.14.10.6962-6974.1994.

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We present a novel assay for the study of protein-protein interactions involving DNA topoisomerase II. Under various conditions of incubation we observe that topoisomerase II forms complexes at least tetrameric in size, which can be sedimented by centrifugation through glycerol. The multimers are enzymatically active and can be visualized by electron microscopy. Dephosphorylation of topoisomerase II inhibits its multimerization, which can be restored at least partially by rephosphorylation of multiple sites within its 200 C-terminal amino acids by casein kinase II. Truncation of topoisomerase

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11

Vassetzky, Y. S., Q. Dang, P. Benedetti, and S. M. Gasser. "Topoisomerase II forms multimers in vitro: effects of metals, beta-glycerophosphate, and phosphorylation of its C-terminal domain." Molecular and Cellular Biology 14, no. 10 (1994): 6962–74. http://dx.doi.org/10.1128/mcb.14.10.6962.

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We present a novel assay for the study of protein-protein interactions involving DNA topoisomerase II. Under various conditions of incubation we observe that topoisomerase II forms complexes at least tetrameric in size, which can be sedimented by centrifugation through glycerol. The multimers are enzymatically active and can be visualized by electron microscopy. Dephosphorylation of topoisomerase II inhibits its multimerization, which can be restored at least partially by rephosphorylation of multiple sites within its 200 C-terminal amino acids by casein kinase II. Truncation of topoisomerase

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12

Reitman, M., and G. Felsenfeld. "Developmental regulation of topoisomerase II sites and DNase I-hypersensitive sites in the chicken beta-globin locus." Molecular and Cellular Biology 10, no. 6 (1990): 2774–86. http://dx.doi.org/10.1128/mcb.10.6.2774-2786.1990.

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We have mapped DNase I-hypersensitive sites and topoisomerase II (topo II) sites in the chicken beta-globin locus, which contains four globin genes (5'-rho-beta H-beta A-epsilon-3'). In the 65 kilobases (kb) mapped, 12 strong hypersensitive sites were found clustered within the 25-kb region from 10 kb upstream of rho to just downstream of epsilon. The strong sites were grouped into several classes based on their tissue distribution, developmental pattern, and location. (i) One site was present in all cells examined, both erythroid and nonerythroid. (ii) Three sites, located upstream of the rho

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13

Reitman, M., and G. Felsenfeld. "Developmental regulation of topoisomerase II sites and DNase I-hypersensitive sites in the chicken beta-globin locus." Molecular and Cellular Biology 10, no. 6 (1990): 2774–86. http://dx.doi.org/10.1128/mcb.10.6.2774.

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We have mapped DNase I-hypersensitive sites and topoisomerase II (topo II) sites in the chicken beta-globin locus, which contains four globin genes (5'-rho-beta H-beta A-epsilon-3'). In the 65 kilobases (kb) mapped, 12 strong hypersensitive sites were found clustered within the 25-kb region from 10 kb upstream of rho to just downstream of epsilon. The strong sites were grouped into several classes based on their tissue distribution, developmental pattern, and location. (i) One site was present in all cells examined, both erythroid and nonerythroid. (ii) Three sites, located upstream of the rho

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14

Burden, D. A., L. J. Goldsmith, and D. M. Sullivan. "Cell-cycle-dependent phosphorylation and activity of Chinese-hamster ovary topoisomerase II." Biochemical Journal 293, no. 1 (1993): 297–304. http://dx.doi.org/10.1042/bj2930297.

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Cell-cycle-dependent protein levels and phosphorylation of DNA topoisomerase II in relation to its catalytic and cleavage activities were studied in Chinese-hamster ovary cells. Immunoreactive topoisomerase II protein levels were maximal in G2-phase cells, intermediate in S- and M-phase cells, and minimal in a predominantly G1-phase population. When the phosphorylation of topoisomerase II in vivo was corrected for differences in specific radioactivity of intracellular ATP, the apparent phosphorylation of S- and M-phase topoisomerase II was altered significantly. Relative phosphorylation in viv

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15

LONN, Ulf, and Sigrid LONN. "5,6-Dichloro-1-beta-O-ribofuranosylbenzimidazole induces DNA damage by interfering with DNA topoisomerase II." European Journal of Biochemistry 164, no. 3 (1987): 541–45. http://dx.doi.org/10.1111/j.1432-1033.1987.tb11160.x.

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16

Khazeem, Mushtaq M., Ian G. Cowell, Lauren F. Harkin, John W. Casement, and Caroline A. Austin. "Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta." FEBS Letters 594, no. 20 (2020): 3395–405. http://dx.doi.org/10.1002/1873-3468.13904.

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17

Austin, Caroline A., Ian G. Cowell, Mushtaq M. Khazeem, Dawn Lok, and Huei Teng Ng. "TOP2B's contributions to transcription." Biochemical Society Transactions 49, no. 6 (2021): 2483–93. http://dx.doi.org/10.1042/bst20200454.

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Transcription is regulated and mediated by multiprotein complexes in a chromatin context. Transcription causes changes in DNA topology which is modulated by DNA topoisomerases, enzymes that catalyse changes in DNA topology via transient breaking and re-joining of one or both strands of the phosphodiester backbone. Mammals have six DNA topoisomerases, this review focuses on one, DNA topoisomerase II beta (TOP2B). In the absence of TOP2B transcription of many developmentally regulated genes is altered. Long genes seem particularly susceptible to the lack of TOP2B. Biochemical studies of the role

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18

Bukhari, Syed Nasir Abbas, Mohamed Abdelwahab Abdelgawad, Naveed Ahmed, et al. "Synthesis, Characterization, and Biological Evaluation of Meldrum’s Acid Derivatives: Dual Activity and Molecular Docking Study." Pharmaceuticals 16, no. 2 (2023): 281. http://dx.doi.org/10.3390/ph16020281.

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In the presented study, eight novel Meldrum’s acid derivatives containing various vanillic groups were synthesized. Vanillidene Meldrum’s acid compounds were tested against different cancer cell lines and microbes. Out of nine, three showed very good biological activity against E. coli, and HeLa and A549 cell lines. It is shown that the O-alkyl substituted derivatives possessed better antimicrobial and anticancer activities in comparison with the O-acyl ones. The decyl substituted molecule (3i) has the highest activity against E. coli (MIC = 12.4 μM) and cancer cell lines (HeLa, A549, and LS17

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19

Mamontova, Victoria, Barbara Trifault, and Kaspar Burger. "Nono induces Gadd45b to mediate DNA repair." Life Science Alliance 7, no. 8 (2024): e202302555. http://dx.doi.org/10.26508/lsa.202302555.

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RNA-binding proteins are frequently deregulated in cancer and emerge as effectors of the DNA damage response (DDR). The non-POU domain–containing octamer-binding protein NONO/p54nrbis a multifunctional RNA-binding protein that not only modulates the production and processing of mRNA, but also promotes the repair of DNA double-strand breaks (DSBs). Here, we investigate the impact ofNonodeletion in the murine KP (KRasG12D,Trp53−/−) cell–based lung cancer model. We show that the deletion of Nono impairs the response to DNA damage induced by the topoisomerase II inhibitor etoposide or the radiomim

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20

OGAWA, Makoto. "Expression of DNA topoisomerase I and II β in the developing cerebellar plate in rat embryos." Okayama Igakkai Zasshi (Journal of Okayama Medical Association) 111, no. 3-8 (1999): 105–14. http://dx.doi.org/10.4044/joma1947.111.3-8_105.

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21

Ng, Shu-Wing, Yan Liu, and Lowell E. Schnipper. "Cloning and characterization of the 5′-flanking sequence for the human DNA topoisomerase II beta gene." Gene 203, no. 2 (1997): 113–19. http://dx.doi.org/10.1016/s0378-1119(97)00500-3.

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22

Morse-Gaudio, M., and M. S. Risley. "Topoisomerase II expression and VM-26 induction of DNA breaks during spermatogenesis in Xenopus laevis." Journal of Cell Science 107, no. 10 (1994): 2887–98. http://dx.doi.org/10.1242/jcs.107.10.2887.

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The relative content of topoisomerase II (topo II) and the induction of topo-II-mediated DNA damage and cellular abnormalities have been characterized in developing spermatogenic cells of Xenopus laevis to gain an insight into the role of topo II during spermatogenesis. Decatenation assays identified topo II activity in nuclear extracts from spermatocytes and pre-elongate spermatids, but not in extracts from elongate spermatids or sperm. Extracts from early-mid spermatids contained 14% (per cell) of the decatenation activity found in spermatocyte extracts. Immunoblots of SDS extracts from whol

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23

Emmons, M., D. Boulware, D. M. Sullivan, and L. A. Hazlehurst. "Topoisomerase II beta levels are a determinant of melphalan-induced DNA crosslinks and sensitivity to cell death." Biochemical Pharmacology 72, no. 1 (2006): 11–18. http://dx.doi.org/10.1016/j.bcp.2006.03.017.

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24

Bernstein, Carol. "DNA Methylation and Establishing Memory." Epigenetics Insights 15 (January 2022): 251686572110724. http://dx.doi.org/10.1177/25168657211072499.

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A single event can cause a life-long memory. Memories physically reside in neurons, and changes in neuronal gene expression are considered to be central to memory. Early models proposed that specific DNA methylations of cytosines in neuronal DNA encode memories in a stable biochemical form. This review describes recent research that elucidates the molecular mechanisms used by the mammalian brain to form DNA methylcytosine encoded memories. For example, neuron activation initiates cytosine demethylation by stimulating DNA topoisomerase II beta (TOP2B) protein to make a temporary DNA double-stra

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25

Cybulski, Marcin, Katarzyna Sidoryk, Magdalena Zaremba-Czogalla, et al. "The Conjugates of Indolo[2,3-b]quinoline as Anti-Pancreatic Cancer Agents: Design, Synthesis, Molecular Docking and Biological Evaluations." International Journal of Molecular Sciences 25, no. 5 (2024): 2573. http://dx.doi.org/10.3390/ijms25052573.

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New amide conjugates of hydroxycinnamic acids (HCAs) and the known antineoplastic 5,11-dimethyl-5H-indolo[2,3-b]quinoline (DiMIQ), an analog of the natural alkaloid neocryptolepine, were synthesized and tested in vitro for anticancer activity. The compound 9-[((2-hydroxy)cinnamoyl)amino]-5,11-dimethyl-5H-indolo[2,3-b]quinoline (2), which contains the ortho-coumaric acid fragment, demonstrated dose-dependent effectiveness against both normal BxPC-3 and metastatic AsPC-1 pancreatic cancer cells. The IC50 values for AsPC-1 and BxPC-3 were 336.5 nM and 347.5 nM, respectively, with a selectivity in

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Tomkins, C. E., S. N. Edwards, and A. M. Tolkovsky. "Apoptosis is induced in post-mitotic rat sympathetic neurons by arabinosides and topoisomerase II inhibitors in the presence of NGF." Journal of Cell Science 107, no. 6 (1994): 1499–507. http://dx.doi.org/10.1242/jcs.107.6.1499.

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Sympathetic neurons depend on nerve growth factor (NGF) for their survival and die by apoptosis when NGF is withdrawn, despite their post-mitotic state. Martin et al. (1990, J. Neurosci. 10, 184–193) showed that cytosine arabinoside, but no other arabinofuranosyl nucleoside, could induce cell death in the presence of NGF and they suggested that it may block a critical step in the NGF-signalling pathway. We show that cytosine arabinoside is not the only nucleoside capable of inducing apoptosis in sympathetic neurons in the presence of NGF. In newly isolated neurons from P0 rat pups cultured in

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27

Broyles, S. S., and B. Moss. "Sedimentation of an RNA polymerase complex from vaccinia virus that specifically initiates and terminates transcription." Molecular and Cellular Biology 7, no. 1 (1987): 7–14. http://dx.doi.org/10.1128/mcb.7.1.7-14.1987.

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A high-molecular-weight protein complex that is capable of accurate transcription initiation and termination of vaccinia virus early genes without additional factors was demonstrated. The complex was solubilized by disruption of purified virions, freed of DNA by passage through a DEAE-cellulose column, and isolated by glycerol gradient sedimentation. All detectable RNA polymerase activity was associated with the transcription complex, whereas the majority of enzymes released from virus cores including mRNA (nucleoside-2'-O)methyltransferase, poly(A) polymerase, topoisomerase, nucleoside tripho

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Broyles, S. S., and B. Moss. "Sedimentation of an RNA polymerase complex from vaccinia virus that specifically initiates and terminates transcription." Molecular and Cellular Biology 7, no. 1 (1987): 7–14. http://dx.doi.org/10.1128/mcb.7.1.7.

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A high-molecular-weight protein complex that is capable of accurate transcription initiation and termination of vaccinia virus early genes without additional factors was demonstrated. The complex was solubilized by disruption of purified virions, freed of DNA by passage through a DEAE-cellulose column, and isolated by glycerol gradient sedimentation. All detectable RNA polymerase activity was associated with the transcription complex, whereas the majority of enzymes released from virus cores including mRNA (nucleoside-2'-O)methyltransferase, poly(A) polymerase, topoisomerase, nucleoside tripho

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29

Harker, W. Graydon, D. Lynn Slade, Fred H. Drake, and Ryan L. Parr. "Mitoxantrone resistance in HL-60 leukemia cells: reduced nuclear topoisomerase II catalytic activity and drug-induced DNA cleavage in association with reduced expression of the topoisomerase II .beta. isoform." Biochemistry 30, no. 41 (1991): 9953–61. http://dx.doi.org/10.1021/bi00105a020.

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30

Calderón-Montaño, José Manuel, Estefanía Burgos-Morón, Manuel Luis Orta, et al. "Alpha, beta-unsaturated lactones 2-furanone and 2-pyrone induce cellular DNA damage, formation of topoisomerase I- and II-DNA complexes and cancer cell death." Toxicology Letters 222, no. 1 (2013): 64–71. http://dx.doi.org/10.1016/j.toxlet.2013.07.007.

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31

Kuwana, M., T. A. Medsger, and T. M. Wright. "Highly restricted TCR-alpha beta usage by autoreactive human T cell clones specific for DNA topoisomerase I: recognition of an immunodominant epitope." Journal of Immunology 158, no. 1 (1997): 485–91. http://dx.doi.org/10.4049/jimmunol.158.1.485.

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Abstract Autoantibody responses to DNA topoisomerase I (Topo I) are highly specific to patients with systemic sclerosis (SSc). We recently demonstrated that Topo I-specific T cells are components of the T cell repertoire of patients with SSc and healthy individuals. These autoreactive T cells were essential for the Ag-specific activation of B cells resulting in anti-Topo I Ab production in vitro and therefore are believed to play a central role in autoantibody production. To characterize the Topo I-specific T cell, 15 T cell clones reactive with Topo I were generated from two patients with SSc

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32

Fleenor, DE, and RE Kaufman. "Characterization of the DNase I hypersensitive site 3' of the human beta globin gene domain." Blood 81, no. 10 (1993): 2781–90. http://dx.doi.org/10.1182/blood.v81.10.2781.2781.

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Abstract The members of the human beta globin gene family are flanked by strong DNase I hypersensitive sites. The collection of sites 5' to the epsilon globin gene is able to confer high levels of expression of linked globin genes, but a function has not been assigned to the site 3' to the beta globin gene (3'HS1). Our analysis of this DNase I super hypersensitive site shows that the region is composed of multiple DNase I sites. By examination of the DNA sequence, we have determined that the region is very A/T-rich and contains topoisomerase II recognition sequences, as well as several consens

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Fleenor, DE, and RE Kaufman. "Characterization of the DNase I hypersensitive site 3' of the human beta globin gene domain." Blood 81, no. 10 (1993): 2781–90. http://dx.doi.org/10.1182/blood.v81.10.2781.bloodjournal81102781.

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The members of the human beta globin gene family are flanked by strong DNase I hypersensitive sites. The collection of sites 5' to the epsilon globin gene is able to confer high levels of expression of linked globin genes, but a function has not been assigned to the site 3' to the beta globin gene (3'HS1). Our analysis of this DNase I super hypersensitive site shows that the region is composed of multiple DNase I sites. By examination of the DNA sequence, we have determined that the region is very A/T-rich and contains topoisomerase II recognition sequences, as well as several consensus bindin

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34

Baumann, H., and G. Strassmann. "Suramin inhibits the stimulation of acute phase plasma protein genes by IL-6-type cytokines in rat hepatoma cells." Journal of Immunology 151, no. 3 (1993): 1456–62. http://dx.doi.org/10.4049/jimmunol.151.3.1456.

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Abstract The cytokines, IL-1 beta, TNF-alpha, IL-6, IL-11, leukemia inhibitory factor, and ciliary neurotrophic factor, stimulate the expression of specific sets of acute phase plasma proteins in the rat hepatoma H-35 cell line. In this paper, the experimental drug suramin is shown to inhibit in a dose-dependent fashion the cell response to these cytokines, with the exception of TNF-alpha. The action of the IL-6-type cytokines is more sensitive to suramin inhibition that that of IL-1 beta. The effect of suramin is detectable within 30 min at the level of gene transcription and appears to be me

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Voggu, Ramakrishna, Arundhati Karmakar, Venkat Swamy Puli та ін. "Design, Synthesis, Molecular Docking Study and Biological Evaluation of Novel γ-Carboline Derivatives of Latrepirdine (Dimebon) as Potent Anticancer Agents". Molecules 28, № 13 (2023): 4965. http://dx.doi.org/10.3390/molecules28134965.

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A series of novel γ-Carboline derivatives were designed and synthesized using the Suzuki coupling reaction to identify the leads for the activity against cancer. Interestingly, these compounds were tested for their anticancer activity against the cell lines, particularly human cancer cell lines MCF7 (breast), A549 (lung), SiHa (cervix), and Colo-205 (colon). Most of the γ-Carboline derivatives showed potent inhibitory activity in four cancer cell lines, according to in vitro anticancer activity screening. Two compounds, specifically LP-14 and LP-15, showed superior activity in cancer cell line

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36

Jazwinski, S. M. "Participation of ATP in the binding of a yeast replicative complex to DNA." Biochemical Journal 246, no. 1 (1987): 213–19. http://dx.doi.org/10.1042/bj2460213.

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The activity that replicates yeast DNA in vitro can be isolated from cells of the budding yeast Saccharomyces in a high-Mr (approximately 2 × 10(6] form. Several lines of evidence indicate that this fraction contains a multiprotein replicative complex. A functional assay has been developed for the analysis of the interaction of the replicating activity with DNA. Binding of the activity required Mg2+, but did not require the addition of ATP or the other ribo- or deoxynucleoside triphosphates. However, the ATP analogues adenosine 5′-[gamma-thio]triphosphate and adenosine 5′-[beta gamma-imido]tri

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Burden, D. Andrew, and Daniel M. Sullivan. "Phosphorylation of the .alpha.- and .beta.-Isoforms of DNA Topoisomerase II Is Qualitatively Different in Interphase and Mitosis in Chinese Hamster Ovary Cells." Biochemistry 33, no. 49 (1994): 14651–55. http://dx.doi.org/10.1021/bi00253a001.

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38

Wang, Zhe Qing, Yao Haur Kuo, Dora Schnur, et al. "Antitumor agents. 113. New 4.beta.-arylamino derivatives of 4'-O-demethylepipodophyllotoxin and related compounds as potent inhibitors of human DNA topoisomerase II." Journal of Medicinal Chemistry 33, no. 9 (1990): 2660–66. http://dx.doi.org/10.1021/jm00171a050.

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Hu, Hong, Su Ying Liu, Yung Chi Cheng, Kuo Hsiung Lee, and Zhe Qing Wang. "Antitumor agents. 123. Synthesis and human DNA topoisomerase II inhibitory activity of 2'-chloro derivatives of etoposide and 4.beta.-(arylamino)-4'-O-demethylpodophyllotoxins." Journal of Medicinal Chemistry 35, no. 5 (1992): 866–71. http://dx.doi.org/10.1021/jm00083a009.

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Kimura, K., N. Nozaki, M. Saijo, A. Kikuchi, M. Ui, and T. Enomoto. "Identification of the nature of modification that causes the shift of DNA topoisomerase II beta to apparent higher molecular weight forms in the M phase." Journal of Biological Chemistry 269, no. 40 (1994): 24523–26. http://dx.doi.org/10.1016/s0021-9258(17)31419-9.

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Wang, Zhe-Qing, Hong Hu, Hong-Xin Chen, Yung-Chi Cheng, and Kuo Hsiung Lee. "Antitumor agents. 124. New 4.beta.-substituted aniline derivatives of 6,7-O,O-demethylene-4'-O-demethylpodophyllotoxin and related compounds as potent inhibitors of human DNA topoisomerase II." Journal of Medicinal Chemistry 35, no. 5 (1992): 871–77. http://dx.doi.org/10.1021/jm00083a010.

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Charron, Martin, and Ronald Hancock. "DNA topoisomerase II is required for formation of mitotic chromosomes in Chinese hamster ovary cells: studies using the inhibitor 4'-demethylepipodophyllotoxin 9-(4,6-O-thenylidene-.beta.-D-glucopyranoside)." Biochemistry 29, no. 41 (1990): 9531–37. http://dx.doi.org/10.1021/bi00493a006.

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Ibrahim, Merna M., and Li Cai. "Top2b-Regulated Genes and Pathways Linked to Retinal Homeostasis and Degeneration." Cells 14, no. 12 (2025): 887. https://doi.org/10.3390/cells14120887.

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Retinal homeostasis and degeneration are significant contributors to global vision loss, with retinal health primarily assessed by the count and function of photoreceptor cells, the most abundant cells in the retina. Genomic studies have identified topoisomerase II beta (Top2b), an enzyme that untangles DNA supercoils to facilitate gene expression, as a critical transcriptional regulator for retinal health. This review aims to uncover and categorize genes linked to Top2b that are dynamically expressed during retinal degeneration, revealing shared and overlooked regulatory pathways. RNA sequenc

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Renzi, L., M. S. Gersch, M. S. Campbell, L. Wu, S. A. Osmani, and G. J. Gorbsky. "MPM-2 antibody-reactive phosphorylations can be created in detergent-extracted cells by kinetochore-bound and soluble kinases." Journal of Cell Science 110, no. 17 (1997): 2013–25. http://dx.doi.org/10.1242/jcs.110.17.2013.

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The MPM-2 antibody labels mitosis-specific and cell cycle-regulated phosphoproteins. The major phosphoproteins of mitotic chromosomes recognized by the MPM-2 antibody are DNA topoisomerase II (topoII) alpha and beta. In immunofluorescence studies of PtK1 cytoskeletons, prepared by detergent lysis in the presence of potent phosphatase inhibitors, the MPM-2 antibody labels phosphoproteins found at kinetochores, chromosome arms, midbody and spindle poles of mitotic cells. In cells extracted without phosphatase inhibitors, labeling of the MPM-2 antibodies at kinetochores is greatly diminished. How

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Zielinski, Rafal J., Krzysztof Grela, Roberto Cardenas-Zuniga та ін. "Abstract LB180: Non-cardiotoxic properties of annamycin, a clinically evaluated anthracycline and potent topoisomerase 2β poison". Cancer Research 84, № 7_Supplement (2024): LB180. http://dx.doi.org/10.1158/1538-7445.am2024-lb180.

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Abstract Introduction. Cardiotoxicity is an important side effect limiting the clinical use of all anthracyclines, including doxorubicin (DOX). DOX targets topoisomerase II alpha (Top2α) and beta (Top2β), two enzymes that regulate DNA topology. Top2β is present in cardiomyocytes and has been proposed as a major contributor to DOX-induced cardiotoxicity. Annamycin (ANN), a novel clinically evaluated DOX analog, displays suppressed or no cardiotoxic properties in preclinical in vivo experiments. The objective of this study was to directly assess and compare the potency of DOX and ANN against Top

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Komatsu, M., E. Lamoyi, and R. G. Mage. "Genomic DNA encoding rabbit T cell receptor beta-chains: isotypes and allotypes of C beta." Journal of Immunology 138, no. 5 (1987): 1621–26. http://dx.doi.org/10.4049/jimmunol.138.5.1621.

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Abstract We have discovered sequence differences in DNA encoding the first exon of rabbit T cell receptor beta-chains from unrelated rabbits that probably reflect allelic C beta 1 allotypes. Rabbit I was from a colony bred to maintain the K1-expression mutation Basilea, and rabbit II was from a colony bred to maintain the K1b9 allotype. Genomic DNA from rabbits I and II also exhibit restriction fragment length polymorphism of C beta on Southern blots. In addition, several different restriction enzyme digests of DNA from rabbit I give three bands, whereas DNA from rabbit II gives two when probe

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Celada, A., S. McKercher, and R. A. Maki. "Repression of major histocompatibility complex IA expression by glucocorticoids: the glucocorticoid receptor inhibits the DNA binding of the X box DNA binding protein." Journal of Experimental Medicine 177, no. 3 (1993): 691–98. http://dx.doi.org/10.1084/jem.177.3.691.

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Glucocorticoids are effective repressors of major histocompatibility complex (MHC) class II gene expression. The repression occurs in B cells, which constitutively express MHC class II, as well as in macrophages, which only express MHC class II after the cells are treated with interferon gamma. For the MHC class II gene IA beta, this negative regulation has been linked to the X box DNA sequence, located with the IA beta promoter. The addition of the glucocorticoid receptor was shown to inhibit the DNA binding of the X box DNA binding protein to the X box. The DNA binding of two other DNA bindi

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Ullah, Najeeb, Shan Zeb, Fozia, et al. "Bioassay’s Directed Isolation-Structure Elucidation and Molecular Docking of Triterpenes from Persea duthiei against Biologically Important Microbial Proteins." Evidence-Based Complementary and Alternative Medicine 2022 (May 27, 2022): 1–12. http://dx.doi.org/10.1155/2022/3839271.

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The research work presented in this study is mainly concerned with the bioactivity-directed phytochemical and biological evaluation of Persea duthiei. Persea duthiei is a typical medicinal plant used to treat a variety of ailments such as asthma, edema, and bronchitis. Ethyl acetate, n-hexane, n-butanol, and compounds that are soluble in water were used to examine the antibacterial as well as antifungal capacities of the plant. The antibacterial activity of the soluble parts of ethyl acetate and n-hexane against Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Bacillus subtilis w

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Xu, Y. X., J. Pitcovski, L. Peterson, et al. "Isolation and characterization of three class II MHC genomic clones from the chicken." Journal of Immunology 142, no. 6 (1989): 2122–32. http://dx.doi.org/10.4049/jimmunol.142.6.2122.

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Abstract A genomic library was constructed from sperm DNA from an individual of the inbred chicken line G-B2, MHC haplotype B6. The library was screened with a chicken class II probe (beta 2 exon specific) and three MHC class II beta chain genomic clones were isolated. The restriction maps of the three clones showed that each of the three clones was unique. The position of the beta chain sequence was located in each of the three genomic clones by Southern blot hybridization. Subclones containing the beta chain gene were produced from each of the genomic clones and the orientation of the leader

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Klohe, E. P., R. Watts, M. Bahl, et al. "Analysis of the molecular specificities of anti-class II monoclonal antibodies by using L cell transfectants expressing HLA class II molecules." Journal of Immunology 141, no. 6 (1988): 2158–64. http://dx.doi.org/10.4049/jimmunol.141.6.2158.

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Abstract Expressible HLA class II alpha- and beta-chain cDNA were used for DNA-mediated gene transfer to produce L cell transfectants expressing single types of human class II molecules. Cloned transfectants expressing nine different class II molecules were isolated: DR alpha: DR1 beta I, DR alpha: DR4 beta I, DR alpha: DR5 beta I, DR alpha: DR5 beta III (DRw52), DR alpha: DR7 beta I, DR alpha: DR4/7 beta IV (DRw53), DQ7 alpha: DQw2 beta, DQ7 alpha: DQw3 beta, and DPw4 alpha: DPw4 beta. These class II-expressing transfectants were used to analyze by flow cytometry the molecular specificities o

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