Relevant bibliographies by topics / DNBSEQ-G400

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Academic literature on the topic 'DNBSEQ-G400'

Author: Grafiati
Published: 4 June 2025
Last updated: 1 August 2025

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Journal articles on the topic "DNBSEQ-G400"

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Sun, Xiaohuan, Yue-Hua Hu, Jingjing Wang, et al. "Efficient and stable metabarcoding sequencing data using a DNBSEQ-G400 sequencer validated by comprehensive community analyses." Gigabyte 2021 (March 19, 2021): 1–15. http://dx.doi.org/10.46471/gigabyte.16.

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Metabarcoding is a widely used method for fast characterization of microbial communities in complex environmental samples. However, the selction of sequencing platform can have a noticeable effect on the estimated community composition. Here, we evaluated the metabarcoding performance of a DNBSEQ-G400 sequencer developed by MGI Tech using 16S and internal transcribed spacer (ITS) markers to investigate bacterial and fungal mock communities, as well as the ITS2 marker to investigate the fungal community of 1144 soil samples, with additional technical replicates. We show that highly accurate seq
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Konanov, Dmitry N., Vera Y. Tereshchuk, Ignat V. Sonets, et al. "Analysis of Software Read Cross-Contamination in DNBSEQ Data." Biology 14, no. 6 (2025): 670. https://doi.org/10.3390/biology14060670.

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DNA nanoball sequencing (DNBSEQ) is one of the most rapidly developing sequencing technologies and is widely applied in genomic and transcriptomic investigations. Recently, a new PE300 sequencing option primarily recommended for amplicon analysis was released for DNBSEQ-G99 and G400 devices. Given their unprecedentedly high data yield per flow cell, the new PE300 kits could be a great choice for various sequencing tasks, but we found that combining different types of DNA libraries in a single run could lead to undesired artifacts in the data. In this study, we investigate the occasional read c
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Makarova, M. V., M. V. Nemtsova, M. S. Belenikin, et al. "The diagnosis of hereditary cancer syndromes with atypical manifestation: clinical cases." Malignant tumours 13, no. 4 (2023): 93–100. http://dx.doi.org/10.18027/2224-5057-2023-13-4-93-100.

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Background: Germinal pathogenic variants are the cause of the development of hereditary cancer syndromes (HCS). Various genetic tests are used for HCS detect, from the «frequent» mutations of one or several genes analysis to the full-length gene sequence, next-generation sequencing (NGS) based panel, whole exome (WES) or whole genome sequencing (WGS).There are some HCS cases with atypical clinical manifestations and the family history does not allow one to suspect a specific HCS and limit oneself to the study of only one or a few genes. Conducting research using NGS to assess the selected samp
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Pavlova, Anna, Vera Belova, Robert Afasizhev, Irina Bulusheva, Denis Rebrikov, and Dmitriy Korostin. "Runcer-Necromancer: a method to rescue data from an interrupted run on MGISEQ-2000." F1000Research 10 (January 14, 2021): 22. http://dx.doi.org/10.12688/f1000research.27763.1.

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During the sequencing process, problems can occur with any device, including the MGISEQ-2000 (DNBSEQ-G400) platform. We encountered a power outage that resulted in a temporary shutdown of a sequencer in the middle of the run. Since barcode reading in MGISEQ-2000 takes place at the end of the run, it was impossible to use non-demultiplexed raw data. We decided to completely use up the same cartridge with reagents and flow cell loaded with DNB and started a new run in a shortened custom mode. We figured out how the MGISEQ-2000 converts preliminary data in .cal format into .fastq files and wrote
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Pavlova, Anna, Vera Belova, Robert Afasizhev, Irina Bulusheva, Denis Rebrikov, and Dmitriy Korostin. "Runcer-Necromancer: a method to rescue data from an interrupted run on MGISEQ-2000." F1000Research 10 (February 14, 2022): 22. http://dx.doi.org/10.12688/f1000research.27763.2.

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During the sequencing process, problems can occur with any device, including the MGISEQ-2000 (DNBSEQ-G400) platform. We encountered a power outage that resulted in a temporary shutdown of a sequencer in the middle of the run. Since barcode reading in MGISEQ-2000 takes place at the end of the run, it was impossible to use non-demultiplexed raw data. We decided to completely use up the same cartridge with reagents and flow cell loaded with DNB and started a new run in a shortened custom mode. We figured out how the MGISEQ-2000 converts preliminary data in .cal format into .fastq files and wrote
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6

Haars, Jonathan, Navaneethan Palanisamy, Frans Wallin, et al. "Prevalence of SARS-CoV-2 Omicron Sublineages and Spike Protein Mutations Conferring Resistance against Monoclonal Antibodies in a Swedish Cohort during 2022–2023." Microorganisms 11, no. 10 (2023): 2417. http://dx.doi.org/10.3390/microorganisms11102417.

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Monoclonal antibodies (mAbs) are an important treatment option for COVID-19 caused by SARS-CoV-2, especially in immunosuppressed patients. However, this treatment option can become ineffective due to mutations in the SARS-CoV-2 genome, mainly in the receptor binding domain (RBD) of the spike (S) protein. In the present study, 7950 SARS-CoV-2 positive samples from the Uppsala and Örebro regions of central Sweden, collected between March 2022 and May 2023, were whole-genome sequenced using amplicon-based sequencing methods on Oxford Nanopore GridION, Illumina MiSeq, Illumina HiSeq, or MGI DNBSEQ
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Ульянова, Татьяна, Индира Бейшова, Диляра Гриценко та ін. "РЕСЕКВЕНИРОВАНИЕ ПОЛНОГО ГЕНОМА КРУПНОГО РОГАТОГО СКОТА КАЗАХСКОЙ БЕЛОГОЛОВОЙ ПОРОДЫ". Ġylym ža̋ne bìlìm 2, № 3(76) (2024): 130–40. https://doi.org/10.52578/2305-9397-2024-3-2-130-140.

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В статье представлена характеристика первой полученной последовательности генома крупного рогатого скота казахской белоголовой породы в Казахстане. Целью работы было провести ресеквенирование крупного рогатого скота казахской белоголовой породы, разводимой в Республике Казахстан. Были собраны образцы цельной крови и волосяных луковиц наиболее типичных эталонных представителей казахской белоголовой породы. Для проведения полногеномного ресеквенирования выделена ДНК из образцов отобранного биоматериала с использованием коммерческих наборов «ДНК Экстран-2» (ООО «Синтол», РФ), «PureLink Genomic DN
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Dolotkazin, D. R., D. A. Averinskaya, E. N. Knyazev, et al. "Assessment of miR-21-5p, miR-451a, and miR-144-3p level in urine in differential diagnosis of localized prostate cancer." Cancer Urology 20, no. 1 (2024): 36–43. http://dx.doi.org/10.17650/1726-9776-2024-20-1-36-43.

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Background. Limited sensitivity and specificity of existing prostate cancer (PCa) diagnosis methods drive the search for new markers. A number of studies has demonstrated the potential for measuring expression of certain microRNAs in urine.Aim. To evaluate the diagnostic potential of measuring microRNA expression in urine in PCa.Materials and methods. A collection of urine sediment samples from 19 patients with benign prostatic hyperplasia and 44 patients with PCa was analyzed. RNA was isolated using the miRNEasy Serum/Plasma Kit. 16 µL of RNA isolated from each sample were converted into cDNA
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Shimansky, Valentin, Oleg Popov, Alexander Kel, et al. "Analysis of the Expression Profile in COVID-19 Patients in the Russian Population Considering Disease Severity, Mortality, and Cytokine Storm." Biomedicines 13, no. 4 (2025): 863. https://doi.org/10.3390/biomedicines13040863.

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Background/Objectives: The COVID-19 pandemic has posed a significant challenge to global healthcare systems and has prompted a need for a better understanding of the molecular mechanisms underlying SARS-CoV-2 infection. This study aims to analyze differential gene expression in COVID-19 patients to identify regulatory genes influencing key pathways involved in disease progression. Methods: We conducted a transcriptomic analysis of patients admitted to the Infectious Disease Department of City Hospital No. 40, confirmed with SARS-CoV-2 via PCR. The study received ethical approval (protocol No.
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10

Gervas, P. A., A. Yu Molokov, N. N. Babyshkina, et al. "Whole exome sequencing: the search for mutations associated with hereditary breast cancer in ethnic groups of Siberia." Siberian journal of oncology 23, no. 5 (2024): 35–46. http://dx.doi.org/10.21294/1814-4861-2024-23-5-35-46.

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Hereditary breast cancer (HBC) is a heterogeneous disease caused by mutations in genes characterized by ethnic specifcity. The clinical heterogeneity of this disease signifcantly complicates its diagnosis. The use of high-throughput sequencing is one of the approaches that allow the search for genes and their variants associated with the development of HBC. The purpose of the study was to search for new genes associated with HBC in the understudied ethnic groups of Siberia by using whole exome sequencing (WES).Material and Methods. WES was performed on a cohort of 16 probands with BC (Tuvan, Y
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Conference papers on the topic "DNBSEQ-G400"

1

Jácomo, Rafael Henriques, Anderson Coqueiro dos Santos, Pedro Góes Mesquita, et al. "Migração do sequenciamento de um exoma clínico previamente validado para a plataforma MGI DNBSEQ-G400: dados NGS de alta qualidade e elevada concordância com resultados esperados." In Resumos do 56º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2024. https://doi.org/10.5327/1516-3180.142s1.12872.

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Objetivo: Esta validação visou transferir o processamento de um exoma clínico de 4700 genes relacionados a desordens hereditárias para o sequenciador DNBSEQ-G400 (MGI Tech Co.), avaliando a quantidade e a qualidade dos dados NGS gerados e a concordância na chamada de variantes, em comparação com o procedimento previamente validado no NextSeq-500 (Illumina, Inc.). Método: O DNA da saliva de 12 voluntários foi submetido ao preparo de bibliotecas e à captura dos genes contidos no Inherited Disease Panel, utilizando o xGEN DNA Library Prep and Hybridization Capture (Integrated DNA Technologies, In
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Jácomo, Rafael Henriques, Anderson Coqueiro dos Santos, Nara Diniz Soares Pessoa, et al. "Transferência do sequenciamento de um painel multicâncer hereditário previamente validado para a plataforma MGI DNBSEQ-G400: dados NGS de alta qualidade e elevada concordância com resultados esperados." In Resumos do 56º Congresso Brasileiro de Patologia Clínica/Medicina Laboratorial. Zeppelini Editorial e Comunicação, 2024. https://doi.org/10.5327/1516-3180.142s1.12595.

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Objetivo: Esta validação visou transferir o processamento de um painel de 221 genes relacionados ao câncer germinativo para o sequenciador DNBSEQ-G400 (MGI Tech Co), avaliando a quantidade e a qualidade dos dados NGS gerados e a concordância na chamada de variantes, em comparação com o procedimento previamente validado no NextSeq-500 (Illumina, Inc). Método: O DNA do sangue de 12 voluntários adultos foi submetido ao preparo de bibliotecas e à captura do painel multicâncer foco desta validação, utilizando o xGEN DNA Library Prep and Hybridization Capture (Integrated DNA Technologies, Inc). O co
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