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Journal articles on the topic 'Donor strains'

Author: Grafiati

Published: 4 June 2021

Last updated: 19 February 2022

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1

Laskus, Tomasz, Lian-Fu Wang, Marek Radkowski, Hugo Vargas, Marek Nowicki, Jeffrey Wilkinson, and Jorge Rakela. "Exposure of Hepatitis C Virus (HCV) RNA-Positive Recipients to HCV RNA-Positive Blood Donors Results in Rapid Predominance of a Single Donor Strain and Exclusion and/or Suppression of the Recipient Strain." Journal of Virology 75, no. 5 (March 1, 2001): 2059–66. http://dx.doi.org/10.1128/jvi.75.5.2059-2066.2001.

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ABSTRACT We have analyzed three cases of hepatitis C virus (HCV)-infected recipients who received blood from HCV-infected donors. Two recipients were exposed to two different HCV RNA-positive donors, and one was exposed to a single donor. All parental genomes from the actual infecting units of blood and the recipients were defined, and their presence in the follow-up serum samples was determined using sensitive strain-specific assays. The strain from one of the donors was found to predominate in all recipients' serum samples collected throughout the follow-up period of 10 to 30 months. In two

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2

Zhu, Raymond, Robert Pesun, Caroline Pampolina, Elke Jackson, George Bowden, Teresa Zelinski, and Archibald McNicol. "A role for immunoglobulin G in donor-specific Streptococcus sanguis-induced platelet aggregation." Thrombosis and Haemostasis 95, no. 02 (2006): 288–93. http://dx.doi.org/10.1160/th05-07-0491.

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SummaryThere is increasing evidence fora relationship between bacterial infections and several cardiovascular disorders. Although the precise mechanism(s) underlying this association is unknown, the direct activation of platelets by bacteria is one possibility. Individual strains of S. sanguis activate platelets in a non-uniform, donor-dependent manner. In the current study, platelet aggregation profiles were obtained for fourteen donors in response to four strains of S. sanguis (2017–78, 133–79, SK112, SK108a) and one of S. gordonii (SK8). The platelets from all donors responded to strains 20

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3

Ochi, Kentaro, Maho Tokuda, Kosuke Yanagiya, Chiho Suzuki-Minakuchi, Hideaki Nojiri, Masahiro Yuki, Moriya Ohkuma, Kazuhide Kimbara, and Masaki Shintani. "Oxygen concentration affects frequency and range of transconjugants for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1." Bioscience, Biotechnology, and Biochemistry 85, no. 4 (December 26, 2020): 1005–15. http://dx.doi.org/10.1093/bbb/zbaa118.

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ABSTRACT The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. To compare the tr

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4

Shi, Xian’ai, Hang Wang, Hao Wang, Zhiming Wang, and Chun Meng. "Transferring chromosome DNA fragments from multiple donor cells into a host strain for yeast strain improvement." Canadian Journal of Microbiology 58, no. 6 (June 2012): 760–66. http://dx.doi.org/10.1139/w2012-036.

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Based on a common biological phenomenon — homologous recombination — a novel method was developed by transferring chromosome DNA fragments extracted from multiple donor cells into a host strain. Through this method of transferring DNA fragments, foreign DNA fragments are introduced into one host cell and multiple positive traits from multiple strains may be integrated into the host strain. We first confirmed its feasibility in both prokaryotic and eukaryotic cells by selecting reverse mutants to prototrophy from auxotrophic strains through receiving chromosomal DNA fragments of wild-type paren

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5

Thomas, D. John I., J. Alun W. Morgan, John M. Whipps, and Jon R. Saunders. "Plasmid Transfer between the Bacillus thuringiensis Subspecies kurstaki andtenebrionis in Laboratory Culture and Soil and in Lepidopteran and Coleopteran Larvae." Applied and Environmental Microbiology 66, no. 1 (January 1, 2000): 118–24. http://dx.doi.org/10.1128/aem.66.1.118-124.2000.

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ABSTRACT Plasmid transfer between Bacillus thuringiensis subsp.kurstaki HD1 and B. thuringiensis subsp.tenebrionis donor strains and a streptomycin-resistantB. thuringiensis subsp. kurstaki recipient was studied under environmentally relevant laboratory conditions in vitro, in soil, and in insects. Plasmid transfer was detected in vitro at temperatures of 5 to 37°C, at pH 5.9 to 9.0, and at water activities of 0.965 to 0.995, and the highest transfer ratios (up to 10−1 transconjugant/donor) were detected within 4 h. In contrast, no plasmid transfer was detected in nonsterile soil, and rapid fo

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6

Signorini, Lucia, Maria Dolci, Evaldo Favi, Caterina Colico, Mariano Ferraresso, Rosalia Ticozzi, Giuseppe Basile, Pasquale Ferrante, and Serena Delbue. "Viral Genomic Characterization and Replication Pattern of Human Polyomaviruses in Kidney Transplant Recipients." Viruses 12, no. 11 (November 9, 2020): 1280. http://dx.doi.org/10.3390/v12111280.

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Human Polyomavirus (HPyV) infections are common, ranging from 60% to 100%. In kidney transplant (KTx) recipients, HPyVs have been associated with allograft nephropathy, progressive multifocal leukoencephalopathy, and skin cancer. Whether such complications are caused by viral reactivation or primary infection transmitted by the donor remains debated. This study aimed to investigate the replication pattern and genomic characterization of BK Polyomavirus (BKPyV), JC Polyomavirus (JCPyV), and Merkel Cell Polyomavirus (MCPyV) infections in KTx. Urine samples from 57 KTx donor/recipient pairs were

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7

Weil, Michael M., Barry W. Brown, and Dan M. Serachitopol. "Genotype Selection to Rapidly Breed Congenic Strains." Genetics 146, no. 3 (July 1, 1997): 1061–69. http://dx.doi.org/10.1093/genetics/146.3.1061.

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Congenic strains can now be constructed guided by the transmission of DNA markers. This allows not only selection for transmission of a desired, donor-derived differential region but also selection against the transmission of unwanted donor origin genomic material. The additional selection capacity should allow congenic strains to be produced in fewer generations than is possible with random backcrosses. Here, we consider modifications of a standard backcross breeding scheme to produce congenic mice by the inclusion of genotype-based selective breeding strategies. Simulation is used to evaluat

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8

Blesa, Alba, Ignacio Baquedano, Sandra González-de la Fuente, Mario Mencía, and José Berenguer. "Integrative and Conjugative Element ICETh1 Functions as a Pangenomic DNA Capture Module in Thermus thermophilus." Microorganisms 8, no. 12 (December 21, 2020): 2051. http://dx.doi.org/10.3390/microorganisms8122051.

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Transjugation is an unconventional conjugation mechanism in Thermus thermophilus (Tth) that involves the active participation of both mating partners, encompassing a DNA secretion system (DSS) in the donor and an active natural competence apparatus (NCA) in the recipient cells. DSS is encoded within an integrative and conjugative element (ICETh1) in the strain Tth HB27, whereas the NCA is constitutively expressed in both mates. Previous experiments suggested the presence of multiple origins of transfer along the genome, which could generate genomic mosaicity among the progeny. Here, we designe

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9

Ferrières, Lionel, Gaëlle Hémery, Toan Nham, Anne-Marie Guérout, Didier Mazel, Christophe Beloin, and Jean-Marc Ghigo. "Silent Mischief: Bacteriophage Mu Insertions Contaminate Products of Escherichia coli Random Mutagenesis Performed Using Suicidal Transposon Delivery Plasmids Mobilized by Broad-Host-Range RP4 Conjugative Machinery." Journal of Bacteriology 192, no. 24 (October 8, 2010): 6418–27. http://dx.doi.org/10.1128/jb.00621-10.

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ABSTRACT Random transposon mutagenesis is the strategy of choice for associating a phenotype with its unknown genetic determinants. It is generally performed by mobilization of a conditionally replicating vector delivering transposons to recipient cells using broad-host-range RP4 conjugative machinery carried by the donor strain. In the present study, we demonstrate that bacteriophage Mu, which was deliberately introduced during the original construction of the widely used donor strains SM10 λpir and S17-1 λpir, is silently transferred to Escherichia coli recipient cells at high frequency, bot

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10

Zhilina, Tatyana N., Daria G. Zavarzina, Ekaterina N. Detkova, Ekaterina O. Patutina, and Boris B. Kuznetsov. "Fuchsiella ferrireducens sp. nov., a novel haloalkaliphilic, lithoautotrophic homoacetogen capable of iron reduction, and emendation of the description of the genus Fuchsiella." International Journal of Systematic and Evolutionary Microbiology 65, Pt_8 (August 1, 2015): 2432–40. http://dx.doi.org/10.1099/ijs.0.000278.

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Two strains of haloalkaliphilic homoacetogenic bacteria capable of iron reduction, Z-7101T and Z-7102, were isolated from soda lake Tanatar III (Altai, Russia). Cells of both strains were flexible, motile, Gram-negative, spore-forming rods. The strains were mesophilic and obligately alkaliphilic: the pH range for growth was 8.5–10.2 (pHopt 9.8). Growth depended on carbonate and chloride ions. The strains were able to grow chemolithoautotrophically on H2+CO2, producing acetate as the only metabolic product. In medium with carbonates as the only potential electron acceptor, the following substra

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11

Pappas, Katherine M., and Stephen C. Winans. "Plant Transformation by Coinoculation with a Disarmed Agrobacterium tumefaciens Strain and an Escherichia coli Strain Carrying Mobilizable Transgenes." Applied and Environmental Microbiology 69, no. 11 (November 2003): 6731–39. http://dx.doi.org/10.1128/aem.69.11.6731-6739.2003.

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ABSTRACT Transformation of Nicotiana tabacum leaf explants was attempted with Escherichia coli as a DNA donor either alone or in combination with Agrobacterium tumefaciens. We constructed E. coli donor strains harboring either the promiscuous IncP-type or IncN-type conjugal transfer system and second plasmids containing the respective origins of transfer and plant-selectable markers. Neither of these conjugation systems was able to stably transform plant cells at detectable levels, even when VirE2 was expressed in the donor cells. However, when an E. coli strain expressing the IncN-type conjug

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12

Hardiman, C. A., R. A. Weingarten, S. Conlan, P. Khil, J. P. Dekker, A. J. Mathers, A. E. Sheppard, J. A. Segre, and K. M. Frank. "Horizontal Transfer of Carbapenemase-Encoding Plasmids and Comparison with Hospital Epidemiology Data." Antimicrobial Agents and Chemotherapy 60, no. 8 (June 6, 2016): 4910–19. http://dx.doi.org/10.1128/aac.00014-16.

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ABSTRACTCarbapenemase-producing organisms have spread worldwide, and infections with these bacteria cause significant morbidity. Horizontal transfer of plasmids carrying genes that encode carbapenemases plays an important role in the spread of multidrug-resistant Gram-negative bacteria. Here we investigate parameters regulating conjugation using anEscherichia colilaboratory strain that lacks plasmids or restriction enzyme modification systems as a recipient and also using patient isolates as donors and recipients. Because conjugation is tightly regulated, we performed a systematic analysis of

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13

Kos, Ivan, Milena Jadrijević-Mladar, Ivan Butula, Mladen Biruš, Gordana Maravić-Vlahoviček, and Sanja Dabelić. "Synthesis, antibacterial and cytotoxic activity evaluation of hydroxyurea derivatives." Acta Pharmaceutica 63, no. 2 (June 1, 2013): 175–91. http://dx.doi.org/10.2478/acph-2013-0014.

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5 Synthesis and biological evaluation of a series (N = 16) of cyclic and acyclic hydroxyurea derivatives, including benzotriazole-, isocyanuric acid- and biuret-containing compounds, are disclosed. 1-N-(benzyloxycarbamoyl)benzotriazole was used as a benzyloxyisocyanate donor, a useful intermediate in the preparation of substituted hydroxyurea. Antibacterial activities of synthesized hydroxyurea derivatives were tested on three E. coli strains, i.e., a strain susceptible to antibiotics, a strain resistant to macrolide antibiotics and a strain resistant to aminoglycoside antibiotics. Six compoun

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14

Van Zutphen, L. F. M., M. Den Bieman, A. Lankhorst, and P. Demant. "Segregation of genes from donor strain during the production of recombinant congenic strains." Laboratory Animals 25, no. 3 (July 1991): 193–97. http://dx.doi.org/10.1258/002367791780808329.

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15

Licht, Tine Rask, Dorthe Laugesen, Lars Bogø Jensen, and Bodil Lund Jacobsen. "Transfer of the Pheromone-Inducible Plasmid pCF10 among Enterococcus faecalis Microorganisms Colonizing the Intestine of Mini-Pigs." Applied and Environmental Microbiology 68, no. 1 (January 2002): 187–93. http://dx.doi.org/10.1128/aem.68.1.187-193.2002.

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ABSTRACT A new animal model, the streptomycin-treated mini-pig, was developed in order to allow colonization of defined strains of Enterococcus faecalis in numbers sufficient to study plasmid transfer. Transfer of the pheromone-inducible pCF10 plasmid between streptomycin-resistant strains of E. faecalis OG1 was investigated in the model. The plasmid encodes resistance to tetracycline. Numbers of recipient, donor, and transconjugant bacteria were monitored by selective plating of fecal samples, and transconjugants were subsequently verified by PCR. After being ingested by the mini-pigs, the re

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16

Ike, Yasuyoshi, Koichi Tanimoto, Haruyoshi Tomita, Kunio Takeuchi, and Shuhei Fujimoto. "Efficient Transfer of the Pheromone-IndependentEnterococcus faecium Plasmid pMG1 (Gmr) (65.1 Kilobases) to Enterococcus Strains during Broth Mating." Journal of Bacteriology 180, no. 18 (September 15, 1998): 4886–92. http://dx.doi.org/10.1128/jb.180.18.4886-4892.1998.

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ABSTRACT Plasmid pMG1 (65.1 kb) was isolated from a gentamicin-resistantEnterococcus faecium clinical isolate and was found to encode gentamicin resistance. EcoRI restriction of pMG1 produced five fragments, A through E, with molecular sizes of 50.2, 11.5, 2.0, 0.7, and 0.7 kb, respectively. The clockwise order of the fragments was ACDEB. pMG1 transferred at high frequency toEnterococcus strains in broth mating. pMG1 transferred between Enterococcus faecalis strains, betweenE. faecium strains, and between E. faecium andE. faecalis strains at a frequency of approximately 10−4 per donor cell aft

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17

Zhao, Shuqing, Margaret Duncan, Joshua Tomberg, Christopher Davies, Magnus Unemo, and Robert A. Nicholas. "Genetics of Chromosomally Mediated Intermediate Resistance to Ceftriaxone and Cefixime in Neisseria gonorrhoeae." Antimicrobial Agents and Chemotherapy 53, no. 9 (June 15, 2009): 3744–51. http://dx.doi.org/10.1128/aac.00304-09.

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ABSTRACT All strains of Neisseria gonorrhoeae with reduced susceptibility to ceftriaxone and cefixime (cephalosporin-intermediate-resistant [Cephi] strains) contain a mosaic penA allele encoding penicillin-binding protein 2 (PBP 2) with nearly 60 amino acid differences compared to the sequence of wild-type PBP 2, together with a set of resistance determinants (i.e., mtrR, penB, and/or ponA1) that are required for high-level penicillin resistance. To define the individual contributions of these determinants to reduced susceptibility to ceftriaxone and cefixime, we created isogenic strains conta

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18

Peng, Peng, Tobias Goris, Yue Lu, Bart Nijsse, Anna Burrichter, David Schleheck, Jasper J. Koehorst, et al. "Organohalide-respiring Desulfoluna species isolated from marine environments." ISME Journal 14, no. 3 (January 2, 2020): 815–27. http://dx.doi.org/10.1038/s41396-019-0573-y.

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AbstractThe genus Desulfoluna comprises two anaerobic sulfate-reducing strains, D. spongiiphila AA1T and D. butyratoxydans MSL71T, of which only the former was shown to perform organohalide respiration (OHR). Here we isolated a third strain, designated D. spongiiphila strain DBB, from marine intertidal sediment using 1,4-dibromobenzene and sulfate as the electron acceptors and lactate as the electron donor. Each strain harbors three reductive dehalogenase gene clusters (rdhABC) and corrinoid biosynthesis genes in their genomes, and dehalogenated brominated but not chlorinated organohalogens. T

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Moens, F., C. Duysburgh, P. van den Abbeele, M. Morera, and M. Marzorati. "Lactobacillus rhamnosus GG and Saccharomyces cerevisiae boulardii exert synergistic antipathogenic activity in vitro against enterotoxigenic Escherichia coli." Beneficial Microbes 10, no. 8 (December 9, 2019): 923–35. http://dx.doi.org/10.3920/bm2019.0064.

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Short-term colonic in vitro batch incubations were performed to elucidate the possible synergistic effects of Lactobacillus rhamnosus GG (CNCM-I-4798) and Saccharomyces cerevisiae boulardii (CNCM-I-1079) (associated in Smebiocta/Smectaflora Protect®) on the colonic microbial fermentation process, as well as their antipathogenic activity against enterotoxigenic Escherichia coli (LMG2092) (ETEC). These incubations adequately simulate the native microbiota and environmental conditions of the proximal colon of both adult and toddler donors, including the colonic mucosal layer. Results indicated th

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Nguyen, Kiet T., Kristina Piastro, Todd A. Gray, and Keith M. Derbyshire. "Mycobacterial Biofilms Facilitate Horizontal DNA Transfer between Strains of Mycobacterium smegmatis." Journal of Bacteriology 192, no. 19 (July 30, 2010): 5134–42. http://dx.doi.org/10.1128/jb.00650-10.

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ABSTRACT Conjugal transfer of chromosomal DNA between strains of Mycobacterium smegmatis occurs by a novel mechanism. In a transposon mutagenesis screen, three transfer-defective insertions were mapped to the lsr2 gene of the donor strain mc2155. Because lsr2 encodes a nonspecific DNA-binding protein, mutations of lsr2 give rise to a variety of phenotypes, including an inability to form biofilms. In this study, we show that efficient DNA transfer between strains of M. smegmatis occurs in a mixed biofilm and that the process requires expression of lsr2 in the donor but not in the recipient stra

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Warden, Craig H., and Jerome I. Rotter. "Genetics of Complex Disease: From Mouse to Man and Back." Canadian Journal of Gastroenterology 9, no. 3 (1995): 169–74. http://dx.doi.org/10.1155/1995/842672.

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Identification of genes underlying complex traits has been difficult, but combined application of novel methods and mouse models provides new hope. Rare monogenic syndromes, and candidate gene and biochemical approaches are sometimes useful, but each of these approaches also has limitations. Some problems that prevent identification and isolation of genes underlying complex disease can be avoided by the use of whole genome mapping of mouse crosses or of human families. Mice have many advantages for the study of complex disease, including an extensive genetic map. A generic method has recently

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22

Seyama, Shoji, Takeaki Wajima, Hidemasa Nakaminami та Norihisa Noguchi. "Clarithromycin Resistance Mechanisms of Epidemic β-Lactamase-Nonproducing Ampicillin-Resistant Haemophilus influenzae Strains in Japan". Antimicrobial Agents and Chemotherapy 60, № 5 (7 березня 2016): 3207–10. http://dx.doi.org/10.1128/aac.00163-16.

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ABSTRACTThe aim of this study was to clarify the clarithromycin resistance mechanisms of β-lactamase-nonproducing ampicillin-resistantHaemophilus influenzaestrains. In all clarithromycin-resistant strains, the transcript level ofacrBwas significantly elevated, and these strains had a frameshift mutation inacrR. Introduction of theacrRmutation intoH. influenzaeRd generated a clarithromycin-resistant transformant with the same MIC as the donor strain. Our results indicate that theacrRmutation confers clarithromycin resistance by the increasing the transcription ofacrB.

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23

Safari, Zahra, Aurélia Bruneau, Magali Monnoye, Mahendra Mariadassou, Catherine Philippe, Kurt Zatloukal, and Philippe Gérard. "Murine Genetic Background Overcomes Gut Microbiota Changes to Explain Metabolic Response to High-Fat Diet." Nutrients 12, no. 2 (January 21, 2020): 287. http://dx.doi.org/10.3390/nu12020287.

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Interactions of diet, gut microbiota, and host genetics play essential roles in the development of metabolic diseases. A/J and C57BL/6J (C57) are two mouse strains known to display different susceptibilities to metabolic disorders. In this context, we analyzed gut microbiota composition in A/J and C57 mice, and assessed its responses to high-fat diet (HFD) and antibiotic (AB) treatment. We also exchanged the gut microbiota between the two strains following AB treatment to evaluate its impact on the metabolism. We showed that A/J and C57 mice have different microbiome structure and composition

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Bi, L., R. Sarkar, T. Naas, AM Lawler, J. Pain, SL Shumaker, V. Bedian, and HH Jr Kazazian. "Further characterization of factor VIII-deficient mice created by gene targeting: RNA and protein studies." Blood 88, no. 9 (November 1, 1996): 3446–50. http://dx.doi.org/10.1182/blood.v88.9.3446.bloodjournal8893446.

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Previously we created two strains of factor VIII-deficient mice by insertion of a neo gene into (1) the 3′ end of exon 16 and (2) exon 17 of the factor VIII gene. Affected mice of both strains have no plasma factor VIII activity, yet are healthy with no spontaneous bleeding. Factor VIII-deficient females bred with affected males survive pregnancy and delivery. We used reverse transcriptase-polymerase chain reaction of liver RNA to characterize factor VIII mRNA processing. Factor VIII mRNA of the exon 16 knockout strain contains neo sequences plus 17 bp of intron 16 due to use of a cryptic dono

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Samuels, A. Lacey, Erich Lanka, and Julian E. Davies. "Conjugative Junctions in RP4-Mediated Mating ofEscherichia coli." Journal of Bacteriology 182, no. 10 (May 15, 2000): 2709–15. http://dx.doi.org/10.1128/jb.182.10.2709-2715.2000.

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ABSTRACT The physical association of bacteria during conjugation mediated by the IncPα plasmid RP4 was investigated. Escherichia colimating aggregates prepared on semisolid medium were ultrarapidly frozen using copper block freezing, followed by freeze substitution, thin sectioning, and transmission electron microscopy. In matings where the donor bacteria contained conjugative plasmids, distinctive junctions were observed between the outer membranes of the aggregates of mating cells. An electron-dense layer linked the stiffly parallel outer membranes in the junction zone, but there were no cyt

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Gord Noshahri, Najme, Jamshid Fooladi, Christoph Syldatk, Ulrike Engel, Majid M. Heravi, Mohammad Zare Mehrjerdi та Jens Rudat. "Screening and Comparative Characterization of Microorganisms from Iranian Soil Samples Showing ω-Transaminase Activity toward a Plethora of Substrates". Catalysts 9, № 10 (22 жовтня 2019): 874. http://dx.doi.org/10.3390/catal9100874.

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In this study, soil microorganisms from Iran were screened for ω-transaminase (ω-TA) activity based on growth on minimal media containing (rac)-α-methylbenzylamine (rac-α-MBA) as a sole nitrogen source. Then, for the selection of strains with high enzyme activity, a colorimetric o-xylylendiamine assay was conducted. The most promising strains were identified by 16S rDNA sequencing. Five microorganisms showing high ω-TA activity were subjected to determine optimal conditions for ω-TA activity, including pH, temperature, co-solvent, and the specificity of the ω-TA toward different amine donors a

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Dingman, Douglas W. "Conjugative transposition of Tn916 and Tn925 in Bacillus popilliae." Canadian Journal of Microbiology 45, no. 6 (July 15, 1999): 530–35. http://dx.doi.org/10.1139/w99-036.

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Interspecies transfer of the conjugative transposons Tn916 and Tn925 into B. popilliae Pj1 occurred using Enterococcus faecalis and Bacillus subtilis CU4049 as transposon donors. Tn916 was stably maintained in B. popilliae Pj1 following growth without selective pressure and was successfully introduced into the plasmid-containing B. popilliae strains NRRL B-2524, Ch1, and KLN4 using E. faecalis CG110. In B. popilliae, expression of the tetracycline resistant determinants on Tn916 and Tn925 provided resistance to 25 μg/mL and 50 μg/mL tetracycline, respectively. An erythromycin resistant determi

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Kim, Joo-Sung, Donna K. Carver, and Sophia Kathariou. "Natural Transformation-Mediated Transfer of Erythromycin Resistance in Campylobacter coli Strains from Turkeys and Swine." Applied and Environmental Microbiology 72, no. 2 (February 2006): 1316–21. http://dx.doi.org/10.1128/aem.72.2.1316-1321.2006.

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ABSTRACT Erythromycin resistance in Campylobacter coli from meat animals is frequently encountered and could represent a substantial barrier to antibiotic treatment of human infections. Erythromycin resistance in this organism has been associated with a point mutation (A2075G) in the 23S rRNA gene. However, the mechanisms responsible for possible dissemination of erythromycin resistance in C. coli remain poorly understood. In this study, we investigated transformation-mediated acquisition of erythromycin resistance by genotypically diverse C. coli strains from turkeys and swine, with total gen

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Hoffmann, Andrea, Torsten Thimm, Marcus Dröge, Edward R. B. Moore, Jean Charles Munch, and Christoph C. Tebbe. "Intergeneric Transfer of Conjugative and Mobilizable Plasmids Harbored by Escherichia coli in the Gut of the Soil Microarthropod Folsomia candida(Collembola)." Applied and Environmental Microbiology 64, no. 7 (July 1, 1998): 2652–59. http://dx.doi.org/10.1128/aem.64.7.2652-2659.1998.

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ABSTRACT The gut of the soil microarthropod Folsomia candidaprovides a habitat for a high density of bacterial cells (T. Thimm, A. Hoffmann, H. Borkott, J. C. Munch, and C. C. Tebbe, Appl. Environ. Microbiol. 64:2660–2669, 1998). We investigated whether these gut bacteria act as recipients for plasmids from Escherichia coli. Filter mating with E. coli donor cells and collected feces of F. candida revealed that the broad-host-range conjugative plasmid pRP4-luc (pRP4 with a luciferase marker gene) transferred to fecal bacteria at estimated frequencies of 5.4 × 10−1 transconjugants per donor. The

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Poinsot, Denis, Kostas Bourtzis, George Markakis, Charalambos Savakis, and Hervé Merçot. "Wolbachia Transfer from Drosophila melanogaster into D. simulans: Host Effect and Cytoplasmic Incompatibility Relationships." Genetics 150, no. 1 (September 1, 1998): 227–37. http://dx.doi.org/10.1093/genetics/150.1.227.

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Abstract Wolbachia are maternally transmitted endocellular bacteria causing a reproductive incompatibility called cytoplasmic incompatibility (CI) in several arthropod species, including Drosophila. CI results in embryonic mortality in incompatible crosses. The only bacterial strain known to infect Drosophila melanogaster (wDm) was transferred from a D. melanogaster isofemale line into uninfected D. simulans isofemale lines by embryo microinjections. Males from the resulting transinfected lines induce &gt;98% embryonic mortality when crossed with uninfected D. simulans females. In contrast

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De Milander, C., M. S. E. Wimmers, J. V. Van der Merwe, and F. Le R. Fourie. "Successful transcervical embryo transfer in two strains of mice." Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie 7, no. 1 (March 17, 1988): 26–27. http://dx.doi.org/10.4102/satnt.v7i1.895.

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Two strains of mice (a black Balb/C x C5 BL and a white NMRI strain) were stimulated to superovulate and allowed to copulate with males of a similar strain. Blastocysts were obtained from black donor animals after cervical dislocation and flushing of the fallopian tubes 3,5 days after copulation. A total of 460 blastocysts were flushed and 6 blastocysts were transfered to each of the 71 white surrogate individuals which had copulated 2,5 days previously. A total of 51 births were documented with a 31,4% occurrence of black phenotypic animals in the litter.

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Zverlov, Vladimir, Michael Klein, Sebastian Lücker, Michael W. Friedrich, Josef Kellermann, David A. Stahl, Alexander Loy, and Michael Wagner. "Lateral Gene Transfer of Dissimilatory (Bi)Sulfite Reductase Revisited." Journal of Bacteriology 187, no. 6 (March 15, 2005): 2203–8. http://dx.doi.org/10.1128/jb.187.6.2203-2208.2005.

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ABSTRACT In contrast to previous findings, we demonstrate that the dissimilatory (bi)sulfite reductase genes (dsrAB) of Desulfobacula toluolica were vertically inherited. Furthermore, Desulfobacterium anilini and strain mXyS1 were identified, by dsrAB sequencing of 17 reference strains, as members of the donor lineage for those gram-positive Desulfotomaculum species which laterally acquired dsrAB.

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Ben Fguira, Ines, Zaineb Fourati, Fakher Kamoun, Slim Tounsi, and Samir Jaoua. "Isolation of the Bacillus thuringiensis plasmid carrying Bacthuricin F4 coding genes and evidence of its conjugative transfer." Journal of Infection in Developing Countries 8, no. 06 (June 11, 2014): 727–32. http://dx.doi.org/10.3855/jidc.3552.

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Introduction: Conjugation is an excellent natural mode of DNA transfer in vivo between bacteria, particularly when these conjugative elements carry technological traits such as bacteriocin encoding genes. In the present work, the bacteriocinogenic plasmid pIBF4 from Bacillus thuringiensis responsible of Bacthuricin F4 synthesis was isolated and characterized. Methodology: To isolate pIBF4, the total plasmid DNA from a non-bacteriocin transposant carrying the mini-Tn10 spectinomycin selective marker was extracted and used to transform Escherichia coli strain Top10. PIBF4 was extracted from the

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Sorokin, Dimitry Yu, Tatjana P. Tourova, Gesche Braker, and Gerard Muyzer. "Thiohalomonas denitrificans gen. nov., sp. nov. and Thiohalomonas nitratireducens sp. nov., novel obligately chemolithoautotrophic, moderately halophilic, thiodenitrifying Gammaproteobacteria from hypersaline habitats." International Journal of Systematic and Evolutionary Microbiology 57, no. 7 (July 1, 2007): 1582–89. http://dx.doi.org/10.1099/ijs.0.65112-0.

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A novel group of moderately halophilic, obligately chemolithoautotrophic, sulfur-oxidizing Gammaproteobacteria was found in sediments of various inland hypersaline lakes and a solar saltern. These bacteria were enriched and isolated with thiosulfate as electron donor and nitrate as electron acceptor at 2 M NaCl. Ten isolates (HLD strains) were long non-motile rods. They grew anaerobically as complete denitrifiers, and aerobically under micro-oxic conditions. Sulfate was the final product of thiosulfate and sulfide oxidation, and nitrite and N2O were intermediates of nitrate reduction to N2. Th

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Chou, Sunwen. "Acquisition of Donor Strains of Cytomegalovirus by Renal-Transplant Recipients." New England Journal of Medicine 314, no. 22 (May 29, 1986): 1418–23. http://dx.doi.org/10.1056/nejm198605293142205.

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Sanford, Robert A., James R. Cole, and James M. Tiedje. "Characterization and Description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an Aryl-Halorespiring Facultative Anaerobic Myxobacterium." Applied and Environmental Microbiology 68, no. 2 (February 2002): 893–900. http://dx.doi.org/10.1128/aem.68.2.893-900.2002.

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ABSTRACT Five strains were isolated which form a physiologically and phylogenetically coherent group of chlororespiring microorganisms and represent the first taxon in the Myxobacteria capable of anaerobic growth. The strains were enriched and isolated from various soils and sediments based on their ability to grow using acetate as an electron donor and 2-chlorophenol (2-CPh) as an electron acceptor. They are slender gram-negative rods with a bright red pigmentation that exhibit gliding motility and form spore-like structures. These unique chlororespiring myxobacteria also grow with 2,6-dichlo

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Pannekoek, Yvonne, Steven M. Westenberg, Jan de Vries, Sjoerd Repping, Lodewijk Spanjaard, Paul P. Eijk, Arie van der Ende, and Jacob Dankert. "PCR Assessment of Chlamydia trachomatisInfection of Semen Specimens Processed for Artificial Insemination." Journal of Clinical Microbiology 38, no. 10 (2000): 3763–67. http://dx.doi.org/10.1128/jcm.38.10.3763-3767.2000.

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In order to ascertain the microbiological quality of stored semen specimens processed for artificial insemination by a donor (AID), we developed a PCR assay targeting the chlamydial plasmid to detectChlamydia trachomatis in semen. The lower limit of detection of this assay corresponded to 2.5 to 5 elementary bodies per μl of semen. A total of 669 cryopreserved ejaculates from 97 asymptomatic donors were tested for C. trachomatisinfection. Twelve ejaculates, originating from four donors, were found to be positive, indicating a 4% prevalence of C. trachomatis infection among the donor population

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MURINDA, SHELTON E., SHU-MIN LIU, ROBERT F. ROBERTS, and RICHARD A. WILSON. "Colicinogeny among Escherichia coli Serotypes, Including O157:H7, Representing Four Closely Related Diarrheagenic Clones." Journal of Food Protection 61, no. 11 (November 1, 1998): 1431–38. http://dx.doi.org/10.4315/0362-028x-61.11.1431.

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Twenty-seven diarrheagenic Escherichia coli (DEC) strains from five closely related, genetically distinct clones (DEC 3, 4, 8, 9, and 10), representing serotypes commonly associated with Shiga-like toxin production, i.e., 015:H−, 026:(H11, H−), 0111:(H8, H11, H−), and O157:H7, were evaluated for colicinogeny on Luria agar or Luria agar containing 0.25 μg/ml mitomycin C to induce colicin production. Ten (37%) of the DEC strains tested were colicinogenic. One of 11 serotype O157:H7 strains, DEC strain 4E, produced a colicin identified as Col D. DEC strains 8B, 9D, and 10B produced Col E1, wherea

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Machielsen, Ronnie, Roland J. Siezen, Sacha A. F. T. van Hijum, and Johan E. T. van Hylckama Vlieg. "Molecular Description and Industrial Potential of Tn6098Conjugative Transfer Conferring Alpha-Galactoside Metabolism inLactococcus lactis." Applied and Environmental Microbiology 77, no. 2 (November 29, 2010): 555–63. http://dx.doi.org/10.1128/aem.02283-10.

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ABSTRACTA novel 51-kb conjugative transposon ofLactococcus lactis, designated Tn6098, encoding the capacity to utilize α-galactosides such as raffinose and stachyose, was identified and characterized. Alpha-galactosides are a dominant carbon source in many plant-derived foods. Most dairy lactococcus strains are unable to use α-galactosides as a growth substrate, yet many of these strains are known to have beneficial industrial traits. Conjugal transfer of Tn6098was demonstrated from the plant-derived donor strainL. lactisKF147 to the recipientL. lactisNZ4501, a derivative of the dairy model st

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Jin, Hong, Helen Zhou, Bin Lu, and George Kemble. "Imparting Temperature Sensitivity and Attenuation in Ferrets to A/Puerto Rico/8/34 Influenza Virus by Transferring the Genetic Signature for Temperature Sensitivity from Cold-Adapted A/Ann Arbor/6/60." Journal of Virology 78, no. 2 (January 15, 2004): 995–98. http://dx.doi.org/10.1128/jvi.78.2.995-998.2004.

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ABSTRACT The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus with these four introduced ts loci exhibited both ts and att phenotypes similar to those of MDV-A, which could be used as a donor virus for manufacturing large quantities of inactivated influenza virus vaccine against potential pandemic strains.

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Klink, Magdalena, Maciej Cedzyński, Anna St Świerzko, Henryk Tchórzewski, and Zofia Sulowska. "Involvement of nitric oxide donor compounds in the bactericidal activity of human neutrophils in vitro." Journal of Medical Microbiology 52, no. 4 (April 1, 2003): 303–8. http://dx.doi.org/10.1099/jmm.0.04974-0.

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The bactericidal activity of human neutrophils against extracellular and facultatively intracellular bacteria was studied in the presence of the nitric oxide (NO) donors sodium nitroprusside (SNP) and 3-morpholinosydnonimine (SIN-1), a molsidomine metabolite. SNP and molsidomine are drugs commonly used as nitrovasodilators in coronary heart disease. It is demonstrated here that the NO donor compounds themselves did not affect the viability and survival of the bacterial strains tested. Neither SNP nor SIN-1 had any effect on the process of bacteria ingestion. In contrast, NO donors enhanced the

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Parshina, Sofiya N., Jan Sipma, Anne Meint Henstra, and Alfons J. M. Stams. "Carbon Monoxide as an Electron Donor for the Biological Reduction of Sulphate." International Journal of Microbiology 2010 (2010): 1–9. http://dx.doi.org/10.1155/2010/319527.

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Several strains of Gram-negative and Gram-positive sulphate-reducing bacteria (SRB) are able to use carbon monoxide (CO) as a carbon source and electron donor for biological sulphate reduction. These strains exhibit variable resistance to CO toxicity. The most resistant SRB can grow and use CO as an electron donor at concentrations up to 100%, whereas others are already severely inhibited at CO concentrations as low as 1-2%. Here, the utilization, inhibition characteristics, and enzymology of CO metabolism as well as the current state of genomics of CO-oxidizing SRB are reviewed. Carboxydotrop

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Thon, G., and A. J. Klar. "Directionality of fission yeast mating-type interconversion is controlled by the location of the donor loci." Genetics 134, no. 4 (August 1, 1993): 1045–54. http://dx.doi.org/10.1093/genetics/134.4.1045.

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Abstract Cells of homothallic strains of Schizosaccharomyces pombe efficiently switch between two mating types called P and M. The phenotypic switches are due to conversion of the expressed mating-type locus (mat1) by two closely linked silent loci, mat2-P and mat3-M, that contain unexpressed information for the P and M mating types, respectively. In this process, switching-competent cells switch to the opposite mating type in 72-90% of the cell divisions. Hence, mat2-P is a preferred donor of information to mat1 in M cells, whereas mat3-M is a preferred donor in P cells. We investigated the r

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Lesic, Biliana, and Elisabeth Carniel. "Horizontal Transfer of the High-Pathogenicity Island of Yersinia pseudotuberculosis." Journal of Bacteriology 187, no. 10 (May 15, 2005): 3352–58. http://dx.doi.org/10.1128/jb.187.10.3352-3358.2005.

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ABSTRACT The horizontal transfer of genetic elements plays a major role in bacterial evolution. The high-pathogenicity island (HPI), which codes for an iron uptake system, is present and highly conserved in various Enterobacteriaceae, suggesting its recent acquisition by lateral gene transfer. The aim of this work was to determine whether the HPI has kept its ability to be transmitted horizontally. We demonstrate here that the HPI is indeed transferable from a donor to a recipient Yersinia pseudotuberculosis strain. This transfer was observable only when the donor and recipient bacteria were c

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Milkman, Roger, Elisabeth A. Raleigh, Melissa McKane, Diane Cryderman, Patricia Bilodeau, and Kerri McWeeny. "Molecular Evolution of the Escherichia coli Chromosome. V. Recombination Patterns Among Strains of Diverse Origin." Genetics 153, no. 2 (October 1, 1999): 539–54. http://dx.doi.org/10.1093/genetics/153.2.539.

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Abstract Incorporation patterns of donor DNA into recipient chromosomes following transduction or conjugation have been studied in the progeny of a variety of Escherichia coli crosses in which donor and recipient nucleotide sequences differ by 1-3%. Series of contiguous or variously spaced PCR fragments have been amplified from each recombinant chromosome and digested with a commercial restriction endonuclease previously shown to distinguish the respective parents in a given fragment. We conclude that entering donor DNA fragments are frequently abridged (cut and shortened) before incorporation

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López-López, María José, Begonya Vicedo, Natividad Orellana, Jaime Piquer, and María M. López. "Behavior of a Virulent Strain Derived from Agrobacterium radiobacter Strain K84 After Spontaneous Ti Plasmid Acquisition." Phytopathology® 89, no. 4 (April 1999): 286–92. http://dx.doi.org/10.1094/phyto.1999.89.4.286.

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The behavior of the virulent transconjugant K84N6 derived from Agrobacterium radiobacter strain K84 after spontaneous Ti plasmid transfer in crown gall tissue in a biocontrol experiment was studied and compared with the behavior of the wild-type A. tumefaciens donor of the Ti plasmid. The main difference between the strains was a greatly reduced ability of the transconjugant to catabolize nopaline. Host range, ability to induce tumors in several fruit trees, and stability of the pathogenic determinants in isolates from tumors did not differ between the strains. Nevertheless, in a biocontrol ex

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Sawada, Akihisa, Deborah Croom-Carter, Osamu Kondo, Masahiro Yasui, Maho Koyama-Sato, Masami Inoue, Keisei Kawa, Alan B. Rickinson, and Rosemary J. Tierney. "Epstein–Barr virus latent gene sequences as geographical markers of viral origin: unique EBNA3 gene signatures identify Japanese viruses as distinct members of the Asian virus family." Journal of General Virology 92, no. 5 (May 1, 2011): 1032–43. http://dx.doi.org/10.1099/vir.0.030023-0.

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Polymorphisms in Epstein–Barr virus (EBV) latent genes can identify virus strains from different human populations and individual strains within a population. An Asian EBV signature has been defined almost exclusively from Chinese viruses, with little information from other Asian countries. Here we sequenced polymorphic regions of the EBNA1, 2, 3A, 3B, 3C and LMP1 genes of 31 Japanese strains from control donors and EBV-associated T/NK-cell lymphoproliferative disease (T/NK-LPD) patients. Though identical to Chinese strains in their dominant EBNA1 and LMP1 alleles, Japanese viruses were subtly

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48

ZHANG, GUODONG, LI MA, and MICHAEL P. DOYLE. "Potential Competitive Exclusion Bacteria from Poultry Inhibitory to Campylobacter jejuni and Salmonella." Journal of Food Protection 70, no. 4 (April 1, 2007): 867–73. http://dx.doi.org/10.4315/0362-028x-70.4.867.

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The objective of this study was to isolate from chickens potential competitive exclusion bacteria (CE) that are inhibitory to Campylobacter jejuni or Salmonella, or to both, for subsequent development of a defined CE product for use in poultry. Adult chickens from family farms, commercial farms, and broiler chicken research centers were sampled to identify and select C. jejuni–free donor chickens. A challenge treatment, which included administering perorally 106 CFU C. jejuni per chicken and determining undetectable cecal shedding of campylobacters at 4 weeks, was important for identifying the

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Pimentel, Muriel M. L., Fernanda A. Santos, Ana C. G. Teixeira, Roberta G. Izzo, Mikael A. Lima, Michelly F. Macedo, and Marcelo B. Bezerra. "Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts." Pesquisa Veterinária Brasileira 37, no. 5 (May 2017): 425–31. http://dx.doi.org/10.1590/s0100-736x2017000500001.

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ABSTRACT: This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical t

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Negrin, Cervantes D., Delyth Graham, James S. Clark, Martin W. McBride, Fiona J. Carr, Niall H. Anderson, and Anna F. Dominiczak. "Chromosome 2 Reciprocal Congenics to Evaluate the Effect of the Genetic Background on Blood Pressure." Hypertension 36, suppl_1 (October 2000): 687. http://dx.doi.org/10.1161/hyp.36.suppl_1.687-c.

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54 Background Localisation of QTL using a genome wide scan strategy is the first step towards gene identification. This is followed by the construction of congenics by which the existence of the QTL can be verified. Congenic strains for chromosome 2 using the Dahl S as the recipient and WKY and MNS as donors, showed variable blood pressure (BP) depending on the donor alleles 1 . Methods We used a speed congenic approach to produce several congenic strains of chromosome 2 using the SHRSP Gla and the WKY Gla as recipients to test the effect of the genetic background of a given congenic on BP. In

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