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Journal articles on the topic 'DOPG'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Sarkar, Malay Kumar, Mohammad Abu Sayem Karal, Marzuk Ahmed, Md Kabir Ahamed, Shareef Ahammed, Sabrina Sharmin, and Sayed Ul Alam Shibly. "Effects of osmotic pressure on the irreversible electroporation in giant lipid vesicles." PLOS ONE 16, no. 5 (May 14, 2021): e0251690. http://dx.doi.org/10.1371/journal.pone.0251690.

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Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique under different osmotic pressures (Π), and estimated the membrane tension due to Π. Two categories of GUVs were used in this study. One was prepared with a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol (chol) for obtaining more biological relevance while other with a mixture of DOPG and DOPC, with specific molar ratios. We determined the rate constant (kp) of rupture of DOPG/DOPC/chol (46/39/15)-GUVs and DOPG/DOPC (40/60)-GUVs induced by constant electric tension (σc) under different Π. The σc dependent kp values were fitted with a theoretical equation, and the corresponding membrane tension (σoseq) at swelling equilibrium under Π was estimated. The estimated membrane tension agreed well with the theoretical calculation within the experimental error. Interestingly, the values of σoseq were almost same for both types of synthesized GUVs under same osmotic pressure. We also examined the sucrose leakage, due to large osmotic pressure-induced pore formation, from the inside of DOPG/DOPC/chol(46/39/15)-GUVs. The estimated membrane tension due to large Π at which sucrose leaked out was very similar to the electric tension at which GUVs were ruptured without Π. We explained the σc and Π induced pore formation in the lipid membranes of GUVs.

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2

Crosio, Matías A., Matías A. Via, Candelaria I. Cámara, Agustin Mangiarotti, Mario G. Del Pópolo, and Natalia Wilke. "Interaction of a Polyarginine Peptide with Membranes of Different Mechanical Properties." Biomolecules 9, no. 10 (October 18, 2019): 625. http://dx.doi.org/10.3390/biom9100625.

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The membrane translocation efficiency of cell penetrating peptides (CPPs) has been largely studied, and poly-arginines have been highlighted as particularly active CPPs, especially upon negatively charged membranes. Here we inquire about the influence of membrane mechanical properties in poly-arginine adsorption, penetration and translocation, as well as the subsequent effect on the host membrane. For this, we selected anionic membranes exhibiting different rigidity and fluidity, and exposed them to the nona-arginine KR9C. Three different membrane compositions were investigated, all of them having 50% of the anionic lipid 1,2-dioleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (DOPG), thus, ensuring a high affinity of the peptide for membrane surfaces. The remaining 50% was a saturated PC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC), an unsaturated PC (1,2-dioleoyl-sn-glycero-3-phosphocholine, DOPC) or a mixture of DOPC with cholesterol. Peptide-membrane interactions were studied using four complementary models for membranes: Langmuir monolayers, Large Unilamellar Vesicles, Black Lipid Membranes and Giant Unilamellar Vesicles. The patterns of interaction of KR9C varied within the different membrane compositions. The peptide strongly adsorbed on membranes with cholesterol, but did not incorporate or translocate them. KR9C stabilized phase segregation in DPPC/DOPG films and promoted vesicle rupture. DOPC/DOPG appeared like the better host for peptide translocation: KR9C adsorbed, inserted and translocated these membranes without breaking them, despite softening was observed.

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3

Zhang, Xiaofeng, Yingyi Luo, Gang Wei, Yunrong Li, Yuechun Huang, Jiahui Huang, Chenxing Liu, et al. "Physicochemical and Antioxidant Properties of the Degradations of Polysaccharides from Dendrobium officinale and Their Suitable Molecular Weight Range on Inducing HeLa Cell Apoptosis." Evidence-Based Complementary and Alternative Medicine 2019 (August 28, 2019): 1–11. http://dx.doi.org/10.1155/2019/4127360.

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Different molecular weight polysaccharides of Dendrobium officinale (DOPs) have gradually attracted attention because of their broad biological activities. They, however, remain poorly defined whether their antitumor activity is associated with molecular weight. In this study, the physicochemical, antioxidant, and antitumor properties of DOPs, including the crude polysaccharide (DOP) and its six degradation fractions (DOP1–DOP6) extracted from Dendrobium officinale, were determined. Consequently, DOPs were mainly composed of different ratios of mannose and glucose as follows: 5.15 : 1, 4.62 : 1, 4.19 : 1, 4.46 : 1, 4.32 : 1, 4.29 : 1, and 4.23 : 1, and their molecular weights were significantly different ranging from 652.29 kDa to 11.10 kDa. With the concentration increase of DOPs, the scavenging capacity against OH and DPPH free radicals increased. The antitumor ability of DOPs was different that DOP1–DOP5 (Mw: 176.29 kDa–28.48 kDa) exhibited the best antiproliferation activity than DOP (Mw: 652.29 kDa) and DOP6 (Mw: 11.10 kDa) in HeLa cells rather than PC9, A549, and HepG2 cells. Moreover, it is worth mentioning that DOP1 and DOP5 showed stronger capability on inducing apoptosis of HeLa cells than DOP and DOP6 via the mitochondrial pathway by upregulating the ratio of the Bax/Bal-2 mRNA expression. The results demonstrated that DOPs can be used as the potential natural antioxidant and antitumor products in pharmaceutical industries, and the molecular weight is a crucial influential factor of their antitumor activity that 28.48 kDa–176.29 kDa is a suitable range we may refer to.

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4

Balali-Mood, K., T. A. Harroun, and J. P. Bradshaw. "Molecular dynamics simulations of a mixed DOPC/DOPG bilayer." European Physical Journal E 12, S1 (November 2003): 135–40. http://dx.doi.org/10.1140/epjed/e2003-01-031-3.

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5

Cawthern, Kevin, Ralph Bottenus, Dharini Rawal, and Mark Triscott. "PT and INR Interference by the Antibiotic Daptomycin in Commercial Thromboplastin Reagents: Dependence on Phospholipid Composition." Blood 108, no. 11 (November 16, 2006): 4129. http://dx.doi.org/10.1182/blood.v108.11.4129.4129.

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Abstract The antibiotic, daptomycin (brand name Cubicin, Cubist Pharmaceuticals) is approved in the US for treatment of complicated skin and skin structure infections caused by gram-positive pathogens (e.g., MRSA, methicillin-resistant Staphylococcus aureus). Recently, this antibiotic has been shown to interfere with prothrombin time (PT) assays, resulting in a prolongation of clotting times and artificial increases in the INR (International Normalized Ratio). The level of interference varies with Daptomycin concentration in the plasma, the brand of prothrombin time reagent as well as the type (normal or abnormal) of patient sample being analyzed. The bactericidal action of the antibiotic is known and is through a depolarization of bacterial membranes in the presence of physiologic calcium (Silverman, et al, Antimicrobial Agents and Chemotherapy, Aug 2003); therefore, daptomycin interference with the PT clotting assay is likely mediated through the phospholipid component of the thromboplastin formulations. To examine the dependence of daptomycin interference for a number of commercial PT formulations, testing was performed with daptomycin spiked samples of normal pooled plasma as well as abnormal samples from oral anticoagulant therapy (OAT) patients. For spiked normal samples, a small degree of prolongation in clotting times was observed with most PT reagents, with one reagent showing a significant prolongation. In OAT samples spiked with Daptomycin, the PT prolongation was greater for all formulations, with the one reagent again showing greater prolongation as for the normals. Ultimately, this variation in response was attributed to differences in phospholipid composition among the reagents, supported by previous studies of daptomycin with neutral and acidic phospholipids in the presence of calcium (D. Jung, et al., Chemistry and Biology, Jul 2004). A four-dimensional experimental design matrix was constructed using a mixture of four synthetic phospholipids (DOPC, DOPS, DOPE and DOPG), and the relationship of daptomycin-PT interference to percent composition of the phospholipids was examined. In both normal and OAT plasmas spiked with daptomycin, the major contributor to PT prolongation was the concentration of DOPG (phosphatidylglycerol) in the lipid mixture, with additional contributions correlated with DOPC (phosphatidylcholine). Because of the ubiquitous use of these phospholipids in commercial PT formulations, this work suggests that daptomycin interference should be assessed for each combination of PT reagent and INR range used.

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6

Kinkaid, A. R., and D. C. Wilton. "Enhanced hydrolysis of phosphatidylcholine by human group II non-pancreatic secreted phospholipase A2 as a result of interfacial activation by specific anions. Potential role of cholesterol sulphate." Biochemical Journal 308, no. 2 (June 1, 1995): 507–12. http://dx.doi.org/10.1042/bj3080507.

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The extracellular concentration of the Group II human non-pancreatic secreted phospholipase A2 (hnpsPLA2) is elevated in a variety of inflammatory disorders. This enzyme is remarkable because it demonstrates almost zero activity with egg phosphatidylcholine (PC) or synthetic dioleoyl-phosphatidylcholine (DOPC) as substrate, but expresses high activity with the anionic phospholipid dioleoyl-phosphatidylglycerol (DOPG), a feature shared with the Group II enzyme from rat liver. The presence of certain membrane-bound anions can enhance hydrolysis of PC by the mammalian secreted PLA2S. In this study the ability of various non-polar anions to stimulate DOPC hydrolysis by secreted PLA2S has been investigated. The naturally occurring membrane anion, cholesterol sulphate, was particularly effective in stimulating the hydrolysis of both DOPC and also 1-stearoyl-2-arachidonyl phosphatidylcholine by hnpsPLA2. Activation of DOPC hydrolysis was also achieved with dioleoyl-phosphatidylserine (DOPS); however, DOPS was less effective than cholesterol sulphate. In contrast, the dianion dioleoyl-phosphatidic acid, a known activator of pig pancreatic PLA2, failed to activate the human enzyme. It remains to be established whether cell plasma-membrane hydrolysis by extracellular hnpsPLA2 can be activated in vivo by the presence of suitable membrane anions such as cholesterol sulphate and thus promote an inflammatory response within the cell.

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7

Wang, Guangshun, Paul A. Keifer, and Alan Peterkofsky. "Short‒chain diacyl phosphatidylglycerols: which one to choose for the NMR structural determination of a membrane‒associated peptide fromEscherichia coli?" Spectroscopy 18, no. 2 (2004): 257–64. http://dx.doi.org/10.1155/2004/719137.

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Diacyl phosphatidylglycerols (PG) are the major anionic lipids in theEscherichia colimembrane. Short‒chain dihexanoyl phosphatidylglycerol (DHPG) was previously utilized for the structural determination, by NMR spectroscopy, of the peptide corresponding to the N-terminal membrane anchor of the glucose‒specific enzyme IIA (IIAGlc) fromE. coli. This study explores the possible use of lipid micelles of dioctanoyl phosphatidylglycerol (DOPG) and didecanoyl phosphatidylglycerol (DDPG) as alternatives to DHPG. At a peptide concentration of 1 mM, the minimum peptide/lipid molar ratios required for the formation of the lipid‒binding amphipathic helix are approximately 1 :40, 1 :5, and 1 :5 for DHPG, DOPG, and DDPG, respectively. Based on the lipid titration, the critical micelle concentration (CMC) of DHPG was estimated to be ~50 mM. The1H spectral linewidths of the peptide bound to a variety of lipid micelles decrease in the following order: DDPG > DOPG > DHPG. The helical regions of the peptide in different anionic lipids were elucidated based on chemical shift indexes (CSI). Residues Leu2‒Leu9, Leu2‒Val10, and Leu2‒Val10 were found to be helical in DHPG, DOPG, and DDPG, respectively, indicating that the lipid chain length had only a subtle effect on the amphipathic helix of the peptide. In light of the minimum peptide/lipid ratio and the spectral linewidth, and the CSI‒derived peptide structure, DOPG is proposed as a good compromise for structural studies of this membrane‒associated peptide by solution NMR spectroscopy.

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8

Lee, Ming-Tao, Wei-Chin Hung, and Huey W. Huang. "Rhombohedral trap for studying molecular oligomerization in membranes: application to daptomycin." Soft Matter 15, no. 21 (2019): 4326–33. http://dx.doi.org/10.1039/c9sm00323a.

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9

Tiboni, Mattia, Sofia Coppari, Luca Casettari, Michele Guescini, Mariastella Colomba, Daniele Fraternale, Andrea Gorassini, et al. "Prunus spinosa Extract Loaded in Biomimetic Nanoparticles Evokes In Vitro Anti-Inflammatory and Wound Healing Activities." Nanomaterials 11, no. 1 (December 25, 2020): 36. http://dx.doi.org/10.3390/nano11010036.

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Prunus spinosa fruits (PSF) contain different phenolic compounds showing antioxidant and anti-inflammatory activities. Innovative drug delivery systems such as biomimetic nanoparticles could improve the activity of PSF extract by promoting (i) the protection of payload into the lipidic bilayer, (ii) increased accumulation to the diseased tissue due to specific targeting properties, (iii) improved biocompatibility, (iv) low toxicity and increased bioavailability. Using membrane proteins extracted from human monocyte cell line THP-1 cells and a mixture of phospholipids, we formulated two types of PSF-extract-loaded biomimetic vesicles differing from each other for the presence of either 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) or 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG). The biological activity of free extract (PSF), compared to both types of extract-loaded vesicles (PSF-DOPCs and PSF-DOPGs) and empty vesicles (DOPCs and DOPGs), was evaluated in vitro on HUVEC cells. PSF-DOPCs showed preferential incorporation of the extract. When enriched into the nanovesicles, the extract showed a significantly increased anti-inflammatory activity, and a pronounced wound-healing effect (with PSF-DOPCs more efficient than PSF-DOPGs) compared to free PSF. This innovative drug delivery system, combining nutraceutical active ingredients into a biomimetic formulation, represents a possible adjuvant therapy for the treatment of wound healing. This nanoplatform could be useful for the encapsulation/enrichment of other nutraceutical products with short stability and low bioavailability.

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10

Shenkarev, Z. O., E. N. Lyukmanova, A. S. Paramonov, P. V. Panteleev, S. V. Balandin, M. A. Shulepko, K. S. Mineev, T. V. Ovchinnikova, M. P. Kirpichnikov, and A. S. Arseniev. "Lipid-Protein Nanodiscs Offer New Perspectives for Structural and Functional Studies of Water-Soluble Membrane-Active Peptides." Acta Naturae 6, no. 2 (June 15, 2014): 84–94. http://dx.doi.org/10.32607/20758251-2014-6-2-84-94.

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Lipid-protein nanodiscs (LPNs) are nanoscaled fragments of a lipid bilayer stabilized in solution by the apolipoprotein or a special membrane scaffold protein (MSP). In this work, the applicability of LPN-based membrane mimetics in the investigation of water-soluble membrane-active peptides was studied. It was shown that a pore-forming antimicrobial peptide arenicin-2 from marine lugworm (charge of +6) disintegrates LPNs containing both zwitterionic phosphatidylcholine (PC) and anionic phosphatidylglycerol (PG) lipids. In contrast, the spider toxin VSTx1 (charge of +3), a modifier of Kv channel gating, effectively binds to the LPNs containing anionic lipids (POPC/DOPG, 3 : 1) and does not cause their disruption. VSTx1 has a lower affinity to LPNs containing zwitterionic lipids (POPC), and it weakly interacts with the protein component of nanodiscs, MSP (charge of -6). The neurotoxin II (NTII, charge of +4) from cobra venom, an inhibitor of the nicotinic acetylcholine receptor, shows a comparatively low affinity to LPNs containing anionic lipids (POPC/DOPG, 3 : 1 or POPC/DOPS, 4 : 1), and it does not bind to LPNs/POPC. The obtained data show that NTII interacts with the LPN/POPC/DOPS surface in several orientations, and that the exchange process among complexes with different topologies proceeds fast on the NMR timescale. Only one of the possible NTII orientations allows for the previously proposed specific interaction between the toxin and the polar head group of phosphatidylserine from the receptor environment (Lesovoy et al., Biophys. J. 2009. V. 97. № 7. P. 2089-2097). These results indicate that LPNs can be used in structural and functional studies of water-soluble membrane-active peptides (probably except pore-forming ones) and in studies of the molecular mechanisms of peptide-membrane interaction.

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11

Prokofyev, Alexander, Vitya Vardanyan, and Olaf Pongs. "Differential Influence of DOPG and DOPA Anionic Phospholipids on Single Channel Conductance of KcsA." Biophysical Journal 100, no. 3 (February 2011): 98a. http://dx.doi.org/10.1016/j.bpj.2010.12.740.

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12

Elmer-Dixon, Margaret M., and Bruce E. Bowler. "Rapid quantification of vesicle concentration for DOPG/DOPC and Cardiolipin/DOPC mixed lipid systems of variable composition." Analytical Biochemistry 553 (July 2018): 12–14. http://dx.doi.org/10.1016/j.ab.2018.05.013.

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13

Hagan, Robert M., Jane Worner-Gibbs, and David C. Wilton. "The interaction of liver fatty-acid-binding protein (FABP) with anionic phospholipid vesicles: is there extended phospholipid anchorage under these conditions?" Biochemical Journal 410, no. 1 (January 29, 2008): 123–29. http://dx.doi.org/10.1042/bj20071109.

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Liver FABP (fatty-acid-binding protein) binds a variety of non-polar anionic ligands including fatty acids, fatty acyl CoAs, lysophospholipids and bile acids. Liver FABP is also able to bind to anionic phospholipid vesicles under conditions of low ionic strength, and membrane binding results in the release of bound ligand. However, the molecular interactions involved in binding to the phospholipid interface and the mechanism of ligand release are not known. Ligand release could be due to a significant conformational change in the protein at the interface or interaction of a phospholipid molecule with the ligand-binding cavity of the protein resulting in ligand displacement. Two portal mutant proteins of liver FABP, L28W and M74W, have now been used to investigate the binding of liver FABP to anionic phospholipid vesicles, monitoring changes in fluorescence and also fluorescence quenching in the presence of brominated lipids. There is a large increase in fluorescence intensity when the L28W mutant protein binds to vesicles prepared from DOPG (dioleoyl-sn-phosphatidylglycerol), but a large decrease in fluorescence intensity when the M74W mutant binds to these vesicles. The Br4-phospholipid prepared by bromination of DOPG dramatically quenches both L28W and M74W, consistent with the close proximity of a fatty acyl chain to the tryptophan residues. The binding of liver FABP to DOPG vesicles is accompanied by only a minimal change in the CD spectrum. Overall, the results are consistent with a molecule of anionic phospholipid interacting with the central cavity of the liver FABP, possibly involving the phospholipid molecule in an extended conformation.

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Dargel, Carina, Friederike Gräbitz-Bräuer, Ramsia Geisler, Pascal Fandrich, Yvonne Hannappel, Lionel Porcar, and Thomas Hellweg. "Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM." Molecules 26, no. 16 (August 16, 2021): 4959. http://dx.doi.org/10.3390/molecules26164959.

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Phosphatidylglycerols represent a large share of the lipids in the plasmamembrane of procaryotes. Therefore, this study investigates the role of charged lipids in the plasma membrane with respect to the interaction of the antiviral saponin glycyrrhizin with such membranes. Glycyrrhizin is a natural triterpenic-based surfactant found in licorice. Vesicles made of 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1’-glycerol) (DOPG)/glycyrrhizin are characterized by small-angle scattering with neutrons and X-rays (SANS and SAXS). Small-angle scattering data are first evaluated by the model-independent modified Kratky–Porod method and afterwards fitted by a model describing the shape of small unilamellar vesicles (SUV) with an internal head-tail contrast. Complete miscibility of DOPG and glycyrrhizin was revealed even at a ratio of lipid:saponin of 1:1. Additional information about the chain-chain correlation distance of the lipid/saponin mixtures in the SUV structures is obtained from wide-angle X-ray scattering (WAXS).

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15

Kawamoto, Shuhei, Masako Takasu, Takeshi Miyakawa, Ryota Morikawa, Tatsuki Oda, Shiroh Futaki, and Hidemi Nagao. "Binding of Tat peptides on DOPC and DOPG lipid bilayer membrane studied by molecular dynamics simulations." Molecular Simulation 38, no. 5 (April 2012): 366–68. http://dx.doi.org/10.1080/08927022.2010.536546.

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16

Choe, Seungho. "Molecular dynamics studies of interactions between Arg9(nona-arginine) and a DOPC/DOPG(4:1) membrane." AIP Advances 10, no. 10 (October 1, 2020): 105103. http://dx.doi.org/10.1063/5.0015665.

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17

Spengler, Dietmar, Supandi Winoto-Morbach, Sarah Kupsch, Christina Vock, Katharina Blöchle, Susanna Frank, Nele Rintz, et al. "Novel therapeutic roles for surfactant-inositols and -phosphatidylglycerols in a neonatal piglet ARDS model: a translational study." American Journal of Physiology-Lung Cellular and Molecular Physiology 314, no. 1 (January 1, 2018): L32—L53. http://dx.doi.org/10.1152/ajplung.00128.2017.

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The biological and immune-protective properties of surfactant-derived phospholipids and phospholipid subfractions in the context of neonatal inflammatory lung disease are widely unknown. Using a porcine neonatal triple-hit acute respiratory distress syndrome (ARDS) model (repeated airway lavage, overventilation, and LPS instillation into airways), we assessed whether the supplementation of surfactant (S; poractant alfa) with inositol derivatives [inositol 1,2,6-trisphosphate (IP3) or phosphatidylinositol 3,5-bisphosphate (PIP2)] or phosphatidylglycerol subfractions [16:0/18:1-palmitoyloleoyl-phosphatidylglycerol (POPG) or 18:1/18:1-dioleoyl-phosphatidylglycerol (DOPG)] would result in improved clinical parameters and sought to characterize changes in key inflammatory pathways behind these improvements. Within 72 h of mechanical ventilation, the oxygenation index (S+IP3, S+PIP2, and S+POPG), the ventilation efficiency index (S+IP3 and S+POPG), the compliance (S+IP3 and S+POPG) and resistance (S+POPG) of the respiratory system, and the extravascular lung water index (S+IP3 and S+POPG) significantly improved compared with S treatment alone. The inositol derivatives (mainly S+IP3) exerted their actions by suppressing acid sphingomyelinase activity and dependent ceramide production, linked with the suppression of the inflammasome nucleotide-binding domain, leucine-rich repeat-containing protein-3 (NLRP3)-apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC)-caspase-1 complex, and the profibrotic response represented by the cytokines transforming growth factor-β1 and IFN-γ, matrix metalloproteinase (MMP)-1/8, and elastin. In addition, IκB kinase activity was significantly reduced. S+POPG and S+DOPG treatment inhibited polymorphonuclear leukocyte activity (MMP-8 and myeloperoxidase) and the production of interleukin-6, maintained alveolar-capillary barrier functions, and reduced alveolar epithelial cell apoptosis, all of which resulted in reduced pulmonary edema. S+DOPG also limited the profibrotic response. We conclude that highly concentrated inositol derivatives and phosphatidylglycerol subfractions in surfactant preparations mitigate key inflammatory pathways in inflammatory lung disease and that their clinical application may be of interest for future treatment of the acute exudative phase of neonatal ARDS.

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Hauge, Håvard Hildeng, Dimitris Mantzilas, Vincent G. H. Eijsink, and Jon Nissen-Meyer. "Membrane-Mimicking Entities Induce Structuring of the Two-Peptide Bacteriocins Plantaricin E/F and Plantaricin J/K." Journal of Bacteriology 181, no. 3 (February 1, 1999): 740–47. http://dx.doi.org/10.1128/jb.181.3.740-747.1999.

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ABSTRACT Lactobacillus plantarum C11 produces plantaricin E/F (PlnE/F) and plantaricin J/K (PlnJ/K), two bacteriocins whose activity depends on the complementary action of two peptides (PlnE and PlnF; PlnJ and PlnK). Three of the individual Pln peptides possess some antimicrobial activity, but the highest bacteriocin activity is obtained by combining complementary peptides in about a one-to-one ratio. Circular dichroism was used to study the structure of the Pln peptides under various conditions. All four peptides were unstructured under aqueous conditions but adopted a partly alpha-helical structure in the presence of trifluoroethanol, micelles of dodecylphosphocholine, and negatively charged dioleoylphosphoglycerol (DOPG) liposomes. Far less structure was induced by zwitterionic dioleoylglycerophosphocholine liposomes, indicating that a net negative charge on the phospholipid bilayer is important for a structure-inducing interaction between (positively charged) Pln peptides and a membrane. The structuring of complementary peptides was considerably enhanced when both (PlnE and PlnF or PlnJ and PlnK) were added simultaneously to DOPG liposomes. Such additional structuring was not observed in experiments with trifluoroethanol or dodecylphosphocholine, indicating that the apparent structure-inducing interaction between complementary Pln peptides requires the presence of a phospholipid bilayer. The amino acid sequences of the Pln peptides are such that the alpha-helical structures adopted upon interaction with the membrane and each other are amphiphilic in nature, thus enabling membrane interactions.

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Královič - Kanjaková, Nina, Lukáš Hubčík, Alexander Búcsi, Mária Klacsová, Sophie Combet, José Teixeira, Juan Carlos Martínez, and Daniela Uhríková. "Calcium mediated DNA binding in non-lamellar structures formed by DOPG/glycerol monooleate." Chemistry and Physics of Lipids 239 (September 2021): 105118. http://dx.doi.org/10.1016/j.chemphyslip.2021.105118.

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20

Esseling-Ozdoba, Agnieszka, Jan W. Vos, André A. M. van Lammeren, and Anne Mie C. Emons. "Synthetic Lipid (DOPG) Vesicles Accumulate in the Cell Plate Region But Do Not Fuse." Plant Physiology 147, no. 4 (June 26, 2008): 1699–709. http://dx.doi.org/10.1104/pp.108.119842.

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21

Lyu, Yuan, Ning Xiang, Xiao Zhu, and Ganesan Narsimhan. "Potential of mean force for insertion of antimicrobial peptide melittin into a pore in mixed DOPC/DOPG lipid bilayer by molecular dynamics simulation." Journal of Chemical Physics 146, no. 15 (April 21, 2017): 155101. http://dx.doi.org/10.1063/1.4979613.

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Bondar, Ana-Nicoleta. "Phosphatidylglyerol Lipid Binding at the Active Site of an Intramembrane Protease." Journal of Membrane Biology 253, no. 6 (November 18, 2020): 563–76. http://dx.doi.org/10.1007/s00232-020-00152-z.

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AbstractTransmembrane substrate cleavage by the small Escherichia coli rhomboid protease GlpG informs on mechanisms by which lipid interactions shape reaction coordinates of membrane-embedded enzymes. Here, I review and discuss new work on the molecular picture of protein–lipid interactions that might govern the formation of the substrate–enzyme complex in fluid lipid membranes. Negatively charged PG-type lipids are of particular interest, because they are a major component of bacterial membranes. Atomistic computer simulations indicate POPG and DOPG lipids bridge remote parts of GlpG and might pre-occupy the substrate-docking site. Inhibition of catalytic activity by PG lipids could arise from ligand-like lipid binding at the active site, which could delay or prevent substrate docking. Dynamic protein–lipid H-bond networks, water access to the active site, and fluctuations in the orientation of GlpG suggest that GlpG has lipid-coupled dynamics that could shape the energy landscape of transmembrane substrate docking. Graphic Abstract

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23

Abu-Baker, Shadi, Xiaoyang Qi, Justin Newstadt, and Gary A. Lorigan. "Structural changes in a binary mixed phospholipid bilayer of DOPG and DOPS upon saposin C interaction at acidic pH utilizing 31P and 2H solid-state NMR spectroscopy." Biochimica et Biophysica Acta (BBA) - Biomembranes 1717, no. 1 (November 2005): 58–66. http://dx.doi.org/10.1016/j.bbamem.2005.09.014.

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24

Cave, Grant, Martyn Harvey, Natasha Pianca, Ivan Robertson, Jamie Sleigh, and Zimei Wu. "Intravenous DOPG liposomes do not augment pH gradient liposome supported peritoneal dialysis in treatment of acute intravenous amitriptyline intoxication in rats." Toxicology Communications 2, no. 1 (January 2018): 113–20. http://dx.doi.org/10.1080/24734306.2018.1555116.

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DALTON, Kate A., Jeffrey D. PILOT, Sanjay MALL, J. Malcolm EAST, and Anthony G. LEE. "Anionic phospholipids decrease the rate of slippage on the Ca2+-ATPase of sarcoplasmic reticulum." Biochemical Journal 342, no. 2 (August 24, 1999): 431–38. http://dx.doi.org/10.1042/bj3420431.

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Accumulation of Ca2+ by the Ca2+-ATPase of skeletal-muscle sarcoplasmic reticulum has been measured in reconstituted, sealed vesicles as a function of lipid composition. Measurements were performed in the presence of carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) to eliminate any effects of H+ transport; in the presence of FCCP, addition of valinomycin had no effect on the level or rate of accumulation of Ca2+ showing that, in the presence of FCCP, no electrical potential built up across the membrane. Levels of accumulation were low when the phospholipid was dioleoylphosphatidylcholine (DOPC), even though DOPC supports high ATPase activity. Inclusion of 10 mol% anionic phospholipid [dioleoylphosphatidic acid (DOPA) or dioleoylphosphatidylserine (DOPS)] led to higher levels of accumulation of Ca2+, 10 mol% being the optimum concentration. Cardiolipin or phosphatidylinositol 4-phosphate were more effective than DOPA or DOPS in increasing accumulation of Ca2+. Effects of anionic phospholipids were seen in the presence of an ATP-regenerating system to remove ADP, and in the presence of phosphate within the reconstituted vesicles to precipitate calcium phosphate. Rates of passive leak of Ca2+ from the reconstituted vesicles were slow. The Ca2+-accumulation process was simulated assuming either simple passive leak of Ca2+ from the vesicles or assuming slippage on the ATPase, a process in which the phosphorylated intermediate of the ATPase releases bound Ca2+ on the cytoplasmic rather than the lumenal side of the membrane. The experimental data fitted to a slippage model, with anionic phospholipids decreasing the rate of slippage.

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Awad, Tarek, Y. Okamoto, Shah Masum, and M. Yamazaki. "3P248 Formation of Cubic Phases from Large Unilamellar Vesicles of DOPG/MO Membranes Induced by Low Concentrations of Ca^<2+>." Seibutsu Butsuri 45, supplement (2005): S265. http://dx.doi.org/10.2142/biophys.45.s265_4.

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Hou, Yi, Jianhui Li, XinXin Liu, Yundan Ruan, Shun-li Chen, Qunhui Yuan, and Wei Gan. "The effect of side group on the dynamic behavior of anthracyclines on DOPG lipid membranes revealed by second harmonic generation and fluorescence." Chemical Physics 541 (January 2021): 111036. http://dx.doi.org/10.1016/j.chemphys.2020.111036.

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28

Ohta, Tetsuya, Risato Yamada, Satoshi Fujita, Taketoshi Takahata, Kohei Shiba, Sachiko Machida, and Shin-ichi Tate. "DOPG small unilamellar vesicles function as nano-carriers targeting the clustered lectin-like oxidized LDL receptor (LOX-1) on the cell surface." Journal of Drug Delivery Science and Technology 51 (June 2019): 327–36. http://dx.doi.org/10.1016/j.jddst.2019.03.014.

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29

Grimaldi, Manuela, Michela Buonocore, Mario Scrima, Ilaria Stillitano, Gerardino D’Errico, Angelo Santoro, Giuseppina Amodio, et al. "NMR Structure of the FIV gp36 C-terminal Heptad Repeat and Membrane-Proximal External Region." International Journal of Molecular Sciences 21, no. 6 (March 16, 2020): 2037. http://dx.doi.org/10.3390/ijms21062037.

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Feline immunodeficiency virus (FIV), a lentivirus causing an immunodeficiency syndrome in cats, represents a relevant model of pre-screening therapies for human immunodeficiency virus (HIV). The envelope glycoproteins gp36 in FIV and gp41 in HIV mediate the fusion of the virus with the host cell membrane. They have a common structural framework in the C-terminal region that includes a Trp-rich membrane-proximal external region (MPER) and a C-terminal heptad repeat (CHR). MPER is essential for the correct positioning of gp36 on the lipid membrane, whereas CHR is essential for the stabilization of the low-energy six-helical bundle (6HB) that is necessary for the fusion of the virus envelope with the cell membrane. Conformational data for gp36 are missing, and several aspects of the MPER structure of different lentiviruses are still debated. In the present work, we report the structural investigation of a gp36 construct that includes the MPER and part of the CHR domain (737-786gp36 CHR–MPER). Using 2D and 3D homo and heteronuclear NMR spectra on 15N and 13C double-labelled samples, we solved the NMR structure in micelles composed of dodecyl phosphocholine (DPC) and sodium dodecyl sulfate (SDS) 90/10 M: M. The structure of 737-786gp36 CHR–MPER is characterized by a helix–turn–helix motif, with a regular α-helix and a moderately flexible 310 helix, characterizing the CHR and the MPER domains, respectively. The two helices are linked by a flexible loop regulating their orientation at a ~43° angle. We investigated the positioning of 737-786gp36 CHR–MPER on the lipid membrane using spin label-enhanced NMR and ESR spectroscopies. On a different scale, using confocal microscopy imaging, we studied the effect of 737-786gp36 CHR–MPER on 1,2-dioleoyl-sn-glycero-3-phosphocholine/1,2-dioleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (DOPC/DOPG) multilamellar vesicles (MLVs). This effect results in membrane budding and tubulation that is reminiscent of a membrane-plasticizing role that is typical of MPER domains during the event in which the virus envelope merges with the host cell membrane.

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Pinheiro, Teresa J. T., Melinda J. Duer, and Anthony Watts. "Phospholipid headgroup dynamics in DOPG-d5-cytochrome c complexes as revealed by 2H and 31P NMR: The effects of a peripheral protein on collective lipid fluctuations." Solid State Nuclear Magnetic Resonance 8, no. 1 (March 1997): 55–64. http://dx.doi.org/10.1016/s0926-2040(96)01255-6.

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31

Liang, Xiaoe, Mary Ann Quinn-Allen, and William H. Kane. "Cholesterol and Phosphatidylethanolamine Enhance the Binding of Factor Va and Factor Xa to Phospholipid Vesicles and Promote Assembly of the Prothrombinase Complex on Membranes Containing Low Concentrations of Phosphatidylserine." Blood 108, no. 11 (November 16, 2006): 1712. http://dx.doi.org/10.1182/blood.v108.11.1712.1712.

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Abstract Platelet membranes are composed of cholesterol (CH) and phospholipids including phosphatidylethanolamine (PE), sphingomeylin (SM), phosphatidylcholine (PC) and phosphatidylserine (PS). Phospholipid vesicles containing 20–25% PS have been used extensively to characterize assembly and regulation of the prothrombinase complex. However, the concentration of PS on the surface of activated platelets has been determined to be only 4–13%. Smeets et al (Thromb. Res. 81:419, 1996) demonstrated that CH and PE in the presence of 0–10% PS have stimulating effects on prothrombinase activity, while SM has an inhibitory one. To further investigate the roles of CH, PE and SM in the binding of factor Va and Xa to phospholipid vesicles and assembly of the prothrombinase complex, vesicles consisting of 1–25% DOPS plus 30% CH, 30% DOPE and/or 20% SM, were prepared for prothrombinase assays as a function of DOPS concentration. Compared to individual effect of DOPS alone or DOPS plus CH, PE or SM on prothrombinase activity, a synergistic effect was observed on vesicles containing CH, DOPE and SM, which was significant in the range of 1–15% DOPS. The PS concentration required for half-maximal rates of thrombin generation was 5.3% for vesicles containing CH, DOPE and SM, compared to 10.6, 11.2, 8.3 and 12.6% for vesicles containing DOPS alone, plus CH, plus DOPE or plus SM, respectively. This demonstrates that the requirement for PS is substantially decreased in the presence of CH, PE and SM. In order to further define the mechanisms for this effect, direct binding of factor Va to vesicles containing 30% CH, 30% PE and 20% SM was investigated using a fluorescence resonance energy transfer assay. These binding experiments demonstrated that factor Va bound to vesicles containing 1, 5, 10, and 20% DOPS with Kd values of 2.88±0.43 nM, 1.15±0.17 nM, 1.16±0.13 nM, and 1.18±0.19 nM, respectively. At low concentrations of PS (≤ 5%), CH, DOPE and SM increased the binding affinity of factor Va 2 to 6-fold compared to vesicles containing only DOPS. When prothrombinase activity was measured as a function of factor Va concentration on vesicles containing 5, 10, and 20% DOPS, K1/2Va values of 0.63±0.08nM, 0.38±0.05nM, and 0.37±0.07nM were observed which were comparable to the Kd’s determined in direct factor Va binding experiments. These results indicate that factor Va binds tightly to phospholipid vesicles mimicking the phospholipid composition of activated platelets even at very low concentrations of PS. When direct binding of factor Xa to vesicles containing 5, 10, and 20% DOPS plus CH, DOPE and SM was examined, the Kd values were 70.5±6.8 nM, 62.6±6.6 nM, and 49.6±4.0 nM, respectively. When the interaction of factor Xa with membranes was investigated using prothrombinase assays, K1/2Xa values of 0.12±0.03nM, 0.04±0.01nM, and 0.01±0.01nM were observed. In conclusion, the presence of CH and PE increases the affinity of factor Va and factor Xa binding to phospholipid vesicles containing physiologic concentrations of PS. The binding of factor Va to vesicles containing 1% PS is not sufficient for assembly and function of the prothrombinase complex, suggesting that higher concentrations of PS are needed to support the binding of factor Xa and prothrombin.

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32

Yesylevskyy, S. O., A. P. Demchenko, S. Kraszewski, and C. Ramseyer. "Cholesterol Induces Uneven Curvature of Asymmetric Lipid Bilayers." Scientific World Journal 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/965230.

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A remarkable flexibility is observed in biological membranes, which allows them to form the structures of different curvatures. We addressed the question of intrinsic ability of phospholipid membranes to form highly curved structures and the role of cholesterol in this process. The distribution of cholesterol in the highly curved asymmetric DOPC/DOPS lipid bilayer was investigated by the coarse-grained molecular dynamics simulations in the membrane patches with large aspect ratio. It is shown that cholesterol induces uneven membrane curvature promoting the formation of extended flattened regions of the membrane interleaved by sharp bends. It is shown that the affinity of cholesterol to anionic DOPS or neutral DOPC lipids is curvature dependent. The cholesterol prefers DOPS to DOPC in either planar or highly curved parts of the membrane. In contrast, in the narrow interval of moderate membrane curvatures this preference is inverted. Our data suggest that there is a complex self-consistent interplay between the membrane curvature and cholesterol distribution in the asymmetric lipid bilayers. The suggested new function of cholesterol may have a biological relevance.

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33

Ash, Caroline. "The dope on L-dopa metabolism." Science 364, no. 6445 (June 13, 2019): 1043.11–1045. http://dx.doi.org/10.1126/science.364.6445.1043-k.

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34

Ermilova, Inna, and Jan Swenson. "DOPC versus DOPE as a helper lipid for gene-therapies: molecular dynamics simulations with DLin-MC3-DMA." Physical Chemistry Chemical Physics 22, no. 48 (2020): 28256–68. http://dx.doi.org/10.1039/d0cp05111j.

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35

Ranjith, N. K., Ch V. Ramana, and Ch Sasikala. "Purification and characterization of 3,4-dihydroxyphenylalanine oxidative deaminase fromRhodobacter sphaeroidesOU5." Canadian Journal of Microbiology 54, no. 10 (October 2008): 829–34. http://dx.doi.org/10.1139/w08-071.

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An enzyme involved in the catabolism of 3,4-dihydroxyphenylalanine (DOPA) was isolated from Rhodobacter sphaeroides OU5. The enzyme catalyzes the formation of 3,4-dihydroxyphenylpyruvic acid (DOPP) and ammonia from DOPA. Formation of ammonia by DOPA oxidative deaminase was O2dependent and the enzyme isolated to its homogeneity has 100% affinity for DOPA. DOPA oxidative deaminase is functional at low concentrations of the substrate (<100 μmol·L–1) and is independent of NADH. The molecular mass of the purified enzyme is ~190 kDa and the enzyme could be a pentamer of 54, 42, 34, 25, and 23 kDa subunits as determined by SDS–PAGE.

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36

Johnsen, Line, Gunnar Fimland, Dimitris Mantzilas, and Jon Nissen-Meyer. "Structure-Function Analysis of Immunity Proteins of Pediocin-Like Bacteriocins: C-Terminal Parts of Immunity Proteins Are Involved in Specific Recognition of Cognate Bacteriocins." Applied and Environmental Microbiology 70, no. 5 (May 2004): 2647–52. http://dx.doi.org/10.1128/aem.70.5.2647-2652.2004.

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ABSTRACT The immunity proteins of pediocin-like bacteriocins show a high degree of specificity with respect to the pediocin-like bacteriocin they recognize and confer immunity to. The aim of this study was to identify regions of the immunity proteins that are involved in this specific recognition. Six different hybrid immunity proteins were constructed from three different pediocin-like bacteriocin immunity proteins that have similar sequences but confer resistance to different bacteriocins. These hybrid immunity proteins were then tested for their ability to confer immunity to various pediocin-like bacteriocins. The specificities of the hybrid immunity proteins proved to be similar to those of the immunity proteins from which the C-terminal halves were derived, thus revealing that the C-terminal half of immunity proteins for pediocin-like bacteriocins contains a domain that is involved in specific recognition of the bacteriocins they confer immunity to. Moreover, the results also revealed that the effectiveness of an immunity protein is strain dependent and that its functionality thus depends in part on interplay with strain-dependent factors. To further investigate the structure-function relationship of these immunity proteins, the enterocin A and leucocin A immunity proteins (EntA-im and LeuA-im) were purified to homogeneity and structurally analyzed under various conditions by Circular dichroism (CD) spectroscopy. The results revealed that both immunity proteins are α-helical and well structured in an aqueous environment, the denaturing temperature being 78.5°C for EntA-im and 58.0°C for LeuA-im. The CD spectra also revealed that there was no further increase in the structuring or α-helical content when the immunity proteins were exposed to dodecylphosphocholine micelles or dioleoyl-l-α-phosphatidyl-dl-glycerol (DOPG) liposomes, indicating that the immunity proteins, in contrast to the bacteriocins, do not interact extensively with membranes. They may nevertheless be loosely associated with the membrane, possibly as peripheral membrane proteins, thus enabling them to interact with their cognate bacteriocin.

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37

Rorie, D. K., L. W. Hunter, and G. M. Tyce. "Dihydroxyphenylglycol as an index of neuronal uptake in dog saphenous vein." American Journal of Physiology-Heart and Circulatory Physiology 257, no. 6 (December 1, 1989): H1945—H1951. http://dx.doi.org/10.1152/ajpheart.1989.257.6.h1945.

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Dihydroxyphenylglycol (DOPEG), the metabolite of norepinephrine (NE) that arises intraneuronally, was measured together with NE in superfusates collected before, during, and after nerve stimulation and in extracts of dog saphenous vein after superfusion and electrical stimulation (ES). Different concentrations of NE in the synaptic clefts were achieved by treating tissues with corticosterone, corticosterone and yohimbine, corticosterone and cocaine, or by omitting drugs from the superfusate. NE and DOPEG were quantitated by liquid chromatography with electrochemical detection. The time courses of NE overflow and DOPEG efflux into superfusate were followed. The amounts of DOPEG in superfusates under basal conditions were two to four times higher than the amounts of NE and progressively increased during ES except in tissues with neuronal uptake inhibited. NE overflow reached a steady state within the first 6 min of ES. Increased NE concentrations in synaptic clefts resulted in increased DOPEG production except where neuronal uptake was inhibited. The increased DOPEG production during ES appears to reflect the increased rate of neuronal uptake, which results in more NE being available for intraneuronal metabolism. No evidence was found that newly formed DOPEG was delayed in leaving the tissue. Thus the increase in DOPEG production that occurs during ES may be useful as an index of neuronal uptake of NE in dog saphenous vein.

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Cherniavskyi, Yevhen K., Christophe Ramseyer, and Semen O. Yesylevskyy. "Interaction of C60 fullerenes with asymmetric and curved lipid membranes: a molecular dynamics study." Physical Chemistry Chemical Physics 18, no. 1 (2016): 278–84. http://dx.doi.org/10.1039/c5cp05838d.

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Interaction of C60 fullerenes with asymmetric and curved DOPC/DOPS bicelles is studied by the coarse-grained MD simulations. The effects caused by asymmetric lipid composition of the membrane leaflets and the curvature of the membrane are analyzed.

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39

Yang, Yuanxi, Jinlong Li, Junyi Xu, and Jing Tang. "Generalised DOPs with Consideration of the Influence Function of Signal-in-Space Errors." Journal of Navigation 64, S1 (October 14, 2011): S3—S18. http://dx.doi.org/10.1017/s0373463311000415.

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Integrated navigation using multiple Global Navigation Satellite Systems (GNSS) is beneficial to increase the number of observable satellites, alleviate the effects of systematic errors and improve the accuracy of positioning, navigation and timing (PNT). When multiple constellations and multiple frequency measurements are employed, the functional and stochastic models as well as the estimation principle for PNT may be different. Therefore, the commonly used definition of “dilution of precision (DOP)” based on the least squares (LS) estimation and unified functional and stochastic models will be not applicable anymore. In this paper, three types of generalised DOPs are defined. The first type of generalised DOP is based on the error influence function (IF) of pseudo-ranges that reflects the geometry strength of the measurements, error magnitude and the estimation risk criteria. When the least squares estimation is used, the first type of generalised DOP is identical to the one commonly used. In order to define the first type of generalised DOP, an IF of signal–in-space (SIS) errors on the parameter estimates of PNT is derived. The second type of generalised DOP is defined based on the functional model with additional systematic parameters induced by the compatibility and interoperability problems among different GNSS systems. The third type of generalised DOP is defined based on Bayesian estimation in which the a priori information of the model parameters is taken into account. This is suitable for evaluating the precision of kinematic positioning or navigation. Different types of generalised DOPs are suitable for different PNT scenarios and an example for the calculation of these DOPs for multi-GNSS systems including GPS, GLONASS, Compass and Galileo is given. New observation equations of Compass and GLONASS that may contain additional parameters for interoperability are specifically investigated. It shows that if the interoperability of multi-GNSS is not fulfilled, the increased number of satellites will not significantly reduce the generalised DOP value. Furthermore, the outlying measurements will not change the original DOP, but will change the first type of generalised DOP which includes a robust error IF. A priori information of the model parameters will also reduce the DOP.

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40

Klacsová, Mária, Janka Karlovská, Daniela Uhríková, Sérgio S. Funari, and Pavol Balgavý. "Phase behavior of the DOPE + DOPC + alkanol system." Soft Matter 10, no. 31 (June 30, 2014): 5842. http://dx.doi.org/10.1039/c4sm00530a.

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41

Rorie, D. K., L. W. Hunter, and G. M. Tyce. "Neuropeptide Y and 3,4-dihydroxyphenylglycol effluxes from artery are oxygen sensitive." American Journal of Physiology-Heart and Circulatory Physiology 261, no. 5 (November 1, 1991): H1371—H1378. http://dx.doi.org/10.1152/ajpheart.1991.261.5.h1371.

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Neuropeptide Y-like immunoreactivity (NPY-LI), norepinephrine (NE), and 3,4-dihydroxyphenylglycol (DOPEG), the intraneuronal metabolite of NE, were measured in superfusate before, during, and after electrical stimulation (ES) of nerves and in the tissue extract of dog pulmonary artery after in vitro superfusion. Vessels were stimulated at 12, 6, or 1 Hz. NE and DOPEG were quantified by high-performance liquid chromatography with electrochemical detection, NPY-LI by radioimmunoassay. The Krebs-Ringer superfusate was maintained at a partial pressure of oxygen of either 472, 100, 50, or 20 mmHg. Mean DOPEG efflux was 0.17, 0.15, 0.13, and 0.07 pmol/min during basal conditions and 0.73, 0.39, 0.30, and 0.15 pmol/min, respectively, during 12-Hz continuous stimulation using these four oxygen pressures. Stimulation-evoked efflux of NPY-LI was 1.5, 1.1, 0.3, and 0.2 fmol/min during 12-Hz stimulation. These studies provide evidence that the production and subsequent efflux of DOPEG into superfusate under resting conditions, during ES, and following ES are oxygen sensitive. Additionally, the efflux of NPY-LI from dog pulmonary artery resulting from high frequencies of ES is oxygen sensitive.

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42

Awad, Tarek S., Shah Md Masum, R. Sano, and M. Yamazaki. "1P204 Formation of Cubic Phases from Large Unilamellar Vesicles of Monoolein/ Dioleoylphsphatidylglycerol Membranes Induced by Ca^<2+>." Seibutsu Butsuri 44, supplement (2004): S80. http://dx.doi.org/10.2142/biophys.44.s80_4.

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43

Schwinn, Kathy E. "The dope on l -DOPA formation for betalain pigments." New Phytologist 210, no. 1 (February 25, 2016): 6–9. http://dx.doi.org/10.1111/nph.13901.

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44

Cheng, Meng-Hsuan, Jun-Yi Weng, Chih-Hung Chuang, Wei-Ting Liao, Yu-Fong Lai, Jia-Yu Liu, and Yi-Ping Fang. "Prolonging the Half-Life of Histone Deacetylase Inhibitor Belinostat via 50 nm Scale Liposomal Subcutaneous Delivery System for Peripheral T-Cell Lymphoma." Cancers 12, no. 9 (September 8, 2020): 2558. http://dx.doi.org/10.3390/cancers12092558.

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Lymph node metastasis is an aggressive condition characterized by poor treatment outcomes and low overall survival. Belinostat is a novel histone deacetylase (HDAC) inhibitor approved by the Food and Drug Administration (FDA) for the treatment of relapsed peripheral T-cell lymphoma (PTCL). However, the major problem is that belinostat has a short half-life of 1.1 h. In this study, we successfully prepared 50 nm liposomal colloids, which showed a controlled release pattern and excellent pharmacokinetics. The results showed that the particle size of liposomes consisting of dioleoylphosphatidylcholine (DOPC) was larger than that of those consisting of dioleoylglycerophosphoserine (DOPS). In terms of release kinetics of belinostat, the free drug was rapidly released and showed lower area under curve (AUC) exposure for in vivo pharmacokinetics. When liposomal formulations were employed, the release pattern was fitted with Hixson–Crowell models and showed sustained release of belinostat. Moreover, HuT-78 cells were able to take up all the liposomes in a concentration-dependent manner. The safety assessment confirmed hemocompatibility, and the platelet count was increased. Furthermore, the liposomes consisting of DOPC or DOPS had different behavior patterns, and their delivery to lymphatic regions should be thoroughly investigated in the future.

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45

Wu, Weizu, Dongqing Xie, and Liqun Liu. "Heterogeneous Differential Evolution with Memory Enhanced Brownian and Quantum Individuals for Dynamic Optimization Problems." International Journal of Pattern Recognition and Artificial Intelligence 32, no. 02 (November 12, 2017): 1859003. http://dx.doi.org/10.1142/s0218001418590036.

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In order to solve the dynamic optimization problem (DOP), this paper proposes to use a heterogeneous differential evolution (HDE) algorithm framework with memory enhanced Brownian and quantum (MEBQ) individual scheme. The proposed HDE/MEBQ algorithm has the following two advantages when solving DOP. First and foremost, the HDE optimization framework can satisfy the problem requirement of different characteristics. DOP is actually a continuous process to solve different kinds of optimization problems to meet new requirements when the search environmental change occurs. Therefore, HDE/MEBQ is able to fastly respond to the environmental changes of DOP as the HDE framework uses multiple populations with heterogeneous parameters and operators to meet different search requirements in various search environments. Secondly, according to the phenomenon that most of the environmental changes may not be too drastic in real-world applications, historical information in the past may be useful for finding the optimum solution in the new environment. Thus, the MEBQ scheme used in HDE/MEBQ provides helpful historical evolutionary information from the elite ancestors for guiding individuals to evolve in a new environment strongly and to obtain faster convergence and a more precise solution. We evaluated HDE/MEBQ on several DOPs from CEC 2009 and compared with several state-of-the-art dynamic evolutionary algorithms. The results show that HDE/MEBQ performs superior in statistics and gets very competitive results in most of the test conditions, especially in complex DOPs.

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Cheng, K. H., L. Ruymgaart, L. I. Liu, P. Somerharju, and I. P. Sugar. "Intramolecular excimer kinetics of fluorescent dipyrenyl lipids: 2. DOPE/DOPC membranes." Biophysical Journal 67, no. 2 (August 1994): 914–21. http://dx.doi.org/10.1016/s0006-3495(94)80553-3.

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47

Klacsová, M., A. Bóta, and P. Balgavý. "DOPC-DOPE composition dependent Lα-HII thermotropic phase transition: SAXD study." Chemistry and Physics of Lipids 198 (June 2016): 46–50. http://dx.doi.org/10.1016/j.chemphyslip.2016.05.004.

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48

Millán Vázquez de la Torre, Maria Genoveva, Luis Amador Hidalgo, and Juan Manuel Arjona Fuentes. "La denominación de origen protegida “Los Pedroches” como ruta gastronómica del jamón ibérico: análisis del perfil del visitante y evolución futura." Cuadernos de Desarrollo Rural 13, no. 77 (June 21, 2016): 63. http://dx.doi.org/10.11144/javeriana.cdr13-77.dopp.

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Las denominaciones de origen tienen una larga historia en España y el caso del jamón no es una excepción. La denominación de origen protegida del jamón ibérico “Los Pedroches” constituye un ejemplo de simbiosis entre esta figura y la de las rutas gastronómicas. En este trabajo se ha realizado un análisis del perfil del visitante de la comarca en la que esta denominación se asienta, con el objetivo de recabar información que sirva para promover la mencionada denominación como ruta gastronómica y, de esta manera, incrementar las fuentes de ingresos y los niveles de renta y empleo de la población local, potenciando asimismo la multifuncionalidad del territorio rural de la zona.

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Fernández-Ruiz, J. J., M. Cebeira, C. Agrasal, J. A. F. Tresguerres, A. Bartke, A. I. Esquifino, and J. A. Ramos. "Possible role of dopamine and noradrenaline in the regulation of prolactin secretion from an ectopic anterior pituitary gland in female rats." Journal of Endocrinology 113, no. 1 (April 1987): 45–49. http://dx.doi.org/10.1677/joe.0.1130045.

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ABSTRACT It was recently reported that anterior pituitary tissue transplanted to an ectopic site contains measurable amounts of dopamine and noradrenaline. To examine the possibility of local catecholaminergic control of prolactin secretion from ectopic pituitaries, pituitary grafted and sham-operated female rats were submitted to several pharmacological treatments modifying catecholamine synthesis. Administration of a single dose of α-methyl-p-tyrosine (α-MPT) significantly reduced dopamine content in the graft, while noradrenaline content was not modified. Similar changes in the contents of dopamine and noradrenaline after α-MPT administration were observed in the hypothalamus and in the in-situ pituitary in both grafted and sham-operated rats. Plasma concentrations of prolactin were increased in both grafted and sham-operated rats after administration of α-MPT. A single injection of l-3,4-dihydroxyphenylalanine (l-DOPA) increased dopamine content in the ectopic pituitary gland without altering the noradrenaline content, and produced similar effects in the hypothalamus and in-situ pituitary of grafted and control rats. Plasma prolactin concentrations were decreased by l-DOPA in both pituitary grafted and control rats. Administration of dl-treo-dihydroxyphenylserine (DOPS) increased noradrenaline content in the ectopic pituitary and reduced plasma prolactin concentrations in pituitary grafted rats. In contrast, injection of DOPS to control rats increased both hypothalamic noradrenaline content and plasma prolactin concentrations. These results suggest that dopamine and noradrenaline present in the ectopic pituitary tissue have a role in mediating prolactin release from pituitary transplants. J. Endocr. (1987) 113, 45–49

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50

Murugova, T. N., and P. Balgavý. "Molecular volumes of DOPC and DOPS in mixed bilayers of multilamellar vesicles." Phys. Chem. Chem. Phys. 16, no. 34 (2014): 18211–16. http://dx.doi.org/10.1039/c4cp01980f.

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Specific volume of 1,2-dioleoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phospho-l-serine mixtures in bilayers linearly depends on the molar fraction of the constituents, indicating an ideal volume mixing.

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