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Relevant bibliographies by topics / Dot-ELISA

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Journal articles

Academic literature on the topic 'Dot-ELISA'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 March 2023

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Journal articles on the topic "Dot-ELISA"

1

Gerbig, Donald G., Christopher J. Fenk, and Amy S. Goodhart. "The Dot Blot ELISA." American Biology Teacher 62, no. 8 (2000): 583–87. http://dx.doi.org/10.1662/0002-7685(2000)062[0583:tdbe]2.0.co;2.

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Khanal, Doj Raj, Eliza Ranjit, Sanjeev Kumar Adhikari, Bimal Ghemosu, Meera Prajapati, and Swoyam Prakash Shrestha. "Seroprevalence of mycoplasmosis in poultry of Bhaktapur." International Journal of Applied Sciences and Biotechnology 6, no. 1 (2018): 23–26. http://dx.doi.org/10.3126/ijasbt.v6i1.19103.

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Mycoplasmas are amongst the avian pathogens that causes chronic respiratory and joint diseases incurring a huge economic loss to poultry industry of Nepal. Among different species of Mycoplasmas, we investigate Mycoplasma gallisepticum-synoviae from the serum samples of the poultry using Enzyme Linked Immuno Sorbent Assay (ELISA) and dot-ELISA test of ImmunoComb. These tests rely on the antibodies present in the serum samples which binds to the pre-coated antigen in the ELISA/developing plates. A total of 92 sera samples were collected, of which 62 were from broiler and 30 were from layers. Of

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3

Bosompem, K. M., R. A. Masake, R. K. G. Assoku, E. A. Opiyo, and V. M. Nantulya. "Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossinaspp.)." Parasitology 112, no. 2 (1996): 205–11. http://dx.doi.org/10.1017/s0031182000084778.

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SummaryA rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossinaspp.), was field tested alongside the standard fly dissection method on a ranch in south eastern Kenya. Of 104G. pallidipesdissected, 2 were found to be infected with trypanosomes in their midguts. By the dissection method the infecting trypanosome species could not be identified, as both flies had no salivary gland infections. However, using the dot-ELISA, the 2 flies were shown to be infected withTrypanosoma congolensein their midguts. The midguts of an add

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4

Requejo, Henry I. Z., Maria das Graças A. Alkmin, Regina G. Almeida, et al. "Dot-enzyme-linked immunosorbent assay (Dot-ELISA) for detection of pneumococcal polysaccharide antigens in pleural fluid effusion samples.: Comparison with bacterial culture, counterimmunoelectrophoresis and latex agglutination." Revista do Instituto de Medicina Tropical de São Paulo 36, no. 6 (1994): 531–37. http://dx.doi.org/10.1590/s0036-46651994000600010.

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A dot-enzyme-linked immunosorbent assay (Dot-ELISA) for pneumococcal antigen detection was standardized in view of the need for a rapid and accurate immunodiagnosis of acute pneumococcal pneumonia. A total of 442 pleural fluid effusion samples (PFES) from children with clinical and laboratory diagnoses of acute bacterial pneumonia, plus 38 control PFES from tuberculosis patients and 20 negative control serum samples from healthy children were evaluated by Dot-ELISA. The samples were previously treated with 0.1 M EDTA pH 7.5 at 90°C for 10 min and dotted on nitrocellulose membrane. Pneumococcal

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5

Macedo-Silva, A., S. F. C. Barbosa, M. G. A. Alkmin, A. J. Vaz, M. Shimokomaki, and A. Tenuta-Filho. "Hamburger meat identification by dot-ELISA." Meat Science 56, no. 2 (2000): 189–92. http://dx.doi.org/10.1016/s0309-1740(00)00039-5.

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6

Prakash, D., P. B. Parab, and R. N. Sharma. "Immunodiagnosis of dracunculiasis by dot-ELISA." Annals of Tropical Medicine & Parasitology 87, no. 2 (1993): 195–99. http://dx.doi.org/10.1080/00034983.1993.11812754.

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7

Bokoliya, Suresh, Shripad Patil, Madhu Nagappa, and Arun Taly. "A Simple, Rapid and Non-Radiolabeled Immune Assay to Detect Anti-AChR Antibodies in Myasthenia Gravis." Laboratory Medicine 50, no. 3 (2018): 229–35. http://dx.doi.org/10.1093/labmed/lmy038.

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AbstractObjectiveTo assess the practicality of dot-blot testing for rapid and sensitive detection of the antiacetylcholine receptor (anti-AChR) antibodies in myasthenia gravis (MG).MethodsIn this case-control study, we tested serum specimens of 85 patients with MG, 85 healthy control individuals, and 85 patients without MG who have other autoimmune and neurological illnesses. All the serum specimens were tested for anti-AChR antibodies using 3 assays: in-house enzyme-linked immunosorbent assay (ELISA), the dot-blot assay, and commercial ELISA.ResultsIn-house ELISA, commercial ELISA, and dot-bl

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8

Elsaid, Mohamed Mohamed Anwar, Maria Terezinha Bahia, George Luiz Machado-Coelho, and Ricardo Wagner de Almeida Vitor. "Diagnosis of human toxoplasmosis by a dot enzyme-linked immunosorbent assay." Revista do Instituto de Medicina Tropical de São Paulo 37, no. 2 (1995): 117–22. http://dx.doi.org/10.1590/s0036-46651995000200005.

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A Dot enzyme-linked immunosorbent assay (Dot-ELISA) was standardized and evaluated for the serodiagnosis of human toxoplasmosis. Out of 538 serum samples tested by the immunofluorescence test for toxoplasmosis (IFAT-IgG) as reference test, 183 (34%) were positive at cut off 1:16 and 192 (36%) were positive for Dot-ELISA-IgG at cut-off 1:256. For Dot-ELISA, co-positivity was 0.94, co-negativity 0.94 and concordance 0.88 in relation to IFAT-IgG. These results suggest the usefulness of Dot-ELISA (cut-off titer of 1:256) for the serodiagnosis of human toxoplasmosis. The main advantage of this tech

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9

Kritsiriwuthinan, Kanyanan, Sumet Wajanarogana, Kantima Choosang, and Thitima Pimklang. "Comparison of Dot ELISA Using GroEL Recombinant Protein as an Antigen and an Indirect Hemagglutination Assay for Serodiagnosis of Melioidosis." Open Microbiology Journal 15, no. 1 (2021): 36–42. http://dx.doi.org/10.2174/1874285802115010036.

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Background: Melioidosis is a disease caused by the Burkholderia pseudomallei bacterium. The mortality rate of infected patients is quite high because the symptoms are similar to those of various diseases, making it difficult to diagnose clinically and preventing the immediate treatment with effective antibiotics that is required for the management of acute infections. To provide appropriate treatment, accurate and rapid diagnosis is required. Objective: The aims of this study were to develop Dot ELISA using purified GroEL B. pseudomallei recombinant protein as an antigen and to compare the new

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10

Guo, Mengmeng, Duo Qi, Jinxi Dong, et al. "Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees." Viruses 15, no. 1 (2023): 169. http://dx.doi.org/10.3390/v15010169.

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Plum pox virus (PPV) is a causal agent of the stone fruit tree sharka disease that often causes enormous economic losses. Due to its worldwide distribution and economic importance, rapid and reliable diagnostic technologies are becoming increasingly important for successful management of sharka disease. In this study, we have produced two super-sensitive and specific anti-PPV monoclonal antibodies (i.e., MAbs 13H4 and 4A11). Using these two MAbs, we have now developed a dot enzyme-linked immunosorbent assay (dot-ELISA) and a colloidal gold immunochromatographic strip (CGICS) assay. These two t

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