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Journal articles on the topic 'Dot-ELISA'

Author: Grafiati

Published: 4 June 2021

Last updated: 10 March 2023

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1

Gerbig, Donald G., Christopher J. Fenk, and Amy S. Goodhart. "The Dot Blot ELISA." American Biology Teacher 62, no. 8 (2000): 583–87. http://dx.doi.org/10.1662/0002-7685(2000)062[0583:tdbe]2.0.co;2.

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Khanal, Doj Raj, Eliza Ranjit, Sanjeev Kumar Adhikari, Bimal Ghemosu, Meera Prajapati, and Swoyam Prakash Shrestha. "Seroprevalence of mycoplasmosis in poultry of Bhaktapur." International Journal of Applied Sciences and Biotechnology 6, no. 1 (2018): 23–26. http://dx.doi.org/10.3126/ijasbt.v6i1.19103.

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Mycoplasmas are amongst the avian pathogens that causes chronic respiratory and joint diseases incurring a huge economic loss to poultry industry of Nepal. Among different species of Mycoplasmas, we investigate Mycoplasma gallisepticum-synoviae from the serum samples of the poultry using Enzyme Linked Immuno Sorbent Assay (ELISA) and dot-ELISA test of ImmunoComb. These tests rely on the antibodies present in the serum samples which binds to the pre-coated antigen in the ELISA/developing plates. A total of 92 sera samples were collected, of which 62 were from broiler and 30 were from layers. Of

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3

Bosompem, K. M., R. A. Masake, R. K. G. Assoku, E. A. Opiyo, and V. M. Nantulya. "Field evaluation of a dot-ELISA for the detection and differentiation of trypanosome species in infected tsetse flies (Glossinaspp.)." Parasitology 112, no. 2 (1996): 205–11. http://dx.doi.org/10.1017/s0031182000084778.

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SummaryA rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossinaspp.), was field tested alongside the standard fly dissection method on a ranch in south eastern Kenya. Of 104G. pallidipesdissected, 2 were found to be infected with trypanosomes in their midguts. By the dissection method the infecting trypanosome species could not be identified, as both flies had no salivary gland infections. However, using the dot-ELISA, the 2 flies were shown to be infected withTrypanosoma congolensein their midguts. The midguts of an add

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Requejo, Henry I. Z., Maria das Graças A. Alkmin, Regina G. Almeida, et al. "Dot-enzyme-linked immunosorbent assay (Dot-ELISA) for detection of pneumococcal polysaccharide antigens in pleural fluid effusion samples.: Comparison with bacterial culture, counterimmunoelectrophoresis and latex agglutination." Revista do Instituto de Medicina Tropical de São Paulo 36, no. 6 (1994): 531–37. http://dx.doi.org/10.1590/s0036-46651994000600010.

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A dot-enzyme-linked immunosorbent assay (Dot-ELISA) for pneumococcal antigen detection was standardized in view of the need for a rapid and accurate immunodiagnosis of acute pneumococcal pneumonia. A total of 442 pleural fluid effusion samples (PFES) from children with clinical and laboratory diagnoses of acute bacterial pneumonia, plus 38 control PFES from tuberculosis patients and 20 negative control serum samples from healthy children were evaluated by Dot-ELISA. The samples were previously treated with 0.1 M EDTA pH 7.5 at 90°C for 10 min and dotted on nitrocellulose membrane. Pneumococcal

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5

Macedo-Silva, A., S. F. C. Barbosa, M. G. A. Alkmin, A. J. Vaz, M. Shimokomaki, and A. Tenuta-Filho. "Hamburger meat identification by dot-ELISA." Meat Science 56, no. 2 (2000): 189–92. http://dx.doi.org/10.1016/s0309-1740(00)00039-5.

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6

Prakash, D., P. B. Parab, and R. N. Sharma. "Immunodiagnosis of dracunculiasis by dot-ELISA." Annals of Tropical Medicine & Parasitology 87, no. 2 (1993): 195–99. http://dx.doi.org/10.1080/00034983.1993.11812754.

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7

Bokoliya, Suresh, Shripad Patil, Madhu Nagappa, and Arun Taly. "A Simple, Rapid and Non-Radiolabeled Immune Assay to Detect Anti-AChR Antibodies in Myasthenia Gravis." Laboratory Medicine 50, no. 3 (2018): 229–35. http://dx.doi.org/10.1093/labmed/lmy038.

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AbstractObjectiveTo assess the practicality of dot-blot testing for rapid and sensitive detection of the antiacetylcholine receptor (anti-AChR) antibodies in myasthenia gravis (MG).MethodsIn this case-control study, we tested serum specimens of 85 patients with MG, 85 healthy control individuals, and 85 patients without MG who have other autoimmune and neurological illnesses. All the serum specimens were tested for anti-AChR antibodies using 3 assays: in-house enzyme-linked immunosorbent assay (ELISA), the dot-blot assay, and commercial ELISA.ResultsIn-house ELISA, commercial ELISA, and dot-bl

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8

Elsaid, Mohamed Mohamed Anwar, Maria Terezinha Bahia, George Luiz Machado-Coelho, and Ricardo Wagner de Almeida Vitor. "Diagnosis of human toxoplasmosis by a dot enzyme-linked immunosorbent assay." Revista do Instituto de Medicina Tropical de São Paulo 37, no. 2 (1995): 117–22. http://dx.doi.org/10.1590/s0036-46651995000200005.

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A Dot enzyme-linked immunosorbent assay (Dot-ELISA) was standardized and evaluated for the serodiagnosis of human toxoplasmosis. Out of 538 serum samples tested by the immunofluorescence test for toxoplasmosis (IFAT-IgG) as reference test, 183 (34%) were positive at cut off 1:16 and 192 (36%) were positive for Dot-ELISA-IgG at cut-off 1:256. For Dot-ELISA, co-positivity was 0.94, co-negativity 0.94 and concordance 0.88 in relation to IFAT-IgG. These results suggest the usefulness of Dot-ELISA (cut-off titer of 1:256) for the serodiagnosis of human toxoplasmosis. The main advantage of this tech

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9

Kritsiriwuthinan, Kanyanan, Sumet Wajanarogana, Kantima Choosang, and Thitima Pimklang. "Comparison of Dot ELISA Using GroEL Recombinant Protein as an Antigen and an Indirect Hemagglutination Assay for Serodiagnosis of Melioidosis." Open Microbiology Journal 15, no. 1 (2021): 36–42. http://dx.doi.org/10.2174/1874285802115010036.

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Background: Melioidosis is a disease caused by the Burkholderia pseudomallei bacterium. The mortality rate of infected patients is quite high because the symptoms are similar to those of various diseases, making it difficult to diagnose clinically and preventing the immediate treatment with effective antibiotics that is required for the management of acute infections. To provide appropriate treatment, accurate and rapid diagnosis is required. Objective: The aims of this study were to develop Dot ELISA using purified GroEL B. pseudomallei recombinant protein as an antigen and to compare the new

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10

Guo, Mengmeng, Duo Qi, Jinxi Dong, et al. "Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees." Viruses 15, no. 1 (2023): 169. http://dx.doi.org/10.3390/v15010169.

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Plum pox virus (PPV) is a causal agent of the stone fruit tree sharka disease that often causes enormous economic losses. Due to its worldwide distribution and economic importance, rapid and reliable diagnostic technologies are becoming increasingly important for successful management of sharka disease. In this study, we have produced two super-sensitive and specific anti-PPV monoclonal antibodies (i.e., MAbs 13H4 and 4A11). Using these two MAbs, we have now developed a dot enzyme-linked immunosorbent assay (dot-ELISA) and a colloidal gold immunochromatographic strip (CGICS) assay. These two t

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11

Eamsobhana, P., A. Yoolek, P. Punthuprapasa, and S. Suvouttho. "A dot-blot ELISA comparable to immunoblot for the specific diagnosis of human parastrongyliasis." Journal of Helminthology 78, no. 4 (2004): 287–91. http://dx.doi.org/10.1079/joh2004257.

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AbstractA dot-blot enzyme-linked immunosorbent assay (dot-blot ELISA) using an electroeluted 31-kDa glycoprotein from adult worms ofParastrongylus cantonensisas the specific antigen was evaluated for the immunological diagnosis of patients infected withP. cantonensis. The sensitivity and specificity for the detection of serum antibody toP. cantonensisin dot-blot ELISA were both 100%, as determined with serum samples of tenP. cantonensis-infected patients, 60 patients with other related parasitic infections, and 20 uninfected controls. The test was as sensitive and specific as the immunoblot te

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12

Camargo, Eide Dias, Paulo Mutuko Nakamura, Adelaide José Vaz, Marcos Vinícius da Silva, Pedro Paulo Chieffi, and Elisabete Ourique de Melo. "Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA." Revista do Instituto de Medicina Tropical de São Paulo 34, no. 1 (1992): 55–60. http://dx.doi.org/10.1590/s0036-46651992000100010.

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The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but

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13

Jarvi, Susan Irene, Stefano Quarta, Praphathip Eamsobhana, et al. "Human exposure to Angiostrongylus cantonensis on east Hawaii Island." Journal of Immunology 198, no. 1_Supplement (2017): 57.16. http://dx.doi.org/10.4049/jimmunol.198.supp.57.16.

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Abstract A study was completed to quantify exposure to Angiostrongylus cantonensis among 435 human volunteers from east Hawaii Island. Volunteers completed a questionnaire and provided blood samples (at Puna Community Medical Center and Clinical Labs of Hawaii). Serum was screened by indirect ELISA using crude antigen isolated from adult A. cantonensis harvested from wild Hawaiian rats, and 28% tested positive. Of volunteers who defined themselves as definitively diagnosed by a clinician (n = 15), 67% were positive by ELISA and reported being infected in 2004 and later. Of those self-reported

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14

Pinto, Pedro Luiz Silva, Herminia Y. Kanamura, Rita Maria Silva, Cristina Renata Nardoto Rossi, Heitor Franco de Andrade Jr., and Vicente Amato Neto. "Dot-ELISA for the detection of IgM and IgG antibodies to Schistosoma mansoni worm and egg antigens, associated with egg excretion by patients." Revista do Instituto de Medicina Tropical de São Paulo 37, no. 2 (1995): 109–15. http://dx.doi.org/10.1590/s0036-46651995000200004.

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Human schistosomiasis, caused by Schistosoma mansoni, is highly prevalent in Brazil and usually diagnosed by time consuming stool analysis. Serological tests are of limited use in this disease, mainly for epidemiological studies, showing no discrimination between previous contact with the parasite and active infections. In the present study, we standardized and compared a Dot-ELISA for IgM and IgG antibodies against S. mansoni antigens from eggs and worms with a routine IgG and IgM immunofluorescence assay using similar antigens, in the study of sera from 27 patients who had quantified egg sto

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15

Leite, Olavo Henrique Munhoz. "Diagnosis of pulmonary tuberculosis using DOT-ELISA and ELISA immunoperoxidase techniques." Revista da Sociedade Brasileira de Medicina Tropical 23, no. 4 (1990): 241–42. http://dx.doi.org/10.1590/s0037-86821990000400014.

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16

Montenegro, Silvia Maria Lucena, Joanne D'arc Bezerra da Silva, Maria Edileuza Felinto de Brito, and Luiz Bezerra de Carvalho Junior. "Dot enzyme-linked immunosorbent assay (dot-ELISA) for schistosomiasis diagnosis using dacron as solid-phase." Revista da Sociedade Brasileira de Medicina Tropical 32, no. 2 (1999): 139–43. http://dx.doi.org/10.1590/s0037-86821999000200004.

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Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF). Titration of sera from 18 schistosomiasis patients against soluble worm antigen preparation (SWAP) was carried out and sera from healthy individuals from non-endemic areas were used as controls. The IMF was less sensitive than the dot-ELISAs, although the difference was not statistically significant (p > 0.05). The dot-ELISA based on nitrocellulose was as sensitive as that using dacron. Stability did not differ

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17

Zhang, Can, Xiaoxiao Wu, Dongyang Li, et al. "Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid." Analytical Methods 13, no. 14 (2021): 1757–65. http://dx.doi.org/10.1039/d1ay00129a.

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Flow-through dot ELISA and gold nanoparticle lateral-flow immunoassay based on nanobodies were developed for detecting 3-PBA. The sensitivity of nanobody-based flow-through dot ELISA is 10-fold higher than that of gold nanoparticle lateral-flow immunoassay.

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18

Vaz de Lima, Lourdes R. A., Sumie Hoshino-Shimizu, Vanda A. U. F. de Souza, et al. "Measles serodiagnosis: standardization and evaluation of a dot-ELISA." Revista do Instituto de Medicina Tropical de São Paulo 36, no. 2 (1994): 139–47. http://dx.doi.org/10.1590/s0036-46651994000200008.

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A Dot-ELISA using a measles virus (MV) antigen obtained by sodium deoxycholate treatment was standardized and evaluated for IgM and IgG antibody detection in measles patients and measles-vaccinated subjects. A total of 192 serum samples were studied, comprising 47 from patients with acute and convalescent measles, 55 from 9-month old children prior to measles vaccination and 41 from children of the same age after vaccination, and 49 from patients with unrelated diseases. The diagnostic performances of the IgG Dot-ELISA and IgG immuno fluorescence test (IFT) were found to be close, varying from

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19

Biswas, Rakhi, S. C. Parija, and S. K. Narayan. "Dot-ELISA for the diagnosis of neurocysticercosis." Revista do Instituto de Medicina Tropical de São Paulo 46, no. 5 (2004): 249–52. http://dx.doi.org/10.1590/s0036-46652004000500003.

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The aim of the present study was to standardize and evaluate dot-Enzyme linked immunosorbent assay (Dot-ELISA), a simple and rapid test for the detection of cysticercus antibodies in the serum for the diagnosis of neurocysticercosis (NCC). The antigen used in the study was a complete homogenate of Cysticercus cellulosae cysts obtained from infected pigs and dotted on to nitrocellulose membrane. Test sera were collected from the patients of NCC, and control sera from patients with other diseases and healthy students and blood donors of the Jawaharlal Institute of Postgraduate Medical Education

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20

El-Ameer, A. M., M. M. Ramadan, and I. R. Shalash. "Diagnosis of Echinococcosis (Hydatidosis) Using Dot-ELISA." Egyptian Journal of Hospital Medicine 64 (July 2016): 304–10. http://dx.doi.org/10.12816/0029022.

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21

Janitschke, Klaus, Astrid Reinhold, and Liv Bode. "Nitrocellulose dot-ELISA for serodiagnosis of schistosomiasis." Transactions of the Royal Society of Tropical Medicine and Hygiene 81, no. 6 (1987): 956–58. http://dx.doi.org/10.1016/0035-9203(87)90363-4.

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22

S. Guimarães, M. Carolina, and Beatriz J. Celeste. "Performance indexes of a dot-enzime-linked immunosorbent assay (dot-ELISA) and an ezyme-linked immunosorbent assay (IgG-ELISA) for field surveys of new world leishmaniasis." Revista do Instituto de Medicina Tropical de São Paulo 33, no. 5 (1991): 385–89. http://dx.doi.org/10.1590/s0036-46651991000500008.

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Diagnostic performance indexes of sensitivity, specificity, positive predictive value and efficiency were determined for dot-ELISA and IgG-ELISA tests in 340 leishmaniasis sera. Sensitivity of the dot-ELISA was significantly lower than IgG-ELISA's; the two tests had indexes of specificity and positive predictive value of the same magnitude. Seventy-eight sera gave a negative dot-ELISA test result and a positive IgG-ELISA test result. When sera were classified according to different criteria as how to interpret this diversity, the kappa statistic did not corroborate the classification indicatin

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23

Wang, Liping, Guoping Wang, Ni Hong, Rongrong Tang, Xiaoyun Deng, and Hong Zhang. "Effect of Thermotherapy on Elimination of Apple Stem Grooving Virus and Apple Chlorotic Leaf Spot Virus for In vitro-cultured Pear Shoot Tips." HortScience 41, no. 3 (2006): 729–32. http://dx.doi.org/10.21273/hortsci.41.3.729.

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Apple stem grooving virus (ASGV) and apple chlorotic leaf spot virus (ACLSV) are two major viruses of pear. In this study, in vitro thermotherapy was carried out at 37°C for 25, 30 and 35 days followed by subculturing of meristem tips of different sizes to eliminate ASGV and ACLSV from pear plants. Virus titers in heat-treated shoot tips were evaluated by ELISA testing of regenerated plants. Results showed that thermotherapy for 35 days significantly decreased the titer of ASGV and ACLSV in cultures regenerated from tips of main and axillary shoots, especially in those from explants 1 mm in le

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Chan, S. W., and Ronald C. Ko. "Comparison between standard ELISA and dot-ELISA for serodiagnosis of human trichinosis." Transactions of the Royal Society of Tropical Medicine and Hygiene 82, no. 6 (1988): 892–94. http://dx.doi.org/10.1016/0035-9203(88)90030-2.

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25

He, Wanqin, Deqing Huang, Jiayu Wu, et al. "Three Highly Sensitive and High-Throughput Serological Approaches for Detecting Dickeya dadantii in Sweet Potato." Plant Disease 105, no. 4 (2021): 832–39. http://dx.doi.org/10.1094/pdis-07-20-1551-re.

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Sweet potato stem and root rot is an important bacterial disease and often causes serious economic losses to sweet potato. Development of rapid and sensitive detection methods is crucial for diagnosis and management of this disease in field. Here, we report the production of four hybridoma cell lines (25C4, 16C10, 9B1, and 9H10) using Dickeya dadantii strain FY1710 as an immunogen. Monoclonal antibodies (MAbs) produced by these four hybridoma cell lines were highly specific and sensitive for D. dadantii detection. Indirect enzyme-linked immunosorbent assay (indirect-ELISA) results showed that

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Téllez-Girón, Edmundo, Pilar Alvarez, Leticia Dufour, Martha C. Ramos, and Margarita Montante. "Detection of Cysticercus cellulosae Antigens in Cerebrospinal Fluid by Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) and Standard ELISA." American Journal of Tropical Medicine and Hygiene 37, no. 1 (1987): 169–73. http://dx.doi.org/10.4269/ajtmh.1987.37.169.

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27

Swarna, S. R., and Subhash Chandra Parija. "Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis." Revista do Instituto de Medicina Tropical de São Paulo 50, no. 4 (2008): 233–36. http://dx.doi.org/10.1590/s0036-46652008000400009.

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The aim of the present study is to evaluate cyst wall and protoscolex as an alternate source of antigen in serodiagnosis of cystic echinococcosis (CE). A total of 90 blood samples, 30 each of confirmed CE cases, disease controls and healthy controls were collected. Dot-ELISA using cyst wall, protoscolex and cyst fluid were used to demonstrate anti-hydatid antibodies. The sensitivity of Dot-ELISA using cyst wall, protoscolex and cyst fluid was 96.66%, 86.66% and 93.33% respectively and the specificity of the assay was 70% for Dot-ELISA using cyst fluid, protoscolex and cyst wall antigens. Resul

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Hassan, Sarah, Vineeta Khare, Shadma Yaqool, Syed Abid Asghar, Mastan Singh, and Zeba Siddiqi. "Comparative study between WIDAL and DOT ELISA in the diagnosis of Typhoid fever." Asian Journal of Medical Sciences 12, no. 4 (2021): 81–85. http://dx.doi.org/10.3126/ajms.v12i4.33192.

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Background: Typhoid fever, also known as enteric fever, is a communicable disease, found only in man and occurs due to systemic infection mainly by Salmonella typhi organisms. Blood culture is regarded as the gold standard for diagnosis and carry 70-75% diagnostic yield in the first week of illness. Aims and Objective: To compare the sensitivity and specificity of Widal test and dot ELISA with blood culture in the early diagnosis of Typhoid fever. Materials and Methods: A Cross-Sectional study was carried out in the Department of Microbiology, Era’s Lucknow Medical College and Hosp

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Mishra, Archana, Ajay Kumar Sharma, and Vandana Tewari. "Intact parasite-antigen ELISA test: a new dimension for serodiagnosis of Amoebiasis." Journal of Applied and Natural Science 10, no. 3 (2018): 976–80. http://dx.doi.org/10.31018/jans.v10i3.1835.

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An Intact Parasite Antigen ELISA (IPA-ELISA) has been developed for detection of circulating antibodies against Entamoeba. histolytica. Axenically grown trophozoites of E.histolytica (NIH-200) after glutaraldehyde treatment, are used as Intact Parasite Antigen (IPA) as well biological active and imperishable base. Antigen over the surface of treated cells were allowed to interact with the antibody molecules of the test sera. The techniques of Plate, Dot-and IPA-ELISA are compared of their merits for clinical and epidemiological survey of amoebiasis. IPA-ELISA was found to be more sensitive (96

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Fisa, R., M. Gállego, C. Riera, et al. "Serologic Diagnosis of Canine Leishmaniasis by Dot-ELISA." Journal of Veterinary Diagnostic Investigation 9, no. 1 (1997): 50–55. http://dx.doi.org/10.1177/104063879700900109.

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A dot-enzyme-linked immunosorbent assay (ELISA) using protein A-peroxidase was evaluated as a diagnostic test for canine leishmaniasis. The test results were in agreement with parasitologic diagnosis and indirect immunofluorescence assay results. The sensitivity of the test calculated on 31 dogs with positive parasitologic examination was 90% when a titer of 1/800 was established as a cutoff and 100% when a titer of 1/400 was established. The specificity calculated on the canine population from nonendemic areas was 100% when both titers were established. Nevertheless, in endemic areas titers n

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Taher, Eman E., Eman M. H. Méabed, Dina M. H. El Akkad, Nancy O. Kamel, and Maha A. Sabry. "Modified dot-ELISA for diagnosis of human trichinellosis." Experimental Parasitology 177 (June 2017): 40–46. http://dx.doi.org/10.1016/j.exppara.2017.04.002.

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Kalvatchev, Z., and E. Alexandrov. "Dot-Elisa for Rapid Detection of Antirickettsial Antibodies." Biotechnology & Biotechnological Equipment 6, no. 2 (1992): 41–42. http://dx.doi.org/10.1080/13102818.1992.10818654.

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33

Weidemann, Hans-L. "Rapid detection of potato viruses by dot-ELISA." Potato Research 31, no. 3 (1988): 485–92. http://dx.doi.org/10.1007/bf02357886.

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Pappas, Michael G. "Recent applications of the Dot-ELISA in immunoparasitology." Veterinary Parasitology 29, no. 2-3 (1988): 105–29. http://dx.doi.org/10.1016/0304-4017(88)90120-3.

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35

Montenegro, Silvia M. L., Alzira M. P. de Almeida, Alexandre B. de Carvalho, and Luiz B. de Carvalho Júnior. "The use of dacron plates for DOT enzyme-linked immunosorbent assay (DOT-ELISA)." Memórias do Instituto Oswaldo Cruz 86, no. 4 (1991): 461–65. http://dx.doi.org/10.1590/s0074-02761991000400016.

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Montenegro, Silvia M. L., Alzira M. P. de Almeida, and Luiz B. Carvalho Júnior. "Standardization of the dot enzyme-lynked immunosorbent assay (dot-ELISA) for experimental plague." Memórias do Instituto Oswaldo Cruz 88, no. 1 (1993): 119–23. http://dx.doi.org/10.1590/s0074-02761993000100018.

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37

Srivastava, Lakshmi, and Vijay K. Singh. "Diagnosis of Indian kala-azar by dot enzyme-linked immunosorbent assay (dot—ELISA)." Annals of Tropical Medicine & Parasitology 82, no. 4 (1988): 331–34. http://dx.doi.org/10.1080/00034983.1988.11812254.

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38

Bosompem, K. M., R. K. G. Assoku, and V. M. Nantulya. "Differentiation between culture-derived insect stages ofT. brucei,T. vivax, T. congolenseandT. simiaeusing a monoclonal antibody-based dot–ELISA." Parasitology 112, no. 1 (1996): 59–66. http://dx.doi.org/10.1017/s0031182000065070.

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SUMMARYA sensitive and specific nitrocellulose (NC) membrane-based dot–ELISA, utilizing a panel of monoclonal antibodies (mAbs), was developed for differentiation betweenin vitro-derivedprocyclic forms ofTrypanosoma brucei,T. congolenseandT. simiae, and epimastigotes ofT. vivax. Trypanosomes in suspension were applied onto NC membrane in dots and probed with unlabelled trypanosome species-specific mAbs. Bound mAb was revealed by enzyme labelled anti-mouse IgG and precipitable chromogenic substrate. The assay detected the aforementioned trypanosome species in both single and artificially mixed

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Cinthujah B, Cinthujah B., Amudha VP Amudha VP, and Sucilathangam G. Sucilathangam G. "Comparative Study of Widal and Dot Elisa in the Diagnosis of Typhoid Fever." International Journal of Scientific Research 3, no. 4 (2012): 303–4. http://dx.doi.org/10.15373/22778179/apr2014/104.

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40

Branch, Songee L., and Paul N. Levett. "Evaluation of Four Methods for Detection of Immunoglobulin M Antibodies to Dengue Virus." Clinical Diagnostic Laboratory Immunology 6, no. 4 (1999): 555–57. http://dx.doi.org/10.1128/cdli.6.4.555-557.1999.

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ABSTRACT Dengue has become hyperendemic in many islands of the Caribbean region. The performance in a diagnostic laboratory of four commercial assays for detection of immunoglobulin M (IgM) antibodies was evaluated. Sera from 62 patients with dengue virus infection were studied. These included 18 patients from whom dengue virus type 2 was isolated in a 1997 outbreak (specimens collected a mean of 14 days after onset of symptoms), 8 patients with dengue hemorrhagic fever (mean time after onset, 11 days), and 36 patients in whom dengue was previously confirmed by serology (mean time after onset,

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URWIN, R., I. M. FEAVERS, D. M. JONES, M. C. J. MAIDEN, and A. J. FOX. "Molecular variation of meningococcal serotype 4 antigen genes." Epidemiology and Infection 121, no. 1 (1998): 95–101. http://dx.doi.org/10.1017/s0950268898008942.

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Changes in the frequency of serogroup B non serotypable (B[ratio ]NT) meningococci isolated in England and Wales were investigated by T-track fingerprint analysis, DNA nucleotide sequence determination, and serotyping by whole cell ELISA and dot blot assay. Seventy-three per cent of the isolates designated as B[ratio ]NT by the Meningococcal Reference Unit (MRU) dot blot assay during 1993–4, expressed variants of the serotyping antigen, PorB, that were serotype 4 by whole cell ELISA. T-track fingerprint patterns of these and other ‘serotype 4’ isolates revealed five distinct porB alleles which

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Vera-Cabrera, Lucio, Adrian Rendon, Manuel Diaz-Rodriguez, Vera Handzel, and Adalbert Laszlo. "Dot Blot Assay for Detection of Antidiacyltrehalose Antibodies in Tuberculous Patients." Clinical Diagnostic Laboratory Immunology 6, no. 5 (1999): 686–89. http://dx.doi.org/10.1128/cdli.6.5.686-689.1999.

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ABSTRACT A simple dot blot test with diacyltrehalose (DAT) as the antigen was developed to detect anti-DAT antibodies in tuberculous patients. To enhance antigen-antibody reaction detection, rabbit serum raised against human immunoglobulins was used prior to incubation with a protein A-colloidal gold complex. With the dot blot system, it was possible to obtain a sensitivity similar to that of enzyme-linked immunosorbent assay (ELISA) and a specificity of 97.14%, versus a specificity of 94.29% by the ELISA. We conclude that this simple and fast assay could be used in places where ELISA equipmen

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Melo, Fabio G., and Claudio L. Rossi. "Dot-enzyme-linked immunosorbent assay (dot-ELISA) for evaluating IgG antibody avidity in toxoplasmosis." Revista do Instituto de Medicina Tropical de São Paulo 39, no. 4 (1997): 235–336. http://dx.doi.org/10.1590/s0036-46651997000400011.

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Shaheen, Hind I., Karim A. Kamal, Zoheir Farid, Noshy Mansour, Fouad N. Boctor, and James N. Woody. "Dot-Enzyme-Linked Immunosorbent Assay (Dot-ELISA) for the Rapid Diagnosis of Human Fascioliasis." Journal of Parasitology 75, no. 4 (1989): 549. http://dx.doi.org/10.2307/3282904.

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Roldán, William H., Yrma A. Espinoza, Pedro E. Huapaya, Alina F. Huiza, Carlos R. Sevilla, and Susana Jiménez. "Frequency of human toxocariasis in a rural population from Cajamarca, Peru determined by DOT-ELISA test." Revista do Instituto de Medicina Tropical de São Paulo 51, no. 2 (2009): 67–71. http://dx.doi.org/10.1590/s0036-46652009000200002.

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The aim of this study was to estimate the frequency of human toxocariasis in Cauday district, Cajamarca, Peru, using a dot-ELISA test. From June to October 2005, a total of 256 adult subjects were studied. Blood samples were collected for serology by a dot-ELISA test and for hematological examination. Parasitological examination was also carried out in stool samples to check cross-reactions in the dot-ELISA. The frequency observed was 44.92%, with a significant higher proportion of positivity in male subjects. From subjects with positive serology, 45.6% had respiratory symptoms, 40.44% abdomin

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Xun, Yi Ping, Hai Yan, Jia Liu, Li Li Shi, Peng Chen, and Hong Wu Du. "Development of a High-Throughput Immunoassay for Rapid Detection of Multiple Antibiotic Residues in Cosmetics." Advanced Materials Research 1073-1076 (December 2014): 357–61. http://dx.doi.org/10.4028/www.scientific.net/amr.1073-1076.357.

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Antibiotic residues in cosmetics can cause seriously healthy problems such as sensitization, systemic toxicity, or even death. The developments of rapid methods for multiple antibiotics detection are needed to conveniently detect out the illegal additives. In this study, a simple, cost-effective and time-saving immunoassay, multi-analyte dot enzyme-linked immunosorbent assay (Multi-Dot-ELISA), for the simultaneous detection of three most common abused antibiotics (CPFX, TC, and SMD) was successfully designed and developed. Based on the competitive Multi-Dot-ELISA, real samples from 5 of 15 (33

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ABOU-ELHAKAM, HANY, ALYAA FARID, NOHA MAHANA, IBRAHIUM BAUIOMY, and AZZA ELAMEER. "DOT-ELISA AS A FIELD TEST FOR HYDATID DIAGNOSIS." Journal of the Egyptian Society of Parasitology 46, no. 2 (2016): 441–52. http://dx.doi.org/10.21608/jesp.2016.88731.

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Yamaura, Hisashi, Ken Kikuchi, Ichiro Itoda, Kyoichi Totsuka, Kunioki Araki, and Takatoshi Kobayakawa. "Evaluation of dot-ELISA for serological diagnosis of amebiasis." Journal of Infection and Chemotherapy 9, no. 1 (2003): 25–29. http://dx.doi.org/10.1007/s10156-002-0226-2.

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Abou-Elhakam, Hany M., Alyaa A. Farid, and Noha A. Mahana. "Dot-Elisa as a Field Test for Hydatid Diagnosis." Journal of the Egyptian Society of Parasitology 46, no. 2 (2016): 441–52. http://dx.doi.org/10.12816/0031650.

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Lissaldo, Ana Maria, Sumie Hoshino-Shimizu, Eufrosina Setsu Umezawa, and Anna Maria Simonsen Stolf. "Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA." Revista do Instituto de Medicina Tropical de São Paulo 36, no. 2 (1994): 163–66. http://dx.doi.org/10.1590/s0036-46651994000200012.

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The alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) was assessed in dot-ELISA for the diagnosis of Chagas' disease. Serum samples (355) from chagasic and non-chagasic patients were studied, and IgG antibodies to ASEA were found in all patients with chronic Chagas' disease. In non-chagasic patients 95.6% were negative, except for those with leishmaniasis (visceral and mucocutaneous), and some patients from control group reacted in low titers. The data indicate that dot-ELISA using ASEA is suitable for seroepidemiologic surveys to be employed in endemic areas for Chagas' disease.

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