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Journal articles on the topic 'DPPH assay'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Sharma, Om P., and Tej K. Bhat. "DPPH antioxidant assay revisited." Food Chemistry 113, no. 4 (2009): 1202–5. http://dx.doi.org/10.1016/j.foodchem.2008.08.008.

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2

Gulcin, İlhami, and Saleh H. Alwasel. "DPPH Radical Scavenging Assay." Processes 11, no. 8 (2023): 2248. http://dx.doi.org/10.3390/pr11082248.

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Today, there is an increasing interest in antioxidants, especially to prevent the known harmful effects of free radicals in human metabolism and their deterioration during processing and storage of fatty foods. In both cases, natural-source antioxidants are preferred over synthetic antioxidants. So, there has been a parallel increase in the use of assays to estimate antioxidant efficacy in human metabolism and food systems. Today, there are many bioanalytical methods that measure the antioxidant effect. Of these, the 1,1-diphenyl-2-picrylhydrazil (DPPH) removing assay is the most putative, pop

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Platzer, Melanie, Sandra Kiese, Thomas Herfellner, Ute Schweiggert-Weisz, Oliver Miesbauer, and Peter Eisner. "Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays." Molecules 26, no. 5 (2021): 1244. http://dx.doi.org/10.3390/molecules26051244.

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Numerous assays were developed to measure the antioxidant activity, but each has limitations and the results obtained by different methods are not always comparable. Popular examples are the DPPH and ABTS assay. Our aim was to study similarities and differences of these two assay regarding the measured antioxidant potentials of 24 phenolic compounds using the same measurement and evaluation methods. This should allow conclusions to be drawn as to whether one of the assays is more suitable for measuring specific subgroups like phenolic acids, flavonols, flavanones, dihydrochalcones or flavanols

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4

Jafri, Syed Anis Ali, Zafar Mahmood Khalid, Muhammad Zakryya Khan, and NaqeebUllah Jogezai. "Evaluation of phytochemical and antioxidant potential of various extracts from traditionally used medicinal plants of Pakistan." Open Chemistry 20, no. 1 (2022): 1337–56. http://dx.doi.org/10.1515/chem-2022-0242.

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Abstract The antioxidant potential of various extracts was evaluated using different antioxidant assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, and 2,2-azinobis-ethylbenzothiozoline-6-sulphonic acid (ABTS) using UV spectrophotometer. The highest absorbance was observed in ethanolic extracts (EEs) of Euphrasia stricta 71.92 ± 1.22%, 65.77 ± 1.38%, and 67.88 ± 0.74%, followed by methanolic extracts (MEs) 70.14 ± 0.82%, 64.84 ± 0.74%, and 65.48 ± 1.40% for DPPH assay (517 nm), FRAP assay (700 nm), and ABTS assay (734 nm), respectively. T

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Tayade, Sunil, Kamlakar More, and Prashant Gawande. "Antioxidative properties of Mucuna nivea (Roxb.) DC by DPPH Assay." SSR Institute of International Journal of Life Sciences 6, no. 6 (2020): 2687–93. http://dx.doi.org/10.21276/ssr-iijls.2020.6.6.3.

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6

Yamauchi, Moeka, Yukino Kitamura, Haruka Nagano, Junya Kawatsu, and Hiroaki Gotoh. "DPPH Measurements and Structure—Activity Relationship Studies on the Antioxidant Capacity of Phenols." Antioxidants 13, no. 3 (2024): 309. http://dx.doi.org/10.3390/antiox13030309.

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The consumption of foods that are high in antioxidant capacity is believed to contribute to good health. Moreover, the addition of highly antioxidant compounds to foods is believed to prevent food deterioration. Among the known antioxidants in food, phenols have been identified as the primary antioxidants. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay is a simple, inexpensive, and rapid method widely used to evaluate the antioxidant capacity. Although the results of the DPPH assay depend on conditions such as the reaction time and concentration, the experimental conditions have not been stand

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7

Frezzini, Maria Agostina, Federica Castellani, Nayma De Francesco, Martina Ristorini, and Silvia Canepari. "Application of DPPH Assay for Assessment of Particulate Matter Reducing Properties." Atmosphere 10, no. 12 (2019): 816. http://dx.doi.org/10.3390/atmos10120816.

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Different acellular assays were developed to measure particulate matter’s (PM) oxidative potential (OP), a metric used to predict the ability of PM in generating oxidative stress in living organisms. However, there are still fundamental open issues regarding the complex redox equilibria among the involved species which could include reducing compounds. The aim of this study was the pilot application of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to PM in order to evaluate the presence of reducing species. The assay, commonly applied to biological matrices, was adapted to PM and showed good

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Alam, M. Ashraful, Abdul Ghani, Nusrat Subhan, et al. "Antioxidant and Membrane Stabilizing Properties of the Flowering Tops of Anthocephalus Cadamba." Natural Product Communications 3, no. 1 (2008): 1934578X0800300. http://dx.doi.org/10.1177/1934578x0800300114.

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The hydroethanolic extract of Anthocephalus cadamba displayed remarkable antioxidative potential in the 1,1-diphenyl-2-picrylhydrazyl (DPPH), the hydrogen peroxide, the nitric oxide scavenging, the reducing power, the total antioxidant capacity, the lipid peroxidation inhibition (thiobarbituric acid-reactive substances production), and the RBC membrane stabilization assays. While in the DPPH assay the IC50 value of the extract was 146.5 μg/mL, it was 24.8 μg/mL in the nitric oxide scavenging assay.

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9

Zahra, N., MK Saeed, S. Nawaz, and E. Gulzar. "Aloe vera cookies preparation, nutritional aspects, DPPH assay and physicochemical assay." Bangladesh Journal of Scientific and Industrial Research 57, no. 2 (2022): 117–22. http://dx.doi.org/10.3329/bjsir.v57i2.60408.

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Aloe vera has been widely used for various therapeutic purposes over the millennia. It is becoming increasingly popular due to its antiviral and antibacterial qualities. For many years, aloe vera has been employed in biomedical research. It's a fantastic way to boost the nutritional value of any food product. This research aims to expand on the nutritional value of cookies by including Aloe vera gel. It would focus on the substance's biological and pharmacological characteristics. The nutritional quality of aloe vera cookies was evaluated after being manufactured with all-natural, chemical-fre

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Leki, Karol Giovani Battista, Yahya Febrianto, and Yithro Serang. "Evaluation of Antioxidant Activity in Solanum Ferox (Through DPPH and FRAP Assays)." JURNAL INFO KESEHATAN 23, no. 2 (2025): 309–17. https://doi.org/10.31965/infokes.vol23.iss2.1743.

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The antioxidant properties of natural compounds have garnered significant attention for their potential therapeutic applications. Solanum ferox, a plant traditionally used in several countries for medicinal purposes, has been identified as possessing potential antioxidant properties. However, the extent and efficacy of its antioxidant capacity have not been thoroughly examined, creating a gap in understanding its effectiveness compared to established antioxidants like ascorbic acid. This study aims to evaluate the antioxidant capacity of Solanum ferox using DPPH and FRAP assays across concentr

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11

Shekade, Pratiksha Prabhakar, Sumedh Sunil Chavan, and Arvind Shankarrao Dhabe. "Antioxidant Potential of Arogyavardhini Vati Extract by DPPH Assay." Journal of Drug Delivery and Therapeutics 15, no. 7 (2025): 25–28. https://doi.org/10.22270/jddt.v15i7.7254.

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Arogyavardhini Vati, an Ayurvedic medicine, was commonly used during the COVID-19 pandemic for its immunomodulatory and therapeutic properties. The DPPH radical scavenging assay in this study revealed its antioxidant potential. This Ayurvedic medicine demonstrated considerable antioxidant capability with 78.04% DPPH scavenging activity. These findings support its traditional use and suggest the need for further investigation. Keywords: Arogyavardhini Vati, DPPH assay, antioxidant activity, ethanol extract, COVID-19, Soxhlet’s extraction, etc.

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Darshika, Acharya Meenakshi Vaidya*. "In-vitro antioxidant activity of hydroalcoholic extract of leaves of Hydnocarpus pentandrus (Buch. - Ham) Oken." International Journal of Pharmaceutical Sciences 2, no. 11 (2024): 167–72. https://doi.org/10.5281/zenodo.14030494.

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Cellular damage can arise from the reactions of free radicals with membrane lipids, nucleic acids, proteins, enzymes, and other micro molecules. Free radical-induced cell damage seems to be a primary factor in the aging process and degenerative diseases, including but not limited to cancer, heart disease, cataracts, liver disorders, diabetes mellitus, inflammation, and renal failure. Naturally, the body produces free radicals, and antioxidants scavenge them to shield the body from harmful consequences. This dynamic equilibrium exists between the two. It's possible that there aren't enough anti

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Pugalenthi, M., M. Pradheeba, S. Vishnu Kumar, G. Vasukipriyadharshini, S. Swathi, and G. Divya Bharathi. "UNVEILING THE PHYTOCHEMICAL PROFILE, SECONDARY METABOLITE QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF CLEMATIS WIGHTIANA WALL. EX WIGHT and ARN." Journal of Advanced Scientific Research 13, no. 03 (2022): 70–77. http://dx.doi.org/10.55218/jasr.202213312.

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Traditionally, the leaves of Clematis wightiana have been used in the treatment of rheumatism, indigestion, headaches, varicose veins, bone problems, nasal congestion and sinus. The present study was conducted to evaluate the phytochemical profile, quantification of secondary metabolites and free radical scavenging capacity of C. wightiana leaf. The total phenolic, tannin and flavanoid content of C. wightiana leaves were quantified and were found to be higher in the ethyl acetate extract. Subsequently, the extracts were subjected to appraise their antioxidant capacity by availing various in vi

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14

SIRIVIBULKOVIT, Kitima, Souksanh NOUANTHAVONG, and Yupaporn SAMEENOI. "Paper-based DPPH Assay for Antioxidant Activity Analysis." Analytical Sciences 34, no. 7 (2018): 795–800. http://dx.doi.org/10.2116/analsci.18p014.

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15

Cheng, Zhihong, Jeffrey Moore, and Liangli (Lucy) Yu. "High-Throughput Relative DPPH Radical Scavenging Capacity Assay." Journal of Agricultural and Food Chemistry 54, no. 20 (2006): 7429–36. http://dx.doi.org/10.1021/jf0611668.

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16

Sukrasno, Sukrasno, Slamet Tuty, and Irda Fidrianny. "ANTIOXIDANT EVALUATION AND PHYTOCHEMICAL CONTENT OF VARIOUS RICE BRAN EXTRACTS OF THREE VARIETIES RICE FROM SEMARANG-CENTRAL JAVA, INDONESIA." Asian Journal of Pharmaceutical and Clinical Research 10, no. 6 (2017): 377. http://dx.doi.org/10.22159/ajpcr.2017.v10i6.16565.

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Objectives: The objectives of this research were to evaluate antioxidant activity from different polarities rice bran extract of three varieties of rice using two methods of antioxidant testing which were FRAP (Ferric Reducing Antioxidant Power) and DPPH (2,2-diphenyl-1-picrylhydrazyl), and correlation of total phenolic, flavonoid and carotenoid content with their EC50 of FRAP and IC50 of DPPH antioxidant activities. Methods: Extraction was conducted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Determination of total phenolic, flavonoid and

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Sy, Biranty, Stéphanie Krisa, Tristan Richard та Arnaud Courtois. "Resveratrol, ε-Viniferin, and Vitisin B from Vine: Comparison of Their In Vitro Antioxidant Activities and Study of Their Interactions". Molecules 28, № 22 (2023): 7521. http://dx.doi.org/10.3390/molecules28227521.

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The control of oxidative stress with natural active substances could limit the development of numerous pathologies. Our objective was to study the antiradical effects of resveratrol (RSV), ε-viniferin (VNF), and vitisin B (VB) alone or in combination, and those of a standardized stilbene-enriched vine extract (SSVE). In the DPPH-, FRAP-, and NO-scavenging assays, RSV presented the highest activity with an IC50 of 81.92 ± 9.17, 13.36 ± 0.91, and 200.68 ± 15.40 µM, respectively. All binary combinations resulted in additive interactions in the DPPH- and NO-scavenging assays. In the FRAP assay, a

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18

Ren, Jian, and Zhen Yu Wang. "In Vitro Antioxidant Activity of Pigment Extracted from Fruits of Padus racemosa and Padus virginiana." Advanced Materials Research 183-185 (January 2011): 565–69. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.565.

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The total anthocyanin content (TAC) of pigment extracted from fruits of Padus racemosa and Padus virginiana was determined by pH-differential method. TAC in pigment from fruits of Padus racemosa was higher than that in Padus virginiana. The vitro antioxidant activity of the two kinds of pigment was evaluated by different assays, including DPPH• assay, ABTS•+assay, OH• assay and reducing power assay. The results showed that except for reducing power, pigment from fruit of Padus racemosa showed stronger ABTS•+, DPPH• and OH• radical scavenging activity than pigment from fruit of Padus virginiana

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19

Ajaib, Muhammad, Tanveer Hussain, Saleha Farooq, and Mehrban Ashiq. "Analysis of Antimicrobial and Antioxidant Activities of Chenopodium ambrosioides: An Ethnomedicinal Plant." Journal of Chemistry 2016 (2016): 1–11. http://dx.doi.org/10.1155/2016/4827157.

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The antimicrobial and antioxidant potential of Chenopodium ambrosioides L. was explored. Antimicrobial potential was investigated through zone of inhibition and minimum inhibition concentration whereas antioxidant potential of selected plant was evaluated through different techniques, that is, total phenolic contents, total flavonoids content, DPPH assay, ABTS assay, and metal chelating. It is concluded that both parts showed good to satisfactory antimicrobial and antioxidant results. The maximum antibacterial potential is exhibited by bark macerated in petroleum ether against Bacillus subtili

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20

Logeswari, S.* Indumathy R. Malina Barhana R. "Invitro Antioxidant and Wound Healing Activity of Pun Purai Ennai, A Polyherbal Siddha Formulation." International Journal of Pharmaceutical Sciences 3, no. 1 (2025): 1779–86. https://doi.org/10.5281/zenodo.14709778.

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Pun Purai Ennai, a traditional Siddha formulation from Ethiopia used for wound treatment, lacks scientific validation. This study assessed its in vitro antioxidant and wound-healing activities. Sourced from the government Siddha College in Chennai, the study employed the DPPH radical scavenging and Chick Chorioallantoic Membrane (CAM) assays. The DPPH assay gauged antioxidant potential by measuring free radical neutralization. The CAM assay evaluated wound healing through angiogenesis. Results unveiled, In the DPPH assay, Pun Purai Ennai exhibited substantial, concentration-dependent DPPH radi

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21

Al-Mansori, Bubaker, Wessal Hassan El-Ageeli, Seham Hamad Alsagheer, and Faheem A. F. Ben-Khayal. "Antioxidant Activity- Synergistic Effects of Thymol and Carvacrol." Al-Mukhtar Journal of Sciences 35, no. 3 (2020): 185–94. http://dx.doi.org/10.54172/mjsc.v35i3.271.

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This study was conducted to evaluate the antioxidant activity-synergistic effects of Thymol and Carvacrol using Three assays DPPH, FRAP, and TEAC. In the DPPH assay Carvacrol, Thymol and Carvacrol-Thymol mixture had scavenging activity and this activity increasing by increasing concertation from 50 to 1000ppm. Carvacrol showed a strong antioxidant activity than Thymol, and no synergistic effect in their mixture at tested concertation. In FRAP assay, Carvacrol had the highest antioxidant activity as in DPPH assay result. However, the mixture of Carvacrol and Thymol showed a higher reducing powe

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22

Rahim, N. F. A., N. Muhammad, N. Abdullah, B. A. Talip, and K. H. Poh. "The interaction effect and optimal formulation of selected polyherbal extracts towards antioxidant activity." Food Research 4, no. 6 (2020): 2042–48. http://dx.doi.org/10.26656/fr.2017.4(6).281.

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Past study showed that lemongrass (Cymbopogon citratus), curry leaves (Murrya koenigii), turmeric (Curcuma longa) and ginger (Zingiber officinale) contain phytochemicals associated with antioxidant properties. However, all the herbs are tested individually and rarely mix together. This study was conducted to examine the antioxidant properties and interaction effect when combined. The plants studied were decocted with distilled water. Eighteen formulations of aqueous extracts were established using simplex lattice mixture design that was generated by Design Expert software. The antioxidant prop

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P, SHISA ULLAS, NAMITA NAMITA, KANWAR P. SINGH, et al. "Influence of drying techniques on retention of anthocyanin and their antioxidant activities in chrysanthemum (Chrysanthemum x morifolium) flowers." Indian Journal of Agricultural Sciences 88, no. 2 (2018): 228–33. http://dx.doi.org/10.56093/ijas.v88i2.79189.

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The influence of different drying methods, viz. shade drying, hot air oven drying and microwave oven drying on total anthocyanin content, total phenolic content and their antioxidant activities (measured by CUPRAC, FRAP and DPPH assay) of dried ray florets of five anthocyanin rich chrysanthemum (Chrysanthemum × morifolium Ramat.) varieties was studied in the present investigation. Microwave oven drying was the best method w.r.t. retaining its total anthocyanin content, total phenolic content and antioxidant activities. Microwave oven drying method had retained highest total anthocyanins (208.2

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Geng, Rui, Lei Ma, Liangliang Liu, and Yixi Xie. "Influence of Bovine Serum Albumin-Flavonoid Interaction on the Antioxidant Activity of Dietary Flavonoids: New Evidence from Electrochemical Quantification." Molecules 24, no. 1 (2018): 70. http://dx.doi.org/10.3390/molecules24010070.

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Interaction between dietary flavonoids and albumins plays an important role in the bioavailability and bioactivity of flavonoids. Therefore, the influence of this interaction on the antioxidant activity of flavonoid has attracted much interest. In this study, a ceric reducing/antioxidant capacity assay (CRAC) was employed to investigate the effects of albumin-flavonoid interaction on the antioxidant activity of seven common flavonoids. The results obtained from the CRAC assay were also compared separately with the results from the spectrophotometric methods including 2,2-diphenyl-1-picrylhydra

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Fitriana, Wiwit Denny, Taslim Ersam, Kuniyoshi Shimizu, and Sri Fatmawati. "Antioxidant Activity of Moringa oleifera Extracts." Indonesian Journal of Chemistry 16, no. 3 (2018): 297. http://dx.doi.org/10.22146/ijc.21145.

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Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.

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Zhang, Sheng, Xiang Zhou Li, Zhi Ping Wu, and Chun Tao Kuang. "Antioxidant Activity of Polysaccharide from Camellia Cake against ABTS and DPPH Free Radicals." Advanced Materials Research 550-553 (July 2012): 1545–49. http://dx.doi.org/10.4028/www.scientific.net/amr.550-553.1545.

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The ABTS, and DPPH assays were used for determining to investigate the antioxidant effect in vitro of Polysaccharide from Camellia Cake. Different concentrations of the sample were determinated by assay method. Results showed Polysaccharide from Camellia Cake had an appreciable antioxidant effect in a concentration-dependent manner. The EC50 values against DPPH and ABTS free radicals were 0.106 mg/ml and 1.05mg/ml respectively.

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Zhang, Xiu-Li, Yong-Dong Zhang, Tao Wang, Hong-Yun Guo, Qi-Ming Liu, and Hai-Xiang Su. "Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods." Journal of Chemistry 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/249485.

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Several assays have been frequently used to estimate antioxidant capacities includingABTS•+, DPPH, and FRAP assays. Xanthohumol (XN), the major prenylated flavonoid contained in beer, witnessed various reports on its antioxidant capacity. We systematically evaluated the antioxidant activity of XN using three systems, 2,2,-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+) scavenging assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assays, and ferric reducing antioxidant power (FRAP) assays. The results are expressed as Trolox equivalent antioxidant capacity (TEAC). The TEAC of XN wa

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Ng, K. S., Zamzahaila Mohd Zin, Nurmahani Mohd Maidin, and M. K. Zainol. "The ramification of fermentation time on antioxidant properties of Napier grass herbal tea by black tea processing method." Food Research 4, no. 2 (2019): 349–57. http://dx.doi.org/10.26656/fr.2017.4(2).265.

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Tea fermentation relates to the enzymatic oxidation of the leaves of the tea and the formation of dark pigments. This method is usually conducted in a setting where temperature and humidity can be regulated. However, the impact of fermentation time on the antioxidant characteristics of Napier grass herbal tea is still unanswered. This study aimed to determine the effect of fermentation time on antioxidant properties of Napier grass black tea. Napier grass was subjected to fermentation for 3, 6, 9, 12 and 24 hrs. Fresh and dried samples were extracted in water (95°C, 30 mins) and the extracts w

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Yulyana, A., E. Mugiyanto, D. G. Guntara, et al. "Radical scavenging activity of ethanolic extract of green and red leaves of Cantigi (Vaccinium varingiaefolium Bl. Miq.)." Food Research 9, no. 2 (2025): 13–19. https://doi.org/10.26656/fr.2017.9(2).607.

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Indonesia's Cantigi (Vaccinium varingiaefoilum Bl. Miq.) is a member of the blueberry family that grows abundantly around volcanoes. It has two distinct leaf colors, red color leave (RCL) and green color leave (GCL), which contain secondary metabolites including anthocyanins. This study aimed to characterize the antioxidant properties of Cantigi leaf using various methods. DPPH (2,2’-diphenyl-1-picrylhydrazyl), BHA (butylhydroxyanisole), and BHT (butyl hydroxytoluene) were used to check the antioxidant activity of 96% ethanolic Cantigi extract. The phytochemical screening showed positive resul

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Firuzi, Omidreza, Katayoun Javidnia, Maryam Gholami, Mohammad Soltani, and Ramin Miri. "Antioxidant Activity and Total Phenolic Content of 24 Lamiaceae Species Growing in Iran." Natural Product Communications 5, no. 2 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500219.

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The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active

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Song, Ru, Rong Bian Wei, and Hong Yu Luo. "Thermal Treatment to Improve the Antioxidant Activity of Half-Fin Anchovy Pepsin Hydrolysate and Application in Beef Storage." Advanced Materials Research 479-481 (February 2012): 299–304. http://dx.doi.org/10.4028/www.scientific.net/amr.479-481.299.

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In this study, the antioxidant activity and browning intensity of thermal products of half-fin anchovy pepsin hydrolysate (HAHp) were investigated. Thermal treatment, in particular at 121 °C, increased the 1, 1-diphenyl-2-picrylhydrazayl (DPPH) radical scavenging rates. The browning intensity ranging from 0.082 to 0.372 displayed a linear correlation (R2=0.9343) with their corresponding scavenging DPPH radical rates. During 12 days of storage at 4 °C, the antioxidant activity of ground beef meat with addition of heated HAHp (HAHp-H, 121°C for 30 min) was determined using the following assays:

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A. PUSHPARANI, S.VALLI, and S. SHAMINI. "EVALUATION OF PHYTOCHEMICAL AND ANTIOXIDANT PROPERTIES OF VARIOUS HALOPHYTES COLLECTED FROM PICHAVARAM REGION." Asian Journal of Microbiology, Biotechnology & Environmental Sciences 25, no. 02 (2023): 250–55. http://dx.doi.org/10.53550/ajmbes.2023.v25i02.011.

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Mangrove halophytes are highly influential in economical utilities such as food, fuel and Medicinals. Antioxidant and Phytochemical activities were highly significant in these samples. The synthesis of metabolites such as phenols, flavonoids and other bioactive molecules were observed and analysed. In the current script the phytochemical screening and antioxidant assays such as DPPH and ABTS were carried out for aqueous, methanol and hexane extracts of mangrove halophytes such as Ipomoea I. pescaprae, Sesuvium portulacastrum and Suaeda maritima. The preliminary phytochemical screening indicate

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Pathak, Ashutosh, Rahul Das, Deepu Vijayan, Nripemo Odyuo, David L. Baite, and Ashiho A. Mao. "UHPLC analysis and in vitro biological activities of Areca triandra Roxb. ex Buch.-Ham.: a substitute of areca nut." Research Journal of Biotechnology 16, no. 12 (2021): 109–18. http://dx.doi.org/10.25303/1612rjbt109118.

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Areca triandra Roxb. ex Buch.-Ham. (Arecaceae) distributed in Assam, North Bengal plains, Meghalaya andaman and Nicobar Islands is used as a substitute for A. catechu L. (Areca Nut). A. catechu is widely used as masticator in South Asia. The present study was conducted to find out the phenolic acids present in A. triandra and potential of it in inhibiting the reactive oxygen species (ROS). Plant extracts obtained from leaf and nut were analyzed qualitatively, quantitatively and phenolic acids were identified and quantified using reverse phase chromatography method developed on through Hypersil

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Medda, Silvia, Angela Fadda, Leonarda Dessena, and Maurizio Mulas. "Quantification of Total Phenols, Tannins, Anthocyanins Content in Myrtus communis L. and Antioxidant Activity Evaluation in Function of Plant Development Stages and Altitude of Origin Site." Agronomy 11, no. 6 (2021): 1059. http://dx.doi.org/10.3390/agronomy11061059.

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Most of the biological properties of myrtle (Myrtus communis L.) are linked to the antioxidant activity of the phenolic compounds present in the extracts. In this study, the content of total phenols, tannins, and anthocyanins of acidified ethanol extracts of berries and leaves of five myrtle cultivars was quantified during the whole fruit development. In some development stages, the antioxidant activity (AA) of both tissues was evaluated with FRAP, ABTS, DPPH, β-carotene assays, and spectroscopic method (EPR). The trend of AA in leaf and berry extracts was different among genotypes and evaluat

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Kumar A, Ajesh, S. S. Syed Abuthahir, and Hassan Y. Aboul-Enein. "Phytochemical extraction and comparative analysis of antioxidant activities of Areca catechu L. nut extracts." Pharmacia 69, no. 2 (2022): 447–51. http://dx.doi.org/10.3897/pharmacia.69.e77829.

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FRAP assay proved all the extracts of Areca catechu L. nut have antioxidant properties because IC50 values of all the extracts of the same were less than that of ascorbic acid. Remaining antioxidant assays like DPPH radical scavenging assay, H2O2 scavenging assay, and Fe2+ chelating assay showed more antioxidant properties in ethyl acetate extract and nonpolar solvent extracts like n- hexane, and chloroform respectively. Antioxidant properties of Areca catechu L. nut varied depending upon the different solvent extract.

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Kedare, Sagar B., and R. P. Singh. "Genesis and development of DPPH method of antioxidant assay." Journal of Food Science and Technology 48, no. 4 (2011): 412–22. http://dx.doi.org/10.1007/s13197-011-0251-1.

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Uddin, Nizam. "DPPH Scavenging Assay of Eighty Four Bangladeshi Medicinal Plants." IOSR Journal of Pharmacy and Biological Sciences 6, no. 5 (2013): 66–73. http://dx.doi.org/10.9790/3008-0656673.

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Devi, Barri Indira, J. Kalaivannan, Sumathi Jones, et al. "Antioxidant Studies of Ayurvedic Medicine, Dhanwanthararishtam." Journal of Pharmacy and Bioallied Sciences 16, Suppl 5 (2024): S4708—S4711. https://doi.org/10.4103/jpbs.jpbs_875_24.

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ABSTRACT Background: Dhanwanthararishtam is a classical Ayurvedic medicine, primarily used for postnatal care and rejuvenation. Aim: The present study evaluates the antioxidant properties of the Ayurvedic medicine Dhanwanthararishtam, traditionally used for postnatal care to rejuvenate and strengthen the mother. Methods: ABTS, DPPH, and FRAP, were the antibiotic assays carried out following standard protocols. Results: Among these assays, Dhanwanthararishtam exhibited exceptional results at very low concentrations in the FRAP assay, while the ABTS and DPPH assays showed moderate antioxidant ac

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Hegazi, Ahmed G., and Faten K. Abd El-Hady. "Influence of Honey on the Suppression of Human Low Density Lipoprotein (LDL) Peroxidation (In Vitro)." Evidence-Based Complementary and Alternative Medicine 6, no. 1 (2009): 113–21. http://dx.doi.org/10.1093/ecam/nem071.

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The antioxidant activity of four honey samples from different floral sources (Acacia, Coriander, Sider and Palm) were evaluated with three different assays; DPPH free radical scavenging assay, superoxide anion generated in xanthine–xanthine oxidase (XOD) system and low density lipoprotein (LDL) peroxidation assay. The dark Palm and Sider honeys had the highest antioxidant activity in the DPPH assay. But all the honey samples exhibited more or less the same highly significant antioxidant activity within the concentration of 1mg honey/1 ml in XOD system and LDL peroxidation assays. The chemical

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Nguyễn Thị Cúc, Nguyễn Thị Cúc Nguyễn Thị Cúc, Nguyễn Công Thùy Trâm, Đỗ Thị Phương, et al. "The antioxidant and in vitro hepatoprotective activities of some chemical fractions from Phyllanthus reticulates Poir. plant." Vietnam Journal of Biotechnology 15, no. 2 (2018): 251–58. http://dx.doi.org/10.15625/1811-4989/15/2/12341.

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The Phen-den plant (Phyllanthus reticulatus Poir.) has been widely used in Vietnamese tradional medicine for detoxification, antiseptic, diuretic, anti-inflammation treatments…However, there is still lack of studies on this plant’s antioxidant and in vitro hepatoprotective activities in Vietnam. Recently, the use of liver enzymes such as CYP450 reductase (CPR) in determining the in vitro hepatoprotective activities of potential compounds, which are originated from herbal plants, is increasing based on its high efficiency and short duration. Furthermore, the other in vitro assays such as DPPH (

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Sukandar, Dede, Nina Artanti, Ika Restu Purwanti, Tarso Rudiana, and Fitriyanti Fitriyanti. "Antioxidant and Cytotoxicity Activities of the Fermentation Extract of the Endophytic Fungi from the Marine Biota of Colt Coral." ALCHEMY 9, no. 2 (2021): 63–72. http://dx.doi.org/10.18860/al.v9i2.11899.

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Indonesia has the largest and most biodiverse coral reef in the world. Colt coral has not been studied and explored especially endophytic fungi associated with the coral. Endophytic fungi are highly potential for the production of antioxidant and anticancer compounds. This research aimed to study the antioxidant and cytotoxic activities of fermentation extract from endophytic fungi from colt coral. Filtrate and mycelium extracts were obtained from static and shake fermentations of isolate SKF 15. Antioxidant and cytotoxic assays were conducted by free radical scavenger 1,1-diphenyl-2-picrylhyd

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Tuty S, Fidrianny I, and Sukrasno. "Antioxidant profile of chicken bile extract and correlation with chemical content." International Journal of Research in Pharmaceutical Sciences 10, no. 3 (2019): 2311–17. http://dx.doi.org/10.26452/ijrps.v10i3.1470.

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Component in chicken bile may be similar to bear bile, which contains an immunostimulant agent ursodeoxycholic acid and related to antioxidant effect. The purposes of this study were to evaluate the antioxidant potential of chicken bile extract by the DPPH method, analyze the correlation between total phenolic and flavonoid content with IC50 of DPPH scavenging activities. Chicken bile was cut and dried using freeze dryer, then extracted by reflux using different polarity solvents. A rotary evaporator was used to concentrate the extracts. Calculation of total flavonoid and phenolic content, ant

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Platzer, Melanie, Sandra Kiese, Thomas Herfellner, Ute Schweiggert-Weisz, and Peter Eisner. "How Does the Phenol Structure Influence the Results of the Folin-Ciocalteu Assay?" Antioxidants 10, no. 5 (2021): 811. http://dx.doi.org/10.3390/antiox10050811.

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Plants produce a diverse array of secondary metabolites that are generally nonessential but facilitate ecological interactions. Fruits, vegetables, seeds and nuts can accumulate bioactive secondary metabolites with health-promoting properties, including the potent antioxidant activities of phenolic compounds. Several in vitro assays have been developed to measure the polyphenol content and antioxidant activity of plant extracts, e.g., the simple and highly popular Folin-Ciocalteu (FC) assay. However, the literature contains a number of different descriptions of the assay and it is unclear whet

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Ugwo, Jeremiah, and Marvellous Kehinde Adealawode. "Determination of Antioxidant activities of locally produce rice in Northern and Western part of Nigeria, using Kebbi rice and Igbemo rice as case study." Catalyx : Journal of Process Chemistry and Technology 1, no. 1 (2024): 33–44. http://dx.doi.org/10.61978/catalyx.v1i1.238.

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This research provides the antioxidant activities of locally produced rice in the Northern and Western regions of Nigeria, with a focus on Kebbi rice and Igbemo rice. The study utilizes the Ferric Reducing Antioxidant Power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assays to evaluate the antioxidant capacity of these rice varieties. In which the sample was extracted using solvent extraction method after which the antioxidant (AO) activity of the rice samples as ferric-reducing power, is determined using a modified FRAP (ferric reducing/antioxidant power) assay Sodium phosphate, buffer so

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Gang, Yehui, Tae-Yang Eom, Svini Dileepa Marasinghe, Youngdeuk Lee, Eunyoung Jo, and Chulhong Oh. "Optimising the DPPH Assay for Cell-Free Marine Microorganism Supernatants." Marine Drugs 19, no. 5 (2021): 256. http://dx.doi.org/10.3390/md19050256.

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Antioxidants prevent ageing and are usually quantified and screened using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. However, this assay cannot be used for salt-containing samples, such as the cell-free supernatants of marine microorganisms that are aggregated under these conditions. Herein, the DPPH solvent (methanol or ethanol) and its water content were optimized to enable the analysis of salt-containing samples, aggregation was observed for alcohol contents of >70%. The water content of methanol influenced the activities of standard antioxidants but did not significantly affect tha

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Gözcü, Sefa, and Muhammed Atmaca. "Antioxidant Potential and Phenolic Content of Plantago major." Current Research in Health Sciences 2, no. 1 (2025): 27–32. https://doi.org/10.62425/crihs.1628360.

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Objective: This study aimed to evaluate the antioxidant capacity and bioactive compound content of the aerial parts of Plantago major, a medicinal plant with potential therapeutic applications. Methods: The aerial parts were extracted using 70% methanol, and the solvent was evaporated. Total phenolic content (TPC) and total flavonoid content (TFC) were quantified. Antioxidant capacity was assessed using DPPH, ABTS, and FRAP assays. Results: The DPPH radical scavenging assay showed an IC50 value of 127.33 ± 1.07 µg/mL, while the ABTS assay revealed an IC50 value of 46.74 ± 2.53 µg/mL. The FRAP

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Prvulović, Dejan, Đorđe Malenčić, Mirjana Ljubojević, Goran Barać, and Vladislav Ognjanov. "Phenolic Compounds and Antioxidant Capacity of Sweet Cherry Fruits from Vojvodina Province." Contemporary Agriculture 68, no. 1-2 (2019): 1–6. http://dx.doi.org/10.2478/contagri-2019-0001.

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Summary Sweet cherries (Prunus avium L.) contain various phenolic compounds which contribute to total antioxidant activity. The present study was conducted to assess the antioxidant activity of 15 sweet cherry cultivars from Vojvodina province (north Serbia). The free radical scavenging properties of fruits were evaluated employing two different methodologies, including DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. Strong correlations were found between total phenolics, tannins, flavonoids and anthocyanins and DPPH and FRAP as

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M, Kotteswari, Mudiganti Ram Krishna Rao, Prabhu K, Siva Kumar, and Sampad Shil. "ANTIOXIDANT STUDIES OF ONE AYURVEDIC MEDICINE ASWAGANDHARISHTAM." Asian Journal of Pharmaceutical and Clinical Research 11, no. 11 (2018): 227. http://dx.doi.org/10.22159/ajpcr.2018.v11i11.27593.

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Objective: The present study deals with the antioxidant study of one Ayurvedic medicine Aswagandhrishtam by three different methods, namely reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays.Methods: Aswagandharishtam, which is a liquid medicine, was taken as such at various concentrations for all the three assays.Results: The results show that Aswagandharistam has good antioxidant potential when compared with ascorbic acid as standard. The IC50 values of reducing power assay were 250.142, that of DPPH were 103.607, and

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Krishnan, Vijendren, Syahida Ahmad, and Maziah Mahmood. "Antioxidant Potential in Different Parts and Callus ofGynura procumbensand Different Parts ofGynura bicolor." BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/147909.

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Plants from Gynura family was used in this study, namely,Gynura procumbensandGynura bicolor.Gynura procumbensis well known for its various medicinal properties such as antihyperglycaemic, antihyperlipidaemic, and antiulcerogenic; meanwhile,G. bicolorremains unexploited. Several nonenzymatic antioxidants methods were utilized to study the antioxidant capacity, which include ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, total flavonoid content, total phenolic content, and ascorbic acid content determination. DPPH assay revealsG. procumbe

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Emmanuel Mensah, Abigail Donkor, and Mercy Badu. "Investigating the nutritional, antioxidant and antimicrobial properties of Tamarindus indica linn seed and pulp." International Journal of Science and Research Archive 14, no. 1 (2025): 1274–80. https://doi.org/10.30574/ijsra.2025.14.1.0169.

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This study seeks to investigate the nutritional (i.e proximate composition), antioxidant and anti-microbial properties of Tamarindus indica Linn seed and pulp. The proximate composition estimated during the study was analyzed by using standard AOAC methods. Ethanolic, aqueous and ethyl acetate extracts were prepared from seed and pulp powders of Tamarind. The tamarind seed was found to have a substantial protein value (17.51%) which means that it can be processed and used as a food supplement. Phytochemical screening of the samples revealed the presence of flavonoids and tannins in pulp and sa

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