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Journal articles on the topic 'DPPH radical assay'

Author: Grafiati

Published: 3 June 2025

Last updated: 1 August 2025

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1

Gulcin, İlhami, and Saleh H. Alwasel. "DPPH Radical Scavenging Assay." Processes 11, no. 8 (2023): 2248. http://dx.doi.org/10.3390/pr11082248.

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Today, there is an increasing interest in antioxidants, especially to prevent the known harmful effects of free radicals in human metabolism and their deterioration during processing and storage of fatty foods. In both cases, natural-source antioxidants are preferred over synthetic antioxidants. So, there has been a parallel increase in the use of assays to estimate antioxidant efficacy in human metabolism and food systems. Today, there are many bioanalytical methods that measure the antioxidant effect. Of these, the 1,1-diphenyl-2-picrylhydrazil (DPPH) removing assay is the most putative, pop

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2

Darshika, Acharya Meenakshi Vaidya*. "In-vitro antioxidant activity of hydroalcoholic extract of leaves of Hydnocarpus pentandrus (Buch. - Ham) Oken." International Journal of Pharmaceutical Sciences 2, no. 11 (2024): 167–72. https://doi.org/10.5281/zenodo.14030494.

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Cellular damage can arise from the reactions of free radicals with membrane lipids, nucleic acids, proteins, enzymes, and other micro molecules. Free radical-induced cell damage seems to be a primary factor in the aging process and degenerative diseases, including but not limited to cancer, heart disease, cataracts, liver disorders, diabetes mellitus, inflammation, and renal failure. Naturally, the body produces free radicals, and antioxidants scavenge them to shield the body from harmful consequences. This dynamic equilibrium exists between the two. It's possible that there aren't enough anti

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3

Kalaiyarasi, D., V. Manobharathi, and S. Mirunalini. "Capsaicin in hot chili peppers as a potent free radical scavenger: An in vitro approach." Research Journal of Biotechnology 17, no. 4 (2022): 68–75. http://dx.doi.org/10.25303/1704rjbt6875.

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In humans, countless chronic conditions are connected with the accumulation of free radicals. Antioxidants can scavenge free radicals and reduce their effect. The hunt for natural antioxidants of plant provenance becomes an urgent primary concern. So, the present study was to investigate the antioxidant and free radical scavenging activity of Capsaicin by using various in vitro assays such as 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azino-bis(3- ethylbenzthiazoline-6-sulfonic acid) (ABTS●+), hydroxyl radical, superoxide anion, hydrogen peroxide, nitric oxide and reducing power.

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4

Yulyana, A., E. Mugiyanto, D. G. Guntara, et al. "Radical scavenging activity of ethanolic extract of green and red leaves of Cantigi (Vaccinium varingiaefolium Bl. Miq.)." Food Research 9, no. 2 (2025): 13–19. https://doi.org/10.26656/fr.2017.9(2).607.

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Indonesia's Cantigi (Vaccinium varingiaefoilum Bl. Miq.) is a member of the blueberry family that grows abundantly around volcanoes. It has two distinct leaf colors, red color leave (RCL) and green color leave (GCL), which contain secondary metabolites including anthocyanins. This study aimed to characterize the antioxidant properties of Cantigi leaf using various methods. DPPH (2,2’-diphenyl-1-picrylhydrazyl), BHA (butylhydroxyanisole), and BHT (butyl hydroxytoluene) were used to check the antioxidant activity of 96% ethanolic Cantigi extract. The phytochemical screening showed positive resul

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De, A., P. Chattopadhyay, and M. Singh. "In-Vitro Antioxidant Activity and Free Radical Scavenging Potential of Phlorizin Derived Sodium Glucose Cotransporter 2 Inhibitor." Journal of Drug Delivery and Therapeutics 9, no. 4 (2019): 257–64. http://dx.doi.org/10.22270/jddt.v9i4.3038.

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Objectives- In vitro antioxidant activity assay is the preliminary step to determine a drug’s efficacy in combating oxidative stress when used in a clinical condition. The current study was aimed to determine the in vitro antioxidant activity of antidiabetic drug-SGLT-2 inhibitor (canagliflozin, dapagliflozin and empagliflozin) in combating the oxidative stress in diabetes mellitus. Methods- A total of five methods were adopted for determining the antioxidant potential of the drugs. The methods were DPPH radical scavenging assay, nitric oxide radical scavenging assay, phosphomolybdenum assay,

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6

Luo, Aoxue, Ping Peng, Wen Feii, Linying Yang, and Yijun Fan. "Isolation and antioxidant activity (in vitro and in vivo) of the flavonoid from Tartarian-buckwheat." Journal of Scientific and Innovative Research 3, no. 2 (2014): 168–72. http://dx.doi.org/10.31254/jsir.2014.3210.

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The total flavonoid was isolated from tartarian-buckwheat through reflux extraction. In order to explore the antioxidant activities, in the present study, our study aimed to examine the antioxidant activity of the total flavonoid using different assays including: reducing power, 2,2- diphenyl 1-picrylhydrazyl (DPPH) radical scavenging assay, ABTS radical scavenging assay and hydroxyl radical scavenging activity. The results exhibited that the total flavonoid has significant reductive ability and radicals scavenging effects. In vivo antioxidant activities assays, the flavonoid was found to incr

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7

Tumbas, Vesna, Gordana Cetkovic, Sonja Djilas, et al. "Antioxidant activity of mandarin (Citrus reticulata) peel." Acta Periodica Technologica, no. 41 (2010): 195–203. http://dx.doi.org/10.2298/apt1041195t.

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Mandarin peel, a waste product coming from juice production, was extracted by conventional extraction with 70% acetone. Content of flavonoids in mandarin peel extract (MPE) was determined by HPLC. Hesperidin was the most dominant flavonoid. Free radical scavenging activity of MPE on stable DPPH radicals and reactive hydroxyl radicals was also evaluated. EC50 value determined in spectrophotometrical DPPH radical assay was 0.179 mg/ml, while this value in ESR spin trapping hydroxyl radical assay was 0.415 mg/ml. Also, MPE showed protective effects in stabilising sunflower oil during accelerated

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8

Pugalenthi, M., M. Pradheeba, S. Vishnu Kumar, G. Vasukipriyadharshini, S. Swathi, and G. Divya Bharathi. "UNVEILING THE PHYTOCHEMICAL PROFILE, SECONDARY METABOLITE QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF CLEMATIS WIGHTIANA WALL. EX WIGHT and ARN." Journal of Advanced Scientific Research 13, no. 03 (2022): 70–77. http://dx.doi.org/10.55218/jasr.202213312.

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Traditionally, the leaves of Clematis wightiana have been used in the treatment of rheumatism, indigestion, headaches, varicose veins, bone problems, nasal congestion and sinus. The present study was conducted to evaluate the phytochemical profile, quantification of secondary metabolites and free radical scavenging capacity of C. wightiana leaf. The total phenolic, tannin and flavanoid content of C. wightiana leaves were quantified and were found to be higher in the ethyl acetate extract. Subsequently, the extracts were subjected to appraise their antioxidant capacity by availing various in vi

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9

Raja, Boobalan, and Kodukkur Pugalendi. "Evaluation of antioxidant activity of Melothria maderaspatana in vitro." Open Life Sciences 5, no. 2 (2010): 224–30. http://dx.doi.org/10.2478/s11535-010-0005-5.

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AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentra

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10

G, Savitha, Vishnupriya V, and Surapaneni Krishnamohan. "IN VITRO ANTIOXIDANT CAPACITY AND FREE RADICAL SCAVENGING ACTIVITIES OF CARDIOSPERMUM HALICACABUM LINN." Asian Journal of Pharmaceutical and Clinical Research 11, no. 7 (2018): 421. http://dx.doi.org/10.22159/ajpcr.2018.v11i7.25044.

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Objective: Free radicals and oxidants are produced in the body during normal cellular metabolism and exposure to pollutions and ionizing radiations. They exert beneficial and harmful effects to the human body. Harmful effects of these radicals are destroyed by either antioxidants of our body in situ or antioxidants which are supplied through foods. Hence, the aim of this present study is to assess the antioxidant and free radical scavenging capacity of the easily available edible plant Cardiospermum halicacabum Linn. (Mudakathan keerai).Methods: Antioxidant and free radical scavenging activiti

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11

Song, Ru, Rong Bian Wei, and Hong Yu Luo. "Thermal Treatment to Improve the Antioxidant Activity of Half-Fin Anchovy Pepsin Hydrolysate and Application in Beef Storage." Advanced Materials Research 479-481 (February 2012): 299–304. http://dx.doi.org/10.4028/www.scientific.net/amr.479-481.299.

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In this study, the antioxidant activity and browning intensity of thermal products of half-fin anchovy pepsin hydrolysate (HAHp) were investigated. Thermal treatment, in particular at 121 °C, increased the 1, 1-diphenyl-2-picrylhydrazayl (DPPH) radical scavenging rates. The browning intensity ranging from 0.082 to 0.372 displayed a linear correlation (R2=0.9343) with their corresponding scavenging DPPH radical rates. During 12 days of storage at 4 °C, the antioxidant activity of ground beef meat with addition of heated HAHp (HAHp-H, 121°C for 30 min) was determined using the following assays:

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12

A. E., Vijaya Kumar, Vinay M. та Seethalakshami S. "Inhibition of free radical activity by dual PPAR α and PPAR γ agonist using analytical assay methods". International Journal of Basic & Clinical Pharmacology 6, № 7 (2017): 1670. http://dx.doi.org/10.18203/2319-2003.ijbcp20172728.

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Background: Overproduction of free radicals involved in the pathology of a wide variety of clinical disorders. Poor glycaemic control in diabetic people leads to free radical production responsible for diabetic related complications. Antioxidants produces resistance against the oxidative stress by scavenging free radicals may useful in treating diabetic related complications. Saroglitazar is a newer antidiabetic drug act on dual Peroxisome Proliferator Receptor Agonist α (PPAR α) and PPAR γ agonist with protection effect on Diabetes mellitus induced lipid dystrophy. Our study was done to evalu

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13

Platzer, Melanie, Sandra Kiese, Thomas Herfellner, Ute Schweiggert-Weisz, Oliver Miesbauer, and Peter Eisner. "Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays." Molecules 26, no. 5 (2021): 1244. http://dx.doi.org/10.3390/molecules26051244.

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Numerous assays were developed to measure the antioxidant activity, but each has limitations and the results obtained by different methods are not always comparable. Popular examples are the DPPH and ABTS assay. Our aim was to study similarities and differences of these two assay regarding the measured antioxidant potentials of 24 phenolic compounds using the same measurement and evaluation methods. This should allow conclusions to be drawn as to whether one of the assays is more suitable for measuring specific subgroups like phenolic acids, flavonols, flavanones, dihydrochalcones or flavanols

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14

Tahir, Muhammad, Muhammad Shahab Khan, Muhammad Bilal, Ashfaq Ahmad, and Mukhtar Ahmad. "In-Vitro Antioxidant Activites of Novel Synthesized Copper (II) Complexes." International Journal of Current Research and Review 14, no. 13 (2022): 60–64. http://dx.doi.org/10.31782/ijcrr.2022.141310.

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Introduction: The term radical is originally implied, to a portion of a molecule which cannot exist independently in nature. The Development of these radicals is driven by thermal hemolysis, high energy radiation and photolysis. Free radical attacks cause cell damage and homeostatic disruption. Targets of free radicals are all kinds of molecules in the body, such as lipids, nucleic acids and proteins are the major targets. Antioxidants are substances capable of slowing or stopping oxidation pathways that occur under the effect of reactive oxygen moieties or free atmospheric oxygen. Objectives:

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15

Fitriana, Wiwit Denny, Taslim Ersam, Kuniyoshi Shimizu, and Sri Fatmawati. "Antioxidant Activity of Moringa oleifera Extracts." Indonesian Journal of Chemistry 16, no. 3 (2018): 297. http://dx.doi.org/10.22146/ijc.21145.

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Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.

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16

Cheng, Zhihong, Jeffrey Moore, and Liangli (Lucy) Yu. "High-Throughput Relative DPPH Radical Scavenging Capacity Assay." Journal of Agricultural and Food Chemistry 54, no. 20 (2006): 7429–36. http://dx.doi.org/10.1021/jf0611668.

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17

Himabindu K and Vijayalakshmi A. "In-vitro Acetylcholinesterase and antioxidant activity of Ficus dalhousie and Melissa parviflora Benth." International Journal of Research in Pharmaceutical Sciences 11, no. 4 (2021): 8065–70. http://dx.doi.org/10.26452/ijrps.v11i4.4817.

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Ficus dalhousie and Melissa parviflora Benth both plants have been used as Tranquiliser, Relaxants, Nervine tonic and Calming aids throughout the world. The present study was aimed to identify the antioxidant potential of the Ethyl acetate and Hydro alcoholic extract of these plants by in vitro methods. Anti-Alzheimer activity of the plant extract were screened by Acetylcholinesterase (AChE) inhibition and antioxidant by DPPH and Hydrogen oxide. The results of the assays were calculated by the percentage inhibition of these free radicals. In Acetylcholinesterase (AChE) assay inhibitory potenti

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18

Venkatachalapathi, A., Abdul Kaffoor H, and S. Paulsamy. "In vitro antioxidant activity and polyphenol estimation of methanolic fruit extract of Carissa spinarum L." Journal of Ayurvedic and Herbal Medicine 3, no. 3 (2017): 122–26. http://dx.doi.org/10.31254/jahm.2017.3304.

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Antioxidant property of methanolic fruit extract of the medicinal tree species, Carissa spinarum was evaluated by studying the contents of total phenolics, tannins and flavonoids, free radical scavenging activity using 1,1-diphenyl-2- picryl hydrozyl (DPPH), hydroxyl radical scavenging activity, reducing power activity, ABTS•+ assay and metal chelating activity. The results of the study revealed that both the parts studied were found to have potent antioxidant activity against DPPH, hydroxyl and ABTS•+ radicals with the IC50 value of 88.98 for methanolic fruit extract for DPPH radicals and 849

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19

Ding, Li, Xiaomin Zhang, and Jiajia Zhang. "Antioxidant Activity In Vitro Guided Screening and Identification of Flavonoids Antioxidants in the Extract from Tetrastigma hemsleyanum Diels et Gilg." International Journal of Analytical Chemistry 2021 (November 23, 2021): 1–11. http://dx.doi.org/10.1155/2021/7195125.

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This study aimed to investigate the extract with high antioxidant activity of Tetrastigma hemsleyanum Diels et Gilg and identify the antioxidant components in vitro. α, α-Diphenyl-β-picrylhydrazyl (DPPH) radical assay, Trolox equivalent antioxidant capacity (TEAC) assay, ferric reducing antioxidant power (FRAP), and hydroxyl radical scavenging method were used to screen the extract with high antioxidant activity. The antioxidant capacity of the extracts was evaluated by the free radical scavenging ability of DPPH. The ability of extracts to scavenge 2, 2’-azino-bis(3-ethylbenzothiazoline-6-sul

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20

Olaitan, Michael O., Cosmas O. Ujowundu, Chiamaka P. Nzebude, et al. "Organic wastes of Citrus sinensis Peels- a source of eco-friendly and sustainable bioactive compounds for promoting health." Asian Journal of Biochemistry, Genetics and Molecular Biology 16, no. 2 (2024): 21–31. http://dx.doi.org/10.9734/ajbgmb/2024/v16i2358.

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To determine the phytochemicals, radical scavenging and antioxidant potential of orange peel extract. Citrus sinensis were subjected to extraction with ethanol. Gas chromatography (GC) was utilized to determine the phytochemical composition of orange peel extract. Hydrogen peroxide, superoxide, nitric oxide, and hydroxyl radical scavenging assays were conducted to assess radical scavenging potential of the extract. Antioxidant activities of the peel extracts were determined via the 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assa

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21

Risdian, Chandra, Wahyu Widowati, Tjandrawati Mozef, Teresa Liliana Wargasetia, and Khie Khiong. "Free Radical Scavenging Activity of Ethanolic Leaves Extract and Its Different Solvent Fractions of Piper betle L. In Vitro." Indonesian Journal of Cancer Chemoprevention 2, no. 1 (2011): 141. http://dx.doi.org/10.14499/indonesianjcanchemoprev2iss1pp141-145.

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Various diseases including cancer and cardiovascular diseases are induced by free radicals via lipid peroxidation, protein peroxidation, DNA damage, and cellular degeneration in the cells. Piper betle L., commonly called betel, belongs to the Piperaceae family. It originated from South and South East Asia, and has been used as a traditional medicine by people there due to its medicinal properties. In the present study, free radical scavenging activity of ethanolic leaves extract of Piper betle L. together with its different solvent fractions (hexane, ethyl acetate, butanol, and water) were eva

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Logeswari, S.* Indumathy R. Malina Barhana R. "Invitro Antioxidant and Wound Healing Activity of Pun Purai Ennai, A Polyherbal Siddha Formulation." International Journal of Pharmaceutical Sciences 3, no. 1 (2025): 1779–86. https://doi.org/10.5281/zenodo.14709778.

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Pun Purai Ennai, a traditional Siddha formulation from Ethiopia used for wound treatment, lacks scientific validation. This study assessed its in vitro antioxidant and wound-healing activities. Sourced from the government Siddha College in Chennai, the study employed the DPPH radical scavenging and Chick Chorioallantoic Membrane (CAM) assays. The DPPH assay gauged antioxidant potential by measuring free radical neutralization. The CAM assay evaluated wound healing through angiogenesis. Results unveiled, In the DPPH assay, Pun Purai Ennai exhibited substantial, concentration-dependent DPPH radi

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23

Shi, Gaosheng, Hyein Kim, and Sangho Koo. "Oxo-Carotenoids as Efficient Superoxide Radical Scavengers." Antioxidants 11, no. 8 (2022): 1525. http://dx.doi.org/10.3390/antiox11081525.

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Oxo-carotenoids containing conjugated carbonyl groups in their chains were designed to be more efficient superoxide radical scavengers than natural carotenoids, β-carotene and canthaxanthin. A practical chain-extension method for polyene dials (e.g., crocetin dial) was also proposed based on Horner–Wadsworth–Emmons olefination. Double aldol condensation between polyene dials and acetophenones with ring substituents produced oxo-carotenoids with substituted benzene rings. The antioxidant activity of oxo-carotenoids was measured using DPPH (radical) and ABTS (cationic radical) scavenging assays

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Sumedh Sunil Chavan, Pratiksha Prabhakar Shekade, and Arvind Shankarao Dhabe. "Antioxidant activity of a Polyherbal formulation ‘ACHF-01’ by DPPH radical scavenging assay." World Journal of Advanced Research and Reviews 26, no. 3 (2025): 1199–202. https://doi.org/10.30574/wjarr.2025.26.3.2265.

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This study evaluates the antioxidant potential of an herbal formulation through the 2, 2-dipheny l – 1- picrylhydrazyl (DPPH) free radical scavenging assay. Polyherbal formulation ‘ACHF-01’ was tested, with ascorbic acid serving as the positive control. The percentage inhibition of DPPH radicals of sample ‘ACHF-01’ demonstrating the 55.78% activity. These findings highlight the potential of specific herbal combinations as natural antioxidants and suggest the need for further phytochemical analysis and In vivo validation.

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SASIDHARAN, M. "IN VITRO ANALYSIS OF ANTIOXIDANT ACTIVITY IN POLYHERBAL PLANT EXTRACT." YMER Digital 21, no. 05 (2022): 1499–514. http://dx.doi.org/10.37896/ymer21.05/g4.

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Most plants are considered to prevent free radicals associated damages by numerous ways including direct scavenging of free radicals and inhibition of enzymes involved in free radical production. The aim of the present study was to examine the antioxidant activities of the hexane from polyherbal plants of Carica papaya, Punica granatum, Catharanthus roseus and Cympbopogan citratus which is long been known to be a very important source of pharmaceutical. Different concentrations of polyherbal extract was tested for anti-oxidant properties using hydrogen peroxide assay. Similarly, the free radic

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Baskaran Subramani and Baradwaj R.G. "Antibacterial, anti-oxidant and in vitro anticancer analysis of Zingiber officinale (L.) Rosc." JOURNAL OF ADVANCED APPLIED SCIENTIFIC RESEARCH 1, no. 6 (2021): 33–49. http://dx.doi.org/10.46947/joaasr16201635.

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Ginger is considered as an important spice with many clinical potential activities. The present study was undertaken to determine antimicrobial, antioxidant and in vitro anticancer activities of rhizome extract of Zingiber officinale. Antimicrobial activity was tested against Staphylococcus aureus, Bacillus subtilis, Vibrio cholera, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Entero toxigenic Excherichia coli (ETEC) and Salmonella typhi by disc diffusion assay. Antioxidant activity was determined by DPPH free radical scavenging assay and ABTS free radical scavenging assay. The ethanolic e

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Devi, Khoirom Ratipiyari, Paonam Priyobrata Singh, Moirangthem Medhapati Devi, and Gurumayum Jitendra Sharma. "Evaluation of Antioxidant Activities of Alpinia galanga (L.) Willd." Biosciences Biotechnology Research Asia 15, no. 4 (2018): 899–908. http://dx.doi.org/10.13005/bbra/2700.

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Present research was designed to evaluate the free radical scavenging capacities and antioxidant activities of rhizome extracts of Alpinia galanga prepared in different solvent systems (60% aqueous methanol, 60% aqueous ethanol and distilled water) using different in vitro chemical assays. Antioxidant components such as total phenolic content (TPC), total flavonoid content (TFC) and ascorbic acid contents of the ginger species were screened. Antioxidant assays employed included sulphur free radical reactivity assay, ferric ion reducing power assay, DPPH free radical scavenging capacity assay,

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Zuofa Zhang, Jie Jin, and Liangen Shi. "Antioxidant Properties of Ethanolic Extract from Ramulus mori (Sangzhi)." Food Science and Technology International 15, no. 5 (2009): 435–44. http://dx.doi.org/10.1177/1082013209350277.

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The antioxidant properties and total phenolic contents of four fractions of ethanolic extract from Ramulus mori were examined. Various experimental models including superoxide radical, hydroxyl radical, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) scavenging activity, metal chelating activity, and reducing power were used for characterization of their antioxidant activity. The four fractions showed various degrees of efficacy in each assay in a dose-dependent manner. The third fraction with the highest amount of total phenolics was the most potent antioxidant in all assays used. In addition,

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Zhang, Xiu-Li, Yong-Dong Zhang, Tao Wang, Hong-Yun Guo, Qi-Ming Liu, and Hai-Xiang Su. "Evaluation on Antioxidant Effect of Xanthohumol by Different Antioxidant Capacity Analytical Methods." Journal of Chemistry 2014 (2014): 1–6. http://dx.doi.org/10.1155/2014/249485.

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Several assays have been frequently used to estimate antioxidant capacities includingABTS•+, DPPH, and FRAP assays. Xanthohumol (XN), the major prenylated flavonoid contained in beer, witnessed various reports on its antioxidant capacity. We systematically evaluated the antioxidant activity of XN using three systems, 2,2,-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS•+) scavenging assays, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assays, and ferric reducing antioxidant power (FRAP) assays. The results are expressed as Trolox equivalent antioxidant capacity (TEAC). The TEAC of XN wa

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A. PUSHPARANI, S.VALLI, and S. SHAMINI. "EVALUATION OF PHYTOCHEMICAL AND ANTIOXIDANT PROPERTIES OF VARIOUS HALOPHYTES COLLECTED FROM PICHAVARAM REGION." Asian Journal of Microbiology, Biotechnology & Environmental Sciences 25, no. 02 (2023): 250–55. http://dx.doi.org/10.53550/ajmbes.2023.v25i02.011.

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Mangrove halophytes are highly influential in economical utilities such as food, fuel and Medicinals. Antioxidant and Phytochemical activities were highly significant in these samples. The synthesis of metabolites such as phenols, flavonoids and other bioactive molecules were observed and analysed. In the current script the phytochemical screening and antioxidant assays such as DPPH and ABTS were carried out for aqueous, methanol and hexane extracts of mangrove halophytes such as Ipomoea I. pescaprae, Sesuvium portulacastrum and Suaeda maritima. The preliminary phytochemical screening indicate

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Ushie, O. A., F. E. Abeng, E. Ogah, V. E. Okpashi, S.O Ama, and N. N. Austin. "Antioxidant Activities of Hexane, Ethyl acetate, Acetone and Methanol, Extracts of Mucuna pruriens." Chemistry Research Journal 6, no. 5 (2021): 47–51. https://doi.org/10.5281/zenodo.11762749.

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<strong>Abstract </strong>Antioxidant activities of methanol, hexane, ethyl-acetate and acetone extracts of <em>Mucuna pruriens</em> leaves were evaluated using 1, 1-diphenyl-2-picrylhdrazyl (DPPH) radical scavenging assay in which all the leaf extract shows remarkable activities. The DPPH radical scavenging assay shows that extracts of methanol and acetone showed a good scavenging activity among all the extracts.

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Shekade, Pratiksha Prabhakar, Sumedh Sunil Chavan, and Arvind Shankarrao Dhabe. "Antioxidant Potential of Arogyavardhini Vati Extract by DPPH Assay." Journal of Drug Delivery and Therapeutics 15, no. 7 (2025): 25–28. https://doi.org/10.22270/jddt.v15i7.7254.

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Arogyavardhini Vati, an Ayurvedic medicine, was commonly used during the COVID-19 pandemic for its immunomodulatory and therapeutic properties. The DPPH radical scavenging assay in this study revealed its antioxidant potential. This Ayurvedic medicine demonstrated considerable antioxidant capability with 78.04% DPPH scavenging activity. These findings support its traditional use and suggest the need for further investigation. Keywords: Arogyavardhini Vati, DPPH assay, antioxidant activity, ethanol extract, COVID-19, Soxhlet’s extraction, etc.

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Brodowska, Katarzyna Małgorzata, Agnieszka Joanna Brodowska, Krzysztof Śmigielski, and Elżbieta Łodyga-Chruścińska. "Antioxidant profile of essential oils and extracts of cinnamon bark (Cinnamomum cassia)." European Journal of Biological Research 6, no. 4 (2016): 310–16. https://doi.org/10.5281/zenodo.197200.

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Nowadays, more and more interest is focused on compounds that inhibit reactions promoted by free radicals. The study shows the antioxidant activity of essential oils isolated from <em>Cinnamomum cassia</em>. The methanolic extracts were also obtained and its scavenging ability investigated. The IC<sub>50</sub> value, defined as the concentration of the sample required for 50% scavenging of DPPH free radicals, was also estimated. DPPH and ABTS assay indicated that lower IC<sub>50</sub> value for cinnamon extracts corresponds to its higher radical scavenging activity (IC<sub>50</sub> = 42.03 µg/

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Manimaran, Manickavelu, and Krishnan Kannabiran. "Marine Streptomyces Sp. VITMK1 Derived Pyrrolo [1, 2-A] Pyrazine-1, 4-Dione, Hexahydro-3-(2-Methylpropyl) and Its Free Radical Scavenging Activity." Open Bioactive Compounds Journal 5, no. 1 (2017): 23–30. http://dx.doi.org/10.2174/1874847301705010023.

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Background: Free radical generation has been proved to be responsible for various cellular diseases. It is necessary to combat free radicals using antioxidants derived from natural sources. Objective: The objective of this study is to evaluate the antioxidant activity of the diketopiperazine compound extracted from Streptomyces sp. VITMK1 isolated from mangrove sediment soil collected from Pichavaram, Tamil Nadu, India. Methods: The antioxidant potential of pyrrolo [1, 2-A] pyrazine-1, 4-dione, hexahydro-3-(2-methylpropyl) (diketopiperazine) extracted from Streptomyces sp. VITMK1 was studied u

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35

Prvulović, Dejan, Đorđe Malenčić, Mirjana Ljubojević, Goran Barać, and Vladislav Ognjanov. "Phenolic Compounds and Antioxidant Capacity of Sweet Cherry Fruits from Vojvodina Province." Contemporary Agriculture 68, no. 1-2 (2019): 1–6. http://dx.doi.org/10.2478/contagri-2019-0001.

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Summary Sweet cherries (Prunus avium L.) contain various phenolic compounds which contribute to total antioxidant activity. The present study was conducted to assess the antioxidant activity of 15 sweet cherry cultivars from Vojvodina province (north Serbia). The free radical scavenging properties of fruits were evaluated employing two different methodologies, including DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. Strong correlations were found between total phenolics, tannins, flavonoids and anthocyanins and DPPH and FRAP as

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36

Coulombier, Noémie, Elodie Nicolau, Loïc Le Déan, Cyril Antheaume, Thierry Jauffrais, and Nicolas Lebouvier. "Impact of Light Intensity on Antioxidant Activity of Tropical Microalgae." Marine Drugs 18, no. 2 (2020): 122. http://dx.doi.org/10.3390/md18020122.

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Twelve microalgae species isolated in tropical lagoons of New Caledonia were screened as a new source of antioxidants. Microalgae were cultivated at two light intensities to investigate their influence on antioxidant capacity. To assess antioxidant property of microalgae extracts, four assays with different modes of action were used: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-éthylbenzothiazoline-6-sulphonique) (ABTS), oxygen radical absorbance capacity (ORAC), and thiobabituric acid reactive substances (TBARS). This screening was coupled to pigment analysis to link antioxidant ac

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Elkamhawy, Ahmed, Na Kyoung Oh, Noha A. Gouda, et al. "Novel Hybrid Indole-Based Caffeic Acid Amide Derivatives as Potent Free Radical Scavenging Agents: Rational Design, Synthesis, Spectroscopic Characterization, In Silico and In Vitro Investigations." Metabolites 13, no. 2 (2023): 141. http://dx.doi.org/10.3390/metabo13020141.

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Antioxidant small molecules can prevent or delay the oxidative damage caused by free radicals. Herein, a structure-based hybridization of two natural antioxidants (caffeic acid and melatonin) afforded a novel hybrid series of indole-based amide analogues which was synthesized with potential antioxidant properties. A multiple-step scheme of in vitro radical scavenging assays was carried out to evaluate the antioxidant activity of the synthesized compounds. The results of the DPPH assay demonstrated that the indole-based caffeic acid amides are more active free radical scavenging agents than the

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Alphonse, Dr Arockia Jenecis, M. Shantha, S. Rega, and Arockia Badhsheeba. "In Vitro Antioxidant Activity Of Red Seaweeds Of Methanolic Extracts From Rameshwaram Coast." International Journal of Environmental Sciences 11, no. 8s (2025): 759–66. https://doi.org/10.64252/nad3q962.

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Natural extracts of marine macroalgae are widely recognized for their antioxidant.The aim of this study was to evaluate the antioxidant activity of methanolic extracts of two marine algae, Haloplegma duperreyi and Halymenia floresii. The extracts were assayed, DPPH free radical scavenging capacity, Hydroxyl and ABT+ radical scavenging assay in both algae. Methanolic extracts of both algae were found to exhibit significant antioxidant activity. Among two seaweeds, Haloplegma duperreyi exhibited a good capacity for DPPH free radical scavenging capacity, Hydroxyl and ABT+ radical scavenging assay

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Ronowicz, Joanna, Bogumiła Kupcewicz, and Elżbieta Budzisz. "Implementation of chemometric techniques for evaluation of antioxidant properties of Camellia sinensis extracts." Open Chemistry 12, no. 6 (2014): 700–710. http://dx.doi.org/10.2478/s11532-014-0530-6.

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AbstractIn this study, antioxidant properties of commercial green teas and dietary supplements containing Camellia sinensis extracts were evaluated. Extracts were examined using two antioxidant assays (DPPH· radical method and ABTS·+ cation radical method). A Folin-Ciocalteu assay was used to evaluate the total polyphenol content in the extracts. In order to compare and characterize the investigated Camellia sinensis extracts, chemometric techniques based on fingerprint chromatograms, antioxidant activity and total polyphenol content were applied. Application of chemometric methods allowed for

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40

Bordt, Evan A., Naibo Zhang, Jaylyn Waddell, and Brian M. Polster. "The Non-Specific Drp1 Inhibitor Mdivi-1 Has Modest Biochemical Antioxidant Activity." Antioxidants 11, no. 3 (2022): 450. http://dx.doi.org/10.3390/antiox11030450.

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Mitochondrial division inhibitor-1 (mdivi-1), a non-specific inhibitor of Drp1-dependent mitochondrial fission, is neuroprotective in numerous preclinical disease models. These include rodent models of Alzheimer’s disease and ischemic or traumatic brain injury. Among its Drp1-independent actions, the compound was found to suppress mitochondrial Complex I-dependent respiration but with less resultant mitochondrial reactive oxygen species (ROS) emission compared with the classical Complex I inhibitor rotenone. We employed two different methods of quantifying Trolox-equivalent antioxidant capacit

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Pascoal, Aislan C. R. F., Carlos Augusto Ehrenfried, Marcos N. Eberlin, Maria Élida Alves Stefanello, and Marcos José Salvador. "Free Radical Scavenging Activity, Determination of Phenolic Compounds and HPLC-DAD/ESIMS Profile of Campomanesia Adamantium Leaves." Natural Product Communications 6, no. 7 (2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600711.

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Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activity to scavenge free radicals. Campomanesia adamantium (Myrtaceae) is a small tree with edible fruit, commonly known as “guavira” or “guabiroba-branca” that has been used in popular medicine as depurative anti-diarrhoeic, anti-inflammatory, anti-rheumatic and to liver diseases. In this study, the antiradical activities of ethanol crude extract of the leaves from C. adamantium and the ethyl acetate and butanol fracti

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Al-Zubaidi, Yassir Mohammed Ali, Arifah Abdul Kadir, and Zainul Amiruddin Zakaria. "The Free Radical Scavenging and Cytotoxic Properties of Ardisia crenata and Peliosanthes teta in the 4T1 Breast Cancer Cell Line." Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ) 7, no. 2 (2024): 101–8. http://dx.doi.org/10.54133/ajms.v7i2.1373.

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Background: Ardisia crenata (A. crenata) and Peliosanthes teta Andrew (P. teta) are popular medicinal plants in Malaysia that are claimed to have several biological effects. Objective: To assess the anticancer and radical-scavenging properties of A. crenata and P. teta methanolic extracts. Methods: TPC was quantified using the Folin-Ciocalteu test; free radical scavenging was evaluated using the 2,2'-azino-bis3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays; and cytotoxic activity was assessed using the MTT assay. Results: In both ABTS and DPPH syst

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Sona, P., and G. Subramanian. "Antioxidant Activities of Marine Green Algae 4 Species of a Genus Enteromorpha from Mandapam Coast, South India." Journal of Plant Science Research 38, no. 2 (2023): 475–84. http://dx.doi.org/10.32381/jpsr.2022.38.02.4.

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The present study focused on determining the antioxidant properties of the Enteromorpha compressa (Linnaeus) Nees, Enteromorpha flexuosa (Wulfen) J. Agardh, Enteromorpha intestinalis (Linnaeus) Nees, and Enteromorpha prolifera (Müller) J. Agardh, ethanol crude extracts, from the green algae. The evaluation of antioxidant properties was estimated and determined by using ten standard methods namely 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS), metal chelating activity, hydrogen peroxide radical assay, superoxide radical scavenging (SOD), redu

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Macáková, Kateřina, Lubomir Opletal, Miroslav Polášek, Věra Samková, and Luděk Jahodář. "Free-radical Scavenging Activity of Some European Boletales." Natural Product Communications 4, no. 2 (2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400219.

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The ethanol extracts of 47 mushroom species from the order Boletales were tested for their free radical scavenging activities by means of the DPPH free-radical scavenging and hydrogen peroxide-induced luminol chemiluminescence assays. In the DPPH radical scavenging test, 26 species gave EC50 values of less than 1 mg/mL. The highest activity was exhibited by Gyrodon lividus (0.05 mg/mL). However, in the hydrogen peroxide-induced luminol chemiluminescence assay, only 4 species had EC50 values of less than 1 mg/mL, with the highest activity being exhibited by Strobilomyces floccopus (0.26 mg/mL).

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Macáková, Kateřina, Lubomír Opletal, Miroslav Polášek, and Věra Samková. "Free-radical Scavenging Activity of some European Polyporales." Natural Product Communications 5, no. 6 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500623.

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The ethanol extracts of 30 mushroom species from the order Polyporales were tested for their free radical scavenging activities by means of the DPPH free-radical scavenging and hydrogen peroxide-induced luminol chemiluminescence assays. In the DPPH radical scavenging test, 7 species gave EC50 values of less than 1 mg/mL. The highest activity was exhibited by Fomitopsis pinicola (0.09 mg/mL). However, in the hydrogen peroxide-induced luminol chemiluminescence assay, only 4 species had EC50 values of less than 1 mg/mL, with the highest activity being exhibited also by F. pinicola (0.26 mg/mL). W

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Messai, Mohamed Ahmed, Mokhtar Saidi, and Hocine Dandougui. "Contribution to the valorization of Herniaria fontanesii J. Gay (Caryophyllaceae) grown in Septentrional Sahara and its antioxidant activity." Research Journal of Chemistry and Environment 27`, no. 9 (2023): 53–59. http://dx.doi.org/10.25303/2709rjce053059.

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This study aims to study a wild plant Herniaria fontanesii J. Gay which grows in Septentrional Sahara in El Oued Algeria. The evaluation of total phenolic and flavonoids content has been estimated in both aqueous and butanolic fractions of 80% methanolic extracts obtained from the plant's aerial part. Antioxidant activity was evaluated in vitro using scavenging assays of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, ferric reducing power FRAP and molybdenum reducing power methods. The extracts showed that the plant ha

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Kuang, Wei, Chen Zhang, Man Rong Huang, and Zhi Wei Liu. "Antioxidant Activities of the 95% Ethanol Extracts of Nine Chinese Herbs Commonly Used in Hakka Area." Advanced Materials Research 396-398 (November 2011): 246–49. http://dx.doi.org/10.4028/www.scientific.net/amr.396-398.246.

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Nine Chinese herbs including Artemisiae Argyi, Peristrophe baphica, Paederia scandense, Patrinia villosa, Cassia mimosoides, Gynostemma Pentaphyllum, Rabdosin serra, Mesona chinensis and Houttuynia cordata are commonly used in Hakka area. We investigated the antioxidant activities of 95% ethanol extracts from whole plants of the nine Chinese herbs by two in vitro assays, namely DPPH radical-scavenging activity and ferric reducing/antioxidant power(FRAP) assay. The contents of total flavonoids and total phenols were measured by aluminum chloride and Folin-Ciocalteu colorimetric assays, respecti

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Alam, Mohammad Khairul, Ziaul Hasan Rana, Sheikh Nazrul Islam, and Mohammad Akhtaruzzaman. "Total phenolic content and antioxidant activity of methanolic extract of selected wild leafy vegetables grown in Bangladesh: A cheapest source of antioxidants." Potravinarstvo Slovak Journal of Food Sciences 13, no. 1 (2019): 287–93. http://dx.doi.org/10.5219/1107.

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Nowadays, more attention has been paid on wild plants as new source of natural antioxidants. Therefore, methanolic extracts of 10 traditionally consumed wild leafy vegetables of Bangladesh were analyzed for their total phenolic content (TPC) and free radical scavenging activity. Folin–Ciocalteu method followed by spectrophotometric measurement was used to quantify the TPC of the selected wild leafy vegetables. Free radical scavenging activity was examined utilizing 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay. Different concentrations of the plant extract were applied to ascertain the dose resp

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Rehman, Gauhar, Muhammad Hamayun, Amjad Iqbal, et al. "In Vitro Antidiabetic Effects and Antioxidant Potential ofCassia nemophilaPods." BioMed Research International 2018 (2018): 1–6. http://dx.doi.org/10.1155/2018/1824790.

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The antidiabetic and antioxidant potential of ethanolic extract ofCassia nemophilapod (EECNP) was evaluated by three in vitro assays, including yeast glucose uptake assay, glucose adsorption assay, and DPPH radical scavenging activity. The result revealed that the extracts have enhanced the uptake of glucose through the plasma membrane of yeast cells. A linear increase in glucose uptake by yeast cells was noticed with gradual increase in the concentration of the test samples. Moreover, the adsorption capacity of the EECNP was directly proportional to the molar concentration of glucose. Also, t

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Kowalczyk, Adam, Izabela Biskup, and Izabela Fecka. "Total Phenolic Content and Antioxidative Properties of Commercial Tinctures Obtained from Some Lamiaceae Plants." Natural Product Communications 7, no. 12 (2012): 1934578X1200701. http://dx.doi.org/10.1177/1934578x1200701223.

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The antioxidant level of commercial tinctures from three Lamiaceae plants, Salvia officinalis, Mentha piperita, and Melissa officinalis, have been determined by the Folin-Ciocalteu method, the 2.2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging technique and ABTS assay. Total phenolic content was expressed as GAE (gallic acid equivalent) and ranged from 0.24 to 3.99 mg/mL. Antioxidant activity in the ABTS assay, calculated as TEAC (Trolox equivalent antioxidant capacity), ranged from 23.5 to 35.6 μmol Trolox/mL, while in the DPPH method, the EC50 value ranged from 0.04 to 0.07 mL/a

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