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Journal articles on the topic 'DPPH Scavenging Activity'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Liu, Ping, Yun Tao Gao, Jiao Jiao Yu, et al. "DPPH Radical Scavenging Activity of Kaempferol." Advanced Materials Research 781-784 (September 2013): 1294–97. http://dx.doi.org/10.4028/www.scientific.net/amr.781-784.1294.

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The UV-Vis absorption spectrum of kaempferol and DPPH was investigated, the optimum determination wavelength and reaction time for determining the DPPH radical scavenging activity of kaempferol was 517 nm and 30 min, respectively. Kaempferol exhibited strong DPPH radical scavenging activity with a IC50 value of 0.004349 mg·mL-1, which is smaller than that of rutin, indicating that kaempferol has a stronger antioxidant activity than rutin.

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2

K.Ramanji, Reddy* Dr.Chandaka Madhu Dr.J.Balaji Chandra Mouli Dr.B.Jagan Mohan Reddy Penumala Joshi Ballavarapu Sharanya. "COMPARATIVE STUDY ON PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY ON ETHANOLIC EXTRACT OF MUSA ACUMINATE AND MANGIFERA INDICA." Indo American Journal of Pharmaceutical Sciences 04, no. 09 (2017): 2817–29. https://doi.org/10.5281/zenodo.888116.

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The present study was undertaken to investigate in vitro antioxidant activity of alcoholic extract of musa acuminata and mangifera indica . Method and methodlogy: The total Phenolic content was determined using folinciocalteau method while the total flavanoid content was determined using aluminum chloride method. In vitro antioxidant activity was evaluated using the Reducing power assay , Hydrogen peroxide scavenging assay, nitric oxide scavenging activity, and DPPH scavenging activity. Result: In the present study we have conclude that Mangifera Indica has a significant activity than Musa Acu

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3

Dr.ChandakaMadhu, Gunugurthi Ramya Sai Shreeja Alikette Deeti Prasanna Adiraju Usha Lalitha Gollapalli Shivapoojitha Meher Fathima. "COMPARATIVE STUDY ON PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY ON ETHANOLIC EXTRACT OF ACTINIDIA DELICIOSA AND VITIS VINFERA." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 01 (2018): 735–44. https://doi.org/10.5281/zenodo.1174193.

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The present study was undertaken to investigate in vitro antioxidant activity of alcoholic extract of Actinidia Deliciosa And Vitis Vinfera. Method and methodlogy: The total Phenolic content was determined using folinciocalteau method while the total flavanoid content was determined using aluminum chloride method. In vitro antioxidant activity was evaluated using the Reducing power assay , Hydrogen peroxide scavenging assay, nitric oxide scavenging activity, and DPPH scavenging activity. Result: In the present study we have conclude that Vitis Vinfera, has a significant activity than Actinidia

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4

Gulcin, İlhami, and Saleh H. Alwasel. "DPPH Radical Scavenging Assay." Processes 11, no. 8 (2023): 2248. http://dx.doi.org/10.3390/pr11082248.

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Today, there is an increasing interest in antioxidants, especially to prevent the known harmful effects of free radicals in human metabolism and their deterioration during processing and storage of fatty foods. In both cases, natural-source antioxidants are preferred over synthetic antioxidants. So, there has been a parallel increase in the use of assays to estimate antioxidant efficacy in human metabolism and food systems. Today, there are many bioanalytical methods that measure the antioxidant effect. Of these, the 1,1-diphenyl-2-picrylhydrazil (DPPH) removing assay is the most putative, pop

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5

Alisi, Maria Alessandra, Mario Brufani, Nicola Cazzolla, et al. "DPPH radical scavenging activity of paracetamol analogues." Tetrahedron 68, no. 49 (2012): 10180–87. http://dx.doi.org/10.1016/j.tet.2012.09.098.

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6

Siriwardhana, Nalin, K. W. Lee, Y. J. Jeon, S. H. Kim, and J. W. Haw. "Antioxidant Activity of Hizikia fusiformis on Reactive Oxygen Species Scavenging and Lipid Peroxidation Inhibition." Food Science and Technology International 9, no. 5 (2003): 339–46. http://dx.doi.org/10.1177/1082013203039014.

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Water and organic extracts (diethyl ether, chloroform, ethyl acetate, acetone, ethanol and methanol) obtained from Hizikia fusiformis were screened on reactive oxygen species (ROS) scavenging assays (1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydrogen peroxide and hydroxyl radical) and lipid peroxidation (inhibition of linoleic acid oxidation) inhibitory assays. Water, methanol and ethanol extracts showed significant ROS radical scavenging activities. Water extracts showed high scavenging activities on hydrogen peroxide (around 76%) and DPPH radicals (around 75%) while it presente

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7

She, Gai-Mei, Jin-Bo Hu, Ying-Jun Zhang, and Chong-Ren Yang. "Phenolic constituents fromRhopalocnemis phalloideswith DPPH radical scavenging activity." Pharmaceutical Biology 48, no. 1 (2009): 116–19. http://dx.doi.org/10.3109/13880200903032757.

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8

Ouaket, Amine, Fatiha Moughaoui, Asmae Laaraibi, Souad Hamdouch, Mohammed Berrada, and Noureddine Knouzi. "DPPH scavenging activity of some Bis-benzimidazole derivatives." Mediterranean Journal of Chemistry 8, no. 2 (2019): 103. http://dx.doi.org/10.13171/10.13171/mjc8219041101ao.

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<p>As part of our research on substituted benzimidazoles, we are interested in the synthesis of new heterocyclic molecules. This new organic molecule is a subclass of quinolines with a wide variety of biological properties. In order to affect the binding of quinoline to our bis-benzimidazole derivatives, we have chosen the "azo" bond as a means of attachment. To achieve our goal, we investigated different parameters for the reactions to determine the conditions to obtain the best results. This article discusses the antioxidant activity of our molecules using the DPPH method.</p>

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9

Ouaket, Amine, Fatiha Moughaoui, Asmae Laaraibi, Souad Hamdouch, Mohammed Berrada, and Noureddine Knouzi. "DPPH scavenging activity of some Bis-benzimidazole derivatives." Mediterranean Journal of Chemistry 8, no. 2 (2019): 103–7. http://dx.doi.org/10.13171/mjc8219041101ao.

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As part of our research on substituted benzimidazoles, we are interested in the synthesis of new heterocyclic molecules. This new organic molecule is a subclass of quinolines with a wide variety of biological properties. In order to affect the binding of quinoline to our bis-benzimidazole derivatives, we have chosen the "azo" bond as a means of attachment. To achieve our goal, we investigated different parameters for the reactions to determine the conditions to obtain the best results. This article discusses the antioxidant activity of our molecules using the DPPH method.

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10

She, Gai-Mei, Ying-Jun Zhang, and Chong-Ren Yang. "Phenolic Constituents fromBalanophora laxiflorawith DPPH Radical-Scavenging Activity." Chemistry & Biodiversity 6, no. 6 (2009): 875–80. http://dx.doi.org/10.1002/cbdv.200800139.

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11

Ambujakshi, H. R. Seru ganapaty B. Ganga Rao. "ANTIOAXIDANT ACTIVITY OF METHANOL EXTRACT OF CUSCUTA HYALINA ROTH." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 06 (2018): 5060–64. https://doi.org/10.5281/zenodo.1286335.

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The aim of the present study was designed to evaluate the in vitro antioxidant activity of the methanol extract of whole plant of Cusuta hyalina Roth. The activity was evaluated by using Diphenyl picryl hydrazyl (DPPH), superoxide and hydroxyl radical scavenging methods. The radical scavenging activity of the extract was found to be concentration dependant and maximum scavenging activity was at a dose of 300 µg and comparable with ascorbic acid. Antioxidant potential could be due to the presence of flavonoids, alkaloids, triterpenoids, glycosides, steroids and carbohydrates. Key words: C

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12

Zhang, Hong, Gui Gan Fang, Hua Zheng, Yuan Heng Guo, and Kun Li. "Study on the Antioxidation of Lac Dye." Applied Mechanics and Materials 140 (November 2011): 451–58. http://dx.doi.org/10.4028/www.scientific.net/amm.140.451.

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To investigate the in vitro antioxidation and antimicrobial activity of the lac dye, in this study, three free radicals DPPH·, ABTS+and O2ˉ· are used as the test objects to evaluate the free radial scavenging ability of the lac dye by comparing the absorbance change of free radical solution under specific wavelength. The results showed that, the lac dye has scavenging activity on DPPH· and ABTS+, but has no scavenging activity on O2ˉ·; when the lac dye reached a certain concentration, its scavenging rate on DPPH· and ABTS+can be as high as 68% and 87% respectively, with the antioxidant activit

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13

Shekade, Pratiksha Prabhakar, Sumedh Sunil Chavan, and Arvind Shankarrao Dhabe. "Antioxidant Potential of Arogyavardhini Vati Extract by DPPH Assay." Journal of Drug Delivery and Therapeutics 15, no. 7 (2025): 25–28. https://doi.org/10.22270/jddt.v15i7.7254.

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Arogyavardhini Vati, an Ayurvedic medicine, was commonly used during the COVID-19 pandemic for its immunomodulatory and therapeutic properties. The DPPH radical scavenging assay in this study revealed its antioxidant potential. This Ayurvedic medicine demonstrated considerable antioxidant capability with 78.04% DPPH scavenging activity. These findings support its traditional use and suggest the need for further investigation. Keywords: Arogyavardhini Vati, DPPH assay, antioxidant activity, ethanol extract, COVID-19, Soxhlet’s extraction, etc.

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14

Kim, Hyeon-Min, Won Hur, and Shin-Young Lee. "Optimization of Hot Water Extraction for Enhancing Antioxidative Activity from Auricularia auricula Using Taguchi Approach." Food Engineering Progress 15, no. 3 (2011): 221–29. http://dx.doi.org/10.13050/foodengprog.2011.15.3.221.

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Optimization study of the hot water extraction for enhancing antioxidative activity from Auricularia auricula was performed by Taguchi approach using orthogonal matrix L9(34) method. The correlation between DPPH radical scavenging activity and the components of samples extracted from different extraction conditions were also analyzed. The correlation coefficient between DPPH radical scavenging activity and melanin content of A. auricula was 0.93, indicating 'good correlation'. The optimum extraction conditions were obtained at the extraction time of 1 hr. temperature of 85°C, solid: water rati

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15

Park, Chan Hum, Takashi Tanaka, Hyun Young Kim, Jong Cheol Park, and Takako Yokozawa. "Protective Effects of Corni Fructus against Advanced Glycation Endproducts and Radical Scavenging." Evidence-Based Complementary and Alternative Medicine 2012 (2012): 1–7. http://dx.doi.org/10.1155/2012/418953.

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We investigated the inhibition of advanced glycation endproduct (AGE) activity using the fluorescence characteristics of fractions and compounds from Corni Fructus. Corni Fructus extract and its iridoid glycoside components showed low inhibitory activities as well as the AGE inhibitor aminoguanidine. However, a low molecular weight polyphenol, 7-O-galloyl-D-sedoheptulose, and an antioxidant, trolox, showed high inhibitory activities compared with aminoguanidine under reactive conditions. The AGE-inhibiting activity of polyphenolic fractions of Corni Fructus ranged from a level comparable to Co

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16

Kumar, Mishra Arvind, Kumar S. Pawan, and Gupta Satish Chand. "ANTIBACTERIAL, ANTIFUNGAL AND DPPH RADICAL SCAVENGING ACTIVITY OF ACHYRANTHES ASPERA L." International Journal of Research in Ayurveda and Pharmacy 13, no. 2 (2022): 17–20. http://dx.doi.org/10.7897/2277-4343.130228.

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The plant Apamarg (Achyranthes aspera L.) was tested for antibacterial (Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus), antifungal (Aspergillus niger and Fusarium oxysporum) activities. For extraction, methanol extract was chosen. The DPPH Radical Scavenging activity was used to test the antioxidant action. The current study reveals a link between indigenous Achyranthes aspera L. treatment and in vitro antibacterial and antifungal responses. Ascorbic acid had the lowest DPPH scavenging activity than methanolic extracts of Achyranthes

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17

Shihab K. K., Mohammed, Rajagopal P. L., and Nasila K. "Antioxidant Screening on the Whole Plant of Cyanthillium cinereum (L) H.Rob." International Journal of Research and Review 8, no. 7 (2021): 458–61. http://dx.doi.org/10.52403/ijrr.20210764.

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Methanolic extract of the whole plant of Cyanthillium cinereum (L) H.Robwasevaluated for free radical scavenging activity by using Nitric oxide scavenging activity (NO) and Reduction of 1, 1-Diphenyl -2-Picryl Hydrazyl (DPPH) free radical scavenging activity. Throughout the studies the plant extract showed potent antioxidant activity and the activity was found to be concentration dependent which may be attributed to the high flavonoid content of the plant. Keywords: Cyanthillium cinereum (L) H.Rob, DPPH, NO.

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18

Ramadan Yasin, Mooner, Dler Jala Ramzan Sulaivani, and M. Hakki Alma. "The Extraction of Wild Mushrooms (Ganoderma Lucidum and Phellinus Torulosus) and Their Antioxidant Activities." International Journal of Research in Medical Sciences and Technology 14, no. 01 (2022): 32–37. http://dx.doi.org/10.37648/ijrmst.v14i01.005.

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The purpose of this study was to find DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities and the extract yields of two mushroom species, namely, Ganoderma lucidum (stored) and Phellinus torulosus (fresh). For the extraction of both mushroom bodies, methanol was used as solvent by using Blois extraction methods. The antioxidant activities of extracts were measured by UV-vis spectrophotometer at 517 nm. Butylated hydroxytoluene (BHT) was employed as a reference antioxidant. In conclusion, the highest DPPH scavenging activity (96.4%) was found in the body of Ganoderma lucidum while the lo

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19

Kırmızıbekmez, Hasan, Carla Bassarello, Sonia Piacente, et al. "Phenolic Compounds from Hypericum Calycinum and Their Antioxidant Activity." Natural Product Communications 4, no. 4 (2009): 1934578X0900400. http://dx.doi.org/10.1177/1934578x0900400418.

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From the MeOH extract of Hypericum calycinum, two caffeoylquinic acid derivatives, butyl chlorogenate (1), and chlorogenic acid (2), seven flavonoids, quercetin (3), quercitrin (4), hyperoside (5), isoquercitrin (6), miquelianin (7), rutin (8) and I3, II8-biapigenin (9) and two flavanols, (+)-catechin (10) and (-)-epicatechin (11) were isolated. Identification of the isolates was carried out by spectroscopic analysis including 1D and 2D NMR experiments as well as mass spectrometry. Free radical scavenging activities of the isolated compounds were determined in in-vitro 1,1-diphenyl-2-picrylhyd

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20

Wang, Kaiming, and Yingying Wang. "In Vitro Activity of Vernonia cumingiana Benth." Scientific Programming 2022 (March 30, 2022): 1–8. http://dx.doi.org/10.1155/2022/4151669.

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To investigate the antioxidant activity and antibacterial activity of Vernonia cumingiana Benth, in this study, spectrophotometry assays were used to determine the antioxidant and antibacterial activity indexes in vitro, including 1,1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging capacity and total antioxidant capacity, aiming to understand its pharmacodynamic components. The results showed that different component extracts had high DPPH scavenging ability in vitro, and the highest scavenging rate was obtained when chloroform extract of branches was 0.60 mg·mL−1, ethylene acetate

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21

Shatavisa, Mukherjee. "Exploring Antioxidant Potential of Some Common Marketed NonSteroidal Anti-Inflammatory Drugs." International Journal of Pharmaceutics & Pharmacology 1, no. 3 (2018): 112. https://doi.org/10.5281/zenodo.1160872.

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Considerable controversy exists on the effect of NSAIDs on the oxidative status. Despite being held pro-oxidant in few instances, there are many studies which have commented on the antioxidant effect of NSAIDs and its relevance and beneficial utilization in clinical set-up. The present study probed into the antioxidant potential of some common marketed NSAIDs in in-vitro set up using DPPH and Nitric oxide radical scavenging assay. Nitric oxide radical scavenging procedure showed maximum scavenging activity for paracetamol and mefenamic acid, followed by aspirin. Results from DPPH assay showed

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22

Siriwong, Suwimon, Adisai Rungvichaniwat, and Pairote Klinpituka. "Effect of Various Extracted Solvent on DPPH Radical Scavenging Activity of Natural Rubber." Key Engineering Materials 659 (August 2015): 388–93. http://dx.doi.org/10.4028/www.scientific.net/kem.659.388.

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Four types of extracted solvents i.e. mixtures of chloroform:acetone, chloroform:methanol, cyclohexane:acetone and cyclohexane:methanol, in the volume ratio of 4:1 were used to dissolve the natural rubber. Two grades of natural rubber i.e. air dry sheet (ADS) and ribbed smoked sheet No.3 (RSS3) were investigated. The rubber solution was purified by separated the rubber out with methanol. The non-rubber solution was then quantitative analyzed to verify 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity using Oxicount-Antioxidant kit test and also calculated the term of half maximal

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23

Zheng, Cheng-Dong, Gang Li, Hu-Qiang Li, Xiao-Jing Xu, Jin-Ming Gao, and An-Ling Zhang. "DPPH-Scavenging Activities and Structure-Activity Relationships of Phenolic Compounds." Natural Product Communications 5, no. 11 (2010): 1934578X1000501. http://dx.doi.org/10.1177/1934578x1000501112.

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Thirty-eight phenolic compounds (including 31 flavonoids) were examined for their DPPH radical-scavenging activities, and structure-activity relationships were evaluated. Specifically, the presence of an Ortho-dihydroxyl structure in phenolics is largely responsible for their excellent antiradical activity. 3-Hydroxyl was also essential to generate a high radical-scavenging activity. An increasing number of hydroxyls on flavones with a 3′,4′-dihydroxyl basic structure, the presence of a third hydroxyl group at C-5′, a phloroglucinol structure, glycosylation and methylation of the hydroxyls, an

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24

A. E., Vijaya Kumar, Vinay M. та Seethalakshami S. "Inhibition of free radical activity by dual PPAR α and PPAR γ agonist using analytical assay methods". International Journal of Basic & Clinical Pharmacology 6, № 7 (2017): 1670. http://dx.doi.org/10.18203/2319-2003.ijbcp20172728.

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Background: Overproduction of free radicals involved in the pathology of a wide variety of clinical disorders. Poor glycaemic control in diabetic people leads to free radical production responsible for diabetic related complications. Antioxidants produces resistance against the oxidative stress by scavenging free radicals may useful in treating diabetic related complications. Saroglitazar is a newer antidiabetic drug act on dual Peroxisome Proliferator Receptor Agonist α (PPAR α) and PPAR γ agonist with protection effect on Diabetes mellitus induced lipid dystrophy. Our study was done to evalu

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25

Verma, Neha, Vivekanand Katare, Nisha Kalme, Abhilasha Delouri, and Shivam Nema. "Evaluation of Antioxidant Activity of Trigonella foenum graecum Seeds Extract." Asian Journal of Dental and Health Sciences 3, no. 1 (2023): 11–14. http://dx.doi.org/10.22270/ajdhs.v3i1.33.

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In the current work, we completed a comprehensive assessment of the relative antioxidant activity in extracts from a few different medicinal plant species.The extracts' effectiveness at scavenging 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radicals was assessed spectrophotometrically. The highest degree of radical scavenging was 52.54%, while the IC50 value was 93.54.Trigonella foenum graecum extract demonstrates how the greater concentration of phytoconstituents chemicals leads to more powerful radical scavenging results. Keywords: Trigonella foenum graecum, Antioxidant, DPPH, Phytochemical analy

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26

Olanrewaju, O. A., and S. M. Auwal. "Effect of hydrolysis time and peptide content on dpph* radical scavenging activity of bromelain- and papain-generated hydrolysates from defatted cocoa beans (Theobroma cacao l.) powder." Bayero Journal of Pure and Applied Sciences 15, no. 1 (2022): 244–50. http://dx.doi.org/10.4314/bajopas.v15i1.33.

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Enzyme generated protein hydrolysates have received considerable attention due to their numerous health effects. Among these, the anti-oxidative hydrolysates with DPPH* radical scavenging activity can play a significant role in the prevention of oxidative stress and the resulting chronic disease conditions such as cancer. In the present study, defatted cocoa beans (DCB) powder was dialyzed and then hydrolyzed for 8 h to produce DCB protein hydrolysates using two different proteases (bromelain and papain) at an optimum pH (6.8), temperature (400C) and enzyme/substrate ratio (1:50). The resultin

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27

H, Rehana Banu, and Nagarajan N. "EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF A MEDICINAL HERB, WEDELIA CHINENSIS (OSBECK) MERRILL." Asian Journal of Pharmaceutical and Clinical Research 11, no. 10 (2018): 433. http://dx.doi.org/10.22159/ajpcr.2018.v11i10.25008.

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Objective: The objective is to evaluate the antioxidant activity of the methanolic leaf extract of Wedelia chinensis.Methods: In vitro antioxidant activity was evaluated by studying 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, superoxide radical scavenging activity, ferric reducing ability of plasma (FRAP) scavenging activity, hydroxyl radical scavenging activity, metal chelating activity, and total antioxidant activity. Ascorbic acid, BHT, quercetin, and EDTA were used as standards for the experiments.Results: Inhibition concentration50 values observed for DPPH radical sc

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28

Nurcholis, Waras, Nurul Khumaida, Muhamad Syukur, and Maria Bintang. "Evaluation of Free Radical Scavenging Activity in Ethanolic Extract from Promising Accessions of Curcuma aeruginosa RoxB." Molekul 12, no. 2 (2017): 133. http://dx.doi.org/10.20884/1.jm.2017.12.2.350.

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This study evaluated the free radical scavenging activity in ethanolic extracts from 20 accessions of Curcuma aeruginosa. The radical scavenging activity of the extract accessions was investigated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. IC50 values for DPPH radical scavenging activity ranged from 89.81 to 505.65 µg mL-1. Based on IC50 values, twenty accessions of C. aeruginosa can be divided into three groups: strong (two accessions); moderate (seventeen accessions); and low (one accession) of DPPH scavenger. Sukoharjo (SH) and Muara Bungo (MB) showed promising accessions for antiox

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Li, Qiong Jie, and Chun Ling Xiao. "Study on Antioxidant Activity of Pigment in Black Soybean Peel." Advanced Materials Research 881-883 (January 2014): 378–83. http://dx.doi.org/10.4028/www.scientific.net/amr.881-883.378.

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With black soybean as raw materials, 50% ethanol as the extractant, at 70°C, pH1.0, solid-liquid ratio of 1:100(g:mL), at 90min were used to extract the pigment of black soybean Through the establishment of DPPH•,•OH, superoxide anion free radicals, reducing power and hydrogen peroxide. The results show: its ability of scavenging DPPH•, •OH, hydrogen peroxide is far better than ascorbic acid. The pigment at concentration of 0.6mg/mL shows a scavenging rate for DPPH• up to 88.29%,and for •OH 89.36%,for hydrogen peroxide89.75%.In addition，the general reducing power and scavenging rate for supero

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30

Md. Mustafezur, Rahman, Saqueeb, Nazmus, Faruk, Md. Al, Rahman, Md. Arifu, and Sathi, Zakia Sultana. "Free radical scavenging Activity :." DIU Journal of Health and Life Sciences 1, no. 01 & 02 (2014): 137–40. http://dx.doi.org/10.36481/diuhls.v01i1-2.jchbx804.

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The present study was aimed at investigating the chemical profiles of the methanolic extract of leaves of Centella asiatica as well as to assess its antioxidative activity. In this study, a methanolic extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The phytochemical screening of the plant extract demonstrated the presence of several classes of compounds like alkaloids, flavonoids, tannins and steroids. The concentration of the plant extract needed for 50% scavenging (IC50) of DPPH was found to be 40.4 μg/ml whereas IC50values for the positive control was found t

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Rahman, Md Mustafezur, Nazmus Saqueeb, Md Al Faruk, Md Arifu Rahman, and Zakia Sultana Sathi. "Free radical scavenging Activity :." DIU Journal of Allied Health Sciences 1, no. 01 & 02 (2014): 137–40. https://doi.org/10.36481/diujahs.v01i1-2.jchbx804.

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The present study was aimed at investigating the chemical profiles of the methanolic extract of leaves of Centella asiatica as well as to assess its antioxidative activity. In this study, a methanolic extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The phytochemical screening of the plant extract demonstrated the presence of several classes of compounds like alkaloids, flavonoids, tannins and steroids. The concentration of the plant extract needed for 50% scavenging (IC50) of DPPH was found to be 40.4 μg/ml whereas IC50values for the positive control was found t

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32

Wu, Chun, Xin Ping Zheng, and Lin Lin Chen. "Study on Antioxidant Activity of Dihydromyricetin-Zinc(II) Complex." Advanced Materials Research 183-185 (January 2011): 863–67. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.863.

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Dihydromyricetin-zinc(II) complex was synthesized. The scavenging activity of DPPH radical, hydroxyl radical, superoxide anion radical and alkane radical by dihydromyricetin-zinc(II) complex were determine, moreover compared with Vc. The results showed that dihydromyricetin-zinc(II) complex possessed strong scavenging activities on DPPH•, hydroxyl radical, superoxide anion radical and alkane radical with correspounding maximum scavenging rate 74%, 70% , 65% and 50% at the concentration from 0.1 mmol/L to 0.5 mmol/L, respectively, which were higher than Vc. These results suggested that dihydrom

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33

Manoharan, Karuppiah Pillai, Isaac Santi Lehlohonolo, and Magama Sibusisiwe. "DPPH radical scavenging activity of extracts from Rhamnus prinoides." Journal of Medicinal Plants Research 13, no. 15 (2019): 329–34. http://dx.doi.org/10.5897/jmpr2019.6793.

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Mei, De Jun, Guo Ping Yu, and An Min Sun. "Preparation, Purification and Identification of Antioxidant Peptides with Bienzyme Hydrolysis from Rice Bran Protein." Advanced Materials Research 610-613 (December 2012): 72–80. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.72.

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The rice bran protein (RBP) was then hydrolyzed with various proteases (papain, flavorzyme, neutrase, protamex, and trypsin) to prepare antioxidant peptides. The rice bran protein hydrolysates (RBPH) were assessed using method of DPPH radical scavenging ability. Hydrolysate prepared with papain and flavorzyme (activity ratio 1:1) was found to have the highest antioxidant activity (IC50=6.778±0.21 mg/ml). This hydrolysate was purified using ultrafiltration, RBPH-III (Mw＜3KDa) had the highest DPPH and hydroxyl radical scavenging activity (IC50 value of 6.56±0.28, 5.43±0.22, respectively) and hig

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Rayadurgam, Sireesha, and Manikandan Krishnan. "Polyherbal Formulation for Atherosclerosis: GC-MS Fingerprinting, In-vivo Antioxidant and Antibacterial Activities." INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE 15, no. 01 (2024): 88–93. http://dx.doi.org/10.25258/ijpqa.15.1.13.

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Atherosclerosis is a prolonged inflammatory disease affecting large and medium-sized arteries, requiring a phytotherapeutic approach that incorporates multiple bioactive elements and a multi-target strategy. A novel polyherbal formulation has been formulated to treat atherosclerosis. The methanolic extract of the formulation has been characterized using gas chromatography–mass spectrometry (GC-MS) fingerprinting analysis. The extract was further screened for antioxidant activity like DPPH radical scavenging assay, hydrogen peroxide scavenging activity, intracellular reactive oxygen species (RO

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Chang, Min-Yun, Yin-Yi Lin, Yu-Chia Chang, et al. "Effects of Infusion and Storage on Antioxidant Activity and Total Phenolic Content of Black Tea." Applied Sciences 10, no. 8 (2020): 2685. http://dx.doi.org/10.3390/app10082685.

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This study determined antioxidant activity in terms of the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging ability and total phenolic content of black tea under different infusion and storage conditions. High performance liquid chromatography analysis identified caffeine, (−)-epigallocatechin, (−)-epicatechin-3-gallate, (−)-epigallocatechin-3-gallate and (−)-gallocatechin-3-gallate in the tea sample. The water–tea leaves weight ratio did not affect the DPPH scavenging ability. However, infusion temperature affected the DPPH scavenging activity and the total phenolic content. In the present stu

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Ahamd, Rahayu, Aisyah Mardhiyah Abd Ghafar, Silambarasi Mooralitharan, Imtinan Raihana Alfadino Akbar, and Nurul Farihan Mohamed. "Optimization of Extraction Temperature and Time for Antioxidant Lentinus Edodes using Response Surface Methodology." Semarak International Journal of Petroleum and Chemical Engineering 1, no. 1 (2024): 15–25. https://doi.org/10.37934/sijpce.1.1.1525a.

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The extraction conditions influencing the yield of crude polysaccharide and antioxidant activity in *Lentinus edodes* were studied and optimized using response surface methodology (RSM). Central composite design (CCD) was applied to optimize the extraction time and temperature. The results showed that the data were well-fitted to second-order polynomial models. The independent variables, temperature (A) and time (B), had significant linear, quadratic, and interaction effects on total carbohydrate content (TCC), total flavonoid content (TFC), and DPPH radical-scavenging activity. ANOVA analysis

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Abd Ghafar, Aisyah Mardhiyah, Silambarasi Mooralitharan, Imtinan Raihana Alfadino Akbar, Nurul Farihan Mohamed, and Rahayu Ahamd. "Optimization of Extraction Temperature and Time for Antioxidant Lentinus Edodes Using Response Surface Methodology." Semarak International Journal of Petroleum and Chemical Engineering 1, no. 1 (2024): 15–25. https://doi.org/10.37934/sijpce.1.1.1525.

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The extraction conditions influencing the yield of crude polysaccharide and antioxidant activity in *Lentinus edodes* were studied and optimized using response surface methodology (RSM). Central composite design (CCD) was applied to optimize the extraction time and temperature. The results showed that the data were well-fitted to second-order polynomial models. The independent variables, temperature (A) and time (B), had significant linear, quadratic, and interaction effects on total carbohydrate content (TCC), total flavonoid content (TFC), and DPPH radical-scavenging activity. ANOVA analysis

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Widyawati, Paini Sri. "DETERMINATION OF ANTIOXIDANT CAPACITY IN PLUCHEA INDICA LESS LEAVES EXTRACT AND ITS FRACTIONS." International Journal of Pharmacy and Pharmaceutical Sciences 8, no. 9 (2016): 32. http://dx.doi.org/10.22159/ijpps.2016v8i9.11410.

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<p><strong>Objective: </strong>This study was conducted to determine the DPPH free radical scavenging activity of pluchea extract (PE) and its fraction (ethyl acetate (EAF), n-butanol (BF) and aqueous (AF)) and comparing with the its activity of control antioxidant, such as green tea (GE) and rosemary extracts (RE), α-tocopherol succinate (ATS) and BHT.</p><p><strong>Methods</strong>:<strong> </strong>The antioxidant activity was evaluated by the decrease in absorbance as the result of DPPH˙ color change from purple to yellow at 517 nm by u

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Song, Jing Rong, and Gang Lv. "Antioxidant Activity of Fruits of Perillafrutescens." Applied Mechanics and Materials 644-650 (September 2014): 5262–65. http://dx.doi.org/10.4028/www.scientific.net/amm.644-650.5262.

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The antioxidant activities of extracts and residuum of Perillafrutescens fruits from supercritical CO2 extraction were determined in vitro. The residuum was extracted in turn with water, propyl alcohol and ethyl acetate. The antioxidant activities of the extracts were assayed with antioxidant capacity in linoleic acid model system, reducing powers, radical scavenging activity using 1,1-diphenyl-2-picrylhy-drazyl (DPPH) method. The results show that the ethyl acetate extract of Perillafrutescens possesses strongest DPPH radical scavenging activity and reducing power in a concentration-dependent

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Brodowska, Katarzyna Małgorzata, Agnieszka Joanna Brodowska, Krzysztof Śmigielski, and Elżbieta Łodyga-Chruścińska. "Antioxidant profile of essential oils and extracts of cinnamon bark (Cinnamomum cassia)." European Journal of Biological Research 6, no. 4 (2016): 310–16. https://doi.org/10.5281/zenodo.197200.

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Nowadays, more and more interest is focused on compounds that inhibit reactions promoted by free radicals. The study shows the antioxidant activity of essential oils isolated from <em>Cinnamomum cassia</em>. The methanolic extracts were also obtained and its scavenging ability investigated. The IC<sub>50</sub> value, defined as the concentration of the sample required for 50% scavenging of DPPH free radicals, was also estimated. DPPH and ABTS assay indicated that lower IC<sub>50</sub> value for cinnamon extracts corresponds to its higher radical scavenging activity (IC<sub>50</sub> = 42.03 µg/

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Yulyana, A., E. Mugiyanto, D. G. Guntara, et al. "Radical scavenging activity of ethanolic extract of green and red leaves of Cantigi (Vaccinium varingiaefolium Bl. Miq.)." Food Research 9, no. 2 (2025): 13–19. https://doi.org/10.26656/fr.2017.9(2).607.

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Indonesia's Cantigi (Vaccinium varingiaefoilum Bl. Miq.) is a member of the blueberry family that grows abundantly around volcanoes. It has two distinct leaf colors, red color leave (RCL) and green color leave (GCL), which contain secondary metabolites including anthocyanins. This study aimed to characterize the antioxidant properties of Cantigi leaf using various methods. DPPH (2,2’-diphenyl-1-picrylhydrazyl), BHA (butylhydroxyanisole), and BHT (butyl hydroxytoluene) were used to check the antioxidant activity of 96% ethanolic Cantigi extract. The phytochemical screening showed positive resul

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Venkatachalam, Suresh kumar Tirupur, SenthilKumar Palanisamy, Sudhagar Babu та Ranjithkumar Rajamani. "Assesment of free radical scavenging activity and inhibitory properties of aqueous extract of Cymodocea serrulata segrass towards α- glucosidase and α –amylase enzyme". Journal of University of Shanghai for Science and Technology 23, № 12 (2021): 339–60. http://dx.doi.org/10.51201/jusst/21/121035.

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Aim: The study reports the in vitro free radical scavenging and α-amylase and α-glucosidase inhibition activities of aqueous extract of seagrass Cymodocea serrulata from Gulf of Mannar, Southeast Asia. Methods: The free radical scavenging activity was evaluated by DPPH, Superoxide, Nitric oxide and Hydroxyl radical scavenging assays and α-amylase, α-glucosidase enzyme inhibitory activity. The C. serrulata showed effective DPPH radical scavenging activity of 69.45 μg/mL, Superoxide radical scavenging activity of 91.08.5 ± 0.02%, Nitric oxide radical scavenging activity of 86.89 ± 0.03% and Hydr

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Shu, Guowei, Qian Zhang, He Chen, Hongchang Wan, and Hong Li. "Effect Of Five Proteases Including Alcalase, Flavourzyme, Papain, Proteinase K And Trypsin On Antioxidative Activities Of Casein Hydrolysate From Goat Milk." Acta Universitatis Cibiniensis. Series E: Food Technology 19, no. 2 (2015): 65–74. http://dx.doi.org/10.1515/aucft-2015-0015.

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Abstract Oxidation was related to the pathogenesis of human diseases. Adequate intake of antioxidant activity of food can reduce the levels of free radicals, prevent lipid peroxidation, and help the body against diseases. In the paper, casein from goat milk was hydrolyzed by five commercial proteases, namely, Alcalase, flavourzyme, papain, proteinase K and trypsin. The antioxidant activities of casein hydrolysates were assessed by evaluating hydrolysis degree, DPPH radical-scavenging activity, metal-chelating activity and superoxide radical scavenging activity. The results showed as follows: t

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Susanti, Ni Made Pitri, Ni Kadek Warditian, and I. Made Agus Gelgel Wirasuta. "ANTIOXIDANT ACTIVITY TEST OF ANDROGRAPHOLIDE IN BITTER HERBS USING DPPH SCAVENGING." Journal of Health Sciences and Medicine 1, no. 1 (2017): 9. http://dx.doi.org/10.24843/jhsm.2017.v01.i01.p03.

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Abstract Bitter herbs (Sambiloto) have many pharmacological activities including antioxidant, antidiabetic, anticancer, antihyperlipidemic and anti-inflammation. Andrographolide is a diterpene compounds contained in bitter herbs. It is known that andrographolide compound responsible for the pharmacological activity of the bitter herbs. This study investigated DPPH free radical scavenging activity from andrographolide diterpene lactone. This study was initiated with the isolation of andrographolide compound from bitter herbs and then testing their DPPH free radical scave

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Baliyan, Siddartha, Riya Mukherjee, Anjali Priyadarshini, et al. "Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa." Molecules 27, no. 4 (2022): 1326. http://dx.doi.org/10.3390/molecules27041326.

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The use of F. religiosa might be beneficial in inflammatory illnesses and can be used for a variety of health conditions. In this article, we studied the identification of antioxidants using (DPPH) 2,2-Diphenyl-1-picrylhydrazylradical scavenging activity in Ficus religiosa, as F. religiosa is an important herbal plant, and every part of it has various medicinal properties such as antibacterial properties that can be used by the researchers in the development and design of various new drugs. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a popular, quick, easy, and affordable approach for the meas

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Xiao, Chun Ling, Ying Ren, and Hui Ling Ding. "Study on Extraction of Polyphenols from Black Soybeans and Antioxidant Activity." Applied Mechanics and Materials 675-677 (October 2014): 1629–33. http://dx.doi.org/10.4028/www.scientific.net/amm.675-677.1629.

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In this experiment black soybeans as raw materials,different factor on black beans polyphenol extraction yield influence,and studied the polyphenols antioxidant activity through establishing DPPH· system,·OH system and reducing power system,and compared with Vc. The result showed that:the optimum extraction conditions were the ratio of liquid to Solid was 32:1,ethanol concentration 48%,time 95min,temperature 90 °C.In these conditions, the yield of polyphenols was 8.953mg/g. The scavenging effects of polyphenols and Vc on DPPH·, ·OH force increased with increasing concentration,and the scavengi

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Enechi, Osmund Chukwuma Okeke Emmanuel Sunday* Abonyi Uchenna Collins Nwankwo Nicodemus Emeka Ndimele Ugochi Joy Benneth Moses Ebere. "EVALUATION OF IN VITRO ANTI-INFLAMMATORY AND ANTIOXIDANT POTENTIALS OF METHANOL LEAF EXTRACT OF FAGARA ZANTHOXYLOIDES." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES o6, no. 08 (2019): 14365–71. https://doi.org/10.5281/zenodo.3360753.

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<strong><em>Background:</em></strong><em> Fagara zanthoxyloides is a medicinal plant used in traditional medicine for the treatment of elephantiasis, toothache, sexual impotence, malaria, dysmenorrheal and abdominal pain among rural dwellers.</em> <strong><em>Objective:</em></strong><em> To study the In vitro anti-inflammatory and antioxidant activities of methanol extract of Fagara zanthoxyloides leaves.</em> <strong><em>Materials and Methods:</em></strong><em> Methanol extract of Fagara zanthoxyloides leaves were used for this study. In vitro anti-inflammatory studies were performed for the

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Swathi, Naraparaju, and Thirumurthy Durai Ananda Kumar. "Insights into Thiazolidinedione Analogues: Unveiling Antioxidant Activity through Descriptor-Based Quantitative Structure-Activity Relationship Investigations." Asian Journal of Applied Chemistry Research 15, no. 1 (2024): 1–11. http://dx.doi.org/10.9734/ajacr/2024/v15i1277.

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Background: Diabetes mellitus, a chronic metabolic disorder is characterized by defective insulin secretion (β-cell dysfunction), insulin action (insulin resistance) and reduced bio-antioxidant potential. Antioxidants play key role in diabetes by reducing the oxidative stress and alleviating diabetic complications. Thiazolidinediones (TZDs) attenuate insulin resistance and produce antioxidant effect. Study Design: In continuation of our goal to develop thiazolidine-2,4-dione analogues that can address both oxidative stress and Type 2 diabetes, the in vitro antioxidant potential of a few synthe

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Wang, Xiang, Guang-Lei Zuo, Chao-Yue Wang, Hyun Yong Kim, Soon Sung Lim, and Sheng-Qiang Tong. "An Off-Line DPPH-GC-MS Coupling Countercurrent Chromatography Method for Screening, Identification, and Separation of Antioxidant Compounds in Essential Oil." Antioxidants 9, no. 8 (2020): 702. http://dx.doi.org/10.3390/antiox9080702.

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Essential oils are an important source of natural antioxidants and multiple methods have been established for evaluation of their overall antioxidant activity, however, the antioxidant activities of their compounds are less investigated. In the present study, the hyphenation of 2,2′-diphenyl-1-picrylhydrazyl (DPPH)-gas chromatography (GC)-mass spectrometry (MS) offline and high-speed countercurrent chromatography (HSCCC) is established for efficient screening, identification, and isolation of antioxidants from essential oils and applied to the essential oil of Curcuma wenyujin Y.H. Chen et C.

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