Relevant bibliographies by topics / Droplet based microfluidic

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers
  5. Reports

Academic literature on the topic 'Droplet based microfluidic'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 February 2022

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Journal articles on the topic "Droplet based microfluidic"

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Gao, Zehang, Huo Peng, Minjie Zhu, Lei Wu, Chunping Jia, Hongbo Zhou, and Jianlong Zhao. "A Facile Strategy for Visualizing and Modulating Droplet-Based Microfluidics." Micromachines 10, no. 5 (April 29, 2019): 291. http://dx.doi.org/10.3390/mi10050291.

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In droplet-based microfluidics, visualizing and modulating of droplets is often prerequisite. In this paper, we report a facile strategy for visualizing and modulating high-throughput droplets in microfluidics. In the strategy, by modulating the sampling frequency of a flash light with the droplet frequency, we are able to map a real high frequency signal to a low frequency signal, which facilitates visualizing and feedback controlling. Meanwhile, because of not needing synchronization signals, the strategy can be directly implemented on any droplet-based microfluidic chips. The only cost of the strategy is an additional signal generator. Moreover, the strategy can catch droplets with frequency up to several kilohertz, which covers the range of most high-throughput droplet-based microfluidics. In this paper, the principle, setup and procedure were introduced. Finally, as a demonstration, the strategy was also implemented in a miniaturized picoinjector in order to monitor and control the injection dosage to droplets. We expect that this facile strategy supplies a low-cost yet effective imaging system that can be easily implemented in miniaturized microfluidic systems or general laboratories.
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Dressler, Oliver J., Richard M. Maceiczyk, Soo-Ik Chang, and Andrew J. deMello. "Droplet-Based Microfluidics." Journal of Biomolecular Screening 19, no. 4 (November 15, 2013): 483–96. http://dx.doi.org/10.1177/1087057113510401.

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Over the past two decades, the application of microengineered systems in the chemical and biological sciences has transformed the way in which high-throughput experimentation is performed. The ability to fabricate complex microfluidic architectures has allowed scientists to create new experimental formats for processing ultra-small analytical volumes in short periods and with high efficiency. The development of such microfluidic systems has been driven by a range of fundamental features that accompany miniaturization. These include the ability to handle small sample volumes, ultra-low fabrication costs, reduced analysis times, enhanced operational flexibility, facile automation, and the ability to integrate functional components within complex analytical schemes. Herein we discuss the impact of microfluidics in the area of high-throughput screening and drug discovery and highlight some of the most pertinent studies in the recent literature.
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Duchamp, Margaux, Marion Arnaud, Sara Bobisse, George Coukos, Alexandre Harari, and Philippe Renaud. "Microfluidic Device for Droplet Pairing by Combining Droplet Railing and Floating Trap Arrays." Micromachines 12, no. 9 (September 6, 2021): 1076. http://dx.doi.org/10.3390/mi12091076.

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Droplet microfluidics are characterized by the generation and manipulation of discrete volumes of solutions, generated with the use of immiscible phases. Those droplets can then be controlled, transported, analyzed or their content modified. In this wide droplet microfluidic toolbox, no means are available to generate, in a controlled manner, droplets co-encapsulating to aqueous phases. Indeed, current methods rely on random co-encapsulation of two aqueous phases during droplet generation or the merging of two random droplets containing different aqueous phases. In this study, we present a novel droplet microfluidic device to reliably and efficiently co-encapsulate two different aqueous phases in micro-droplets. In order to achieve this, we combined existing droplet microfluidic modules in a novel way. The different aqueous phases are individually encapsulated in droplets of different sizes. Those droplet populations are then filtered in order to position each droplet type towards its adequate trapping compartment in traps of a floating trap array. Single droplets, each containing a different aqueous phase, are thus paired and then merged. This pairing at high efficiency is achieved thanks to a unique combination of floating trap arrays, a droplet railing system and a droplet size-based filtering mechanism. The microfluidic chip design presented here provides a filtering threshold with droplets larger than 35 μm (big droplets) being deviated to the lower rail while droplets smaller than 20 μm (small droplets) remain on the upper rail. The effects of the rail height and the distance between the two (upper and lower) rails were investigated. The optimal trap dimensions provide a trapping efficiency of 100% for small and big droplets with a limited double trapping (both compartments of the traps filled with the same droplet type) of 5%. The use of electrocoalescence enables the generation of a droplet while co-encapsulating two aqueous phases. Using the presented microfluidic device libraries of 300 droplets, dual aqueous content can be generated in less than 30 min.
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Ki, Sunghyun, and Dong-Ku Kang. "Gas Crosstalk between PFPE–PEG–PFPE Triblock Copolymer Surfactant-Based Microdroplets and Monitoring Bacterial Gas Metabolism with Droplet-Based Microfluidics." Biosensors 10, no. 11 (November 11, 2020): 172. http://dx.doi.org/10.3390/bios10110172.

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The PFPE–PEG–PFPE (Perfluoropolyether-polyethylene glycol-perfluoropolyether) surfactant has been used in droplet-based microfluidics and is known to provide high droplet stability and biocompatibility. Since this surfactant ensures the stability of droplets, droplet-based microfluidic systems have been widely used to encapsulate and analyze various biological components at the single-molecule scale, including viruses, bacteria, nucleic acids and proteins. In this study, we experimentally confirmed that gas crosstalk occurred between droplets formed by fluorinated oil and the PFPE–PEG–PFPE surfactant. E. coli K-12 bacterial cells were encapsulated with Luria–Bertani broth within droplets for the cultivation, and gas crosstalk was identified with neighboring droplets that contain phenol red. Since bacteria produce ammonia gas during its metabolism, penetration of ammonia gas initiates a color change of phenol red-containing droplets. Ammonia gas exchange was also confirmed by reacting ammonium chloride and sodium hydroxide within droplets that encapsulated. Herein, we demonstrate the gas crosstalk issue between droplets when it is formed using the PFPE–PEG–PFPE surfactant and also confirm that the density of droplet barrier has effects on gas crosstalk. Our results also suggest that droplet-based microfluidics can be used for the monitoring of living bacteria by the determination of bacterial metabolites during cultivation.
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Cliffe, Finola E., Mark Lyons, Daniel C. Murphy, Lisa McInerney, Niall Hurley, Michael A. Galvin, Jane Mulqueen, et al. "Droplet Combinations: A Scalable Microfluidic Platform for Biochemical Assays." SLAS TECHNOLOGY: Translating Life Sciences Innovation 25, no. 2 (October 30, 2019): 140–50. http://dx.doi.org/10.1177/2472630319883830.

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Droplet-based microfluidics holds enormous potential for transforming high-throughput drug screening. Miniaturization through droplets in combination with automation contributes to reduce reagent use and analysis time as well as minimizing or eliminating labor-intensive steps leading to associated reductions in cost. In this paper, we demonstrate the potential of automated and cost-effective microfluidic droplet-generating technology in the context of an enzymatic activity assay for screening collagenase inhibitors. Experimental results show reproducible and accurate creation and mixing of droplet combinations resulting in biochemical data comparable to data produced by an industry standard instrument. This microfluidic platform that can generate and combine multiple droplets represents a promising tool for high-throughput drug screening.
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Luo, Zhijie, Bangrui Huang, Jiazhi Xu, Lu Wang, Zitao Huang, Liang Cao, and Shuangyin Liu. "Machine vision-based driving and feedback scheme for digital microfluidics system." Open Chemistry 19, no. 1 (January 1, 2021): 665–77. http://dx.doi.org/10.1515/chem-2021-0060.

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Abstract A digital microfluidic system based on electrowetting-on-dielectric is a new technology for controlling microliter-sized droplets on a plane. By applying a voltage signal to an electrode, the droplets can be controlled to move, merge, and split. Due to device design, fabrication, and runtime uncertainties, feedback control schemes are necessary to ensure the reliability and accuracy of a digital microfluidic system for practical application. The premise of feedback is to obtain accurate droplet position information. Therefore, there is a strong need to develop a digital microfluidics system integrated with driving, position, and feedback functions for different areas of study. In this article, we propose a driving and feedback scheme based on machine vision for the digital microfluidics system. A series of experiments including droplet motion, merging, status detection, and self-adaption are performed to evaluate the feasibility and the reliability of the proposed scheme. The experimental results show that the proposed scheme can accurately locate multiple droplets and improve the success rate of different applications. Furthermore, the proposed scheme provides an experimental platform for scientists who focused on the digital microfluidics system.
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Kalantarifard, Ali, Abtin Saateh, and Caglar Elbuken. "Label-Free Sensing in Microdroplet-Based Microfluidic Systems." Chemosensors 6, no. 2 (May 24, 2018): 23. http://dx.doi.org/10.3390/chemosensors6020023.

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Droplet microfluidic systems have evolved as fluidic platforms that use much less sample volume and provide high throughput for biochemical analysis compared to conventional microfluidic devices. The variety of droplet fluidic applications triggered several detection techniques to be applied for analysis of droplets. In this review, we focus on label-free droplet detection techniques that were adapted to various droplet microfluidic platforms. We provide a classification of most commonly used droplet platform technologies. Then we discuss the examples of various label-free droplet detection schemes implemented for these platforms. While providing the research landscape for label-free droplet detection methods, we aim to highlight the strengths and shortcomings of each droplet platform so that a more targeted approach can be taken by researchers when selecting a droplet platform and a detection scheme for any given application.
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Rho, Hoon Suk, and Han Gardeniers. "Microfluidic Droplet-Storage Array." Micromachines 11, no. 6 (June 23, 2020): 608. http://dx.doi.org/10.3390/mi11060608.

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A microfluidic droplet-storage array that is capable of the continuous operation of droplet formation, storing, repositioning, retrieving, injecting and restoring is demonstrated. The microfluidic chip comprised four valve-assisted droplet generators and a 3 × 16 droplet-storage array. The integrated pneumatically actuated microvalves enable the precise control of aqueous phase dispensing, as well as carrier fluid flow path and direction for flexible manipulating water-in-oil droplets in the chip. The size of droplets formed by the valve-assisted droplet generators was validated under various operating conditions such as pressures for introducing solutions and dispensing time. In addition, flexible droplet addressing in the storage array was demonstrated by storing droplets with various numbers and compositions in different storage units as well as rearranging their stored positions. Moreover, serial injections of new droplets into a retrieved droplet from a storage unit was performed to show the potential of the platform in sequential dosing on incubated droplet-based reactors at the desired timeline. The droplet-storage array with great freedom and flexibility in droplet handling could be applied for performing complex chemical and biologic reactions, especially in which incubation and dosing steps are necessary.
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Candoni, Nadine, Romain Grossier, Mehdi Lagaize, and Stéphane Veesler. "Advances in the Use of Microfluidics to Study Crystallization Fundamentals." Annual Review of Chemical and Biomolecular Engineering 10, no. 1 (June 7, 2019): 59–83. http://dx.doi.org/10.1146/annurev-chembioeng-060718-030312.

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This review compares droplet-based microfluidic systems used to study crystallization fundamentals in chemistry and biology. An original high-throughput droplet-based microfluidic platform is presented. It uses nanoliter droplets, generates a chemical library, and directly solubilizes powder, thus economizing both material and time. It is compatible with all solvents without the need for surfactant. Its flexibility permits phase diagram determination and crystallization studies (screening and optimizing experiments) and makes it easy to use for nonspecialists in microfluidics. Moreover, it allows concentration measurement via ultraviolet spectroscopy and solid characterization via X-ray diffraction analysis.
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Sánchez Barea, Joel, Juhwa Lee, and Dong-Ku Kang. "Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology." Micromachines 10, no. 6 (June 20, 2019): 412. http://dx.doi.org/10.3390/mi10060412.

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Recently, droplet-based microfluidic systems have been widely used in various biochemical and molecular biological assays. Since this platform technique allows manipulation of large amounts of data and also provides absolute accuracy in comparison to conventional bioanalytical approaches, over the last decade a range of basic biochemical and molecular biological operations have been transferred to drop-based microfluidic formats. In this review, we introduce recent advances and examples of droplet-based microfluidic techniques that have been applied in biochemistry and molecular biology research including genomics, proteomics and cellomics. Their advantages and weaknesses in various applications are also comprehensively discussed here. The purpose of this review is to provide a new point of view and current status in droplet-based microfluidics to biochemists and molecular biologists. We hope that this review will accelerate communications between researchers who are working in droplet-based microfluidics, biochemistry and molecular biology.
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Dissertations / Theses on the topic "Droplet based microfluidic"

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Martino, Chiara. "Droplet-based microfluidic platforms for protein investigations." Thesis, University of Glasgow, 2013. http://theses.gla.ac.uk/4005/.

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In the last two decades, the integration of life science and micro-engineering has developed systems which are able to perform laboratory functions on devices only 10-100 μm in size. These microfluidic systems, called lab-on-chip (LOC), show promising capabilities in reducing both the time and the cost of a wide range of chemical and biological processes. More recently, the creation of microfluidic systems which are able to form and control sub-nanolitre droplets, comprising two phase emulsions, have been developed to deliver new experimental platforms. Such systems, also known microdroplets or segmented flow platforms, consist of stable liquid droplets, suspended in a second immiscible phase, with volumes on the nanolitre to the femtolitre scale. The potential of these systems within chemical and biological sciences has already been clearly demonstrated in the literature and commercial platforms are now becoming available. Some of the appealing features that these systems allow are the compartmentalization, the ultra-high throughput experimentation, the imitation of cellular conditions in terms of volumes and chemical composition. The aim of this research project is to exploit the droplet-based LOC systems using the two phase segmented flow to create platforms where proteins can be investigated and even expressed within the droplet chassis. Initial work used a single emulsion strategy (i.e. droplet of water in oil) to selectively capture cellular proteins from a cell suspension, which was directly processed on chip. It was observed that proteins remain active in these systems. In addition, a complexity of conventional laboratory procedures for protein quantification assays was reduced, both in terms of investigation times and amounts of valuable biological samples used. The obtained results demonstrated that this system has the potential to provide the same level of quantitative information obtained using standard biological techniques (i.e. Western blot) at a lower cost. The research has been moving over the development of artificial cell models, nanolitre sized watery droplets comprising a membrane separating the inner from the external environment. Within these systems, realised using a double emulsion strategy (i.e. droplet of water with a droplet of oil or other immiscible phase surrounded by another watery phase), proteins have been expressed using cell-free protein expression systems. The technology adopted lies broadly within the field of synthetic biology involving the transformation of microorganism’s DNA for the production of the desired proteins. Fluorescent proteins were designed and expressed within the artificial cell and fluorescence assays, implemented within the microfluidic format, confirmed not only the functionality of the expressed protein which behaved like in vivo, but also the possibility of its controlled release. Protein release was possible through the use of polymers, within the double emulsion format, which represent a good class of material for the production of nanometre thickness shells. Future developments in this research will aim to (i) expand the capabilities of the single emulsion system for the capture of multiple proteins from a cell lysate and (ii) use different class of polymers to enrich the artificial cell membrane with membrane proteins aiming towards the natural cell mimicking. Emulsions capable to mimic some aspects of the living cell, like the protein synthesis, represent a great opportunity to be used as tools for protein investigations.
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Guermonprez, Cyprien. "Droplet-based Microfluidic Platform for Quantitative Microbiology." Thesis, Université Paris-Saclay (ComUE), 2016. http://www.theses.fr/2016SACLX106/document.

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Développement d'une plateforme microfluidique pour la microbiologie quantitative. La plateforme permet la culture de milliers de colonies en parallèle dans des micro-gouttes. L'utilisation de tableau statique pour stocker les gouttes permet non seulement leur observation dans le temps pour des analyses dynamiques mais également la récupération de n'importe quelle goutte pour des études complémentaires. Nous avons également développé un outil permettant de soumettre les gouttes à des gradients chimiques directement sur la plateforme dont nous présentons les mécanismes physiques. Nous avons développé un software d'analyse des données générées par la plateforme pour l'étude de modèles de croissance bactérienne ainsi que l'impact des antibiotiques sur leur prolifération
Development of a microfluidic chip for quantitative microbiology. The chip allow for parallel culture of thousands bacterial colonies in micro-droplets stored in static array. The 2D-array enable not only the visualisation of each colonies in timelapse experiment but also the extraction of any of them out of the chip at any time for further analysis (PCR, re-culture,...). The platform is adaptable to a concentration gradient producer, for which we present the physical understanding of working mechanism, that can apply different chemical environments to each colony. We developed in parallel a software that perform the analysis of the data generated by the platform to adress bacteria growth studies as well as the impact of antibiotics on bacteria proliferation
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Schulz, Martin [Verfasser], and Felix von [Akademischer Betreuer] Stetten. "Microfluidic system integration for droplet based digital nucleic acid testing." Freiburg : Universität, 2020. http://d-nb.info/1229349278/34.

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Cavazos, Omar. "Microfluidic-Based Fabrication of Photonic Microlasers for Biomedical Applications." Thesis, University of North Texas, 2019. https://digital.library.unt.edu/ark:/67531/metadc1609066/.

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Optical microlasers have been used in different engineering fields and for sensing various applications. They have been used in biomedical fields in applications such as for detecting protein biomarkers for cancer and for measuring biomechanical properties. The goal of this work is to propose a microfluidic-based fabrication method for fabricating optical polymer based microlasers, which has advantages, over current methods, such us the fabrication time, the contained cost, and the reproducibility of the microlaser's size. The microfluidic setup consisted of microfluidic pumps and a flow focusing droplet generator chip made of polydimethylsiloxane (PDMS). Parameters such as the flow rate (Q) and the pressure (P) of both continuous and dispersed phases are taken into account for determining the microlaser's size and a MATLAB imaging tool is used to reduce the microlaser's diameter estimation. In addition, two applications are discussed: i) electric field measurements via resonator doped with Di-Anepps-4 voltage sensitive dye, and ii) strain measurements in a 3D printed bone-like structure to mimic biomedical implantable sensors.
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Leung, Kaston. "A programmable droplet-based microfluidic device for multiparameter single-cell analysis." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/43855.

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Bhattacharjee, Biddut. "Study of droplet splitting in an electrowetting based digital microfluidic system." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/43314.

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This thesis focuses on the symmetric and asymmetric splitting of droplets, which is a prominent fluidic operation in a digital microfluidic system (DMFS). The prerequisite part of the investigation of droplet splitting is to understand the electrowetting-on-dielectric (EWOD) based droplet actuation. This thesis demonstrates that not only the EWOD actuation is a self-feedback system - implying that the actuation force depends on the position of the droplet, but also the size of the droplet affects the magnitude of actuation force. However, a sensing mechanism is essential for complex operations, e.g. dispensing and splitting. One contribution of this thesis is a novel method of sensing the droplet position that requires connections to the two adjacent electrodes in the lower plate only. For the fabrication of prototype DMFS, a new polymeric material, cyanoethyl pullulan (CEP), is proposed as the dielectric layer resulting in a simple and low-cost fabrication of DMFS. The required voltage for droplet manipulation is drastically reduced owing to high relative permittivity of CEP. Droplet splitting is investigated both numerically and experimentally. Numerical investigation of droplet splitting in FLOW-3D®, a commercial computational fluid dynamics software, revealed that the strength of viscous forces relative to the surface tension force determines the success of splitting. For successful asymmetric splitting, performed by applying voltages of unequal magnitude to left-hand and right-hand sides of the droplet, there exists a minimum voltage for the low-voltage side that guarantees splitting. This minimum voltage increases if the aspect ratio (i.e., diameter to height) of the droplet is reduced while keeping the diameter of the droplet constant. Investigation of the asymmetric splitting with different ratios of applied voltage revealed that the ratio between the volumes of accumulated liquid on either sides increases with voltage ratio. The feasibility of asymmetric splitting as well as the effects of different ratios of applied voltages were studied in prototype DMFS. The results verify the existence of a minimum voltage for successful splitting. The ratio between the volumes of the sister droplets increases with that of the applied voltages. Moreover, the general characteristics of flow-rates and liquid accumulation were found to be similar to those in simulations.
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Lu, Heng. "Development of droplet-based microfluidic tools for toxicology and cancer research." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCB064.

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Ce projet de thèse portait sur le développement d’outils microfluidiques pour la toxicologie et la recherche contre le cancer. En permettant l’analyse simultanée d’un très grand nombre de réactions biologiques ou chimiques réalisés dans des compartiments indépendants (ie. gouttelettes), la microfluidique de gouttes offre une sensibilité de détection et une précision sans précédent pour l’analyse de molécules biologiques, telles que l’ADN ou les Anticorps, en comparaison des expériences réalisées conventionnellement en tubes ou en microplaques (essais en « bulk » ou volume). Ce format permet également de réaliser des expériences à très haut débit et est particulièrement pertinent pour la toxicologie, où des analyses robustes de l’effet des médicaments sont nécessaires. De même, ces procédures sont également très adaptées à l’analyse de cellules uniques pour le séquençage ADN ou ARN et l’épigénomique. Tout cela fait de la microfluidique en goutte un outil puissant pour la toxicologie et la recherche sur le cancer. En premier temps, une méthode du comptage précise des cellules encapsulée dans des microgouttelettes, nommée « hémocytométrie microfluidique », a été développée. Un nouvel algorithme de comptage a été proposé. Des cellules bactériennes (Escherichia Coli) et des cellules de 2 lignées humaines différentes (HL60 and H1975) ont été testées. Le nombre de chaque type de cellules a été déterminé avec une haute corrélation entre la théorie (basée sur la distribution de Poisson) et les résultats expérimentaux. Avec ces résultats robustes, un protocole de microfluidique en goutte a été mis en place pour interroger la viabilité cellulaire et la prolifération des 2 lignées humaines. Ces résultats sont en concordance avec ceux de la littérature. Pour la toxicologie, 3 différents modèles, y compris des microsomes (extrait de cellules d’insectes infectées par un baculovirus exprimant le cytochrome P450 3A4 humain, CYP3A4), HepG2-CYP3A4 (modifiée génétiquement pour exprimer le gène CYP3A4 humain), et HepaRG, une lignée hépatique, ont été évaluées pour l’activité enzymatique du CYP3A4, une enzyme largement utilisée en routine pour le criblage de médicament candidat. Les microsomes ont permis de développer un essai fluorogénique permettant de mesurer l’inhibition du CYP3A4. Cependant, ni l’utilisation des microsomes ni des cellules HepG2 exprimant CYP3A4 n’a donné de résultats satisfaisants en microgouttelettes. L’utilisation des cellules HepaRG, une lignée cellulaire qui conserve la majorité de l’expression des cytochromes P450 et des récepteurs nucléaires nécessaire à leur expression, a montré des résultats encourageant à la fois sur les tests de mesure de l’activité enzymatique et d’analyse de l’induction du CYP3A4. Pour la recherche sur le cancer, 4 essais originaux de PCR digitale en gouttes ont été mis en place pour la détection et la quantification de mutations (NRAS, DNMT3A, SF3B1 and JAK2) importante pour les syndromes myélodysplasiques, un groupe hétérogène de maladies touchant les cellules souches hématopoïétiques caractérisées par une hématopoïèse inefficace et des cytopénies périphériques. Finalement, un essai de PCR sur cellule unique encapsulées au sein de billes agarose a été proposé
This thesis project consists in developing droplet-based microfluidic tools for toxicology and cancer research. Owing to its large numbers of discretized volumes, sensitivity of detection of droplet-based microfluidics for biological molecules such as DNA and antibody is much higher than bulk assays. This high throughput format is particularly suitable for experiments where a robust dose-response curve is needed, as well as for single cell analysis with applications in genomic or sequencing and epigenetics. All above makes droplet-based microfluidics a powerful tool for toxicology and cancer research. In a first part of the work, an accurate cell counting method, named “microfluidics hemocytometry”, has been developed. A new counting algorithm was proposed to count the cells within each droplet. Escherichia Coli and two different human cell lines (HL60 and H1975) were used to validate our strategy. The number of each type of cells in droplets was determined with a high consistency between theory (Poisson distribution) and experimental results. With these robust results, a droplet-based microfluidic protocol has then been established to inquiry both cell viability and proliferation for the two human cell lines. The results are in good agreement with the one of the literature. For the toxicology, 3 different biological models, including microsomes (extracted from baculovirus-infected insect cell expressing human CYP3A4), HepG2-CYP3A4 (genetically modified to express the human CYP3A4 gene) and HepaRG liver cells lines were evaluated for enzymatic activity of cytochromes P450 (CYP3A4), a routinely used enzyme for drug candidate screening. Microsome-based assays were used to validate a fluorogenic inhibition assay. However neither microsome-based assay nor the assay using CYP3A4 expressing HepG2 gave satisfying results in droplet-based format. However, HepaRG cells, a hepatic function-conserved cell line with most cytochrome and related nuclear receptors, demonstrated high relevance both for enzymatic activity testing and CYP3A4 expression induction study. For cancer research, 4 different picoliter droplet-based PCR assays were developed for the detection and quantification of mutations (NRAS, DNMT3A, SF3B1 and JAK2) present in Myelodysplastic syndromes, a heterogeneous group of clonal bone marrow hematopoietic stem cell disorders characterized by ineffective hematopoiesis and peripheral cytopenias. Furthermore, a single cell multistep PCR assay using encapsulation of target DNA in agarose droplets was proposed
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Pekin, Deniz. "Development of novel droplet-based microfluidic strategies for the molecular diagnosis of cancer." Phd thesis, Université de Strasbourg, 2013. http://tel.archives-ouvertes.fr/tel-00856594.

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The aim of this work is to establish novel strategies for the highly sensitive screening of cancer biomarkers in biological samples.To achieve this goal, we developed droplet-based microfluidic dPCR technique. Using a limiting dilution, individual DNA molecules are encapsulated within monodisperse droplets of a water-in-oil emulsion created with a microfluidic device. Fluorescent TaqMan® probes targeting the screened cancer biomarkers allow the detection of mutations. We focused on the mutations in the human KRAS gene for the development of our test. This method is also transposable in a multiplexed format for the parallel detection of multiple mutations in clinical samples.The developed technique allowed the precise quantification of a mutated KRAS gene in the presence of a 200,000-fold excess of un-mutated KRAS genes and enabled the determination of mutant allelic specific imbalance (MASI) in several cancer cell-lines. We validated our technique by screening for KRAS mutations in the blood plasma and tumor samples from patients with metastatic colorectal cancer.
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Cho, Soongwon David. "An integrated droplet based microfluidic platform for high throughput, multi-parameter screening of photosensitiser activity." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/18482.

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With rapid advances in the field of cellomics, genomics, and proteomics, the demands for development of enabling technologies for performing high throughput biological experimentation are ever increasing. Droplet based microfluidic systems have recently been developed to perform high throughput experimentations. With the ability to generate droplets over 1 kHz frequency and perform combinatorial experiments via various passive and active manipulating techniques, microdroplet technology provides an ideal platform for combinatorial biological experiments whilst consuming minimal amount of reagent. As it is possible to generate droplets, manipulate them, and characterise droplets using highly sensitive on-line detection systems, it is now crucial to bring various functionalities together to create a micro total analysis system capable of performing complex biological experiments within microfluidic devices. As such, an integrated droplet based microfluidic platform was developed to assess the efficacy of photodynamic therapy against microbial organisms. Photodynamic therapy is an alternative efficacious treatment method for the treatment of localized microbial infections with several favourable features such as broad spectrum of action, efficient inactivation of multidrug-resistant bacteria, and low mutagenic potential. In order to perform the photosensitiser cytotoxicity screening, various microfluidic modules such as droplet generation, chamber based microdroplet storage and light irradiation, droplet reinjection, electrocoalescence and on-chip viability scoring of cells within droplets using a combination of carboxyfluorescein diacetate and propidium iodide were developed and integrated within the microfluidic platform. The microfluidic system was then used to screen the cytotoxicity of TBO against E.coli cells and the results were validated against conventional colony forming unit assays. Finally, the integrated system was used to assess the effects of several parameters on E.coli viability such as dark toxicity, photosensitiser concentration, light dose and poor oxygenation condition.
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Ghifari, Najla. "Microfluidic-based colloidal ZnO microcapsules : synthesis, structure,organization and first applications." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPAST066.

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Ce travail se concentre sur le développement d’une approche microfluidique originale à base degouttelettes pour générer des sphères de ZnO de taille micrométrique très monodispersées avec une taille et une morphologie bien contrôlées. Cette approche est simple et prometteuse non seulement pour la fabrication de microcapsules de ZnO de taille uniforme, avec une taille ajustable et un contrôle précis à l’échelle micrométrique, mais aussi pour acquérir de nouvelles connaissances sur la compréhension des processus de croissance colloïdale et l’auto-organisation des nanoparticules de ZnO par la voie microfluidique. En outre, ces microparticules peuvent trouver des applications intéressantes dans de nombreux domaines tels que la photonique, le photovoltaïque ou la biomédecine. Ce travail porte sur l’effet des paramètres de fabrication sur la formation de gouttelettes, la taille et la stabilité des microsphères résultantes, ainsi que sur l’étude de leurs propriétés optiques et électriques, encouplant les travaux expérimentaux et théoriques. Nous avons montré la synthèse, dans une gamme micrométrique allant de 10 µm à 30 µm, de microcapsules de ZnO mésoporeuses à enveloppe fine et flexible. Nous étudions la caractéristique polaire des nanoparticules de ZnO et leur auto-organisation interfaciale. En outre, nous révélons que les charges électriques portées par lesunités primaires de ZnO jouent un rôle crucial dans la stabilité des gouttelettes en présence et en absence de molécules chargées. Elle joue également un rôle clé tout au long du processus d’assemblage, de la création des nanoparticules colloïdales de ZnO aux microgouttelettes, et enfin aux microsphères. Nous rapportons, pour la première fois, l’auto-organisation de microgouttelettes de ZnO liquide dopé en réseaux carrés. Nous démontrons qu’un tel résultat révèle l’aspect polaire des microgouttelettes de ZnO et corrobore un changement d’équilibre entre les forces motrices contrôlant l’organisation des nanoparticules de ZnO à l’échelle nanométrique. Nous avons développé différents modèles, en très bon accord avec le champdipolaire et les mécanismes de forces interfaciales, pour étayer les résultatsexpérimentaux mis en avant, et pour expliquer l’organisation interfaciale des nanoparticules de ZnO/RhB sur la base des propriétés d’organisation des gouttelettes de ZnO. À partir de nos résultats et de la dépendance constatée de la taille des microcapsules, de l’épaisseur de la coquille et de la densité de surface des nanoparticules par rapport à la taille des gouttelettes, nous fournissons un modèle original de la contribution des facteurs impliqués dans le mécanisme de formation de la coquille
This work focuses on the development of an original droplet-based microfluidics approach to generate highly monodisperse micrometer-sized ZnO spheres with well-controlled size and morphology. This approach is straightforward, and promising not only for the fabrication of uniform-sized ZnO microcapsules, with adjustable size and precise control at the microscale, but also for gaining new insights into the understanding of colloidal growth processes and self-organization of ZnO nanoparticles by the microfluidic route. In addition, such microparticles may find interesting applications in many areas such as photonics, photovoltaics, or biomedecine. This work deals with the effect of handling parameters on droplet formation, size, and stability of the resulting microspheres, as well as the study of their optical and electrical properties coupling experimental and theoretical works. We have shown the synthesis, in a micrometric range from 10 mm to 30 mm, of mesoporous ZnO microcapsules with a thin and flexible shell. We investigate the polar feature of ZnO nanoparticles and their interfacial self-organization. Besides, we reveal that the electric charges carried by ZnO primary units play a crucial role in the stability of the droplets in the presence and in the absence of charged molecules. It also plays a key role throughout the assembly process from the creation of the colloidal ZnO nanoparticles to the microdroplets, and finally the microspheres. We report, for the first time, the selforganization of doped-ZnO liquid microdroplets in square arrays. We demonstrate that such a result discloses the polar aspect of ZnO microdroplets and corroborate a shift in the balance between the driving forces controlling the ZnO nanoparticles organization at the nanoscale. We have developed different models, in very good agreement with the dipole-field and interfacial forces mechanisms, to support the experimental results put forward, and to explain the ZnO/RhB nanoparticles interfacial organization based on ZnO droplets organization properties. Based on our findings, and on the stated dependence of the microcapsules size, shell thickness, and nanoparticles surface density versus the droplets size, we provide an original model for the contribution of the involved factors in the shell formation mechanism
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Book chapters on the topic "Droplet based microfluidic"

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Sharma, Sanjiv, Monpichar Srisa-Art, Steven Scott, Amit Asthana, and Anthony Cass. "Droplet-Based Microfluidics." In Microfluidic Diagnostics, 207–30. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-134-9_15.

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Srisa-Art, Monpichar, and Sanjiv Sharma. "Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET." In Microfluidic Diagnostics, 231–40. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-134-9_16.

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Ozdemir, Pinar, and Yonghao Zhang. "DNA Analysis in Droplet-Based Microfluidic Devices." In Molecular Analysis and Genome Discovery, 56–80. Chichester, UK: John Wiley & Sons, Ltd, 2011. http://dx.doi.org/10.1002/9781119977438.ch4.

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Wang, Junming, and Jin-Ming Lin. "Droplet-Based Microfluidic Technology for Cell Analysis." In Cell Analysis on Microfluidics, 225–62. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5394-8_7.

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Pop, Paul, Mirela Alistar, Elena Stuart, and Jan Madsen. "Module-Based Compilation with Droplet-Aware Operation Execution." In Fault-Tolerant Digital Microfluidic Biochips, 105–14. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-23072-6_8.

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Haselmayr, Werner, Andrea Zanella, and Giacomo Morabito. "Communications and Networking in Droplet-Based Microfluidic Systems." In Encyclopedia of Wireless Networks, 210–16. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-319-78262-1_313.

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Haselmayr, Werner, Andrea Zanella, and Giacomo Morabito. "Communications and Networking in Droplet-Based Microfluidic Systems." In Encyclopedia of Wireless Networks, 1–7. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-319-32903-1_313-1.

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Cai, Qiuxian, and Chunxiong Luo. "Yeast Cell Electroporation in Droplet-Based Microfluidic Chip." In Fungal Biology, 211–16. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-10503-1_18.

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Du, Guan-Sheng, Jian-Zhang Pan, Shi-Ping Zhao, Ying Zhu, Jaap M. J. den Toonder, and Qun Fang. "A Microfluidic Droplet Array System for Cell-Based Drug Combination Screening." In Methods in Molecular Biology, 203–11. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7792-5_16.

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Su, F., S. Ozev, and K. Chakrabarty. "Test Planning and Test Resource Optimization for Droplet-Based Microfluidic Systems." In Emerging Nanotechnologies, 267–86. Boston, MA: Springer US, 2008. http://dx.doi.org/10.1007/978-0-387-74747-7_10.

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Conference papers on the topic "Droplet based microfluidic"

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Nguyen, Nam-Trung. "Thermal Control for Droplet-Based Microfluidics." In 2008 Second International Conference on Integration and Commercialization of Micro and Nanosystems. ASMEDC, 2008. http://dx.doi.org/10.1115/micronano2008-70277.

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This paper presents our recent works on thermal control for droplet-based microfluidics. Temperature dependent properties of liquids have been use for actuation and many other applications in droplet-based microfluidics. In analogy to an analog/digital electronic circuits, a droplet-based microfluidic system consists for three main subsystems: droplet formation (analog/digital converter), droplet manipulation (digital processing) and droplet merging (digital/analog converter). This paper will present our recent achievements in thermal control of droplet formation in different configurations such as T-junction and cross junction with integrated microheaters. Furthermore, results on droplet switching will be presented. The droplet switch represent basic logic gate that can be used to construct a more complex droplet-based digital network. Thermocapillary actuation of microdroplets in one-dimensional and two-dimensional microfluidic platforms will be presented. Both numerical and experimental results will be presented in this paper.
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Chokkalingam, Venkatachalam, Boris Weidenhof, Wilhelm F. Maier, Stephan Herminghaus, and Ralf Seemann. "Controlled Production of Monodispersed Silica Microspheres Using a Double Step-Emulsification Device." In ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2008. http://dx.doi.org/10.1115/icnmm2008-62109.

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We explore droplet based microfluidics to perform chemical reactions within microfluidic channels. By dispensing the different chemicals in droplets and subsequently merging the droplets containing different chemicals, the reactive mixture never gets in contact with the walls of the surrounding microfluidic channel. Using this approach we can realize chemical reactions for gels or precipitates, which are neither possible in single phase microfluidics, nor in droplet based microfluidics if the chemicals are mixed prior to dispersing the droplets. We explore this explicitly for the production of porous silica particles from sol-gel chemistry. All processing steps ranging from droplet production, synchronization of the droplets containing the different chemicals, combining the droplets, mixing and post processing are discussed and optimized for the particular demands.
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Li, Z. G., C. D. Ohl, K. Ando, J. B. Zhang, and A. Q. Liu. "Bubble-based droplet mixers microfluidic systems." In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2011. http://dx.doi.org/10.1109/memsys.2011.5734628.

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Hun Lee, Linfeng Xu, and Kwang W. Oh. "A journey of trains of droplets in droplet-based microfluidic devices." In 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2014. http://dx.doi.org/10.1109/embc.2014.6943706.

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Melbye, Julie A., and Yechun Wang. "Droplet Dynamics in Constricted Return Bends of Microfluidic Channels." In ASME 2020 Fluids Engineering Division Summer Meeting collocated with the ASME 2020 Heat Transfer Summer Conference and the ASME 2020 18th International Conference on Nanochannels, Microchannels, and Minichannels. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/fedsm2020-20406.

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Abstract Microfluidic delivery systems have been employed to facilitate cell seeding procedures in drug development for personalized medicine for cancer patients. Despite of the high-throughput nature and potential impact on clinical outcomes of these systems, the efficiency in cell trapping remains a challenge in the operation. Droplet-based microfluidics became one of the solutions due to the large size of the cell-enclosing droplets and their interfacial properties. This study is focused on the motion of the cell-enclosing droplet in a constricted return bends that help to restrict the release of the cells while maintaining the high-throughput nature of the device. In this preliminary study, a three-dimensional boundary element method is used to predict droplet shape, deformation and migration velocity under the influence of various fluid properties and operational conditions. A variety of channel geometries have been explored as well. The resulting computational framework will be used to guide the design of a droplet-based microfluidic delivery system for cell seeding in 3D tumor spheroid arrays.
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Recht, Michael I., Jacob Chamoun, Ashish Pattekar, and Joerg Martini. "High-throughput droplet-based microfluidic optical calorimeter." In Frontiers in Biological Detection: From Nanosensors to Systems XI, edited by Benjamin L. Miller, Sharon M. Weiss, and Amos Danielli. SPIE, 2019. http://dx.doi.org/10.1117/12.2511764.

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Hamidović, Medina, Uli Marta, Gerold Fink, Robert Wille, Andreas Springer, and Werner Haselmayr. "Information Encoding in Droplet-Based Microfluidic Systems." In NANOCOM '19: The Sixth Annual ACM International Conference on Nanoscale Computing and Communication. New York, NY, USA: ACM, 2019. http://dx.doi.org/10.1145/3345312.3345482.

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Zec, Helena, Tushar D. Rane, Wen-Chy Chu, and Tza-Huei Wang. "Microfluidic Combinatorial Screening Platform." In ASME 2012 10th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2012 Heat Transfer Summer Conference and the ASME 2012 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/icnmm2012-73159.

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We propose a microfluidic droplet-based platform that accepts an unlimited number of sample plugs from a multi-well plate, performs splitting of these sample droplets into smaller daughter droplets and subsequent synchronization-free, reliable fusion of sample daughter droplets with multiple reagents simultaneously. This system consists of two components: 1) a custom autosampler which generates a linear array of sub-microliter plugs in a microcapillary from a multi-well plate and 2) A microfluidic chip with channels for sample plug introduction, reagent merging and droplet incubation. This novel system generates large arrays of heterogeneous droplets from hundreds to thousands of samples while concurrently screening these arrays against a large array of reagents. This high throughput system minimizes sample and reagent consumption and can be applied to a gamut of biological assays, ranging from SNP detection to forensic screening.
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Motosuke, Masahiro, Asami Hoshi, and Shinji Honami. "Photothermal Marangoni Convection for the Usage of Characterized Droplet Manipulation in Microfluidic Chip." In ASME 2012 10th International Conference on Nanochannels, Microchannels, and Minichannels collocated with the ASME 2012 Heat Transfer Summer Conference and the ASME 2012 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/icnmm2012-73304.

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Droplet-based microfluidics which involves discrete volumes with the use of immiscible phases enable controlled and rapid mixing inside the droplet and promoted reaction of reagents or cells. It can be operated as “digital fluidic platform.” Due to high surface area to volume ratio of transport phenomena in microscale, an interfacial behavior becomes more predominant than continuous-flow-based microfluidics. In this study, we have investigated an interfacial flow control based on local photothermal excitation of the interfacial tension gradient resulting in Marangoni convection for droplet manipulation in a microfluidic chip. The surface Marangoni flow occurs by the local thermal gradient induced by the localized light irradiation which is spatially characterized by a mask with a specific aperture geometry. In controlled droplet generation and manipulation, oil-in-water (O/W) system, oleic acid as the dispersed phase, were used in the present experiments. Droplets have volumes from 0.5 to 65 pL, corresponding to diameters from 10 to 50 μm. A microfluidic chip consists of two PDMS (polydimethylsiloxiane) channel layers fabricated using the softlithography. Spatially characterized heating is produced by a DPSS laser with a wavelength of 532 nanometers, a mask with aperture and a reduced-projection exposure optics. The light irradiation generates local temperature change in the continuous phase which can cause interfacial tension gradient when droplets come to the illuminated area. As a result, the droplet experiences a repulsion force from the illuminated area with high temperature because the liquid-liquid interface in this case has positive temperature dependence on the tension. The droplet can be trapped in the microchannel when U- or V-shaped light pattern is irradiated. When a light pattern with nozzle-like geometry is irradiated, droplets were focused toward the exit of the nozzle avoiding the irradiated area. The performances of the trapping and focusing of droplets due to the optically-induced interfacial flow were evaluated through behaviors of droplets with different sizes and light powers. The estimation of forces acting on a drop due to the photothermal Marangoni convection was also conducted.
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Gopalan, Preethi, Byungwook Ahn, and Kwang W. Oh. "Serial Microfluidic Device for Micro Droplet Trapping and Pairing." In ASME 2010 International Mechanical Engineering Congress and Exposition. ASMEDC, 2010. http://dx.doi.org/10.1115/imece2010-38823.

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We propose a serial flowing microfluidic system for droplet generation along with a sequential trapping and pairing of micro-droplets. This device consists of three different functional regions: a flow focusing droplet generator; a single droplet trap region; and a pairing region. Our design is based on the principle of exploiting hydrodynamic resistance of the columnar structure in the microfluidic channel. By adjusting the flow rate and the fluid pressure inside the trapping area, the droplet trapping was precisely executed. The proposed method would continuously trap the droplets in the trapping area and when the reverse flow is applied, the droplet would be released and would enter the pairing chamber where it would be held until another droplet of different liquid to combine with it. Second droplet travels in the reverse flow direction and would be trapped in the pairing chamber to combine with first droplet.
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Reports on the topic "Droplet based microfluidic"

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Liaw, Steven. Develop Multiple Applications using Ultra-High Throughput Droplet-based Microfluidic Platform. Office of Scientific and Technical Information (OSTI), July 2014. http://dx.doi.org/10.2172/1143962.

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Liaw, Steven. Droplet Based Microfluidics. Office of Scientific and Technical Information (OSTI), July 2014. http://dx.doi.org/10.2172/1148311.

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