Relevant bibliographies by topics / Droplet digital PCR

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Droplet digital PCR'

Author: Grafiati
Published: 4 June 2021
Last updated: 10 March 2023

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Journal articles on the topic "Droplet digital PCR"

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Zhou, Wuping, Cong Liu, Tao Zhang, Keming Jiang, Haiwen Li, Zhiqiang Zhang, and Yuguo Tang. "Low Cost, Easily-Assembled Centrifugal Buoyancy-Based Emulsification and Digital PCR." Micromachines 13, no. 2 (January 24, 2022): 171. http://dx.doi.org/10.3390/mi13020171.

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Microfluidic-based droplet generation approaches require the design of microfluidic chips and a precise lithography process, which require skilled technicians and a long manufacturing time. Here we developed a centrifugal buoyancy-based emulsification (CBbE) method for producing droplets with high efficiency and minimal fabrication time. Our approach is to fabricate a droplet generation module that can be easily assembled using syringe needles and PCR tubes. With this module and a common centrifuge, high-throughput droplet generation with controllable droplet size could be realized in a few minutes. Experiments showed that the droplet diameter depended mainly on centrifugal speed, and droplets with controllable diameter from 206 to 158 μm could be generated under a centrifugal acceleration range from 14 to 171.9 g. Excellent droplet uniformity was achieved (CV < 3%) when centrifugal acceleration was greater than 108 g. We performed digital PCR tests through the CBbE approach and demonstrated that this cost-effective method not only eliminates the usage of complex microfluidic devices and control systems but also greatly suppresses the loss of materials and cross-contamination. CBbE-enabled droplet generation combines both easiness and robustness, and breaks the technical challenges by using conventional lab equipment and supplies.
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Schuler, Friedrich, Martin Trotter, Marcel Geltman, Frank Schwemmer, Simon Wadle, Elena Domínguez-Garrido, María López, et al. "Digital droplet PCR on disk." Lab on a Chip 16, no. 1 (2016): 208–16. http://dx.doi.org/10.1039/c5lc01068c.

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Fitarelli-Kiehl, Mariana, Fangyan Yu, Ravina Ashtaputre, Ka Wai Leong, Ioannis Ladas, Julianna Supplee, Cloud Paweletz, et al. "Denaturation-Enhanced Droplet Digital PCR for Liquid Biopsies." Clinical Chemistry 64, no. 12 (December 1, 2018): 1762–71. http://dx.doi.org/10.1373/clinchem.2018.293845.

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Abstract BACKGROUND Although interest in droplet-digital PCR technology (ddPCR) for cell-free circulating DNA (cfDNA) analysis is burgeoning, the technology is compromised by subsampling errors and the few clinical targets that can be analyzed from limited input DNA. The paucity of starting material acts as a “glass ceiling” in liquid biopsies because, irrespective how analytically sensitive ddPCR techniques are, detection limits cannot be improved past DNA input limitations. METHODS We applied denaturation-enhanced ddPCR (dddPCR) using fragmented genomic DNA (gDNA) with defined mutations. We then tested dddPCR on cfDNA from volunteers and patients with cancer for commonly-used mutations. gDNA and cfDNA were tested with and without end repair before denaturation and digital PCR. RESULTS By applying complete denaturation of double-stranded DNA before ddPCR droplet formation the number of positive droplets increased. dddPCR using gDNA resulted in a 1.9–2.0-fold increase in data-positive droplets, whereas dddPCR applied on highly-fragmented cfDNA resulted in a 1.6–1.7-fold increase. End repair of cfDNA before denaturation enabled cfDNA to display a 1.9–2.0-fold increase in data-positive signals, similar to gDNA. Doubling of data-positive droplets doubled the number of potential ddPCR assays that could be conducted from a given DNA input and improved ddPCR precision for cfDNA mutation detection. CONCLUSIONS dddPCR is a simple and useful modification in ddPCR that enables extraction of more information from low-input clinical samples with minor change in protocols. It should be applicable to all ddPCR platforms for mutation detection and, potentially, for gene copy-number analysis in cancer and prenatal screening.
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Meng, Xiangkai, Yuanhua Yu, and Guangyong Jin. "Numerical Simulation and Experimental Verification of Droplet Generation in Microfluidic Digital PCR Chip." Micromachines 12, no. 4 (April 7, 2021): 409. http://dx.doi.org/10.3390/mi12040409.

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The generation of droplets is one of the most critical steps in the droplet digital polymerase chain reaction (ddPCR) procedure. In this study, the mechanism of droplet formation in microchannel structure and factors affecting droplet formation were studied. The physical field of laminar two-phase flow level was used to simulate the process of droplet generation through microfluidic technology. The effect of the parameters including flow rate, surface tension, and viscosity on the generated droplet size were evaluated by the simulation. After that, the microfluidic chip that has the same dimension as the simulation was then, fabricated and evaluated. The chip was made by conventional SU-8 photolithography and injection molding. The accuracy of the simulation was validated by comparing the generated droplets in the real scenario with the simulation result. The relative error (RE) between experimentally measured droplet diameter and simulation results under different flow rate, viscosity, surface tension and contact angle was found less than 3.5%, 1.8%, 1.4%, and 1.2%, respectively. Besides, the coefficient of variation (CV) of the droplet diameter was less than 1%, which indicates the experimental droplet generation was of high stability and reliability. This study provides not only fundamental information for the design and experiment of droplet generation by microfluidic technology but also a reliable and efficient investigation method in the ddPCR field.
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Nugroho, Kristianto, Dwi Widyajayantie, Sayyidah Afridatul Ishthifaiyyah, and Elisa Apriliani. "Pemanfaatan Teknologi Droplet Digital PCR (ddPCR) dalam Kegiatan Analisis Molekuler Tanaman." JURNAL BIOS LOGOS 11, no. 1 (January 19, 2021): 28. http://dx.doi.org/10.35799/jbl.11.1.2021.31101.

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(Article History: Received 23 October 2020; Revised 9 January 2021; Accepted 18 January 2021) ABSTRAKSelama beberapa dekade terakhir, teknik PCR memberikan manfaat yang begitu besar dalam kegiatan penelitian di bidang biologi molekuler. Digital droplet PCR (ddPCR) merupakan salah satu teknologi PCR terbaru yang diklaim memiliki keunggulan dibanding teknik qPCR. Prinsip kerja teknik ini yaitu membagi sampel menjadi molekul-molekul kecil yang dipisahkan oleh emulsi minyak, air, dan senyawa penstabil sehingga membentuk droplets. Teknik ini memiliki kelebihan mampu melakukan kuantifikasi absolut maupun relatif pada DNA dengan konsentrasi sangat rendah, tidak memerlukan kurva standar, serta tidak sensitif terhadap kehadiran senyawa inhibitor. Teknik ini telah diaplikasikan pada kegiatan analisis molekuler tanaman di antaranya kegiatan pengukuran konsentrasi DNA dengan sangat akurat, deteksi kehadiran patogen pada jaringan tanaman, dan estimasi jumlah salinan T-DNA pada proses transformasi genetik.Kata kunci: PCR; droplet digital PCR; DNA; biologi molekuler; alat deteksi ABSTRACTOver the past decades, PCR technique has provided enormous benefits in molecular biology research activities. Digital droplet PCR (ddPCR) is one of the latest PCR technologies that is claimed to have advantages over the qPCR technique. The working principle of this technique is to divide the sample into small molecules, which separated by emulsions of oil, water, and stabilizing compounds to form droplets. This technique has the advantage of being able to perform absolute and relative quantification with very low DNA concentrations, does not require a standard curve, and less sensitive to the presence of inhibitor compounds. This technique has been applied to a number of plant molecular analysis, such as for measuring DNA concentrations very accurately, detecting the presence of pathogens in plant tissue, and estimating the copy number of T-DNA in the genetic transformation process.Keywords: PCR; droplet digital PCR; DNA; molecular biology; diagnostic tool.
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Rausch, Christian, Maja Rothenberg-Thurley, Simon A. Buerger, Sebastian Tschuri, Annika Dufour, Michaela Neusser, Stephanie Schneider, Karsten Spiekermann, Klaus H. Metzeler, and Frank Ziemann. "Double Drop-Off Droplet Digital PCR." Journal of Molecular Diagnostics 23, no. 8 (August 2021): 975–85. http://dx.doi.org/10.1016/j.jmoldx.2021.05.001.

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Lo Schirico, Mariella, Martina Ferrante, Irene Dogliotti, Alberto Zamò, Bruno Ferrero, Davide Bertuzzo, Giulia Benevolo, et al. "Droplet Digital PCR Assay for MYD88L265P." HemaSphere 4, no. 1 (February 2020): e324. http://dx.doi.org/10.1097/hs9.0000000000000324.

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Moser, Dirk A., Luca Braga, Andrea Raso, Serena Zacchigna, Mauro Giacca, and Perikles Simon. "Transgene Detection by Digital Droplet PCR." PLoS ONE 9, no. 11 (November 6, 2014): e111781. http://dx.doi.org/10.1371/journal.pone.0111781.

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Ragni, Margaret V. "Prenatal diagnosis by droplet digital PCR." Blood 130, no. 3 (July 20, 2017): 240–41. http://dx.doi.org/10.1182/blood-2017-05-786269.

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Dutra, Lara, Ole Franz, Veli-Mikko Puupponen, and Marja Tiirola. "DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen." BioTechniques 69, no. 6 (December 2020): 450–54. http://dx.doi.org/10.2144/btn-2020-0076.

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Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a simple and environmentally friendly protocol for recovering DNA from ddPCR.
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Dissertations / Theses on the topic "Droplet digital PCR"

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Attali, Dean. "Automatic analysis of dual-channel Droplet Digital PCR experiments." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/57928.

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The ability to quantify the amount of DNA in a sample is an essential technique in biology and is used in many fields of research. Droplet digital polymerase chain reaction (ddPCR) is an advanced technology developed for this purpose that enables more accurate and sensitive quantification than traditional real-time PCR. In ddPCR, nucleic acid (e.g., genomic DNA) within a sample is partitioned into thousands of droplets, along with the reagents needed to amplify and detect one or more DNA target sequences. After amplification takes place in all droplets, each droplet is individually read by a two-colour fluorescence detection system to determine whether or not it contains the target sequence. ddPCR experiments utilizing both fluorescence wavelengths are termed dual-channel, while simpler experiments can make use of only one fluorescence wavelength and are thus classified as single-channel. Droplets containing amplified product exhibit high fluorescence and are said to be positive, while those without product show little or no fluorescence and are considered negative. Using this binary, or digital, classification of droplets, the number of positive and negative droplets can be counted to allow for an absolute quantification of template abundance in the starting sample. ddPCR instruments are now available commercially and their use is growing. But, there are a very limited number of tools available for downstream data analysis. The key step in ddPCR data analysis is droplet gating: using the end-point fluorescence data to gate, or classify, droplets as either positive or negative for a given template. The proprietary software provided by BioRad Inc., a ddPCR instrument manufacturer, is currently the only program available to automatically analyze dual-channel ddPCR data. However, because this analysis tool often produces poor results, many ddPCR users resort to time-consuming and subjective manual data analyses, emphasizing the clear need for new ddPCR analysis tools. In this thesis, I devise an algorithm for automatic analysis of dual-channel ddPCR data that can objectively and reproducibly perform droplet gating. The proposed analysis method has been implemented in an R package and is also available as a web application online for easy and open access to any ddPCR user.
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Jakobsson, Sanna. "Quantitative analysis of BCR-ABL1 fusion gene by Droplet Digital PCR and qRT-PCR." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-103957.

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Pettersson, Fredrika. "Identifiering och kvantifiering av humant papillomvirus typ 16 med droplet digital PCR." Thesis, Örebro universitet, Institutionen för hälsovetenskap och medicin, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-44983.

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Gautier, Célia. "Variation de l’expression génique au cours de l’hibernation du hamster d’Europe : un rôle des récepteurs à la mélatonine ?" Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAJ011/document.

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Afin de faire face aux conditions environnementales défavorables, certains animaux réduisent drastiquement leur activité métabolique et leur température grâce à des phases de torpeur hivernal. L’objectif de cette étude est d’établir une signature moléculaire de chacune des phases d’hibernation. Pour cela, les variations d’expression de 21 gènes impliqués dans le contrôle des fonctions saisonnières (horloge circadienne, hormones thyroïdiennes, récepteurs à la mélatonine) et le métabolisme ont été étudiées dans 8 organes. Les résultats ont mis en évidence une augmentation ubiquitaire de l’expression des gènes Périodes indiquant un possible réajustement de l’horloge au début de la phase de réveil. Ainsi qu’une régulation spécifique des déiodinases induisant une augmentation de la synthèse de thyroxine dans le tissu adipeux brun et l’hypothalamus pendant la torpeur et le réveil. Le récepteur MT2 du hamster d’Europe a été partiellement caractérisé génétiquement et pharmacologiquement. A la différence d’autres espèces de hamster dont le récepteur MT2 est tronqué, le récepteur étudié semble être fonctionnel pour la mélatonine et pourrait être critique durant l’hibernation
Living in the wild involves to cope with a variable seasonal environment availability. When winter is coming, animals use various strategies to adapt to hostile environment by limiting energy expenditure such as hibernation. In this study, expression of 21 selected genes was compared at different states of the hibernation cycle of the true hibernator European hamster. Level of mRNA encoding proteins involved in seasonal timing (melatonin receptors, thyroid metabolism, clock) and energy homeostasis were measured by digital droplet PCR in eight central and peripheral organs. During the arousal phase, Periods genes expression is increased in all organs indicating a possible resetting of body’s clocks at the beginning of the active period. The brown adipose tissue displays a specific regulation of deiodinases leading to increased synthesis of thyroxine during both torpor and arousal. The melatonin receptor MT2 of the European hamster had been partially cloned and pharmacologically characterized. While in most hamster species, MT2 is a natural knock out, the studied receptor seems to be functional and could be critical during hibernation
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Brugière, Charlotte. "L'invasion péri-nerveuse des carcinomes épidermoïdes cutanés humains." Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC193.

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Le carcinome épidermoïde cutané (CEC) représente un enjeu important par sa fréquence et sa gravité potentielle.L’agressivité de ce cancer est liée à l’invasion péri-nerveuse (IPN), mode d’envahissement tumoral reconnu comme un facteur de mauvais pronostic.L’objectif de ce travail est de s’intéresser aux mécanismes favorisant l’IPN, en comparant 2 groupes appariés de CEC humains, avec et sans IPN.Pour cela nous avons réalisé une étude de facteurs et récepteurs neurotrophiques, de marqueurs de la transition épithélio-mésenchymateuse (TEM), et de la molécule NCAM1, par analyse immunohistochimique à partir de pièces chirurgicales de CEC et par analyse moléculaire en droplet digital PCR sur des cellules tumorales microdisséquées.L’analyse immunohistochimique a trouvé une forte expression de BDNF, TrkB, p75NGFR, Snail 1 et NCMA1 dans les cellules tumorales péri-nerveuses, contrastant avec une faible expression de ces marqueurs dans les cellules tumorales à distance du nerf. L’E-cadhérine était diminuée dans les cellules tumorales péri-nerveuses.L’analyse moléculaire en ddPCR montrait une diminution d’expression de l’E-cadhérine et une surexpression de BDNF, TrkB, p75NGFR, Snail1, Slug, Zeb2, Twist1 et NCAM1 dans les cellules tumorales péri-nerveuses par rapport aux cellules tumorales distantes du nerf.Nous avons démontré dans ce travail que l’invasion péri-nerveuse dans les CEC humains est liée aux neurotrophines, à la TEM et implique NCAM1
Cutaneous squamous cell carcinoma (SCC) is an important issue because of its frequency and potential severity.The aggressiveness of this cancer is related to perineural invasion (PNI), a mode of tumor dissemination recognized as a poor prognosis factor.The aim of this work is to study the mechanisms of PNI, comparing 2 matched- groups of human SCC with and without PNI.For this, we studied neurotrophins, epithelial-mesenchymal transition (EMT) markers, and the NCAM1 molecule, by immunohistochemistry analysis on surgical pieces of SCC and by molecular analysis with digital-droplet PCR on laser-microdissected tumor cells.Immunohistochemistry analysis found strong expression of BDNF, TrkB, p75NGFR, Snail 1 and NCMA1 in perineural tumor cells, contrasting with weak expression of these markers in tumor cells distant from the nerves. E-cadherin was decreased in perineural tumor cells.Molecular analysis in ddPCR showed decreased expression for E-cadherin and overexpression of BDNF, TrkB, p75NGFR, Snail1, Slug, Zeb2, Twist1 and NCAM1 in perineural tumor cells compared to tumor cells distant from the nerves.We have demonstrated in this work that PNI in human SCC is linked to neurotrophins and EMT, and involves NCAM1
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Pekin, Deniz. "Development of novel droplet-based microfluidic strategies for the molecular diagnosis of cancer." Phd thesis, Université de Strasbourg, 2013. http://tel.archives-ouvertes.fr/tel-00856594.

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The aim of this work is to establish novel strategies for the highly sensitive screening of cancer biomarkers in biological samples.To achieve this goal, we developed droplet-based microfluidic dPCR technique. Using a limiting dilution, individual DNA molecules are encapsulated within monodisperse droplets of a water-in-oil emulsion created with a microfluidic device. Fluorescent TaqMan® probes targeting the screened cancer biomarkers allow the detection of mutations. We focused on the mutations in the human KRAS gene for the development of our test. This method is also transposable in a multiplexed format for the parallel detection of multiple mutations in clinical samples.The developed technique allowed the precise quantification of a mutated KRAS gene in the presence of a 200,000-fold excess of un-mutated KRAS genes and enabled the determination of mutant allelic specific imbalance (MASI) in several cancer cell-lines. We validated our technique by screening for KRAS mutations in the blood plasma and tumor samples from patients with metastatic colorectal cancer.
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Wågberg, Johanna. "Detection of hotspot mutations in IDH1/2 in patients withacute myeloid leukemia using Droplet Digital PCR." Thesis, Örebro universitet, Institutionen för medicinska vetenskaper, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-81559.

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IntroductionAcute myeloid leukemia (AML) is caused by a wide range of genetic aberrations, includingmutations within the genes that encode the enzymes isocitrate dehydrogenase 1 and 2(IDH1/2). Drugs that target mutant IDH1/2 are now available, which makes assessment of themutational status of IDH1/2 important in clinical diagnostics of AML. A promising method todetect these mutations is the droplet digital polymerase chain reaction (ddPCR), which showsadvantages of a high sensitivity and a simple workflow.AimTo evaluate ddPCR as method of choice to detect hotspot mutations in IDH1 (codon R132)and IDH2 (codon R140 and R172) in patients with AML.MethodsFifteen AML patients known to be positive for IDH1/2 diagnosed by a previously performednext generation sequencing (NGS) were selected for evaluation of ddPCR. Diagnosticsamples were tested for 14 patients, whereas follow-up samples were tested for one patient.ddPCR was performed using QX200™ Droplet Digital PCR system and data were presentedas fractional abundance of mutant allele.ResultsThe amount of mutant IDH1/2 in samples reported by ddPCR correlated well with the resultsfrom NGS when using probes that target their specific mutation. The detection limit formutant allele in the background of wild type IDH1/2 was 0,5% for IDH2 p.R140Q and 0.1%for IDH1 p.R132C/H.ConclusionddPCR that target specific mutations shows a great potential in measuring minimal residualdisease during follow-up. However, its use in screening for mutant IDH1/2 at the time ofdiagnosis is limited and alternative approaches should be considered.
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Ziller, Antoine. "Origine(s) et Fonction(s) de Gènes de Résistance aux Métaux Issus de Métatranscriptomes Eucaryotes de Sols." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSE1056/document.

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Le sol est essentiel à toute société humaine notamment pour la production d'aliments. Son fonctionnement repose sur des réseaux d'interactions entre les éléments qui le composent et toute perturbation modifie ces réseaux. Les microorganismes eucaryotes représentent une composante importante de l'écosystème édaphique car ils sont impliqués dans des processus essentiels comme la régulation de populations de procaryotes. Mais paradoxalement, ils restent peu étudiés comparés aux bactéries notamment lorsqu'on s'intéresse aux cycles biogéochimiques autres que celui du carbone comme par exemple ceux des métaux. Suite à une pollution par des métaux, certains de ces microorganismes eucaryotes développent des mécanismes « de résistance » cellulaires. Dans ce contexte, le laboratoire d'accueil a isolé, directement à partir d'extraits d'ARN de sol, des gènes eucaryotes impliqués dans la résistance cellulaire au Cd. Ces nouveaux gènes forment une famille codant des protéines riches en cystéines dont les positions sont conservées au sein de cette famille. Mon projet de thèse a eu pour but de caractériser l'origine taxonomique et la fonction de cette famille de gènes. Dans un premier temps, la purification de cinq de ces protéines produites dans Escherichia coli et leurs caractérisations biochimiques par des méthodes spectrométriques ont permis de montrer que cette famille génique constitue une nouvelle famille de métallothionéines capables de chélater in vitro le Zn, le Cu et le Cd. Dans un second temps, une méthode de quantification par PCR quantitative de l'expression de ces gènes, extraits à partir de sol provenant de microcosmes, a été mise au point. Dans un troisième temps, nous avons tenté d'obtenir les régions génomiques bordant ces gènes environnementaux afin d'affilier les organismes qui les portent à un groupe taxonomique et d'analyser les régions promotrices de ces gènes par capture ciblée de gènes
Soil is essential to human societies, especially for food production. Its functioning relies on interaction networks sensitive to environmental alterations. Eukaryotic microorganisms are an important component of the soil ecosystem where they are involved in essential processes such as the regulation of prokaryotic populations. However, they remain poorly studied compared to bacteria, especially concerning their roles in biogeochemical cycles other than the carbon one such as metal cycles. In response to soil metal contamination, some of these eukaryotic microorganisms develop cellular "resistance" mechanisms. In this context, the host laboratory has previously isolated, directly from soils, eukaryotic genes able to confer Cd resistance. These genes form a family coding for cysteine-rich proteins whose cysteine positions are conserved within this sequences. My thesis project aimed at characterizing the function and taxonomic origin of this gene family. First, the purification of five of these proteins produced in Escherichia coli and their biochemical characterizations by spectrometric methods demonstrated that this gene family constitutes a new family of metallothioneins capable of chelating in vitro Zn, Cu and Cd. Some of these proteins are also able to confer Zn resistance when expressed in a sensitive yeast strain. In a second step, quantitative PCR methods for measuring expression levels of these genes in soil microcosms were developed. This will allow to evaluate the level of expression of these genes as a function of an increasing supply of exogenous metal. In a third step, we tried to obtain the genomic regions flanking these environmental genes in order to be able to associate the organisms from which they originate to a taxonomic group and to analyze the promoter regions of these genes using targeted capture
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Benning, Louise [Verfasser], Ingo [Akademischer Betreuer] Ahrens, and Marcus Sebastian [Akademischer Betreuer] Hortmann. "Droplet Digital PCR zur Quantifizierung von miR-21, miR-208a und miR-499 in der Diagnostik von Patienten mit funktionell relevanter koronarer Herzkrankheit." Freiburg : Universität, 2020. http://d-nb.info/1208623907/34.

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Bartolo, Jean-François. "Développement de sondes et de systèmes microfluidiques pour la détection de nouveaux biomarqueurs spécifiques." Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAF052/document.

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L’efficacité des traitements contre diverses pathologies dépend dans bien des cas de la précocité de la prise en charge des patients. Ce contexte pousse de nos jours les chercheurs à élaborer de nouvelles méthodes de diagnostic, généralement basées sur la détection de biomarqueurs spécifiques, permettant d’établir une corrélation entre un dérèglement moléculaire de l’organisme et la survenue d’une maladie. L’objectif de ces travaux était, par l’utilisation de la microfluidique digitale en gouttelettes, d’établir de nouvelles procédures simples et reproductibles, témoignant d’une sensibilité importante afin de déterminer d’infimes variations de l’état moléculaire de l’organisme à travers la recherche de biomarqueurs spécifiques. Pour cela nous avons élaboré une nouvelle gamme de tensioactifs fluorées adaptés aux applications biologiques en microfluidique digitale, ainsi que différentes stratégies d’étude des variations de l’expression de microARN extrait d’échantillons biologiques, basées respectivement sur les réactions induites par hybridation nucléotidique et sur la réaction de RT-PCR digitale
Efficiency of treatments for various diseases depends in many cases in precocity of patient management. Nowadays, this context urges researchers to develop new methods of diagnosis, generally based on the detection of specific biomarkers. These new methods allowing to establish correlations between physiological disorders and arisen of diseases states.The aim of this study was, by the use of droplet-based microfluidic, to work out a simple and reproducible procedure, with an increased sensitivity, to determine tiny variations of physiological state through the detection of specific biomarkers. Thus, we developed a new range of fluorinated surfactants fitted to biological applications in droplet-based microfluidics as well as various strategies to study variations of microRNA expressions in a biological sample. These methods, based on DNA-template reaction and digital PCR reaction, allows performing a substantial number of simultaneous reactions in micro-compartments (microdroplets) of picolitre volumes
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Book chapters on the topic "Droplet digital PCR"

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Mehle, Nataša, and Tanja Dreo. "Quantitative Analysis with Droplet Digital PCR." In Phytoplasmas, 171–86. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8837-2_14.

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Yu, Ming, Tai J. Heinzerling, and William M. Grady. "DNA Methylation Analysis Using Droplet Digital PCR." In Methods in Molecular Biology, 363–83. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7778-9_21.

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Parkin, Brian. "Rare Variant Quantitation Using Droplet Digital PCR." In Methods in Molecular Biology, 239–51. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8876-1_18.

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Vossen, Rolf H. A. M., and Stefan J. White. "Quantitative DNA Analysis Using Droplet Digital PCR." In Methods in Molecular Biology, 167–77. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6442-0_11.

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Gegevicius, Emilis, Karolis Goda, and Linas Mazutis. "CHAPTER 4. Droplet Gene Analysis – Digital PCR." In Soft Matter Series, 89–121. Cambridge: Royal Society of Chemistry, 2020. http://dx.doi.org/10.1039/9781839162855-00089.

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Yan, Irene K., Rishabh Lohray, and Tushar Patel. "Droplet Digital PCR for Quantitation of Extracellular RNA." In Methods in Molecular Biology, 155–62. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7652-2_12.

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Ferracin, Manuela, and Massimo Negrini. "Quantification of Circulating MicroRNAs by Droplet Digital PCR." In Methods in Molecular Biology, 445–57. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7778-9_25.

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Bell, Avery Davis, Christina L. Usher, and Steven A. McCarroll. "Analyzing Copy Number Variation with Droplet Digital PCR." In Methods in Molecular Biology, 143–60. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7778-9_9.

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Härmälä, Suvi K., Robert Butcher, and Chrissy H. Roberts. "Copy Number Variation Analysis by Droplet Digital PCR." In Methods in Molecular Biology, 135–49. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7231-9_9.

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Gutiérrez-Aguirre, Ion, Nejc Rački, Tanja Dreo, and Maja Ravnikar. "Droplet Digital PCR for Absolute Quantification of Pathogens." In Plant Pathology, 331–47. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2620-6_24.

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Conference papers on the topic "Droplet digital PCR"

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Zhang, Jie, Jiaqi Yan, Jinxian Wang, Jie Xu, Yilong Zhao, and Gangyin Luo. "Research on Droplet Digital PCR Amplification System." In 2021 IEEE 20th International Conference on Trust, Security and Privacy in Computing and Communications (TrustCom). IEEE, 2021. http://dx.doi.org/10.1109/trustcom53373.2021.00212.

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Meng, Xiangkai, Guangyong Jin, Yuanhua Yu, Jian Li, and Xue Tao. "Design of integrated droplet digital PCR gene chip." In Global Intelligent Industry Conference 2020, edited by Liang Wang. SPIE, 2021. http://dx.doi.org/10.1117/12.2590032.

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Yang, Jiayi, Boqiang Fu, Chunyan Niu, and Jing Wang. "Droplet digital PCR for quantitative detection of Escherichia coli." In INTERNATIONAL SYMPOSIUM ON THE FRONTIERS OF BIOTECHNOLOGY AND BIOENGINEERING (FBB 2019). AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5110837.

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Meng, Xiangkai, Guangyong Jin, Yuan Si, and Xue Tao. "Study on autofluorescence characteristics of micro droplet digital PCR chip." In 2020 International Conference on Optoelectronic Materials and Devices, edited by Siting Chen and Pei Wang. SPIE, 2021. http://dx.doi.org/10.1117/12.2592171.

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Shelton, Dawne, Jack Regan, George Karlin-Neumann, Jue Lin, and Elizabeth Blackburn. "Abstract LB-253: TRAPing telomerase activity using droplet digital PCR." In Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1538-7445.am2013-lb-253.

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So, Austin, Benjamin Hindson, Ryan Koehler, Serge Saxonov, George Karlin-Neumann, Nolan Ericson, and Jason Bielas. "Abstract 3399: Detection of rare mutations in plasma by droplet digital PCR." In Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1538-7445.am2012-3399.

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Huang, Jen-Yu, Shu-Sheng Lee, and Yu-Hsiang Hsu. "Development of an imaging method for quantifying a large digital PCR droplet." In SPIE BiOS, edited by Gerard L. Coté. SPIE, 2017. http://dx.doi.org/10.1117/12.2251801.

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Hashida, Shinsuke, Kadoaki Ohashi, Takehiro Matsubara, Tomoaki Ohtsuka, Mototsugu Watanabe, Ken Suzawa, Yuho Maki, et al. "Abstract 5248: Non-invasive EGFR T790M detection using droplet digital PCR system." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-5248.

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Lee, Kyoungmin, Kazuko Sakai, Min-Hee Ryu, Jae-Joon Kim, Young Soo Park, Young-Soon Na, Jungeun Ma, Hana Na, Kazuto Nishio, and Yoon-Koo Kang. "Abstract 3986: Digital droplet PCR measurement for plasmaHER2amplification in patients with AGC." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-3986.

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Lee, Kyoungmin, Kazuko Sakai, Min-Hee Ryu, Jae-Joon Kim, Young Soo Park, Young-Soon Na, Jungeun Ma, Hana Na, Kazuto Nishio, and Yoon-Koo Kang. "Abstract 3986: Digital droplet PCR measurement for plasmaHER2amplification in patients with AGC." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-3986.

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