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Journal articles on the topic 'Droplet Recombinase Polymerase Amplification'

Author: Grafiati

Published: 6 September 2023

Last updated: 31 July 2025

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1

Schuler, Friedrich, Frank Schwemmer, Martin Trotter, et al. "Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA." Lab on a Chip 15, no. 13 (2015): 2759–66. http://dx.doi.org/10.1039/c5lc00291e.

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2

Cui, Johnson Q., Frank X. Liu, Hojeong Park, et al. "Droplet digital recombinase polymerase amplification (ddRPA) reaction unlocking via picoinjection." Biosensors and Bioelectronics 202 (April 2022): 114019. http://dx.doi.org/10.1016/j.bios.2022.114019.

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3

Ibekwe, Mark A., Shelton E. Murinda, Stanley Park, et al. "Comparative Use of Quantitative PCR (qPCR), Droplet Digital PCR (ddPCR), and Recombinase Polymerase Amplification (RPA) in the Detection of Shiga Toxin-Producing E. coli (STEC) in Environmental Samples." Water 12, no. 12 (2020): 3507. http://dx.doi.org/10.3390/w12123507.

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E. coli O157:H7 is a foodborne pathogen that constitutes a global threat to human health. However, the quantification of this pathogen in food and environmental samples may be problematic at the low cell numbers commonly encountered in environmental samples. In this study, we used recombinase polymerase amplification (RPA) for the detection of E. coli O157:H7, real-time quantitative PCR (qPCR) for quantification, and droplet digital PCR (ddPCR) for absolute and accurate quantification of E. coli O157:H7 from spiked and environmental samples. Primer and probe sets were used for the detection of

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4

Lin, Hsing-Ying, Chen-Han Huang, Peng-Wei Hsu, et al. "(Invited) Aiot-Integrated Digital Imaging Sensor for Molecular-Fingerprint Profiling of Extracellular Vesicles in Liquid Biopsy." ECS Meeting Abstracts MA2024-01, no. 33 (2024): 1595. http://dx.doi.org/10.1149/ma2024-01331595mtgabs.

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The advancement of liquid biopsy techniques for non-invasive tumor analysis holds immense potential in continuously tracking tumor status—recurrence, progression, and treatment response—in real-time. To comprehensively assess tumor evolution, cellular diversity, and drug resistance mechanisms, a pivotal step involves scrutinizing proteins and nucleic acids within extracellular vesicles (EVs) present in biofluids. Our focus lies in crafting an advanced digital bead-based sensor system that seamlessly evaluates the molecular profile of EVs in glioblastoma cases. Our innovative approach enriches

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Lin, Hsing-Ying, Chen-Han Huang, Peng-Wei Hsu, et al. "(Invited) liquid Biopsy Tool: Integrated Aiot Digital Bead-Based Sensor for Molecular-Fingerprint Profiling of Extracellular Vesicles." ECS Meeting Abstracts MA2023-01, no. 34 (2023): 1914. http://dx.doi.org/10.1149/ma2023-01341914mtgabs.

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Development of liquid biopsies for non-invasive tumor characterization techniques shows great promise for real-time monitoring of tumor recurrence, progression, and treatment response. To better assess the various tumor evolution, cellular heterogeneity, consequent drug-resistance mechanisms, it is critical to screen proteins and nucleic acids of extracellular vesicles (EVs) in biofluids. We are developing an integrated sensitive digital bead-based sensor enabling evaluation of molecular profiling of extracellular vesicles in glioblastoma. The tumor relevant genetic information in EVs is enric

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6

Chowdhury, Rajashree, Prakash Ghosh, Md Anik Ashfaq Khan, et al. "Evaluation of Rapid Extraction Methods Coupled with a Recombinase Polymerase Amplification Assay for Point-of-Need Diagnosis of Post-Kala-Azar Dermal Leishmaniasis." Tropical Medicine and Infectious Disease 5, no. 2 (2020): 95. http://dx.doi.org/10.3390/tropicalmed5020095.

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To detect Post-kala-azar leishmaniasis (PKDL) cases, several molecular methods with promising diagnostic efficacy have been developed that involve complicated and expensive DNA extraction methods, thus limiting their application in resource-poor settings. As an alternative, we evaluated two rapid DNA extraction methods and determined their impact on the detection of the parasite DNA using our newly developed recombinase polymerase amplification (RPA) assay. Skin samples were collected from suspected PKDL cases following their diagnosis through national guidelines. The extracted DNA from three

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7

Daher, Rana K., Gale Stewart, Maurice Boissinot, and Michel G. Bergeron. "Isothermal Recombinase Polymerase Amplification Assay Applied to the Detection of Group B Streptococci in Vaginal/Anal Samples." Clinical Chemistry 60, no. 4 (2014): 660–66. http://dx.doi.org/10.1373/clinchem.2013.213504.

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Abstract BACKGROUND Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification have advantages relative to PCR in terms of the speed of reaction and simplicity. METHODS We studied the clinical performance of recombinase polymerase amplification for the screening of group B streptococci in vaginal/anal samples from 50 pregnant women. We also compared

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8

Daher, Rana K., Gale Stewart, Maurice Boissinot, and Michel G. Bergeron. "Recombinase Polymerase Amplification for Diagnostic Applications." Clinical Chemistry 62, no. 7 (2016): 947–58. http://dx.doi.org/10.1373/clinchem.2015.245829.

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Abstract BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years. The widespread adoption of this isothermal molecular tool in many diagnostic fields represents an affordable (approximately 4.3 USD per test), simple (few and easy hands-on steps), fast (results within 5–20 min), and sensitive (single target copy number detected) method for the identification of pathogens and the detection of single nucleotide polymorphisms in human cancers and genetica

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9

Tomar, Saurabh, Barbora Lavickova, and Carlotta Guiducci. "Recombinase polymerase amplification in minimally buffered conditions." Biosensors and Bioelectronics 198 (February 2022): 113802. http://dx.doi.org/10.1016/j.bios.2021.113802.

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10

Khmeleva, S. A., G. R. Kutdusova, I. F. Duskaev, et al. "DEOXYURIDINE TRIPHOSPHATES MODIFIED WITH AROMATIC GROUPS OF TYROSINE OR TRYPTOPHAN FOR DIRECT ELECTROCHEMICAL DETERMINATION OF DOUBLE-STRANDED DNA AMPLIFICATION PRODUCTS." http://eng.biomos.ru/conference/articles.htm 1, no. 19 (2021): 248–50. http://dx.doi.org/10.37747/2312-640x-2021-19-248-250.

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A set of deoxyuridine triphosphates modified with aromatic groups of tyrosine or tryptophan was studied as substrates for amplification (by polymerase chain reaction or recombinase polymerase amplification) and as carriers of an electroactive ‘label’ for direct electrochemical detection of double-stranded DNA.

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11

Choi, Goro, Jae Hwan Jung, Byung Hyun Park, et al. "A centrifugal direct recombinase polymerase amplification (direct-RPA) microdevice for multiplex and real-time identification of food poisoning bacteria." Lab on a Chip 16, no. 12 (2016): 2309–16. http://dx.doi.org/10.1039/c6lc00329j.

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12

Shahrajabian, Mohamad H., Wenli Sun, and Qi Cheng. "Different Methods for Molecular and Rapid Detection of Human Novel Coronavirus." Current Pharmaceutical Design 27, no. 25 (2021): 2893–903. http://dx.doi.org/10.2174/1381612827666210604114411.

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Introduction: While PCR has been recognized as one of the appropriate ways to diagnose infectious diseases, Loop-mediated isothermal amplification (LAMP) which is a nucleic acid amplification method, can be considered as an alternative to PCR, and it is faster, cost-effective, and easier to perform than nested PCR. Patients and Methods: Keywords were searched in PubMed/MEDLINE, Scopus and Institute for Scientific Information Web of Science, as well as the search engine of Google Scholar. Keywords included PCR, LAMP, RAA, RPA, Virus and COVID-19. Results: LAMP technology has been extensively ap

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13

Lei, Rong, Zhengyue Yan, Fan Hu, Shuifang Zhu, Yufen Xiong, and Xiaohong Fan. "Rapid identification of quarantine invasiveSolanum elaeagnifoliumby real-time, isothermal recombinase polymerase amplification assay." RSC Advances 7, no. 83 (2017): 52573–80. http://dx.doi.org/10.1039/c7ra10781a.

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14

Wang, Ying, Xiangdong Li, Dongmei Xi, and Xiaoqiang Wang. "Visual detection of Fusarium proliferatum based on asymmetric recombinase polymerase amplification and hemin/G-quadruplex DNAzyme." RSC Advances 9, no. 64 (2019): 37144–47. http://dx.doi.org/10.1039/c9ra05709a.

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15

Nair, Gayatri, Juan David Ramírez, A. Clinton White, et al. "Detection of Entamoeba histolytica by Recombinase Polymerase Amplification." American Journal of Tropical Medicine and Hygiene 93, no. 3 (2015): 591–95. http://dx.doi.org/10.4269/ajtmh.15-0276.

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16

Higgins, Matthew, Matt Ravenhall, Daniel Ward, et al. "PrimedRPA: primer design for recombinase polymerase amplification assays." Bioinformatics 35, no. 4 (2018): 682–84. http://dx.doi.org/10.1093/bioinformatics/bty701.

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17

Lobato, Ivan Magriñá, and Ciara K. O'Sullivan. "Recombinase polymerase amplification: Basics, applications and recent advances." TrAC Trends in Analytical Chemistry 98 (January 2018): 19–35. http://dx.doi.org/10.1016/j.trac.2017.10.015.

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18

Wang, Jianchang, Jinfeng Wang, Libing Liu, Ruiwen Li, and Wanzhe Yuan. "Rapid detection ofPorcine circovirus 2by recombinase polymerase amplification." Journal of Veterinary Diagnostic Investigation 28, no. 5 (2016): 574–78. http://dx.doi.org/10.1177/1040638716654201.

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19

Kim, Jeongyong, Jin-Seok Jung, Sungkyeong Lee, and Jung-Yoon Yoo. "Applications of Recombinase Polymerase Amplification in Forensic Science." Biomedical Science Letters 31, no. 2 (2025): 99–114. https://doi.org/10.15616/bsl.2025.31.2.99.

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20

Liu, Dan, Haicong Shen, Yuqian Zhang, et al. "A microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay for rapid COVID-19 detection." Lab on a Chip 21, no. 10 (2021): 2019–26. http://dx.doi.org/10.1039/d0lc01222j.

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21

Wan Rasni, Wan Hawa Najibah, Nazariyah Yahaya, and Maryam Mohamed Rehan. "Recombinase Polymerase Amplification and Their Application in Phytopathogen Detection." Malaysian Journal of Science Health & Technology 8, no. 2 (2022): 14–24. http://dx.doi.org/10.33102/2022254.

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DNA identification method is indispensable for the detection of a plant pathogen. However, established techniques, though reliable, requires advanced equipment, and their application outside specialized laboratories is limited. Along with the advancement of molecular techniques, several isothermal amplification methods, including Recombinase Polymerase Amplification (RPA), has been developed in this study. In fact, RPA is a rapid and sensitive amplification method, operating optimally at 37-42 degree celcius for 15 to 30 minutes with minimal sample preparation, and can amplify as low as 1-10 t

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22

Wan Rasni, Wan Hawa Najibah, Nazariyah Yahaya, and Maryam Mohamed Rehan. "Recombinase Polymerase Amplification and Their Application in Phytopathogen Detection." Malaysian Journal of Science Health & Technology 8, no. 2 (2022): 14–24. http://dx.doi.org/10.33102/mjosht.v8i2.254.

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DNA identification method is indispensable for the detection of a plant pathogen. However, established techniques, though reliable, requires advanced equipment, and their application outside specialized laboratories is limited. Along with the advancement of molecular techniques, several isothermal amplification methods, including Recombinase Polymerase Amplification (RPA), has been developed in this study. In fact, RPA is a rapid and sensitive amplification method, operating optimally at 37-42 degree celcius for 15 to 30 minutes with minimal sample preparation, and can amplify as low as 1-10 t

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23

Eid, Charbel, and Juan G. Santiago. "Assay for Listeria monocytogenes cells in whole blood using isotachophoresis and recombinase polymerase amplification." Analyst 142, no. 1 (2017): 48–54. http://dx.doi.org/10.1039/c6an02119k.

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24

Behrmann, Ole, Iris Bachmann, Frank Hufert, and Gregory Dame. "Schnellnachweis von SARS-CoV-2 mit recombinase polymerase amplification." BIOspektrum 26, no. 6 (2020): 624–27. http://dx.doi.org/10.1007/s12268-020-1458-3.

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Abstract The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39–42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.

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25

Li, Jia, Joanne Macdonald, and Felix von Stetten. "Correction: Review: a comprehensive summary of a decade development of the recombinase polymerase amplification." Analyst 145, no. 5 (2020): 1950–60. http://dx.doi.org/10.1039/c9an90127b.

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26

Yang, Huan-Lan, Shuang Wei, Ravi Gooneratne, et al. "Development of a recombinase polymerase amplification assay forVibrio parahaemolyticusdetection with an internal amplification control." Canadian Journal of Microbiology 64, no. 4 (2018): 223–30. http://dx.doi.org/10.1139/cjm-2017-0504.

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A novel RPA–IAC assay using recombinase polymerase and an internal amplification control (IAC) for Vibrio parahaemolyticus detection was developed. Specific primers were designed based on the coding sequence for the toxR gene in V. parahaemolyticus. The recombinase polymerase amplification (RPA) reaction was conducted at a constant low temperature of 37 °C for 20 min. Assay specificity was validated by using 63 Vibrio strains and 10 non-Vibrio bacterial species. In addition, a competitive IAC was employed to avoid false-negative results, which co-amplified simultaneously with the target sequen

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27

Kutdusova, G., and E. Suprun. "DEOXYURIDINE TRIPHOSPHATES MODIFIED WITH AROMATIC TYROSINE GROUPS FOR ELECTROCHEMICAL DETERMINATION OF RECOMBINASE POLYMERASE AMPLIFICATION PRODUCTS OF DNA." http://eng.biomos.ru/conference/articles.htm 1, no. 19 (2021): 236–38. http://dx.doi.org/10.37747/2312-640x-2021-19-236-238.

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Deoxyuridine triphosphates modified with tyrosine or tryptophan aromatic groups were tested as electroactive ‘labels’ for the electrochemical detection of double-stranded DNA amplicons obtained by recombinase polymerase amplification.

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28

Boyle, David S., Ruth McNerney, Hwee Teng Low, et al. "Rapid Detection of Mycobacterium tuberculosis by Recombinase Polymerase Amplification." PLoS ONE 9, no. 8 (2014): e103091. http://dx.doi.org/10.1371/journal.pone.0103091.

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29

Crannell, Zachary, Alejandro Castellanos-Gonzalez, Gayatri Nair, Rojelio Mejia, A. Clinton White, and Rebecca Richards-Kortum. "Multiplexed Recombinase Polymerase Amplification Assay To Detect Intestinal Protozoa." Analytical Chemistry 88, no. 3 (2016): 1610–16. http://dx.doi.org/10.1021/acs.analchem.5b03267.

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30

Castellanos-Gonzalez, A., A. C. White, P. Melby, and B. Travi. "Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification." Acta Tropica 182 (June 2018): 4–11. http://dx.doi.org/10.1016/j.actatropica.2018.02.002.

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31

Xu, Jialiang, Xiaoxun Wang, Liya Yang, Biao Kan, and Xin Lu. "Rapid detection of mcr-1 by recombinase polymerase amplification." Journal of Medical Microbiology 67, no. 12 (2018): 1682–88. http://dx.doi.org/10.1099/jmm.0.000865.

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32

del Río, Jonathan Sabaté, Ivan Magriñà Lobato, Olena Mayboroda, Ioanis Katakis, and Ciara K. O’Sullivan. "Enhanced solid-phase recombinase polymerase amplification and electrochemical detection." Analytical and Bioanalytical Chemistry 409, no. 12 (2017): 3261–69. http://dx.doi.org/10.1007/s00216-017-0269-y.

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33

Feng, Xinrui, Yan Liu, Yang Zhao, et al. "Recombinase Polymerase Amplification-Based Biosensors for Rapid Zoonoses Screening." International Journal of Nanomedicine Volume 18 (November 2023): 6311–31. http://dx.doi.org/10.2147/ijn.s434197.

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34

Lee, Jeewon, Sunghoon Heo, and Duhee Bang. "Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification." ACS Omega 4, no. 22 (2019): 19953–58. http://dx.doi.org/10.1021/acsomega.9b02886.

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35

Cai, Qiqi, Rui Wang, Zhaohui Qiao, and Wenge Yang. "Single-digit Salmonella detection with the naked eye using bio-barcode immunoassay coupled with recombinase polymerase amplification and a CRISPR-Cas12a system." Analyst 146, no. 17 (2021): 5271–79. http://dx.doi.org/10.1039/d1an00717c.

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An ultrasensitive, rapid, and visual detection platform for Salmonella Typhimurium based on the bio-barcode assay and recombinase polymerase amplification (RPA) coupled with a CRISPR-Cas12a cleavage system is presented.

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36

Rathore, Himankshi, Radhika Biyani, Hirotomo Kato, Yuzuru Takamura, and Manish Biyani. "Palm-size and one-inch gel electrophoretic device for reliable and field-applicable analysis of recombinase polymerase amplification." Analytical Methods 11, no. 39 (2019): 4969–76. http://dx.doi.org/10.1039/c9ay01476d.

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A newly designed handheld one-inch gel electrophoresis-based detection system and recombinase polymerase amplification (RPA) can revolutionize nucleic acid-based molecular diagnostics for people in settings with poor healthcare infrastructure.

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37

Ivanov, A. V., A. V. Zherdev, and B. B. Dzantiev. "ANALYTICAL SYSTEMS BASED ON RECOMBINASE POLYMERASE AMPLIFICATION FOR RAPID DETECTION OF VIRAL, VIROID AND BACTERIAL PLANT PATHOGENS." http://eng.biomos.ru/conference/articles.htm 1, no. 19 (2021): 242–44. http://dx.doi.org/10.37747/2312-640x-2021-19-242-244.

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Test systems have been developed for the detection of phytopathogens, combining recombinase polymerase amplification and membrane test strips. Test systems provide detection of potato virus X, potato spindle tuber viroid, potato blackleg pathogen (Dickeya solani), as well as multi-analysis of three viruses. Amplification is carried out at 37 °C. The analysis time does n ot exceed 30 min.

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38

Lapa, S. A., S. A. Surzhikov, S. A. Blagodatskikh, V. E. Shershov, and A. V. Chudinov. "Recombinase Polymerase Amplification for Rapid Detection of Human Bacterial Pneumonia Pathogens." Молекулярная биология 57, no. 3 (2023): 539–45. http://dx.doi.org/10.31857/s0026898423030072.

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A diagnostic system based on recombinase polymerase amplification (RPA) has been developed to identify six bacterial pathogens of human pneumonia. Species-specific primers have been designed and optimized to conduct a multiplex reaction in one common volume. Labeled primers were used for reliable discrimination of amplification products close in size. Identification of the pathogen was carried out by visual analysis of the electrophoregram. The analytical sensitivity of the developed multiplex RPA was 102‒103 copies of DNA. The specificity of the system was determined by the absence of cross-a

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39

Wu, Shiwen, Wenhan Yu, Xianshu Fu, et al. "Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification." Molecules 29, no. 20 (2024): 4972. http://dx.doi.org/10.3390/molecules29204972.

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Recombinase polymerase amplification (RPA) has emerged as a rapid, efficient, and highly sensitive method for nucleic acid amplification, thus becoming a focal point of research in the field of virus detection. This paper provides an overview of RPA, emphasizing its unique double-stranded DNA synthesis mechanism, rapid amplification efficiency, and capability to operate at room temperature, among other advantages. In addition, strategies and case studies of RPA in combination with other technologies are detailed to explore the advantages and potential of these integrated approaches for virus d

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40

Abd El Wahed, Ahmed, Pranav Patel, Oumar Faye, et al. "Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection." PLOS ONE 10, no. 6 (2015): e0129682. http://dx.doi.org/10.1371/journal.pone.0129682.

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41

Euler, M., Y. Wang, P. Otto, et al. "Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis." Journal of Clinical Microbiology 50, no. 7 (2012): 2234–38. http://dx.doi.org/10.1128/jcm.06504-11.

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42

Martorell, Sara, Sarai Palanca, Ángel Maquieira, and Luis A. Tortajada-Genaro. "Blocked recombinase polymerase amplification for mutation analysis of PIK3CA gene." Analytical Biochemistry 544 (March 2018): 49–56. http://dx.doi.org/10.1016/j.ab.2017.12.013.

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43

Davi, Saskia Dede, Jonas Kissenkötter, Martin Faye, et al. "Recombinase polymerase amplification assay for rapid detection of Monkeypox virus." Diagnostic Microbiology and Infectious Disease 95, no. 1 (2019): 41–45. http://dx.doi.org/10.1016/j.diagmicrobio.2019.03.015.

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44

Martorell, Sara, Luis A. Tortajada-Genaro, and Ángel Maquieira. "Magnetic concentration of allele-specific products from recombinase polymerase amplification." Analytica Chimica Acta 1092 (December 2019): 49–56. http://dx.doi.org/10.1016/j.aca.2019.10.006.

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45

Chi, Yuan-kai, Wei Zhao, Meng-di Ye, Farman Ali, Tao Wang, and Ren-de Qi. "Evaluation of Recombinase Polymerase Amplification Assay for Detecting Meloidogyne javanica." Plant Disease 104, no. 3 (2020): 801–7. http://dx.doi.org/10.1094/pdis-07-19-1473-re.

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Meloidogyne javanica is one of the most widespread and economically important nematodes in many countries, including China. In this study, a recombinase polymerase amplification (RPA) assay was evaluated for the detection of M. javanica based on the sequences of a sequence-characterized amplified regions marker gene segment. The RPA assay specifically detected M. javanica from individual juvenile or adult female, M. javanica-induced galls, and nematodes in the soil samples. The detection limit of M. javanica RPA assay was 1 pg of purified genomic DNA, 0.01 adult female, or 0.1 second-stage juv

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46

Chia, Catherine T., Andrew T. Bender, Lorraine Lillis, et al. "Rapid detection of hepatitis C virus using recombinase polymerase amplification." PLOS ONE 17, no. 10 (2022): e0276582. http://dx.doi.org/10.1371/journal.pone.0276582.

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Over 71 million people are infected with hepatitis C virus (HCV) worldwide, and approximately 400,000 global deaths result from complications of untreated chronic HCV. Pan-genomic direct-acting antivirals (DAAs) have recently become widely available and feature high cure rates in less than 12 weeks of treatment. The rollout of DAAs is reliant on diagnostic tests for HCV RNA to identify eligible patients with viremic HCV infections. Current PCR-based HCV RNA assays are restricted to well-resourced central laboratories, and there remains a prevailing clinical need for expanded access to decentra

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47

Ullah, Hanif, Abdul Qadeer, Muhammad Rashid, Muhammad Imran Rashid, and Guofeng Cheng. "Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development." Parasitology 147, no. 4 (2019): 383–92. http://dx.doi.org/10.1017/s0031182019001665.

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AbstractPathogenic helminth infections are responsible for severe health problems and economic losses worldwide. Timely and accurate diagnosis of helminth infections is critical for adopting suitable strategies for pathogen control. Here, we review recent advances in nucleic acid-based diagnostic methods, including polymerase chain reaction, quantitative qPCR, loop-mediated isothermal amplification and recombinase polymerase amplification, and discuss their advantages and disadvantages for diagnosing helminth infections. In addition, we highlight recent advances in biosensors for the detection

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48

Bondareva, Olga S., Artem A. Baturin, and Anna V. Mironova. "Recombinase polymerase amplification: method’s characteristics and applications in diagnostics of infectious diseases." Journal of microbiology, epidemiology and immunobiology 101, no. 2 (2024): 270–80. http://dx.doi.org/10.36233/0372-9311-470.

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Isothermal amplification techniques have been actively developed in recent years and are gradually introduced into the range of methods for infectious disease diagnostics. One of the fastest isothermal methods is recombinase polymerase amplification (RPA). This review contains information about the principle of RPA, the role of individual reaction components and primer design considerations. It provides information on characteristics of various methods of RPA results detection, effects of inhibitors, temperature and agitation on the efficiency of reaction. Approaches to quantitative and multip

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49

Frimpong, Michael, Hubert Senanu Ahor, Samuel Asamoah Sakyi, Bernadette Agbavor, Emmanuel Akowuah, and Richard Odame Phillips. "Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients." Diagnostics 9, no. 4 (2019): 204. http://dx.doi.org/10.3390/diagnostics9040204.

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Isothermal amplification techniques such as recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) for diagnosing Buruli ulcer, a necrotic skin disease caused by Mycobacterium ulcerans, have renewed hope for the molecular diagnosis of clinically suspected Buruli ulcer cases in endemic districts. If these techniques are applied at district-level hospitals or clinics, they will help facilitate early case detection with prompt treatment, thereby reducing disability and associated costs of disease management. The accuracy as well as the application of these mo

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Wu, Xinyan, Shuting Chen, Zixin Zhang, et al. "Development of Recombinase Polymerase Amplification Combined with Lateral Flow Strips for Rapid Detection of Cowpea Mild Mottle Virus." Plant Pathology Journal 39, no. 5 (2023): 486–93. http://dx.doi.org/10.5423/ppj.oa.02.2023.0033.

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Abstract:

Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks. With the advancement in isothermal recombinase polymerase amplification and lateral flow strips technologies, more rapid and sensitive methods have become available for detecting this pathogen. In this study, we have developed a reverse transcription recombinase polymerase amplification combined with lateral flow strips (RT-RPA-LFS) method for the detection of CPMMV, specifically targeting t

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