Journal articles on the topic 'DRY BIOLOGICAL FERMENT'

The present paper features processes of serum biotransformation. The serum was obtained from triticale extract and pea flour after protein concentrates of increased biological value had been extracted. The research objective was to obtain microbial and vegetable feed concentrates by using a composition of Saccharomyces cerevisiae121 yeast and the yeast-like fungus Geotrichumcandidum 977. The mass fraction of protein in the two-component composites was 75–80% of the dry matter. The score of the first and the second limiting amino acids (lysine and threonine) equaled 103–113%, and that of the third acid (sulfur- containing) was 71–72%. The chemical composition of the composites corresponded to the ‘Concentrates’ group; the values of their functional and technological properties were typical of concentrates from other types of grain crops. The study revealed some cultures that are able to actively develop in serum, which is a secondary product of processing the extract after protein isolation. A symbiotic ferment was prepared from the fungus Geotrichumcandidum 977 and the yeast Saccharomyces cerevisiae 121, which ensures the growth of biomass in a carbohydrate- and nitrogen-containing medium. Proteins were isolated under the action of amylase, glucoamylase, cellulose, and xylanase. The amount of high-molecular compounds (dextrins) and trioses (raffinose) released from the interaction with protein and non-starch polysaccharides decreased 2–4 times in the solution. The amount of glucose, disaccharides, xylose, and galactose increased 2–10 times, compared with the original extracts. The serum remaining after the removal of the main mass of the protein was enriched with low molecular weight mono- and oligosaccharides, which positively affected the growth of microorganisms. The mass fraction of proteins in the microbial-vegetable composite obtained from the extract with the triticale proteins and pea flour ratio of 1:5 was 15% higher than at the ratio of 1:3. Microbial and vegetable concentrates with a mass fraction of protein of 55.8–75.1% of dry matter can be used in fodder production as a protein-carbohydrate additive. Protein composites made of protein triticale and peas with a complementary amino acid composition can improve the biological value and performance of food products.

The objective of this study was to evaluate the effects of bacterial and chemical additives on the number of lactic acid bacteria (LAB) and on fermentation indicators in whole maize silage at 1, 3, 5, 10, and 90 days of fermentation. Maize forage was harvested at approximately 34% dry matter (DM) and treated with (1) no additive (control; C); (2) bacterial inoculant (2 g/t of forage; B) containing the homofermentative LAB Lactobacillus plantarum, Lactobacillus paracasei, and Pediococcus pentosaceus (1.5 × 10¹¹ cfu/g of inoculant); and (3) chemical additive (4 l/t of forage; CH) containing formic acid, propionic acid, ammonium formate, and benzoic acid. Both treatments decreased pH of silage at day 1 of ensiling (P < 0.05), and the lowest value of 4.34 was observed in the CH-treated silage. All silages were well fermented and had pH < 4.0 by day 10 of fermentation. The concentration of lactic acid and the lactic acid : acetic acid ratio increased over time in all treatment groups, and the highest values were 87.5 and 3.62 g/kg of DM, respectively, observed for group B at day 90 (P < 0.05). The concentrations of water-soluble carbohydrates were higher (P < 0.05) for CH compared to C and B at days 3, 5, 10, and 90 of fermentation. The CH silage had fewer LAB (P < 0.05) than did either C or B silages regardless of the days of fermentation. Both additives used in the present study improved fermentation dynamics of the whole crop maize silage.

Anaerobic fermentation is a process in which almost any organic mass may be transformed into an energetically rich biogas and a fermentation residue. Only strictly anaerobic microorganisms enter into the process; thus the process may take place only in a hermetically sealed environment. With regard to the world wide situation, where the increase in the proportion of energy from sustainable sources is in demand, anaerobic fermentation offers the possibility of transforming farm waste, farm products and municipality waste of biological character into electricity. This electricity may subsequently become an interesting source of income. The system may be proposed to agricultural companies as well as to municipality corporations. The process of fermentation may be carried out as dry fermentation or as liquid fermentation. Dry fermentation, working with materials where the percentage of dry matter exceeds 15 %, is the topic of this paper. This method has been frequently discussed as a method of processing organic material without waste water and thus the volume of material as well as the size of the biogas plant considerably decreases. To enable progress in the process, it is necessary to use a biologically active liquid solution containing the essential micro-organisms, often termed “percolate”. To activate a fresh substrate, fermented material adulterant containing cultivated microorganisms from previous processes is used; the ratio in which it is used is approximately one third to one fifth. “Percolate strategy” is another phrase used for sustaining the anaerobic fermentation; material is sprinkled on the percolate in the precisely defined cycles. In addition, the biologically active liquid solution contains organic substances washed out from the fermented material. With regard to its amount, this paper has become an impulse for the research in the amount of biogas which may be subsequently produced from the percolate in the so-called biofilm reactor. An external reactor with a cultivated bacterial biofilm on an immovable carrier with the percolate flowing through it has been constructed in laboratory conditions for this purpose. The choice of suitable percolate strategy (this means the frequency of sprinkling) and the amount of percolate directly influences the process of anaerobic fermentation.

The influence of the ratio of fermented rice straw, rice bran and cassava solid waste upon the In vitro digestibility and rumen fermentation productABSTRACT. Chemical and physical treatments have been attempted to improve the utilization of agro industrial wastes, but the result is not efficient and caused pollution. Besides, biological treatments using the microbes have been used to improve the nutritive value and utilization of agro industrial wastes. The current experiment was conducted to find out the optimal ratio of fermented rice straw, fermented rice bran and fermented tapioca waste. There were five kinds of ratio of fermented rice straw, fermented rice bran and fermented tapioca waste, namely: K1 (70% fermented rice straw + 15% fermented rice bran + fermented tapioca waste), K2 (60% fermented rice straw + 20% fermented rice bran + 20% fermented tapioca waste), K3 (50% fermented rice straw + 25% fermented rice bran + 25% fermented tapioca waste), K4 (40% fermented rice straw + 30% fermented rice bran + 30% fermented tapioca waste) and K5 (30% fermented rice straw + 35% fermented rice bran + 35% fermented tapioca waste) An in vitro technique, using completely Randomize Block Design was applied and each treatment was repeated four times. Variables measured were Dry matter and Organic matter digestibility and rumen fermentation products (volatile fatty acid and N-NH3 concentration). The dry matter digestibility of K1, K2, K3, k4 and K5 was 29.39, 31.27, 32.33, 33.71 and 34.82%, respectively. The organic matter digestibility of K1, K2, K3, K4 and 5 was 30.82, 31.27, 32.73, 34.94, and 34.92, respectively. Volatile fatty acid concentrations of K1, K2, K3, K4 and K5 were 95.19, 91.77, 87.21, 104.31, 106.59 mM/l, respectively. N-NH3 concentrations of K1, K2, K3, K4 and K5 were 0.97, 0.93, 0.93, 1.00, 1.04 mM/l, respectively. Significant difference (P0.01) was only found in dry matter digestibility among treatments, while others variables were not significantly different among treatments. It was indicated that the optimal ratio was K4.

Changes in amino acid profile of alfalfa silage preserved with chemical or biological additives were studied in fresh and wilted silage. The chemical additive was formic acid and the biological additive consisted of Lactobacillus rhamnosus, L. plantarum, L. brevis, L. buchneri and Pediococcus pentosaceus. Second cut alfalfa (Medicago sativa L.) was harvested at the bloom stage, ensiled in mini silos (15 dm3) and fermented at 20–23 °C for 12 weeks. The dry matter of the fresh silage was 228 g . kg−1 and 281.6 g . kg−1 for the wilted before ensiling. The amino acid content was estimated by using an automatic amino acid analyzer AAA (INGOS Prague). The results of the experiments indicated that amino acid breakdown was inhibited by increased dry matter and the use of chemical and biological additive. Additionally, the content of amino acids was found to change in relation to the degree of wilting and formic acid treatment yielded the lowest amino acid breakdown. The amino acid breakdown was also reduced by biological preservative especially in the silage with a higher level of dry matter content.

Twelve wether sheep were used in a 2 x 2 factorial experiment in which voluntary intake, diet digestibility, and eating and ruminating behaviour were compared when sheep were offered lucerne (Medicago sativa) as either wilted silage or hay. Chemical characteristics suggested that the lucerne silage was well fermented, and chemical compositions of the silage and hay were similar. Voluntary intakes were not significantly (P<0.05) different and were 1201 and 1220 g/day for silage and hay, respectively. Digestibilities of dry matter and organic matter were similar for both feeds, as were chewing behaviour and rumen physical function. Apparent crude protein digestibility was significantly (P<0.05) higher for silage than for hay (73.8 v. 70.3%). It was concluded that well-preserved lucerne silage has at least as high a feeding value as well-made lucerne hay.

Objective: The present study evaluated the subchronic toxicity of Lactobacillus mediated fermented Phyllanthus emblica fruit juice (FPJ) using a rat as a model system.Methods: FPJ was prepared, and estimated the changes in pH by pH meter, and microbial load by a plating method. Rats were fed with different dose of FPJ for 60 days. The changes in the body mass were noted. The blood and organs of the experimental rats were collected, after 60 days of intervention. Then, they were analyzed for the selected hematological and biochemical parameters by following standard hospital protocols.Results: The pH of FPJ after 30 days of fermentation was 3.16. FPJ was rich in probiotic Lactobacillus spp. (7.23 Log CFU per mL) without contamination. The supplementation of FPJ was not significantly affected the body weight of the experimental animals, except the female rats in posteffective dose (PED) group showed significant changes (20.83±8.49 g) compared to control (40±17.22 g). The internal organs of the rat were not affected by the FPJ supplementation. The changes observed in blood urea nitrogen, creatinine, cholesterol, triglyceride, aspartate aminotransferase; alanine aminotransferase, and alkaline phosphatase level of experimental rats, both male and female, were not significantly differed from the respective controls. The average of lymphocytes level was significantly increased in continuous dosing group of males and females. Interestingly, the increase in red blood cell and hemoglobin (HGB) were statistically significant for ED group and PED in both sexes, except for females with no effect on HGB content.Conclusion: The prepared FPJ was enriched with probiotic Lactobacillus spp. The supplementation of FPJ (up to 9 mL/kg/day) for 60 days was not significantly influenced the body weight, internal organs, biochemical and hematological parameters of experimental rats (both male and female). The results revealed that FHJ is suitable for the human consumption.

The integrated use of aspen biomass can ensure the effective development of forestry and timber processing industries, reduce the share of waste. From the bark of aspen we obtained aqueous and water-alcohol extracts containing a complex of extractive substances possessing biological activity and representing practically all classes of organic compounds found in plants (vitamins, enzymes, proteins, fats, essential oils, etc.). As a raw material, a crushed aspen bark with a moisture content of 5% and an equivalent particle diameter of 2 mm was used. Extractants – water distilled and water-alcoholic solution 1:1. Extraction conditions: hydromodule– 10 g/g, temperature – 40°C, ultrasound frequency – 44 kHz. The effect of water-alcohol extracts on the fermentation of sucrose by beer yeast S-33 was studied. The expediency of using water-alcohol extract of aspen cortex at a dosage of 1% of the nutrient medium is shown. At the same time ferment activity of yeast increases, sugar consumption decreases by the side processes of fermentation, the economic coefficient showing the yield of ethanol from a unit of substrate is increased by 5,5% in comparison with the control. The kinetic characteristics of the fermentation process are calculated. The maximum specific growth rates of yeast and sugar consumption are observed when using water-alcohol extract of bark of aspen at a dosage of 1% and are 0,0052 h-1 and 0,049 g/(g-h), respectively. On the basis of the conducted studies, it can be concluded that the water-alcohol extract of the aspen bark should be used at a dosage of 1% of the nutrient medium. At the same time ferment activity of yeast increases, sugar consumption decreases by the side processes of fermentation, the economic coefficient showing the yield of ethanol from a unit of substrate is increased by 5,5% in comparison with the control. Industrial use of this extract in the production of beer will shorten the duration of the main fermentation for 1 day, increase the capacity of the fermentation unit and reduce the specific rates of energy consumption.

<span style="font-family: Times New Roman; font-size: small;"> </span><p>Functional food or medicinal food is any healthy food claimed to have a health-promoting or disease-preventing property beyond the basic function of supplying nutrients. Three nutritional preparations including wheat powder salt solution (WPSS), milk-wheat solution (MWS), fermented milk (FM) and fermented soymilk (FSM) were evaluated for their anti-diarrheal activity by oral administration in model of Castor oil induced diarrhea in rats. Oral rehydration solution (ORS) was used as positive control. The fermented products were prepared using a mixture of <em>Lactobacillus acidophilus</em> ATCC 4356: and <em>Bifidobacterium bifidum</em> ATCC 700541 (1:1 v/v) to obtain a final level of 10^7-8 CFU/ml after incubation at 37°C. Beside the gain body weight (BW), certain biochemical parameters such as total protein, albumin, globulin, urea, creatinine, alanine amino-transferase (ALT), aspartate amino-transferase (AST), sodium, potassium, magnesium, iron and phosphorus were determined.</p> <p>According to follow the diarrheal symptoms including stool frequency, stool characteristics and BW, rats administrated with FSM were recovered from diarrhea (on the 3^rd day) faster than other groups followed by those subjected with FM and CY. The ORS-positive control group rats were recovered on the 6^th day, while diarrheal symptoms still appeared on the negative control rats (subjected with basal diet only; without ORS) with 16% death rate. Minerals, especially sodium, potassium, magnesium and phosphorus, were the most significant biochemical parameters for following recovery from diarrhea. The normal levels of these minerals were recovered in the blood serum at the end of experiment in rats administrated with the fermented products (FSM, FM and CY). Some renal functional parameters were suggested to follow diarrhea, but all studied liver functional parameters were not significantly recommended.</p><span style="font-family: Times New Roman; font-size: small;"> </span><span style="font-family: Times New Roman; font-size: small;"> </span>

In this work, the influence or probiotic strainL. casei01 (Sacco, Italy) on biogenic amines concentrations during fermentation, ripening and storage of fermented sausages “Herkules” was studied. Two amounts of probiotic culture were added into sausages, 0,25 % and 0,40 %, respectively. Negative controls without probiotic were also made. Dry sausages were taken from two different producers (A, B). Determination of biogenic amines tyramine, histamine, putrescine and cadaverine concentration was used using HPLC method with UV detection. The content of biogenic amines was monitored during fermentation (0–28 days) and storage (28–49) of fermented sausages.In both producers, when 0,25 % of probiotic strains was added, the numbers ofL. caseireached 104CFU/g during the fermentation (0–28) and they remained relatively constant during the sto­ra­ge (28–49). When 0,40 % of probioticL. casei01 was added, the amount ofL. caseicells were around 106 CFU/g during the whole fermentation and storage period.A positive influence on the reduction of biogenic amines was observed in both producers. In negative controls without probiotic, the higher concentration of tyramine, histamine, putrescine and ca­da­ve­ri­ne was detected in comparison with probiotic sausages.Differences between dry sausages with 0,25 % and 0,40 % probioticL. casei01 were also determined. In producer A, the concentration of putrescine and cadaverine in 0,40 % probiotic sausages were statistically lower in comparison with probiotic sausages where 0,25 %L. caseiwas applied. In producer B, the concentration of all monitored biogenic amines were statistically lower in 0,40 % probiotic sausages when compared with 0,25 % probiotic sausages.

Mixed cultures of anaerobic micro-organisms were derived from feral goat rumen fluid (FGRF) using a laboratory fermentor to selectively culture microbes actively degrading mulga, and were evaluated as rumen inocula in digestion and liveweight studies with mulga-fed sheep. When placed in the rumen of sheep, FGRF enhances mulga digestion; however, limited supplies of feral goats, the labour involved in locating and mustering goats, and likely variations in the microbial composition of FGRF between animals and localities make the production of an in vitro cultured inoculum a desirable alternative to enable widespread adoption. The cultured inoculum significantly (P < 0·05) improved nitrogen digestion and retention in mulga-fed sheep by 16 and 76%, respectively. Inocula consisting of simplified mixtures of bacteria isolated from sheep, feral goats, and native marsupials did not affect mulga digestion. In the first of 2 liveweight studies, sheep inoculated with the fermentor inoculum lost significantly less weight than uninoculated sheep for the first 57 days (0·3 v. 4·6 kg); however, after 83 days the difference in the rate of liveweight loss between the fermentor inoculum group and the uninoculated sheep was not significant (53 v. 95 g/day). In the second study, liveweight loss was not significantly reduced by the fermentor inoculum. An inoculum based on FGRF, and produced in vitro using a fermentor, is potentially valuable to grazing enterprises reliant on mulga-fed sheep. However, problems in generating a consistent inoculum need to be addressed before such an inoculum can be generally considered.

Rice bran is agriculture waste that easy to find. Means to increase biological value of rice bran can do with decrease of highly crude fiber. Treatment that to do with fermentation use rumen fluid from cows. Rumen fluid potential is easy to find in slaughterhouse, this rumen fluid much to never utilization. This purpose of the research for evaluation of rice bran nutrition value that fermentating used rumen fluid, with different levels and long time incubations for get lower crude fiber and higher crude protein content. Methods of this research used Laboratory experiments with Factorial-Random Complete Design 4x4 and 6 repetitions. First factor is rumen fluid levels (0, 30, 40, 50 %/w) and second factor is long incubations (0, 24, 48, 72 hours). This result of ANOVA analysis to show that increment rumen fluid levels and long incubations on rice bran fermentating process can decrease Dry Matter, Organic Matter, Crude Fiber, NDF, ADF and NDR content; as well as increase Crude Protein content but not increase Crude Fat content. The best interaction between rumen fluid levels and long time incubations is 30 %/w with 72 hours long time incubations, with 10.28±0.1 % of Crude Protein, 6.46±0.07 of Crude Fat and 22.31±0.4 of Crude Fiber content.

PHA (polyhydroxyalkanoate) production was attempted with SBRs from food waste. Seed microbes were collected from a sewage treatment plant with a biological nutrient removal process, and acclimated with synthetic substrate prior to the application of the fermented food waste. Laboratory SBRs were used to produce PHA with limited oxygen and nutrients. The maximum content of 51% PHA was obtained with an anaerobic/aerobic cycle with P limitation, and the yield was estimated to be about 0.05 gPHAproduced/gCODapplied or 25 kg PHA/dry ton of food waste, assuming more than 40% of the PHA contents were recoverable. PHB/PHA ratios were 0.74 to 0.77 due to the higher acetate concentrations. Economical analysis seemed to suggest the PHA produced from the food waste could be an alternative material to produce the biodegradable plastic to be used for the collection bags for solid waste.

Abstract Anti-nutritional factors (ANF) and antigenic proteins present in soybean meal (SBM) limit its application in piglet diets. Therefore, protein sources of high biological value are used as an alternative to replace SBM, which became the pre-initial feed the most expensive diet in pork production. The main problems caused by the presence of ANF are related to poor digestion, absorption and nutrient utilization resulting in increased diarrhea, intestinal inflammation, and consequent lower growth performance. ANF in soybean might be inactivated through heat treatment but also, it may reduce the availability of some amino acids when overheated. The process of fermentation may enhance the nutritional value of soybean by increasing the bioavailability of nutrients and reducing ANF. The objective of this study was to evaluate the nutrients digestibility of fermented soybeans for nursery piglets. Twenty pigs (barrows), initial weight of 11 kg, were housed in metabolic cages and had their feces and urine collected for analysis. Piglets were assigned in ten replicates and two experimental diets, basal diet and fermented soybean diet (80% of the basal diet and 20% of the fermented soybean). The total amount fed was determined based on metabolic weight parameters. Ferric oxide was used as a fecal marker. The coefficient of digestibility was used to estimate the nutritional value trough analyzing dry matter digestibility (DMD), crude protein digestibility (CPD), calcium digestibility (CD), phosphorus digestibility (PD), digestible energy (DE) and, metabolizable energy (ME). The digestibility coefficients were 91.02% for DMD; 93.74% for CPD; 76.10% for CD; 54.62% for PD; and 86.73% for DE. ME was determined in 3589 kcal kg-1. Crude protein was determined in 59.64%. The nutritional profile of the fermented soybean shows a great potential to be used in initial diets of piglets due to their high level of nutrients digestibility.

The eight-week feeding trial was conducted to evaluate the nutrient digestibility and growth performance of broiler chickens fed raw or processed Senna obtusifolia seed meal (SOSM). Six experimental diets were compounded to contain 0% SOSM and 20% each of the raw, boiled, soaked, sprouted and fermented SOSM respectively. Two hundred and sixteen (216) broiler chicks were randomly allotted to six (6) dietary treatments in a randomised complete block design with three (3) replicates containing 12 chicks each. Data were collected on nutrient digestibility, feed intake, weight gain, feed conversion ratio and mortality. The result of productive performance indicated that feed intake, weight gain and feed conversion ratio were significantly (P<0.05) depressed in broiler chickens fed raw, soaked and sprouted Senna obtusifolia seed meal. However, broiler chickens fed fermented SOSM showed better (P<0.05) productive performance that was close to those fed the positive control diet (0% SOSM). The mortality rate did not reveal any particular trend. However, the low mortality rate recorded in the different treatments indicated broiler chicken can tolerate up to 20% of either raw or processed SOSM. Broiler chickens fed raw SOSM recorded lower values for dry matter (63.06%), crude protein (52.46%), ether extract (53.88%), crude fibre (30.72%) and nitrogen-free extract (46.11%) digestibility than those fed the other processed SOSM. The nutrient digestibility of broiler chickens fed fermented SOSM was significantly (P<0.05) better followed by those fed boiled Senna obtusifolia seed meal. For instance, broiler chickens fed fermented Senna obtusifolia seed meal recorded the highest crude protein and ether extract of 69.91 and 70.03% compared to the other processing methods. It can be concluded that 20% fermented SOSM can be incorporated in the diets of broiler chickens with acceptable depreciation in biological performance. Slightly lower levels of boiled SOSM can be included in the diet of broiler chickens but the levels should be ascertained in further studies.

Despite the known beneficial impacts of estrogen used as hormone replacement therapy to ameliorate signs of skin aging in postmenopausal women, its compliance rates are low. A significant amount of estrogen may be absorbed into the blood circulation and can lead to systemic actions. Soy isoflavone exhibits biological activities similar to synthetic estrogen because it is a heterocyclic phenolic compound. The disadvantage of most topical ingredients based on isoflavone is that they contain biologically inactive glycoside forms, which must be converted to a readily absorbed aglycone for the topical application. The purposes of this study were to develop niosomes-loaded Aspergillus oryzae-fermented soybean extract (FSE) to enhance skin absorption with proven systemic side effect compared to estrogen application. Skin hydration and viscoelasticity of 75 days post-ovariectomized (OVX) Wistar rats following 84-day topical treatment with various tested gel formulations containing fermented soybean extract (FSE) were evaluated. The tested formulations were gel + FSE nanoniosomes, gel + FSE microniosomes, gel + FSE (200 µg FSE/9 cm2/rat), gel + blank nanoniosomes (a negative control), and gel + 17β-estradiol (E2) nanoniosomes (a positive control, 20 µg E2/9 cm2/rat). Changes in vaginal cornifications and weights of uteri, livers, and kidneys in the OVX rats and signs of primary skin irritation in the rabbits were evaluated for their toxicities. Results showed that FSE-loaded nanoniosomes improved the skin hydration and viscoelasticity better than gel + FSE microniosomes and gel + FSE, respectively, but lower than those of gel + E2 nanoniosomes (p < 0.05). Unlike all gel + E2 nanoniosomes, the FSE formulations showed no changes in vaginal cells and weights of uteri, livers, and kidneys and no signs of skin irritation. In conclusion, The FSE niosome-based gels should be promising candidates for delivering phytoestrogens against signs of skin aging with no systemic toxicities.

ABSTRACT The proper design of DNA microarray experiments requires knowledge of biological and technical variation of the studied biological model. For the filamentous fungus Aspergillus niger, a fast, quantitative real-time PCR (qPCR)-based hierarchical experimental design was used to determine this variation. Analysis of variance components determined the contribution of each processing step to total variation: 68% is due to differences in day-to-day handling and processing, while the fermentor vessel, cDNA synthesis, and qPCR measurement each contributed equally to the remainder of variation. The global transcriptional response to d-xylose was analyzed using Affymetrix microarrays. Twenty-four statistically differentially expressed genes were identified. These encode enzymes required to degrade and metabolize d-xylose-containing polysaccharides, as well as complementary enzymes required to metabolize complex polymers likely present in the vicinity of d-xylose-containing substrates. These results confirm previous findings that the d-xylose signal is interpreted by the fungus as the availability of a multitude of complex polysaccharides. Measurement of a limited number of transcripts in a defined experimental setup followed by analysis of variance components is a fast and reliable method to determine biological and technical variation present in qPCR and microarray studies. This approach provides important parameters for the experimental design of batch-grown filamentous cultures and facilitates the evaluation and interpretation of microarray data.

The biological route to produce xylitol from Oil Palm Empty Fruit Bunches (EFBs) comprises of EFBs pretreatment, enzymatic hydrolysis, fermentation, and downstream separation of the produced xylitol. Due to the specificity in the hemicellulose composition of EFBs, a xylanase enzyme that has a high affinity to EFBs is required to hydrolyze the EFBs into xylose. In this research, the influences of aeration, humidity, and mixing in xylanase production were mapped. The xylanase production was performed by Aspergillus fumigatus ITBCCL170 in a solid-state fermentation using a tray fermenter with EFBs as the substrate. The optimal configuration was further scaled up into xylanase production using 1000 g of EFBs as the substrate. The results showed that the highest enzyme activity was 236.3 U/g EFB, obtained from the use of humid air airflow of 0.1 LPM, and mixing was performed once a day. The scaling up resulted in a lower xylanase activity and call for a better design of the fermenter.Keywords: aeration, humidity, mixing, OPEFBs, tray fermenter, xylanase, xylitol

Pasture was ensiled with increasing amounts of whole barley in stacks [0, 75 (SLB), 150 (SMB) kg/t fresh pasture] or in 200-L drum silos 10, 75 (DLB), 150 (DMB), 225 (DHB) kg/t fresh pasture]. All silages were well fermented with low pH values and low ammonia concentrations. The addition of barley significantly (P<0.05) increased the dry matter content of the resultant stack silages by 29% (SLB) and 54% (SMB) and significantly (P<0.05) increased residual water-soluble carbohydrate levels in the SMB silage compared with untreated stack silage. Incorporation of barley with pasture in 200-L silos significantly (P<0.001) increased the dry matter of the resultant silages and significantly (P<0.01) reduced effluent production by 55, 93, and 100% for DLB, DMB, and DHB compared with the untreated silo silage. Dry matter, nitrogen, and lactic acid concentrations in effluent did not differ significantly, although total losses of these components were significantly (P<0.01) higher in the untreated silage.| The stack silages were fed to beef steers (293 kg) and compared with silage diets containing equivalent levels of rolled barley mixed with untreated silage at feeding. Irrespective of method of feeding barley, animal performance improved compared with feeding a silage-only diet. At equivalent levels of barley inclusion, liveweight gains were higher and feed conversion ratios lower with the rolled barley diets. The results indicate that the addition of barley to low dry matter pasture during ensiling can reduce effluent production and the loss of soluble nutrients; however, it is likely that the grain will need to be processed to maximise subsequent animal production.

Abstract Feed is an element that really support the activities of aquaculture, there for the feed that is available must be adequate and meet the needs of the fish. Efforts to reduce feed costs, some farmers use alternative feed ingredients as a substitute for feed ingredients. Peanut leaves in the form of flour can be used as fish feed in pellet form. Peanut is potential biological resources to be used as feed to the waste produce sufficient forage nutritional value. Peanut leaves nutrition consist of, the dry matter 96,2754%, 29,7397% crude fiber, 17,2475% crude protein (Unit Inspection Services Consulting and Training of Faculty of Veterinary Medical Laboratory Airlangga University, 2014).This research been used Completely Randomized Design (CRD) method with five treatments and four replications. The treatment used were control (P0), the provision of Enterobacter cloacae WPL 111 5% (P1), 10% (P2), 15% (P3) and 20% (P4) with each repeated four times. Parameters observed were content of crude protein and crude fiber after fermentation for seven days in anaerob facultative. Data about the contain of crude protein and crude fiber obtained from this studied were analyzed with analysis of variants to determine the effect of treatment. Different between treatments were tested with Duncan;s multiple range test (DMRT). These results indicate that administration Enterobacter cloacae WPL 111 10% gave significant effect of the increase in crude protein content in the fermented leaves peanut and administration Enterobacter cloacae WPL 111 5% gave significant effect of the decrease in crude fiber content in the fermented leaves peanut. The suggested dosage to reduce the content of crude fiber and increase the crude protein content in the fermented leaves peanut is 10%.

The effectiveness of a powdery preparation of a Streptomyces sp. isolate as a seed dressing agent against seed-borne Alternaria brassicicola on different Brassica species was investigated in the study. The preparation was made by freeze-drying and milling the biomass produced in a fermentor into a form suitable for use as a dusting agent. Seed dressing was 80—90 % successful in controlling damping-off from seeds artificially infected with A. brassicicola. The effectiveness of dressing remained unchanged on seeds stored under dry conditions for 5—6 weeks, but subsequently decreased slowly and was ca. 50 % six months after dusting. Streptomyces dressing controlled, in a manner comparable to chemical dressing with thiram, damping-off caused by Alternaria fungi on seedlings which were grown from commercial seed lots of different origin. The results of biological control of damping-off did not vary in the peat lots of different origin whose natural disease suppressivity varied considerably. The control result was the same or better than chemical dressing with thiram. The acidity of the substrate (pH 4.8—8.6) had no effect on the effectiveness of biological control. The results obtained against Alternaria damping-off were the same in other substrates clay, fine sand and mull as in peat.

Abstract Objectives Previous reports indicate that intake of some soy products may be associated with improvements in blood lipids but few studies examined fermented soy foods. Q CAN PLUS® is a functional food product derived from fermented soybeans. To evaluate the effects of fermented soy on cardiovascular risk factors, we implemented a clinical trial to compare the effects of the fermented soy powder (Q CAN PLUS®) against a placebo (sprouted brown rice powder) in individuals at risk for cardiovascular diseases. Methods Twenty-seven adults at risk for cardiovascular diseases (29–75 years old; 78% female; mean BMI [32.3 kg/m2 ± 7.3]) were randomized to consume either 2 pouches (12–15 g powder/pouch) per day of fermented soy powder (Q CAN PLUS®), or a sprouted brown rice powder for 12 weeks each, in a crossover design trial. The intervention phases were separated by a two week wash out period. Measurements and biospecimens were taken at baseline and at weeks 11 and 12 of each phase. Assessment included blood lipids, blood pressure, blood glucose, blood insulin, and anthropometric measures. For each of the outcomes, a mixed model was fitted to compare changes from pre- to post-treatment and between treatments. The models included treatment, time, interaction between treatment and time, phase, visit within phase and enrollment period as fixed effects terms and subjects as random effects terms. Results Between treatment results from the mixed model analysis show that fermented soy powder intake significantly reduced serum cholesterol (mean change −8.95 mg/dL; P = 0.0024), LDL (mean change −6.96 mg/dL; P = 0.0317), and HDL (−1.29 mg/dL; P = 0.0036) compared with the sprouted brown rice powder. Within treatment results indicated that fermented soy powder significantly reduced total cholesterol (P = 0.0073) and LDL (P = 0.0132), while reductions in HDL were not statistically significant. Within treatment results were not statistically different for the placebo phase except for an increase in HDL (mean change 3.61; P = 0.0026). Conclusions These data indicate that regular intake of fermented soy powder results in a reduction in serum total and LDL cholesterol which may have beneficial implications for the reduction of cardiovascular risk factors in adults. Funding Sources BESO Biological Research Inc. Diamond Bar, CA, USA.

The need to include goat milk and products based on it in the diet of children of all age groups is justified by the high nutritional and biological value, low allergenicity and nutritional value. The aim of the study was to evaluate the clinical efficacy fermented milk products based on goat’s milk on children’s health. A study of the effect of fermented milk products based on goat’s milk on the skin condition, fecal microbiology, blood biochemical parameters, the activity of transaminases ALT, AST, total bilirubin, urea, total protein, total iron-binding capacity of blood serum, the level of albumin, glucose, indicators of the cellular and humoral links immunity, as well as the level of immunoglobulin E with a two-month consumption of products by 30 children of the specialized orphanage in Nur-Sultan, aged 8 months to 4 years. The analysis of the obtained results indicates that after 60-day intake of fermented milk products by children of a specialized orphanage in NurSultan, there was a positive trend in changes in the skin condition, functional disorders of the digestive system were leveled, and dysbiotic disorders of the intestine decreased. On the part of blood biochemical parameters, a positive dynamic was also noted in the change in the level of hemoglobin, erythrocytes, hematocrit, levels of protein, calcium and iron, as well as hepatic transaminases. The level of immunoglobulin E in the blood decreased, indicating a decrease in the allergic load on the body, the indicators of the cellular link of immunity normalized.

A two-stage anaerobic-aerobic sequencing batch reactor (SBR) system (PAF-SBR) was developed to enhance biological phosphorus removal in the sequencing batch reactors. The system performance was evaluated against a conventional SBR system in parallel lab-scale reactors at room temperature, using the degritted raw wastewater as the feed. The SRT for the anaerobic SBR which is named PAF (Primary Acid Fermenter) was 12 days, and for both the BNR reactors was 10 days. All reactors were run at 3 cycles per day. A significant difference (P=0.01) was observed between the performances of the two systems. The Ortho-P concentration in the effluent from the PAF-SBR was mostly below 0.5 mg/L while in the conventional SBR was generally above 1.5 mg/L. Lack of availability of carbon (mean VFA/PSol.=1.1) and long anoxic/anaerobic period were the major causes of inefficient removal of phosphorus in the conventional SBR system. The use of anaerobic stage however increased the mean VFA/PSol. to 11.3 which enhanced Bio-P removal in the PAF-SBR system. Prefermentation also improved the sludge consistency and settleability in the following SBR unit. The results indicated that by using the perfermentation step, the anoxic/anaerobic period in the BNR-SBR could be controlled and reduced to less than 50 minutes, which would reduce the total cycle time from 8 hr to 6 hr.

Nutrients removal efficiencies highly depend on the presence of biodegradable organic carbon in the biological nutrient removal (BNR) process but most domestic wastewater in Korea has shown a low C/N ratio and has a small amount of biodegradable COD (chemical oxygen demand). On the other hand, about 11,577 tons of food waste that contains a lot of organic material has been produced in Korea per day. The feasibility and applicability of anaerobically fermented leachate of food waste (AFLFW) as an external carbon source was examined in the laboratory-scale BNR process at different operation conditions with synthetic wastewater and domestic sewage. As the addition of AFLFW increased, the average removal efficiencies of SCOD, T-N, T-P changed from 96%, 60%, and 2% to 90%, 77%, and 67%, respectively. From anoxic nitrate utilization tests, it was observed that once the readily biodegradable COD (especially VFAs) was depleted, the denitrification rate reduced from 8.2 mg NO3-N/g VSS/hr to 0.7 mg NO3-N/g VSS/hr. From the molecular size distribution test, it was concluded that about 60% of soluble COD in effluent, which was considered to originate from AFLFW, had a large molecular size (&gt; 30kDa) that was not used by microorganisms.

This paper deals with the optimization of the anaerobic fermentation process of maize silage using the non-liquid technology. The aim of the paper is to increase the knowledge necessary for more efficient running of biogas stations using batch technology of non- liquid fermentation. The preferable ratio of initial materials, fresh maize silage and maize silage after partial fermentation, was verified within this experiment. Dynamics of the process, especially the quantity and quality of produced biogas has been monitored during the experiment. All the experiments have been done in the batch-system fermentors of volume of 0.48 m3 equipped with an independent and indirect circuit of water heating, the heat has been transferred to the material throught the steel wall of the fermentor. The material has been tested in the mesophilic conditions with the temperature of the 38 °C. The retention time of material in fermentors has been 27 days. The results of our experiments reveal the fact that the maize silage of lower mix ratio produced about 22.8 % more of biogas counted to one kilogram of dry matter than the maize silage of higher mix ratio.

Conventional pump-and-treat strategies for dealing with groundwater contamination are both energy- and time-consuming. Potential passive biological techniques are of interest to remedy the massive volume of total petroleum hydrocarbon (TPH)-contaminated groundwater worldwide. In this study, novel biopellets made of TPH-acclimated microbes, fermented fruit peel materials, and CaO2 recycled from eggshells were manufactured to treat TPH-contaminated groundwater. The biopellets provided 56 mg of oxygen and achieved a C:N:P ratio by weight of 10:4:1. Moreover, each biopellet was capped with alginate to prolong its floating time in water to 25 days. The mimicked groundwater spiked with 500 mg/L diesel TPHs (TPHd) was treated using our novelly manufactured biopellets. After 8 days of treatment, results showed a 98.8% removal of spiked TPHd at a rate of 64.1 mg/L per day, with a microbial count that increased from nearly zero to 1.0 × 107 CFU/mL. The residual TPHd constituents were mainly C13–C18. Furthermore, microbial consumption of N, P, and oxygen was noted during the 8-day period of TPHd removal. As the TPHd level increased to 1500 mg/L, the removal rate reached 45 mg/L per day, and all TPHd had been removed after 22 days.

The influence of pepper (Capsicum ssp.) as a feed additive to balanced sheep rations on the in vitro digestibility coefficient of nutrients and their fermentation parameters was evaluated in two different inocula: rumen liquid and sheep feces. Four inclusion levels of pepper (0.0%, 0.2%, 0.4% and 0.6% of DM) and two types of inoculum were arranged in a 4 x 2 factorial arrangement, in a completely randomized design. Experimental rations were balanced to include 0.0%; 0.2%; 0.4% and 0.6% Capsicum ssp., with 13.5% crude protein and 70.0% total digestible nutrients (TDN). A basal diet of 60% bulk (corn silage) and 40% of the concentrate containing 0.0% Capsicum ssp. was given to the inoculant donor animals. Two sheep with a mean bodyweight of 27.6 + 1.6 kg were used as inoculum donors (ruminal fluid and feces) for the determination of the IVDC of nutrients. The variables studied were submitted for analysis of variance and the inclusion levels of pepper. Regression analysis was performed at 5% of probability and for the different inocula, a Tukey test was performed at 5% significance. The different levels of inclusion of pepper (0.0%, 0.2%, 0.4%, and 0.6%) in the balanced sheep rations were not altered (P > 0.05) the IVDC of DM; OM; CP and NDF for both inocula (ruminal fluid and sheep feces). However, the use of the different inocula resulted in a change (P < 0.05) in the IVDC value of DM, OM, CP, and NDF, in which the ruminal liquid inoculum presented higher (P < 0.05) values of IVDC for the ration nutrients in relation to sheep feces. The levels of 0, 0%; 0.2%; 0.4% and 0.6% of pepper in ruminant feeds did not change the pH value of the fermented content after a 24-hour in vitro incubation (P > 0.05). However, the use of ruminal liquid as inoculum for the in vitro fermentation of the experimental rations provided a lower value (P < 0.05) for the pH of the fermented content in relation to the sheep feces. The inclusion of this phytogenic additive in ruminant feeds and the use of inoculum based on rumen fluid or sheep feces did not cause changes in the concentration of ammoniacal nitrogen (N-NH3) in the fermented rations after incubation (P > 0.05) for 24 hours in vitro. Thus, the inclusion of up to 0.6% Capsicum ssp. (pepper) in balanced rations for ruminants does not alter the in vitro digestibility coefficient of dry matter, organic matter, crude protein, and neutral detergent fiber, or the in vitro fermentation parameters. However, the use of ruminal fluid as inoculum for the in vitro fermentation presented values of a higher degree of confidence in relation to feces to determine the in vitro digestion coefficient of the nutrients.

Abstract The global pork output in 2018 reached over 100 million tons and will continue to grow. However, great gap still exists between current productivity and the full genetic potential of pigs. Our hypothesis was that current nutritional profile could not meet the pig biological requirement, and that modifying nutritional strategies can maximize their genetic potential. To test this hypothesis, three experiments were conducted using fluid feed, fermented feed, and modified nutrient profile, respectively. Experiment 1 utilized 360 DLY weaning piglets with 6 replicate pens per treatment and 30 pigs per pen. Piglets were fed either a solid feed or a fluid feed with the exact same formulation. Results showed that fluid feed significantly increased ADFI by 53% from 245 to 375 g/d and increased ADG by 54% from 183 to 281 g/d from d 23 to 30 (P &lt; 0.001). Experiment 2 utilized DLY weaning piglets fed 3 dietary treatments: Corn-SBM control, control + 75 mg/kg chlorotetracycline, and 12h-fermented control diet with 6 replicate pens per treatment and 3 pigs per pen. Results showed that 12h fermentation significantly increased ADG from 374 to 474 g/d from 35–56 d, reduced FCR from 1.74 to 1.52, and reduced diarrhea occurrence from 24.4% to 6.6% compared to control (P &lt; 0.015). Performance from the fermentation group was also significantly better than the chlorotetracycline group. Experiment 3 utilized 8 individually-housed PIC pigs fed modified nutrient profile to determine performance from 7 to 147 days of age. Results showed that BW reached 26.5, 33, 100, 112.6 kg on d 60, 70, 134, 147, respectively, and overall FCR was 1.93. Results from these experiments demonstrated that the genetic potential of pig can be realized in greater extend by nutritional strategies.

Mental illnesses and cancer deaths have been alarmingly increased in Thailand over the past decade. There is a search for an alternative remedy from natural sources. This work revealed the probiotic attributes of eighteen microbes from Thai fermented food sources, their neurotransmitter-producing capacities and cytotoxic effects. The probiotic attributes were comprehensively assessed by gelatin hydrolysis, antibiotic susceptibility, auto-aggregation, virulence gene detection, hemolytic activity, hydrophobicity determination, bile and acid tolerance. Neurotransmitters were produced from precursors by microbes over 3 days and analyzed by high-throughput LC-MS/MS. Cytotoxic effects of the selected microbial extracts on MCF-7, HepG2, and HeLa were determined using MTT assay. The results showed that only seven strains; Enterococcus casseliflavus 3.10A1, Enterobacter xiangfangensis 4A-2A3.1, Saccharomyces cerevisiae TC6, Pedicoccus pentosaceus WS11, Lactococcus lactis subspp. lactis TBRC 375, Lactobacillus brevis TBRC 3003 and Bifidobacterium adolescentis TBRC 7154 exhibited promising probiotic potential in every probiotic assessment. Only E. xiangfangensis 4A-2A3.1 and Bacillus spp. PS15 were capable of producing gamma-aminobutyric acid (GABA) from monosodium glutamate (20 mg/mL). GABA production of 4.60 μg/mL over 2 days was found in E. xiangfangensis 4A-2A3.1; however, Bacillus spp. PS15 produced the highest GABA at 5.57 μg/mL on day 1. Moreover, B. adolescentis TBRC 7154 extract showed the lowest IC50 values against MCF-7 (750.02 μg/mL), HepG2 (681.08 μg/mL), and HeLa (425.50 μg/mL) followed by E. xiangfangensis 4A-2A3.1, E. casseliflavus 3.10A1 and P. pentosaceus WS11. These microbes can be used as probiotic supplement with neurodegeneration preventive or chemopreventive potential. © 2021 Friends Science Publishers

Цель данной работы - определить закономерности изменения антиоксидантных свойств обогащенного кисломолочного продукта с повышенной относительной биологической ценностью в процессе хранения. На данном этапе исследования основной задачей являлось определение антирадикальной активности (APA) продукта при хранении в начале и в конце срока годности с учетом коэффициента запаса (1-30-е сут). Кроме того, для выявления влияния термообработки на APA экстракта косточек винограда Vitis Vinifera L. , являющихся обогащающим ингредиентом, при выработке опытных образцов экстракт вносили до и после пастеризации. Полученные данные показали, что продолжительность хранения оказывает значимое влияние на антирадикальную активность продукта, так как она к 30-м суткам снижалась на 30,7-34,2 %. При этом влияние термообработки на АРА вносимого экстракта косточек винограда незначительно. The purpose of this work is to determine the patterns of changes in the antioxidant properties of an enriched fermented milk product with an increased relative biological value during storage. At this stage of the study, the main task was to determine the anti-radical activity (ARA) of the product during storage at the beginning and at the end of the shelf life, taking into account the reserve factor (1-30 days). In addition, to identify the effect of heat treatment on the ARA of grape seed extract Vitis Vinifera L. , which is an enriching ingredient, the extract was added before and after pasteurization during the development of prototypes. The obtained data showed that the duration of storage has a significant effect on the anti-radical activity of the product, since it decreased by 30.7-34.2% by the 30th day, while the effect of heat treatment on the ARA properties of the introduced grape seed extract is insignificant.

Much research on animal feed has focused on finding alternative feed ingredients that can replace conventional ones (e.g., grains and beans) to reduce feed costs. The objective of this study was to evaluate the economic, as well as nutritional value of spent coffee grounds (SCG) and Japanese mugwort (Artemisia princeps) residues (APR) as alternative feed ingredients for ruminants. We also investigated whether pre-fermentation usingLactobacillusspp. was a feasible way to increase the feed value of these by-products. Chemical analyses and anin vitrostudy were conducted for SCG, APR, and their pre-fermented forms. All the experimental diets forin vitroruminal fermentation were formulated to contain a similar composition of crude protein, neutral detergent fiber and total digestible nutrients at 1x maintenance feed intake based on the dairy National Research Council (NRC). The control diet was composed of ryegrass, corn, soybean meal, whereas the treatments consisted of SCG, SCG fermented withLactobacillusspp. (FSCG), APR, and its fermented form (FAPR). The treatment diets replaced 100 g/kg dry matter (DM) of the feed ingredients in the control. Costs were lower for the all treatments, except FAPR, than that of the control. After 24-h incubation, the NDF digestibility of the diets containing SCG and its fermented form were significantly lower than those of the other diets (P< 0.01); pre-fermentation tended to increase NDF digestibility (P= 0.07), especially for APR. Supplementation of SCG significantly decreased total gas production (ml/g DM) after 24-h fermentation in comparison with the control (P< 0.05); however, there were no significant differences between the control and the SCG or the APR diets in total gas production, as expressed per Korean Won (KRW). Diets supplemented with SCG or FSCG tended to have a higher total volatile fatty acid (VFA) concentration, expressed as per KRW, compared with the control (P= 0.06). Conversely, the fermentation process of SCG and APR significantly decreased total gas production and VFA production as expressed per KRW (P< 0.05). Because of their nutrient composition and relatively lower cost, we concluded that SCG and APR could be used as alternative feed sources, replacing conventional feed ingredients. However, pre-fermentation of agricultural by-products, such as SCG and APR, may be inappropriate for improving their nutritive considering the increase in production costs.

A significant number of milk processing companies all over the world confront the challenge of whey recycling. To date, the processing of milk results in a considerable amount of waste products, which can be used in core production processes, for the output of additional products and can help neutralize harmful effects of milk processing waste waters on the environment. This factor emphasizes the relationship between food and environment. The extraction of water from cheese and curd whey in the manufacture of concentrates gives a solution to one of the challenges facing the dairy industry – the processing of secondary dairy raw resources. Whey concentrates have certain advantages because they are long-shelf-life and easy-to-transport products of high nutritional and biological value. Several studies have assessed process flow factors of whey dehydrating in the manufacture of whey concentrates. The research was carried out using an experimental unit of a vacuum dryer with the thermal radiation power supply. The concentrates with a percentage of dry substances ranging from 11 to 30 % were produced. The important outcomes to emerge from the study are data on the dynamics of vacuum-induced water extraction from cheese and curd whey, its appropriate modes, as well as regularities and specifics of water removal. The quality of concentrates was assessed according to key parameters. The organoleptic properties of sample concentrates met all criteria quite sufficiently, being similar to input parameters. Whey concentrates with a mass fraction of dry substances from 11 to 30.0 % are recommended to use in recipe compositions of fresh and fermented whey-containing drinks. IR-rays represent a good alternative to conventional thickening and drying processes of raw materials at high processing temperatures. The properties of input raw materials remain unaffected owing to non-destructive temperature modes of the vacuum dryer.

Cheeses produced using traditional technology usually contains 45 % 50 % of milk fat in dry matter, therefore they are classified as high-calorie foods and are not recommended to be included in the diet of people who need dietary nutrition. In the European countries, dietary low-calorie rennet cheeses are widely represented in the trade network, and in Ukraine such products are quite rare, expensive and usually of foreign production. Milk fat is an important structural and flavoring component of rennet cheeses and a significant decrease in its content in milk leads to a product of low consumer quality with problems such as unexpressed taste, coarse, rubbery consistency. In proportion to the decrease in the mass fraction of fat in the cheese, the disadvantages are more pronounced. The solution to this problem requires an integrated approach, which consists in the correction of technological methods for the production and maturation of cheese and the optimization of the composition of fermented microflora. It is possible to improve the sensorial characteristics of low-calorie cheeses by introducing into their structure exopolysaccharides (EPS) – high-molecular polymers synthesized by some strains of lactic acid bacteria (LAB). Research subject: biological and technological properties of LAB. The aim of this work is to search for strains of LAB with given physiological and biochemical characteristics for further use in the production of dietary rennet cheeses with a reduced mass fraction (MF) of fat. The common microbiological and technological research methods were used in the work. Research results. The expediency of using sucrose as the only source of carbohydrate nutrition for screening LAB with EPS-activity has been shown. Six strains of LAB identified as Streptococcus thermophilus, Lactobacillus bulgaricus, Lactobacillus plantarum and Leuconostoc mesenteroides were withdrawn from non-commercial fermented milk products and studied for their technological properties. In laboratory conditions, the expediency of using LAB with EPS-activity for the production of cheese with a reduced MF of fat has been confirmed. The use of new cultures LAB in the composition of the starter culture allowed increasing the moisture content in the cheese by (6.5 8.3)% compared to the control and to improve its texture and taste. Scope of research results application. The use of LAB-producers of EPS will improve the consumer characteristics of rennet cheeses with reduced fat content, expand the domestic range of these products, increase their biological value, will have a positive effect on the health of people who need dietary nutrition, as well as all population groups.

Cocoa pod husk is abundant as a waste product of cocoa plantation and potential as feed ingredient but has low nutritional value. To increase the nutritive value of cocoa pod husk (CPH), biological process through solid substrate fermentation using <em>Aspergillus oryzae </em>and <em>Aspergillus niger </em>and addition of two doses (N1 and N2) of nitrogen mixture had been done. The product is Fermented Cocoa Pod Husk (FCPH). Protein content increased from 50 g/kg before fermentation to 133.8 g. kg^-1 for N1 for <em>A. niger</em> and 150 g/kg using <em>A. oryzae</em>. True protein were 99.8 and 93.5 g/kg for N1 and N2 treatments (<em>A. niger</em>); 119 and 104.1 g/kg for N1 and N2 treatments (<em>A. oryzae</em>). <em>Aspergillus niger</em> showed a superiority in term of enzymes production when compared to <em>Aspergillus oryzae</em>. Mannanase activy in <em>A. niger</em> fermentation product with N1 dose reached up to 2654 U/g and <em>A.oryzae</em> was 1122 U/g. Dry matter and protein digestibility for <em>A. niger</em> FCPH were 47 and 57% and <em>A. oryzae</em> FCPH were 52 and 62% repectively. Fermentation processed of CPH yield a product that very potential as an alternative feed with higher in protein content and contain mannanase enzyme.

Thirty-two Large White × Landrace male pigs were used to determine the relationships between the rates of tissue deposition and age, in boars and barrows under 2 housing systems. A 2 × 2 factorial design was used, with the respective treatments being sex (boar or barrow) and housing condition (individually penned system or group-penned system). Surgical castration was performed at 7 days of age. Individually housed pigs were used to provide an estimate of potential growth and were weaned at 10 days into individual cages and provided with supplemental fermented skim milk for 2 weeks. Group-housed pigs were weaned at 24 days of age and reared in group pens of boars and castrates typical for commercial production. Conventional weaner, grower, and finisher diets were provided ad libitum to all pigs from weaning onwards. A Hologic QDR4500A dual-energy X-ray absorptiometer (DXA) was used to determine lean, fat, and bone composition at 4-weekly intervals from 10 until 150 days of age. Over the 20 weeks of the study, boars deposited less fat than barrows (136 v. 179 g/day, s.e.d. = 6.49, P < 0.001). Over the same time frame, boars deposited more lean tissue than barrows when individually penned (490 v. 444 g/day, s.e.d. = 14.4, P < 0.05) but there was no difference in group-penned pigs (464 v. 443 g/day, s.e.d. = 14.4, P > 0.1). This was more pronounced in the final 4 weeks of growth when individually penned boars deposited 200 g/day more lean than barrows (P < 0.001), with no increase in group-penned animals. However, during this growth phase, group penning further increased the fat deposition margin between boars and barrows where boars deposited 90 g/day less fat when individually penned (P < 0.001), but 140 g/day less fat (P < 0.001) when group penned. The results show that the advantages of boars in terms of growth and lean tissue composition are substantially reduced in group-penned situations. However, because of fat deposition, boars retain some advantage over barrows in group-penned systems at liveweights over about 50 kg. These data may aid in the accurate prediction of the nutrient requirements and optimum slaughter weight for barrows.

Abstract Objectives Q CAN PLUS® is a fermented soy powder currently marketed in the US as a food product. We conducted a feeding trial to examine the effects of Q CAN PLUS® on the nutrient composition of the habitual diets of individuals at high risk of cardiovascular diseases. We hypothesized that the nutrient profile, particularly nutrients associated with CVD risk, differs among individuals in the Q CAN PLUS® phase compared to the placebo phase. Methods Study design was a randomized, controlled crossover intervention with twenty-four free living adults (29–75 years old; 80% female; 8% normal BMI, 45% overweight, 47% obese) at high risk of cardiovascular diseases. Subjects were randomized to receive either Q CAN PLUS® (fermented soy) powder or placebo powder for twelve weeks and switched over to the placebo treatment after two weeks of wash out period. Two 24-hour dietary recalls were collected during each phase which included one weekday and one weekend dietary recall. The nutrient consumption per day (mean ± SD) were based on a synthetic week by using the following formula: ((weekday * 5) +(weekday * 2))/7. Results On average, intake of cholesterol (211 ± 169 mg) was 13% lower (P = 0.05) in the Q CAN PLUS® phase compared to placebo (243 ± 179 mg). Total soy isoflavones (444 ± 210 mg) was 12 times higher (P &lt; 0.0001) during Q CAN PLUS® phase than the placebo phase (33 ± 93 mg). Dietary total carbohydrate, total protein, vegetable protein, animal protein during Q CAN PLUS® phase were not statistically different from placebo phase. Conclusions The differences in the intake of cholesterol and total soy isoflavones may have implications on risk of cardiovascular diseases. Funding Sources BESO Biological Research Inc Diamond Bar, CA, USA.

Biogas production considered the most encouraging sources of renewable energy in Sudan. Anaerobic process of digestion is considered as efficient techniques of producing biogas. The process also a trustworthy method for treatment of municipal wastes, and the digested discharge could be utilized as soil conditioner to improve the productivity. This research work states at the option of using domestic sludge of the wastewater treatment plant in Soba municipal station (south of Khartoum-Sudan) to produce biological gas (biogas). A laboratory investigation was carried out using five-liter bioreactor to generate biogas for 30 days. The total volume of gas made was 270.25 Nml with a yield of 20 Nml of biogas/mg of COD removed. Chemical oxygen demand, Biological oxygen demand, & total solids drop produced were 89, 91 & 88.23% respectively. Microbial activity was declined from 1.8x107 (before starting the process of digestion) to 1.1x105 germs/mL (after completion of 30 days of digestion). This study offered a significant energetic opportunity by estimated the power production to 35 KWh.Key word: Sludge, municipal plant, organic material, anaerobic process, breakdown, biological gas potentialNTRODUCTIONIncreasing of urban industries style in the world has given rise to the production of effluents in huge amounts with abundant organic materials, which if handled properly, be able to end in a substantial source of energy. Although of a fact that there is an undesirable environmental effect related with industrialization, the influence can be diminished and energy can be tapped by means of anaerobic digestion of the wastewater (Deshpande et al., 2012). Biological wastewater treatment plant (WWTP) is a station for removal of mainly organic pollution from wastewaters. Organic materials are partly transformed into sludge that, with the use of up-to-date technologies, represents an important energy source. Chemical biological, and physical technology applied throughout handling of wastewater produce sludge as a by-product. Recent day-to-day totals, dry solids range from 60–90 g per population equivalent, i.e. EU produces per year 10 million tons of dry sludge (Bodík et al., 2011). Sludge disposal (fertilizers use, incineration, and landfills) is often explored since of increasingly limiting environmental legislation (Fytili and Zabaniotou, 2008). The energy present in sludge is obviously consumed in anaerobic digestion. Anaerobic Process is considering the most appropriate choice for the handling of organic effluents of strong content. This process upgraded in the last few years significantly with the applications of differently configured high rate treatment processes, particularly for the dealing of industrial releases (Bolzonella et al., 2005). Anaerobic process leads to the creation of biological gas with high content of methane, which can be recovered, and used as an energy source, making it a great energy saver. The produced gas volume during the breakdown process can oscillate over a wide range varying from 0.5 – 0.9 m3 kg–1 VS degraded (for waste activated sludge) (Bolzonella et al., 2005). This range rest on the concentration of volatile solids in the sludge nourish and the biological action in the anaerobic breakdown process. The residue after digestion process is stable, odorless, and free from the main portion of the pathogenic microorganism and finally be able to use as an organic nourishment for different application in agriculture. Sludge significant coming out from breakdown which allows to yield a renewable energy, that was cheap, obtainable, & no polluting. Sustainable development considered the production of biogas as environmentally friendly and an economic key (Poh and Chong, 2009).OBJECTIVES Sudan have huge tones of sewage sludge from domestic sewage water is accumulated daily in lagoon of soba sewage treatment plant, so this work, we were carried for energy production and treatment of sludge, which constitutes a plentiful waste which ever know any sort of handling after few years from establishing the station.MATERIALS AND METHODSExperimental apparatus: Anaerobic breakdown was done in five liters fermenter. The fermenter was maintained at 35oC in a thermostatic bath and stirred regularly. U shaped glass tube was connected to the fermenter, allowing the measurement of produced biogas volume and pressure. Water displacement technique was used for determination of the volume of produced biological gas (biogas) at the beginning of each sampling. Testing of the biogas combustibility was determined by connecting one of ends of the tube to a gas collection and storage device (balloon), the other end to a Bunsen burner. In the process of reduction of carbon dioxide (CO2) to maximum dissolution in the tube the liquid must be a salty saturated acid solution (5% citric acid, 20% NaCl, pH ¼ 2) (Connaughton et al., 2006).Substrate: About 5L sludge containing culture medium were taken from the lowest part of the first settling tank in Soba station. The moisture content of initial substrate was 35%. The collected sample was preserved at 4oC prior to loading the biological reactor (Tomei et al., 2008). Table 1 showed the sludge features in the reactor with a loading rate of 16 g TS/L, (Connaughton et al., 2006; Tomei et al., 2008).Analytical Methods: The pH was controlled by using HANNA HI 8314 model as pH meter device. Assay was used for determination of Alkanility & Volatile fatty acids (Kalloum et al., 2011). The standard method of analysis was used for recognized the Chemical Oxygen Demand (COD) (Raposo et al., 2009). Titrimetric method was used for analyzing Volatile fatty acids (VFA). Alkalinity assay was used for determination of Total Alkalinity (TA). Oxitop assay was used for measuring the biological oxygen demand. Ignition method was used for measuring Volatile Solids (VS) by losing weight in dry sample at 550oC in the furnace, & Total solids were done to constant weight at 104oC (Monou et al., 2009). A method of water displacement was used for determination of the total volume of Biological gas produced (Moletta, 2005). Microbial species & analyses were determined by microbial standard assay. Sample analysis was done by explore of three replicates and the outcomes were the middling of these replicates. Startup of experiments continues until a bubble of gas was detected.RESULTS AND DISCUSSIONMeasurement of pH: Figure 2 exhibited pH trends during 30 days with a drop pattern from 7.0 to 6.0 during the first five days; this was mainly because of the breakdown of organic materials and the development of (VFA). Then later, an increasing pattern in pH was noticed to 6.98, for the next week, then Steadying around this pH level was continued till the completion of the breakdown period which taken 30 days. Those out comes were also reported by other researchers (Raposo et al., 2008)Measurement of VFA: Development of VFA throughout 30 days was depicted in figure 3, an increase in volatile fatty acids up to 1400 mill equivalents per liter (meq/L) in the first ten days. This criterion of making of volatile fatty acid is typical to the researcher’s report of identification of hydrolysis in acidogenesis stage (Parawira et al., 2006). The decline in volatile fatty acids after the tenth day was owing to intake by bacteria which would relate to the stage of acetogenesis.Total alkalinity (TA): During the ten days, we observed rise in volatile fatty acids content followed by a drop in a pH in the same time (figures 4 and 5). Encountered to these alterations, an increase in the total alkalinity in the medium for reestablishing situations of alkalinity to the outbreak of methanogens stage (figure 4). Through all the digestion period the ratio of VFA/TA which was equal and lower than 0.6±0.1 were described in figure 6. These ratios designated the achievability of the procedure despite the essential production of volatile fatty acid (Chen and Huang, 2006; Nordberg et al., 2007). The anaerobic digestion process may be hinder by the production of volatile fatty acid.Biogas production: Pressure measurement and biogas volume were used for controlling biogas production. Figure 7 explained the changing in biogas pressure throughout the digestion period. quality of Biogas was obtained with minimum methane of 40% (Bougrier et al., 2005; Lefebvre et al., 2006). Total volume of biological gas production was 270.25 Nml. The yield of biological gas was 20.25 Nml/mg COD removed, which is in range of the others researcher report (Tomei et al., 2008). Biogas production can be calculated from the following formula (Álvarez et al., 2006): Biogas production= (Total quantity of biogas produced)/(Total solid).The COD and BOD removal: Chemical oxygen Demand (COD) and Biological Oxygen Demand (BOD) showed a significant reduction of 89% and 91% respectively (figures 8 and 9). Consequently these reduction in contaminants proved that anaerobic process of digestion was an operational technique for removal of organic pollution. Some researchers reported the same results (Bolzonella et al., 2005; Álvarez et al., 2006; Wang et al., 2006). Another criterion for proving the removal of organic pollutants was reduction of total solids (TS), where the drop approached 88.23% (figure 10). Some researcher’s reports approached the same drop (Hutnan et al., 2006; Linke, 2006; Raposo et al., 2009). Therefore it was possible to conclude that anaerobic digestion necessary showed decrease or reduction of organic pollutants rates because of the transformation of organic substances into biogas and accordingly led to the drop of chemical oxygen demand (COD). This could be explained in figure 11 by the comparison of the two techniques during the anaerobic digestion process. That means the chemical oxygen demand (COD) drop should be tailed essentially by Total solids drop (TS).Microbial activity: Figure 11 showed the microbial variation during anaerobic digestion. The total micro flora (total germs) declined from 1.8x107 (before starting the process of digestion) to1.1x105 germs/mL (after completion of 30 days of digestion). Moreover figure 12 obviously explained what was running during the process of digestion in the reactor, microbial species vanishing after the 30 days such as streptococci and Escherichia coli. Some researchers reports explained that there was some sort of relationship between physicochemical and the biological parameters of micro flora with total solid (TS). figure 13 described obviously this relationship of the drop of micro flora which go along with total solids reduction. This intended that consumption and a declining in the mass residue of organic materials created at the termination of digestion was the outcome of the transformation of organic materials into biological gas and also the sum of microorganism reduction. This attained result proved that the process of anaerobic digestion was a good process for decontamination (Deng et al., 2006; Perez et al., 2006; Davidsson et al., 2007).CONCLUSIONSoba sludge’s municipal station carried in this research paper demonstrated operative for biological gas production (biogas). During the first five days, breakdown of organic materials and the formation of volatile acids were started. Volatile fatty acids increased up to 1400 mill equivalents per liter (meq/L) in the first ten days, then started to decline in after the tenth day this owing to intake by bacteria which would resemble to acetogenesis stage. The biogas production lasted until the 21th day then starting decreasing till the last day (30 day) this due to instability of the culture medium of fermentation which became completely poor. COD and BOD showed a significant reduction of 89% and 91% respectively. Another criteria for proving of removal rate of organic pollutants was reduction of total solids (TS), where the reduction rate approached 88.23%. Total volume of biological gas production was 270.25 Nml. The yield of biological gas was 20.25 Nml/mg COD removed, which is in range of the others researcher report. The total micro flora (total germs) declined from 1.8x107 (before starting the process of digestion) to 1.1x105 germs/mL (after completion of 30 days of digestion). Study proved that process of anaerobic digestion was a good process for decontamination. Industries and will be usefulness for bioremediation in marine environment and petroleum industry.ACKNOWLEDGMENTSThe authors wish to express their appreciation to Soba treatment plant, for their financial support of this research.CONFLICT OF INTERESTThe authors wish to express their appreciation to Soba treatment plant, for their financial support of this research.REFERENCES Álvarez, J., I. Ruiz, M. Gómez, J. Presas and M. 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Kombucha is a fermented tea beverage prepared as a result of the symbiotic nature of bacterial cultures and yeast, the so-called SCOBY (Symbiotic Cultures of Bacteria and Yeasts). Kombucha is characterised by rich chemical content and healthy properties. It includes organic acids, minerals and vitamins originating mainly from tea, amino acids, and biologically active compounds—polyphenols in particular. Kombucha is prepared mainly in the form of black tea, but other tea types are increasingly often used as well, which can significantly impact its content and health benefits. This work shows that the type of tea has a significant influence on the parameters associated with the antioxidant potential, pH, as well as the content of acetic acid, alcohol or sugar. Red tea and green tea on the 1st and 14th day of fermentation are a particularly prominent source of antioxidants, especially polyphenols, including flavonoids. Therefore, the choice of other tea types than the traditionally used black tea and the subjection of these tea types to fermentation seems to be beneficial in terms of the healthy properties of kombucha.

Whole-crop cereal silage represents an important component of ruminant diets and is used as a substrate for biogas production. Due to the scarcity of data on whole-crop rye (Secale cereale L., WCR), our study aimed to evaluate the effects of a range of biological and chemical additives of different compositions on the fermentation and aerobic stability of silage made from this species. In addition, the production of various volatile organic compounds (VOCs), which potentially contribute to greenhouse gas emissions, was monitored. Regardless of additive treatment, all WCR silages were well fermented as reflected by the complete absence of butyric acid. Inoculants containing Lactobacillus buchneri and chemical additives reduced dry matter (DM) losses during fermentation for 53 days (p < 0.001), which were closely related with the concentration of ethanol upon silo opening (R2 = 0.88, p < 0.001). Silage treated with Lactobacillus buchneri, alone or in combination with a homofermentative strain, had the lowest yeast count (p < 0.001) and, simultaneously, the highest aerobic stability (p < 0.001). Chemical additives outperformed all other additives by largely restricting the formation of ethyl esters of lactic and acetic acids (p < 0.001). The concentration of ethanol strongly correlated with those of ethyl lactate (R2 = 0.94, p < 0.001), ethyl acetate (R2 = 0.85, p < 0.001), and total ethyl esters (R2 = 0.94, p < 0.001). The use of a simple linear regression model exclusively based on the ethanol content proved useful to predict the concentration of total ethyl esters in WCR silage (R2 = 0.93, p < 0.001).

The ?Pirot ?ironed? sausage? (Pis) is a traditional, dry, fermented product originating from the town of Pirot, situated in southeastern Serbia. This product is made from different types of meat (goat, sheep, beef and donkey) and spices, without additives or starter cultures. Pis is an organic, unprocessed product, without heat or smoke treatment. The aim of this study was to characterize 120 isolates of lactic acid bacteria (LAB) from Pis produced by six different brands during a two-year period using phenotypic and genetic identification. Preliminary characterization of the LAB was based on general morphology and biochemical tests. Repetitive elements such as REP, BOX and GTG5, found in the genome of these bacteria, and randomly amplified polymorphic DNA-polymerase chain reaction (RAPD PCR) sequences were used for determination of genetic polymorphism. Identification by 16S rRNA gene sequencing showed the presence of only two LAB species, Lactobacillus sakei (76%) and Leuconostoc mesenteroides (24%). However, genetic polymorphism was detected using fingerprinting methods. In comparison with other primers, the profiles obtained with GTG5 showed the highest heterogeneity for most of the tested isolates, with sequencing results additionally confirming its discriminatory power. In addition, M13 RAPD primer also produced satisfactory separation of the tested isolates.

Citric acid is an organic acid that has a wide range of applications in food industry and other industries. In the pharmaceutical industry, citric acid is used to produce different metal citrate salts so that these minerals can be biologically used in many medicines. For example, iron citrate made from citric acid provides iron to protect human blood, or is used to produce medicines in tablet or cream forms and cosmetics. In this research, we investigated effects of technological factors on the fermentation process of citric acid in the solid state using sugarcane bagasse and Aspergillus Niger. We conducted an experiment to determine the content of citric acid obtained in fermented liquid using HPLC. The result showed that substrate humidity, culture ratio and duration significantly influence on the fermentation process. Through the investigation of water content change in solid state from sugarcane bagasse, we indicated that during the fermentation time, a fixed amount of water to be supplemented every day to maintain the initial humidity. Based on the results, we recommend a fermentation process of citric acid using sugarcane bagasse under the following condition: substrate humidity 80%, temperature 30OC, 9-day duration, A.niger ratio 10% (v/w). The content of citric acid that can be obtained under the above condition is 12.02g/100g of sugarcane bagasse.

The aim of this study was to identify and characterize the lactic acid bacteria (LAB) of artisanal Golija raw and cooked cows? milk cheeses traditionally manufactured without the addition of starter culture. A total of 188 Gram-positive and catalase-negative isolates of Golija cheeses were obtained from seven samples of different ripening time. Phenotypebased assays as well as rep-PCR and 16S rDNA sequence analysis were undertaken for all 188 Lstrains. The most diverse species were isolated from 20-day-old BGGO8 cheese (Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus casei/paracasei, Lactobacillus sucicola, Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. lactis bv. diacetylactis, Enterococcus faecium, Enterococcus durans and Leuconostoc mesenteroides). In other Golija cheeses Lactobacillus reuteri, Lactobacillus curvatus, Lactobacillus rhamnosus, Lactococcus lactis subsp. cremoris, Lactococcus garvieae, Streptococcus thermophilus and Leuconostoc pseudomesenteroides were found. Pronounced antimicrobial properties showed enterococci (13/42) and lactococci (12/31), while the good proteolytic activity demonstrated lactococci (13/31) and lactobacilli (10/29).

<p>Abstract<br />Organic fertilizer is highly recommended for soil and plant because it can improve the productivity and repair physical, chemical, and biological of soil. Sargassum sp. and surimi liquid wastes contain organic matter and nutrient needed by plants and soils. The addition of marine bio-activator which contains bacterial isolates from litter mangrove serves to accelerate the composting time and increases the activity of microorganisms in the decomposition process. The purpose of this study was to determine optimum time and the best formulation of decomposition process organic fertilizer. Raw materials used a waste of seaweed Sargassum sp., marine bio-activator and surimi liquid waste from catfish (Clarias sp.). The research was conducted six treatments control, Sargassum sp. + marine bio-activator, surimi liquid waste , Sargassum sp. + marine bio-activator + surimi liquid waste 80%, 90%, 100%. All treatments were fermented for 9 days and analysed the C-organic, total N, C/N ratio, P2O5, K2O on days 0, 3, 6 and 9. The results showed the optimum fermentation period was on the 6th day. The most optimum concentration of surimi liquid waste added was at a concentration of 90%, with characteristics of the products was C-organic 0.803±0.0115%, total N 740.063±0.0862 ppm, C/N ratio 10.855±0.1562, P2O5 425.603±0.2329 ppm, K2O 2738.627±0.2836 ppm.</p>

Lactobacilli are widely used in clinical practice as probiotics, biologically active additives and probiotic products for functional nutrition. Some probiotics can be considered as bacterial vaccines due to induction of immune response, accompanied by production of specific antibodies. The aim of the present study was to evaluate the state of cellular and humoral immunity in women by using probiotic strains of lactobacilli. The study included 31 healthy women aged 25-45 years. As a source of probiotic lactobacterial complex, we used the “Provag” preparation (RU 77.99.11.003.E.003746.02.11 of 11.02.2011, 1 capsule contains 109 Lactobacillus gasseri 57C, Lactobacillus fermentum 57A и Lactobacillus plantarum 57B). The drug was used for 30 days, at a rate of one capsule per day. The immune system was examined twice: before administering the drug and after 30 days of treatment. The study of blood lymphocyte populations and subpopulations was performed by flow cytometry using direct immunofluorescence technique. The concentration of IgA, IgM, IgG in blood serum was determined using enzyme immunoassay. To determine specific antibodies, we used passive hemagglutination reaction with erythrocyte diagnosticum. The complex of probiotic lactobacilli Lactobacillus gasseri, Lactobacillus fermentum and Lactobacillus plantarum corresponding to the “Provag” preparation was used as a source of antigen. It has been revealed that the number of T and B lymphocytes in peripheral blood increased after 30 days of treatment with the probiotic preparation “Provag” in healthy women. Elevated contents of T cells was due to the T helper cell fraction. Increased levels of T helpers and B lymphocytes were associated with stimulation of humoral immunity, as evidenced by increasing concentration of IgA and IgG in blood serum. By means of passive hemagglutination reaction, we have found that 90% of healthy women showed increased concentrations of specific IgA in blood after 30 days of treatment with “Provag” preparation.