Journal articles on the topic 'Du shu fang fa'

??? Yehang Chuan ‘Night Ferry’ is an encyclopaedic masterpiece and the chef-d’oeuvre of ?? Zhang Dai (circa 1597-1689), an illustrious historian, poet, dramatist, essayist, aesthete, musician and gastronomist in late Ming and early Qing China. Night Ferry cumulates more than four thousand entries and encompasses a veritable cornucopia of topics in an elephantine range. In this research, I investigate Chapter Twenty ?? Fang Shu ‘Alchemy and Sorcery’ of Night Ferry, which comprises Section ?? Fu Zhou ‘Amulets and Incantations’ and Section ?? Fang Fa ‘Prescriptions and Practices’. Both sections abound with depictions pertaining to occult acts and paranormal forces, the vast majority of which embody cultural allusions concerning religion, divination and patriarchy. Furthermore, Chapter ‘Alchemy and Sorcery’ is featured by humorousness, though Night Ferry is not a dedicated jestbook. The humorous effect in Night Ferry is not attained via sarcasm or homo-/hetero-erotism, as manifested by derisive and prurient jokes compiled in a renowned pre-modern jestbook entitled ????Xiao Lin Guang Ji ‘A Collection of Classic Chinese Jokes’.

Abstract Fanconi anemia (FA) is a genetic disorder characterized by bone marrow failure, a predisposition to cancer, congenital abnormalities and a cellular hypersensitivity to DNA interstrand cross-linking agents. This hypersensitivity in FA cells correlates with a defect in ability to repair interstrand cross-links. We have shown that the structural protein, nonerythroid a spectrin (aIISp), plays an important role in the repair of DNA interstrand cross-links and that there is a deficiency in aIISp in FA cells. The reduced levels of aIISp in FA cells are due to reduced stability of this protein. We propose that the stability of aIISp is dependent upon one or more of the FA proteins. The present study was undertaken in order to get a clearer understanding of the proposed role of FA proteins in maintaining the stability of aIISp in the cell and the functional importance of this relationship in the repair of DNA interstrand cross-links and the repair defect in FA cells. For these studies, FA proteins were examined for their ability to directly interact with aIISp and, if so, to map the sites of interaction. Four overlapping regions of aIISp were constructed and binding of FA proteins to each of these regions was examined using yeast two-hybrid analysis. Of the proteins examined, FANCG was found to interact with one of these regions of aIISp and specifically with the SH3 domain within this region. The site of interaction in FANCG was mapped to a SH3 domain binding motif, which contains a consensus sequence with preference for the SH3 domain of aIISp. The sites of interaction in both FANCG and aIISp were confirmed using site-directed mutagenesis. Two FA proteins that did not contain any SH3 binding motifs, FANCC and FANCF, did not interact with the SH3 domain of aIISp or any of the other regions of aIISp. These results thus demonstrate that one of the FA proteins, FANCG, contains a class of motifs that has specificity for binding to SH3 domains and binds to the SH3 domain of aIISp via this motif. This binding is important in the DNA repair process and in the FA repair defect, as is shown by our in vivo studies in which FA-G cells, transformed with the FANCG cDNA and stably expressing FANCG, showed normal levels of aIISp and a correction of the defect in ability to repair DNA interstrand cross-links. We therefore propose that the binding of FANCG to aIISp is not only important for the stability of aIISp in cells but also for the role aIISp plays in the DNA repair process. Thus a deficiency in FANCG, such as occurs in FA-G cells, could lead to reduced stability of aIISp which in turn could be an important factor in the defective DNA repair pathway in FA cells.

This study is an in-depth analysis of the Chinese-style work of modern Chinese lyricist Fang Wen shan. Fang Wen shan's work has been evaluated as a lyric containing ancient elements, so the characteristics of creation and literary elements contained in the lyrics of the song “Fa Ru Xue” were studied. To this end, in order to analyze the characteristics of the rhyme, artistic conception, and rhetoric of the notice contained in the lyrics, Fang When san’s creative career was investigated, and previous studies on Fang Wen shan’s work were found. Through prior research on Fang Wen shan’s work, the keyword and literary value of the song were explored, and the positive effects of Fang Wen shan’s Chinese-style work on the development of the Chinese music industry were explored through creative characteristics and literary value analysis of the lyrics.

The account given of the Bamboo Annals pre-Conquest chronology in Part 1 of this paper bears comparison with the internally consistent chronological sequence found in Yi Zhou shu, whose coherence has not been fully appreciated by those attempting to reconstruct the chronology. As we saw in the discussion of King Wu's reign in Part 1, Yi Zhou shu ‘Da kuang’ and ‘Wen zheng’ chapters are correct in dating the appointment of King Wu' s siblings as viceroys in the former Shang domain, which event we know took place in the Conquest year, to the 13th year of the Mandate, a finding that is fundamentally incompatible with Chou Fa-kao's, David Nivison' s, and Edward Shaughnessy' s account. The same two chapters in combination with ‘Zuo luo’ are also correct in implying that King Wu died in the 14th year. Furthermore, ‘Wen zhuan’ chapter of Yi Zhou shu correctly dates King Wen' s demise to the ‘9th year of the Mandate’ in those very words.

Objectives. Little information is available regarding the use of Chinese herbal medicine to treat mastitis. This study evaluated the prescription patterns of Chinese herbal medicine products in women with mastitis in Taiwan. Design. This is a population-based cross-sectional study. Setting. 8,531 women aged 20–49 years, who received a diagnosis of mastitis between 2004 and 2013, were identified from the Longitudinal Health Insurance Database in Taiwan. We collected data on demographic characteristics, including age, monthly insurance premium, and urbanization level. The ten most Chinese herbal medicines prescribed for mastitis were assessed, including frequency, percentage, average daily dose, and average duration of prescription. Main outcome measures. We analysed the ten most single Chinese herbs and Chinese herbal formulae prescribed for mastitis. Results. Overall, 437 (5.1%) women received Chinese herbal medicine to treat mastitis. Mai Men Dong (Ophiopogon japonicus (Thunb.) Ker Gawl.; 22.3%), Pu Gong Yin (Taraxacum mongolicum Hand.-Mazz.; 7.8%), and Wang Bu Liu Xing (Vaccaria hispanica (Mill.) Rauschert; 3.5%) were three of the most commonly prescribed single Chinese herbs for mastitis. Xian-Fang-Huo-Ming-Yin (18.2%), Jia-Wei-Xiao-Yao-San (9.1%), and Chai-Hu-Shu-Gan-San (8.4%) were three of the most commonly prescribed Chinese herbal formulae. Conclusion. Xian-Fang-Huo-Ming-Yin can clear heat, detoxify body, alleviate swelling, activate blood, and relieve pain. It was the most frequently prescribed Chinese herbal formula in patients with mastitis.

AbstractBan Gu's compilation of the Han shu may be seen in the context of a number of intellectual and religious developments. By his time the idea of the Tian ming and the theory of the Wu xing were being applied to imperial times. Officials were quoting the sayings of Kongzi to support their arguments, and the writings of distinguished scholars such as Jing Fang, Liu Xiang, Liu Xin and Yang Xiong were well known. The religious controversies that had begun in the reign of Chengdi had died down. The pursuit of scholarship had received a new impetus thanks partly to the discussions held in 79 ce. Ban Gu drew somewhat freely on existing literature, being prudent to select material that would not arouse enmity; his sister called on official documents to complete her part of the history. As an innovator Ban Gu introduced chapters on subjects that had not been treated in the Shi ji, such as bibliography and the laws. Ready to criticize the actions of officials or the character of an emperor openly, he also contrived to do so implicitly.

This study examined how Taiwanese philosophy has been received and researched in South Korea since its start to the present day. It takes the form of a survey, classifying the articles about Taiwanese philosophy which were published in South Korea over the years from 1994 to 2018 by the theme. It selected nine philosophers whose influence was profound in Taiwanese philosophy and observed the currents in the scholarship on each philosopher. The names of the selected philosophers are: Fang Thomé H., Hu Shi, Huang Chun-chieh, Lin Yutang, Liu Shuxian (Liu Shu-hsien), Mou Zongsan, Tang Junyi (Tang Chun-I), Xu Fuguan, Yu Yingshi (Yu Ying-shih). Sixty-one related papers were summarized and reviewed, and each of them was classified by the publication date, author, language, publisher and keywords. The survey revealed the limitations in Asian philosophy scholarship with regard to Taiwanese philosophy in South Korea, in terms of both quantity and quality. The survey also suggested a possible solution to these limitations and directions for scholars in the future. The study thus serves as a foundation that can boost discussion and the balanced development of South Korean philosophy studies, as well as of Asian philosophy in general.

Statement of the problem. The twentieth century marked an increased interest in polyphonic music. The geography of polyphonic works for piano expanded significantly and a creative development of many Chinese composers, writing polyphonic piano pieces, took place. Today, polyphonic pieces make up a significant part of the piano repertoire in China, but they are little studied by musicologists and performers. The objective of this study – to reveal the contribution of Chinese composers to the creation of polyphonic piano repertoire of the XX – early XXI century. Analysis of the research and publications on the theme. А large number of modern authors study polyphony from the point of physical and mathematical research methods (Igarashi, Yu. & Ito, Masashi & Ito, Akinori, 2013; Weiwei, Zhang & Zhe, Chen, & Fuliang, Yin, 2016; Li, Xiaoquan et al. others, 2018). This approach does not reveal the factual musical component of polyphonic genres. In the 20th century, musicologists explored polyphony in musical folklore (Wiant, 1936; Fan Zuyin, 2004; Li Hong, 2015) and in professional Chinese composing (Sun Wei-bo, 2006, Winzenburg, 2018). The scientific novelty. This article studies the role of Chinese composers in the development of the world polyphonic piano repertoire of the XX – early XXI century. The methodological basis for the analysis of polyphonic works was the theoretical concepts of P. Hindemith, Peng Cheng, Fang Zuin, Li Hong, Sun Wei-bo. The results of the study. The research outcomes demonstrate the evolutionary development of the genre diversity of Chinese piano polyphony as well as those composers who created magnificent musical pieces. Conclusions. Chinese composers have fully mastered the art of modern counterpoint, represented by the genres of polyphonic program pieces (He Lu Ting), invention (Xiao Shu Xian, Du Qian, Sun Yun Yin, Chen Chen Quang), polyphonic suite (Ma Gui), large polyphonic cycle ( He Shao, Chen Hua Do, Xiao Shu Xian), fugue (Li Jun Yong, Yu Su Yan, Chen Gang, Tian Lei Lei, Duan Ping Tai, Zheng Zhong, Xiao Shu Xian) and small cycle “Prelude and Fugue” (Ding Shan Te, Chen Zhi Ming, Wang Li Shan). Creatively assimilating and rethinking the experience of Western polyphonists, Chinese composers have filled their polyphonic works with national features, firmly linking them with the origins of Chinese traditional and folk music. The polyphonic way of transmitting musical material becomes the most expressive at the moments of profound creativity and musical dramatization.

Shakespeare studies in Mainland China and Taiwan evolved from the same origin during the two centuries after Shakespeare being introduced into China in the early nineteenth century. Although Shakespeare was first seen on the Taiwan stage in the Japanese language during the colonial period, it was after Kuomintang moved to Taiwan in 1949 that Shakespeare studies began to flourish when scholars and theatrical experts from mainland China, such as Liang Shih-Chiu, Yu Er-Chang, Wang Sheng-shan and others brought Chinese Shakespeare to Taiwan. Since the 1980s, mainland Shakespeareans began to communicate actively with their colleagues in Taiwan. With the continuous efforts of Cao Yu, Fang Ping, Meng Xianqiang, Gu Zhengkun, Yang Lingui and many other scholars in mainland China and Chu Li-Min, Yen Yuan-shu, Perng Ching-Hsi and other scholars in Taiwan, communications and conversations on Shakespeare studies across the Taiwan Strait were gradually enhanced in recent years. Meanwhile, innovations in Chinese adaptations of Shakespeare have resulted in a new performing medium, Shake-xiqu, through which theatrical practitioners on both sides explore possibilities of a union of Shakespeare and traditional Chinese theatre. This paper studies some intricate relationship in the history of Shakespeare studies in mainland China and Taiwan from a developmental perspective and suggests opportunities for positive and effective co-operations and interactions in the future.

This paper seeks to interpret Good Words to Admonish the Age, the most important writing of the first Chinese Protestant pastor, Liang Fa (1789–1855), in its complex relations with the tradition of morality books (shan shu). By doing so, the paper attempts to show Liang's subversive adoption of an existing social and religious genre that enjoyed widespread acceptance at the time. While Liang affirms the significance of moral values, he also distinguishes those practices held by morality books as meritorious from actual moral uprightness. In contrast, moral good for Liang is a result of divine intervention (that is, salvation) and a Christian duty, thus transcending the conventional purpose of earthly reward or securing one's own fate for blessings. In crafting his Good Words, the morality-book tradition forms an essential point of contact that Liang appropriated and adapted for delivering his Christian message – a message that is also in competition with the conventional moral view of salvation. For Liang, these moral tenets, which he still holds dear after his conversion, now culminate in a theological knowledge of God and his salvation plan.

Abstract The Fanconi anemia (FA) pathway is a major player in the control of DNA replication integrity in response to replication stress. Germline defect in the pathway results in the FA syndrome characterized by developmental abnormalities, bone marrow (BM) failure, and genome instability which greatly elevates the incidence of cancers. A pivotal step in the activation of the FA DNA repair pathway is the monoubiquitination of the FANCD2 and FANCI proteins (ID2) by the FA core complex, a unique ubiquitin ligase complex which includes eight proteins (FANCA-FANCG, FANCL, and FAAP100) and UBE2T/FANCT. This monoubiquitination event enables the recruitment of the ID2 complex to chromatin and nuclear foci at sites of DNA damage. Cells with mutations in any of the FA core complex proteins lack the ability to monoubiquitinated ID2, making ID2 ubiquitination a convergence point in the pathway, with an estimation of>90% FA patients defective in this step. Additionally, somatic mutations In FA genes render tumor cells sensitive to DNA crosslinking agents, so identification of FA pathway defects provides an opportunity for therapeutic targeting. In search for additional potential target/substrate of this unique FA core ubiquitin ligase complex, we performed a high throughput genome-wide ubiquitin-specific proteomics (UbiScan) screen and found, in addition to the ID2 complex, many ubiquitinated proteins are dysregulated (mostly downregulated) in FA deficient cells compared with that of FA proficient cells. We used a Ubiquitin Remnant Motif (K- ∑-GG) Antibody Bead Conjugate (Cell Signaling Technology), a proprietary ubiquitin branch ("K- ∑-GG") antibody with specificity for a di-glycine tag that is the remnant of ubiquitin left on protein substrates after trypsin digestion, to enrich ubiquitinated peptides from trypsin digested cell samples (shNT vs shFANCA). This enrichment is followed by LC-MS/MS analysis for quantitative profiles of hundreds to over a thousand nonredundant ubiquitinated sequences. We were successful in demonstrating that under steady-state conditions (without proteasome inhibitor treatment), the ubiquitinated forms of both FANCD2 and FANCI proteins are much higher in control (shNT) HeLa cells compared with that of the cells depleted of FANCA (shFANCA). We then collaborated with the Cell signaling technology to perform a high throughput UbiScan® analysis of total ubiquitinated proteins both in total nuclei and chromatin fractions under replicative stress conditions. UbiScan® enables researchers to isolate, identify and quantitate large numbers of ubiquitin-modified cellular peptides with a high degree of specificity and sensitivity, providing a global overview of the ubiquitination sites in cellular proteins in cell and tissue samples without preconceived biases about where these modified sites occur. A total of 16,249 redundant modified peptide assignments to 7,856 modified sites for the Ubiquitin K-GG Remnant Motif Antibody were obtained. As expected, the amount of monoubiquitinated FANCD2 (at K651) and FANCI (at K523) were highly reduced in both the nuclear and chromatin fractions of Hela cells depleted of FANCA (shA). Consistent with the earlier findings, the amount of ubiquitinated ID2 proteins were extremely low in the chromatin fraction of the Hela cells depleted of FANCA. Since there are numerous ubiquitinated proteins found to be dysregulated in our UbiScan analyses, we used the following criteria to select the target proteins based on; a) -fold changes, and b) proteins that are known to participate in the DNA repair signaling pathways. We validated our UbiScan results by using an assay system to detect endogenous protein ubiquitination. We also found a significant reduction in the ubiquitination of several DNA repair-related proteins (found in our UbiScan analysis) in FANCA deficient cells. To assess FA pathway functions, we generated HAP1 and appropriate cells knock out of these select ubiquitinated target proteins by using CRISPR-Cas9 system. Then, the KO cells were examined for FA pathway functions. These results will be discussed. In conclusion, our findings reveal that the FA core ubiquitin ligase complex regulates (directly or indirectly) the ubiquitinated levels of many novel proteins outside of the ID2 complex, and these novel target proteins may provide important additional mechanistic insights into the FA DNA repair pathway. Disclosures No relevant conflicts of interest to declare.

Background: To explore the prescription rules of famous ancient physicians in the treatment of threatened miscarriage. Methods: Traditional Chinese Medicine (TCM) prescriptions for threatened miscarriage were screened out of Fu Ren Da Quan Liang Fang by Ziming Chen, Yi Zong Jin Jian by Qian Wu, and Fu Qing Zhu Nv Ke by Qingzhu Fu. Data were standardized and analyzed through the TCM inheritance auxiliary platform. Results: A total of 29 prescriptions for threatened miscarriage were screened. Dang Gui, E Jiao, Gan Cao, Chuan Xiong, Bai Shao were the top five frequently prescribed Chinese herbs. The common herb–herb combinations used by Ziming Chen contained E Jiao, Dang Gui, Chuan Xiong, Ai Ye, Cong Bai, and Sang Ji Sheng. Ren Shen, Gan Cao, and Bai Zhu were the common herbal groups used by Qingzhu Fu. Huang Qi, Shu Di Huang, Bai Shao, Dang Gui, and Gan Cao were one of Qian Wu’s core prescriptions, with Dang Gui and Chuan Xiong being the others. According to the analysis of four Qi, five flavors, and meridian tropism of the prescriptions, herbs with the warm nature, or with the sweet, pungent, bitter flavors topped the list of application. The top six meridian tropisms of high-frequency herbs were: liver, spleen, lung, kidney, heart, and stomach meridian. Conclusion: Based on the principle of restoring the balance within the organs and enriching Qi and blood, clinical treatment of threatened miscarriage involves invigorating the Chong and Ren channels, nourishing Yin, dispelling cold and wind, generating and activating blood, regulating and harmonizing Qi.

Abstract The efficacy of oral proprietary Chinese medicine in the prevention and treatment of primary osteoporosis was systematically evaluated through a network meta-analysis. The computer retrieved the four major Chinese databases of CNKI, VIP, Wang Fang, CBM in China and abroad, as well as the three English databases and Web of Science of Medline, EMbase and Cochrane Library. The search cycle was designed as the database was established until February 15, 2022, and screened randomized controlled trials of export taking proprietary Chinese medicine combined with conventional western medicine for the prevention and treatment of primary osteoporosis according to the system evaluation pre-formulated exclusion criteria. Literature screening and data extraction are completed by at least 2 clinical investigators alone. Bias assessment and mapping of included studies using the Revman software, using the Cochrane Bias Risk Assessment tool, using Stata 16. 0 Software for data acquisition and analysis. In the end, 19 RCTS were included, involving 8 kinds of oral proprietary Chinese medicines (You Gui Pills, Jin Tiange Capsules, Gu Shu Kang Granules, Qiang Bone Capsules, Gu Songbao Granules, Xianling Gu Bao Capsules, Hu Gu Capsules, Zuo Gui Pills). The results of the network meta-study are as follows: (1) in terms of improving clinical efficiency, conventional western medicine combined with Hu Gu Capsules has the best effect; (2) in terms of improving bone density, conventional western medicine combined with Xianling Gu Bao has the best effect. This network meta-analysis provides evidence for the efficacy of the above eight proprietary Chinese medicines in the treatment of patients with POP.

Exposure to insecticides containing organophosphate (OP) and neonicotinoid (NEO) compounds has been associated with adverse reproductive health outcomes. This study characterized and identified predictors of exposure to OP and NEO among 100 reproductive-age farmworkers from two intensive farming areas in Chiang Mai Province, Thailand, including 50 each from the Fang (FA) and Chom Thong (CT) districts. OP exposure was determined by measuring the urinary concentrations of six dialkylphosphates (DAPs), whereas NEO exposure was determined by measuring the urinary concentrations of NEO compounds and their metabolites (NEO/m). The most frequently detected OPs were diethylphosphate (DEP) and diethylthiophosphate (DETP), with DETP having the highest geometric mean (GM) concentration, 8.9 μg/g-creatinine. The most frequently detected NEO/m were N-desmethyl-acetamiprid (N-dm-ACE), imidacloprid (IMI), and thiamethoxam (THX), with IMI having the highest GM concentration, 8.7 μg/g-creatinine. Consumption of well water was the predominant determinant of OP and NEO exposure in this population. In addition to encouraging workers to use personal protective equipment, exposure of farmworkers to these compounds may be reduced by nation-wide monitoring agricultural insecticides and other pesticides in community drinking water resources.

Over the centuries, China still respectfully treasures rich Asian cultures, traditions, and customs. China is now famous all over the world for its mysterious wonders and cultural & natural heritages such as the Great Wall, the Forbidden City, the Terracotta Army, etc. The Chinese history is full with the exposition of outstanding features of Chinese culture such as great thoughts of Confucius, religious beliefs, traditional festivals and customs e.g., Chinese new year, language and calligraphy e.g., Shu Fa, four great inventions of ancient China e.g., papermaking, printing, gunpowder and the compass, traditional architecture and sculpture, traditional art forms, etc. The era of history of China before the time in power of Qin dynasty is known by name as the period of Warring States. This period started from 475 BC and ends at 221 BC. Seven Warring States were included in it i.e. Qin, Wei, Han, Yan, Chu, Zhao, and Qi. Zheng was the King of Qin, who started his journey to triumph over 6 states in the period of 230 BC. Qin was the 1st emperor of this unified state of China. Thus he was known by the name of “First Emperor of Qin” or “Qin Shi Huang”. This study provides a deep insight of Chinese history and it is illustrated that major achievements in Chinese culture and history are contributed in the era of Qin dynasty.

Tradition says that Yu, first ruler of the Xia Dynasty, was chosen by the “sage emperor” Shun as Shun's successor. The “Modern Text” Bamboo Annals (Jinben Zhushu jinian) dates this act of choice to the fourteenth year of Shun. (With E. L. Shaughnessy, “On the Authenticity of the Bamboo Annals,” Harvard Journal of Asiatic Studies 46 (1986), we accept this text as at least in part the text found in a royal tomb of Wei in A.D. 281.) Following D. Pankenier's argument (“Mozi and the Dates of Xia, Shang and Zhou,” Early China 9–10 [1983–85]), we date this event to 1953 B.C., the year of a dramatic five-planet conjunction. (K. Pang independently dated this conjunction to Yu's reign in his article “Extraordinary Floods in Early Chinese History and their Absolute Dates,” Journal of Hydrology 96 [1987].)We next use K. Pang's discovery (“Extraordinary Floods”) that there was an eclipse of the sun on 16 October 1876 B.C., that exactly satisfies descriptions in the Zuo zhuan (Zhao 17) and in the Bamboo Annals for Xia, Zhong Kang fifth year, of an eclipse associated with the (post-Han Shang shu) “Punitive Expedition of Yin” (except for the day-cycle in the Annals, which we assume to be a later calculation); i.e., it occurred on the first of the ninth lunar month (Xia calendar), the sun's location at the time (188å) was in lunar lodge Fang, and the eclipse was visible in the probable Xia capital area. No other eclipse within many centuries satisfies these criteria.Extending D. Nivison's theory (“The Dates of Western Chou,” Harvard Journal of Asiatic Studies 43 (1983)) that Western Zhou royal calendars began only after completion of mourning, i.e., two years after accession, we then assume that there were similar two-year mourning breaks between Xia royal calendars (possibly reflected in the irregular interregnums in the present Annals). For a demonstration of this chronology, see the chart on page 94.

The flow condensation heat transfer (FCHT) in plain channels has a variety of applications in many industrial sectors, and thus it is important to predict FCHT coefficients accurately. This paper compiled a large compound FCHT database containing 5607 data points and 30 fluids and presented a strategy for developing a new correlation of FCHT coefficients. The parameter range of the database is the hydraulic diameter D = 0.493–20 mm, vapor quality x = 0.003–0.992, mass flux G = 24–1533 kg/m2 s, heat flux q = 2.9–422 kW/m2, reduced pressure pr = 0.04–0.95, liquid Prandtl number Prl = 1.7–8.5, liquid Reynolds number Rel = 11.6–5.3 × 104, and gas Reynolds number Reg = 75.1–9.1 × 105. Based on the database and strategy, a new general correlation with substantially better accuracy was developed, which is applicable to plain channels of various sizes and a broad operational parameter range. It predicts the database with a mean absolute deviation (MAD) of 14.1%, while the best existing Δ T-independent correlation predicts the database with an MAD of 20.2%. The applicability of the new and 38 existing correlations to individual fluids was assessed. The new correlation performs best for 8 of the 14 fluids that have more than 50 data points in the entire database, while the most accurate existing one performs best only for 2 of them. The Fang number Fa plays an important role in the new correlation, implying that it relates to the fundamental mechanisms of both boiling and condensation heat transfer.

Als tallers infantils d’art de l’Escola Municipal d’Art Illa de Sabadell procurem fer treballs significatius que tenen a veure amb les biografies dels nens i les seves famílies. En aquest, hem fet uns plats de ceràmica pintats amb el relat del dia que els pares es van veure per primer cop.Des de l’antiguitat, la ceràmica sempre ha estat un suport per facilitar la vida i alhora ha estat portadora d’elements simbòlics que acompanyen l’esperit de la seva funcionalitat, amb decoracions que donen força i màgia per al viure dels usuaris.Fer un plat amb fang, commemoratiu, és un acte simbòlic: un plat en què ens menjarem possiblement el nostre futur. Com expliquen Jodorowsky (Jodorowsky i Costa, 2011) i sobretot Stephan Hausner (2013) quan parlen del contracte inicial que fa una parella quan es troba, moltes clàusules complexes dels pares les paguen els fills, que intenten compensar i solucionar coses i ho acaben pagant amb la seva salut.Aquests tipus de propostes artisticoeducatives desperten relats dintre de les famílies del nostre alumnat, i també professorat, i es produeix a l’escola un fet que sempre ens encomana incloure-hi Carles Parellada des de la pedagogia sistèmica: la reconciliació biogràfica, la creació d’un relat del vincle que ens ajuda a donar sentit i força per viure el present.Aquesta proposta es va fer amb l’alumnat del màster en Pedagogia Sistèmica a l’Institut Gestalt i la Fundació URV, i ens va permetre descobrir en la pròpia pell la importància d’aquest relat fundacional de la parella que ens va fer. Sigui el que sigui el que va passar entre els pares, si es tracta del nostre origen és prou important saber el que va passar per fer el nostre propi relat present del viure. Es van incorporar moviments sistèmics a través dels quals es van fer emocionants descobriments.

The Internet of Vehicles (IoV) is witnessed to play the leading role in the future of Intelligent Transportation Systems (ITS). Though many works have focused on IoV improvement, there is still a lack of performance due to insufficient spectrum availability, lower data rates, and the involvement of attackers. This paper considers all three issues by developing a novel mmWave-assisted Cognitive Radio based IoV (CR-IoV) model. The integration of CR in IoV resolves the issue of spectrum management, while mmWave technology ensures symmetry in acquiring higher data rates for Secondary Users (SUs). With the proposed mmWave-assisted CR-IoV model, symmetric improvements in network performance were achieved in three main areas: security, beamforming, and routing. Optimum detection mechanisms isolate malicious Secondary Users (SUs) in the overall network. First, Spectrum Sensing Data Falsification (SSDF) attack is detected by a Hybrid Kernel-based Support Vector Machine (HK-SVM), which is the lightweight Machine Learning (ML) technique. Then, the Primary User Emulation (PUE) attack is detected by a hybrid approach, namely the Fang Algorithm-based Time Difference of Arrival (FA-TDoA) method. Further, security is assured by validating the legitimacy of each SU through a Lightweight ID-based Certificate Validation mechanism. To accomplish this, we employed the Four Q-curve asymmetric cryptographic algorithm. Overall, the proposed dual-step security provisioning approach assures that the network is free from attackers. Next, beamforming is performed for legitimate SUs by a 3D-Beamforming algorithm that relies on Array Factor (AF) and Beampattern Function. Finally, routing is enabled by formulating Forwarding Zone (FZ) based on the forwarding angle. In the forwarding zone, optimal forwarders are selected by the Multi-Objective Whale Optimization (MOWO) algorithm. Here, a new potential score is formulated for fitness evaluation. Finally, the proposed mmWave-assisted CR-IoV model is validated through extensive simulations in the ns-3.26 simulation tool. The evaluation shows better performance in terms of throughput, packet delivery ratio, delay, bit error rate, and detection accuracy.

Abstract Background: Pediatric sarcomas represent approximately 12% of all pediatric cancers with over 100 subtypes. Although some molecular detection techniques can be used to assist the diagnosis and treatment of pediatric sarcoma, it still remains challenging due to overlapping morphological features and limited biopsy specimens. Here we performed next generation sequencing (NGS) to analyze the molecular profiles of pediatric and adult sarcomas in China. Methods: A total of 700 Chinese patients with sarcoma were collected. The tumor tissue and matching blood specimens were tested by the integrative DNA and RNA sequencing panel Onco Panscan plus࣪ at Genetronhealth. Results: Of the 700 sarcoma patients, there were 224 children (32%) and 476 adults (68%). Difference from the almost same ratio of men to women in adults, there were more males (n = 134) than females (n = 90) in children. Rhabdomyosarcoma was the most common soft tissue tumor in children, accounting for 24.1% (n = 54), followed by Ewing's sarcoma (9.8%, n = 22) and Osteosarcoma (8.5%, n = 34), while Angiosarcoma (3.8%, n = 18) and Fibrosarcoma (3.6%, n = 17) were the most common in adults. Based on mutation types, the frequent alterations were missense mutations (n = 326, 51.2%), fusions (n = 129, 20.3%) and copy number variants (n = 66, 10.4%) in children with a mean of 3 mutations per patient, and missense mutations (n = 1438, 66.8%), fusions (n = 221, 10.3%) and truncating mutations (n = 215, 10.0%) in adults with an average 5 mutations. TP53 (12.9%, n = 29), EWSR1 (8.9%, n = 20) and ALK mutations (6.7%, n = 15) were common in children, which was distinct from TP53 (29.4%, n = 140), NF1 (4.8%, n = 23), CTNNB1 (4.2%, n = 20) and RB1 (4.2%, n = 20) in adults. Based on NGS results, pathological subtypes could be confirmed in 40.2% (90/224) and 48.1% (229/476) of children and of adults sarcoma patients, respectively. In addition, we identified a total of 72 drug-targeted gene mutations in the 57 children patients, of which 35 (48.6%) gene mutations could be targeted by FDA-approved drugs. In adults, 256 drug-targeted gene mutations were detected and the proportion of actionable mutations was up to 78.9% (n = 202). Conclusion: The genomic landscape of pediatric sarcomas is different from that of adults. NGS aids in the subtype classification and clinical guidance of pediatric sarcomas, providing evidence for personalized treatments with clinical benefit. Citation Format: Weisong Cai, Fang Liu, Xuejiao Liu, Xiaojuan Wang, Chunyang Wang, Tonghui Ma, Shu Li. Next-generation sequencing (NGS) reveals different molecular profiles of pediatric sarcoma in children and adults [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5756.

Abstract Background Colorectal cancer (CRC) has a high incidence and mortality. In CRC, the lung is the most common extra-abdominal site of metastasis. Understanding the genetic characteristics and clonal evolution of primary tumor and lung metastases (LM) will help oncologists better outline treatments. To date, few studies for CRC with LM exist. As such, revealing the genetic characteristics and clonal evolution of resectable CRC with LM was the goal of this study. Methods From January 2012 to July 2019, clinical data and formalin-fixed, paraffin-embedded samples were collected from 33 Chinese CRC patients. Nineteen (19) primary tumors and 10 matched LM samples were examined using a 450 cancer gene next-generation sequencing assay. Genomic alterations, including single base substitutions, indels, copy number variations (CNVs), and gene fusion and rearrangement, were assessed. A phylogenetic reconstruction of sequencing data using LICHeE was also performed for each patient. Results Median patient age was 60 years old (48-67 years), with most having right lung metastasis. A significant difference existed between single (21/33, 63.6%) and multiple LM (12/33, 36.4%) (P < 0.01). Median disease-free survival was 20 months (95% CI, 0-84), from primary CRC to LM. The most frequently mutated genes were TP53 (90%), APC (90%), KRAS (53%), MUC16 (26%), ARID1A (21%). A comparison of mutational profiles for primary and matched LM samples revealed that 65.70% of alterations were consistent and that the most frequently mutated genes, including APC, TP53, and KRAS, of the primary tumor were completely consistent with LM. However, some differences between metastatic and primary samples were determined. More CNVs were found in primary samples (11.4% vs. 4.6%), indicating that CNVs are early molecular events for tumorigenesis and disease progression. SMAD4 was only present in two primary samples, while LRP1B was only present in two LM samples. Clonal evolution for eight patients indicated that phylogenetic structure was similar across patients. All patients had at least seven mutations on trunks. In trunks, TP53 (87.5%), APC (87.5%), KRAS (50%), and LRP2 (37.5%) were frequently identified. A new actionable gene (BRCA2) emerged in LM sample was identified in one patient. The mean value of TMB for the primary was almost the same as that for the LM site (5.3 vs. 5 muts/Mb, P > 0.05). Conclusions Our data indicated that the genetic characteristics and clonal evolution of LM are highly consistent with those of the primary tumor, suggesting that suitable treatments can be selected based on the genetic characteristics of primary tumor in CRC patients with LM that cannot be surgically treated. Citation Format: Yong Cheng, Ming-Jian Ge, Peng Dong, Yan-Yu Qiu, Xin-Peng Shu, Jin-Dou Li, Jun-Wei Wang, Xiao-Fang Qi. Genetic characteristics & clonal evolution of Chinese resectable colorectal cancer patients with lung metastases [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5133.

Abstract Abstract 1798 Rituximab maintenance for the treatment of patients with follicular lymphoma: systematic review and meta-analysis of randomized trials - 2010 update. Liat Vidal, Anat Gafter-Gvili, Gilles Salles, Martin Dreyling, Michele Ghielmini, Shu-Fang Hsu Schmitz, Ruth Pettengell, Mathias Witsenz-Harig, Ofer Shpilberg. Follicular lymphoma (FL) is characterized by slow growth and an initially high rate of response to treatment, but patients typically relapse and experience progressive disease. Rituximab in combination with chemotherapy has been shown to improve overall survival (OS) in patients with FL compared with chemotherapy alone. In order to evaluate the effect of maintenance treatment with rituximab on the OS of patients with FL we performed a systematic review and meta-analysis in 2007. Updated results from these studies and new clinical trials are reported here. Methods: A systematic review and meta-analysis of randomized controlled trials that compared rituximab maintenance therapy with observation or treatment at relapse (no maintenance therapy). In June 2010 we updated our 2007 search in The Cochrane Library, MEDLINE, LILACS, conference proceedings, and databases of ongoing trials. Two reviewers independently assessed the quality of the trials and extracted data. HRs for time-to-event data were estimated and pooled. Results: The search in 2007 identified five eligible trials including 1143 adult patients. The results of the meta-analysis of 985 patients with FL were previously reported. The present search identified six additional included trials; three of them had no current available outcome data. Three of the trials that were included in our first report had long term outcomes that were available for the current meta-analysis. Patients treated with maintenance rituximab had statistically significant better OS compared to patients in the observation arm or patients treated at relapse (HR for death 0.75, 95% confidence interval (CI) 0.61 to 0.91, 2283 patients, Figure). Patients with refractory or relapsed (i.e., previously treated) FL had a significant survival benefit with maintenance rituximab therapy (HR for death 0.72, 95% CI 0.57 to 0.91, 909 patients), whereas previously untreated patients did not (HR for death 0.83, 95% CI 0.56 to 1.23, 1374 patients). Progression free survival was improved in each of the included trials, pooled HR 0.54 95% CI 0.48 to 0.61, n=2283. This effect was consistent both in patients who received rituximab maintenance after their first induction therapy (HR 0.53, 95% CI 0.44 to 0.63, n=1374), and in those who received maintenance rituximab after two or more inductions (HR 0.63, 95% CI 0.50 to 0.79, n=804), and following different induction therapies: rituximab alone (HR 0.54, 95% CI 0.40 to 0.73, n=240), chemotherapy alone (HR 0.49, 95% CI 0.37 to 0.66, n=308), and rituximab-chemotherapy (HR 0.58, 95% CI 0.48 to 0.70, n=1352). The rate of infection-related adverse events was higher with rituximab maintenance treatment (Risk ratio (RR) 1.99, 95% CI 1.21 to 3.27). The rate of grade 3/4 adverse events was higher with rituximab maintenance (RR 1.47 95% CI 1.19 to 1.83). Conclusions: Rituximab maintenance improves OS and disease control in patients with FL after a successful induction therapy. The accumulating data from new and updated clinical trials did not change the results of our former meta-analysis. While a clear survival benefit is shown only for patients with relapsed or refractory FL, progression free survival is improved after first induction as well as after two or more inductions. Disclosures: Salles: Roche: Honoraria. Dreyling:Roche: . Ghielmini:Roche: Consultancy. Pettengell:Roche: Consultancy, Honoraria. Shpilberg:Roche: Consultancy.

Abstract Background: METex14 mutations was reported in 3~4% of NSCLC patients and became a new target in the treatment of NSCLC. SCC244 is a highly selective and potent oral MET inhibitor. This is the first report of data from an ongoing single-arm phase II study of SCC244 in NSCLC patients with METex14 mutations (GLORY study). Methods: GLORY study is an open label, international, multi-center, single-arm phase II study to evaluate the efficacy and safety of SCC244 in patients with locally advanced or metastatic NSCLC harboring METex14 mutations which was confirmed by central laboratory. The enrolled patients have either failed one or two prior lines of systemic therapies or been not eligible/refused chemotherapy after being well-informed. SCC244 was taken orally at a dose of 300 mg once daily in 21-day treatment cycles until disease progression or intolerable toxicity. Tumor was evaluated every 6 weeks for the first 8 treatment cycles and every 9 weeks thereafter. The primary endpoint was objective response rate (ORR) assessed by blinded independent review committee (BIRC) per RECIST 1.1, secondary endpoints include ORR by investigator assessment (INV), duration of response (DoR), time to response (TTR) and safety etc. Post-hoc analysis was done to explore the intracranial anti-tumor activity. Results: At data cut-off on May 6th, 2021, a total 73 patients screened from 163 patients in 42 sites were treated at 300 mg QD dose and had ≥2 post-baseline tumor assessments or discontinued for any reason. 69 of them were with METex14 mutation confirmed by central laboratory. In the 69 patients, ORR by BIRC was 60.9% (95% CI: 48.4%, 72.4%) overall, 66.7% (95% CI: 50.5, 80.4) and 51.9% (95% CI: 31.9, 71.3) in treatment naïve and previously treated patients respectively. Median DoR was 8.2 months (95% CI: 4.8, NE) and median PFS was 7.6 months (95% CI: 4.2, NE), tumor response from 30 of 42 responders was still ongoing. The response occurred fast with a median TTR of 1.4 months (range: 1.2, 4.2). Partial response was observed in 8 of 10 patients with brain metastasis. 5 patients who had brain metastasis selected as targeted lesion had intracranial response by INV with a median intracranial tumor shrinkage of 57% (range: 34%, 71%). The most common (≥20%) treatment-related adverse events (TRAEs) of any grade were peripheral edema, headache, nausea, loss of appetite, hypoalbuminemia, ALT increase and vomiting. The incidence of ≥ grade 3 TRAEs was 43.8%. TRAEs leading to treatment discontinuation occurred in 6.8% patients, among which peripheral edema was the most common (4.1%). Conclusions: The data shows high and robust efficacy of SCC244 in NSCLC patients with METex14 mutations across treatment lines and encouraging intracranial anti-tumor activity. The safety profile was favorable with manageable toxicity. The data supports SCC244 as a valuable targeted treatment option for METex14 NSCLC patients. Citation Format: Shun Lu, Yongfeng Yu, Jianya Zhou, Koichi Goto, Xingya Li, Jun Sakakibara-Konishi, Kazumi Nishino, Tanaka Kentaro, Lin Wu, Xuhong Min, Wei Zhang, Dingzhi Huang, Yongqian Shu, Chengzhi Zhou, Min Li, Xiaorong Dong, Chong Bai, Lu Li, Jiuwei Cui, Li Zhang, Lejie Cao, Xiaoling Li, AiMin Zang, Haruki Kobayashi, Yiping Zhang, Yan Yu, Xiuwen Wang, Terufumi Kato, Shoichiro Yamamoto, Yuki Shinno, Xiaoyan Lin, Yanqiu Zhao, Yanping Hu, Qitao Yu, Ziping Wang, Masahiro Kodani, Jian Fang, Jialei Wang, Meiqi Shi, Diansheng Zhong, Wen Dong, Hiroshi Tanaka, Yasuto Yoneshima, Minghui Sun, Jun Zhou, Qiuxia Wu, Meng Li. Phase II study of SCC244 in NSCLC patients harboring MET exon 14 skipping (METex14) mutations (GLORY study) [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr CT034.

Abstract Background: Chemotherapy is the main treatment for metastatic triple-negative breast cancer (mTNBC). However, mTNBC patients (pts) is often associated with chemotherapy resistance and poor prognosis. Biomarkers that can effectively predict the efficacy of chemotherapy for mTNBC are currently lacking. Detection of circulating tumor DNA (ctDNA) in plasma by liquid biopsy is a minimally invasive and highly sensitive method, which has been wildly used in the clinic and plays an important role in tumor diagnosis, efficacy evaluation, residual and recurrent tumor detection, etc. But yet, the clinical application of ctDNA in mTNBC remains relatively scarce. Here, for the first time, we set to explore the possibility of ctDNA as a biomarker for predicting chemotherapy response and prognosis in mTNBC.Methods: From May 2018 to October 2020, 38 mTNBC pts who received less than 3rd line standard chemotherapy were prospectively included. Tumor tissues were obtained prior chemotherapy, and plasma for ctDNA were collected at baseline, a day before the 3rd cycle chemotherapy and at disease progression. Next-generation sequencing (NGS,457 genes panel) was performed on all samples for mutation detection. And the ctDNA fraction, maximum variant allele frequency (max-VAF), tumor mutation burden (TMB) and other variate were calculated, further combined with clinical data, chemotherapy response and survival of pts for statistical analysis.Results: Finally, 109 blood samples and 13 tissue samples were detected by NGS. A total of 214 mutation genes and 397 mutation sites were detected. The mutation types included missense mutation, nonsense mutation, non-/frameshift insertion, non-/frameshift deletion, non-/frameshift substitution, splicing mutation and so on. The genes with the top 5 mutation frequencies were TP53 (32/38, 84.21%), PIK3CA (14/38, 36.84%), KMT2C (8/38, 21.05%), PTEN (6/38, 15.79%), NOTCH4 (6/38, 15.79%), respectively. The same variants were identified in 12 of 13 pts in paired plasma and tissue, with a concordance rate of 92.3%. The mutation rate in plasma ctDNA was significantly lower than that in tissues (15.26% ± 12.52% vs. 28.88 ± 16.54%, P < 0.001) but was still positively correlated with that in tissues (r = 0.306, P = 0.049). The median progression-free survival (mPFS) of pts with GNAS mutation was shorter than GNAS wild-type pts (3.000 vs. 6.100 months, P = 0.014). The area under curve of ctDNA fraction (0.812, P = 0.043), max-VAF (0.817, P = 0.043) and TMB (plasma) (0.759, P = 0.053) in predicting chemotherapy resistance or disease progression were larger than CEA (0.491, P = 0.888), CA125 (0.574, P = 0.243) and CA153 (0.482, P = 0.778). CtDNA fraction (r = 0.482, P < 0.001), max-VAF (r = 0.489, P < 0.001), TMB (plasma). (r = 0.419, P < 0.001) were correlated with chemotherapy response measured by RECIST v1.1 in CT imaging, while CEA (r = -0.024, P = 0.808), CA125 (r = 0.111, P = 0.266) and CA153 (r = -0.017, P = 0.865) had no correlation with chemotherapy response. The mPFS of pts with ctDNA fraction ≤ 50% or max-VAF ≤ 0.4 at baseline was significantly longer than that of pts with ctDNA fraction > 50% (6.100 vs. 3.430 months, P = 0.006) or max-VAF > 0.4 (6.100 vs. 3.430 months, P = 0.047). The elimination of mutations or the decrease of mutation rate in plasma ctDNA after 2 cycles of chemotherapy showed better chemotherapy response, while recurrence of mutations, increase of mutation rate and emergence of new mutations showed chemotherapy resistance.Conclusions: Mutations in tumor tissues and plasma ctDNA of mTNBC pts detected by NGS have high consistency. And compared with CT imaging and traditional tumor markers, dynamic monitoring ctDNA can more aptly reflect the change of whole-body tumor burden, better predict the chemotherapy response and prognosis in mTNBC. Citation Format: Huihui Li, Yajing Chi, Sha Yin, Bo Yu, Mu Su, Baoxuan Zhang, Ling Qiang, Guohua Ren, Lihua Song, Bing Bu, Shu Fang, Mao Shang, Qiaorui Tan, Xiaochu Man. Dynamic monitoring of circulating tumor DNA can predict chemotherapy response and prognosis in metastatic triple-negative breast cancer patients [abstract]. In: Proceedings of the 2021 San Antonio Breast Cancer Symposium; 2021 Dec 7-10; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2022;82(4 Suppl):Abstract nr P1-08-27.

Abstract Artificial photosynthesis of solar fuels is deemed as one of the Holy grails of renewable energy technology for simultaneously solving energy and environmental issues. At the present, it is one of the most involved programs in the international Mission Innovation Challenge for Accelerating the Clean Energy Revolution. Solar-driven water splitting and CO2 conversion are the main research application of artificial photosynthesis. However, these reactions are extremely challenging due to energetically uphill (G>0) and non-spontaneous multi-electron transfer processes, which are difficult to be understood by traditional knowledge of catalysis. An efficient solar energy conversion system must simultaneously deal well with light absorption, charge separation and transfer, surface redox reactions. Particularly, efficient charge separation and transfer by retarding back electron transfer, are often regarded as the key determining steps for overall solar energy conversion. To solve the high recombination rates of photogenerated electron-hole pairs and their low reduction and oxidation abilities in a single photocatalyst, heterojunction manipulation is urgently required. Two mainstream heterojunctions—type-II and Z-scheme heterojunctions have been widely acknowledged [1]. Recently, lead halide perovskites (LHPs) with the chemical formula of ABX3, where A is an organic or inorganic cation (A= Cs+, MA+, FA+), B is Pb2+, and X is a halogen anion (Br-, Cl-, I-), such as CH3NH3PbI3,FAPbI3, and CsPbBr3 have been widely investigated as auspicious semiconductor photocatalysts for photocatalytic H2 production and photocatalytic CO2 reduction owing to their impressive photoelectrochemical properties, facile to synthesize, high carrier mobility, low exciton binding energy, and long carrier lifetime [2]. However, the high toxicity and notorious instability upon exposure to light, moisture, and high temperature are the major obstacles to their practical use.Therefore, developing alternative lead-free semiconductor photocatalysts with similar optoelectronic properties to the LHPs is highly needed. Inorganic halide double-perovskites and analogous oxide double-perovskites with the chemical formula of A2B'B"X6 and A2B'B"O6, respectively, are layered 3D materials, which have been considered as a novel ecofriendly visible light responsive semiconductor photocatalysts to replace the toxic lead-halide perovskite. The main feature of the halide and oxide double-perovskites is that their structures can be accommodated with different transition metal combinations on B' and B" site cations to tune their intrinsic properties such as light absorption, carrier mobilities, chemical diversity, and so on. Theoretically, an auspicious photocatalytic activity can be realized from them owing to their impressive photophysical properties. However, poor charge separation and severe charge recombination have restricted their practical photocatalytic application. Recently, several halides and oxides double perovskites have been demonstrated as visible light-responsive photocatalysts for photocatalytic CO2 reduction and photocatalytic half-reaction (oxygen and hydrogen evolution reactions) such as Cs2AgBrBr6, Cs2AgSbBr6, Sr2CoTaO6, Sr2CoWO6, etc. [3-5]. However, for oxide double perovskites even though they have shown bifunctional photocatalytic oxygen and hydrogen two half-reactions with visible light but their potential as photocatalytic CO2 reduction and one-step overall water splitting have not been achieved so far. Therefore, further improvement of the material design and synthesis by assembling heterostructure based on two eco-friendly halide and oxide double perovskites may play a key role in achieving high efficient photocatalytic performance under visible-light-irradiation. Thought is a challenging task, but holds great potential in advancing science and technology in photocatalysis. References Liao, C. Li, S.-Y. Liu, B. Fang, H. Yang, Emerging frontiers of Z-scheme photocatalytic systems, Trends in Chemistry. 4 (2022) 111–127. -C. Wang, N. Li, A.M. Idris, J. Wang, X. Du, Z. Pan, Z. Li, Surface Defect Engineering of CsPbBr3 Nanocrystals for High Efficient Photocatalytic CO2 Reduction, Solar RRL. 5 (2021) 2100154. Idris, A. M.; Liu, T.; Shah, J. H.; Zhang, X.; Ma, C.; Malik, A. S.; Jin, A. Solar RRL 2020, 4 (3), 1900456. Idris, A. M.; Liu, T.; Hussain Shah, J.; Han, H.; Li, C. ACS Sustainable Chemistry&Engineering 2020, 8 (37), 14190-14197. Wang, H. Huang, Z. Zhang, C. Wang, Y. Yang, Q. Li, D. Xu, Lead-free perovskite Cs2AgBiBr6@g-C3N4 Z-scheme system for improving CH4 production in photocatalytic CO2 reduction, Applied Catalysis B: Environmental. 282 (2021).

Imaging buried geological boundaries is one of a major objective during the interpretation of magnetic field data in Geophysics. Therefore, edge detection and edge enhancement techniques assist a crucial role on this aim. Most of the existing edge detector methods require to obtain special points such as in general the maxima of the resulting image. One of the useful tools in estimating edges from magnetic data is the tilt angle of the analytical signal amplitude due to its value slightly dependence on the direction of magnetization. In this study, the maxima of the tilt angle of analytical signal amplitudes of the magnetic data was determined by a curvature-based method. The technique is based on fitting a quadratic surface over a 3×3 windows of the grid for locating any appropriate critical point that is near the centre of the window. The algorithm is built in Matlab environment. The feasibility of the algorithm is demonstrated in two cases of synthetic data as well as on real magnetic data from Tu Chinh-Vung May area. The source code is available from the authors on request.ReferencesAkpınar Z., Gürsoy H., Tatar O., Büyüksaraç A., Koçbulut F., Piper, JDA., 2016. Geophysical analysis of fault geometry and volcanic activity in the Erzincan Basin, Central Turkey, Complex evolution of a mature pull-apart basin. Journal of Asian Earth Sciences, 116, 97-114. Beiki M., 2010. Analytic signals of gravity gradient tensor and their application to estimate source location, Geophysics, 75(6), 159-174.Blakely R. J., and Simpson R.W., 1986. Approximating edges of source bodies from magnetic or gravity anomalies, Geophysics, 51, 1494-1498.Chen An-Guo, Zhou Tao-Fa, Liu Dong-Jia, Zhang Shu, 2017. Application of an enhanced theta-based filter for potential field edge detection: a case study of the LUZONG ORE DISTRICT, Chinese Journal of Geophysics, 60(2), 203-218.Cooper G.RJ., 2014. Reducing the dependence of the analytic signal amplitude of aeromagnetic data on the source vector direction, Geophysics, 79, 55-60.Cordell L., 1979. Gravimetric Expression of Graben Faulting in Santa Fe Country and theEspanola Basin, New Mexico. In Ingersoll, R.V., Ed., Guidebook to Santa Fe Country, New Mexico Geological Society, Socorro, 59-64.Cordell L and Grauch V.J.S., 1985. Mapping Basement Magnetization Zones from Aeromagnetic Data in the San Juan Basin, New Mexico, The Utility of Regional Gravity and Magnetic Anomaly Maps, Society of Exploration Geophysicists, Tulsa, 181-197.Hsu S.K., Coppense D., Shyu C.T., 1996. High- resolution detection of geologic boundaries from potential field anomalies: An enhanced analytic signal technique, Geophysics, 61, 1947-1957.Le D.C., Application of seismic exploration methods to identify geological structural characteristics supporting for hydrocarbon potential assessment in TuChinh - Vung May basin, Ph.D. Thesis, Hanoi University of Mining and Geology.Li X., 2006. Understanding 3D analytic signal amplitude: Geophysics, 71(2), 13-16.Miller H.G. and Singh V., 1994. Potential Field Tilt a New Concept for Location of Potential Field Sources, Journal of Applied Geophysics, 32, 213-217.Nabighian M.N., 1972. The analytic signal of two-dimensional magnetic bodies with polygonal cross-section: Its properties and use of automated anomaly interpretation, Geophysics, 37, 507-517.Nguyen N.T., Bui V.N., Nguyen T.T.H., 2014. Determining the depth to the magnetic basement and fault systems in Tu Chinh - Vung May area by magnetic data interpretation, Journal of Marine Science and Technology, 14(4a), 16-25.Nguyen X.H, San T.N, Bae W., Hoang M.C, 2014. Formation mechanism and petroleum system of tertiary sedimentary basins, offshore Vietnam, Energy Sources, Part A, 36, 1634-1649.Phillips J.D., Hansen R.O. and Blakely R.J., 2007. The use of curvature in potential-field interpretation, Exploration Geophysics, 38(2), 111-119.Rao D.B., and Babu N.R., 1991. A rapid method for three-dimensional modeling of magnetic anomalies, Geophysics, 56(11), 1729-1737.Roest W.R., Verhoef J., and Pilkington M., 1992. Magnetic interpretation using the 3-D analytic signal, Geophysics, 57, 116-125.Tran N., 2017. Sediment geology of Vietnam, VNU Press.Tran T.D., Tran N., Nguyen T.H., Dinh X.T., Pham B.N., Nguyen T.T., Tran T.T.T.N., Nguyen T.H.T., 2018. The Miocenedepositional geological evolution of Phu Khanh, Nam Con Son and Tu Chinh - Vung May basins in Vietnam continental shelf, VNU Journal of Science: Earth and Environmental Sciences, 34(1), 112-135.Vo T.S., Le H.M., Luu V.H., 2005. Three-dimensional analytic signal method and its application in interpretation of aeromagnetic anomaly maps in the Tuan Giao region, Proceedings of the 4th geophysical scientific and technical conference of Vietnam, Publisher of Science and Engineering 2005.Wijns C, Perez C and Kowalczyk P, 2005, Theta map: Edge detection in magnetic data, Geophysics, 70, 39-43.

In this study, a look on Fang number (Fa) is presented. The Fa was introduced recently in 2013 by Professor Xiande Fang to provide great facilitation in describing flow boiling heat transfer coefficients. It is defined as the product of two terms. The first term is the ratio of buoyancy force to gravitational force, which has effects on bubble departure. The second term is the ratio of surface tension force to inertial force, which affects bubble formation. As a result, Fa is associated with the formation and departure of bubbles. The Fa will be expressed by using a combination of the Eötvös number (Eo), Froude number (Fr), and Weber number (We). Based on this study, it is clear that existing dimensionless numbers in literature, i.e., Eötvös number, Froude number, Weber number, and their combinations can be used to describe flow boiling heat transfer coefficients. This combination of existing non-dimensional groups (Eo, Fr, and We) leads to good correlation with flow boiling data of different working fluids such as CO2, R134a, and R22.

Liu Fang-Ju 劉芳如 & Cheng Shu-Fang 鄭淑方 2015. Guoli GugongBowuyuan cang Menggu wenwu huibian 國立故宮博物院藏蒙古文物彙編 – Cultural Relics of the Mongols in the National PalaceMuseum Collection – Тайваний Үндэсний "Хааны ордон" музей дэхМонголын түүх, соёлын өв дурсгалууд. Taibei: National PalaceMuseum. ISBN 9789575627348.Hu Jinshan 胡進杉 et al. (eds.) 2015. An-mani-bami-hou. Yuan cang Zangchuan fojiao wenwu tezhan. 唵嘛呢叭咪吽. 院藏藏傳佛教文物特展– Oṃ-maṇi-padme-hūṃ. Tibetan Buddhist Art in the National PalaceMuseum. Taibei: National Palace Museum. ISBN 9789575627652.

Fanconi anemia (FA) genes play critical roles in the repair of DNA lesions. Non-FA (or underlying FA) patients harboring heterozygous germline FA gene mutations may also face an increased risk of developing bone marrow failure, primary immunodeficiency disease, and hereditary cancer predisposition syndromes. We report a female patient who suffered from ovarian cancer at 50 years of age. During the initial treatment, six cycles of docetaxel and carboplatin (DC) combination chemotherapy were administered followed by two cycles of docetaxel maintenance therapy. Then, she received a routine follow-up every 3 months for the next 3 years, and all the results of the examination and laboratory tests were normal. Unfortunately, at 54 years of age, she developed a secondary cancer of therapy-related (t-) chronic myelomonocytic leukemia (t-CMML). After two courses of a highly intensive induction chemotherapy regimen with DAC (decitabine) and HAA (homoharringtonine, cytarabine), the patient suffered from severe and persistent bone marrow failure (BMF). Targeted next-generation sequencing (NGS) of a panel of 80 genes was performed on her initial bone marrow aspirate sample and identified PTPN11, NRAS, and DNMT3A somatic mutations. In addition, RNA sequencing (RNA-seq) revealed a rare NUP98-HOXC11 fusion. Whole-exome sequencing (WES) verified RAD51C, BRIP1, PALB2, and FANCG heterozygous germline mutations of the FA pathway, which were further confirmed in buccal swab samples by Sanger sequencing. For this patient, we hypothesized that an altered FA pathway resulted in genomic instability, hypersensitivity to DNA-crosslinking agents or cytotoxic chemotherapeutics, and unsuccessful DNA damage repair. Consequently, she developed ovarian cancer and secondary t-CMML and then suffered from BMF and delayed post−chemotherapy bone marrow recovery after several chemotherapy courses. This case highlights the importance of genetic counseling in patients with hematopoietic neoplasms with high clinical suspicion for carrying cancer susceptibility gene mutations, which require timely diagnosis and personalized management.

Aims: The study aimed to conduct a meta-analysis to determine the abnormalities of white matter in patients with type 2 diabetes mellitus (T2DM) by identifying the consistency of diffusion tensor imaging (DTI).Method: The literature for DTI comparing patients with T2DM with controls published before October 30, 2020, were reviewed in PubMed, Web of Science, Embase, CNKI, and Wan Fang databases. The meta-analysis was performed using the activation likelihood estimation (ALE) method, including 12 reports and 381 patients with T2DM.Results: The meta-analysis identified 10 white matter regions that showed a consistent reduction of fractional anisotropy (FA) in patients with T2DM, including genu of the corpus callosum, the body of corpus callosum, bilateral anterior corona radiata, bilateral superior corona radiata, bilateral cingulum, and bilateral superior fronto-occipital fasciculus.Conclusion: This study revealed the abnormal characteristics of white matter in T2DM, which would be helpful to understand the underlying neuropathological and physiological mechanisms of T2DM and provide evidence for clinical diagnosis and treatment.

Introdução: As análises clínicas estão dentro das competências que podem ser desenvolvidas pelo profissional farmacêutico, em que este, por sua vez, necessita de conhecimentos teórico-práticos em diversas disciplinas, fazendo com que seja imprescindível o desenvolvimento de estágios para se poder atuar nesta área. Objetivo: Levando em consideração a importância que o estágio em análises clínicas e suas práticas apresentam para o futuro profissional farmacêutico, o presente trabalho teve por objetivo descrever as atividades realizadas durante o estágio obrigatório em análises clínicas. Materiais e métodos: Tratou-se de um estudo descritivo, do tipo relato de experiência, realizado entre junho a agosto de 2018, no Laboratório de Análises Clínicas do Hospital Universitário Alcides Carneiro. Resultados: No setor de urinálise, o estagiário deveria desenvolver junto ao farmacêutico a análise física, química e microscópica da urina, enquanto que no setor de bioquímica todas as análises eram semiautomatizadas, em que havia a análise de sangue, soro, plasma, líquor e urina. Em microbiologia o estagiário observava a coleta de diferentes materiais biológicos, semeava as amostras, fazia inóculos e utilizava-se de máquinas que permitiam a incubação, identificação e o perfil de sensibilidade dos microrganismos isolados. No setor de hematologia houve o desenvolvimento de atividades voltadas para microscopia, coloração de lâminas e análises semiautomatizadas. Na coleta, por sua vez, foram realizadas coletas de sangue venoso, tempo de coagulação e sangria. Conclusão: O estágio em análises clínicas se mostrou de grande importância ao graduando em farmácia, uma vez que, possibilitou por em prática os conhecimentos teóricos anteriormente adquiridos.Descritores: Técnicas de Laboratório Clínico; Laboratórios Hospitalares; Apoio ao desenvolvimento de Recursos Humanos.ReferênciasUFCG. Universidade Federal de Campina Grande. Resolução nº: 08/2009. 2009. Disponível em:<http://www.ufcg.edu.br/~costa/resolucoes/res_16082009.pdf>.Acesso em: 22 jun. 2018.CFF. Conselho Federal de Farmácia. Formação do farmacêutico para o exercício das análises clínicas e o título de farmacêutico bioquímico. 2010. Disponível em:<http://www.cff.org.br/sistemas/geral/revista/pdf/125/016_artigo_lenira.pdf>. Acesso em: 04 ago. 2018.CRF-PR. Conselho Regional de Farmácia do Paraná. Comissão de Análises Clínicas. 2018. Disponível em:< http://www.crf-pr.org. br/site/comissao/visualizar/id/4/Analises-Clinicas> Acesso em: 22 jun. 2018.CRF-PR – Conselho Regional de Farmácia do Paraná. O Farmacêutico Bioquímico e seu papel na Assistência Farmacêutica: um caso de infecção pelo HIV-2 em Curitiba. 2013. Disponível em:< http://crfpr.org.br/uploads/noticia/13030/o_farmaceutico_bioquimico_MENCAO_HONROSA.pdf>. Acesso em: 04 ago. 2018.UFCG. Universidade Federal de Campina Grande. HUAC se torna o primeiro hospital pública de Campina Grande a possuir tomógrafo computadorizado. 2008. Disponível em:<http://www.dsc.ufcg.edu.br/~pet/jornal/julho2008/materias/ufcg.htm>. Acesso em: 25 de fevereiro de 2018.Andrade Júnior FP, Barbosa, VSA. Monitoria acadêmica em parasitologia humana: um relato de experiência. Rev Saúde Com. 2017;13(3):972-75.Mota CL, Beça HP. Análise sumária de urina de rotina: porquê e para quê?. Rev Port Med Geral Fam, 2013;29(4):244-48.Heggendornn LH, Silva NA, Cunha GA. Urinálise: a importância da sedimentoscopia em exames físico-químicos normais. REB. 2014;7(4):431-43.Altenburg FL, Biondo-Simões MLP, Santiago A. Pesquisa de sangue oculto nas fezes e correlação com alterações nas colonoscopias. Rev bras colo-proctol. 2007;27(3):304-9.Jatobá MP, Candelária PAP, Klug WA, Fang CB, Capelhuchnik P. Pesquisa de sangue oculto nas fezes e chado colonoscópico em 60 pacientes. Rev bras. Colo-proctol., 2008, 28(4):425-30.USP. Universidade de São Paulo. Coleta de sangue/flebotomia. 2017. Disponível em<https://edisciplinas.usp.br/pluginfile.php/2877661/mod_resource/content/1/Coleta%20de%20Sangue%202017.pdf>. Acesso em: 23 jun. 2018.Berlitz FA. Controle da qualidade no laboratório clínico: alinhando melhoria de processos, confiabilidade e segurança do paciente. J Bras Patol Med Lab. 2010;46(5):353-63.Carvalhal GF, Rocha LCA, Monti PR. Urocultura e exame comum de urina: considerações sore sua coleta e interpretação. Rev AMRIGS. 2006; 50(1):59-62.Santos Filho L. Manual de microbiologia clínica. João Pessoa: Universitária; 2006.Wachino JI, Kimura K, Yamada K, Jin W, Arakawa Y. Evaluation of Disk Potentiation test using kirby-bauer disks containing high-dosage fosfomycin and glucose-6-phosphate to detect production of glutathione s-transferase responsible for fosfomycin resistance. J Clin Microbiol.2014;52(10):3827-828.ANVISA. Agência Nacional de Vigilância Sanitária. Teste de sensibilidade aos antimicrobianos. 2008. Disponível em:<http://www.anvisa.gov.br/servicosaude/controle/rede_rm/cursos/boas_praticas/modulo5/gram_negativos8.htm>. Acesso em: 04 ago. 2018.UFJF. Universidade Federal de Juiz de Fora. Técnicas de coloração comuns na bacteriologia. 2018. Disponível em:< http://www.ufjf.br/microbiologia/files/2018/04/ROTEIRO-PARA-AULAS-PR%C3%81TICAS-bacteriologia-2018-parte-03-Tecnicas-de-colora%C3%A7%C3% A3 o.pdf>. Acesso em: 24 nov. 2018.Miyake CET. Reticulócitos: da contagem manual à citometria de fluxo [monografia] Curitiba: Universidade Federal do Paraná – UFPR; 2011.Santos VM, Cunha SF de C, da Cunha DF. Velocidade de sedimentação das hemácias: utilidade e limitações. Rev Assoc Med Bras. 2000;46(3):232-36.UFSC. Universidade Federal de Santa Catarina. Guia prático para coleta de sangue. 2013. Disponível em<http://lidoc.ccb.ufsc.br/files/2013/10/Guia_de_Coleta_de_Sangue.pdf>.Acesso em: 04 ago. 2018.UFERSA. Universidade Federal Rural do Semiárido. Avaliação da hemostasia. 2009. Disponível em<http://www2.ufersa.edu.br/portal/view/uploads/setores/181/arquivos/Hemostasia2.pdf>. Acesso em: 04 ago. 2018Brasil. Ministério da Saúde. Manal de Diagnóstico Laboratorial das Coagulopatias Hereditárias e Plaquetopatias. 2010. Disponível em:< http://bvsms.saude.gov.br/bvs/publicacoes/manual_diagnostico_laboratorial_coagulopatias_plaquetopatias.pdf>. Acesso em: 05 ago. 2018.

Wilt is one the most serious soil-born fungal diseases of Chinese yam (Dioscorea polystachya Turczaninow cv. Tiegun), affecting plant production in many growing regions in Jiangxi province, China. The average annual incidence of wilt is 45-80%. In 2020, affected plants with wilt symptoms including withered and dried leaves, vascular discoloration, and brown necrotic stem lesions at the soil line or in the crown of the plant (Figure 1A-D) were collected from the Ruichang, Yongfeng and Taihe production areas (four fields per location) in Jiangxi province. A total of fifteen isolates were recovered from the infected stem tissues of Chinese yam and purified by single spore cultures on PDA growth medium. The fifteen isolates were similar in morphology so isolate JXRC11 was selected to be representative of the group. Pure fungal colonies of JXRC11were found to be round, white, with margin entire (Figure 1E). Macroconidia with 3-5 septations were straight to slightly curved, 23.8-40.3 µm in length and 2.6-3.9 µm in width, with predominantly 5-septate macroconidia on carnation leaf agar (CLA) (Figure 1F). However, neither microconidia or chlamydospores were observed on CLA. The morphological characteristics of the isolate were consistent with the description observed previously for Fusarium humuli species complex (Wang et al. 2019). To confirm morphological identification, ITS, CAM, TEF-1α, RPB1 and RPB2 were amplified using the primers ITS5/ITS4 (White et al. 1990), CL1/CL2A (O'Donnell et al. 2000), EF1/EF2 (O'Donnell et al. 1998), Fa/G2R (O'Donnell et al. 2010), and 5f2/11ar (O'Donnell et al. 2010), respectively. BLASTn analysis of the ITS sequence (GenBank accession no. MZ768912), EF-1α (MZ824669), CAM (MZ824670), RPB1 (MZ824672) and RPB2 (MZ824673) alignment showed 99.55%, 99.68%, 99.85%, 97.61% and 99.76% identity to those of F. humuli CQ1039 (MK280845, MK289570, MK289712, MK289840 and MK289724), respectively. Multilocus phylogenetic analyses showed that the sequences of ITS, CAM, EF-1α, RPB1, and RPB2 of the isolate belonged to the incarnatum clade (FIESC-33) of the F. incarnatum-equiseti species complex with an independent branch (Figure 2). Pathogenicity tests were conducted on one-month-old Chinese yam seedlings using a seedling root dip method (Li et al. 2013). The roots and rhizomes of seedlings grown to two meters in height were dipped into spore suspensions (1×106 spores/mL) of isolate JXRC11 for 30 min and then transferred into 20 cm diameter plastic pots containing steam-sterilized soil and placed in a greenhouse under 12 h photoperiod. After 15 d, the inoculated seedlings showed typical wilt symptoms similar to those observed in farm fields, whereas the control remained unaffected (Figure 1G-H). The pathogen was then re-isolated from the infected plants, the re-isolations were identified as F. humuli by sequencing EF-1α, fulfilling the Koch's postulates. It has been reported that the pathogen F. oxysporum Schlecht causes Fusarium wilt in five species of Dioscorea (Nwankiti and Arene, 1978). Moreover, at least 5 species of Fusarium were identified as a causative agent of Chinese yam wilt (Fang et al. 2020). To our knowledge, this is the first report of Fusarium wilt disease on Chinese yam caused by a member of the F. humuli in Jiangxi, China. This report will contribute to developing management strategies to control the disease.

Chinese figwort (Scrophularia ningpoensis Hemsl.) is an important annual herb and its dried root tubers are used as a traditional Chinese herbal medicine. In May 2021, a disease with stem rot symptoms on S. ningpoensis was observed at three randomly selected fields (~0.67 ha per field) in Nanchuan district (28.93°N, 107.27°E) of Chongqing, China. Disease incidence was estimated between 10% and 17% based on calculating the proportion of symptomatic plants. Initially, watery dark brown spots appeared on the epidermis of the stem. Then the spots expanded into spindle or strip shape, and the center of lesions were sunken, constricted and rotted finally (Figure 1A and Figure 1B). Leaves turned yellow and the plants wilted (Figure 1C). The infected parts of the stem broke easily and became brittle. The number of daughter buds used for reproduction was reduced by more than 24% and the production of root tubers decreased by more than 3%. Twelve stems with typical rot symptoms were sampled from the three fields for further investigation. Infested tissue fragments (4×4 mm) were surface sterilized with 75% ethanol for 30s and 2% sodium hypochlorite for 2 minutes in turn, finally, were rinsed 4 times with sterilized water. The disinfected tissue were air-dried and transferred onto potato dextrose agar (PDA) in the dark for 6 days at 25℃. The resulting fungal colonies were isolated by the single-spore isolation technique (Fang. 1998). Six different fungal colonies were isolated (X1-X6) and Koch’s postulates were conducted to verify the pathogenicity of individual isolates. The stem surfaces of 8 months old plants were sterilized with 75% ethanol for 30 s, rinsed three times with sterilized water, and stabbed with a sterilized needle. Conidial from the fungal colonies grown on PDA plate were harvested by filtration through five layers of sterilized absorbent gauze. Conidial concentration was then adjusted to 106 conidia per mL. 10 μL of conidial suspension was sprayed on stems injured with a sterile syringe. For each isolate, 6 plants were inoculated. Stems inoculated with sterilized water were used as a blank control. All plants were all put in a growth chamber at 28℃ with 75 to 80% relative humidity under a 12 h photoperiod for 15 days. The pathogenicity test was repeated once. After 13 days, the stems inoculated with X3 showed the same rot symptoms as we observed in the fields (Figure 1D) whereas the control stems remained symptomless (Figure 1E). The fungus re-isolated from the plants showing 100% symptoms had a similar morphology than X3 as described below. At the same time, the stems inoculated with X1, X2, X4, X5 and X6 showed no sign of rot. After culturing on PDA for 9 days under 25℃ in dark, isolate X3 grew all over the dish with white or pale pink pigmentation in the center (Figure 1F). Macroconidia were produced on synthetic low nutrient agar (SNA) plates, which showed sickle or spindle, 3 septate, straight to slightly curved with a foot-shaped basal cell, ranging from 17.595~44.88 × 2.04~3.315 μm (n=30). Microconidia were oval, elliptical or reniform, 0 to 1 septate, 3.06~12.75 ×1.785~2.805 μm (n=30) in size (Figure 1G). Phialides of conidiophores were cylindrical, short and monophialides or polyphialides (Figure 1H). Chlamydospores were found terminal or cluster with round or oblong (Figure 1I). These morphological characteristics described as Fusarium commone (Skovgaard et al. 2003). For molecular identification, the ribosomal internal transcribed spacer (ITS), translation elongation factor 1-alpha (EF-1α), RNA polymerase II subunit 1 (RPB1), the largest subunit of RNA polymerase Ⅱ gene sequences (RPB2) and the mitochondrial small subunit rDNA (mtSSU) genes were amplified with primers V9G /ITS4 (Hoog et al. 1998; White et al. 1990), EF1-668F /EF1-1251R (Alves et al. 2008), Fa/G2R (O’Donnell et al. 2010), 5f2/7cr (Liu et al. 1999; O’Donnell et al. 2010) and NMS1/NMS2 (Li et al. 1994). The sequences of isolate X3 were deposited in GenBank (MZ571935 (ITS), MZ576201 (EF-1α), MZ882396 (RPB1), MZ882397 (RPB2) and MZ867716 (mtSSU)). All sequences were revealed more than 99.8% sequence identity with reported sequences of Fusarium commune (GenBank accession No: KY630717, JF740838, KU171680, KU171700 and MK439851). Based on the optimal nucleotide replacement model SYM of multi-gene series sequence matrix, the system development tree was constructed. Results showed the strain X3 and those of F. commune (Isolates numbers were NRRL 28387, MRC 2566, MRC 2564 and CZ3-5-6) were clustered into the same evolutionary branch with a post-mortem probability of 0.996 (Figure 2). According to the morphology, molecular identification and phylogenetic analysis based on the concatenated of EF-1α and RPB2 genes sequences, the isolated X3 was identified as F. commune. The ITS sequences of X1, X2, X4, X5 and X6 showed homology exceeding 97.1% to Fusarium tricinctum (MH931273), Plectosphaerella cucumerina (MH858371), Sordariomycetes sp. (JX179237), Whalleya microplace (EF026129) and Pestalotiopsis maculiformans (EU552147), respectively, suggested the five strains to be these species possibly. GeneBank accession number of X1, X2, X4, X5 and X6 was OM074010, OM074011, OM074013, OM074015 and OM074018, respectively. To our best knowledge, this is the first report of F. commune infecting S. ningpoensis in China. Stem rot caused by F. commune is a severe threat to Chinese figwort cultivation, and identification of this pathogen is important for effective disease management and control.

Red bayberry (Myrica rubra (Lour.) Siebold & Zucc.), small trees, evergreen, dioecious, natively grows in evergreen broad-leaved forests at elevation of 1580-1875 m a.s.l., and can survive in low nutrient soil at Cao Ma Po commune, Quan Ba district, Ha Giang province. Some data on morphology, phenology, population structure, natural regeneration and distribution of Red baybery, climatic characteristics, physical and chemical properties of soil, and vegetation structure of forests having Myrica rubra occurrence are presented in this paper. Keywords Red bayberry, Myrica rubra, biology, ecology, conservation, Ha Giang, Vietnam References [1] Lu A. & Bornstein A. J., Myricaceae in Wu Z. Y. & Raven P. H. (eds.). Flora of China Vol. 4, Science Press & Missouri Botanical Garden Press, Beijing & St. Louis, 1999, pp. 275-276.[2] He X. H., Chen L. 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T., Selection of fire-resistant Tree Species for Southwestern China, Forestry Studies in China, 3(2), 2001, pp. 32-38.[38] Deng C. N., Pan X. M., Zhang H. Y. & Pan X. L., Fire-resistance of six tree species to fire probed by chlorophyll fluorescence, Journal of Food, Agriculture & Environment, 10(2), 2012, pp. 1329-1333.[39] Nguyễn Tiến Bân (Chủ biên), Danh lục các loài Thực vật Việt Nam, tập 2, Nxb. Nông nghiệp, 2003, 1203 tr.[40] Nguyễn Tiến Bân (Chủ biên), Danh lục các loài Thực vật Việt Nam, tập 3, Nxb. Nông nghiệp, 2005, 1248 tr.

Lincolnshire, England. The crowd cheer when the ball breaks loose. From one end of the field to the other, the players chase, their snouts hovering just above the grass. It’s not a case of four legs being better, rather a novel way to attract customers to the Woodside Wildlife and Falconry Park. During the matches, volunteers are drawn from the crowd to hold goal posts at either end of the run the pigs usually race on. With five pigs playing, two teams of two and a referee, and a ball designed to leak feed as it rolls (Stevenson) the ten-minute competition is fraught with tension. While the pig’s contributions to “the beautiful game” (Fish and Pele 7) have not always been so obvious, it could be argued that specific parts of the animal have had a significant impact on a sport which, despite calls to fall into line with much of the rest of the world, people in Australia (and the US) are more likely to call soccer. The Football Precursors to the modern football were constructed around an inflated pig’s bladder (Price, Jones and Harland). Animal hide, usually from a cow, was stitched around the bladder to offer some degree of stability, but the bladder’s irregular and uneven form made for unpredictable movement in flight. This added some excitement and affected how ball games such as the often violent, calico matches in Florence, were played. In the early 1970s, the world’s oldest ball was discovered during a renovation in Stirling Castle, Scotland. The ball has a pig’s bladder inside its hand-stitched, deer-hide outer. It was found in the ceiling above the bed in, what was then Mary Queens of Scots’ bedroom. It has since been dated to the 1540s (McGinnes). Neglected and left in storage until the late 1990s, the ball found pride of place in an exhibition in the Smiths Art Gallery and Museum, Stirling, and only gained worldwide recognition (as we will see later) in 2006. Despite confirmed interest in a number of sports, there is no evidence to support Mary’s involvement with football (Springer). The deer-hide ball may have been placed to gather and trap untoward spirits attempting to enter the monarch’s sleep, or simply left by accident and forgotten (McGinnes in Springer). Mary, though, was not so fortunate. She was confined and forgotten, but only until she was put to death in 1587. The Executioner having gripped her hair to hold his prize aloft, realised too late it was a wig and Mary’s head bounced and rolled across the floor. Football Development The pig’s bladder was the central component in the construction of the football for the next three hundred years. However, the issue of the ball’s movement (the bounce and roll), the bladder’s propensity to burst when kicked, and an unfortunate wife’s end, conspired to push the pig from the ball before the close of the nineteenth-century. The game of football began to take its shape in 1848, when JC Thring and a few colleagues devised the Cambridge Rules. This compromised set of guidelines was developed from those used across the different ‘ball’ games played at England’s elite schools. The game involved far more kicking, and the pig’s bladders, prone to bursting under such conditions, soon became impractical. Charles Goodyear’s invention of vulcanisation in 1836 and the death of prestigious rugby and football maker Richard Lindon’s wife in 1870 facilitated the replacement of the animal bladder with a rubber-based alternative. Tragically, Mr Lindon’s chief inflator died as a result of blowing up too many infected pig’s bladders (Hawkesley). Before it closed earlier this year (Rhoads), the US Soccer Hall of Fame displayed a rubber football made in 1863 under the misleading claim that it was the oldest known football. By the late 1800s, professional, predominantly Scottish play-makers had transformed the game from its ‘kick-and-run’ origins into what is now called ‘the passing game’ (Sanders). Football, thanks in no small part to Scottish factory workers (Kay), quickly spread through Europe and consequently the rest of the world. National competitions emerged through the growing need for organisation, and the pig-free mass production of balls began in earnest. Mitre and Thomlinson’s of Glasgow were two of the first to make and sell their much rounder balls. With heavy leather panels sewn together and wrapped around a thick rubber inner, these balls were more likely to retain shape—a claim the pig’s bladder equivalent could not legitimately make. The rubber-bladdered balls bounced more too. Their weight and external stitching made them more painful to header, but also more than useful for kicking and particularly for passing from one player to another. The ball’s relatively quick advancement can thereafter be linked to the growth and success of the World Cup Finals tournament. Before the pig re-enters the fray, it is important to glance, however briefly, at the ball’s development through the international game. World Cup Footballs Pre-tournament favourites, Spain, won the 2010 FIFA World Cup, playing with “an undistorted, perfectly spherical ball” (Ghosh par. 7), the “roundest” ever designed (FIFA par.1). Their victory may speak to notions of predictability in the ball, the tournament and the most lucrative levels of professional endeavour, but this notion is not a new one to football. The ball’s construction has had an influence on the way the game has been played since the days of Mary Queen of Scots. The first World Cup Final, in 1930, featured two heavy, leather, twelve-panelled footballs—not dissimilar to those being produced in Glasgow decades earlier. The players and officials of Uruguay and Argentina could not agree, so they played the first half with an Argentine ball. At half-time, Argentina led by two goals to one. In the second half, Uruguay scored three unanswered goals with their own ball (FIFA). The next Final was won by Italy, the home nation in 1934. Orsi, Italy’s adopted star, poked a wildly swerving shot beyond the outstretched Czech keeper. The next day Orsi, obligated to prove his goal was not luck or miracle, attempted to repeat the feat before an audience of gathered photographers. He failed. More than twenty times. The spin on his shot may have been due to the, not uncommon occurrence, of the ball being knocked out of shape during the match (FIFA). By 1954, the Federation Internationale de Football Association (FIFA) had sought to regulate ball size and structure and, in 1958, rigorously tested balls equal to the demands of world-class competition. The 1950s also marked the innovation of the swerving free kick. The technique, developed in the warm, dry conditions of the South American game, would not become popular elsewhere until ball technology improved. The heavy hand-stitched orb, like its early counterparts, was prone to water absorption, which increased the weight and made it less responsive, particularly for those playing during European winters (Bray). The 1970 World Cup in Mexico saw football progress even further. Pele, arguably the game’s greatest player, found his feet, and his national side, Brazil, cemented their international football prominence when they won the Jules Rimet trophy for the third time. Their innovative and stylish use of the football in curling passes and bending free kicks quickly spread to other teams. The same World Cup saw Adidas, the German sports goods manufacturer, enter into a long-standing partnership with FIFA. Following the competition, they sold an estimated six hundred thousand match and replica tournament footballs (FIFA). The ball, the ‘Telstar’, with its black and white hexagonal panels, became an icon of the modern era as the game itself gained something close to global popularity for the first time in its history. Over the next forty years, the ball became incrementally technologically superior. It became synthetic, water-resistant, and consistent in terms of rebound and flight characteristics. It was constructed to be stronger and more resistant to shape distortion. Internal layers of polyutherane and Syntactic Foam made it lighter, capable of greater velocity and more responsive to touch (FIFA). Adidas spent three years researching and developing the 2006 World Cup ball, the ‘Teamgeist’. Fourteen panels made it rounder and more precise, offering a lower bounce, and making it more difficult to curl due to its accuracy in flight. At the same time, audiences began to see less of players like Roberto Carlos (Brazil and Real Madrid CF) and David Beckham (Manchester United, LA Galaxy and England), who regularly scored goals that challenged the laws of physics (Gill). While Adidas announced the 2006 release of the world’s best performing ball in Berlin, the world’s oldest was on its way to the Museum fur Volkerkunde in Hamburg for the duration of the 2006 FIFA World Cup. The Mary Queen of Scot’s ball took centre spot in an exhibit which also featured a pie stand—though not pork pies—from Hibernian Football Club (Strang). In terms of publicity and raising awareness of the Scots’ role in the game’s historical development, the installation was an unrivalled success for the Scottish Football Museum (McBrearty). It did, however, very little for the pig. Heads, not Tails In 2002, the pig or rather the head of a pig, bounced and rolled back into football’s limelight. For five years Luis Figo, Portugal’s most capped international player, led FC Barcelona to domestic and European success. In 2000, he had been lured to bitter rivals Real Madrid CF for a then-world record fee of around £37 million (Nash). On his return to the Catalan Camp Nou, wearing the shimmering white of Real Madrid CF, he was showered with beer cans, lighters, bottles and golf balls. Among the objects thrown, a suckling pig’s head chimed a psychological nod to the spear with two sharp ends in William Golding’s story. Play was suspended for sixteen minutes while police tried to quell the commotion (Lowe). In 2009, another pig’s head made its way into football for different reasons. Tightly held in the greasy fingers of an Orlando Pirates fan, it was described as a symbol of the ‘roasting’ his team would give the Kaiser Chiefs. After the game, he and his friend planned to eat their mascot and celebrate victory over their team’s most reviled competitors (Edwards). The game ended in a nil-all draw. Prior to the 2010 FIFA World Cup, it was not uncommon for a range of objects that European fans might find bizarre, to be allowed into South African league matches. They signified luck and good feeling, and in some cases even witchcraft. Cabbages, known locally for their medicinal qualities, were very common—common enough for both sets of fans to take them (Edwards). FIFA, an organisation which has more members than the United Nations (McGregor), impressed their values on the South African Government. The VuVuZela was fine to take to games; indeed, it became a cultural artefact. Very little else would be accepted. Armed with their economy-altering engine, the world’s most watched tournament has a tendency to get what it wants. And the crowd respond accordingly. Incidentally, the ‘Jabulani’—the ball developed for the 2010 tournament—is the most consistent football ever designed. In an exhaustive series of tests, engineers at Loughborough University, England, learned, among other things, the added golf ball-like grooves on its surface made the ball’s flight more symmetrical and more controlled. The Jabulani is more reliable or, if you will, more predictable than any predecessor (Ghosh). Spanish Ham Through support from their Governing body, the Real Federación Española de Fútbol, Spain have built a national side with experience, and an unparalleled number of talented individuals, around the core of the current FC Barcelona club side. Their strength as a team is founded on the bond between those playing on a weekly basis at the Catalan club. Their style has allowed them to create and maintain momentum on the international stage. Victorious in the 2008 UEFA European Football Championship and undefeated in their run through the qualifying stages into the World Cup Finals in South Africa, they were tournament favourites before a Jabulani was rolled into touch. As Tim Parks noted in his New York Review of Books article, “The Shame of the World Cup”, “the Spanish were superior to an extent one rarely sees in the final stages of a major competition” (2010 par. 15). They have a “remarkable ability to control, hold and hide the ball under intense pressure,” and play “a passing game of great subtlety [ ... to] patiently wear down an opposing team” (Parks par. 16). Spain won the tournament having scored fewer goals per game than any previous winner. Perhaps, as Parks suggests, they scored as often as they needed to. They found the net eight times in their seven matches (Fletcher). This was the first time that Spain had won the prestigious trophy, and the first time a European country has won the tournament on a different continent. In this, they have broken the stranglehold of superpowers like Germany, Italy and Brazil. The Spanish brand of passing football is the new benchmark. Beautiful to watch, it has grace, flow and high entertainment value, but seems to lack something of an organic nature: that is, it lacks the chance for things to go wrong. An element of robotic aptitude has crept in. This occurred on a lesser scale across the 2010 FIFA World Cup finals, but it is possible to argue that teams and players, regardless of nation, have become interchangeable, that the world’s best players and the way they play have become identikits, formulas to be followed and manipulated by master tacticians. There was a great deal of concern in early rounds about boring matches. The world’s media focused on an octopus that successfully chose the winner of each of Germany’s matches and the winner of the final. Perhaps, in shaping the ‘most’ perfect ball and the ‘most’ perfect football, the World Cup has become the most predictable of tournaments. In Conclusion The origins of the ball, Orsi’s unrepeatable winner and the swerving free kick, popular for the best part of fifty years, are worth remembering. These issues ask the powers of football to turn back before the game is smothered by the hunt for faultlessness. The unpredictability of the ball goes hand in hand with the game. Its flaws underline its beauty. Football has so much more transformative power than lucrative evolutionary accretion. While the pig’s head was an ugly statement in European football, it is a symbol of hope in its South African counterpart. Either way its removal is a reminder of Golding’s message and the threat of homogeneity; a nod to the absence of the irregular in the modern era. Removing the curve from the free kick echoes the removal of the pig’s bladder from the ball. The fun is in the imperfection. Where will the game go when it becomes indefectible? Where does it go from here? Can there really be any validity in claiming yet another ‘roundest ball ever’? Chip technology will be introduced. The ball’s future replacements will be tracked by satellite and digitally-fed, reassured referees will determine the outcome of difficult decisions. Victory for the passing game underlines the notion that despite technological advancement, the game has changed very little since those pioneering Scotsmen took to the field. Shouldn’t we leave things the way they were? Like the pigs at Woodside Wildlife and Falconry Park, the level of improvement seems determined by the level of incentive. The pigs, at least, are playing to feed themselves. Acknowledgments The author thanks editors, Donna Lee Brien and Adele Wessell, and the two blind peer reviewers, for their constructive feedback and reflective insights. The remaining mistakes are his own. References “Adidas unveils Golden Ball for 2006 FIFA World Cup Final” Adidas. 18 Apr. 2006. 23 Aug. 2010 . Bray, Ken. “The science behind the swerve.” BBC News 5 Jun. 2006. 19 Aug. 2010 http://news.bbc.co.uk/2/hi/uk_news/magazine/5048238.stm>. Edwards, Piers. “Cabbage and Roasted Pig.” BBC Fast Track Soweto, BBC News 3 Nov. 2009. 23 Aug. 2010 . FIFA. “The Footballs during the FIFA World Cup™” FIFA.com. 18 Aug. 2010 .20 Fish, Robert L., and Pele. My Life and the Beautiful Game. New York: Bantam Dell, 1977. Fletcher, Paul. “Match report on 2010 FIFA World Cup Final between Spain and Netherlands”. BBC News—Sports 12 Jul. 2010 . Ghosh, Pallab. “Engineers defend World Cup football amid criticism.” BBC News—Science and Environment 4 Jun. 2010. 19 Aug. 2010 . Gill, Victoria. “Roberto Carlos wonder goal ‘no fluke’, say physicists.” BBC News—Science and Environment 2 Sep. 2010 . Hawkesley, Simon. Richard Lindon 22 Aug. 2010 . “History of Football” FIFA.com. Classic Football. 20 Aug. 2010 . Kay, Billy. The Scottish World: A Journey into the Scottish Diaspora. London: Mainstream, 2008. Lowe, Sid. “Peace for Figo? And pigs might fly ...” The Guardian (London). 25 Nov. 2002. 20 Aug. 2010 . “Mary, Queen of Scots (r.1542-1567)”. The Official Website of the British Monarchy. 20 Jul. 2010 . McBrearty, Richard. Personal Interview. 12 Jul. 2010. McGinnes, Michael. Smiths Art Gallery and Museum. Visited 14 Jul. 2010 . McGregor, Karen. “FIFA—Building a transnational football community. University World News 13 Jun. 2010. 19 Jul. 2010 . Nash, Elizabeth. “Figo defects to Real Madrid for record £36.2m." The Independent (London) 25 Jul. 2000. 20 Aug. 2010 . “Oldest football to take cup trip” 25 Apr. 2006. 20 Jul. 2010 . Parks, Tim. “The Shame of the World Cup”. New York Review of Books 19 Aug. 2010. 23 Aug. 2010 < http://nybooks.com/articles/archives/2010/aug/19/shame-world-cup/>. “Pig football scores a hit at centre.” BBC News 4 Aug. 2009. August 20 2010 . Price, D. S., Jones, R. Harland, A. R. “Computational modelling of manually stitched footballs.” Proceedings of the Institution of Mechanical Engineers, Part L. Journal of Materials: Design & Applications 220 (2006): 259-268. Rhoads, Christopher. “Forget That Trip You Had Planned to the National Soccer Hall of Fame.” Wall Street Journal 26 Jun. 2010. 22 Sep. 2010 . “Roberto Carlos Impossible Goal”. News coverage posted on You Tube, 27 May 2007. 23 Aug. 2010 . Sanders, Richard. Beastly Fury. London: Bantam, 2009. “Soccer to become football in Australia”. Sydney Morning Herald 17 Dec. 2004. 21 Aug. 2010 . Springer, Will. “World’s oldest football – fit for a Queen.” The Scotsman. 13 Mar. 2006. 19 Aug. 2010 < http://heritage.scotsman.com/willspringer/Worlds-oldest-football-fit.2758469.jp >. Stevenson, R. “Pigs Play Football at Wildlife Centre”. Lincolnshire Echo 3 Aug. 2009. 20 Aug. 2010 . Strang, Kenny. Personal Interview. 12 Jul. 2010. “The Execution of Mary Queen of Scots February 8, 1857”. Tudor History 21 Jul. 2010 http://tudorhistory.org/primary/exmary.html>. “The History of the FA.” The FA. 20 Jul. 2010 “World’s Oldest Ball”. World Cup South Africa 2010 Blog. 22 Jul. 2010 . “World’s Oldest Soccer Ball by Charles Goodyear”. 18 Mar. 2010. 20 Jul. 2010 .

Background: The optimal antithrombotic regimen for patients with coexistent atrial fibrillation (AF) and coronary artery disease (CAD) requiring percutaneous coronary intervention (PCI) remains controversial.Methods: We performed a chart review of 2,645 consecutive patients with non-ST elevation or ST elevation myocardial infarction at a regional cardiac centre, to examine the clinical characteristics and discharge antithrombotic medications of patients with coexistent AF (known or new onset AF with CHADS2 ≥1), treated with PCI.Results: Among 2,645 patients, 94 eligible patients were analyzed and 30 (32%) were prescribed triple therapy (TT) at hospital discharge. CHADS2 score was the major predictor of the decision to prescribe TT (P =0.002).Conclusion: Approximately one-third of the patients with AF undergoing PCI were prescribed TT at hospital discharge. Clinicians are generally following national guidelines and internationally-developed consensus statements, and focus on stroke risk despite the risks of bleeding and insufficient evidence supporting the benefits of TT. RésuméContexte : Le régime optimal antithrombotique pour les patients atteints à la fois de fibrillation atriale (FA) et d’une coronaropathie nécessitant une angioplastie coronarienne percutanée (ACP) demeure controversé.Méthodologie: Dans un centre régional de cardiologie, nous avons examiné les dossiers médicaux de 2645 patients ayant subi un infarctus du myocarde avec ou sans sus‑décalage du segment ST. Le but consistait à analyser les caractéristiques cliniques des patients présentant une FA concomitante (ancienne ou nouvelle, avec un indice CHADS2 ≥ 1) traitée par ACP, et les médicaments antithrombotiques prescrits au moment de leur sortie de l’hôpital.Résultats: Parmi les 2645 dossiers, 94 patients admissibles ont été analysés. Trente (32 %) s’étaient vus prescrire une trithérapie (TT) à leur sortie de l’hôpital. L’indice CHADS2 était le principal indicateur prévisionnel pris en compte dans la décision de prescrire la TT (P = 0,002).Conclusion : Environ un tiers des patients atteints de FA et ayant subi une ACP se sont vus prescrire une TT à leur sortie de l’hôpital. En général, les cliniciens suivent les directives nationales et les protocoles consensuels élaborés à l’international et se concentrent sur le risque d’ictus, malgré les risques de saignements et le manque de données probantes soutenant les avantages d’une TT.BACKGROUNDDual antiplatelet therapy (DAPT) including Aspirin and a P2Y12 receptor antagonist is recommended for patients who undergo percutaneous coronary intervention (PCI) with stent implantation.1,2 DAPT is superior to oral anticoagulants (OAC) in preventing stent thrombosis, 3while OAC is superior to DAPT in reducing stroke in patients with atrial fibrillation (AF).4,5 It is estimated that 5–8% of patients sustaining an acute coronary syndrome (ACS) have concomitant AF.6,7 The optimal antithrombotic regimen for patients with coexistent AF and coronary artery disease (CAD) requiring PCI remains controversial. In patients with AF and CHADS2 stroke risk score ≥1, consensus statements suggest that triple therapy (TT), defined as a combination of DAPT + OAC, be utilized in patients undergoing PCI with ACS.8,9 However, there is data indicating no improved efficacy of treatment with TT compared with DAPT alone, while exposing patients to increased bleeding risk.7,10,11 Major bleeding has been proven to be independently associated with death in patients following an ACS.12 Therefore, balancing the thromboembolic and bleeding risk is critical in patients with a recent ACS.Large randomized trials have indicated that compared with warfarin, novel oral anticoagulants (NOAC’s) are at least as effective as, and are associated with reduced rates of major, fatal and intracranial bleeding in patients with non-valvular AF.13 Due to the favourable safety profile, NOACs are being evaluated against warfarin in patients with AF undergoing PCI. Three of these trials are ongoing (RE-DUAL PCI, AUGUSTUS, ENTRUST-AF-PCI),14–16 and the fourth is the recently published PIONEER AF-PCI trial, which showed that reduced-dose rivaroxaban combined with clopidogrel lowered the risk of bleeding compared with TT with warfarin.17Despite the emergence of these recent data, the highest risk patients remain excluded from large randomized trials, and thus sound clinical judgment will remain the cornerstone in caring for these patients. In the current study, we aim to describe the local practice patterns of clinicians making treatment decisions for patients with new or existing AF, who present with an ACS and undergo PCI.METHODSA retrospective and prospective chart review was performed on 2,645 consecutive patients presenting with ST elevation myocardial infarction (STEMI) and non-ST elevation myocardial elevation (NSTEMI) to 3 academic hospitals affiliated with McMaster University and 8 community hospitals located in Local Health Integrated Network (LHIN) 4, Ontario, Canada from January to December 2014. Of those, 1,458 patients had undergone PCI with stent implantation; and, 124 patients were identified to have concomitant AF (Figure 1). Inclusion criteria for our study were: admission to hospital for either STEMI or NSTEMI, known or new onset AF with CHADS2 ≥ 1, coronary angiography demonstrating at least one epicardial coronary artery with a ≥70% stenosis, and successful PCI with stent implantation. Patients who expired before discharge were excluded from the analysis. Clinical and demographic characteristics of patients were summarized. DAPT and TT groups were identified by reviewing medication records from discharge summaries or copies of discharge prescriptions. DAPT was defined as Aspirin plus clopidogrel (75 mg once daily) or ticagrelor (90 mg twice daily), while TT was defined as DAPT plus an OAC; including either warfarin or novel oral anticoagulants (NOAC). We analyzed the CHADS2 and ATRIA (Anticoagulation and Risk factors in Atrial fibrillation) scores according to treatment groups.18,19 Statistical analysis was performed using SAS/STAT version 9.3 (SAS Institute Inc., Cary, NC). Categorical data was compared using Chi-square testing. Continuous data comparison was performed applying the Wilcoxon test and Cochran-Armitage test for trends. A binary logistic regression model was developed to identify the clinical predictors of discharge groups based on their CHADS 2 and ATRIA scores (Figure 2 and Figure 3). The cut-off for statistical significance was a P-value of <0.05.Figure 1. Flow chart of study cohort. Figure 2. Use of triple therapy versus dual antiplatelet therapy at the time of discharge in different stroke risk groups predicted by their CHADS2 score. Figure 3. Use of triple therapy versus dual antiplatelet therapy at the time of discharge in different bleeding risk groups predicted by their ATRIA score. RESULTSDemographic and clinical characteristics of patients are described in Table 1. Ninety four out of 124 patients with ACS and a prior or new diagnosis of AF were included in the final analysis. The mean (s.d.) age was 74 years (10 years) and 55.3% were male. In our cohort, 59.6% of patients sustained a STEMI and 54.3% had single vessel CAD. Overall, 32% (n = 30) of patients were discharged on TT of whom 55.6% were prescribed a combination of DAPT and a NOAC while the remaining patients were prescribed conventional TT (DAPT+warfarin) (Table 2). The median CHADS2 score in the DAPT and TT groups were 2 and 3, respectively (P = 0.001). The median ATRIA bleeding risk in DAPT and TT groups were 3 and 4, respectively (P = 0.008). The P-value for trend of CHADS 2 and ATRIA scores were <0.0001 and 0.001, respectively. However, these 2 trends were not significantly different from one another (P = 0.26). In a logistic regression analysis with both CHADS 2 and ATRIA scores included in the model, the CHADS2 score was significantly different between both groups (P = 0.002), while the ATRIA score was not (P = 0.58). In patients who were prescribed TT, 36.6% had a history of stroke or TIA compared with 10.9% in the DAPT group (P = 0.005). Additionally, the patients who were discharged on TT were more likely to have been treated with an OAC prior to admission, compared with those in the DAPT group (70% vs 17.1%; P < 0.001). We observed that there was proportionally more use of a BMS in patients with AF (41.5% of the 94 patients) compared with BMS being used in the whole cohort (15% of the 1,458 patients).There was no statistically significant difference in the likelihood of being discharged on TT based on whether patients were hospitalized at a community or academic hospital (44% vs. 56%, P = 0.42). Academic hospitals had the availability of a thrombosis service and they provided a consultation on 18% of the patients analyzed at those hospitals. The thrombosis service was generally involved in the care of patients with higher CHADS2 scores,and the involvement of the thrombosis service was associated with a significantly increased use of TT (P = 0.002). DISCUSSIONOur results indicate that approximately one-third of patients were prescribed TT at hospital discharge, while the remaining patients received DAPT alone. No patients received a combination of single antiplatelet therapy and an OAC. Although both the CHADS2 and ATRIA scores were significantly higher in the TT group, the CHADS2 score more strongly predicted the use of TT in our local practice. Our results also showed that OAC use prior to admission increased the probability of TT use upon discharge. Furthermore, the current study reveals that in patients discharged on TT, a combination of Aspirin, a P2Y12 inhibitor and NOAC were prescribed more often than conventional TT with warfarin despite the lack of clear evidence about their safety profile. 20,21An analysis of the AVIATOR registry, which included patients with a similar mean CHADS2 score as in our study (2.7 in the AVIATOR registry vs. 2.3 in our cohort) reported that 41.2% of patients were prescribed TT at discharge,22 compared with 31.9% in our cohort. This indicates that some practice variability exists among clinicians when considering treatment with TT in patients with similar risk profiles. The AVIATOR registry data also demonstrated that patients who were discharged on TT had a higher risk of stroke defined by their CHADS2 score but in contrast to our results, their bleeding risk score was not higher than the DAPT group. Our results were, however, consistent with another retrospective study within a large registry, which reported greater use of OAC at discharge among patients with both higher stroke and bleeding risk. 23 This observation highlights the important point that many major predictors of stroke, such as advanced age, are also important risk factors for bleeding.TT in our local clinical practice was used more selectively and was predominantly for those patients with a high CHADS2 score (≥3). Our data also show that clinicians favoured use of TT even in patients with higher bleeding risk despite the lack of robust evidence.24Our study was designed to describe practice patterns only, and therefore, one limitation was the inability to report patient outcomes following hospital discharge. Secondly, given the observational and retrospective nature of our study and the relatively small study cohort we are only able to describe associations. Thirdly, there are unmeasured variables such as frailty, patients’ overall goals of care, and personal preferences that could contribute to the ultimate decision for a given antithrombotic regimen, which were not captured in our study. Fourthly, the type and dose of NOAC were not collected in our study. Our study was unique in assessing practice parameters such as discharge settings (academic versus community hospitals) and describing the effect of the involvement of a thrombosis service in making treatment decisions.The choice of the most favourable antithrombotic regimen in patients with AF and ACS undergoing PCI remains an area of clinical debate. There were no patients in our study cohort discharged on a combination of a P2Y 12 inhibitor and warfarin alone, a regimen evaluated in the previously published WOEST trial.25 This observation indicates either a knowledge gap or reluctance among clinicians in applying the results of the WOEST study. The results of the PIONEER AF-PCI trial suggest that a WOEST study type strategy of reduced-dose rivaroxaban with single antiplatelet therapy (clopidogrel) is an attractive alternative to warfarin because of the substantial reduction in major bleeding. This trial has been criticized because it was not powered for ischemic events, had an open-label design, and tested doses of rivaroxaban that had not been previously evaluated.26 However, PIONEER AF-PCI and ongoing trials in this area will hopefully strengthen the body of evidence and lead to more informed clinical decision making and improved patient care. Despite the growing evidence in this area, the uptake will remain slow among clinicians until emergence of further robust evidence and variability of practice will persist as partially reflected in the current study.DisclosureThere was no dedicated funding for this study.None of the authors have any disclosures relevant to this study.REFERENCES1. 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