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Journal articles on the topic 'E. coli 0104:H4'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Bamaiyi, P.H. "EMERGING FOOD PATHOGENS: A CASE STUDY OF E. Coli 0104:H4." Continental J. Animal and Veterinary Research 3, no. 2 (2011): 21–27. https://doi.org/10.5281/zenodo.818062.

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Food is essential for the survival of man and animals in the planet but this necessity sometimes becomes the means of destruction from the planet. Most infections that man get are either from food or water. In the last 20 years there have been several cases of food borne pathogens causing deleterious infections in man and animals. Some of these pathogens are just newly emerging and not much has been studied on them until recent times. The bulk of pathogens of food are of bacterial origin. In this review we looked at current trends in emerging food pathogens globally as it concerns the health o

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2

Wiwanitkit, Viroj. "Hemolysis in E. coli O104:H4 Infection." Indian Journal of Hematology and Blood Transfusion 28, no. 2 (2011): 127. http://dx.doi.org/10.1007/s12288-011-0109-8.

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Bamaiyi, P.H. "PERSPECTIVES ON INTEGRATED APPROACH TO FOOD SAFETY." Continental J. Animal and Veterinary Research 3, no. 2 (2011): 28–35. https://doi.org/10.5281/zenodo.818064.

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Food is universal to man as well as animals which also provide a bulk of the food needed by man. The current population of the world stands at about 7 billion. With this increasing population is also the increasing need to provide not just food but also healthy food for the consumption of man. In this paper we examine the integrated approach to food safety in order to meet the need for healthy food by man and in a healthy manner to all the occupants of the planet whether plants or animals. This is even more important considering new trends in outbreaks of food-borne diseases around the world s

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Aller Gancedo, Benito M., Miguel Cordero del Campillo, and Antonio R. Martínez Fernández. "Características bioquímicas y serológicas de algunas cepas de Escherichia coli procedentes de gallina." Anales de la Facultad de Veterinaria de León 14 (May 29, 2025): 207–13. https://doi.org/10.18002/analesdeveterinaria.v14.8925.

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Se estudian bioquímica y serológicamente 31 ce`pas de E. coli, aisladas a partir de cadáveres de gallinas. Sorbita, rafinosa y sacarosa las fermentaron el 83, 74 y 67% de las cepas. Se determinaron veinte serotipos 0, siendo las más frecuentes los 0190, 02 y 0111. De los antígenos flagelares, los hallados fueron diez, con la máxima frecuencia para los H10 y H4. En cuanto a la correspondencia entre antígenos somáticos y flagelares, la más frecuente fue la fórmula 0109:H10 (cinco cepas).

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NAGAOKA, HIROMI, SHINICHIRO HIRAI, HIROTAKA MORINUSHI, et al. "Coinfection with Human Norovirus and Escherichia coli O25:H4 Harboring Two Chromosomal blaCTX-M-14 Genes in a Foodborne Norovirus Outbreak in Shizuoka Prefecture, Japan." Journal of Food Protection 83, no. 9 (2020): 1584–91. http://dx.doi.org/10.4315/jfp-20-042.

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ABSTRACT Hospital-acquired infections caused by extended-spectrum β-lactamase (ESBL)–producing Escherichia coli are a global problem. Healthy people can carry ESBL-producing E. coli in the intestines; thus, E. coli from healthy people can potentially cause hospital-acquired infections. Therefore, the transmission routes of ESBL-producing E. coli from healthy persons should be determined. A foodborne outbreak of human norovirus (HuNoV) GII occurred at a restaurant in Shizuoka, Japan, in 2018. E. coli O25:H4 was isolated from some of the HuNoV-infected customers. Pulsed-field gel electrophoresis

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Makarova, Mariia A., Zoya N. Matveeva, E. V. Smirnova, et al. "LABORATORY ERRORS FOR IDENTIFICATION OF THE ESCHERICHIA COLI STRAINS OF THE SEROLOGICAL GROUPS O6 AND O25 AS ACUTE INTESTINAL INFECTIONS." Russian Clinical Laboratory Diagnostics 65, no. 6 (2020): 368–74. http://dx.doi.org/10.18821/0869-2084-2020-65-6-368-374.

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Were studied the genes encoding the virulence factors of 221 strains: E. coli O6:H1 (194) and E. coli O25:H4 (27), isolated in 2014-2018 from stool samples of children and adults examined according to epidemic indications. Molecular methods included PCR with hybridization-fluorescence and electrophoresis detection of amplified products. The strains did not have virulence genes for diarrheagenic E. coli (DEC) pathogroups EPEC, ETEC, EIEC, EHEC, EAggEC, and belonged to the phylogenetic group B2. They contained from four to eight genes encoding virulence factors of ExPEC: E. coli O6:H1 - pap (68,

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7

Amemiya, Kei, David A. Rozak, Jennifer L. Dankmeyer, et al. "Shiga-Toxin-Producing Strains of Escherichia coli O104:H4 and a Strain of O157:H7, Which Can Cause Human Hemolytic Uremic Syndrome, Differ in Biofilm Formation in the Presence of CO2 and in Their Ability to Grow in a Novel Cell Culture Medium." Microorganisms 11, no. 7 (2023): 1744. http://dx.doi.org/10.3390/microorganisms11071744.

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One pathogen that commonly causes gastrointestinal illnesses from the consumption of contaminated food is Escherichia coli O157:H7. In 2011 in Germany, however, there was a prominent outbreak of bloody diarrhea with a high incidence of hemolytic uremic syndrome (HUS) caused by an atypical, more virulent E. coli O104:H4 strain. To facilitate the identification of this lesser-known, atypical E. coli O104:H4 strain, we wanted to identify phenotypic differences between it and a strain of O157:H7 in different media and culture conditions. We found that E. coli O104:H4 strains produced considerably

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8

Böhnlein, Christina, Jan Kabisch, Diana Meske, Charles M. A. P. Franz, and Rohtraud Pichner. "Fitness of Enterohemorrhagic Escherichia coli (EHEC)/Enteroaggregative E. coli O104:H4 in Comparison to That of EHEC O157: Survival Studies in Food andIn Vitro." Applied and Environmental Microbiology 82, no. 21 (2016): 6326–34. http://dx.doi.org/10.1128/aem.01796-16.

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ABSTRACTIn 2011, one of the world's largest outbreaks of hemolytic-uremic syndrome (HUS) occurred, caused by a rareEscherichia coliserotype, O104:H4, that shared the virulence profiles of Shiga toxin-producingE. coli(STEC)/enterohemorrhagicE. coli(EHEC) and enteroaggregativeE. coli(EAEC). The persistence and fitness factors of the highly virulent EHEC/EAEC O104:H4 strain, grown either in food orin vitro, were compared with those ofE. coliO157 outbreak-associated strains. The log reduction rates of the different EHEC strains during the maturation of fermented sausages were not significantly dif

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9

Chen, Yen-Hsu, and Po-Ren Hsueh. "European Escherichia coli O104:H4 outbreak." Journal of the Formosan Medical Association 110, no. 10 (2011): 611–12. http://dx.doi.org/10.1016/j.jfma.2011.08.001.

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10

Monecke, Stefan, Patricia Mariani-Kurkdjian, Edouard Bingen, et al. "Presence of Enterohemorrhagic Escherichia coli ST678/O104:H4 in France Prior to 2011." Applied and Environmental Microbiology 77, no. 24 (2011): 8784–86. http://dx.doi.org/10.1128/aem.06524-11.

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ABSTRACTTwo isolates of enterohemorrhagicEscherichia coli(EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and to other O104:H4 strains. This suggests that different O104:H4 EHEC strains were present several years prior to the 2011 outbreak.

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Dyatlov, Ivan A., Edward A. Svetoch, Anna A. Mironenko, et al. "Molecular Lipopolysaccharide Di-Vaccine Protects from Shiga-Toxin Producing Epidemic Strains of Escherichia coli O157:H7 and O104:H4." Vaccines 10, no. 11 (2022): 1854. http://dx.doi.org/10.3390/vaccines10111854.

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Background: Shiga toxin-producing Escherichia coli (STEC) O157:H7 and O104:H4 strains are important causative agents of food-borne diseases such as hemorrhagic colitis and hemolytic–uremic syndrome, which is the leading cause of kidney failure and death in children under 5 years as well as in the elderly. Methods: the native E. coli O157:H7 and O104:H4 lipopolysaccharides (LPS) were partially deacylated under alkaline conditions to obtain apyrogenic S-LPS with domination of tri-acylated lipid A species—Ac3-S-LPS. Results: intraperitoneal immunization of BALB/c mice with Ac3-S-LPS antigens from

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12

BEUTIN, LOTHAR, and ANNETT MARTIN. "Outbreak of Shiga Toxin–Producing Escherichia coli (STEC) O104:H4 Infection in Germany Causes a Paradigm Shift with Regard to Human Pathogenicity of STEC Strains." Journal of Food Protection 75, no. 2 (2012): 408–18. http://dx.doi.org/10.4315/0362-028x.jfp-11-452.

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An outbreak that comprised 3,842 cases of human infections with enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104:H4 occurred in Germany in May 2011. The high proportion of adults affected in this outbreak and the unusually high number of patients that developed hemolytic uremic syndrome makes this outbreak the most dramatic since enterohemorrhagic E. coli (EHEC) strains were first identified as agents of human disease. The characteristics of the outbreak strain, the way it spread among humans, and the clinical signs resulting from EAHEC infections have changed the way Shiga toxin–pr

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13

Secher, T., A. Shima, K. Hinsinger, et al. "Retrograde Trafficking Inhibitor of Shiga Toxins Reduces Morbidity and Mortality of Mice Infected with Enterohemorrhagic Escherichia coli." Antimicrobial Agents and Chemotherapy 59, no. 8 (2015): 5010–13. http://dx.doi.org/10.1128/aac.00455-15.

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ABSTRACTThe most deadly outbreak ofEscherichia coliO104:H4 occurred in Europe in 2011. Here, we evaluated the effects of the retrograde trafficking inhibitor Retro-2cyclin a murine model ofE. coliO104:H4 infection. Systemic treatment with Retro-2cyclsignificantly reduced body weight loss and improved clinical scores and survival rates for O104:H4-infected mice. The present data established that Retro-2cyclcontributes to the protection of mice against O104:H4 infection and may represent a novel approach to limit Shiga toxin-producingEscherichia coli(STEC)-induced toxicity.

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14

JUNEJA, VIJAY K., SUDARSAN MUKHOPADHYAY, DIKE UKUKU, CHENG-AN HWANG, VIVIAN C. H. WU, and HARSHAVARDHAN THIPPAREDDI. "Interactive Effects of Temperature, pH, and Water Activity on the Growth Kinetics of Shiga Toxin–ProducingEscherichia coli O104:H4†." Journal of Food Protection 77, no. 5 (2014): 706–12. http://dx.doi.org/10.4315/0362-028x.jfp-13-387.

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The risk of non-O157 Shiga toxin–producing Escherichia coli strains has become a growing public health concern. Several studies characterized the behavior of E. coli O157:H7; however, no reports on the influence of multiple factors on E. coli O104:H4 are available. This study examined the effects and interactions of temperature (7 to 46°C), pH (4.5 to 8.5), and water activity (aw; 0.95 to 0.99) on the growth kinetics of E. coli O104:H4 and developed predictive models to estimate its growth potential in foods. Growth kinetics studies for each of the 23 variable combinations from a central compo

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15

Navarro, Armando, Claudia van der Ploeg, Ariel Rogé, et al. "Diversity of Potentially Pathogenic Escherichia coli O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions." Microorganisms 9, no. 11 (2021): 2227. http://dx.doi.org/10.3390/microorganisms9112227.

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In 2011, an outbreak of hemorrhagic colitis and hemolytic uremic syndrome (HUS) was reported in Europe that was related to a hybrid STEAEC of Escherichia coli (E. coli) O104:H4 strain. The current study aimed to analyze strains of E. coli O104 and O9 isolated before 2011. The study included 47 strains isolated from children with and without diarrhea between 1986 and 2009 from different geographic regions, as well as seven reference strains. Serotyping was carried out on 188 anti-O and 53 anti-H sera. PCR was used to identify DEC genes and phylogenetic groups. Resistance profiles to antimicrobi

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16

Mariani-Kurkdjian, P., and E. Bingen. "Escherichia coli O104:H4 : un pathotype hybride." Archives de Pédiatrie 19 (November 2012): S97—S100. http://dx.doi.org/10.1016/s0929-693x(12)71281-2.

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17

Jandhyala, Dakshina M., Vijay Vanguri, Erik J. Boll, YuShuan Lai, Beth A. McCormick, and John M. Leong. "Shiga Toxin–Producing Escherichia coli O104:H4." Infectious Disease Clinics of North America 27, no. 3 (2013): 631–49. http://dx.doi.org/10.1016/j.idc.2013.05.002.

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18

Giles, M., S. A. Cawthraw, M. AbuOun, et al. "Host-specific differences in the contribution of an ESBL IncI1 plasmid to intestinal colonization by Escherichia coli O104:H4." Journal of Antimicrobial Chemotherapy 73, no. 6 (2018): 1579–85. http://dx.doi.org/10.1093/jac/dky037.

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AbstractObjectivesTo assess stability and contribution of a large ESBL-encoding IncI1 plasmid to intestinal colonization by Escherichia coli O104:H4 in two different mammalian hosts.MethodsSpecific-pathogen-free 3–4-day-old New Zealand White rabbits and conventionally reared 6-week-old weaned lambs were orally infected with WT E. coli O104:H4 or the ESBL-plasmid-cured derivative, and the recovery of bacteria in intestinal homogenates and faeces monitored over time.ResultsCarriage of the ESBL plasmid had differing impacts on E. coli O104:H4 colonization of the two experimental hosts. The plasmi

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19

Kislichkina, Angelina A., Nikolay N. Kartsev, Yury P. Skryabin, et al. "Genomic Analysis of a Hybrid Enteroaggregative Hemorrhagic Escherichia coli O181:H4 Strain Causing Colitis with Hemolytic-Uremic Syndrome." Antibiotics 11, no. 10 (2022): 1416. http://dx.doi.org/10.3390/antibiotics11101416.

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Hybrid diarrheagenic E. coli strains combining genetic markers belonging to different pathotypes have emerged worldwide and have been reported as a public health concern. The most well-known hybrid strain of enteroaggregative hemorrhagic E. coli is E. coli O104:H4 strain, which was an agent of a serious outbreak of acute gastroenteritis and hemolytic uremic syndrome (HUS) in Germany in 2011. A case of intestinal infection with HUS in St. Petersburg (Russian Federation) occurred in July 2018. E. coli strain SCPM-O-B-9427 was obtained from the rectal swab of the patient with HUS. It was determin

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20

Soon, J. M., P. Seaman, and R. N. Baines. "Escherichia coli O104:H4 outbreak from sprouted seeds." International Journal of Hygiene and Environmental Health 216, no. 3 (2013): 346–54. http://dx.doi.org/10.1016/j.ijheh.2012.07.005.

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21

Trung, Nguyen Vinh, Hoang Ngoc Nhung, Juan J. Carrique-Mas, et al. "Colonization of Enteroaggregative Escherichia coli and Shiga toxin-producing Escherichia coli in chickens and humans in southern Vietnam." BMC Microbiology 16, no. 1 (2016): 208. https://doi.org/10.1186/s12866-016-0827-z.

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<strong>Background: </strong>Enteroaggregative (EAEC) and Shiga-toxin producing <i>Escherichia coli</i> (STEC) are a major cause of diarrhea worldwide. <i>E. coli</i> carrying both virulence factors characteristic for EAEC and STEC and producing extended-spectrum beta-lactamase caused severe and protracted disease during an outbreak of <i>E. coli</i> O104:H4 in Europe in 2011. We assessed the opportunities for <i>E. coli</i> carrying the <i>aggR</i> and <i>stx</i> genes to emerge in ‘backyard’ farms in south-east Asia.<strong>Results: </strong>Faecal samples collected from 204 chicken farms; 2

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Molina-López, José, Gerardo Aparicio-Ozores, Rosa María Ribas-Aparicio, et al. "Drug resistance, serotypes, and phylogenetic groups among uropathogenic Escherichia coli including O25-ST131 in Mexico City." Journal of Infection in Developing Countries 5, no. 12 (2011): 840–49. http://dx.doi.org/10.3855/jidc.1703.

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Introduction: The increasing prevalence of uropathogenic Escherichia coli (UPEC) strains resistant to multiple antibiotics complicates the treatment of urinary tract infections (UTIs). This study aimed to analyze the antimicrobial resistance, serotypes, and phylogenetic groups among strains of E. coli isolated from outpatients with UTIs in Mexico City. Methodology: A total of 119 E. coli isolates were recovered from urine samples from outpatients with clinical diagnosis of uncomplicated UTIs from 2004 to 2007. The serotype was assessed by agglutination in microtiter plates; susceptibility to a

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23

Dong, Van Quyen, Thi Lanh Pham, and Kim Oanh Nguyen. "Isolation and characterization of <i>Escherichia coli</i> associated with diarrhea in chickens and ducks in Hai Phong province." Academia Journal of Biology 46, no. 3 (2024): 17–26. http://dx.doi.org/10.15625/2615-9023/20228.

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Colibacillosis is an intestinal tract infection in poultry caused by Escherichia coli. It is one of the leading causes of mortality and results in significant economic losses for the poultry farming sector due to its high incidence rate. Recently, E. coli has been considered a predominant bacterial pathogen that is responsible for diarrhea and bloodstream infections in chickens and ducks in Vietnam. In this study, E. coli strains associated with diarrhea were isolated from chicken and duck feces and intestines collected from poultry farms in Hai Phong province using a Macconkey selective mediu

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24

Ruggenenti, Piero, and Giuseppe Remuzzi. "E. coli O104:H4 German outbreak: a missed opportunity." Nature Reviews Nephrology 8, no. 10 (2012): 558–60. http://dx.doi.org/10.1038/nrneph.2012.194.

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Borgatta, B., N. Kmet-Lunaček, and J. Rello. "E. coli O104:H4 outbreak and haemolytic–uraemic syndrome." Medicina Intensiva 36, no. 8 (2012): 576–83. http://dx.doi.org/10.1016/j.medin.2011.11.022.

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Borgatta, B., N. Kmet-Lunaček, and J. Rello. "E. coli O104:H4 outbreak and haemolytic–uraemic syndrome." Medicina Intensiva (English Edition) 36, no. 8 (2012): 576–83. http://dx.doi.org/10.1016/j.medine.2012.10.009.

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27

Mariani-Kurkdjian, Patricia, Edouard Bingen, Gaëlle Gault, Nathalie Jourdan-Da Silva, and François-Xavier Weill. "Escherichia coli O104:H4 south-west France, June 2011." Lancet Infectious Diseases 11, no. 10 (2011): 732–33. http://dx.doi.org/10.1016/s1473-3099(11)70266-3.

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Kunzendorf, U., and K. Rossen. "Profil einer EHEC-Endemie mit Escherichia coli O104:H4." Der Nephrologe 6, no. 6 (2011): 518–20. http://dx.doi.org/10.1007/s11560-011-0591-x.

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29

Islam, Nazrul, Attila Nagy, Wesley M. Garrett, Dan Shelton, Bret Cooper, and Xiangwu Nou. "Different Cellular Origins and Functions of Extracellular Proteins from Escherichia coli O157:H7 and O104:H4 as Determined by Comparative Proteomic Analysis." Applied and Environmental Microbiology 82, no. 14 (2016): 4371–78. http://dx.doi.org/10.1128/aem.00977-16.

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ABSTRACTExtracellular proteins play important roles in bacterial interactions with the environmental matrices. In this study, we examined the extracellular proteins fromEscherichia coliO157:H7 and O104:H4 by tandem mass spectrometry. We identified 500 and 859 proteins from the growth media ofE. coliO157:H7 and O104:H4, respectively, including 371 proteins common to both strains. Among proteins that were considered specific toE. coliO157:H7 or present at higher relative abundances in O157:H7 medium, most (57 of 65) had secretion signal sequences in their encoding genes. Noticeably, the proteins

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Ortiz, Yaraymi, Alam García-Heredia, Angel Merino-Mascorro, Santos García, Luisa Solís-Soto, and Norma Heredia. "Natural and synthetic antimicrobials reduce adherence of enteroaggregative and enterohemorrhagic Escherichia coli to epithelial cells." PLOS ONE 16, no. 5 (2021): e0251096. http://dx.doi.org/10.1371/journal.pone.0251096.

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Adherence of bacteria to the human intestinal mucosa can facilitate their internalization and the development of pathological processes. Escherichia coli O104:H4 is considered a hybrid strain (enteroaggregative hemorrhagic E. coli [EAHEC]), sharing virulence factors found in enterohemorrhagic (EHEC), and enteroaggregative (EAEC) E. coli pathotypes. The objective of this study was to analyze the effects of natural and synthetic antimicrobials (carvacrol, oregano extract, brazilin, palo de Brasil extract, and rifaximin) on the adherence of EHEC O157:H7, EAEC 042, and EAHEC O104:H4 to HEp-2 cells

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Jackson, Scott A., Michael L. Kotewicz, Isha R. Patel, David W. Lacher, Jayanthi Gangiredla, and Christopher A. Elkins. "Rapid Genomic-Scale Analysis of Escherichia coli O104:H4 by Using High-Resolution Alternative Methods to Next-Generation Sequencing." Applied and Environmental Microbiology 78, no. 5 (2011): 1601–5. http://dx.doi.org/10.1128/aem.07464-11.

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ABSTRACTTwo technologies, involving DNA microarray and optical mapping, were used to quickly assess gene content and genomic architecture of recent emergentEscherichia coliO104:H4 and related strains. In real-time outbreak investigations, these technologies can provide congruent perspectives on strain, serotype, and pathotype relationships. Our data demonstrated clear discrimination between clinically, temporally, and geographically distinct O104:H4 isolates and rapid characterization of strain differences.

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Kim, Myung-Ji, Wim Dejonghe, Murli Manohar, and Jinru Chen. "The Efficacy of Calcium Hypochlorite and Peroxyacetic Acid Treatments in Inactivating Enterohemorrhagic Escherichia coli on Alfalfa Seeds and Sprouts." Microorganisms 13, no. 2 (2025): 306. https://doi.org/10.3390/microorganisms13020306.

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For several decades, recurring outbreaks of human gastrointestinal infections associated with contaminated sprouts have posed an enduring challenge, highlighting the necessity of controlling the etiological agents on contaminated sprout seeds. This study investigated the efficacy of calcium hypochlorite and peroxyacetic acid treatments in inactivating the cells of four enterohemorrhagic Escherichia coli (EHEC) isolates—viz. E. coli O157:H7 K4492, F4546, and H1730, as well as E. coli O104:H4 BAA-2326—on alfalfa seeds and sprouts. The 2–3 log CFU/g of EHEC cells inoculated to sprout seeds became

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Joris, M. A., D. Vanrompay, K. Verstraete, K. De Reu, and L. De Zutter. "Enterohemorrhagic Escherichia coli with particular attention to the German outbreak strain O104:H4." Vlaams Diergeneeskundig Tijdschrift 81, no. 1 (2012): 3–10. http://dx.doi.org/10.21825/vdt.v81i1.18371.

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This review deals with the epidemiology and ecology of enterohemorrhagic Escherichia coli (EHEC), a subset of the verocytotoxigenic Escherichia coli (VTEC), and subsequently discusses its public health concern. Attention is also given to the outbreak strain O104:H4, which has been isolated as causative agent of the second largest outbreak of the hemolytic uremic syndrome worldwide, which started in Germany in May 2011. This outbreak strain is not an EHEC as such but possesses an unusual combination of EHEC and enteroaggregative E. coli (EAggEC) virulence properties.

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Burnett, Vicki L., and David L. Springer. "High-Level Expression of Human Histone H4 in E. Coli." BioTechniques 26, no. 1 (1999): 30–34. http://dx.doi.org/10.2144/99261bm04.

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35

Stockman, J. A. "German Outbreak of Escherichia coli O104:H4 Associated with Sprouts." Yearbook of Pediatrics 2013 (January 2013): 287–89. http://dx.doi.org/10.1016/j.yped.2011.12.017.

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36

Castor, C. "Épidémie d’infections à Escherichia coli O104:H4, Gironde, juin 2011." Revue d'Épidémiologie et de Santé Publique 60 (September 2012): S80. http://dx.doi.org/10.1016/j.respe.2012.06.135.

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Fründt, T., J. Leuffert, A. Gallante, et al. "Untersuchung zum Schadensbild der EHEC 0104:H4 assoziierten Enterokolitis in der koloskopischen Nachsorge." Zeitschrift für Gastroenterologie 56, no. 08 (2018): e222-e222. http://dx.doi.org/10.1055/s-0038-1668707.

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Chen, Shuxiong, Diana H. Quan, Xiaonan T. Wang, et al. "Particulate Mycobacterial Vaccines Induce Protective Immunity against Tuberculosis in Mice." Nanomaterials 11, no. 8 (2021): 2060. http://dx.doi.org/10.3390/nano11082060.

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Currently available vaccines fail to provide consistent protection against tuberculosis (TB). New, improved vaccines are urgently needed for controlling the disease. The mycobacterial antigen fusions H4 (Ag85B-TB10.4) and H28 (Ag85B-TB10.4-Rv2660c) have been shown to be very immunogenic and have been considered as potential candidates for TB vaccine development. However, soluble protein vaccines are often poorly immunogenic, but augmented immune responses can be induced when selected antigens are delivered in particulate form. This study investigated whether the mycobacterial antigen fusions H

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Paddock, Z. D., J. Bai, X. Shi, D. G. Renter, and T. G. Nagaraja. "Detection of Escherichia coli O104 in the Feces of Feedlot Cattle by a Multiplex PCR Assay Designed To Target Major Genetic Traits of the Virulent Hybrid Strain Responsible for the 2011 German Outbreak." Applied and Environmental Microbiology 79, no. 11 (2013): 3522–25. http://dx.doi.org/10.1128/aem.00246-13.

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ABSTRACTA multiplex PCR was designed to detectEscherichia coliO104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregativeE. coli, in cattle feces. A total of 248 fecal samples were tested, and 20.6% were positive for serogroup O104. The O104 isolates did not carry genes characteristic of the virulent hybrid strain.

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DIERCKE, M., M. KIRCHNER, K. CLAUSSEN, et al. "Transmission of shiga toxin-producing Escherichia coli O104:H4 at a family party possibly due to contamination by a food handler, Germany 2011." Epidemiology and Infection 142, no. 1 (2013): 99–106. http://dx.doi.org/10.1017/s0950268813000769.

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SUMMARYWe investigated a cluster of shiga toxin-producing Escherichia coli (STEC) O104:H4 infections after a family party during a large STEC O104:H4 outbreak in Germany. To identify the vehicle we conducted a retrospective cohort study. Stool samples of party guests, and food and environmental samples from the catering company were tested for STEC. We defined cases as party guests with gastrointestinal symptoms and laboratory-confirmed STEC infection. We found 23 cases among 71 guests. By multivariable analysis consumption of salmon [odds ratio (OR) 15, 95% confidence interval (CI) 2·3–97], h

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Mandal, Pintu Kumar. "Synthesis of the pentasaccharide repeating unit of the O-antigen of E. coli O117:K98:H4." Beilstein Journal of Organic Chemistry 10 (November 20, 2014): 2724–28. http://dx.doi.org/10.3762/bjoc.10.287.

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The pentasaccharide repeating unit of the O-antigen of E. coli O117:K98:H4 strain has been synthesized using a combination of sequential glycosylations and [3 + 2] block synthetic strategy from the suitably protected monosaccharide intermediates. Thioglycosides and glycosyl trichloroacetimidate derivatives have been used as glycosyl donors in the glycosylations.

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Zangari, Tonia, Angela R. Melton-Celsa, Aruna Panda, et al. "Virulence of the Shiga Toxin Type 2-Expressing Escherichia coli O104:H4 German Outbreak Isolate in Two Animal Models." Infection and Immunity 81, no. 5 (2013): 1562–74. http://dx.doi.org/10.1128/iai.01310-12.

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ABSTRACTIn May 2011, a large food-borne outbreak was traced to an unusual O104:H4 enteroaggregativeEscherichia coli(EAEC) strain that produced Shiga toxin (Stx) type 2 (Stx2). We developed a mouse model to study the pathogenesis and treatment for this strain and examined the virulence of the isolate for Dutch belted rabbits. O104:H4 strain C227-11 was gavaged into C57BL/6 mice at 109to 1011CFU/animal. The infected animals were then given water with ampicillin (Amp; 5 g/liter)ad libitum. The C227-11-infected, Amp-treated C57BL/6 mice exhibited both morbidity and mortality. Kidneys from mice inf

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Mohamed, Doaa Safwat, Rehab Mahmoud Abd El-Baky, Tim Sandle, Sahar A. Mandour, and Eman Farouk Ahmed. "Antimicrobial Activity of Silver-Treated Bacteria against other Multi-Drug Resistant Pathogens in Their Environment." Antibiotics 9, no. 4 (2020): 181. http://dx.doi.org/10.3390/antibiotics9040181.

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Silver is a potent antimicrobial agent against a variety of microorganisms and once the element has entered the bacterial cell, it accumulates as silver nanoparticles with large surface area causing cell death. At the same time, the bacterial cell becomes a reservoir for silver. This study aims to test the microcidal effect of silver-killed E. coli O104: H4 and its supernatant against fresh viable cells of the same bacterium and some other species, including E. coli O157: H7, Multidrug Resistant (MDR) Pseudomonas aeruginosa and Methicillin Resistant Staphylococcus aureus (MRSA). Silver-killed

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Hartman, Laurie J., Megan L. Heinrich, Ashley M. Zovanyi, et al. "Demonstration of the Pre–Emergency Use Authorization Path Using 3 Minor Groove Binder–Hydrolysis Probe Assays to Detect Escherichia coli O104:H4." Clinical Chemistry 61, no. 11 (2015): 1391–98. http://dx.doi.org/10.1373/clinchem.2015.242750.

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Abstract BACKGROUND The Department of Defense (DoD) and the Food and Drug Administration (FDA) have collaboratively worked on a pre–Emergency Use Authorization (pre-EUA) process for in vitro diagnostic (IVD) devices, using FDA's regulatory flexibilities under the EUA authorities. The pre-EUA process enables FDA review of data in anticipation of a request for an EUA, advancing US government public health emergency preparedness efforts. METHODS The IVD device developed to detect Escherichia coli O104:H4, for which an EUA has not been issued, serves as an example to illustrate that process. Speci

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Kesavan, B., K. V. Srividhya, S. Krishnaswamy, Mugasimangalam Raja, N. Vidya, and A. V. S. K. Krishna Mohan. "Understanding the virulence of the entero-aggregative E. coli O104:H4." International Journal of Bioinformatics Research and Applications 11, no. 3 (2015): 187. http://dx.doi.org/10.1504/ijbra.2015.069185.

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Parolis, Lesley A. S., Haralambos Parolis, and Guy G. S. Dutton. "Structure of the capsular antigen of Escherichia coli O8:K8:H4." Carbohydrate Research 193 (October 1989): 157–63. http://dx.doi.org/10.1016/0008-6215(89)85115-8.

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Sloup, Rudolph E., Roberto J. Cieza, David B. Needle, Robert B. Abramovitch, Alfredo G. Torres, and Christopher M. Waters. "Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104:H4." Biofouling 32, no. 9 (2016): 1131–40. http://dx.doi.org/10.1080/08927014.2016.1230849.

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Johnson, James R., Brian Johnston, Connie Clabots, et al. "Escherichia coli Sequence Type ST131 as an Emerging Fluoroquinolone-Resistant Uropathogen among Renal Transplant Recipients." Antimicrobial Agents and Chemotherapy 54, no. 1 (2009): 546–50. http://dx.doi.org/10.1128/aac.01089-09.

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ABSTRACT Among 40 Escherichia coli urine isolates from renal transplant recipients (Galveston, TX, 2003 to 2005), sequence type ST131 (O25:H4) was highly prevalent (representing 35% of isolates overall and 60% of fluoroquinolone-resistant isolates), virulent appearing, antimicrobial resistant (but extended-spectrum-cephalosporin susceptible), and associated with black race. Pulsotypes were diverse; some were linked to other locales. ST131 emerged significantly during the study period. These findings suggest that E. coli ST131 may constitute an important new multidrug-resistant threat to renal

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Bielaszewska, Martina, Evgeny A. Idelevich, Wenlan Zhang, et al. "Effects of Antibiotics on Shiga Toxin 2 Production and Bacteriophage Induction by Epidemic Escherichia coli O104:H4 Strain." Antimicrobial Agents and Chemotherapy 56, no. 6 (2012): 3277–82. http://dx.doi.org/10.1128/aac.06315-11.

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ABSTRACTThe role of antibiotics in treatment of enterohemorrhagicEscherichia coli(EHEC) infections is controversial because of concerns about triggering hemolytic-uremic syndrome (HUS) by increasing Shiga toxin (Stx) production. During the recent large EHEC O104:H4 outbreak, antibiotic therapy was indicated for some patients. We tested a diverse panel of antibiotics to which the outbreak strain is susceptible to interrogate the effects of subinhibitory antibiotic concentrations on induction ofstx2-harboring bacteriophages,stx2transcription, and Stx2 production in this emerging pathogen. Ciprof

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Bhaumik, Ishani, and Anup Kumar Misra. "Expedient synthesis of a pentasaccharide related to the O-specific polysaccharide of Escherichia coli O117:K98:H4 strain." RSC Adv. 4, no. 106 (2014): 61589–95. http://dx.doi.org/10.1039/c4ra10538a.

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A convenient synthetic strategy has been developed for the synthesis of a pentasaccharide, related to the O-specific polysaccharide ofEscherichia coliO117:K98:H4 strain, using sequential glycosylations of functionalized monosaccharide moieties.

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