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Journal articles on the topic 'E. coli 0157:H7'

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Published: 4 June 2021

Last updated: 17 February 2022

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1

Neill, Marguerite A. "Escherichia coli 0157:H7." Infectious Diseases Newsletter 10, no. 3 (1991): 19–24. http://dx.doi.org/10.1016/0278-2316(91)90046-3.

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2

OKREND, ANITA J. G., BONNIE E. ROSE, and CHARLES P. LATTUADA. "Isolation of Escherichia coli 0157:H7 Using 0157 Specific Antibody Coated Magnetic Beads." Journal of Food Protection 55, no. 3 (1992): 214–17. http://dx.doi.org/10.4315/0362-028x-55.3.214.

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Escherichia coli 0157 specific antibody, coated on magnetic beads, was used to concentrate and remove the E. coli 0157:H7 from mixed cultures and meat samples. The problem of nontarget organism carryover was addressed by adding Protamine to the culture-bead sample, washing the beads three times in saline, and changing the test tubes with each wash. These modifications reduced the nontarget colony counts obtained from uninoculated meat samples. This procedure enabled consistent recovery of E. coli 0157:H7 from inoculated meat samples. The percentage of E. coli 0157:H7 cells captured, compared t

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3

Roberts, James R. "Escherichia coli 0157:H7 Infections." Emergency Medicine News 26, no. 13 (2004): 20–22. http://dx.doi.org/10.1097/00132981-200401000-00020.

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4

Rowe, PaulM. "Eradicating E coli 0157:H7." Lancet 345, no. 8942 (1995): 117. http://dx.doi.org/10.1016/s0140-6736(95)90075-6.

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5

Hammack, Thomas S., Peter Feng, R. Miguel Amaguaña, Geraldine June, Patricia S. Sherrod, and Wallace H. Andrews. "Comparison of Sorbitol MacConkey and Hemorrhagic Coli Agars for Recovery of Escherichia coli 0157:H7 from Brie, Ice Cream, and Whole Milk." Journal of AOAC INTERNATIONAL 80, no. 2 (1997): 335–40. http://dx.doi.org/10.1093/jaoac/80.2.335.

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Abstract The relative efficacies of hemorrhagic coli (HC) agar and several formulations of sorbitol Mac-Conkey (SorMac) agar, with and without 0.1 % (w/v) 4-methyllumbelliferyl-ß-D-glucuronide (MUG), in recovering unstressed and heat-stressed Escherichia coli 0157:H7 from Brie cheese, ice cream, and whole milk were determined. Recovery of unstressed E. coli 0157:H7 was determined quantitatively by spread-plating diluted samples onto different agars and performing plate counts. Recovery of stressed E. coli 0157:H7 was determined qualitatively by enriching samples in modified trypticase soy brot

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6

Mcgowan, KarinL, Ellen Wickersham, and NancyA Strockbine. "ESCHERICHIA COLI 0157:H7 FROM WATER." Lancet 333, no. 8644 (1989): 967–68. http://dx.doi.org/10.1016/s0140-6736(89)92559-2.

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7

Yannelli, Barbara, Philip Domenico, Burke A. Cunha, and Sayed M. H. Quadri. "Nosocomial Escherichia coli 0157:H7 diarrhea." American Journal of Infection Control 18, no. 5 (1990): 341–42. http://dx.doi.org/10.1016/0196-6553(90)90236-l.

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8

Griffin, Patricia M., Linda C. Olmstead, and Robert E. Petras. "Escherichia coli 0157:H7-associated colitis." Gastroenterology 99, no. 1 (1990): 142–49. http://dx.doi.org/10.1016/0016-5085(90)91241-w.

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9

RONNER, AMY B., and DEAN O. CLIVER. "Isolation and Characterization of a Coliphage Specific for Escherichia coli 0157:H7." Journal of Food Protection 53, no. 11 (1990): 944–47. http://dx.doi.org/10.4315/0362-028x-53.11.944.

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Enterohemorrhagic Escherichia coli serogroup 0157:H7 is harbored by cattle and causes bloody diarrhea and hemolytic uremic syndrome in persons who consume raw milk and under-cooked beef. Samples of manure from Wisconsin dairy farms were tested for the presence of E. coli 0157:H7 as well as for bacteriophages (coliphages) specific for this microorganism. No E. coli 0157:H7 bacteria were isolated from any of the 21 manure samples taken from 12 farms. Nineteen of 20 samples yielded “nonspecific” coliphages that produced plaques both on 0157:H7 and on other E. coli. Only one sample yielded a colip

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10

OKREND, ANITA J. G., BONNIE E. ROSE та CHARLES P. LATTUADA. "Use of 5-Bromo-4-Chloro-3-lndoxyl-β-D-Glucuronide in MacConkey Sorbitol Agar to Aid in the Isolation of Escherichia coli 0157:H7 from Ground Beef". Journal of Food Protection 53, № 11 (1990): 941–43. http://dx.doi.org/10.4315/0362-028x-53.11.941.

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The addition of 5-bromo-4-chloro-3-indoxyl-β-D-glucuronide (BCIG) at the 0.1 g/L level, to MacConkey sorbitol agar (MSA) plates aided in the isolation of Escherichia coli 0157:H7 from raw ground beef samples by differentiating β-glucuronidase positive from β-glucuronidase negative colonies. E. coli 0157:H7 colonies, being sorbitol negative, β-glucuronidase negative, remained white, while sorbitol negative, β-glucuronidase positive colonies turned green to blue. Addition of BCIG to the MSA agar reduced the number of false suspect colonies picked from the primary plating medium by 36% when compa

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11

CIMOLAI, NEVIO, JOHN D. ANDERSON, and BRENDA J. MORRISON. "Antibiotics for Escherickia coli 0157: H7 enteritis?" Journal of Antimicrobial Chemotherapy 23, no. 5 (1989): 807–8. http://dx.doi.org/10.1093/jac/23.5.807.

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12

Lee, J. M., B. Crowley, P. A. Cahill, et al. "Escherichia coli 0157: H7 — Two case reports." Irish Journal of Medical Science 166, no. 1 (1997): 20–22. http://dx.doi.org/10.1007/bf02939770.

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13

Cahoon, Frederic E., and J. Stephen Thompson. "Frequency of Escherichia coli 0157:H7 isolation from stool specimens." Canadian Journal of Microbiology 33, no. 10 (1987): 914–15. http://dx.doi.org/10.1139/m87-158.

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During a 6-month period, 7252 faeces specimens were examined for Escherichia coli serotype0157:H7. Forty-nine specimens (0.7%) from 19 patients yielded this organism. Escherichia coli 0157:H7 was the third most frequently isolated bacterial pathogen, following Campylobacter jejuni and (or) Salmonella sp. Although regional variation between laboratories determined whether Campylobacter jejuni or Salmonella was the primary bacterial pathogen isolated, E. coli 0157:H7 was consistently isolated more frequently than either Shigella or Yersinia enterocolitica.

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14

Entis, Phyllis, D. Bryant, J. Bryant, et al. "Direct 24-Hour Presumptive Enumeration of Escherichia coli 0157:H7 in Foods Using Hydrophobic Grid Membrane Filter Followed by Serological Confirmation: Collaborative Study." Journal of AOAC INTERNATIONAL 81, no. 2 (1998): 403–18. http://dx.doi.org/10.1093/jaoac/81.2.403.

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abstract Fifteen laboratories took part in a collaborative study to validate a method for enumerating Escherichia coli 0157:H7. The method is based on use of a hydrophobic grid membrane filter and consists of 24 h presumptive enumeration on SD-39 Agar and serological confirmation to yield a confirmed E. coli 0157:H7 count. Six food products were analyzed: pasteurized apple cider, pasteurized 2% milk, cottage cheese, cooked ground pork, raw ground beef, and frozen whole egg. The test method produced significantly higher confirmed count results than did the reference method for milk, pork, and b

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15

Cohen, Alice E., and Khalil F. Kerdahi. "Evaluation of a Rapid and Automated Enzyme-Linked Fluorescent Immunoassay for Detecting Escherichia coli Serogroup 0157 in Cheese." Journal of AOAC INTERNATIONAL 79, no. 4 (1996): 858–60. http://dx.doi.org/10.1093/jaoac/79.4.858.

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Abstract The Vitek Immunodiagnostic Assay System (VIDAS) for Escherichia coli 0157, a rapid and fully automated test, was evaluated for use in detecting the foodborne pathogen E. coli 0157:H7 in soft, semisoft, and hard cheeses. Sixty-five cheese samples were artificially contaminated at low (2-4 colonyforming units [cfu]/25 g) and high (7-10 cfu/25 g) levels with one of 2 strains of enterohemorrhagic E. coli 0157:H7. Contamination at high levels was detected in all cheeses by VIDAS, whereas in 5 cheeses (7.7%) inoculated at low levels, contamination was not detected. In 15 additional cheeses

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16

GOVARIS (Α. ΓΚΟΒΑΡΗΣ), A., P. KOIDIS (Π. ΚΟΪΔΗΣ), and K. PAPATHEODOROU (Κ. ΠΑΠΑΘΕΟΔΩΡΟΥ). "Survival of Escherichia coli 0157:H7 in Feta cheese during storage." Journal of the Hellenic Veterinary Medical Society 53, no. 1 (2018): 24. http://dx.doi.org/10.12681/jhvms.15356.

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The survival of E. co/i0157:H7 in Feta cheese and the brine used after 2 months ripening of cheese, was studied during storage at 4°C and 12°C. An inoculum of 5.3 log10CFU/ml of E. coli Ol57:H7 was added in brine, for the contamination with the pathogen of Feta cheese. E. coli Ol 57:H7 was detectable in brine by direct plating on SMAC up to 40th day and 32th day during its storage at 4°C and 12°C respectively, while by enrichment and following plating on SMAC up to 44th day (4°C) and 36th day (12°C). The E. coli 0157:H7 was detectable in Feta by direct plating on SMAC up to 44th day (4°C) and

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17

GRANSDEN, W. R. "Verocytotoxin-Producing Escherichia coli 0157:H7 and Diarrhea." Annals of Internal Medicine 103, no. 1 (1985): 160. http://dx.doi.org/10.7326/0003-4819-103-1-160.

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18

Borczyk, A. A., H. Lior, and S. Thompson. "Sorbitol-negative Escherichia coli 0157 other than H7." Journal of Infection 18, no. 2 (1989): 198–99. http://dx.doi.org/10.1016/s0163-4453(89)91486-2.

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19

Beutin, L., G. Knollmann-Schanbacher, W. Rietschel, and H. Seeger. "Animal reservoirs of Escherichia coli 0157:I[H7]." Veterinary Record 139, no. 3 (1996): 70–71. http://dx.doi.org/10.1136/vr.139.3.70.

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20

MacDonald, Kristine L. "Escherichia coli 0157:H7, an Emerging Gastrointestinal Pathogen." JAMA 259, no. 24 (1988): 3567. http://dx.doi.org/10.1001/jama.1988.03720240029030.

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21

Morandi, Eugenio, Chiara Grassi, Paola Cellerino, Paolo Previde Massara, Fabio Corsi, and Emilio Trabucchi. "Verocytotoxin-Producing Escherichia coli EH 0157:H7 Colitis." Journal of Clinical Gastroenterology 36, no. 1 (2003): 44–46. http://dx.doi.org/10.1097/00004836-200301000-00014.

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22

TORTORELLO, MARY L., and STEVEN M. GENDEL. "Fluorescent Antibodies Applied to Direct Epifluorescent Filter Technique for Microscopic Enumeration of Escherichia coli 0157:H7 in Milk and Juice." Journal of Food Protection 56, no. 8 (1993): 672–77. http://dx.doi.org/10.4315/0362-028x-56.8.672.

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In a modification of the direct epifluorescent filter technique (DEFT), direct fluorescent antibody staining was used for the rapid (&lt;1 h), specific enumeration of foodbome Escherichia coli 0157:H7 by epifluorescence microscopy. Cell counts obtained by this method (Ab-DEFT) correlated well with DEFT counts obtained by acridine orange staining and with viable plate counts ranging from 108 to 101 cells per ml for pure cultures in buffer. Ab-DEFT also was effective for counting E. coli 0157:H7 cells inoculated into milk and juice; the sensitivity limit was about 103 for milk. The highly sp

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23

Mosharraf, Fahareen Binta, Chowdhury Rafiqul Ahsan, Jamalun Nessa, and Mahmuda Yasmin. "Exploring the best possible route of infection of Verotxin producing Escherichia coli in development of experimental gastroenteritis among streptomycin treated murine model." Journal of Bangladesh Academy of Sciences 39, no. 1 (2015): 53–58. http://dx.doi.org/10.3329/jbas.v39i1.23658.

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The prevalence of Escherichia coli O157:H7 induced bacteremia generate a critical problem in modern medical therapy for bacterial infections. This study sought to find out the best possible route and dose of E. coli 0157:H7 infection in experimental murine model by periodic stool and blood culture count of relevant bacterial strains. Streptomycin treated mouse model were used for investigating the clinical manifestation exerted by stx1A and stx2A positive E. coli O157:H7 with increasing doses applied through three alternative routes (oral, intramuscular and intraperitoneal). The highest titer

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24

Borczyk, A. A., M. A. Karmali, H. Lior, and L. M. C. Duncan. "BOVINE RESERVOIR FOR VEROTOXIN-PRODUCING ESCHERICHIA COLI 0157:H7." Lancet 329, no. 8524 (1987): 98. http://dx.doi.org/10.1016/s0140-6736(87)91928-3.

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25

Perna, Nicole T., Guy Plunkett, Valerie Burland, et al. "erratum Genome sequence of enterohaemorrhagic Escherichia coli 0157:H7." Nature 410, no. 6825 (2001): 240. http://dx.doi.org/10.1038/35065664.

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26

Feldsine, Philip T., Robin L. Forgey, Maria T. Falbo-Nelson, and Sharon L. Brunelle. "Assurance Escherichia coli 0157:H7: A Comparative Validation Study." Journal of AOAC INTERNATIONAL 80, no. 1 (1997): 37–42. http://dx.doi.org/10.1093/jaoac/80.1.37.

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Abstract A wide variety of food products, with emphasis on raw meat products, were analyzed simultaneously by 3 methods: the Assurance Escherichia coli 0157:H7 (EHEC) enzyme immunosorbent assay (EIA), the EHEC visual immunoprecipitate assay (VIP), and a modified Bacteriological Analytical Manual (BAM) culture method. This paper reports results of a comparative study of the Assurance and modified BAM methods. In this comparative study, 1050 samples and controls gave false-negative rates of 1.0 and 0%, respectively, for the Assurance EIA and the modified BAM culture methods. The overall agreemen

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27

Ostroff, Stephen M. "Infections With Escherichia coli 0157:H7 in Washington State." JAMA 262, no. 3 (1989): 355. http://dx.doi.org/10.1001/jama.1989.03430030043031.

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28

NEILL, MARGUERITE A. "E. COLI 0157:H7 -CURRENT CONCEPTS AND FUTURE PROSPECTS." Journal of Food Safety 10, no. 2 (2007): 99–106. http://dx.doi.org/10.1111/j.1745-4565.1989.tb00012.x.

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29

Cieslak, PaulR, TimothyJ Barrett, PatriciaM Griffin, et al. "Escherichia coli 0157:H7 infection from a manured garden." Lancet 342, no. 8867 (1993): 367. http://dx.doi.org/10.1016/0140-6736(93)91509-k.

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30

Looper, M. L., T. S. Edrington, T. R. Callaway, et al. "Influence of toxic enophyte-infected tall fescue on the prevalence of E. coli 0157:H7 from ruminants, a review." NZGA: Research and Practice Series 13 (January 1, 2007): 87–89. http://dx.doi.org/10.33584/rps.13.2006.3092.

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Ruminants consuming toxic endophyte-infected (E+) tall fescue are exposed to ergot alkaloids that usually result in a malady of problems identified as fescue toxicosis. Grazing studies investigating the effects of E+ tall fescue on the prevalence of E. coli O157:H7 from naturally-infected cattle have not been consistent. Further, in vitro studies with selected ergot alkaloids did not affect growth of E. coli O157:H7. Dietary stressors usually associated with E+ tall fescue consumption did result in increased faecal shedding of E. coli O157:H7 from sheep experimentally-inoculated. Animal specie

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31

GOVARIS (Α. ΓΚΟΒΑΡΗΣ), A., D. K. PAPAGEORGIOU (Δ.Κ. ΠΑΠΑΓΕΩΡΓΙΟΥ), and K. PAPATHEODOROU (Κ. ΠΑΠΑΘΕΟΔΩΡΟΥ). "Survival of Escherichia coli 0157:H7 in cultured butter during storage." Journal of the Hellenic Veterinary Medical Society 53, no. 2 (2018): 147. http://dx.doi.org/10.12681/jhvms.15371.

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The survival of E. coli 0157:H7 was examined in 3 types of butter: unsalted, salted with 0.46% and 0.93% salt. The butter samples were inoculated with a low (c.a. 3.14 log CFU/g) or high concentration (c.a. 4.80 log CFU/g) inoculum of the pathogen and were stored at 4°C and 12°C. The contaminated butter samples were stored for 2 months at 4°C and up to visible spoilage (20-26 days) at 12° C. By the end of the storage at 4°C, the tests with the high inoculum of the pathogen revealed that populations of E. coli 0157:H7 were decreased by 2.26 log CFU/g in the unsalted and in 0.46% salted types of

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32

Mulvey, George, David J. Rafter, and Glen D. Armstrong. "Potential for using antibiotics combined with a Shiga toxin-absorbing agent for treating 0157:H7 Escherichia coli infections." Canadian Journal of Chemistry 80, no. 8 (2002): 871–74. http://dx.doi.org/10.1139/v02-028.

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Antibiotics are not recommended for treating O157:H7 Escherichia coli infections because they may promote Shiga toxin (Stx) release from these bacteria. This could increase the risk of Stx-mediated complications in patients suffering from such infections. Here, we observed increased cell-free Stx in E. coli O157:H7 cultures exposed to sub-inhibitory concentrations of several antibiotics. Synsorb-Pk, an agent with a high affinity for Stx, absorbed Stx activity from the antibiotic-treated cultures. These data suggest certain antibiotics, given in combination with an orally administered Stx-bindi

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33

Feldsine, Philip T., Maria T. Falbo-Nelson, Sharon L. Brunelle, et al. "Assurance Enzyme Immunoassay for Detection of Enterohemorrhagic Escherichia coli 0157:H7 in Selected Foods: Collaborative Study." Journal of AOAC INTERNATIONAL 80, no. 3 (1997): 530–44. http://dx.doi.org/10.1093/jaoac/80.3.530.

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Abstract Five foods types were analyzed by the Assurance EHEC (Escherichia coli 0157:H7) enzyme immunoassay (EIA) and by the Bacteriological Analytical Manual (BAM) culture method. Each sample of each food type at each inoculation level was simultaneously analyzed by both methods. A total of 21 laboratories representing state and federal government agencies and private industry in the United States and Canada participated. Samples were inoculated with E. coli 0157:H7, except for one lot of poultry that was naturally contaminated. A total of 1304 samples and controls were analyzed and confirmed

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34

Robson, W. Lane M. "The Hemolytic-Uremic Syndrome and Escherichia coli 0157: H7 Infection." Annals of Internal Medicine 119, no. 3 (1993): 249. http://dx.doi.org/10.7326/0003-4819-119-3-199308010-00025.

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35

Swerdlow, David L. "The Hemolytic-Uremic Syndrome and Escherichia coli 0157: H7 Infection." Annals of Internal Medicine 119, no. 3 (1993): 249. http://dx.doi.org/10.7326/0003-4819-119-3-199308010-00026.

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36

Ørskov, F., I. Ørskov, and J. A. Villar. "CATTLE AS RESERVOIR OF VEROTOXIN-PRODUCING ESCHERICHIA COLI 0157:H7." Lancet 330, no. 8553 (1987): 276. http://dx.doi.org/10.1016/s0140-6736(87)90860-9.

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37

Mead, Paul S. "Risk Factors for Sporadic Infection With Escherichia coli 0157:H7." Archives of Internal Medicine 157, no. 2 (1997): 204. http://dx.doi.org/10.1001/archinte.1997.00440230076009.

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38

Rice, D. H., D. D. Hancock, and T. E. Besser. "Faecal culture of wild animals for Escherichia coli 0157:H7." Veterinary Record 152, no. 3 (2003): 82–83. http://dx.doi.org/10.1136/vr.152.3.82.

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39

Shortsleeve, Michael J., Mary E. Wilson, Mark Finklestein, and Richard C. Gardner. "Radiologic findings in hemorrhagic colitis due toEscherichia coli 0157:H7." Gastrointestinal Radiology 14, no. 1 (1989): 341–44. http://dx.doi.org/10.1007/bf01889233.

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40

SAMOURIS (Γ. ΣΑΜΟΥΡΗΣ), G., A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ), G. VAFEAS (Γ. ΒΑΦΕΑΣ), and S. BELIBASAKI (Σ. ΜΠΕΛΙΜΠΑΣΑΚΗ). "Survival of pathogens in "Graviera Kritis" cheese made with raw and pasteurized milk." Journal of the Hellenic Veterinary Medical Society 62, no. 3 (2017): 205. http://dx.doi.org/10.12681/jhvms.14850.

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A field trial was carried out to investigate the hygienic status of Graviera 'Kritis' cheese made with raw and pasteurized milk. Graviera was manufactured in a small plant in Crete under traditional manufacturing conditions. Forty-two samples were examined during the manufacturing process for Colifoms, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Salmonella spp., Escherìchia coli 0157:H7 and VRE (Vancomycin Resistant Enterococci). All samples were negative for Salmonella spp., E. coli 0157:H7 and VRE. Populations of Colifoms and E. coli before cooking of the curd were 3 3.5

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41

Feldsine, Philip T., Robin L. Forgey, Maria T. Falbo-Nelson, and Sharon L. Brunelle. "Escherichia coli 0157:H7 Visual Immunoprecipitate Assay: A Comparative Validation Study." Journal of AOAC INTERNATIONAL 80, no. 1 (1997): 37–42. http://dx.doi.org/10.1093/jaoac/80.1.43.

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Abstract A wide variety of food products, with emphasis on raw meat products, were analyzed simultaneously by 3 methods: the Assurance Escherichia coli 0157:H7 (EHEC) enzyme immunosorbent assay (EIA), the EHEC visual immunoprecipitate assay (VIP), and a modified Bacteriological Analytical Manual (BAM) culture method. This paper reports results of a comparative study of the Assurance and modified BAM methods. In this comparative study, 1050 samples and controls gave false-negative rates of 1.0 and 0%, respectively, for the Assurance EIA and the modified BAM culture methods. The overall agreemen

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42

Smith, SI, OO Aboaba, P. Odeigha, et al. "Plasmid profile of Escherichia coli 0157:H7 from apparently healthy animals." African Journal of Biotechnology 2, no. 9 (2003): 322–24. http://dx.doi.org/10.5897/ajb2003.000-1066.

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43

., O. O. Aboaba, S. I. Smith ., and F. O. Olude . "Antibacterial Effect of Edible Plant Extract on Escherichia coli 0157:H7." Pakistan Journal of Nutrition 5, no. 4 (2006): 325–27. http://dx.doi.org/10.3923/pjn.2006.325.327.

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44

Grzadkowska, D., and M. W. Griffiths. "Cryotolerance of Escherichia coli 0157:H7 in Laboratory Media and Food." Journal of Food Science 66, no. 8 (2008): 1169–73. http://dx.doi.org/10.1111/j.1365-2621.2001.tb16100.x.

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45

Woodward, M. J., D. Gavier-Widen, I. M. McLaren, C. Wray, M. Sozmen, and G. R. Pearson. "Infection of gnotobiotic calves with Escherichia coli 0157:H7 strain A84." Veterinary Record 144, no. 17 (1999): 466–70. http://dx.doi.org/10.1136/vr.144.17.466.

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46

Eriksson, E., E. Nerbrink, E. Borch, A. Aspan, and A. Gunnarsson. "Verocytotoxin-producing Escherichia coli 0157:H7 in the Swedish pig population." Veterinary Record 152, no. 23 (2003): 712–17. http://dx.doi.org/10.1136/vr.152.23.712.

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47

Brasitus, T. A. "Sporadic cases of hemorrhagic colitis caused by Escherichia coli 0157:H7." Gastroenterology 89, no. 4 (1985): 919. http://dx.doi.org/10.1016/0016-5085(85)90593-1.

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48

Mohammed, Zuhair A. "Isolation and Identification of Escherichia coli O157:H7 from locally minced meat and imported minced and chicken meat." Iraqi Journal of Veterinary Medicine 32, no. 1 (2008): 100–113. http://dx.doi.org/10.30539/iraqijvm.v32i1.771.

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A total of 143 samples of meat were examined in this study, theserepresents 67 samples of locally minced meat, 31 samples of importedminced meat and 45 samples of imported chicken meat. The samples werecollected from different areas of Baghdad city during the period fromJanuary till May 2006.Detection, isolation an enumeration of E.coli 0157:H7 were carried out.The result revealed that there was a significant difference (P < 0.05) in theisolation percentage and the microbial counts of E.coli 0157:H7 whencomparing the three types of meat. The highest prevalence were found in thelocally mince

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KANDA, TAKASHI, KANJI SHIOZAWA, FUMIKAZU SATO, KEISUKE AOKI, and TOKUHIRO NISHINA. "Isolation of Escherichia coli 0157: H7 from Feces of Cattle in Japan." Journal of the Japan Veterinary Medical Association 45, no. 1 (1992): 45–47. http://dx.doi.org/10.12935/jvma1951.45.45.

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Baines, Danica, Luke Masson, and Tim McAllister. "A rapid, sensitive method for testing the activity of Escherichia coli 0157:H7 secreted cytotoxins against epithelial cells from the jejunum and descending colon of cattle." Canadian Journal of Animal Science 88, no. 1 (2008): 51–55. http://dx.doi.org/10.4141/cjas07040.

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Abstract:

Current methods for assessing Escherichia coli O157:H7 secreted cytotoxin activity are based on exposing human cell lines for 12 to 72 h and monitoring changes in cell morphology, adherence or enzyme release. These methods, although sensitive, use non-ruminant cell lines that may not represent cattle responses to E. coli O157:H7 secreted cytotoxins. The modified lawn assay used in this study was found to be a simple, fast and sensitive method for assessing cattle epithelial cell susceptibility to E. coli O157:H 7 secreted cytotoxins within 4 to 6 h. Key words: Escherichia coli O157:H7, cattle,

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