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Relevant bibliographies by topics / E.coli O157

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Academic literature on the topic 'E.coli O157'

Author: Grafiati

Published: 4 June 2021

Last updated: 16 June 2025

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Journal articles on the topic "E.coli O157"

1

HUSSEIN, HUSSEIN S., LAURIE M. BOLLINGER, and MARK R. HALL. "Growth and Enrichment Medium for Detection and Isolation of Shiga Toxin–Producing Escherichia coli in Cattle Feces." Journal of Food Protection 71, no. 5 (2008): 927–33. http://dx.doi.org/10.4315/0362-028x-71.5.927.

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Detection methods of Shiga toxin–producing Escherichia coli (STEC) in cattle feces varied in using enrichment media containing different antibiotic combinations. To examine efficacy of a new detection method for STEC, three O157:H7 (ATCC 43889, 43890, and 43895) and 41 non-O157:H7 (members of the O1, O15, O26, O86, O103, O111, O125, O127, O128, O136, O146, O153, O158, O165, O166, and O169 serogroups) isolates were tested. These isolates were grown in tryptic soy broth for 6 h, and their concentrations were determined before inoculation of tubes containing 1 g of cattle feces (sterile [experiment 1; evaluating growth] and fresh [experiment 2; evaluating enrichment]) to simulate the high and low levels of STEC shedding by cattle (105 versus 102 CFU/g feces, respectively). Eight STEC isolates (the three O157:H7 and five non-O157:H7 selected at random) were tested at a very low level (10 CFU/g feces). The feces were incubated in 50 ml of brain heart infusion broth containing potassium tellurite, novobiocin, and vancomycin (2.5, 20, and 40 mg/liter, respectively) and cefixime (50 μg/liter) at 37°C for 12 h and tested for STEC (VTEC [verotoxin-producing E. coli]–Screen assay [agglutination immunoassay]). Potential STEC isolates were recovered, characterized biochemically, serotyped, and tested for toxin production using Vero (African green monkey kidney) cell toxicity assay and agglutination immunoassay. In both experiments, all the STEC isolates used for fecal inoculation were recovered at the concentrations tested. Our medium supported growth of and enrichment for a wide range of STEC isolates.

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Son, W. G., T. A. Graham та V. P. J. Gannon. "Immunological Characterization of Escherichia coli O157:H7 Intimin γ1". Clinical and Vaccine Immunology 9, № 1 (2002): 46–53. http://dx.doi.org/10.1128/cdli.9.1.46-53.2002.

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ABSTRACT Portions of the intimin genes of Escherichia coli O157:H7 strain E319 and of the enteropathogenic E. coli O127:H6 strain E2348/69 were amplified by PCR and cloned into pET-28a(+) expression vectors. The entire 934 amino acids (aa) of E. coli O157:H7 intimin, the C-terminal 306 aa of E. coli O157:H7 intimin, and the C-terminal 311 aa of E. coli O127:H6 intimin were expressed as proteins fused with a six-histidine residue tag (six-His tag) in pET-28a(+). Rabbit antisera raised against the six-His tag-full-length E. coli O157:H7 intimin protein fusion cross-reacted in slot and Western blots with outer membrane protein preparations from the majority of enterohemorrhagic and enteropathogenic E. coli serotypes which have the intimin gene. The E. coli strains tested included isolates from humans and animals which produce intimin typesα (O serogroups 86, 127, and 142), β1 (O serogroups 5, 26, 46, 69, 111, 126, and 128), γ1 (O serogroups 55, 145, and 157), γ2 (O serogroups 111 and 103), and ε (O serogroup 103) and a nontypeable intimin (O serogroup 80), results based on intimin type-specific PCR assays. Rabbit antisera raised against the E. coli O157:H7 C-terminal fusion protein were much more intimin type-specific than those raised against the full-length intimin fusion protein, but some cross-reaction with other intimin types was also observed for these antisera. In contrast, the monoclonal antibody Intγ1.C11, raised against the C-terminal E. coli O157 intimin, reacted only with preparations from intimin γ1-producing E. coli strains such as E. coli O157:H7.

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Gamage, Shantini D., Colleen M. McGannon, and Alison A. Weiss. "Escherichia coli Serogroup O107/O117 Lipopolysaccharide Binds and Neutralizes Shiga Toxin 2." Journal of Bacteriology 186, no. 16 (2004): 5506–12. http://dx.doi.org/10.1128/jb.186.16.5506-5512.2004.

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ABSTRACT The AB5 toxin Shiga toxin 2 (Stx2) has been implicated as a major virulence factor of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains in the progression of intestinal disease to more severe systemic complications. Here, we demonstrate that supernatant from a normal E. coli isolate, FI-29, neutralizes the effect of Stx2, but not the related Stx1, on Vero cells. Biochemical characterization of the neutralizing activity identified the lipopolysaccharide (LPS) of FI-29, a serogroup O107/O117 strain, as the toxin-neutralizing component. LPSs from FI-29 as well as from type strains E. coli O107 and E. coli O117 were able bind Stx2 but not Stx1, indicating that the mechanism of toxin neutralization may involve inhibition of the interaction between Stx2 and the Gb3 receptor on Vero cells.

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NARANG, NEELAM, PINA M. FRATAMICO, GLENN TILLMAN, KITTY PUPEDIS, and WILLIAM C. CRAY. "Performance Comparison of a fliCh7 Real-Time PCR Assay with an H7 Latex Agglutination Test for Confirmation of the H Type of Escherichia coli O157:H7†." Journal of Food Protection 72, no. 10 (2009): 2195–97. http://dx.doi.org/10.4315/0362-028x-72.10.2195.

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Escherichia coli O157:H7 is a foodborne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. Positive identification of E. coli O157:H7 is made using biochemical tests and specific antisera or latex agglutination reagents for the O157 and H7 antigens. However, under certain conditions, some E. coli O157:H7 isolates can appear to be nonreactive with H7 antisera and may require multiple passages on motility medium to restore H7 antigenicity. In this study, we compared the performance of a real-time PCR test with that of a method using latex agglutination reagents to detect the presence of the fliCh7 gene or the H7 antigen, respectively, in E. coli O157:H7 isolates. One hundred twenty-six E. coli strains were tested including reference strains and strains isolated from meat. Lyophilized E. coli O157:H7 isolates were rehydrated and were plated on sheep blood agar without passage on motility medium. All strains were analyzed in parallel by a real-time PCR test targeting the fliCh7 gene and by a latex agglutination test that detects the H7 antigen. The real-time PCR assay showed 100% agreement with the H7 status reported for reference strains and E. coli O157:H7 meat isolates. The latex agglutination test results agreed with the H7 status reported for the E. coli O157:H7 reference strains and non-O157:H7 strains, except for one, E. coli O117:H7; however, 42% (42 of 100) of the E. coli O157:H7 meat isolates tested negative for the H7 antigen by latex agglutination. The real-time fliCh7 PCR test can be used to confirm E. coli O157:H7 strains that are not expressing the immunoreactive H7 antigen.

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KANG, DONG-HYUN, and DANIEL Y. C. FUNG. "Development of a Medium for Differentiation between Escherichia coli and Escherichia coli O157:H7." Journal of Food Protection 62, no. 4 (1999): 313–17. http://dx.doi.org/10.4315/0362-028x-62.4.313.

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A new medium (Escherichia coli O157:H7 medium: EOH) was developed for differentiation between E. coli and E. coli O157:H7. The EOH medium was compared with sorbitol MacConkey agar (SMAC), which is the most popular medium to enumerate E. coli O157:H7. Several combinations of 35 dyes were evaluated to develop the new medium. Indigo carmine (0.03 g/liter) and phenol red (0.036 g/liter) were found as the best combination for differentiation between E. coli O157:H7 and E. coli and added to the basal agar medium (SMAC medium excluding neutral red and crystal violet) for EOH medium. On the dark blue EOH medium, E. coli produced a yellow color with clear zone, whereas E. coli O157:H7 produced a red color without clear zone. For differentiation between E. coli and E. coli O157:H7, EOH has much better potential than SMAC. Furthermore, the red color produced by normal E. coli in SMAC may mask the light gray color produced by E. coli O157: H7, whereas the yellow color with clear zone did not mask the red color without clear zone in the EOH medium. The recovery numbers of E. coli O157:H7 from inoculated ground beef, pork, and turkey were not significantly different between SMAC and EOH media (P &gt; 0.05). The recovery rates of heat- and cold-injured E. coli O157:H7 also were not significantly different (P &gt; 0.05).

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LEENANON, B., and M. A. DRAKE. "Acid Stress, Starvation, and Cold Stress Affect Poststress Behavior of Escherichia coli O157:H7 and Nonpathogenic Escherichia coli†." Journal of Food Protection 64, no. 7 (2001): 970–74. http://dx.doi.org/10.4315/0362-028x-64.7.970.

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The effects of acid shock, acid adaptation, starvation, and cold stress of Escherichia coli O157:H7 (ATCC 43895), an rpo S mutant (FRIK 816-3), and nonpathogenic E. coli (ATCC 25922) on poststress heat resistance and freeze–thaw resistance were investigated. Following stress, heat tolerance at 56°C and freeze–thaw resistance at −20 to 21°C were determined. Heat and freeze–thaw resistance of E. coli O157:H7 and nonpathogenic E. coli was enhanced after acid adaptation and starvation. Following cold stress, heat resistance of E. coli O157:H7 and nonpathogenic E. coli was decreased, while freeze–thaw resistance was increased. Heat and freeze–thaw resistance of the rpoS mutant was enhanced only after acid adaptation. Increased or decreased tolerance of acid-adapted, starved, or cold-stressed E. coli O157:H7 cells to heat or freeze–thaw processes should be considered when processing minimally processed or extended shelf-life foods.

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MEKATA, HIROHISA, ATSUSHI IGUCHI, KIMIKO KAWANO, YUMI KIRINO, IKUO KOBAYASHI, and NAOAKI MISAWA. "Identification of O Serotypes, Genotypes, and Virulotypes of Shiga Toxin–Producing Escherichia coli Isolates, Including Non-O157 from Beef Cattle in Japan." Journal of Food Protection 77, no. 8 (2014): 1269–74. http://dx.doi.org/10.4315/0362-028x.jfp-13-506.

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Bovines are recognized as an important reservoir of Shiga toxin–producing Escherichia coli (STEC). Although STEC strains are significant foodborne pathogens, not all of the STEC held by cattle are pathogenic, and which type of STEC that will become epidemic in humans is unpredictable. Information about the prevalence of serotype and virulence gene distribution in beef cattle is insufficient to develop monitoring and controlling activities for a food safety and security program. Thus, this study investigated the prevalence of O157 and non-O157 STEC in Japanese beef cattle and characterized the isolates by the type of O antigen and several virulence markers to help predict the pathogenicity. In this study, 64.2%(176 of 274) of enrichment cultures of fecal samples collected from an abattoir and farms were stx1 and/or stx2 positive by PCR. STEC strains were isolated from 22.1% (39 of 176) of the positive fecal samples, and these isolates represented 17 types of O antigen (O1, O2 or O50, O5, O8, O55, O84, O91, O109, O113, O136, O150, O156, O157, O163, O168, O174, and O177). Two selective media targeting major STEC groups, cefixime-tellurite sorbitol MacConkey agar and CHROMagar O26/O157, allowed isolation of a variety of STEC strains. The most frequently isolated STEC was O113 (8 of 39), which has previously been reported as a cause of foodborne infections. Although most of the O113 STEC isolated from infected patients possessed the enterohemolysin (hlyA) gene, none of the O113 STEC cattle isolates possessed the hlyA gene. The second most common isolate was O157 (6 of 39), and all these isolates contained common virulence factors, including eae, tir, lpf1, lpf2, and hlyA. This study shows the prevalence of O157 and non-O157 STEC in Japanese beef cattle and the relationship of O antigen and virulotypes of the isolates. This information may improve identification of the source of infection, developing surveillance programs or the current understanding of virulence factors of STEC infections.

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BOSILEVAC, JOSEPH M., RONG WANG, BRANDON E. LUEDTKE, SUSANNE HINKLEY, TOMMY L. WHEELER, and MOHAMMAD KOOHMARAIE. "Characterization of Enterohemorrhagic Escherichia coli on Veal Hides and Carcasses." Journal of Food Protection 80, no. 1 (2016): 136–45. http://dx.doi.org/10.4315/0362-028x.jfp-16-247.

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ABSTRACT Enterohemorrhagic Escherichia coli (EHEC) are Shiga toxin–producing E. coli associated with the most severe forms of foodborne illnesses. The U.S. Department of Agriculture, Food Safety and Inspection Service has identified a higher percentage of non-O157 EHEC compared with E. coli O157:H7–positive samples collected from veal trimmings than from products produced from other cattle slaughter classes. Therefore samples were collected from hides and preevisceration carcasses at five veal processors to assess E. coli O157:H7 and non-O157 EHEC contamination during bob veal and formula-fed veal dressing procedures. E. coli O157:H7 prevalence was measured by culture isolation and found to be on 20.3% of hides and 6.7% of carcasses. In contrast, a non-O157 EHEC molecular screening assay identified 90.3% of hides and 68.2% of carcasses as positive. Only carcass samples were taken forward to culture confirmation and 38.7% yielded one or more non-O157 EHEC isolates. The recovery of an EHEC varied by plant and sample collection date; values ranged from 2.1 to 87.8% among plants and from 4.2 to 64.2% within the same plant. Three plants were resampled after changes were made to sanitary dressing procedures. Between the two collection times at the three plants, hide-to-carcass transfer of E. coli O157:H7 and non-O157 EHEC was significantly reduced. All adulterant EHEC serogroups (O26, O45, O103, O111, O121, and O145) were isolated from veal carcasses as well as four other potentially pathogenic serogroups (O5, O84, O118, and O177). Bob veal was found to have a greater culture prevalence of E. coli O157:H7 and greater positive molecular screens for non-O157 EHEC than formula-fed veal (P &lt; 0.05), but the percentage of culture-confirmed non-O157 EHEC was not different (P &gt; 0.05) between the two types of calves. EHEC-O26, -O111, and -O121 were found more often in bob veal (P &lt; 0.05), whereas EHEC-O103 was found more often in formula-fed veal (P &lt; 0.05).

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Elliott, Simon J., Jie Yu, and James B. Kaper. "The Cloned Locus of Enterocyte Effacement from EnterohemorrhagicEscherichia coli O157:H7 Is Unable To Confer the Attaching and Effacing Phenotype upon E. coliK-12." Infection and Immunity 67, no. 8 (1999): 4260–63. http://dx.doi.org/10.1128/iai.67.8.4260-4263.1999.

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ABSTRACT The locus of enterocyte effacement (LEE) pathogenicity island of enterohemorrhagic Escherichia coli (EHEC) O157:H7 possesses the same genes in identical order and orientation as the LEE of enteropathogenic E. coli (EPEC) O127:H6 but is unable to form attaching and effacing (A/E) lesions or to secrete Esp proteins when it is cloned in an E. coli K-12 background. The A/E phenotype could not be restored by trans complementation with a variety of cloned EPEC LEE fragments, suggesting functional and/or regulatory differences between the LEE pathogenicity islands of EPEC O127:H6 and EHEC O157:H7.

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ENACHE, ELENA, EMILY C. MATHUSA, PHILIP H. ELLIOTT, et al. "Thermal Resistance Parameters for Shiga Toxin–Producing Escherichia coli in Apple Juice." Journal of Food Protection 74, no. 8 (2011): 1231–37. http://dx.doi.org/10.4315/0362-028x.jfp-10-488.

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The purpose of the present study was to determine the heat resistance of six non-O157 Shiga toxin–producing Escherichia coli (STEC) serotypes in comparison to E. coli O157:H7 in single-strength apple juice without pulp. The thermal parameters for stationary-phase and acid-adapted cells of E. coli strains from serogroups O26, O45, O103, O111, O121, O145, and O157:H7 were determined by using an immersed coil apparatus. The most heat-sensitive serotype in the present study was O26. Stationary-phase cells for serotypes O145, O121, and O45 had the highest D56°C-value among the six non-O157 serotypes studied, although all were significantly lower (P &lt; 0.05) than that of E. coli O157:H7. At 60°C E. coli O157:H7 and O103 demonstrated the highest D-values (1.37 ± 0.23 and 1.07 ± 0.03 min, respectively). The D62°C for the most heat-resistant strain belonging to the serotype O145 was similar (P &gt; 0.05) to that for the most resistant O157:H7 strain (0.61 ± 0.17 and 0.60 ± 0.09 min, respectively). The heat resistance for stationary-phase cells was generally equal to or higher than that of acid-adapted counterparts. Although E. coli O157:H7 revealed D-values similar to or higher than the individual six non-O157 STEC serotypes in apple juice, the z-values for most non-O157 STEC tested strains were greater than those of E. coli O157:H7. When data were used to calculate heat resistance parameters at a temperature recommended in U.S. Food and Drug Administration guidance to industry, the D71.1°C for E. coli O157:H7 and non-O157 STEC serotypes were not significantly different (P &gt; 0.05).

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More sources

Dissertations / Theses on the topic "E.coli O157"

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Ownis, Ali A. "Verocytotoxin expression by E. coli O157:H7." Thesis, University of Warwick, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343149.

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Silagyi, Karen Suzanne. "Biofilm formation by Escherichia coli O157:H7." College Park, Md.: University of Maryland, 2007. http://hdl.handle.net/1903/7806.

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Thesis (M.S.) -- University of Maryland, College Park, 2007.<br>Thesis research directed by: Dept. of Nutrition and Food Science. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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Cho, Chia-Hui. "Acid tolerance in Escherichia coli O157, H7 following cold shock treatment." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ57527.pdf.

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Ntuli, Victor. "Shigatoxin producing Escherichia coli O157 and non-O157 serotypes in producer-distributor bulk milk." Thesis, University of Pretoria, 2017. http://hdl.handle.net/2263/65932.

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Several recent large outbreaks of gastrointestinal diseases have highlighted the threat posed by morbidity and mortality associated with shigatoxin-producing Escherichia coli (STEC). Furthermore, the treatment of STEC infections is now threatened by the emergence of antibiotic resistance which is an alarming health concern in the world of medicine. The most implicated STEC in foodborne disease outbreaks across the globe is O157 serotype, although some emerging STEC non-O157 serotypes are increasingly becoming recognised as foodborne pathogens of important public health concern. This study was undertaken to characterise bacterial species in raw and pasteurised producer-distributor bulk milk (PDBM) with specific emphasis on E. coli and other Enterobacteriaceae. E. coli was further investigated for the prevalence and distribution of virulence factors (stx 1, stx 2 and hlyA), serotypes and antibiotic resistance patterns, which also included extended-spectrum ?-lactamase (ESBL) producing capacity. Subsequently, the study further estimated the haemolytic uraemic syndrome (HUS) risk associated with the consumption of STEC contaminated PDBM and also estimated the resulting burden of illness that may be associated with the consumption of such milk in South Africa (SA). A total of 258 PDBM samples were collected, using convenience sampling, from outlets (purchase points) in eight different geographical provinces in SA. Isolation, detection and enumeration of total aerobic bacteria, coliforms and E. coli were carried out using 3M E. coli /coliform petrifilm plates. Matrix assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) was used for the rapid identification of the bacterial isolates. The identification of E. coli was confirmed using PCR of the uidA gene. Further characterisation of E. coli into serogroups, identification of virulence factors and antibiotic resistance profiles were then performed. E. coli O157 was characterised using selective media and confirmed using mismatch amplification mutation assay (MAMA)-multiplex PCR. Identification of E. coli -serogroups was carried out by the restriction of amplified O-antigen gene cluster (rfb-RFLP), coupled with serum agglutination assay. Virulence factors (stx 1, stx 2 and hlyA) were determined using both phenotypic and genotypic characterisation. Antimicrobial agent susceptibility tests and detection of extended spectrum beta-lactamase (ESBL) producing capacity of the E. coli isolates were performed using phenotypic characterisation. Finally, a quantitative risk assessment for STEC in PDBM was also conducted. More than 60% of the PDBM samples were found not to be fit for human consumption on the basis of the minimum standards prescribed in the Foodstuffs, Cosmetics and Disinfectants Act (Act 54 of 1972). Raw and pasteurised PDBM was contaminated with a wide diversity of Enterobacteriaceae species, which included spoilage microbiota.<br>Thesis (PhD)--University of Pretoria, 2017.<br>Food Science<br>PhD<br>Unrestricted

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Wales, Andrew Derek. "Studies on Escherichia coli O157:H7 in sheep." Thesis, University of Bristol, 2002. http://hdl.handle.net/1983/63ae6bc6-8e5d-43fb-9ede-594711a03357.

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Escherichia coli 0157: 1-17is a significant human pathogent hat persistsi n asymptomatic animal hosts. It forms a characteristic attachment, the attaching-effacing (AE) lesion, on cell monolayers in vitro and in the intestine in some animal models. Cattle and sheep are asymptomatic carriers of E. coli 0157: H7 and sources of the organism for humans. The present studies examined the persistence of several strains of E. coli 0157: H7 in orally inoculated sheep, and attempted to correlate persistence with features of the strains in vitro and in vivo. A particular hypothesis tested was that adhesion of the bacterium to the intestinal mucosa is a significant mechanism for persistence in animal hosts. Host- and strain-dependentv ariation in the persistente xcretion of E. cola 0157: H7 was observed. Correlations could not be discerned between the persistence of the various bacterial strains and the results of a range of phenotypic tests. The ability of E. coli 0157: H7 to form AE attachments to the large intestinal mucosa in sheep of up to six months of age was demonstrated. No consistent site of persistence of E. coli 0157: H7 within the ovine alimentary tract was found, and AE lesions were not detected in sheep which were persistently excreting the organism. However, commensal bacteria, including E. coli 026, were seen to have formed AE attachments on the large intestinal mucosa. It was concluded that the attachment of E. coli 0157: H7 to the large intestinal mucosa by AE lesion formation may have a role in persistent carriage, but that persistence of the bacterium in the ovine intestine is probably influenced by additional bacterial and host factors. The potential value of interference in the formation of AE lesions, to reduce the prevalence of E. coli 0157: H7 excretion by sheep and other ruminants, merits further investigation.

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Nauer, Timo. "Untersuchungen zum Wachstum und zur Persistenz von Escherichia coli O26:H11, O157:H7, O157:H45 und O159:H~ in Bezug auf Säurestress /." [S.l.] : [s.n.], 2009. http://opac.nebis.ch/cgi-bin/showAbstract.pl?sys=000274968.

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Ungkuraphinunt, Paphapit. "Factors contributing to the presence of Escherichia coli O157:H7 and O157:NM in feedlots and feedlot cattle." Texas A&M University, 2003. http://hdl.handle.net/1969.1/1172.

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Environmental sources within 5 feedlots were sampled for E. coli O157:H7 and O157:NM to determine the prevalence of this pathogen with a view to minimize or control its spread in the feedlot environment. Monthly samples were taken from the feedlots in the Panhandle and South Plains of Texas over a nine-month period. Samples were examined by an immunomagnetic bead separation, followed by plating onto CT-SMAC and CHROMagar O157 media. Sorbitol-negative colonies were tested using ImmunoCard Stat! E. coli O157:H7 Plus and confirmed as E. coli O157:H7, using biochemical (Vitek system) and serological tests (latex agglutination). Additionally, one hundred sponge samples were collected from the hides of stunned cattle at the slaughter plant. All isolates were subjected to rep-PCR DNA fingerprinting and antimicrobial profiling. E. coli O157 was isolated from hide (56%) and environmental samples (4%). E. coli O157 was isolated from all environmental sources, with peak prevalence during November (9%) and March (10%). At least one sample from each feedlot was positive 42% of the time. The most contaminated sites were the chute area (6%) and sludge from waste water ponds (6%). Positive samples were most frequently found from feedlot 5 (7%) and the greatest variation in positive samples between feedlots (0-34%) occurred during March. A decrease in the presence of E. coli O157 in feedlots was observed during January (0%), when ambient, water, and pond sludge temperatures were consistently low. No correlation with other environmental factors was observed. Hide was a primary source of E. coli O157 on carcasses with an overall prevalence of 56%. Of two sampling days, the number of positive hide samples varied from 14% for the first day to 98% for the second day. The total positive samples collected (environmental (47); hide (56)) were 64% H7, and 36% NM. The environmental isolates showed similar antibiotic resistance patterns, regardless of the source. Most E. coli O157 isolates from the feedlots and hides showed a high level of resistance to cephalothin (45%) and sulfisoxazole (56%). E. coli O157 isolates from feedlots were resistant to more than 10 antibiotics (9/317). All of the isolates appeared highly similar, with an average similarity of 53% by rep-PCR DNA fingerprinting.

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Rosser, Tracy. "Pathogenic potential of Escherichia coli O26 and sorbitol-fermenting Escherichia coli O157:NM." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4427.

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Verocytotoxin-producing Escherichia coli (VTEC) are important human pathogens that may cause diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome (HUS). Worldwide, non-sorbitol-fermenting (NSF) VTEC O157:H7 is the most common serogroup associated with HUS but several non-O157:H7 serogroups have emerged as causes of this disease. This research investigated the pathogenic potential of two non-O157:H7 serogroups: O26 and sorbitol-fermenting (SF) O157:NM. While VTEC O26 have emerged as a significant cause of HUS in continental Europe, human infections associated with this pathogen are uncommon in Scotland and generally only result in simple diarrhoea. The study characterised E. coli O26 isolates recovered from human infections in Europe and Scotland and isolates collected from Scottish cattle with the objectives to identify factors which may allow strains to cause more serious clinical disease and to investigate the potential of bovine VTEC O26 in Scotland to cause human infection. MLST analysis of housekeeping genes found little genetic variation in the genomic ‘backbone’ among the vast majority of E. coli O26 isolates. The gene for verocytotoxin 2 (vtx2) alone was carried by VTEC O26 isolates recovered from patients in continental Europe but was found in no Scottish human isolate, where the majority of isolates did not harbour a vtx gene. It was demonstrated that among the European VTEC O26 human isolates, 67% carried a specific allele within the promoter region for LEE1 and 87% harboured the tccP2 gene. In contrast, no Scottish VTEC O26 human isolate carried this allele or the tccP2 gene. The impact these genotypic characteristics have on the pathogenic potential of a strain remains uncertain. There were no clear differences in verocytotoxin titres, levels of LEEencoded protein secretion or levels of adherence to Caco-2 cells between VTEC O26 isolates recovered from human infections of varying severity. However, levels of LEE-encoded protein secretion from cattle isolates were generally higher than those from many of the human isolates. The differences in pathogenic potential between isolates are likely to be due to horizontally acquired DNA, including vtx2 carriage and the O-island-phage-associated effector protein repertoire. Further work is required to determine if the differences identified may also impact on shedding levels from cattle and therefore the likelihood of transmission to humans. Since 1988, SF VTEC O157:NM strains have emerged and have been associated with a higher incidence of progression to HUS than NSF VTEC O157:H7. This study investigated bacterial factors that may account for the increased pathogenic potential of SF VTEC O157:NM. While no evidence of toxin or toxin expression differences between the two VTEC O157 groups was found, the SF VTEC O157:NM strains adhered at significantly higher levels to a human colonic cell line. Under the conditions tested, curli were shown to be the main factor responsible for the increased adherence to Caco-2 cells. The capacity of SF VTEC O157:NM strains to express curli at 37C may have relevance to the epidemiology of human infections as curliated strains could promote higher levels of colonization and inflammation in the human intestine. In turn this could lead to increased toxin exposure and an increased likelihood of progression to HUS.

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Hallewell, Jennyka, and University of Lethbridge Faculty of Arts and Science. "Shiga toxin-producing bacteriophage in Escherichia coli O157:H7." Thesis, Lethbridge, Alta. : University of Lethbridge, Deptartment of Biochemistry, 2008, 2008. http://hdl.handle.net/10133/776.

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Shiga toxin-producing E. coli (STEC) including E. coli O157:H7 are potential food and water borne zoonotic bacterial pathogens capable of causing outbreaks of severe illness in humans. The virulence of E. coli O157:H7 strains may be related to the type of Stx produced and several Stx2 variants have been identified which appear to differ in their ability to cause disease. Two lineages exist within O157 strains where lineage I is associated mainly with human and bovine isolates and lineage II is associated mainly with bovine isolates. The goal of this study was to identify and characterize a lineage II EC970520 Stx2c phage and determine if variations in the phage compared to Stx2 phage found within the lineage I strain, EDL933, can result in differences in virulence observed between the lineages. This study suggests: 1) that the lineage II strain EC970520 contains a highly heterogeneous Stx2c variant phage; 2) that location of integration of the phage within the genome of a bacterium may be important for host selection; 3) that EC970520 Stx2c phage genes are lineage II specific but only a subset of EDL933 phage genes are lineage I specific; 4) that differences in the stability of phages within bacteria contribute to the evolution of new pathogens; 5) that variation in phage genes can be used to detect different strains of E. coli O157:H7 and other STEC; and 6)that the type of phage may result in phenotypic differences between lineages and occurrence of human disease. Results of this study indicate that lineage II strains may be less virulent than lineage I strains due to specific genetic differences and the ability to release phage which is important to the evolution of new pathogenic strains.<br>xv, 162 leaves : ill. ; 29 cm.

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Hopkins, Katie Louise. "Molecular methods for strain characterisation of Escherichia coli O157." Thesis, University of Birmingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368793.

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Books on the topic "E.coli O157"

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Kay, Miller Ellen. Escherichia coli O157. National Agricultural Library, 1993.

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Kay, Miller Ellen. Escherichia coli O157. National Agricultural Library, 1992.

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United States. Animal and Plant Health Inspection Service. Veterinary Services. Centers for Epidemiology and Animal Health., ed. Escherichia coli O157:H7: Issues and ramifications. USDA:APHIS:VS Centers for Epidemiology and Animal Health, 1994.

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Kay, Miller Ellen. Escherichia coli O157: January 1994 - July 1995. National Agricultural Library, 1995.

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United States. Animal and Plant Health Inspection Service. and National Animal Health Monitoring System (U.S.), eds. Escherichia coli O157 in United States feedlots. U.S. Dept. of Agriculture, Animal and Plant Health Inspection Service, 2001.

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Miller, Ellen Kay. Escherichia coli O157: January 1993 - December 1993. National Agricultural Library, 1994.

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United States. Animal and Plant Health Inspection Service. Veterinary Services. Centers for Epidemiology and Animal Health. Escherichia coli O157:H7: Issues and ramifications : executive summary. USDA:APHIS:VS, Centers for Epidemiology and Animal Health, 1994.

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R, Palmer Stephen, ed. E.coli: Environmental health issues of VTEC O157. Spon Press, 2002.

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Urabi, Iftikhar. Virulence factors of verotoxin-producing Escherichia coli O157:H7. typescript, 1993.

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Heersink, Mary. E.coli O157: The true story of a mother's battle with a killer microbe. New Horizon Press, 1996.

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Book chapters on the topic "E.coli O157"

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Griffin, Patricia M., and Thomas G. Boyce. "Escherichia coli O157:H7." In Emerging Infections 1. ASM Press, 2014. http://dx.doi.org/10.1128/9781555816940.ch9.

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Hill, Anne E. "Escherichia Coli O157:H7." In Encyclopedia of Genetics. Routledge, 2014. http://dx.doi.org/10.4324/9781315073972-20.

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Jaros, Patricia, Muriel Dufour, Brent Gilpin, Molly M. Freeman, and Efrain M. Ribot. "PFGE for Shiga Toxin-Producing Escherichia coli O157:H7 (STEC O157) and Non-O157 STEC." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2599-5_15.

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Smith, David R. "Vaccination of Cattle against Escherichia coli O157:H7." In Enterohemorrhagic Escherichia coli and Other Shiga Toxin-Producing E. coli. ASM Press, 2015. http://dx.doi.org/10.1128/9781555818791.ch25.

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Karch, Helge, Andrea Ammon, Phillip I. Tarr, and Martina Bielaszewska. "Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H-." In Population Genetics of Bacteria. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817114.ch16.

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Besser, Thomas E., Margaret A. Davis, and Seth T. Walk. "Escherichia coli O157:H7 in Reservoir Hosts." In Population Genetics of Bacteria. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817114.ch18.

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Laury, Angela, Alejandro Echeverry, and Mindy Brashears. "Fate of Escherichia coli O157:H7 in Meat." In Safety of Meat and Processed Meat. Springer New York, 2009. http://dx.doi.org/10.1007/978-0-387-89026-5_2.

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Lacher, David W. "The Evolutionary Model of Escherichia coli O157:H7." In Population Genetics of Bacteria. ASM Press, 2014. http://dx.doi.org/10.1128/9781555817114.ch13.

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Kendall, Melissa M. "Interkingdom Chemical Signaling in Enterohemorrhagic Escherichia coli O157:H7." In Microbial Endocrinology: Interkingdom Signaling in Infectious Disease and Health. Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-20215-0_9.

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Dean-Nystrom, Evelyn A., Brad T. Bosworth, and Harley W. Moon. "Pathogenesis of Escherichia Coli O157:H7 in Weaned Calves." In Mechanisms in the Pathogenesis of Enteric Diseases 2. Springer US, 1999. http://dx.doi.org/10.1007/978-1-4615-4143-1_16.

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Conference papers on the topic "E.coli O157"

1

Tu, Shu-I., and Andrew Gehring. "Detection of Escherichia coli O157:H7 using immuno beads." In Optics East 2005, edited by Yud-Ren Chen, George E. Meyer, and Shu-I. Tu. SPIE, 2005. http://dx.doi.org/10.1117/12.629949.

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Gao, Yali, Philip M. Sherman, Yu Sun, and Dongqing Li. "Multiplexed High-Throughput Electrokinetically-Controlled Immunoassay on a Chip for the Detection of Specific Bacterial Antibodies in Human Serum." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-42512.

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This work presents a multiplexed electrokinetically-controlled heterogeneous immunoassay that can process ten samples in parallel. The immunoassay microchip was soft-lithographically fabricated using poly(dimethylsiloxane) and glass. Controlling parameters of the electrokinetically-driven flow in the microfluidic network was determined by numerically simulating transport processes. Multiple passively adsorbed antigens captured antibodies present in samples, which then bound with TRITC-labeled detection antibodies to generate fluorescent signals. Antibodies against Escherichia coli O157:H7 and Helicobacter pylori were studied as model analytes. After conditions for antigen-coating were optimized, a 24-minute assay detected E. coli O157:H7 antibody in the concentration range of 0.02–10 μg/mL, and H. pylori antibody in the range of 0.1–50 μg/mL. In testing human serum samples, non-specific binding of serum components was effectively suppressed by using 10% (w/v) bovine serum albumin. An accuracy of 100% was achieved in detecting either E. coli O157:H7 antibody or H. pylori antibody from human serum samples. Simultaneous screening of both antibodies was also successfully demonstrated. The immunoassay chip shows an excellent potential for efficiently detecting multiple pathogenic infections in clinical environments.

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Kaufmann, Martin, Claudio Zweifel, Jorge Blanco, and Roger Stephan. "Prevalence and characteristics of non-O157 shiga toxin-producing Escherichia coli (STEC) and Escherichia coli O157 in fattening pigs at slaughter in Switzerland." In Sixth International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork. Iowa State University, Digital Press, 2005. http://dx.doi.org/10.31274/safepork-180809-775.

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Chen, Hong, Assem Abolmaaty, Peng Li, Constantine Anagnostopoulos, Stefan Du¨bel, and Mohammad Faghri. "Heterogeneous Detection of PCR-Amplified Intimin Gene From E. Coli O157:H7 via PDMS Microfluidic Chip." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-11796.

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E. coli O157:H7 strains represent the most important group of food-borne pathogens. PCR-amplified intimin gene of pathogenic E. coli O157:H7 was detected heterogeneously via a microfluidic chip that consists of streptavidin-coated nanoliter chambers. Biotinylated primers and digoxigenin labeled deoxyuridine triphosphate (dUTP) were incorporated into the amplified intimin (eaeA) gene by an off-chip PCR thermal cycler. The amplified products were injected into the chip where they were immobilized via streptavidin-biotin interaction. Detection of the products using alkaline phosphatase (AP) conjugated anti-digoxigenin was performed with an epi-fluorescent microscope. This assay was capable of detecting 0.06 ng/μL biotin-digoxigenin-dsDNA conjugate distinctly, which is a hundred fold more sensitive than the traditional detection by agarose gel.

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Jung, Byeong Yeal, Suk Chan Jung, Jong Man Kim, Soo Hwan An, and Bong Hwan Kim. "Enzyme immunoassay for the rapid detection of Escherichia coli O157." In Fourth International Symposium on the Epidemiology and Control of Salmonella and Other Food Borne Pathogens in Pork. Iowa State University, Digital Press, 2001. http://dx.doi.org/10.31274/safepork-180809-1196.

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Stephen Radke and Evangelyn Alocilja. "Impedimetric Biosensor for the Rapid Detection of Escherichia coli O157:H7." In 2003, Las Vegas, NV July 27-30, 2003. American Society of Agricultural and Biological Engineers, 2003. http://dx.doi.org/10.13031/2013.14181.

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Tu, Shu-I., Joseph Uknalis, and Andrew Gehring. "Optical methods for detecting Escherichia coli O157:H7 spiked on cantaloupes." In Optics East, edited by Yud-Ren Chen and Shu-I. Tu. SPIE, 2004. http://dx.doi.org/10.1117/12.569269.

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Dujuan Li, Jianping Wang, Yibin YIng, and Yanbin Li. "Rapid Detection of Escherichia coli O157:H7 Using Electrochemical Impedance Immunosensor." In 2007 Minneapolis, Minnesota, June 17-20, 2007. American Society of Agricultural and Biological Engineers, 2007. http://dx.doi.org/10.13031/2013.23116.

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Zakharchenko, A. E., P. A. Domnin, and S. A. Ermolaeva. "STUDY OF THE MECHANISMS OF AUTO-AGGREGATION OF ENTEROPATHOGENIC ESCHERICHIA COLI O157:H7 USING A SYSTEM BASED ON MAGNETIC LEVITATION." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-322.

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The paper compares the autoaggregation ability of the enteropathogenic bacterium Escherichia coli O157:H7 and its transcription factor RcsB mutant using a new system based on magnetic levitation. It has been found that the transcription factor RcsB affects the process of autoaggregation through the control of curli-fimbria.

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Conedera, Gabriella, Massimo Fabbi, Stefano Morabito, Denis Vio, Antonia Ricci, and Alfredo Caprioli. "Isolation of Escherichia coli O157 in pigs at slaughter in Northern Italy." In First International Symposium on the Ecology of Salmonella in Pork Production. Iowa State University, Digital Press, 2007. http://dx.doi.org/10.31274/safepork-180809-22.

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Reports on the topic "E.coli O157"

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Amaya Gómez, Carol Viviana, Liz Alejandra Uribe Gutiérrez, Sabrina del Carmen Jiménez Velásquez, Geraldine Tibasosa Rodriguez, and Deisy Lisseth Toloza Moreno. Actividad antagónica de la colección de mohos del banco de germoplasma de AGROSAVIA frente a bacterias enteropatogénicas resistentes a antibióticos. Corporación colombiana de investigación agropecuaria - AGROSAVIA, 2018. http://dx.doi.org/10.21930/agrosavia.poster.2018.3.

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La colección de mohos del Banco de Germoplasma de Microorganismos de AGROSAVIA (BGMA) tiene actividad biocontroladora contra insectos plaga que afectan cultivos comerciales, sin embargo, existe un limitado conocimiento acerca de su potencial para controlar el crecimiento de enterobacterias como Escherichia coli O157 y Salmonella typhimurium presentes en alimentos contaminados. En este estudio se inició la caracterización de la actividad antagónica de 100 mohos de 25 géneros diferentes, aislados en 16 departamentos de Colombia. Para determinar la actividad antagonista, los mohos y los patógenos se crecieron en medio de ciente en hierro (IDM) y medio R2A. La cepa Mt008 presentó mayor actividad antagónica frente a E. coli O157 en R2A y S. typhimurium en IDM. Mt009 y Nm010 presentaron mayor actividad antagónica frente a E. coli O157 y S. typhimurium en IDM y menor actividad en R2A. Los resultados sugieren que la actividad antagónica exhibida por los mohos podría estar infuenciada por la composición del medio y la presencia del patógeno.

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Ko, Kyung Yuk, Aubrey F. Mendonca, and Dong U. Ahn. EDTA and Lysozyme Improves Antimicrobial Activities of Ovotransferrin against Escherichia coli O157:H7. Iowa State University, 2010. http://dx.doi.org/10.31274/ans_air-180814-1042.

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Clothier, Kris. An Investigation in to the Fecal Shedding of E. Coli O157:H7 from Steers on Rations Containing Corn Co-Products. Iowa State University, 2009. http://dx.doi.org/10.31274/ans_air-180814-876.

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Kudra, Li, Joseph G. Sebranek, James S. Dickson, et al. Controlling Listeria monocytogenes, Campylobactor jejuni, Salmonella enterica Typhimurium and Escherichia coli O157:H7 in Meat Products by Irradiation Combined with Modified Atmosphere Packaging. Iowa State University, 2013. http://dx.doi.org/10.31274/ans_air-180814-13.

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Weinberg, Zwi G., Adegbola Adesogan, Itzhak Mizrahi, Shlomo Sela, Kwnag Jeong, and Diwakar Vyas. effect of selected lactic acid bacteria on the microbial composition and on the survival of pathogens in the rumen in context with their probiotic effects on ruminants. United States Department of Agriculture, 2014. http://dx.doi.org/10.32747/2014.7598162.bard.

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This research project was performed in context of the apparent probiotic effect of selected lactic acid bacteria (LAB) silage inoculants on the performance of ruminants (improved feed intake, faster live-weight gain, higher milk yields and improved feed efficiency). The overall objective was to find out how LAB affect ruminant performance. The project included several “chapters” as follows: 1. The effect of LAB silage inoculants on the survival of detrimental bacteria in rumen fluid, in vitro study (Weinberg et al., The Volcani Center). An in vitro model was developed to study the interaction between selected LAB and an E. coli strain tagged with green fluorescence protein (GFP) in buffered RF. Results indicated that both LAB inoculants and E. coli survived in the RF for several days; both LAB inoculants and LAB-treated silages did not affect survival of E. coli in rumen fluid in vitro. The effect of feeding baled wheat silages treated with or without three selected LAB silage inoculants on the performance of high-lactating cows (Weinberg et al., The Volcani Center). Treatments included control (no additive), Lacobacillusbuchneri40788 (LB), Lactobacillus plantarumMTD1 40027 (LP) and Pediococcuspentosaceus30168 (PP), each applied at 10⁶ cfu/g FM. The silages were included in the TMR of 32 high milking Holstein cows in a controlled feeding experiment. All baled silages were of good quality. The LB silage had the numerically highest acetic acid and were the most stable upon aerobic exposure. The cows fed the LB silages had the highest daily milk yields, percent milk fat and protein. The microbiome of baled wheat silages and changes during ensiling of wheat and corn (Sela et al., The Volcani Center). Bacterial community of the baled silages was dominated mainly of two genera in total, dominated by Lactobacillus and Clostridium_sensu_stricto_12 with 300 other genera at very low abundance. Fungal community was composed mainly of two genera in total, dominated by Candida and Monascuswith 20 other genera at very low abundance. In addition, changes in the microbiome during ensiling of wheat and corn with and without addition of L. plantarumMTD1 was studied in mini-silos. Overall 236 bacterial genera were identified in the fresh corn but after 3 months Lactobacillus outnumbered all other species by acquiring 95% of relative abundance. The wheat silage samples are still under analysis. The effect of applying LAB inoculants at ensiling on survival of E. coli O157:H7 in alfalfa and corn silages(Adesogan et al., University of Florida). E. coli (10⁵ cfu/g) was applied to fresh alfalfa and corn at ensiling with or without L. plantarumor L. buchneri. The pathogen was added again after about 3 moths at the beginning of an aerobic exposure period. The inoculants resulted in faster decrease in pH as compared with the control (no additives) or E. coli alone and therefore, the pathogen was eliminated faster from these silages. After aerobic exposure the pathogen was not detected in the LAB treated silages, whereas it was still present in the E. coli alone samples. 5. The effect of feeding corn silage treated with or without L. buchnerion shedding of E. coli O157:H7 by dairy cows (Adesogan et al., UFL). BARD Report - Project 4704 Page 2 of 12 Five hundred cows from the dairy herd of the University of Florida were screened for E. coli shedding, out of which 14 low and 13 high shedders were selected. These cows were fed a total mixed ration (TMR) which was inoculated with E. coli O157:H7 for 21 days. The TMR included corn silage treated with or without L. buchneri. The inoculated silages were more stable upon aerobic exposure than the control silages; the silage inoculant had no significant effect on any milk or cow blood parameters. However, the silage inoculant tended to reduce shedding of E. coli regardless of high or low shedders (p = 0.06). 6. The effect of feeding baled wheat silages treated with or without three selected LAB silage inoculants on the rumen microbiome (Mizrahi et al., BGU). Rumen fluid was sampled throughout the feeding experiment in which inoculated wheat silages were included in the rations. Microbial DNA was subsequently purified from each sample and the 16S rRNA was sequenced, thus obtaining an overview of the microbiome and its dynamic changes for each experimental treatment. We observed an increase in OTU richness in the group which received the baled silage inoculated with Lactobacillus Plantarum(LP). In contrast the group fed Lactobacillus buchneri(LB) inoculated silage resulted in a significant decrease in richness. Lower OTU richness was recently associated in lactating cows with higher performance (Ben Shabatet al., 2016). No significant clustering could be observed between the different inoculation treatments and the control in non metric multi-dimentional scaling, suggesting that the effect of the treatments is not the result of an overall modulation of the microbiome composition but possibly the result of more discrete interactions. Significant phylum level changes in composition also indicates that no broad changes in taxa identity and composition occurred under any treatment A more discrete modulation could be observed in the fold change of several taxonomic groups (genus level analysis), unique to each treatment, before and after the treatment. Of particular interest is the LB treated group, in which several taxa significantly decreased in abundance. BARD Report - Project 4704 Page 3 of 12

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Ko, Kyung Yuk, Aubrey F. Mendonca, and Dong U. Ahn. Influence of Zn2 + , Sodium Bicarbonate, and Citric Acid on the Antibacterial Activity of Ovotransferrin against E. coli O157:H7 and L. monocytogenes in Model Systems and Ham. Iowa State University, 2010. http://dx.doi.org/10.31274/ans_air-180814-1020.

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