Academic literature on the topic 'E. coli S17-1'

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Journal articles on the topic "E. coli S17-1"

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Di Martino, P., R. Fursy, L. Bret, B. Sundararaju, and R. S. Phillips. "Indole can act as an extracellular signal to regulate biofilm formation of Escherichia coli and other indole-producing bacteria." Canadian Journal of Microbiology 49, no. 7 (2003): 443–49. http://dx.doi.org/10.1139/w03-056.

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We demonstrated previously that genetic inactivation of tryptophanase is responsible for a dramatic decrease in biofilm formation in the laboratory strain Escherichia coli S17-1. In the present study, we tested whether the biochemical inhibition of tryptophanase, with the competitive inhibitor oxindolyl-L-alanine, could affect polystyrene colonization by E. coli and other indole-producing bacteria. Oxindolyl-L-alanine inhibits, in a dose-dependent manner, indole production and biofilm formation by strain S17-1 grown in Luria–Bertani (LB) medium. Supplementation with indole at physiologically r
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Mahapatra, Nitish R., Sajalendu Ghosh, Partha K. Sarkar, and Pataki C. Banerjee. "Generation of Novel Plasmids in Escherichia coli S17-1(pSUP106)." Current Microbiology 46, no. 5 (2003): 318–23. http://dx.doi.org/10.1007/s00284-002-3835-1.

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Folley, L. S., and T. D. Fox. "Reduced dosage of genes encoding ribosomal protein S18 suppresses a mitochondrial initiation codon mutation in Saccharomyces cerevisiae." Genetics 137, no. 2 (1994): 369–79. http://dx.doi.org/10.1093/genetics/137.2.369.

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Abstract A yeast mitochondrial translation initiation codon mutation affecting the gene for cytochrome oxidase subunit III (COX3) was partially suppressed by a spontaneous nuclear mutation. The suppressor mutation also caused cold-sensitive fermentative growth on glucose medium. Suppression and cold sensitivity resulted from inactivation of the gene product of RPS18A, one of two unlinked genes that code the essential cytoplasmic small subunit ribosomal protein termed S18 in yeast. The two S18 genes differ only by 21 silent substitutions in their exons; both are interrupted by a single intron a
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Collet, Anthony, Sébastien Vilain, Pascal Cosette, et al. "Protein expression in Escherichia coli S17-1 biofilms: impact of indole." Antonie van Leeuwenhoek 91, no. 1 (2006): 71–85. http://dx.doi.org/10.1007/s10482-006-9097-3.

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Yee, N., J. Ma, A. Dalia, T. Boonfueng, and D. Y. Kobayashi. "Se(VI) Reduction and the Precipitation of Se(0) by the Facultative Bacterium Enterobacter cloacae SLD1a-1 Are Regulated by FNR." Applied and Environmental Microbiology 73, no. 6 (2007): 1914–20. http://dx.doi.org/10.1128/aem.02542-06.

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ABSTRACT The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global anaerobic regulatory gene fnr (fumarate nitrate reduction regulator) from E. cloacae in the non-Se-reducing strain Escherichia coli S17-1 activated the ability to reduce Se(VI) and precipitate insoluble Se(0) particles. Se(VI) reduction by E. coli S17-1 containing the fnr
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Fen, Chen, Xiong Wei, Min Yong, et al. "Construction of conjugal transfer system of Streptomyces cinnamonensis and effect of PCR-mediated nsdA gene disruption on its secondary metabolism." Chinese Journal of Agricultural Biotechnology 5, no. 1 (2008): 73–79. http://dx.doi.org/10.1017/s1479236208002106.

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AbstractIntergeneric transfer of plasmid vectors pSET152 and pHL212 from donor Escherichia coli ET12567/pUZ8002 and S17-1 to Streptomyces cinnamonensis was demonstrated and optimized. Assisted by this conjugation system, nsdA gene disruption was achieved through PCR-targeted gene replacement. One AprRKanS exconjugant BIB309 was then isolated and confirmed to be the nsdA null mutant. Compared with the starting strain, monensin production by the nsdA− mutant BIB309 increased 270% in vitro.
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Ferrières, Lionel, Gaëlle Hémery, Toan Nham, et al. "Silent Mischief: Bacteriophage Mu Insertions Contaminate Products of Escherichia coli Random Mutagenesis Performed Using Suicidal Transposon Delivery Plasmids Mobilized by Broad-Host-Range RP4 Conjugative Machinery." Journal of Bacteriology 192, no. 24 (2010): 6418–27. http://dx.doi.org/10.1128/jb.00621-10.

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ABSTRACT Random transposon mutagenesis is the strategy of choice for associating a phenotype with its unknown genetic determinants. It is generally performed by mobilization of a conditionally replicating vector delivering transposons to recipient cells using broad-host-range RP4 conjugative machinery carried by the donor strain. In the present study, we demonstrate that bacteriophage Mu, which was deliberately introduced during the original construction of the widely used donor strains SM10 λpir and S17-1 λpir, is silently transferred to Escherichia coli recipient cells at high frequency, bot
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Hoffmann, Andrea, Torsten Thimm, Marcus Dröge, Edward R. B. Moore, Jean Charles Munch, and Christoph C. Tebbe. "Intergeneric Transfer of Conjugative and Mobilizable Plasmids Harbored by Escherichia coli in the Gut of the Soil Microarthropod Folsomia candida(Collembola)." Applied and Environmental Microbiology 64, no. 7 (1998): 2652–59. http://dx.doi.org/10.1128/aem.64.7.2652-2659.1998.

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ABSTRACT The gut of the soil microarthropod Folsomia candidaprovides a habitat for a high density of bacterial cells (T. Thimm, A. Hoffmann, H. Borkott, J. C. Munch, and C. C. Tebbe, Appl. Environ. Microbiol. 64:2660–2669, 1998). We investigated whether these gut bacteria act as recipients for plasmids from Escherichia coli. Filter mating with E. coli donor cells and collected feces of F. candida revealed that the broad-host-range conjugative plasmid pRP4-luc (pRP4 with a luciferase marker gene) transferred to fecal bacteria at estimated frequencies of 5.4 × 10−1 transconjugants per donor. The
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Li, Guojie, and S. Kathariou. "An Improved Cloning Vector for Construction of Gene Replacements in Listeria monocytogenes." Applied and Environmental Microbiology 69, no. 5 (2003): 3020–23. http://dx.doi.org/10.1128/aem.69.5.3020-3023.2003.

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ABSTRACT Listeria monocytogenes is a gram-positive, facultative intracellular bacterium implicated in severe food-borne illness (listeriosis) in humans. The construction of well-defined gene replacements in the genome of L. monocytogenes has been instrumental to several genetic studies of the virulence and other attributes of the organism. Construction of such mutations by currently available procedures, however, tends to be labor intensive, and gene replacement mutants are sometimes difficult to recover due to lack of direct selection for the construct. In this study we describe the construct
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Remus-Emsermann, Mitja N. P., David Aicher, Cosima Pelludat, Pascal Gisler, and David Drissner. "Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids into E. coli O157:H7 on Arabidopsis thaliana Rosettes." Antibiotics 10, no. 8 (2021): 928. http://dx.doi.org/10.3390/antibiotics10080928.

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Many antibiotic resistance genes present in human pathogenic bacteria are believed to originate from environmental bacteria. Conjugation of antibiotic resistance conferring plasmids is considered to be one of the major reasons for the increasing prevalence of antibiotic resistances. A hotspot for plasmid-based horizontal gene transfer is the phyllosphere, i.e., the surfaces of aboveground plant parts. Bacteria in the phyllosphere might serve as intermediate hosts with transfer capability to human pathogenic bacteria. In this study, the exchange of mobilisable and self-transmissible plasmids vi
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Dissertations / Theses on the topic "E. coli S17-1"

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Wan, Hon Wai. "Transformation of the thermophilic bacterium, Geobacillus debilis, by conjugation with the mesophilic bacterium, Escherichia coli." 2013. http://hdl.handle.net/1993/22021.

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A method for transformation of Geobacillus debilis by conjugation was developed using a recombinant plasmid, pNW33N-pxyl-bs2-mob, derived from pNW33N. The plasmid includes the mob region of RP4 for mobilization, is mobilized from E. coli S17-1 to G. debilis, and can stably propagate in G. debilis trans-conjugants grown at 50 oC and 55 oC, in the presence of thiamphenicol. Successful conjugation was depended on the cell density and viability of G. debilis when harvested for conjugation, as well as the metabolic activity of E. coli S17-1 used for conjugation. Substantial reduction in size of the
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