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Journal articles on the topic "E-Steco"
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Frieda, Ani Noor, Prastyoningsih Aris, Wisnu P. Kanita Maria, et al. "Socialization Android-Based Electronic Screening Test Epidemiology (E-Steco 19) In Indonesia and Timor Leste." RA JOURNAL OF APPLIED RESEARCH 09, no. 02 (2023): 66–76. https://doi.org/10.5281/zenodo.7614525.
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<strong>Introduction: </strong>A statement of being in good health is one of the prerequisites as a lecturer or employee at Kusuma Husada University, in which an employee must be declared in good health to prove that an employee is healthy and ready to work, without any serious unhealthy obstacles. Health checks by computerized epidemiology screening test will speed up the process and assist the doctor The development of health applications using the epidemiological screening test method for lecturers and Studends, at the Kusuma Husada University Health Clinic Indonesia Health screening tests quick and efficient in accordance with existing data, and give the result of employee health data directly. The purpose of this study is to make it easier to detect the health status of Kusuma Husada University lecturers and Studends, which is used for one of the prerequisites for a work contract with a health application using an epidemiological screening test method. <strong>Methods: </strong>Data collection was done by qualitative method and carried out from February to August 2022. lecturers and Studends data retrieval is done using a digital questionnaire (online). Sample of this research is a studends, and employee at Kusuma Husada University Indonesia, the results is used to make applications (e-Steco). <strong>Results: </strong>E-Steco 19 is an Android-based application that is used to detect a person's health using the screening test method. Data will be stored using Google Sheet and Google App Script. Thus, the data that has been collected from users who fill out a list of questions in the application can be downloaded and processed according to the needs. Data can be downloaded via the link. The e-Steco result can be processed in such a way that the data can be used as material for decision making. E-Steco 19 consists of 14 questions for health screening. Here we show the results of data processing from e-Steco 19 Result in the form of a pie chart. <strong>Results: </strong>The e-Steco 19 application has been completed and has also been running a test, so that it can make it easier to detect the health of lecturers and Studends, at Kusuma Husada University Indonesia. This application is still possible to be developed and used in Timor Leste University and Health Clinic.
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LIN, ANDREW, LAM NGUYEN, LAURIE M. CLOTILDE, JULIE A. KASE, INSOOK SON, and CAROL R. LAUZON. "Isolation of Shiga Toxin–Producing Escherichia coli from Fresh Produce Using STEC Heart Infusion Washed Blood Agar with Mitomycin-C†." Journal of Food Protection 75, no. 11 (2012): 2028–30. http://dx.doi.org/10.4315/0362-028x.jfp-12-157.
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The ability to detect and isolate Shiga toxin–producing Escherichia coli (STEC) remains a major challenge for food microbiologists. Although methods based on nucleic acids and antibodies have improved detection of STECs in foods, isolation of these bacteria remains arduous. STEC isolation is necessary for matching food, environmental, and clinical isolates during outbreak investigations and for distinguishing between pathogenic and nonpathogenic organisms. STEC heart infusion washed blood agar with mitomycin-C (SHIBAM) is a modification of washed sheep blood agar prepared by adding mitomycin-C and optimizing both the washed blood and base agar to better isolate STECs. Most STEC isolates produce a zone of hemolysis on SHIBAM plates and are easily distinguishable from background microbiota. Here, we present data supporting the use of SHIBAM to isolate STECs from fresh produce. SHIBAM was tested for accuracy in identifying STECs (365 of 410 STEC strains were hemolytic, and 63 of 73 E. coli strains that did not produce Shiga toxin were not hemolytic) and for recovery from artificially inoculated fresh produce (11 of 24 romaine lettuce samples and 6 of 24 tomato samples). STEC recovery with SHIBAM agar was greatly improved when compared with recovery on Levine's eosin–methylene blue agar as a reference method.
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SMITH, JAMES L., and PINA M. FRATAMICO. "Effect of Stress on Non-O157 Shiga Toxin–Producing Escherichia coli†." Journal of Food Protection 75, no. 12 (2012): 2241–50. http://dx.doi.org/10.4315/0362-028x.jfp-12-255.
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Non-O157 Shiga toxin–producing Escherichia coli (non-O157 STEC) strains have emerged as important foodborne pathogens worldwide. Non-O157 STEC serogroups O26, O45, O103, O111, O121, and O145 have been declared as adulterants in beef by the U.S. Department of Agriculture Food Safety and Inspection Service. While documentation is limited, treatments including heat and acid that have been shown to inactivate E. coli O157:H7 will likely also destroy non-O157 STEC; however, non-O157 STEC strains show variability in their responses to stress. It has been shown that non-O157 STEC may survive in fermented sausages and cheeses, and treatments such as high pressure may be necessary to eliminate non-O157 STEC from these products. The mechanisms used by non-O157 STEC to resist acid environments are similar to those used by O157:H7 strains and include the acid tolerance response, the oxidative system, and the glutamate and arginine decarboxylase systems. However, one study demonstrated that some non-O157 STEC strains utilize a chaperone-based acid stress response (HdeA and HdeB) to combat acidic conditions, which is lacking in E. coli O157:H7. Genomic studies suggest that while non-O157 STEC can cause diseases similar to those caused by E. coli O157:H7, O157 and non-O157 STECs have different evolutionary histories. Non-O157 STECs are a heterogeneous group of organisms, and there is currently a limited amount of information on their virulence, fitness, and stress responses, rendering it difficult to draw firm conclusions on their behavior when exposed to stress in the environment, in food, and during processing.
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Wiriyaprom, Ratchakul, Ruttayaporn Ngasaman, Domechai Kaewnoi, and Sakaoporn Prachantasena. "Prevalence and Virulent Gene Profiles of Sorbitol Non-Fermenting Shiga Toxin-Producing Escherichia coli Isolated from Goats in Southern Thailand." Tropical Medicine and Infectious Disease 7, no. 11 (2022): 357. http://dx.doi.org/10.3390/tropicalmed7110357.
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Shiga toxin-producing Escherichia coli (STEC) is the pathogenic E. coli causing disease in humans via the consumption or handling of animal food products. The high prevalence of these organisms in ruminants has been widely reported. Among STECs, O157 is one of the most lethal serotypes causing serious disease in humans. The present study investigated the prevalence of sorbitol non-fermenting STECs in goats reared in the lower region of southern Thailand and described the virulent factors carried by those isolates. Sorbitol non-fermenting (SNF)-STECs were found in 57 out of 646 goats (8.82%; 95% CI 6.75% to 11.28%). Molecular identification revealed that 0.77% of SNF-STEC isolates were the O157 serotype. Shiga toxin genes (stx1 and stx2) and other virulent genes (i.e., eaeA, ehxA, and saa) were detected by molecular techniques. The presence of stx1 (75.44%) was significantly higher than that of stx2 (22.81%), whereas 1.75% of the total isolates carried both stx1 and stx2. Most of the isolates carried ehxA for 75.44%, followed by saa (42.11%) and eaeA (12.28%). In addition, 21.05% of STEC isolates did not carry any eaeA, ehxA, or saa. The first investigation on SNF-STECs in goat was conducted in the lower region of southern Thailand. The present study revealed that goats could be one of the potential carriers of SNF-STECs in the observing area.
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Ghaderi, P., E. Ahmadi, A. M. Farrokhi, et al. "Prevalence and molecular characterisation of Shiga toxin-producing Escherichia coli in sheep farms of Sanandaj, Iran." BULGARIAN JOURNAL OF VETERINARY MEDICINE 27, no. 2 (2024): 206–14. http://dx.doi.org/10.15547/bjvm.2022-0056.
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Shiga toxin-producing Escherichia coli (STEC) strains have emerged as important foodborne pathogens of global public health concern, causing life-threatening diseases. Animals and their products have been documented as important reservoirs for STECs, especially E. coli O157. The aim of this study was to investigate STECs from healthy and diarrhoeic sheep in Sanandaj, Iran. In the current study, a total of 81 sheep faecal samples were taken (22 from diarrhoeic sheep and 59 from healthy sheep). E. coli and subsequently STEC strains was detected according to standard protocol (cultural characterisation and PCR assays). Finally, the frequency of Shiga-toxin producing gene(s) (stx1, stx2), intimin (eaeA) and enterohaemolysin (hlyA) was detected among STEC isolates using duplex PCR. Totally, 42 E. coli were isolated from 81 faecal samples (51.85% contamination). Of these, 34 isolates (80.9%) were identified as STEC patotypes based on Sorbitol-MacConkey (SMAC) medium culturing and also the presence of stx1 and/or stx2. Of these, only 3 isolates (7.1%) were identified as serotype O157:H7 based on PCR assay. In addition, the results showed that STEC bacteria were significantly more prevalent in diarrhoeic samples than in healthy samples (50% vs. 22.1%). Overall, the PCR results showed that 33 (97%), 12 (35.3%) and 8 (23.5%) isolates carried stx1, stx2 and hlyA, respectively. The eaeA gene was not found in any isolate. The number of isolated STEC bacteria in spring (10 isolates) and winter (14 isolates) were significantly higher than those in summer (4 isolates) and autumn (6 isolates) (P=0.039). Also, the number of STEC in diarrhoea samples was significantly higher compared to non-diarrhoea samples (P=0.032). In conclusion, the present study revealed high prevalence rate of STEC including serotype O157:H7 and non-O157:H7 in sheep faeces which highlights the importance of sheep as a reservoir of STEC pathogen in Sanandaj region. Therefore, additional control and preventive measures must be undertaken to control the contamination by this pathogen.
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Nehoya, Kaarina N., Ndinomholo Hamatui, Renatus P. Shilangale, Harris Onywera, Jeya Kennedy, and Lamech M. Mwapagha. "Characterization of Shiga toxin-producing Escherichia coli in raw beef from informal and commercial abattoirs." PLOS ONE 15, no. 12 (2020): e0243828. http://dx.doi.org/10.1371/journal.pone.0243828.
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Shiga toxin-producing Escherichia coli are foodborne pathogens that are mostly associated with beef products and have been implicated in human illness. E.coli-associated illness range from asymptomatic conditions of mild diarrhoea to haemorrhagic colitis which can progress into life threatening haemolytic uremic syndrome (HUS). Beef from cattle are regarded as the main reservoir of Shiga toxin-producing E. coli (STEC) pathogen. The aim of this study was to assess the level and sources of contamination of raw beef with STEC, and determine the incidences of STEC strains in raw beef from informal and commercial abattoirs in Windhoek, Namibia. A total of 204 raw beef samples, 37 equipment and 29 hand swabs were collected and tested for STEC. The meat samples were first enriched with pre-warmed buffered peptone water, cultured on Tryptone Bile X-Glucuronide and CHROMagar STEC, and then sub-cultured on nutrient agar. The presence of E.coli in the samples was confirmed by using VITEK 2 E.coli identification cards and PCR. The overall prevalence of STEC in the meat samples from both the abattoirs was 41.66% raw beef samples; 5.40% equipment swabs; and none of the hand swabs was STEC positive. From the STEC positive meat samples 29.41% contained one of the major STEC strains. Moreover, 52% of the 25 samples that contained the major STECs were characterised by eae and stx1, 8% characterised by eae and stx2 while 40% were characterised by eae, stx1 and stx2 virulence genes. This study has revealed the necessity for proper training on meat safety (for meat handlers) as well as the development, implementation and maintenance of effective sanitary dressing procedures at abattoirs to eliminate beef contamination by STECs thereby ensuring the production of wholesome meat, and to prevent the occurrences of STEC infections.
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Brooks, Dane, Benjamin Bastin, Erin Crowley, et al. "Modification and Matrix Extension of the Bio-Rad iQ-Check E. coli O157:H7, STEC VirX, and STEC SerO Test Kits for the Detection of Shiga Toxin–Producing Escherichia coli (STEC) and Escherichia coli O157 From a Single Enrichment." Journal of AOAC INTERNATIONAL 103, no. 1 (2020): 161–75. http://dx.doi.org/10.5740/jaoacint.19-0254.
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Abstract Background: The iQ-Check Real-Time PCR kits use PCR technology based on gene amplification and detection by a real-time PCR thermalcycler for the detection of target analytes in select food matrices. The iQ-Check E. coli O157:H7 [Performance Tested MethodSM (PTM) 020801] and STEC VirX and STEC SerO (combined PTM 121203) methods were previously validated for different matrices under different enrichment schemes. Objective: To modify the current iQ-Check E. coli O157:H7 Kit for the detection of Escherichia coli O157:H7 from 25 to 375 g for raw ground beef (17% fat), raw beef trim, and fresh spinach. In addition, a matrix extension was validated for iQ-Check E. coli O157:H7 for raw chicken breast without skin (25 g), raw chicken thigh with skin (25 g), mechanically separated chicken (25 g), and raw ground pork (25 g). The study also included the modification of the iQ-Check STEC VirX and SerO Kits for the detection of non-O157 Shiga toxin–producing E. coli (STEC) for raw ground beef (375 g), raw beef trim (375 g), and fresh spinach (375 g) from STEC Enrichment Broth to buffered peptone water (BPW). All tests were carried out at 8–22 h (10–22 h for fresh spinach). Methods: Ground beef, beef trim, and spinach were co-inoculated with E. coli O157:H7, non-O157 STECs, and Salmonella spp. and analyzed for E. coli O157:H7 and non-O157 STECs after an 8-22 h enrichment in BPW for the beef matrices and after a 10–22 h enrichment in BPW for spinach. The chicken matrices were inoculated with E. coli O157:H7 only and analyzed after an 8–22 h enrichment in BPW. The iQ-Check Free DNA Removal Solution workflow was utilized for all matrices. Confirmations at the 22 h time point and method comparisons were conducted with the appropriate reference method as outlined in the U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 4A or the U.S. Department of Agriculture Food Safety and Inspection Service Microbiology Laboratory Guidebook Chapters 5.09 and 5B.05. For the iQ-Check STEC VirX and STEC SerO Kits, inclusivity and exclusivity were also performed. Results: The two inclusivity and exclusivity evaluations indicated that the test methods can accurately detect the target analytes and correctly excluded nontarget organisms after 8 h of enrichment. In the method comparison study, the iQ-Check E. coli O157:H7 and STEC VirX and STEC SerO test kits demonstrated no statistically significant differences between candidate and reference method results or between presumptive and confirmed results for all food matrices analyzed and the two time points (8 or 10 and 22 h). Both time points produced the same results, with no discrepancies. Conclusions: The iQ-Check real-time PCR kits are effective methods for the detection of E. coli O157 and non-O157 STECs (both the virulence factors and the O groups) from raw ground beef, raw beef trim, and fresh spinach in 375 g samples enriched in BPW for 8–22 h (10–22 h for fresh spinach). In addition, the iQ-Check E. coli O157 Kit is effective in detecting E. coli O157 in 25 g samples of raw chicken breast without skin, raw chicken thigh with skin, mechanically separated chicken, and raw ground pork. The iQ-Check test kits allow the end user to pair enrichments for multiple target analytes, allowing the user to prepare a single enrichment and perform a single DNA extraction. The Free DNA Removal Solution removes free DNA from samples prior to PCR analysis, protecting DNA from intact and living cells. Highlights: The method modifications were granted based on the data collected.
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Babolhavaeji, Kiandokht, Leili Shokoohizadeh, Morteza Yavari, Abbas Moradi, and Mohammad Yousef Alikhani. "Prevalence of Shiga Toxin-Producing Escherichia coli O157 and Non-O157 Serogroups Isolated from Fresh Raw Beef Meat Samples in an Industrial Slaughterhouse." International Journal of Microbiology 2021 (December 15, 2021): 1–6. http://dx.doi.org/10.1155/2021/1978952.
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Background. The aims of the current study are the identification of O157 and non-O157 Shiga Toxin-Producing Escherichia coli (STEC) serogroups isolated from fresh raw beef meat samples in an industrial slaughterhouse, determination of antimicrobial resistance patterns, and genetic linkage of STEC isolates. Materials and Methods. A total of 110 beef samples were collected from the depth of the rump of cattle slaughtered at Hamadan industrial slaughterhouse. After detection of E. coli isolates, STEC strains were identified according to PCR for stx1, stx2, eaeA, and hlyA virulence genes, and STEC serogroups (O157 and non-O157) were identified by PCR. The genetic linkage of STEC isolates was analyzed by the ERIC- (Enterobacterial Repetitive Intergenic Consensus-) PCR method. The antimicrobial susceptibility of STEC isolates was detected by the disk diffusion method according to CLSI guidelines. Results. Among 110 collected beef samples, 77 (70%) were positive for E. coli. The prevalence of STEC in E. coli isolates was 8 (10.4%). The overall prevalence of O157 and non-O157 STEC isolates was 12.5% (one isolate) and 87.5% (7 isolates), respectively. The hemolysin gene was detected in 25% (2 isolates) of STEC strains. Evaluation of antibiotic resistance indicated that 100% of STEC isolates were resistant to ampicillin, ampicillin-sulbactam, amoxicillin-clavulanic acid, and cefazolin. Resistance to tetracycline and ciprofloxacin was detected in 62.5% and 12.5% of isolates, respectively. The analysis of the ERIC-PCR results showed five different ERIC types among the STEC isolates. Conclusion. The isolation of different clones STECs from beef and the presence of antibiotic-resistant isolates indicate that more attention should be paid to the hygiene of slaughterhouses.
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Yang, Xi, Yannong Wu, Qian Liu, et al. "Genomic Characteristics of Stx2e-Producing Escherichia coli Strains Derived from Humans, Animals, and Meats." Pathogens 10, no. 12 (2021): 1551. http://dx.doi.org/10.3390/pathogens10121551.
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Shiga toxin (Stx) can be classified into two types, Stx1 and Stx2, and different subtypes. Stx2e is a subtype commonly causing porcine edema disease and rarely reported in humans. The purpose of this study was to analyze the prevalence and genetic characteristics of Stx2e-producing Escherichia coli (Stx2e-STEC) strains from humans compared to strains from animals and meats in China. Stx2e-STEC strains were screened from our STEC collection, and whole-genome sequencing was performed to characterize their genetic features. Our study showed a wide distribution of Stx2e-STEC among diverse hosts and a higher proportion of Stx2e-STEC among human STEC strains in China. Three human Stx2e-STEC isolates belonged to O100:H30, Onovel26:H30, and O8:H9 serotypes and varied in genetic features. Human Stx2e-STECs phylogenetically clustered with animal- and food-derived strains. Stx2e-STEC strains from animals and meat showed multidrug resistance, while human strains were only resistant to azithromycin and tetracycline. Of note, a high proportion (55.9%) of Stx2e-STEC strains, including one human strain, carried the heat-stable and heat-labile enterotoxin-encoding genes st and lt, exhibiting a STEC/enterotoxigenic E. coli (ETEC) hybrid pathotype. Given that no distinct genetic feature was found in Stx2e-STEC strains from different sources, animal- and food-derived strains may pose the risk of causing human disease.
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Maguire, Meghan, Julie A. Kase, Dwayne Roberson, et al. "Precision long-read metagenomics sequencing for food safety by detection and assembly of Shiga toxin-producing Escherichia coli in irrigation water." PLOS ONE 16, no. 1 (2021): e0245172. http://dx.doi.org/10.1371/journal.pone.0245172.
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Shiga toxin-producing Escherichia coli (STEC) contamination of agricultural water might be an important factor to recent foodborne illness and outbreaks involving leafy greens. Closed bacterial genomes from whole genome sequencing play an important role in source tracking. We aimed to determine the limits of detection and classification of STECs by qPCR and nanopore sequencing using 24 hour enriched irrigation water artificially contaminated with E. coli O157:H7 (EDL933). We determined the limit of STEC detection by qPCR to be 30 CFU/reaction, which is equivalent to 105 CFU/ml in the enrichment. By using Oxford Nanopore’s EPI2ME WIMP workflow and de novo assembly with Flye followed by taxon classification with a k-mer analysis software (Kraken2), E. coli O157:H7 could be detected at 103 CFU/ml (68 reads) and a complete fragmented E. coli O157:H7 metagenome-assembled genome (MAG) was obtained at 105−108 CFU/ml. Using a custom script to extract the E. coli reads, a completely closed MAG was obtained at 107−108 CFU/ml and a complete, fragmented MAG was obtained at 105−106 CFU/ml. In silico virulence detection for E. coli MAGs for 105−108 CFU/ml showed that the virulotype was indistinguishable from the spiked E. coli O157:H7 strain. We further identified the bacterial species in the un-spiked enrichment, including antimicrobial resistance genes, which could have important implications to food safety. We propose this workflow provides proof of concept for faster detection and complete genomic characterization of STECs from a complex microbial sample compared to current reporting protocols and could be applied to determine the limit of detection and assembly of other foodborne bacterial pathogens.
Dissertations / Theses on the topic "E-Steco"
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Harder, Jörn [Verfasser], and E. [Akademischer Betreuer] Steck. "Simulation lokaler Fließvorgänge in Polykristallen / Jörn Harder ; Betreuer: E. Steck." Braunschweig : Technische Universität Braunschweig, 1997. http://d-nb.info/117589141X/34.
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Sporrong, Oscar. "Improved methodology for isolating Shiga toxin-producing E. coli (STEC) in food." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-357314.
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Paddock, Zachary Dean. "Shiga toxin producing Escherichia coli (STEC) in cattle: factors affecting fecal shedding of E. coli O157:H7 and detection methods of non-O157 STEC." Diss., Kansas State University, 2013. http://hdl.handle.net/2097/15732.
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Doctor of Philosophy<br>Department of Diagnostic Medicine/Pathobiology<br>T. G. Nagaraja<br>Escherichia coli O157:H7 and over 380 non-O157 serotypes of Shiga toxin producing E. coli (STEC) are human food-borne pathogens that inhabit the hindgut of ruminants and are shed in the feces, which subsequently contaminate food products. Recent epidemiological data have shown that six non-O157 STEC (O26, O103, O111, O121, O45 and O145) account for majority of human STEC infections. Fecal shedding of STEC is influenced by a number of factors, including diets, supplements, and feed additives, because of their potential to alter hindgut ecosystem. Not much is known about the fecal shedding of non-O157 STEC in cattle because of lack of standardized detection methods. Fecal shedding of E. coli O157:H7 was studied to determine the effects of supplemental urea, monensin, an ionophore, and ractopamine, a beta-agonist. Cattle fed monensin at 44 mg/kg of feed had lower (P = 0.05) fecal O157:H7 prevalence than cattle fed 33 mg/kg. Supplemental urea (0.35 or 0.70% of the diet) and inclusion of ractopamine at 200 mg/animal/day had no effect on fecal shedding of E. coli O157:H7. In an experimental inoculation study, inclusion of corn starch to a distiller’s grains (DG)-supplemented diet had no effect on fecal shedding of E. coli O157 suggesting that either the decreased starch content in the DG-supplemented diet is not a factor in the increased shedding of E. coli O157:H7 or inclusion of pure starch in the diet may not have achieved our intended goal to have starch flow into the hindgut similar to that of corn grain. A multiplex PCR to detect O26, O45, O103, O111, O121, O145, and O157 was designed and applicability to detect the seven serogroups in cattle feces was evaluated. A multiplex PCR, designed to detect E. coli O104, feces showed presence of O104 in cattle feces (20.6%), but the isolated strains did not carry genes characteristic of the virulent strain responsible for the 2011 food-borne outbreak in Germany. Two preharvest interventions, a siderophore receptor and porin proteins-based vaccine and a Lactobacillus acidophilus-based direct-fed microbial, intended to control E. coli O157, had no effect on fecal shedding of O26 assessed by culture-based or PCR-based method.
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Smith, Victor, Ankit Patel, Emily Ford, Demetrio M. D. Macariola, and Alex Yu. "Shigatoxin E. coli (STEC) in Public Park at Different Seasons of the Year." Digital Commons @ East Tennessee State University, 2020. https://dc.etsu.edu/asrf/2020/presentations/56.
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BACKGROUND:
In the U.S. STEC HUS is the most common cause of acute renal failure in children. In TN from 1996-2017 there were 2008 STEC cases were reported. Every year in the U.S, there 36 reported mortality each year. At our local children’s hospital, 4-5 children are hospitalized with STEC infection each year. Some of these children had no history of ingesting food items that could have placed them at risk to develop STEC infection; however, there are other ways that humans could get infected, such as exposure to contaminated water from cattle farms.
GOALS:
To determine if there are differences in the presence of STEC at a local park at different seasons of the year.
METHODS:
Fifty (50) ml of water samples were collected from a creek in 2 areas of public park in Johnson City, TN. Samples were inoculated to Sorbitol McConkey Agar (SMAC) plates under sterile techniques & incubated at 36C for 18 hours under aerobic conditions.
RESULTS: Table demonstrating presence of STEC from water samples at different seasons of the year.
SEASON OF THE YEAR
# COLONIES FOUNDERS PARK
# COLONIES LIBRARY PARK
SUMMER JUNE 2018
A:1 B:1 C:2
TOTAL: 4
A: 3 B: 2 C: 1
TOTAL: 6
FALL SEPT 2018
A: 1 B: 3 C: 2
TOTAL: 6
A: 1 B:2 C:4
TOTAL: 7
WINTER DEC 2018
A: 1 B: 0 C: 1
TOTAL: 2
A: 0 B: 1 C: 1
TOTAL: 2
SPRING MARCH 2019
A: 2 B: 2 C:1
TOTAL: 5
A: 0 B: 0 C: 0
TOTAL: 0
DISCUSSION/ CONCLUSION:
STEC was present at almost every season of the year. Public health measures should be undertaken to inform the community that these waters around public parks are contaminated with STEC to prevent STEC infection.
References: TN Dept of Health CEDEP report CDC website
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Cardozo, Marita Vedovelli [UNESP]. "Detecção de Escherichia coli shigatoxigênica (STEC) e enteropatogênica (EPEC) em peixes de pisciculturas e de vida livre." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/122015.
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000817299.pdf: 639613 bytes, checksum: 3a27811ced1e1d8bc5861bd392515794 (MD5)<br>Embora Escherichia coli não seja um microrganismo natural do trato intestinal de peixes, sabe-se que a microbiota desses animais está diretamente relacionada à qualidade microbiológica da água em que vivem. Para avaliar a frequência de Escherichia coli shigatoxigênica (STEC) e enteropatogênica (EPEC), foram coletadas um total de 472 amostras de fezes e musculatura de peixes da espécie Oreochromis niloticus (tilápia), bem como da água em que viviam. Nos animais provenientes de pisciculturas, somente uma estirpe STEC (0,2%) foi isolada, e esta apresentou resistência a diversos antimicrobianos das classes das quinolonas, tetraciclinas, aminoglicosídeos e anfenicóis, enquanto em peixes de vida livre, seis estirpes (6%) foram isoladas, sendo cinco STEC e uma EPECa. Todas as STEC foram classificadas como patogênicas pelo grupamento filogenético e a EPECa, comensal. Além disso, foram detectados os genes de virulência astA, ehxA, saa, efa1, paa e lpfAO113 e os sorogrupos O55, O39, O116, H14, H18 e H36. Em relação às variantes da toxina Stx2, foi observada a rara combinação dos subtipos stx2a, stx2c e stx2d em um mesmo isolado. De acordo com a análise de similaridade genética, os isolados são bastante heterogêneos e apresentaram origem clonal diversificada. Os resultados desse estudo evidenciaram que os peixes têm o potencial de transmissão de E. coli diarreiogênicas para o ser humano, e também o possível uso indiscriminado de antimicrobianos na criação intensiva desses animais<br>Although Escherichia coli is not an organism typically found in the fish gut, the microbiota of these animals is directly related to the quality of water in which they live. To evaluate the frequency of shigatoxigenic (STEC) and enteropathogenic (EPEC) Escherichia coli, a total of 472 fish fecal and muscle samples were collected (Oreochromis niloticus, called tilapia), as well as the water in which the fish lived. In animals from fish farms, only one STEC strain (0.2%) was isolated, and showed resistant to several antimicrobial of the quinolone, tetracycline, aminoglycoside and amphenicol classes. In wild fish, six isolates (6%) were obtained, five STEC and one aEPEC. All STEC were classified as pathogenic by phylogenetic grouping, and aEPEC was commensal. In addition, astA, ehxA, saa, efa1, paa, lpfAO113 virulence genes, and O55, O39, O116, H14, H18 and H36 serogroups were detected. Regarding Stx2 toxin variants, an unusual combination of stx2a, stx2c and stx2d subtypes was observed. According to the analysis of genetic similarity, the isolates are heterogeneous and showed a diversified clonal origin. The results of this study demonstrated that fish have the potential to transmit diarrheagenic E. coli to humans, as well as the possible indiscriminate use of antimicrobials in the intensive farming of these animals
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Cardozo, Marita Vedovelli. "Detecção de Escherichia coli shigatoxigênica (STEC) e enteropatogênica (EPEC) em peixes de pisciculturas e de vida livre /." Jaboticabal, 2014. http://hdl.handle.net/11449/122015.
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Orientador: Fernando Antônio de Ávila<br>Banca: Caroline Peters Pigatto de Nardi<br>Banca: Patrícia Amoroso<br>Banca: Helio José Montassier<br>Banca: José Moacir Marin<br>Resumo: Embora Escherichia coli não seja um microrganismo natural do trato intestinal de peixes, sabe-se que a microbiota desses animais está diretamente relacionada à qualidade microbiológica da água em que vivem. Para avaliar a frequência de Escherichia coli shigatoxigênica (STEC) e enteropatogênica (EPEC), foram coletadas um total de 472 amostras de fezes e musculatura de peixes da espécie Oreochromis niloticus (tilápia), bem como da água em que viviam. Nos animais provenientes de pisciculturas, somente uma estirpe STEC (0,2%) foi isolada, e esta apresentou resistência a diversos antimicrobianos das classes das quinolonas, tetraciclinas, aminoglicosídeos e anfenicóis, enquanto em peixes de vida livre, seis estirpes (6%) foram isoladas, sendo cinco STEC e uma EPECa. Todas as STEC foram classificadas como patogênicas pelo grupamento filogenético e a EPECa, comensal. Além disso, foram detectados os genes de virulência astA, ehxA, saa, efa1, paa e lpfAO113 e os sorogrupos O55, O39, O116, H14, H18 e H36. Em relação às variantes da toxina Stx2, foi observada a rara combinação dos subtipos stx2a, stx2c e stx2d em um mesmo isolado. De acordo com a análise de similaridade genética, os isolados são bastante heterogêneos e apresentaram origem clonal diversificada. Os resultados desse estudo evidenciaram que os peixes têm o potencial de transmissão de E. coli diarreiogênicas para o ser humano, e também o possível uso indiscriminado de antimicrobianos na criação intensiva desses animais<br>Abstract: Although Escherichia coli is not an organism typically found in the fish gut, the microbiota of these animals is directly related to the quality of water in which they live. To evaluate the frequency of shigatoxigenic (STEC) and enteropathogenic (EPEC) Escherichia coli, a total of 472 fish fecal and muscle samples were collected (Oreochromis niloticus, called tilapia), as well as the water in which the fish lived. In animals from fish farms, only one STEC strain (0.2%) was isolated, and showed resistant to several antimicrobial of the quinolone, tetracycline, aminoglycoside and amphenicol classes. In wild fish, six isolates (6%) were obtained, five STEC and one aEPEC. All STEC were classified as pathogenic by phylogenetic grouping, and aEPEC was commensal. In addition, astA, ehxA, saa, efa1, paa, lpfAO113 virulence genes, and O55, O39, O116, H14, H18 and H36 serogroups were detected. Regarding Stx2 toxin variants, an unusual combination of stx2a, stx2c and stx2d subtypes was observed. According to the analysis of genetic similarity, the isolates are heterogeneous and showed a diversified clonal origin. The results of this study demonstrated that fish have the potential to transmit diarrheagenic E. coli to humans, as well as the possible indiscriminate use of antimicrobials in the intensive farming of these animals<br>Doutor
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Tawe, Johanna. "Validation of PCR assays for detection of Shiga toxin-producing E. coli O104:H4 and O121 in food." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-220478.
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Shigatoxin-producing Escherichia coli (STEC) can cause infections in humans which can beserious and sometimes fatal. There is a great need for methods that are able to detect differentserogroups of STEC. In this project, conventional and real-time PCR assays for detection ofSTEC O104:H4 and O121, as recommended by the European Union Reference Laboratory(EU-RL) for STEC, were validated. The specificity, limit of detection, repeatability,efficiency and robustness were determined for three real-time PCR assays. The validationshowed that the real-time PCR reactions were specific and sensitive although some additionaltests are required.
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Machado, Luís Alberto Pereira. "Incidência dos genes eaeA E stx1 EM Escherichia coli isolada de carcaça suína abatida em frigoríficos comercais na região sul do Brasil." reponame:Repositório Institucional da UNIVATES, 2014. http://hdl.handle.net/10737/600.
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2014LuisAlbertoPereiraMachado.pdf: 1981082 bytes, checksum: 5d67114fb9c3571e082ce983723758e1 (MD5)<br>A carne suína representa importante fonte de proteína animal para o mundo, porém vem sendo associada a surtos de toxinfecção alimentar. Uma das causas destes surtos é a contaminação por Escherichia coli (E. coli), encontrada no trato intestinal e ambiente dos suínos abatidos para produção de carnes in natura e industrializadas. No contexto de segurança alimentar, os surtos por Escherichia coli produtoras de toxina Shiga (STEC) são os melhores documentados. No mundo, diversos surtos causados por ingestão de alimentos de origem animal já foram documentados, porem no Brasil, existem poucos dados sobre a ocorrência de STEC em eventos de toxinfecção alimentar, bem como a presença nos animais e, consequentemente, na carne suína. O objetivo deste trabalho foi quantificar a contaminação por E. coli de carcaças suínas abatidos em frigoríficos comerciais localizados nos estados da Região Sul do Brasil, e identificar por Reação em Cadeia da Polimerase (PCR) a presença de E. coli produtora das toxinas stx1 e eaeA. Foram realizados swabs de 272 carcaças suínas em abatedouros frigoríficos localizados nos estados do RS, SC e PR. As contaminações por E. coli foram identificadas em 25 carcaças, sendo 20 estabelecimento do Rio Grande do Sul, cinco no do Paraná e nenhuma amostra positiva na coleta em Santa Catarina. Das amostras positivas foram extraídos DNA para genotipagem por PCR. Nenhuma amostra apresentou o gene stx1, porém o gene eaeA foi identificado em 13 amostras, nas diferentes regiões da carcaça. A identificação da incidência dos genes eaeA e stx1 nas carcaças pela técnica de PCR pode ser uma ferramenta útil no rastreamento da contaminação bacteriana ao longo dos processos do abatedouro, podendo auxiliar na redução de casos de toxinfecções alimentares causadas por E. coli e outros microrganismos.
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Cull, Charley Abraham. "Epidemiology of Shiga toxin-producing Escherichia coli in commericial feedlot cattle." Diss., Kansas State University, 2016. http://hdl.handle.net/2097/32571.
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Doctor of Philosophy<br>Diagnostic Medicine/Pathobiology<br>David G. Renter<br>Shiga toxin-producing Escherichia coli serogroups (O26, O45, O103, O111, O121, O145, and O157; STEC-7) are recognized as major food-borne pathogens with outbreaks, human infections, and occasional deaths associated with the consumption of contaminated foods. Cattle are recognized as the primary reservoir for STEC-7 and shed these bacteria in their feces, which are considered a principal source of contamination of cattle hides and carcasses at harvest. Pre-harvest interventions that effectively reduce fecal shedding of STEC-7 have the potential to reduce the public health concerns and economic impact of these bacteria and enhance food safety. In the research presented in this dissertation, distinct study designs were used to evaluate the impact of commercially available pre-harvest interventions and develop a better understanding of the epidemiology of STEC-7 in commercial feedlot cattle. A randomized pen-level trial indicated that a commercially available vaccine significantly reduced the fecal prevalence of STEC O157 and prevalence of high shedders compared to unvaccinated pens. However, there was no evidence of a direct-fed microbial (DFM) effect on either measure of STEC O157 shedding. In a continuum of the efficacy study, the performance and carcass characteristics associated with these pre-harvest interventions were quantified. Results indicated that feeding the DFM to cattle improved performance, whereas the vaccine negatively impacted performance during the intervention period, though most of these attributes were not reflected at the time the animals were harvested. Later, a cross-sectional observational study was used to determine the regional-, feedlot- and pen-level fecal prevalence of enterohemorrhagic Escherichia coli (EHEC), a subset of STEC, in commercial feedlot cattle. Results indicated that EHEC serogroup O157 was detected more frequently than non-O157 serogroups of EHEC; however, all feedlots had at least one sample positive for both O157 and non-O157 EHEC. Further, risk factors associated with non-O157 serogroups of EHEC were identified; further evaluation of these factors as potential control points may enable the ability to positively impact public health concerns and food safety by reducing the pathogen load prior to harvest. Overall, the research described in this dissertation provides an assessment of pre-harvest interventions and multi-level prevalence estimates of STEC-7 in commercial feedlot operations.
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Correa, Taciana Pretti [UNESP]. "Persistência de cepas de Escherichia coli genérica e de Stec não-O157 em solos agricultáveis." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/136753.
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000860193.pdf: 914175 bytes, checksum: f8f884f6f45106b6f91e035fd147770d (MD5)<br>Escherichia coli de sorogrupo não- O157 produtora de Shiga toxina é reconhecida como causadora de sérias doenças em seres humanos. Entretanto, as pesquisas relacionadas à persistência das STEC não- O157 em solos agricultáveis são bastante limitadas. No presente estudo foi analisada a sobrevivência de E.coli genérica, E.coli portadora do gene eae e STEC não- O157 portadoras do gene stx1, stx2 e eae em dois solos agricultáveis, cultura de cana-de-açúcar e pastagem em duas situações diferentes, solos esterilizados por autoclavação e solo não autoclavado. As linhagens de E.coli foram inoculadas nos dois solos em ambas as situações em um sistema de microcosmo, o qual foi avaliado por 60 dias. Os resultados mostraram que todas as linhagens de E.coli foram recuperadas em menor número nos solos não autoclavados do que nos solos esterilizados por autoclavação. Também foi verificado que as linhagens STEC não- O157 sobreviveram por um período maior do que a linhagem de E.coli genérica no solo sob pastagem não autoclavado, no mínimo por 60 dias. Nós devemos prestar mais atenção ao longo período de sobrevivência das linhagens STEC não- O157 em solos de pastagem devido ao seu alto potencial de risco ao meio ambiente<br>Shiga toxin producing Escherichia coli non- O157 serogroup has been recognized as cause of serious diseases in human. However, research on the persistence of STEC non- O157 in preharvest is limited. In the current study we analysed the survival behavior of generic E.coli, E.coli carrying eae gene and STEC non- O157 strain carrying stx1, stx2 and eae gene in two agricultural soils, sugar cane culture and grassland in two different situations autoclaved soil and non- autoclaved soil. The E.coli strains were inoculated in both soils in a microcosm system which was analysed for sixty days. Results showed that all E.coli strains were recovered in a smoller number in non- autoclaved soils than in the autoclaved soils. Also was verified that the STEC non- O157 strains survived longer than the generic E.coli strain in the non autoclaved grassland soil, at least for sixty days. We should pay more attention to the STEC non- O157 serogroup long survival in grassland soil and its potential environmental risk
Books on the topic "E-Steco"
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VAUGHN, STECK. Steck-vaughn onramp approach crossroads: Student reader the plight of e. hammond. Steck-Vaughn Co, 2007.
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VAUGHN, STECK. Facts: Level E (Steck-Vaughn Comprehension Skills). Tandem Library, 1992.
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VAUGHN, STECK. Conclusion: Level E (Steck-Vaughn Comprehension Skills). Tandem Library, 1992.
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VAUGHN, STECK. Inference: Level E (Steck-Vaughn Comprehension Skills). Tandem Library, 1992.
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Beech, Linda. Sequence: Level E (Steck-Vaughn Comprehension Skills). Steck-Vaughn, 1999.
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VAUGHN, STECK. Conclusion: Level E (Steck-Vaughn Comprehension Skills). Tandem Library, 1992.
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VAUGHN, STECK. Conclusion: Level E (Steck-Vaughn Comprehension Skills). Tandem Library, 1992.
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VAUGHN, STECK. Critical Thinking: Level E (Critical Thinking (Steck-Vaughn)). Tandem Library, 1993.
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Chin, James, Sam Abraham, Ren Zhang, and Rafat Al-Jassim. "Diarrheagenic Escherichia coli Pathotypes (DEP) Including Enterohaemorrhagic (EHEC)/Shiga-toxin E. coli (STEC)." In PCR for Clinical Microbiology. Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9039-3_15.
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Oliveira, MS, BP Dias, CA Nascimento, IGC Santos, JC Ribeiro Júnior, and B. Alexandrino. "STEC E EHEC EM QUEIJOS FRESCAIS PRODUZIDOS COM LEITE CRU COMERCIALIZADOS NO NORTE DO TOCANTINS." In ANAIS DO III CONGRESSO REGIONAL DE SANIDADE ANIMAL E SAÚDE PÚBLICA E XIII SEMANA ACADÊMICA DE MEDICINA VETERINÁRIA DA UFT - 2020. Wissen Editora, 2020. http://dx.doi.org/10.52832/wed.36.189.
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Hall, Gregory, Shinichiro Kurosawa, and D. J. Stearns-Kurosawa. "Infection of Immunocompetent Conventional Mice with Shiga Toxin-Producing E. coli: The DSS + STEC Model." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1339-9_17.
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Costa, Cristiane Mara Silva da, Adriana Hamond Regua Mangia, Alice Gonçalves Martins Gonzalez, Bruno Gomes de Castro, and Denis Yukio Otaka. "CARACTERIZAÇÃO MOLECULAR DE AMOSTRAS DE ESCHERICHIA COLI CARREADORAS DOS GENES STX ISOLADAS DE BOVINOS DE REGIÕES AGROPECUÁRIAS BRASILEIRAS: PARTE II." In Pesquisas Aplicadas à Saúde Coletiva. Bookerfield Editora, 2021. http://dx.doi.org/10.53268/bkf21091507.
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Escherichia coli produtora da toxina Shiga (STEC) é um microrganismo agente de infecções de amplo espectro clínico incluindo desde quadros assintomáticos, casos diarreicos leves até doenças extra intestinais graves caracterizadas por anemia hemolítica microangiopática, trombocitopenia e insuficiência renal aguda. Além da produção de shigatoxinas, a expressão de intimina e a produção de enterohemolisinas são atributos de virulência também descritos no grupo patogênico. Ruminantes, especialmente bovinos, são reconhecidos como o principal reservatório das STEC e o manejo inadequado desses animais pode representar um risco de contágio e de disseminação desses patógenos. O estudo incluiu 301 isolados de E. coli obtidos de amostras fecais de bovinos, com e sem diarreia, de regiões agropecuárias brasileiras no Rio de Janeiro e em Rondônia. As amostras bacterianas foram caracterizadas quanto ao polimorfismo genético e a presença de marcadores genéticos codificadores de virulência e dos grupos antigênicos associados ao patotipo. Ensaios moleculares revelaram que 55,5% (167/301) das amostras de E. coli foram carreadoras do gene stx, 36,2% (109/301) e 21,3% (64/301) dos genes eae+ e ehxA+, respectivamente. Dentre as STEC, 24 foram isoladas do Rio de Janeiro (14,4%, 24/167) e 143 de Rondônia (85,6%, 143/167). 13 genótipos de virulência foram observados: stx1 /stx2 /eae/ehxA, stx1 /stx2 /eae, stx1 /stx2 /ehxA, stx1 /stx2 , stx1 / eae/ehxA, stx1 /ehxA, stx1 , stx2 /eae/ehxA, stx2 /eae, stx2 /ehxA, stx2 , eae/ehxA e ehxA). 3,6% (6/167) e 93,4% (156/167) das STEC foram carreadoras dos genes rfbO113 e rfbO157+, respectivamente, sendo 64,1% desses rfbO157+h7+ (100/156). A tipagem da região LEE revelou os subtipos eaeα (3/109), eaeβ (6/109), eaeγ (4/109), tirα (2/109), tirβ (3/109), tirγ (3/109), espAα (3/109), espAβ (0/109), espAγ (0/109), espBα (1/109), espBβ (9/109) e espBγ (0/109). A filogrupagem classificou as STEC nos grupos A (44,9%), B1 (47,3%), D (6,6%) e B2 (1,2%). A tipagem pelo RAPD- revelou uma ampla diversidade genética entre as STEC indicando constituir uma população bacteriana de origem não clonal. A elevada prevalência de STEC em bovinos clinicamente sadios confirma o seu papel como importantes reservatórios ambientais. A ampla circulação desses patógenos em animais de propriedades rurais, em especial, aquelas cujas condições de infraestrutura e boas práticas de manejo são insatisfatórias, representa um sério risco ambiental de concentração desses microrganismos, propiciando a exposição e a ocorrência de doenças e das demais manifestações clínicas relacionadas ao patógeno. O estudo é dividido em duas partes, sendo que o primeiro capítulo apresenta os aspectos introdutórios e metodológicos e por meio do segundo são mostrados os resultados, discussão e conclusões.
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Costa, Cristiane Mara Silva da, Adriana Hamond Regua Mangia, Alice Gonçalves Martins Gonzalez, Bruno Gomes de Castro, and Denis Yukio Otaka. "CARACTERIZAÇÃO MOLECULAR DE AMOSTRAS DE ESCHERICHIA COLI CARREADORAS DOS GENES STX ISOLADAS DE BOVINOS DE REGIÕES AGROPECUÁRIAS BRASILEIRAS: PARTE I." In Pesquisas Aplicadas à Saúde Coletiva. Bookerfield Editora, 2021. http://dx.doi.org/10.53268/bkf21091506.
Full textAbstract:
Escherichia coli produtora da toxina Shiga (STEC) é um microrganismo agente de infecções de amplo espectro clínico incluindo desde quadros assintomáticos, casos diarreicos leves até doenças extra intestinais graves caracterizadas por anemia hemolítica microangiopática, trombocitopenia e insuficiência renal aguda. Além da produção de shigatoxinas, a expressão de intimina e a produção de enterohemolisinas são atributos de virulência também descritos no grupo patogênico. Ruminantes, especialmente bovinos, são reconhecidos como o principal reservatório das STEC e o manejo inadequado desses animais pode representar um risco de contágio e de disseminação desses patógenos. O estudo incluiu 301 isolados de E. coli obtidos de amostras fecais de bovinos, com e sem diarreia, de regiões agropecuárias brasileiras no Rio de Janeiro e em Rondônia. As amostras bacterianas foram caracterizadas quanto ao polimorfismo genético e a presença de marcadores genéticos codificadores de virulência e dos grupos antigênicos associados ao patotipo. Ensaios moleculares revelaram que 55,5% (167/301) das amostras de E. coli foram carreadoras do gene stx, 36,2% (109/301) e 21,3% (64/301) dos genes eae+ e ehxA+, respectivamente. Dentre as STEC, 24 foram isoladas do Rio de Janeiro (14,4%, 24/167) e 143 de Rondônia (85,6%, 143/167). 13 genótipos de virulência foram observados: stx1 /stx2 /eae/ehxA, stx1 /stx2 /eae, stx1 /stx2 /ehxA, stx1 /stx2 , stx1 / eae/ehxA, stx1 /ehxA, stx1 , stx2 /eae/ehxA, stx2 /eae, stx2 /ehxA, stx2 , eae/ehxA e ehxA). 3,6% (6/167) e 93,4% (156/167) das STEC foram carreadoras dos genes rfbO113 e rfbO157+, respectivamente, sendo 64,1% desses rfbO157+h7+ (100/156). A tipagem da região LEE revelou os subtipos eaeα (3/109), eaeβ (6/109), eaeγ (4/109), tirα (2/109), tirβ (3/109), tirγ (3/109), espAα (3/109), espAβ (0/109), espAγ (0/109), espBα (1/109), espBβ (9/109) e espBγ (0/109). A filogrupagem classificou as STEC nos grupos A (44,9%), B1 (47,3%), D (6,6%) e B2 (1,2%). A tipagem pelo RAPD- revelou uma ampla diversidade genética entre as STEC indicando constituir uma população bacteriana de origem não clonal. A elevada prevalência de STEC em bovinos clinicamente sadios confirma o seu papel como importantes reservatórios ambientais. A ampla circulação desses patógenos em animais de propriedades rurais, em especial, aquelas cujas condições de infraestrutura e boas práticas de manejo são insatisfatórias, representa um sério risco ambiental de concentração desses microrganismos, propiciando a exposição e a ocorrência de doenças e das demais manifestações clínicas relacionadas ao patógeno. O estudo é dividido em duas partes, sendo que o primeiro capítulo apresenta os aspectos introdutórios e metodológicos e por meio do segundo são mostrados os resultados, discussão e conclusões.
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da Costa, Cristiane Mara Silva, Adriana Hamond Regua Mangia, Alice Gonçalves Martins Gonzalez, Bruno Gomes de Castro, and Denis Yukio Otaka. "CARACTERIZAÇÃO MOLECULAR DE AMOSTRAS DE ESCHERICHIA COLI CARREADORAS DOS GENES STX ISOLADAS DE BOVINOS DE REGIÕES AGROPECUÁRIAS BRASILEIRAS." In Estudos Avançados em Conservação e Biodiversidade. Bookerfield Editora, 2022. http://dx.doi.org/10.53268/bkf22080401.
Full textAbstract:
Escherichia coli produtora da toxina Shiga (STEC) é um microrganismo agente de infecções de amplo espectro clínico incluindo desde quadros assintomáticos, casos diarreicos leves até doenças extra intestinais graves caracterizadas por anemia hemolítica microangiopática, trombocitopenia e insuficiência renal aguda. Além da produção de toxina Shiga, a expressão de intimina e a produção de enterohemolisinas são atributos de virulência também descritos no grupo patogênico. Ruminantes, especialmente bovinos, são reconhecidos como o principal reservatório das STEC e o manejo inadequado desses animais pode representar um risco de contágio e de disseminação desses patógenos. O estudo incluiu 301 isolados de E. coli obtidos de amostras fecais de bovinos, com e sem diarreia, de regiões agropecuárias brasileiras no Rio de Janeiro e em Rondônia. As amostras bacterianas foram caracterizadas quanto ao polimorfismo genético e a presença de marcadores genéticos codificadores de virulência e dos grupos antigênicos associados ao CARACTERIZAÇÃO MOLECULAR DE AMOSTRAS DE escherichia coli CARREADORAS DOS GENES STX ISOLADAS DE BOVINOS DE REGIÕES AGROPECUÁRIAS BRASILEIRAS Capítulo 1 14 CARACTERIZAÇÃO MOLECULAR DE AMOSTRAS DE Escherichia coli CARREADORAS DOS GENES STX ISOLADAS DE BOVINOS DE REGIÕES AGROPECUÁRIAS BRASILEIRAS patotipo. Ensaios moleculares revelaram que 55,5% (167/301) das amostras de E. coli foram carreadoras do gene stx, 36,2% (109/301) e 21,3% (64/301) dos genes eae+ e ehxA+, respectivamente. Dentre as STEC, 24 foram isoladas do Rio de Janeiro (14,4%, 24/167) e 143 de Rondônia (85,6%, 143/167). Treze genótipos de virulência foram observados: stx1 /stx2 /eae/ehxA, stx1 /stx2 / eae, stx1 /stx2 /ehxA, stx1 /stx2 , stx1 /eae/ehxA, stx1 /ehxA, stx1 , stx2 /eae/ehxA, stx2 /eae, stx2 /ehxA, stx2 , eae/ehxA e ehxA). 3,6% (6/167) e 93,4% (156/167) das STEC foram carreadoras dos genes rfbO113 e rfbO157+, respectivamente, sendo 64,1% desses rfbO157+h7+ (100/156). A tipagem da região LEE revelou os subtipos eaeα (3/109), eaeβ (6/109), eaeγ (4/109), tirα (2/109), tirβ (3/109), tirγ (3/109), espAα (3/109), espAβ (0/109), espAγ (0/109), espBα (1/109), espBβ (9/109) e espBγ (0/109). A filogrupagem classificou as STEC nos grupos A (44,9%), B1 (47,3%), D (6,6%) e B2 (1,2%). A tipagem pelo RAPD revelou uma ampla diversidade genética entre as STEC indicando constituir uma população bacteriana de origem não clonal. A elevada prevalência de STEC em bovinos clinicamente sadios confirma o seu papel como importantes reservatórios ambientais. A ampla circulação desses patógenos em animais de propriedades rurais, em especial, aquelas cujas condições de infraestrutura e boas práticas de manejo são insatisfatórias, representa um sério risco ambiental de concentração desses microrganismos, propiciando a exposição e a ocorrência de doenças e das demais manifestações clínicas relacionadas ao patógeno.
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Matthews, Karl R. "Advances in understanding the contamination of fresh produce by pathogenic Escherichia coli." In Advances in ensuring the microbiological safety of fresh produce. Burleigh Dodds Science Publishing, 2023. http://dx.doi.org/10.19103/as.2023.0121.03.
Full textAbstract:
Produce is a constituent of healthy diets throughout the world. Contamination of produce with pathogenic Escherichia coli and particularly Shiga toxin producing E. coli (STEC) is a global human health concern. STEC may enter fields in which produce is grown through contaminated irrigation water, wild and domestic animals, soil inputs, run-off, workers, and equipment. The knowledge of ability of STEC to persist and survive in the environment (soil, water, etc.) will facilitate innovation of systems that will enhance microbial safety of produce. Researchers are beginning to unravel the complex interaction of STEC with plants intended for human consumption. In this chapter focus is placed on E. coli contamination of produce addressing trends in occurrence in types of fresh produce; developments in understanding genetics, behavior and virulence; new research on growth and survival in soil and plants; and implications for control.
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Sajid Abdulabbas, Hadi, Noor Al-Khafaji, Suhad Y. Abed, Hussein Al-Dahmoshi, and Huda Najh Al-Baroody. "Phylotypes and Pathotypes of Diarrheagenic Escherichia coli of Gastroenteritis." In Escherichia coli Infections - An Update [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.109860.
Full textAbstract:
Escherichia coli responsible for wide range of common bacterial infections, the frequent one is gastroenteritis. Bacterial gastroenteritis mainly attributed to diarrheagenic E. coli and accompanied by diarrhea and vomiting. Actually pathogenic E. coli can be classified according to the site of infection whether it be within intestine (called intestinal pathogenic E. coli InPEC) or cause infection outside intestine (called extraintestinal pathogenic E. coli ExPEC). They are assigned to 4 main phylogenetic groups: InPEC include A and B1 while ExPEC have B2 and D groups. Seven Pathotypes have been assigned: Enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohemorrhagic (Shiga-toxin producing E. coli (EHEC/STEC), enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC), enteroinvasive E. coli (EIEC) and adherent invasive E. coli (AIEC). The patho-phylotyping of diarrheagenic E. coli interaction along with antibiotic resistance and biofilm formation capacity may be valuable insight to know real threat of this pathogen and this is tried to be covered with this chapter. The results revealed that the among DEC, EPEC and ETEC were assigned in high rate to B1 followed by A, B2, D, E, C and F while EAEC show different assignment: D followed by B2, A, B1, C, E and F. The other DEC pathotypes showed different styles.
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"AOAC Official Method 2017.05 Escherichia coli O157:H7 and E. coli non-O157 Shiga Toxin-Producing E. coli (STEC) in Select Foods." In Official Methods of Analysis of AOAC INTERNATIONAL, 22nd ed. Oxford University Press, 2023. http://dx.doi.org/10.1093/9780197610145.003.2216.
Full textConference papers on the topic "E-Steco"
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Gomes, Marcel da Silva Amorim, Raquel Nogueira de Medeiros, and Alice Gonçalves Martins Gonzalez. "Comportamento de Escherichia Coli Produtora de Toxina Shiga (Stec) Sob O Efeito de Diferentes Níveis de Temperatura e Ph." In XII Latin American Congress on Food Microbiology and Hygiene. Editora Edgard Blücher, 2014. http://dx.doi.org/10.5151/foodsci-microal-042.
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