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1

Suwanruji, Potjanart, and Harold S. Freeman. "Design, synthesis and application of easy wash-off reactive dyes." Coloration Technology 122, no. 1 (February 2006): 27–36. http://dx.doi.org/10.1111/j.1478-4408.2006.00002.x.

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2

Nagata, R. T. "Clip-and-wash Method of Emasculation for Lettuce." HortScience 27, no. 8 (August 1992): 907–8. http://dx.doi.org/10.21273/hortsci.27.8.907.

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Complete (100%) hybridization in lettuce (Lactuca sativa L.) was accomplished consistently using the clip-and-wash method of emasculation. The clip-and-wash method is a combination of washing and clipping, two previously described procedures for pollen removal. The wash and the clip methods produced 98% and 95% hybridization, respectively. The method is quick and easy and eliminates inadvertent self pollination; with it one can produce the many hybrid seeds necessary for backcross and F1 genetic analysis in lettuce.
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3

OHASHI, Kenji, and Takashi MENO. "The Carbon Footprint and the Water Consumption of Easy-Rinse Foaming Face Wash." Journal of Life Cycle Assessment, Japan 14, no. 2 (2018): 161–70. http://dx.doi.org/10.3370/lca.14.161.

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4

Ong, Geraldine D., Mehrnoush Naim, Qi Wang, Rolando Gumatay, Sharon Daroy-Adriatico, and Xiaohai Zhang. "P088 Laminar wash systems – Cost effective and easy automation of flow cytometry assays." Human Immunology 80 (September 2019): 118. http://dx.doi.org/10.1016/j.humimm.2019.07.141.

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5

Simonne, Eric, Harry A. Mills, and Doyle A. Smittle. "A Simple Method to Measure On-farm Pan Evaporation for Scheduling Irrigation." HortTechnology 2, no. 3 (July 1992): 392–93. http://dx.doi.org/10.21273/horttech.2.3.392.

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Measurements of daily, 3-day, and 6-day cumulative pan evaporation using a #2 wash tub or a modified steel drum and a ruler provided an accurate, easy, and inexpensive way to schedule irrigation. Pan factors for these containers, which were covered with a 5-cm-mesh wire under humid climatic conditions, were 1.0 and 1.1, respectively.
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6

Kenji, OHASHI, and MENO Takashi. "Erratum to “The Carbon Footprint and the Water Consumption of Easy-Rinse Foaming Face Wash”." Journal of Life Cycle Assessment, Japan 15, no. 4 (2019): 377. http://dx.doi.org/10.3370/lca.15.377.

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7

Noyola, Daniel E., Bruce Clark, Frederick T. O'Donnell, Robert L. Atmar, Jewel Greer, and Gail J. Demmler. "Comparison of a New Neuraminidase Detection Assay with an Enzyme Immunoassay, Immunofluorescence, and Culture for Rapid Detection of Influenza A and B Viruses in Nasal Wash Specimens." Journal of Clinical Microbiology 38, no. 3 (2000): 1161–65. http://dx.doi.org/10.1128/jcm.38.3.1161-1165.2000.

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The performance of a new, rapid, easy-to-perform assay based on neuraminidase enzyme activity for detection of influenza virus types A and B was compared to detection by culture, indirect immunofluorescence, and enzyme immunoassay in 479 nasal wash specimens from children with respiratory infections. Compared to isolation of influenza virus by culture, the neuraminidase assay had a sensitivity of 70.1%, specificity of 92.4%, positive predictive value of 76.3%, and negative predictive value of 89.9%. There was a higher sensitivity for the detection of influenza A virus (76.4%) than for influenza B virus (40.9%). Indirect immunofluorescence showed a sensitivity of 59.8% and specificity of 97% compared to culture isolation for detection of influenza A and B viruses. Enzyme immunoassay showed a sensitivity of 89.7% and specificity of 98.1% for the detection of influenza A alone. The quality of the nasal wash specimen had a significant effect on the detection of influenza virus by all of the assays. A strong response of the neuraminidase assay was more likely to represent a culture-confirmed influenza infection. This new rapid neuraminidase assay was useful for the detection of influenza A and B viruses in nasal wash specimens.
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8

Lamberts*, Mary, Eugene McAvoy, Teresa Olczyk, and Phyllis Gilreath. "Simple Teaching Tools for Verifying Hand Washing Skills." HortScience 39, no. 4 (July 2004): 843C—843. http://dx.doi.org/10.21273/hortsci.39.4.843c.

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U.S. agricultural producers are required to provide varying amounts of safety training to their employees depending on the nature of their operation(s). Hand washing is an integral part of several types of safety training including pesticide safety education, the Worker Protection Standard and Microbial Food Safety of Fruits and Vegetables. Generally instructions are to “wash thoroughly,” though some employees are told they should wash for 20 seconds. An easy way to get growers to “buy into” methods that verify hand washing is to include such demonstrations as part of pesticide safety education programs and workshops that grant Continuing Education Units (CEUs) for the renewal of pesticide applicator licenses. It is important that the demonstrations be highly visual so participants actually experience the difficulty in removing a contaminant from hands even though they have performed “thorough” hand washing. It also allows them to observe the ease of cross contamination from soiled hands. Once growers see how easy and inexpensive it is to do this type of training, they are being encouraged to use these demonstrations with various types of employees: mixer-loaders and other handlers, harvesting crews, packinghouse employees, and even field workers who routinely handle plants and may be spreading diseases. Details on different methods of training and grower reactions will be presented.
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9

Helweg-Larsen, Jannik, Jørgen Skov Jensen, Thomas Benfield, Ulrik Gerner Svendsen, Jens D. Lundgren, and Bettina Lundgren. "Diagnostic Use of PCR for Detection ofPneumocystis carinii in Oral Wash Samples." Journal of Clinical Microbiology 36, no. 7 (1998): 2068–72. http://dx.doi.org/10.1128/jcm.36.7.2068-2072.1998.

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There is a need to develop noninvasive methods for the diagnosis ofPneumocystis carinii pneumonia in patients unable to undergo bronchoscopy or induction sputum. Oral wash specimens are easily obtained, and P. ca- rinii nucleic acid can be amplified and demonstrated by PCR. In routine clinical use, easy sample processing and single-round PCR are needed to ensure rapid analysis and to reduce the risk of contamination. We developed a single-round Touchdown PCR (TD-PCR) protocol with the ability to detect PCR inhibition in the specimen. The TD-PCR was evaluated in a routine diagnostic laboratory and was compared to a previously described PCR protocol (mitochondrial RNA) run in a research laboratory. Both PCR methods amplified a sequence of the mitochondrial rRNA gene of P. carinii. Paired bronchoalveolar lavage (BAL) and oral wash specimens from 76 consecutive human immunodeficiency virus type 1-infected persons undergoing a diagnostic bronchoscopy were included. The TD-PCR procedure was quicker than the mitochondrial PCR procedure (<24 versus 48 h) and, compared to microscopy, had sensitivity, specificity, and positive and negative predictive values of 89, 94, 93, and 91%, respectively, for oral wash specimens and 100, 91, 90, and 100%, respectively, for BAL specimens. Our results suggest that oral wash specimens are a potential noninvasive method to obtain a diagnostic specimen duringP. carinii pneumonia infection and that it can be applied in a routine diagnostic laboratory.
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10

Kushida, Tsuyuka, Haruyuki Iijima, Hiroshi Kushida, and Chusei Tsuruta. "En bloc staining available for stereoscopic observation of epoxy resin Quetol 651-embedded thick sections under a 300 kV TEM." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (August 12, 1990): 740–41. http://dx.doi.org/10.1017/s0424820100161266.

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A staining method has been devised for easy en bloc staining for stereoscopic observation of epoxy resin Quetol 651-embedded thick sections under a 300 kV transmission microscope (TEM). In order to enhance staining properties in thick section, osmium tetroxide-fixed tissue blocks are stained only en bloc, since the images of both sides in thick section give high contrast and the image of an intermediate layer shows low contrast by double staining.This method uses carbohydrazide (Polysciences, Inc., U.S.A.) as osmium bridging agent, and both osmium tetroxide and uranyl acetate as electron staining agents.The following procedure is suitable for en bloc staining. 1.Fix small tissue blocks in 2% cacodylate-buffered osmium tetroxide (pH 7.4) for 3 hours at 4°C.2.Wash well in buffer for 1 hour.3.Transfer in 1% aqueous carbohydrazide for 2 hours at room temperature.4.Wash well in distilled water for 1 hour.5.Stain in 1% aqueous osmium tetroxide for 2 hours at room temperature.6.Wash well in distilled water for 1 hour.7.Dehydrate in 50% alcohol for 1 hour.8.Stain in a 2.5% solution of uranyl acetate in 50% alcohol for 3 hours at room temperature.9.Wash in 50% alcohol for 1 hour.10.Dehydrate with 60%, 70%, 80%, 90% and 100% (2 changes) alcohols for 30 minutes each.11.Embed in a mixture of Quetol 651 (Nissin EM Co., Ltd., Japan), nonenyl succinic anhydride, methyl nadic anhydride and DMP-30 according to the method of Kushida et al.
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11

Bhandari, Babita, and Anita Rani. "Standardization and utilization of Himalayan nettle (Girardinia diversifolia) roots for the dyeing of silk fabric." Environment Conservation Journal 22, no. 1&2 (June 4, 2021): 19–25. http://dx.doi.org/10.36953/ecj.2021.221204.

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The nature is abode to numerous plants, animal and mineral resources that offersustainable alternative to chemicalsused in textile dyeing. In this paper an effort has been made to utilize the roots of Himalayan nettle plant for fabric dyeing which grows abundantly in-the higher altitude of Uttarakhand as a weed plant. The extraction and dyeing variables were optimized for application on silk fabric on the basis of colour strength and wash fastness rating. Dye extraction carried out in aqueous medium at simmering temperature for 1.5 hoursresulted in better colour depth and washing fastness. Similarly, dyeing of silk at 90ºC for 90 minutes using 3 gm per 100 ml powdered dye material yielded better dyed samples in terms of colour strength and wash fastness. The obtained dye recipe is easy to be used by dyeing units at minimal cost since no chemicals are added during dyeing and raw material can be collected from pastures, around croplands and nearby forest areas.
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12

Huang, J. Y., T. Uchiburi, and K. Fujita. "Membrane filtration retentate thickening efficiency using electrolytic ion products instead of chemical coagulants." Water Supply 3, no. 3 (June 1, 2003): 43–48. http://dx.doi.org/10.2166/ws.2003.0006.

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Electrolytic coagulation has advantages compared with conventional chemicals coagulation treatment. First of all, the addition of alkaline agent is necessary in chemicals coagulation treatment as the addition of acid agents in coagulation of dense wash water makes pH decline. While not much change of pH is observed with electrolytic coagulation, where alkaline agent is not demanded. Secondly, it is easy to control the dose of additional coagulants. In this study, electrolytic coagulation sedimentation treatment is adopted for membrane retentate from membrane filtration treatment without using coagulants. As electricity can control the addition of coagulation, an automation method is applied in the experiment. The lower the iron as well as aluminum dissolved, the higher the sludge density becomes; especially aluminum has this tendency. It is possible to achieve 400 kg/m3 of condensed sludge density with artificial wash water and more than 80 kg/m3 for real retentate, in terms of electrolytic coagulation of iron anode. The sludge density of aluminum anode is higher, but its filtrate density is lower than iron anode.
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13

Besley, M., A. Thomas, S. Salter, Y. Y. Sang, M. Vas, and P. R. Uldall. "Control of Oral Anticoagulation in Patients Using Long-Term Internal Jugular Catheters for Haemodialysis Access." International Journal of Artificial Organs 15, no. 5 (May 1992): 277–80. http://dx.doi.org/10.1177/039139889201500504.

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Patients using long-term double-lumen silastic jugular catheters for haemodialysis access frequently require oral anticoagulants to maintain patency of the catheter. It may be difficult or impossible to obtain peripheral vein blood samples for PT measurements to regulate the oral anticoagulant dose. Our studies have shown that removal of 3 mis of blood from the catheter limb containing the heparin (to be discarded) followed by 10 mis as a heparin wash-out (to be returned to the patient) allows blood to be taken from the arterial limb of the catheter for PT measurement. The PT measurement obtained from the arterial limb after a 10 ml wash-out is nearly always identical with the PT measurement on blood obtained from a peripheral vein. If the simultaneously measured aPTT is normal the PT is predictably and consistently accurate. If the aPTT is raised due to traces of residual heparin then the PT may be inaccurate and should be repeated. This method is easy and reliable and can be recommended as an aid to regulation of the oral anticoagulant dose
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14

Tu, Ji Li, Chun Jie Qian, Hua Yun Ge, Ji Ping Wang, and Jin Qiang Liu. "The Impact of Liquid Surface Tension on Fabric Drying Efficiency." Advanced Materials Research 441 (January 2012): 613–18. http://dx.doi.org/10.4028/www.scientific.net/amr.441.613.

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This study presents an experimental investigation of the relationship between liquid surface tension and fabrics water retention in dehydration processes such as centrifuging, line drying and heat drying. Selected surfactants were used to prepare wash baths with different surface tension, and dehydrating experiments of cotton fabric after immersion in above bath were conducted. The results showed that lower surface tension is beneficial to reducing fabrics water retention by centrifuging and improving line drying efficiency and heat drying efficiency. It was assumed that water with low surface tension is easy to drop down or separate from fabric, thus improving the de-watering and drying efficiency.
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15

Makabe, Sachiko, Katsushi Maeda, Sayaka Izumori, Emiko Konno, Yayoi Sato, Nana Yoshioka, Hideko Shirakawa, and Kenji Ando. "Developing Disposable Hair Washing Pad for Bedridden Patients Using Mixed Methods Research." Global Journal of Health Science 11, no. 6 (April 29, 2019): 70. http://dx.doi.org/10.5539/gjhs.v11n6p70.

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In Japan, diapers are often used to wash bedridden patients&rsquo; hair by placing the diaper under the patient&rsquo;s head for ease of use and efficiency. However, using diapers for the head is an ethical problem as diapers are originally used for elimination care. Developing better equipment to wash hair is necessary to comfort patients and reduce nurses&rsquo; workload. Our industry-academia-clinical collaboration team developed a disposable absorbent pad particularly for washing patients&rsquo; hair in bed. This study aimed to evaluate this pad developed for bedridden patients. The trial was conducted in five departments at a university hospital between May and August 2016. The post-trial cross-sectional survey for nurses contained demographic data, evaluation of the pad with rating score as quantitative data, free comments as qualitative data, and comparison with diaper used experience. As this study involved development of an equipment and thus to facilitate data triangulation, mixed methods were used. The results revealed that 36 nurses participated (90% response rate). Most were in their 20s (69%). The overall evaluation was &ldquo;good&rdquo; (91%). Good water absorption, no water leakage, and easy usage were reported. In a comparison with diapers using experience, the majority preferred the developed pad (81%). Better structure, usage, and resolution of ethical issues were also confirmed in a comparison with diapers. The developed disposable hair washing pad is an efficient tool for nurses to wash bedridden patients&rsquo; hair. As it is disposable, infections are also controlled well. Further manufacturing aspects need to be considered for mega production.
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16

Iwamoto, Chihiro, Ai Oyama, Takayuki Kumei, and Junji Watanabe. "Development of a Powder with Water-Resistance and Easy-Wash-Off Properties Using Poly (Trimethylene Carbonate) Derivatives and Its Application to Cosmetics." Journal of Society of Cosmetic Chemists of Japan 52, no. 3 (September 20, 2018): 197–204. http://dx.doi.org/10.5107/sccj.52.197.

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17

Md Saleh, Noorashikin. "Vortex Assisted Extraction for Propyl Paraben Analysis in Cosmetics." ASM Science Journal 12 (November 5, 2019): 1–8. http://dx.doi.org/10.32802/asmscj.2019.310.

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Propylparaben is an ester of para-hydroxybenzoic acid and commonly used as a preservative in the cosmetics, pharmaceuticals and food products. However, several recent reports demonstrated that paraben possesses an estrogenic activity and causes cancer for the consumer. Therefore, the extraction of propylparaben from cosmetics requires method that is easy, fast, user-friendly and accurate reproducible result. Conventional techniques such as liquid-liquid extraction (LLE) show some disadvantages such as the use of a large amount of solvent and time-consuming. Therefore, this present study aims to apply vortex assisted extraction (VAE) in determining the concentration of propylparaben in cosmetic samples and analyse using ultraviolet spectroscopy (UV-Vis). From the results, the calibration curve was found in the range of 0.2-1.0mg/L with a regression coefficient, r2=0.9932 and relative standard deviation (RSD) less than 1%. The limit of detection (LOD) and quantification (LOQ) for VAE was 0.090mg/L and 0.302mg/L, respectively. Operating parameters for VAE (concentration of salt, type of solvent and extraction time) and LLE (concentration of salt, extraction time, type and volume of solvent) have been optimised and subsequently applied to the extraction using the real samples. Ten cosmetic products were chosen randomly such as shampoo, body wash, gargle, toner, mouth rinse, lotion, feminine wash, face mask, and scrub. The recoveries for VAE and LLE were 76.7% -103.4% (RSD=0.1-4.0%) and 62.5%-93.9% (RSD=<1%), respectively. Therefore, VAE is the best modern method for determination of propylparaben in cosmetics because it is simpler, faster with high percentage of recovery compared to other techniques.
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18

Paramawidhita, Risqika Yuliatantri, Uswatun Chasanah, and Dian Ermawati. "Formulasi dan Evaluasi Fisik Sediaan Emulgel Tabir Surya Ekstrak Kulit Batang Kayu Manis (Cinnamomum burmannii)." Jurnal Surya Medika 5, no. 1 (August 31, 2019): 90–99. http://dx.doi.org/10.33084/jsm.v5i1.950.

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One of the natural ingredients can be used for sunscreen is Cinnamomum burmannii. Sinamaldehid chemical constituents in cinnamon bark extract that has potential as an antioxidant and can be used as a sunscreen content. This study was to determine the levels of cinnamon bark extract ( 1 %, 2 % and 3 % ) which can give physical characteristics, acceptability, which is optimal for preparations with HPMC as a gelling agent, Evaluations include pH, the power spread, the viscosity and acceptability. The results of organoleptic for formula I (1%), II (2%) and III (3%) have a soft texture grainy, the distinctive smell of cinnamon, and all formula has a pale brown color. From the analysis of One-Way ANOVA found significant differences for any dispersive power. But did not have significant differences in pH and viscosity. For acceptability evaluation formula III is the optimal formula to applied softness, easiest formula to flattened and easy to wash.
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19

Peng, Mengna, Can Liu, Shaojuan Chen, Songwei Gao, Liang Jiang, and Jianwei Ma. "Development and performance study of a new shrink-proof and non-iron cotton blended fabric." Textile Research Journal 89, no. 16 (November 4, 2018): 3269–79. http://dx.doi.org/10.1177/0040517518809043.

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The approaches for cotton shrink-resistant finishing can be categorized into chemical finishing and physical finishing. In this research, a new approach to prevent cotton fabric from shrinkage and wrinkling without resin finishing was studied to reach an easy care effect of “wash-and-wear” for related end products. It was achieved by adding a certain proportion of fine denier polyester and memory polyester fibers. Unlike the previous chemical finishing methods, this physical finishing method does not cause yellowing, formaldehyde content increase or strong damage of tensile strength. It can reduce the shrinkage rate and increases the wrinkle recovery angle (WRA) through the shape memory formed by the memory polyester fiber in the heat setting stage. Our results were of better condition with significant improvements in WRA, anti-pilling effect, shrink-proofing and strength of the fabrics. The fabrics can be widely used in bedding textiles and shirting fabrics.
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20

Brunton, Elizabeth R., Mark N. Burgess, Ian P. Whelan, and Ian F. Burgess. "A Cosmetically Acceptable Dye Product to Improve Detection of Head Louse Eggs and Nits." Cosmetics 7, no. 1 (March 24, 2020): 19. http://dx.doi.org/10.3390/cosmetics7010019.

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Removing head louse eggshells and nits after a successful pediculicide treatment is often made more difficult because it is not easy to see them all amongst the hairs. Therefore, a treatment that makes louse eggs more visible potentially makes the task of removal easier and reduces the risk that children would be sent home from school or over-treated. This project involved the development of a hair treatment gel concept that was easy to apply, wash out, and that stained louse eggs and nits but without staining the hair and skin. A series of food-grade dyes were evaluated for their ability to stain the eggshells, and various copolymer-based rheology modifiers were tested for dye compatibility and stability. Several dyes were rejected because either they failed to stain louse eggshells or else stained skin too easily. Two dyes, Patent Blue and Ponceau 4R, were eventually selected for different product developments, one specifically for post-treatment nit removal and the other for pre-treatment diagnosis. In clinical field studies, both were found to make the treated eggshells contrast sufficiently with the hair to enable identification of persistent nits. Use of a nit stain product can enable easier detection of louse eggs and nits, thus facilitating the removal process and reducing the risk that persistent eggshells could be mistaken for signs of a continuing infestation.
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21

Istiqomah, Nurul, and Juliyanti Akuba. "FORMULASI EMULGEL DARI EKSTRAK DAUN KELOR (Moringa oleifera LAM) SERTA EVALUASI AKTIVITAS ANTIOKSIDAN DENGAN METODE DPPH." Journal Syifa Sciences and Clinical Research 3, no. 1 (March 3, 2021): 9–18. http://dx.doi.org/10.37311/jsscr.v3i1.9874.

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Moringa (Moringa oleifera Lam) is one of the plants that has high antioxidant activity, especially in the leaves. Emulgel is one of the topical dosage which dermatologically has several beneficial properties, namely thixotropic, not oily, easy to spread, easy to clean, soft, easy to wash, long lasting, transparent and comfortable when used. The purpose of this research was to formulate moringa (Moringa oleifera Lam) leaves extract into emulgel dosage forms and determine the antioxidant activity of the dosage using DPPH method.The research began with extraction of moringa leaves and optimization of the base by varying the concentration of carbopol 940 as gelling consisting of F1 0.5%, F2 1%, F3 1.5% and F4 2%. The base that met the requirements of good physical stability was F2. The F2 base was then made into emulgel dosage with 3 concentration variations of the extract, namely F2a 4%, F2b 5% and F2c 6%. The physical stability test result of each formula met the organoleptic test,the pH test, the dispersion test, the adhesion test, the viscosity test, and the freeze-thaw test. The One way ANOVA statistical test result showed that the p value was greater than 0.05, which meant that the emulgel dosage had good physical stability. The IC50values of each antioxidant activity result were F2a (t0 = 120.464 g/mL; t28 = 144.887 g/mL), F2b (t0 = 113.642 g/mL; t28 = 128.407g/mL), F2c (t0 = 74.745 g/mL; t28 = 90.618 g/mL). The statistical results of the t-test showed thep value = 0,027, (0.05), This indicated that there were significant difference results of the antioxidant activity test between the three formulas on the first day (t0) and on the 28th day (t28).
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22

Rauf, Jais, Ishak Isa, and Nur Ain Thomas. "Formulasi Sediaan Emulgel Ekstrak Daun Kelor (Moringa oleifera Lam) Dan Uji Aktivitas Antioksidan Menggunakan Metode DPPh." Indonesian Journal of Pharmaceutical Education 1, no. 1 (February 14, 2021): 10–19. http://dx.doi.org/10.37311/ijpe.v1i1.9947.

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Moringa (Moringa oleifera Lam) is one of the plants that has high antioxidant activity, especially in the leaves. Emulgel is one of the topical dosage which dermatologically has several beneficial properties, namely thixotropic, not oily, easy to spread, easy to clean, soft, easy to wash, long lasting, transparent and comfortable when used. The purpose of this research was to formulate moringa (Moringa oleifera Lam) leaves extract into emulgel dosage forms and determine the antioxidant activity of the dosage using DPPH method.The research began with extraction of moringa leaves and optimization of the base by varying the concentration of carbopol 940 as gelling consisting of F1 0.5%, F2 1%, F3 1.5% and F4 2%. The base that met the requirements of good physical stability was F2. The F2 base was then made into emulgel dosage with 3 concentration variations of the extract, namely F2a 4%, F2b 5% and F2c 6%. The physical stability test result of each formula met the organoleptic test,the pH test, the dispersion test, the adhesion test, the viscosity test, and the freeze-thaw test. The One way ANOVA statistical test result showed that the p value was greater than 0.05, which meant that the emulgel dosage had good physical stability. The IC50values of each antioxidant activity result were F2a (t0 = 120.464 g/mL; t28 = 144.887 g/mL), F2b (t0 = 113.642 g/mL; t28 = 128.407g/mL), F2c (t0 = 74.745 g/mL; t28 = 90.618 g/mL). The statistical results of the t-test showed thep value = 0,027, (0.05), This indicated that there were significant difference results of the antioxidant activity test between the three formulas on the first day (t0) and on the 28th day (t28).
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23

Suematsu, Naoya, and Fumihide Isohashi. "Rapid and simple screening of transgenic mice: novel extraction-free, filter-based PCR genotyping from blood samples." Acta Biochimica Polonica 53, no. 3 (September 2, 2006): 613–16. http://dx.doi.org/10.18388/abp.2006_3336.

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We evaluated the effectiveness of using Flinders Technology Associates (FTA) filter paper for the polymerase chain reaction (PCR) genotyping of transgenic mice. Tail prick blood sample dried on an FTA filter disc was processed for genomic PCR. It is easy and rapid to prepare DNA templates because the protocol is extraction-free and only requires minimal handling of wash briefly bloodstained FTA filter discs. Progeny of a transgene-positive founder mated with wild-type mice was screened for the presence of the transgene by the filter-based PCR using transgene-specific primers. The resulting amplicons with expected sizes of 3134 bp, 1152 bp, 877 bp and 688 bp were robust and reproducible, allowing a distinction between transgenic (n=44) and wild-type (n=47) mice showing no signal. The filter-based PCR screening took only half a day. The present study confirmed the validity and usefulness of the novel rapid extraction-free genotyping method.
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24

Koreman, Megan. "The Collaborator's Penance: The Local Purge, 1944–5." Contemporary European History 6, no. 2 (July 1997): 177–92. http://dx.doi.org/10.1017/s0960777300004513.

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Despite the neat treaties of diplomats and precise agreements of generals, wars like the Second World War end untidily. Amidst the ruins of cities and the wash of refugees, the cessation of international hostilities gives way to the settling of domestic accounts, which in 1944–5 was called the purge of collaborators. As common and as compelling as the purge was, historians have paid relatively little attention to it. The French purge, for instance, remains somewhat of an historical enigma, in part because of the archival laws that shield the judicial records, but also in part because of the apparently obvious nature of the purge. Who cannot sympathise, even if reluctantly, with the bereaved survivors who took vengeance on the collaborator who murdered their husbands or wives, fathers, daughters or beloved friends? But such easy sympathy has misled scholarly analysis into dividing the French purge into a legal purge of court action and a popular purge of vigilante violence.
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25

Reinap, A., B. L. B. Wiman, S. Gunnarsson, and B. Svenningsson. "Dry deposition of NaCl aerosols: theory and method for a modified leaf-washing technique." Atmospheric Measurement Techniques Discussions 3, no. 4 (August 25, 2010): 3851–76. http://dx.doi.org/10.5194/amtd-3-3851-2010.

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Abstract. Within the framework of aerosol deposition to vegetation we present a specially designed leaf wash-off method used in a wind-tunnel based study, where leaves of Quercus robur L. were exposed to NaCl aerosols. We summarise the principles and illustrate the method for two types of substances, the chloride ion and the sodium ion, and for two levels of aerosol exposure prior to leaf washing. On the average, in the low-exposure experiments (S1), the 1st (2nd) wash-off step provided 90% (96%) of the amount of Cl− on the leaves. In the high-exposure experiments (S2) the corresponding values were 96% and 99%. For sodium, the general dynamics resembles that of chloride, but the amounts washed off were, in both series, on the average below what would be expected if the equivalent ratio in the tunnel aerosol were to be preserved. Na+ showed adsorption and/or absorption at the leaf surfaces. The difference between the mean values of the amounts of chloride and of sodium washed off in S1 was not statistically significant, the mean Na+ to Cl− difference as a fraction of Cl− being minus 18%±27%; corresponding values for S2 were minus 16%±9%, however (p<0.05). In the latter case, 101±57 μequiv Na+ per m2 of leaf area were missing for the equivalent relationship 1:1 with Cl− to be met. Although uncertainties are thus large, this indicates the magnitude of the Na+-retention. The method is suitable not only for chloride, an inexpensive and easy-to-handle tracer, but also for sodium under exposure at high aerosol concentrations. Our findings will help design further studies of aerosol/forest interactions.
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Jagadeesan, Kishore Kumar, and Simon Ekström. "MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis." SLAS DISCOVERY: Advancing the Science of Drug Discovery 22, no. 10 (August 21, 2017): 1246–52. http://dx.doi.org/10.1177/2472555217727517.

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Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.
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YOUNG, IAN, ANDRIJANA RAJIĆ, ANN LETELLIER, BILL COX, MIRA LESLIE, BABAK SANEI, and SCOTT A. McEWEN. "Knowledge and Attitudes toward Food Safety and Use of Good Production Practices among Canadian Broiler Chicken Producers." Journal of Food Protection 73, no. 7 (July 1, 2010): 1278–87. http://dx.doi.org/10.4315/0362-028x-73.7.1278.

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Provincial broiler-chicken marketing boards in Canada have recently implemented an on-farm food safety program called Safe, Safer, Safest. The purpose of this study was to measure broiler chicken producers' attitudes toward the program and food safety topics and use of highly recommended good production practices (GPP). Mailed and Web-based questionnaires were administered to all producers registered in British Columbia, Ontario, and Quebec in 2008. The response percentage was 33.2% (642 of 1,932). Nearly 70% of respondents rated the program as effective in producing safe chicken, and 49.1% rated the program requirements as easy to implement. Most respondents (92.9%) reported that they do not raise other poultry or keep birds as pets, and 79.8% reported that they clean and disinfect their barns between each flock cycle. Less than 50% of respondents reported that visitors wash their hands or change their clothes before entering barns, 38.4% reported that catching crews wear clean clothes and boots, and 35.8% reported that a crew other than from the hatchery places chicks. Respondents who rated the program requirements as effective or easy to implement were more likely to report the use of five of six highly recommended GPP. Only 21.1% of respondents indicated that Campylobacter can be transmitted from contaminated chicken meat to humans, and 26.6% believed that antimicrobial use in their industry is linked to antimicrobial resistance in humans. Continuing education of producers should focus on improving their awareness of these issues, while mandatory GPP should include those that are known to be effective in controlling Campylobacter and Salmonella in broiler chicken flocks.
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Joy, Jithin Ambarayil, Muhammad Sajjad, and Dong Won Jung. "Effect of Copper on Formability and Weldability of SUS 430J 1L Steel for Bead Forming of Drum." Key Engineering Materials 783 (October 2018): 159–64. http://dx.doi.org/10.4028/www.scientific.net/kem.783.159.

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Washing machine has greatly influenced people’s life styles by providing easy means of washing clothes and drying them out to a considerable extent. This not only saves time and amount of water used but also helps the user to wash and dry clothes with a lot of ease due to its fully automatic nature. But taking advantage of fully automated technology have some limitation, we have to take extra care during modeling of washing machine components and there selection of materials. In this paper we are studying about the change in formability and weldability properties of SUS 430J 1L steel sheet, by changing the amount of alloying elements for the manufacturing of washing machine drum beads. Two standard tests ASTM A751 - 14a and ASTM E8 / E8M – 16a is use for two types of samples, there comparison between mechanical and chemical properties and its effect on weldability and formability are discussed. From the results of the test, we find out that the formability is increased by increasing the amount of copper content in the steel sheet. Also by addition of more copper after some considerable amount negatively affect the weldability of the steel sheet.
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Boardman, John, and Robert Evans. "The measurement, estimation and monitoring of soil erosion by runoff at the field scale: Challenges and possibilities with particular reference to Britain." Progress in Physical Geography: Earth and Environment 44, no. 1 (July 16, 2019): 31–49. http://dx.doi.org/10.1177/0309133319861833.

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Soil erosion is widely acknowledged as a global problem but attempts to measure and estimate its significance are frustrated by our inability to develop reliable, cheap and easy methods of assessment. The limitations of qualitative methods such as GLASOD, errors and inaccuracies inherent in modelling based on small-scale plot experiments, and problems with 137Cs approaches, mean that alternative strategies are required. For runoff-related erosion on arable land we propose the use of a well-tried estimation technique: volumetric measurement of rills, gullies and fans. Amounts of wash and interrill erosion can also be estimated. This approach allows for the estimation of erosion rates at the field scale, rather than relying on extrapolations from plot-based data. Measurements are based on sampling the population of rills and gullies and can be adapted to the aims of the project for ‘broad-brush’ or detailed data. Monitoring of large areas to produce regional assessments of erosion risk is frequently required and volumetric estimates provide these data. Thus predictions of the extent, frequency and amounts of erosion can be made and the vulnerability of particular crops becomes clear.
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Stewart, Cristina, Kerstin Frie, Carmen Piernas, and Susan A. Jebb. "Development and Reliability of the Oxford Meat Frequency Questionnaire." Nutrients 13, no. 3 (March 12, 2021): 922. http://dx.doi.org/10.3390/nu13030922.

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Reliable and valid measurements of meat intake are needed to advance understanding of its health effects and to evaluate interventions to reduce meat consumption. Here, we describe the development and reliability of the Oxford Meat Frequency Questionnaire (MFQ). It asks individuals to report the number of servings of meat and seafood products they consumed in the last 24 h and is administered daily over 7 days. The MFQ combines food portion size data from the UK Food Standards Agency with estimates of meat content in composite dishes from the UK’s National Diet and Nutrition Survey. Adults who self-reported to eat meat (n = 129) completed a 3-week web-based test–retest reliability study assessing the MFQ twice, with a wash-out week in the middle. Two-way random intraclass correlation coefficients (ICC) revealed moderate to good agreement on all meat outcomes (total meat ICC = 0.716; minimum–maximum individual components = 0.531–0.680), except for fish and seafood (ICC = 0.257). Participants reported finding the questionnaire easy to use and, on average, completed it in less than 2 min. This new MFQ offers a quick, acceptable, and reliable method to measure changes in an individual’s meat intake in a UK population.
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emelda, emelda, Azizah Nada Septiawan, and Dinda Ayu Pratiwi. "FORMULASI DAN UJI SIFAT FISIK SEDIAAN GEL EKSTRAK ETANOLIK GANGGANG HIJAU (Ulva Lactuca LINN.)." Jurnal Insan Farmasi Indonesia 3, no. 2 (December 28, 2020): 271–80. http://dx.doi.org/10.36387/jifi.v3i2.645.

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One of the skin diseases that are often experienced by people is acne. The bacteria found in acne are P. acnes, S. aureus, and S. epidermidis. The preparation was chosen in this formulation because it is more practical and durable in storage. Gel preparations were chosen because it dry easily, can form a film layer that is easy to wash, and gives a cool feeling to the skin. Physical stability tests need to be carried out to ensure the quality, safety, and usefulness of the gel so that it meets the expected specifications and is stable in storage. This study aims to make a gel formulation and determine the physical properties of green algae Ethanolic extract gel. The optimum gel formula is determined based on variations in the concentration of Hydroxypropyl methylcellulose. The Gel that meets the criteria of homogeneity, consistency, pH , and dispersibility is determined as the optimum formula. The physical stability of the optimum formula was tested by organoleptic, homogeneity, pH test, spreadability test, and viscosity test. The gel that met the acceptance criteria was green algae ethanolic extract gel with a concentration of 3% HPMC so that it was determined as the optimum formula.
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Novrianti, Novrianti, Nani Apriyani, Achmad Imam Santoso, and Dhymas Sulistyono Putro. "Pembangunan Instalasi Cuci Tangan dalam Menurunkan Risiko Penyebaran Covid-19 di TPA Km. 14 Kota Palangka Raya." PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat 6, no. 1 (December 31, 2020): 15–18. http://dx.doi.org/10.33084/pengabdianmu.v6i1.1860.

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Scavengers have a risk of contracting Covid-19, which comes from the garbage when it is sorted. Based on this, it is necessary to make efforts to build a handwashing installation in the Km. 14 Landfill Palangka Raya City environment to prevent Covid-19, for the convenience of scavengers at work without the need for worries. The handwashing installation placement is based on the points that scavengers frequently pass so that it will be easier to access. This easy access will increase handwashing installations, which will potentially reduce the risk of Covid-19 spreads in the Km. 14 Landfill Palangka Raya City. The 250 L reservoir is placed on a wooden support. The placement is intended to facilitate the installation of water taps and when washing hands. The installation of water faucets is carried out in the 250 L reservoir provided by the 250 L reservoir producer. The sink is placed just below the water faucet outside, making it easier to wash hands and regulate water flow. The water flow from the collected hand washing will then flow gravitationally to the water filter that has been attached to the hose for further filtering so that when it flows into the country, the results of handwashing will not reduce the quality of the soil or groundwater.
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Alsulaili, Abdalrahman D., Mohamed F. Hamoda, Rawa Al-Jarallah, and Duaij Alrukaibi. "Treatment and potential reuse of greywater from schools: a pilot study." Water Science and Technology 75, no. 9 (February 20, 2017): 2119–29. http://dx.doi.org/10.2166/wst.2017.088.

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This study presented performance data on a low cost and easy maintenance pilot system for on-site treatment and reuse of water collected from wash sinks and fountains, as major sources of greywater (GW) at schools. Various treatment options were studied including screening, sand filtration, chlorination, and UV disinfection operated at different flow rates. Results showed that filtration operated at low rates is very effective in total suspended solids (TSS) removal, while UV proved to be more effective than chlorination for reduction of biochemical oxygen demand (BOD), chemical oxygen demand (COD), and total coliforms. Removal efficiencies up to 63%, 30% and 20% were obtained for TSS, COD and BOD, respectively and reductions of log TC (CFU/100 ml) from 6.5 to 2 were obtained at a filtration rate of 14 m3/d·m2. Treated effluent satisfied WHO standards for reclaimed water reuse in landscape irrigation and toilet flushing. The filtration-UV system is robust, showing the best and most reliable performance for low and high strength GW treatment even under a 10-fold increase in flow rate. A 5 m3/d pilot plant was developed for schools having 500 students and detailed cost-benefit analysis indicated a net saving value, a surplus of $1,600 per year, and pay back after 6 years and 11 months.
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Khari, Pooja, Sphruti Srivastava, and Shalini Mullick. "The Papanicolaou Society of Cytopathology Classification for Pulmonary Specimens : Application in Exfoliative Respiratory Cytology." Annals of Pathology and Laboratory Medicine 7, no. 9 (September 25, 2020): A434–438. http://dx.doi.org/10.21276/apalm.2863.

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Background: Cytologic examination of specimens obtained from the respiratory tract is the primary and frequently used diagnostic technique in patients with respiratory symptoms or in those presenting with a pulmonary abnormality. Lung carcinoma is the leading cause of mortality in India. Hence, early diagnosis and effective treatment are keys to prolong the survival of lung cancer patients. Methods: 100 consecutive samples were taken which included 66 samples of bronchial wash, 27 samples of brush and 7 samples of bronchoalveolar lavage. These were viewed independently by two pathologists to study the ease of applicability, reproducibility and role of Papanicolaou Society of Cytopathology classification for pulmonary specimens. Result: The classification and terminology scheme recommend a six-tiered system comprising of non-diagnostic, negative, atypical, neoplastic (benign and neoplasms of low malignant potential), suspicious and positive for malignancy. In our study 43% were non-diagnostic, 33% were negative, 10% were atypical, 6% were suspicious for malignancy and 8% were malignant. Conclusion: This classification system of respiratory cytology is easy to apply and interpret with minimal inter-observer variation. Standardized classification and terminology system provide a framework for consistent inter-intra departmental and inter institutional communication of diagnostic, prognostic and management information needed for consistent and optimal patient care.
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Coccè, Valentina, Anna Brini, Aldo Bruno Giannì, Valeria Sordi, Angiola Berenzi, Giulio Alessandri, Carlo Tremolada, Silvia Versari, Antonio Bosetto, and Augusto Pessina. "A Nonenzymatic and Automated Closed-Cycle Process for the Isolation of Mesenchymal Stromal Cells in Drug Delivery Applications." Stem Cells International 2018 (2018): 1–10. http://dx.doi.org/10.1155/2018/4098140.

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The adipose tissue is a good source of mesenchymal stromal cells that requires minimally invasive isolation procedures. To ensure reproducibility, efficacy, and safety for clinical uses, these procedures have to be in compliant with good manufacturing practices. Techniques for harvesting and processing human adipose tissue have rapidly evolved in the last years, and Lipogems® represents an innovative approach to obtain microfragmented adipose tissue in a short time, without expansion and/or enzymatic treatment. The aim of this study was to assess the presence of mesenchymal stromal cells in the drain bag of the device by using a prototype Lipogems processor to wash the lipoaspirate in standardized condition. We found that, besides oil and blood residues, the drain bag contained single isolated cells easy to expand and with the typical characteristics of mesenchymal stromal cells that can be loaded with paclitaxel to use for drug-delivery application. Our findings suggest the possibility to replace the drain bag with a “cell culture chamber” obtaining a new integrated device that, without enzymatic treatment, can isolate and expand mesenchymal stromal cells in one step with high good manufacturing practices compliance. This system could be used to obtain mesenchymal stromal cells for regenerative purposes and for drug delivery.
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Rantika, Nopi, Shilpa Fitria, Kharisma Putri N, Deska Syiami, and Dina Mulyani. "Health Education Program for Children in Godog Village-Garut As A Preventive Effort to the COVID-19 Virus." Indonesian Journal of Community Empowerment (IJCE) 1, no. 01 (August 31, 2020): 38–44. http://dx.doi.org/10.35899/ijce.v1i01.159.

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An epidemic of an acute respiratory disease called COVID-19 occurred at the end of 2019 in Wuhan (China) which spread throughout the world. COVID -19 can affect everyone from adults, the elderly, and children. So there needs to be education that is easy to carry out about Covid-19 and how to prevent it. This is the basis of the problem in encouraging community service activities so that they can be active in efforts to prevent COVID-19. The purpose of this educational activity is to prevent the spread of Covid-19 which is increasingly happening in Indonesia, so it is hoped that adults and children can take Covid-19 prevention. The method used during this activity is in the form of offline and online education to the surrounding community, where the material presented is in the form of proper handwashing and how to make hand sanitizers. As a result of the educational activities that have been carried out, there has been a significant change in the habits of the surrounding community where children can wash their hands properly and correctly, and for adults, especially local mothers can make hand sanitizers with the right natural ingredients to be used as emergency hand sanitizers. in daily activities.
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Spicuzza, Lucia, and Matteo Schisano. "High-flow nasal cannula oxygen therapy as an emerging option for respiratory failure: the present and the future." Therapeutic Advances in Chronic Disease 11 (January 2020): 204062232092010. http://dx.doi.org/10.1177/2040622320920106.

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Conventional oxygen therapy (COT) and noninvasive ventilation (NIV) have been considered for decades as frontline treatment for acute or chronic respiratory failure. However, COT can be insufficient in severe hypoxaemia whereas NIV, although highly effective, is poorly tolerated by patients and its use requires a specific expertise. High-flow nasal cannula (HFNC) is an emerging technique, designed to provide oxygen at high flows with an optimal degree of heat and humidification, which is well tolerated and easy to use in all clinical settings. Physiologically, HFNC reduces the anatomical dead space and improves carbon dioxide wash-out, reduces the work of breathing, and generates a positive end-expiratory pressure and a constant fraction of inspired oxygen. Clinically, HFNC effectively reduces dyspnoea and improves oxygenation in respiratory failure from a variety of aetiologies, thus avoiding escalation to more invasive supports. In recent years it has been adopted to treat de novo hypoxaemic respiratory failure, exacerbation of chronic obstructive pulmonary disease (COPD), postintubation hypoxaemia and used for palliative respiratory care. While the use of HFNC in acute respiratory failure is now routine as an alternative to COT and sometimes NIV, new potential applications in patients with chronic respiratory diseases (e.g. domiciliary treatment of patients with stable COPD), are currently under evaluation and will become a topic of great interest in the coming years.
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Muzyka, V. P., T. I. Stetsko, O. P. Panych, I. E. Atamanyuk, O. I. Chaykovska, O. Y. Kalinina, and H. P. Uhryn. "DISINFECTANTS FOR SANITARY TREATMENT OF THE SKIN OF THE ELDER OF LACTING COWS." Scientific and Technical Bulletin оf State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives аnd Institute of Animal Biology 22, no. 1 (March 29, 2021): 169–74. http://dx.doi.org/10.36359/scivp.2021-22-1.20.

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The article highlights the relevance of udder hygiene, sources of milk contamination with microflora, udder treatment requirements, sanitary condition control of the teats skin of cow udders, as well as a list of udder treatment products authorized in Ukraine. Proper preparation of udder teats for milking, namely: treatment with special detergents and disinfectants, compliance with the correct milking procedure significantly reduce the risk of cows with mastitis and increase milk yield. The content of microorganisms in milk shows the hygiene of milking cows, animal health, and the presence of pathogenic and technologically dangerous microorganisms. Bacterial contamination of milk is significantly increased due to udder diseases in cows. This increases the bacterial contamination of milk with pathogenic microbes, and the milk becomes unfit for consumption and processing (milk from cows with mastitis must be disposed of) milking procedure significantly reduce the risk of mastitis in cows and increase milk yield. It is necessary to prevent the penetration of microbes into the teat duct for prophylactic mastitis measures Special products should be used to treat the udder after milking. These products have certain requirements, the main of which are: the ability to quickly destroy bacteria; to keep the skin of the teats in good condition; to protect the teat between milking; easy to wash off before milking to eliminate the risk of getting into the milk.
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Utami, Nurul. "PENGARUH EDUKASI PERAWAT TERHADAP KETEPATAN KELUARGA PASIEN MELAKUKAN CUCI TANGAN DALAM PENCEGAHAN PENULARAN COVID-19 DI RSUD ARIFIN ACHMAD PROVINSI RIAU." Ensiklopedia of Journal 3, no. 4 (July 19, 2021): 70–74. http://dx.doi.org/10.33559/eoj.v3i4.807.

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Corona virus is a virus that attacks the respiratory system. The high incidence of Covid-19 is due to low public awareness of applying health protocols. So the need for prevention and control efforts by complying with health protocols to wear masks, keep your distance and wash your hands properly. Washing hands is an easy routine and is important in infection control, and is the best method to prevent the transmission of microorganisms. The purpose of this study was to determine the effect of nurse education on the accuracy of the patient's family through hand washing in preventing Covid-19 transmission at Arifin Achmad Hospital, Riau Province. This research uses quantitative research with a quasi experimental design, with a population of 20 people. Data collection tools used observation sheets and data analysis was carried out univariate and bivariate with the Wilcoxson test. The results of this study show that the average value before being given education about hand washing is low compared to the average value after being given education about hand washing. So that hand washing education is effective in increasing hand washing to prevent Covid-19 transmission with a p-value of 0.000. The results of this study are expected to be a source of information and knowledge for the nursing profession regarding the effect of providing nurse education on the accuracy of the patient's family in washing hands.
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Blanquer, Miguel, Valentín Cabañas-Perianes, Maria Juliana Majado, Victoria Sánchez Ibáñez, Pilar Menchón Sánchez, Jorge Monserrat, Alfonso Morales Lázaro, and Jose Maria Moraleda. "Evaluation of An Automatic Washing Method for DMSO Cryopreserved Peripheral Blood Hematopoietic Progenitors Using the Sepax S-100." Blood 114, no. 22 (November 20, 2009): 3219. http://dx.doi.org/10.1182/blood.v114.22.3219.3219.

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Abstract Abstract 3219 Poster Board III-156 Introduction Dimethylsulfoxide (DMSO) containing cryoprotective solutions are routinely used for the storage of hematopoietic progenitors (HP). At room temperature, DMSO is toxic for the cells and may produce severe adverse reactions during their infusion, especially in the pediatric patients. These problems can be avoided by washing the cells prior to the infusion. Our objective was to test if an automatic washing method (Sepax S-100, Biosafe) allowed us to preserve the CD34+ cell numbers with an adequate viability and engraftment potential. Material and Methods Forty five peripheral blood HP apheresis that have been cryopreserved using autologous plasma plus 9% DMSO were studied. After rapid thawing in a water bath at 37° C, an automatic wash with the Sepax S-100 (2 washes cycle) was performed. Nucleated cell levels determined by an hematology analyzer, flow cytometry CD34+ cell counts and Trypan Blue cell viability test were performed on aliquots collected prior to and after the washing technique. The paired Student's t-test and the Pearson's correlation coefficient were used for the statistical analysis. Results The mean total nucleated cell (TNC) and CD34+ cell recovery was 75,47% ± 3, and 94,66% ± 4,62 respectively. In spite of the TNC significant loss (p<0,001), there were no significant differences between the pre and post-washing CD34+ cell numbers (p=0,08) or viability (p=0,19). The TNC loss had no correlation with the pre or post-washing number of platelets (p=0,18 and p=0,16 respectively), neither with the loss of platelets during the procedure (p=0,31). In contrast with the 40% of untoward reactions recorded in our historical data of 226 DMSO containing cell infusions, we observed just one adverse effect (2,22%) with the washed cells. One patient reported a transient mild abdominal pain with nausea and one vomit. Median time to neutrophil engraftment (>500 cells /mL) and platelet engraftment (>50.000 cells/mL) were 11 ± 0,2 and 20 ± 1,4 days respectively. Conclusions The Sepax S-100 automatic wash protocol of DMSO containing peripheral blood progenitor cells determines a good CD34+ cell recovery and preserves their viability and engraftment potential. This method avoids the DMSO infusion related adverse events and it constitutes a closed and easy to do procedure. Disclosures No relevant conflicts of interest to declare.
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Supomo, Anita Apriliana, Titin Purnawati, and Ainur Risqi. "Formulation of Antiacne Cream Dosage Form Containing Mangosteen (Garcinia mangostana L.) Pericarp Ethanolic Extract." Indonesian Journal of Pharmaceutical and Clinical Research 1, no. 1 (April 16, 2018): 37–44. http://dx.doi.org/10.32734/idjpcr.v1i1.207.

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Abstract. The aim of this study was to formulate ethanolic extract of mangosteen (Garcinia mangostana L.) pericarp (EEMP) in the antiacne cream dosage form. The formulae were made with 3 varied concentrations of 0.5%, 1.5% and 4.5%. The tests carried out in this study were organoleptic cream preparations (odor, color, texture), homogeneity, pH, determination of the type of cream, spreadability, and the antibacterial activity against Staphylococcus epidermidis. The results of physical property evaluations showed that the EEMP indicated the pH value of 6, the spredability of the cream increased along with the increasing length of storage time. Duncan Test on the measurement of diameter dispersibility based on storage time showed that there were significant differences between the spreadability of the cream B and C. The cream produced was easy to wash indicating oil in water (o/w) type. The results of antibacterial activity test applying agar diffusion method using Mueller Hinton medium Agar (MHA) indicated that the inhibition zones of formulae II and III were 5.2 and 6 mm, respectively. Whereas, the formula I as well as the negative control showed no inhibition zones. It can be concluded that the EEMP can be formulated as an antiacne cream. The best cream formula was the formula II with the results of physical properties test were quite stable and able to produce inhibition zone against Staphylococcus epidermidis. Keywords: Garcinia mangostana, cream, antiacne, antibacterial, Staphylococcus epidermidis.
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Basseres, Anne, Bruno Verschuere, Gerard Holtzinger, Jean-Pierre Jacques, and Bernard Tramier. "A NEW CLEANING PRODUCT FOR OILED BIRDS AND AN INTEGRATED AUTOMATED PROCESS." International Oil Spill Conference Proceedings 1995, no. 1 (February 1, 1995): 171–75. http://dx.doi.org/10.7901/2169-3358-1995-1-171.

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ABSTRACT A new product for cleaning oiled birds was tested. A laboratory test was made in which several duck feathers, polluted with aged oil, were cleaned in 40° C water with the new product, then rinsed with cold water. Under these conditions, 90 percent of the oil was removed using the new product, versus 30 percent removal in control tests. Standard toxicity testing procedures were used to show the nontoxicity of the product. The new product was then tested using Sintef-Unimed techniques and metabolic activity tests on six mallard ducks. The cleaning process was easy and quick; birds recovered normal metabolic rate and thermal insulation one day after cleaning. Similar results were obtained on site in a rehabilitation center, where birds were also efficiently cleaned. The recovery rate for wild birds was about 60 percent after two weeks, with much higher hydrophobicity recovery rate when using the new product than when using the reference product. This new product for cleaning oiled birds appeared very efficient and nontoxic. A new technique to wash the birds also was tested on site. Accidently oiled wild birds were cleaned in an automated process, which included washing with the new product and efficient rinsing. The cleaning time was about 10 minutes for one bird. This technique was tested in one of the largest French rehabilitation centers. It appears very promising, considering the decrease in stress to the birds and the reduction in cleaning time.
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Sarkar, SK, and MK Uddin. "Multilayer cattle farming: a new dimension towards waste management and environmental sustainability in Bangladesh." Bangladesh Journal of Livestock Research 20, no. 1-2 (May 10, 2020): 101–11. http://dx.doi.org/10.3329/bjlr.v20i1-2.47024.

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An on-station study was conducted at demonstration farm of Rural Development Academy (RDA) Bogra, Bangladesh with objectives to determine the impact of multilayer (two storied) cattle farming on food security for achieving economical and environmental sustainability. A two storied shed for cattle rearing were built with 10:1 slope for easy drainage and disposal of urine, faeces, wash water, feed refusals to a biogas plant directly for anaerobic digestion. About 70-80% waste materials was automatically and the rest amount was transferred manually. Per annum total waste 787.67 tons was used and produced 28356 m3 biogas and 157.53 tons of organic manure. Gross return of Tk. 24,61,272 and gross profit of Tk. 1145672 was made on a capital investment for Tk. 13,15,600. The benefit cost ratio (BCR) found 1:1.87. Compared to conventional, multilayer farming significantly higher amount of fresh manure was collected. In conventional farming, on an average 39.4% of manure is lost due to rain and flood caused contamination of water and threat to public health. The analysis of per day income from a cow was significantly (p<0.01) higher in multilayer farming system compare to conventional system. The average income from manure was Tk. 24.14 and Tk. 4.62/cow, respectively. Thus, it may be concluded that multilayer cattle farming may be more profitable and effective for waste processing, reduction of pollution and sustainable development of environment. Bangladesh J. of Livestock Res. 20(1-2): 101-111, Jan-Dec 2013
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Piotrowicz, Zuzanna, Łukasz Tabisz, and Bogusława Łęska. "Material Balance for Stepwise Extraction of Freshwater Algae Biomass with Heavy Metal Sequestration." Ecological Chemistry and Engineering S 26, no. 4 (December 1, 2019): 675–85. http://dx.doi.org/10.1515/eces-2019-0003.

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Abstract During our studies concerning the isolation of sulfated polysaccharides (SPs) from freshwater algae species widespread in Poland, an improved overall extraction methodology has been developed. This new, multi-step procedure allows for obtainment of several fractions that could find use in cosmetic and food industries, with the final one containing the valuable SPs, important both from economic and scientific perspective. Moreover, the process allows for easy use of typical chelating agents to remove harmful heavy metal ions without important losses in natural compounds of interest. Furthermore, a material balance has been established for said process, allowing for its easier implementation on bigger scales, and highlighting the areas which could still be improved to positively affect the final time-cost ratio of the methodology. Combined with previously published information concerning the detailed composition of obtained fractions, we aim to provide a robust and informative outlook on the potential of native freshwater algae species as cheap, raw and easily purifiable resource, usable in a number of important industries. According to the mass balance, nearly 5 % of dry mass of Cladophora glomerata is extractable with ethanol and this fraction consists mostly of fatty acids, phenolics and pigments. Another 5 % of mass can be isolated as pure SPs from aqueous fraction. Additionally, calcium from natural incrustations on the surface of C. glomerata amounts to 17 % of dry material weight and can be reclaimed from acidic wash by simple precipitation; such calcium salts have garnered significant interest as nutritional supplements.
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45

Xiong, Li-Kun, and Cui-Yu Yin. "Preparation and characterization of cationic dyeable polycaprolactam modified with 5-sulfoisophthalic acid sodium and poly(ethylene glycol)." Textile Research Journal 87, no. 4 (July 21, 2016): 433–43. http://dx.doi.org/10.1177/0040517516631322.

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Unmodified nylon is dyeable to a single color only, and is almost exclusively dyed with acid dyes that are absorbed with amine groups of nylon molecules. Two types of polycaprolactam (PA6) copolyamide were successfully prepared with 5-sulfoisophthalic acid monosodium salt and poly(ethylene glycol) (PEG) units named cationic dyeable polyamide (CD-PA6) and easy cationic dyeable polyamide (ECD-PA6). The chemical and crystalline structures of CD-PA6 and ECD-PA6 were characterized by Fourier transform infrared spectroscopy and wide angle x-ray diffraction, and their thermal properties were tested by differential scanning calorimetry and thermogravimetric analysis, respectively. In addition, the rheological behavior and mechanical properties of copolyamide are presented in this paper. The influence of chemical modification in polyamide 6 fibers on the dyeing properties was investigated using cationic dye (methylene blue). The incorporation of PEG not only destroyed the regularity of the molecular chain arrangement and created more amorphous regions of ECD-PA6 samples, but also led to nylon 6 changing from the α-form to the γ-form. In addition, the crystallinities and degradation temperatures of samples which corresponded to different mass losses of CD-PA 6 and ECD-PA6 declined as the sulfonic group content increased, since large –SO3Na side groups in the copolymers prevented the chain molecules from tightly coagulating and obstructed the formation of larger crystals. Based on the analysis of the dyeing, a distinct improvement in tinctorial affinity and wash fastness for modified fibers compared with unmodified fibers was revealed.
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46

TODD, EWEN C. D., BARRY S. MICHAELS, JOHN HOLAH, DEBRA SMITH, JUDY D. GREIG, and CHARLES A. BARTLESON. "Outbreaks Where Food Workers Have Been Implicated in the Spread of Foodborne Disease. Part 10. Alcohol-Based Antiseptics for Hand Disinfection and a Comparison of Their Effectiveness with Soaps." Journal of Food Protection 73, no. 11 (November 1, 2010): 2128–40. http://dx.doi.org/10.4315/0362-028x-73.11.2128.

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Alcohol compounds are increasingly used as a substitute for hand washing in health care environments and some public places because these compounds are easy to use and do not require water or hand drying materials. However, the effectiveness of these compounds depends on how much soil (bioburden) is present on the hands. Workers in health care environments and other public places must wash their hands before using antiseptics and/or wearing gloves. However, alcohol-based antiseptics, also called rubs and sanitizers, can be very effective for rapidly destroying some pathogens by the action of the aqueous alcohol solution without the need for water or drying with towels. Alcohol-based compounds seem to be the most effective treatment against gram-negative bacteria on lightly soiled hands, but antimicrobial soaps are as good or better when hands are more heavily contaminated. Instant sanitizers have no residual effect, unlike some antimicrobial soaps that retain antimicrobial activity after the hygienic action has been completed, e.g., after hand washing. Many alcohol-based hand rubs have antimicrobial agents added to them, but each formulation must be evaluated against the target pathogens in the environment of concern before being considered for use. Wipes also are widely used for quick cleanups of hands, other body parts, and surfaces. These wipes often contain alcohol and/or antimicrobial compounds and are used for personal hygiene where water is limited. However, antiseptics and wipes are not panaceas for every situation and are less effective in the presence of more than a light soil load and against most enteric viruses.
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47

Dobs, Adrian S., Donald R. Hoover, Min-Chi Chen, and Richard Allen. "Pharmacokinetic Characteristics, Efficacy, and Safety of Buccal Testosterone in Hypogonadal Males: A Pilot Study1." Journal of Clinical Endocrinology & Metabolism 83, no. 1 (January 1, 1998): 33–39. http://dx.doi.org/10.1210/jcem.83.1.4505.

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Transbuccal administration of drugs provides an easy route of administration. To test the safety and efficacy of a novel testosterone (T) product, we performed a randomized, double blind, placebo-controlled study in a parallel design. Men with serum T levels below 250 ng/dL were administered either an active buccal tablet containing 10 mg T (n = 7) or a buccal placebo tablet (n = 6) containing 3 mg pseudoephedrine HCl for taste matching. Men were studied while taking a standard T enanthate dose, after 6 weeks of a wash-out period, and after 8 weeks of therapy. The men were matched for age (mean ± sd, 41 ± 16 vs. 47 ± 16) and type of hypogonadism (three primary testicular failures in each group, with the remainder having a central etiology). Acute pharmacokinetic testing showed peak serum hormone levels at 30 min, with a mean serum T concentration of 2688 ± 147 ng/dL (range, 1820–3770 ng/dL). Levels returned to baseline in 4–6 h, resulting in a total T area under the curve level of 3865 ng/hn·dL, less than that using other available forms of T administration. Similar pharmacokinetics were observed for the hormone’s metabolites, bioavailable T, free T, and estradiol. After 8 weeks of treatment, the results of nocturnal penile tumescence studies evaluating rigidity and circumference were significantly different from those in the placebo group (P &lt; 0.05) and comparable to those during T enanthate therapy. In conclusion, transbuccal T therapy was sufficient to maintain normal sexual function while minimizing the total time of exposure to elevated circulating serum T levels.
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48

Divyalakshmi, D., N. Kumaravelu, Thanga Thamil Vanan, and P. Tensingh Gnanaraj. "Comparison of floor bacterial load in different types of rabbit cages." Journal of Applied and Natural Science 10, no. 4 (December 1, 2018): 1226–29. http://dx.doi.org/10.31018/jans.v10i4.1916.

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Rabbits are housed on various types of cages including single tier, multi- tier cages etc. The effect of housing system on the floor bacterial load is variable, and its effect on animal production is limited. An attempt has been made to evaluate and compare the floor bacterial load between single-tier and multi-tier caging system in rabbitary. The study was carried out in rabbitary which housed broiler rabbits. The samples were taken in sterile cotton tipped swabs. The floor bacterial load was determined by dilution method where serial dilutions were done with sterile normal saline. An aliquot of 1 ml was taken from dilution and poured in sterile petri plates in triplicate and mixed with liquefied sterilized plate count agar. Following incubation, the bacterial colonies were counted. The bacterial load in the single-tier was comparatively lower than the multi-tier cage even before washing of cages. There was significant difference (P<0.01) in the floor bacterial load before (1.73x1012±0.30) and after water wash (1.35x1011±0.29) and between single (1.50x109±0.30) and multi-tier cages (1.35x1011±0.29). The bacteria up to genes level found using Hi-Media bacterial kit were Staphylococcus aureus, Staphylococcus epidermidis, Flavobacterium sp, Micrococcus sp, Proteus sp, Mannheimia sp, Klebsiella sp, Bordetella sp, Bacillus sp, Actinomyces sp, Cornybacterium sp and Pseudomonas sp. This study concluded that reduced bacterial load found in single tier may be due to good aeration and better handling facilities. In single tier cage the dung and urine gets settled at the floor which facilities easy washing which slows down the bacterial growth.
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Feng, Ding, Xiao Fei Chang, Xian Yong Zhang, Shou Yong Li, Chao Ruan, and Yu Xie. "The Research to Hydrocyclone Desander of Sand Removing Based on Crude Oil." Applied Mechanics and Materials 152-154 (January 2012): 1336–41. http://dx.doi.org/10.4028/www.scientific.net/amm.152-154.1336.

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The fractional volume of the reservoir sand shall be taken to the ground when the crude oil is brought up from the ground. With the deepening of the oil extraction the sand content of oil recovery is increasing. Although many measures have been used such as prevent sand, block sand and so on, the viscosity of heavy oil is big and the fractional of sand is carried into the crude oil gathering system inevitably, causing a series of problems at ground equipment, having a serious impact on the normal production and gathering of crude oil. The larger size of sand can be removed by the sedimentation and the smaller sand can be removed by hydrocyclone devices. Hydrocyclone is a separation plant used to separate non-uniform phase mixtures. It can be used to complete the liquid clarification and to wash particles, liquid degassing and grit removal, grading and classification of solid particles and the separation of two non-miscible liquids and others. Hydrocyclone separation technique is simple and convenient operation, high separation efficiency, no rotating units, small size and easy to realize automatic control. On the offshore platform,if the sand mixed with oil is discharged into sea unsatisfactory the standard, it will pollute the sea. It will cause a serious of marine pollution; this is a problem that should be solved quickly. This paper discusses the requirements for the design of the hydrocyclone desander equipment with compact structure, applicable to offshore drilling platforms,and carrying out CFD simulation, the results showing that the particle size of 75 of sand, grit removal efficiency above 90%.
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50

Junkes-Cunha, Maíra, Anamaria Fleig Mayer, Cardine Reis, Abebaw M. Yohannes, and Rosemeri Maurici. "The Manchester Respiratory Activities of Daily Living questionnaire for use in COPD patients: translation into Portuguese and cross-cultural adaptation for use in Brazil." Jornal Brasileiro de Pneumologia 42, no. 1 (February 2016): 15–21. http://dx.doi.org/10.1590/s1806-37562016000000029.

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Objective : To translate The Manchester Respiratory Activities of Daily Living (MRADL) questionnaire into Portuguese and to create a version of the MRADL that is cross-culturally adapted for use in Brazil. Methods : The English-language version of the MRADL was translated into Portuguese by two health care researchers who were fluent in English. A consensus version was obtained by other two researchers and a pulmonologist. That version was back-translated into English by another translator who was a native speaker of English and fluent in Portuguese. The cognitive debriefing process consisted in having 10 COPD patients complete the translated questionnaire in order to test its understandability, clarity, and acceptability in the target population. On the basis of the results, the final Portuguese-language version of the MRADL was produced and approved by the committee and one of the authors of the original questionnaire. Results : The author of the MRADL questioned only a few items in the translated version, and some changes were made to the mobility and personal hygiene domains. Cultural differences regarding the domestic activities domain were found, in particular regarding the item "Do you have the ability to do a full clothes wash and hang them out to dry?", due to socioeconomic and climatic issues. The item "Do you take care of your garden?" was questioned by the participants who lived in apartments, being modified to "Do you take care of your garden or plants in your apartment?" Conclusions : The final Portuguese-language version of the MRADL adapted for use in Brazil was found to be easy to understand and easily applied.
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