Dissertations / Theses on the topic 'Echites'
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Duerr, Heike Edith. "Molecular characterisation of neuropeptides in echinoderms." Thesis, Royal Holloway, University of London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314294.
Full textBosdet, Ian Edward. "Identification of echinus and characterization of its role in Drosophila eye development." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/1418.
Full textBishop, G. M. "Aspects of the reproductive ecology of the sea urchin Echinus esculentus L." Thesis, University of Exeter, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.353043.
Full textPaine, Mark John Ingraham. "Molecular cloning of Echis carinatus venom genes." Thesis, University of Liverpool, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.291952.
Full textVost, L. M. "The influence of grazing by the sea urchin Echinus esculentus L. on subtidal algal communities." Thesis, University of Liverpool, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372713.
Full textLaing, Gavin. "The use of liposomes for immunisation against Echis carinatus venom." Thesis, University of Liverpool, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317199.
Full textHowes, Joanna-Marie. "The purification, characterization and inhibition of three metalloproteinases from Echis ocellatus venom." Thesis, University of Liverpool, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.396763.
Full textMorales, Juan F. [Verfasser], and Sigrid [Akademischer Betreuer] Liede-Schumann. "Systematics of the tribe Echiteae and the genus Prestonia (Apocynaceae, Apocynoideae) / Juan F. Morales ; Betreuer: Sigrid Liede-Schumann." Bayreuth : Universität Bayreuth, 2017. http://d-nb.info/1132200970/34.
Full textCasewell, Nicholas Robert. "The genetic basis of venom variation in the genus echis : Causes, correlates and consequences." Thesis, Bangor University, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.528332.
Full textIddon, Dale. "Application of hybridoma technology to the study of West African Echis carinatus (Carpet viper) venom poisoning." Thesis, University of Liverpool, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303688.
Full textHasson, Sigdi S. Anwer Abdo. "DNA immunisation to generate snake antivenom : cloning and characterisation of novel cDNAs encoding haemostasis-disruptive venom toxins of saw scaled viper, Echis ocellatus." Thesis, University of Liverpool, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.403219.
Full textRevault, Pascal. "Serpents, savoirs et santé chez les Mossi : prise en charge des envenimations par Echis ocellatus en Afrique soudano-sahélienne à travers l'exemple du plateau ouagalais." Paris 13, 1994. http://www.theses.fr/1994PA130034.
Full textDENG, DA-QI, and 鄧達祺. "The breeding in early larvae of the sea cucumber Actionpyga echinites." Thesis, 1993. http://ndltd.ncl.edu.tw/handle/69887478285604405644.
Full textLiu, Cheng-Hong, and 劉正弘. "Multiple Tips Echinus-like Gold Nanoparticles: Fabrication Route and their Growth Mechanistic Investigations." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/48517813144538958285.
Full text國立清華大學
化學系
98
Abstrat In this study, we use the material containing bis(quaternary ammonium bromide) surfactant DC14TAB to promote formation of multiple tips echinus-like gold nanostructure. For the growth mechanism, we find that the lipid-like property of this surfactant promotes the formation of echinus-like structure. From the experiment result, this structure may be formed first by aggregation of small nanoparticles, followed by growth into multiple tips echinus structure in the presence of dimeric surfactant DC14TAB as the structure- directing agent. According to the lightning rod effect, multiple long tips structure on the surface of this particle will produce strong local electric field, this property can be used to enhance the Raman signal of surface absorbed organic molecule on the Au nanoechinus . We compare the intensity of Raman signal generated from Au nanoechinus with that from spherical Au NPs. The good signal enhancement of this particle show that it would be the potential candidate for the Raman signal enhancement substrate.
Chen, Yuh-Ling, and 陳玉玲. "Heterodimeric anticoagulant proteins from Echis carinatus ter venom: amino acid sequence and functionaln." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/80755655977767044486.
Full textChen, Yu-Ling, and 陳玉玲. "Heterodimeric anticoagulant proteins from Echis carinatus ter venom: amino acid sequence and functionaln." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/07084497342501643236.
Full textKemparaju, K. "Characterisation of immunologically cross-reacting phospholipases A2 toxins from Indian Saw-scaled viper (ECHIS CARINATUS) venom." Thesis, 1995. http://hdl.handle.net/2009/1873.
Full textKemparaju, K. "Characterization of immunologically cross-reacting Phospolipase A2 Toxins from Indian Saw Scaled Viper (Echis Carinatus) Venom." Thesis, 1995. http://hdl.handle.net/2009/1333.
Full textKuo, Heng-Lan, and 郭姮蘭. "Action Mechanism of Echisaggretin, a Platelet Aggregation Inducer Purified from Snake Venom of Echis carinatus sochureki." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/27504602294713842727.
Full text國立臺灣大學
藥理學研究所
89
English Abstract By using column chromatography of FPLC Mono S, the active platelet aggregation inducer, echisaggretin, was purified from Echis carinatus sochureki snake venom.Under the non-reducing condtion of 15 % SDS-PAGE, the molecular weight of echisaggretin was estimated to be about 28 kDa. Under the reducing condition with 2% β-mercaptoethanol, echisaggretin was shown to contain two subunits with molecular weight of about 15 kDa and 13 kDa, respectively. The platelet aggregation and ATP release reaction in human platelet elicited by echisaggretin follows the all-or-none manner. When the concentration used was greater than the concentration (1μg/ml), it caused the maximal aggregation and ATP release reaction. The monoclonal antibody 6F1 raised against α2 integrin or AP1, 6D1 or agkistin which are GPIb antagonists partially inhibit echisaggretin-induced platelet aggregation, but 6F1 in comination with AP1, 6P1 or agkistin completely abolished echisaggretin-induced platelet aggregation .TXA2 formation plays a important role in collagen and echisaggretin-induced platelet aggregation because platelet aggregation induced by them were significantly attenuated by indomethacin. ADP release reaction has minor role in echisaggretin-induced platelet aggregation, because CP/CPK and apyrase have no effect on it, but it is very important for collagen-induced platelet aggregation. Neomycin, staurosporine, Ro 31-8220, calphostin C, tyrphostin A47, BAPTA/AM, EGTA, EDTA, LY294002 and cytochalasin D caused obvious inhibition on platelet aggregation triggered by collagen and echisaggretin, indicating that the activation of PLC, PKC, tyrosine kinases, cytoskeleton, exogenous calcium ion and mobilization of intracellular calcium play important roles on their functions. In conclusion, the binding site of echisaggretin on platelet membrane might be the GPIa/IIa(α2β1 integrin) and GPIb. Their actual binding sites remain to be investigated. The activation of PLCγ2, PKC, PI3-kinase, cytoskeleton and tyrosine kinases as well as calcium mobilization were all important for echisaggretin-induced platelet aggregation, but their interactions still need further research.
Lo, Pei-Ching, and 羅珮菁. "Effects of Echiquatin, a Glycoprotein Ib Binding Protein from the Venom of Echis multisquamatus, on Platelet Function." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/40565935143843005655.
Full text國立臺灣大學
藥理學研究所
91
By using column chromatography of CM-sephadex C-50 and Mono-S ionic exchanger in sequence, a novel snake venom protein, echiquatin, was purified from Echis multisquamatus snake venom. Echiquatin inhibited ristocetin-induced platelet agglutination mediated by von Willebrand factor (vWF)-GPIb interaction. Echiquatin was homogenous as judged by SDS-PAGE. Its apparent molecular weight was estimated to be 30,800 daltons as measured by SDS-PAGE. Upon reduction by 2% beta-mercaptoethanol, echiquatin was shown to contain two subunits with molecular weight of 14,800 (alpha-chain) and 13,100 (beta-chain) daltons, respectively. It is devoid of enzymatic activity as assayed by fibrinogenolysis and azocaseinolysis. Echiquatin concentrationdependently inhibited ristocetin (1 mg/ml)induced platelet agglutination in the presence of plasma (20ul) as the source of vWF in platelet suspension. Echiquatin (1.5ug/ml, 49 nM) completely inhibited ristocetin-induced platelet agglutination. The antiplatelet activity of echiquatin was not inhibited by preincubating it with 10 mM EDTA, suggesting that echiquatin is not a venom metalloproteinase and its inhibitory effect is not bivalentcation dependent. Echiquatin only slightly inhibited collagen (10ug/ml), and TRAP (20uM) induced platelet aggregation, but it obviously prolonged the latent period in triggering aggregation. Echiquatin did not inhibit thrombin (0.1, 0.03 U/ml)induced platelet aggregation, but it also prolonged the latent period in triggering aggregation. Even at a high concentration of 20ug/ml, echiquatin had no significant effect on platelet aggregation caused by U46619 (1uM), and ADP (20uM). Using flow cytometry, echiquatin blocked the binding of AP1 and 6D1, mAbs against platelet GPIb, to platelet. However, it showed no significant effects on the binding of mAb, raised against platelet GPIb (CLB-MB45), GPIX, GPIIb or GPIa/IIa (6F1). Echiquatin dosedependently prolonged the bleeding time of ICR mice after intravenous injection. When a dose of echiquatin (0.35ug/mouse) given, a decrease of platelet count (39.98%) was observed at the first 5 min, however the platelet count gradually returned to control level within 6 hr. Platelet agglutination was observed with blood smears obtained from the coincubation of the whole blood with echiquatin. Echiquatin concentrationdependently induced platelet agglutination in plateletrich plasma. Echiquatin (1.25ug/ml) induced maximum platelet agglutination, and its activity was inhibited by AP1 and 6D1, mAbs against platelet GPIb, but not by mAbs of SZ2, 6F1 and 7E3, raised against GPIb, GPIa/IIa or GPIIb-IIIa, respectively. In the presence of plasma, echiquatin induced platelet agglutination of platelet suspension, suggesting that some plasma factor(s) are involved in triggering platelet agglutination in cooperation with echiquatin. Using ELISA technique, we demonstrated that echiquatin specifically binds to IgMkappa, but not IgMlambda. In conclusion, echiquatin inhibits ristocetin-induced agglutination in human platelet suspension, but induces agglutination in plateletrich plasma. Binding study with flow cytometry has demonstrated that echiquatin selectively inhibits AP1 and 6D1 binding to platelets, suggesting that echiquatin selectively binds to platelet surface receptorGPIb, thereby blocking the interaction between GPIb and vWF. According to ELISA experiment, echiquatin binds to plasma IgMkappa, a pentamer molecule, thus leading to a complex formation among platelet GPIb, echiquatin and IgMkappa and subsequently platelet agglutination. The phenomena may explain the in vivo thrombocytopenia effect of a GPIb binding protein, echiquatin. The elucidation of the structureactivity relationship of echiquatin and other GPIb binding proteins at a molecular level would be helpful for developing a new group of antithrombotic drugs.
Hung, Yu Chun, and 洪幼軍. "Purification and characterization of the platelet glycoprotein Ib antagonist, Echiquatin, a snake venom protein from Echis multisquamatus." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/39418031847381266348.
Full textHsieh, ChiWen, and 謝綺文. "Purification of Echisotin, a Snake Venom Peptide from Echis carinatus sochureki, and Its Effects on Pletelet and Chondrocyte." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/19762652043143424312.
Full text國立臺灣大學
藥理學研究所
87
英文摘要 1. By means of column chromatography of CM-Sephadex C-50, gel filtration of Sephadex G-100 and Sephadex HR 10/30, and finally HPLC of a C18 reverse phase column, a new snake venom protein, echisotin, was purified from the venom of Echis carinatas sochureki. 2. Echisotin was homogenous as judged by SDS-PAGE and N-terminal amino acid analysis. The purified protein showed a single-band with an apparent molecular weight of 5,600 daltons under both the reduced and non-reduced conditions. Following S-alkylation and reduction, the N-terminal amino acid sequence of echisotin was identified. Echisotin contained an Arg-Gly-Asp (RGD) sequence at position 24~26 and was highly homologous with those of the recently reported members of disintegrin family. 3. In human platelet-rich plasma, echisotin dose-dependently inhibited the platelet aggregation induced by collagen (10 mg/ml) and ADP (20 mM). The IC50 values were estimated to be 3.44 mg/ml (0.61 mM) and 1.12 mg/ml (0.20 mM), respectively. In human platelet suspension, echisotin also exhibited an inhibitory effect on the aggregation of platelets stimulated by collagen (10 mg/ml), thrombin (0.1 U/ml), U46619 (1 mM) and ADP (20 mM) in the presence of fibrinogen (20 mg/ml) in a concentration-dependent manner, with IC50 values of 0.92 mg/ml (0.16 mM), 0.77 mg/ml (0.14 mM), 0.53 mg/ml (0.09 mM) and 0.35 mg/ml (0.06 mM), respectively. However, echisotin apparently did not affect the shape change caused by these agonists. 4. In the studies of cell adhesion to ECMs, echisotin inhibited on HUVEC adhesion to immobilized fibrinogen (40 mg/ml) and fibronectin (40 mg/ml) in a dose- and RGD-dependent manner. The IC50 values were estimated to be 12.92 mg/ml (2.31 mM) and 11.48 mg/ml (2.05 mM), respectively. In addition, echisotin also concentration-dependently inhibited IRC adhesion to immobilized fibrinogen and fibronectin with IC50 values of 10.23 mg/ml (1.83 mM) and 10.02 mg/ml (1.79 mM), respectively. However, Integrelin (a specific integrin aIIbb3 antagonist) showed an inhibitory effect on HUVEC adhesion to these ECMs at 120.2 mM, but it showed little effect on IRC adhesion. 5. The effects of echisotin on the binding or many anti-integrin monoclonal antibodies (mAbs) were examined by flow cytometry. Echisotin specifically inhibited the binding of anti-b3 integrin mab to IRC in a dose-dependent manner, but not those of other anti-integrin mAbs such as b1 and a5 . However, in human platelet and HUVEC preparations, echisotin concentration-dependently blocked the 7E3 interaction with these cells. 6. The effects of echisotin on growth factors stimulated rat chondrocyte proliferation were evaluated by using MTT assays. Pretreatment of echisotin dose-dependently inhibited the proliferation of IRC by exposure to either PDGF or TGF-b1 (both in 20 ng/ml) for 24-h. The IC50 values were estimated to be 3.08 mg/ml (0.55 mM) and 2.61 mg/ml (0.41 mM), respectively. 7. In conclusion, echisotin, belonging to the membrane of disintegrin family, modulates the functional behaviors of chondrocyte, endothelial cells and platelets by interacting with b3 integrin expressed on these cells. The working mechanisms of the anti-platelet aggregation, anti-adhesive and anti-proliferative activities of echisotin may be closely related to integrin b3 receptor blockade. However, the interactions of signal transduction induced by growth factor and integrin require further investigation. In addition, it remains to be elucidated regarding the regulation of cellular signaling pathway by echisotin in modulating cell functional behaviors.
Chai, Chia-Hsiang, and 柴佳翔. "Effects of Echispydin, a New Disintegrin Purified from Snake Venom of Echis pyramidium, on Platelet and Vascular Cells." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/41729511220176593637.
Full text國立臺灣大學
藥理學研究所
87
Echispydin, a 48 amino-acid protein, which inhibits platelet aggregation, is a new member of disintegrin family derived from the venom of the saw-scaled viper Echis pyramidium. The purification procedure consisted of ion-exchanger chromatography, gel filtration on Sephadex G-100, and C18 reverse-phase high pressure liquid chromatography. The protein was homogenous as judged by SDS-PAGE and NH2-terminal sequence analysis. Echispydin was shown as a short-chain polypeptide with a molecular weight of 5,081 daltons. The sequence of echispydin shows 85﹪identity to the echistatin, a known short-chain disintegrin from the Echis carinatus. It also contains the sequence Arg-Gly-Asp (RGD) which is responsible for its binding to the glycoprotein IIb/IIIa of platelets. Echispydin inhibited thrombin, collagen, and U 46619-induced human platelet aggregation in a dose dependent manner. The IC50 was estimated to be 1.15 micro-g/ml (0.23 micro-M), 1.22 micro-g/ml (0.24 micro-M), and 0.94 micro-g/ml (0.19 micro-M), respectively. Using flow cytometry, the binding of FITC-conjugated echispydin to platelets and vascular smooth muscle cells were measured. In this report we found that echispydin, specifically inhibited the binding of monoclonal antibody 7E3, which recognizes integrin alphaIIb beta3, to human platelets in a dose-dependent manner. Moreover, the binding of FITC-echispydin to rat vascular smooth muscle cells was abolished by F11, a monoclonal antibody raised against 3 integrin. Regarding the functional studies, echispydin inhibited the adhesion of human umbilical vein endothelial cells (HUVECs) and rat aortic smooth muscle cells (RASMCs) to immobilized extracellular matrix (ECM, i.e. fibrinogen, fibronectin, and vitronectin) in a RGD dependent manner. The migration of vascular smooth muscle cells is thought to play a key role in the pathogenesis of many vascular diseases (e.g. atherosclerosis) and is modulated by soluble growth factors as well as interactions between integrins and ECMs. We also studied the effect of echispydin on the PDGF-directed migration of RASMC. Echispydin can binds to integrin v3 and significantly inhibited cell migration. These observations suggest that echispydin may be an useful research tool and helpful for developing new drugs in thrombogenic diseases.
Köck, Stefan [Verfasser]. "Das Juhō-yōjin-shū [Sammlung von der Wachsamkeit beim Empfangen buddhistischer Lehren] des Mönches Shinjō als Apologie der Shingon-Schule gegenüber konkurrierenden Lehren in der Provinz Echizen um 1270 / vorgelegt von Stefan Köck." 2007. http://d-nb.info/999495364/34.
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