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Journal articles on the topic 'EcoR1'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Kodogo, Vitaris, Danai Tavonga Zhou, Olav Oektedalen, Kerina Duri, Babill Stray-Pedersen, and Exnevia Gomo. "Apolipoprotein B Gene Polymorphisms and Dyslipidemia in HIV Infected Adult Zimbabweans." Open AIDS Journal 10, no. 1 (2016): 190–98. http://dx.doi.org/10.2174/1874613601610010190.

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Background:Dyslipidemia does not occur in all HIV-infected or antiretroviral therapy-experienced patients suggesting role of host genetic factors but there is paucity of data on association between dyslipidemia and gene polymorphisms in Zimbabwe.Objective:To determine association of lipoprotein levels andapolipoprotein Bpolymorphisms in HIV-infected adults.Method:Demographic data were collected from 103 consenting patients; lipoprotein levels were determined and blood samples were successfully genotyped for bothapolipoprotein B2488C>T Xba1 andapolipoprotein B4154G>A p.Gln4154Lys EcoR1 po

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Mohammed, Abdulsalam Najm, Tasnim Tariq Mohammed, Harith K. Buniya, and Dhafer F. Al –Rawi. "The Identification and Characterization of the Resistance Genes of Klebsiella pneumoniae Plasmids Transformed in E. coli BL21(DE3)." Research Journal of Biotechnology 19, no. 11 (2024): 215–21. http://dx.doi.org/10.25303/1911rjbt2150221.

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The emergence of multidrug-resistant Klebsiella pneumoniae constitutes a serious threat to global public health due to the limited treatment options available. This study aimed to identify some characteristics carried on the plasmids of K. pneumoniae isolated from pathological cases. The plasmid was extracted from K. pneumoniae and transformed into E. coli BL21(DE3) by the heat shock method using 0.1 M CaCl2. The results showed that transformed E. coli BL21(DE3) became resistant to four types of antibiotics which included cefepime (FEP) 10 μg, ceftazidime (CAZ) 30 μg, tetracycline (TE) 10 μg a

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3

Puspaningsih, Ni Nyoman Tri, Akhmaloka Akhmaloka, Sofyan Hadi, Y. Sri Wulan Manuhara, and Bambang Irawan. "ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI ESCHERICHIA COLI DH5A." Berkala Penelitian Hayati 4, no. 2 (1999): 61–67. http://dx.doi.org/10.23869/bphjbr.4.2.19992.

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Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert. Characterization of the recombinant DNA by Stu1 and EcoR1 restriction enzymes indicated nucleotide sequence of insert gene. Digestion by BamHI, Cla1, and EcoRV restriction enzymes showed only one band that assumed size of insert gene is about 1500 bp. Gene expression showed that amylase enzyme activity by using Somogyi-Nelson method was 88.0265 Unit. This activity was 10 percent higher than transformant cont

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4

Bailey, Karen M., and D. M. West. "Restriction endonuclease (EcoR1) analysis ofBrucella ovisDNA." New Zealand Veterinary Journal 35, no. 10 (1987): 161–62. http://dx.doi.org/10.1080/00480169.1987.35428.

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5

Zhu, Yi, Hu Zeng, Xiang Gao, and Zuhong Lu. "Quantitative analysis of EcoR1 methylase-DNA complex by atomic force microscopy." Scanning 28, no. 1 (2006): 15–19. http://dx.doi.org/10.1002/sca.4950280103.

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6

Johnston, S. D., C. López-Fernández, F. Arroyo, R. Roy, W. V. Holt, and J. Gosálvez. "Protamine composition of koala and wombat spermatozoa provides new insights into DNA stability following cryopreservation." Reproduction, Fertility and Development 31, no. 10 (2019): 1558. http://dx.doi.org/10.1071/rd18512.

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To investigate differences in the post-thaw DNA stability of koala and wombat spermatozoa, protamine amino acid sequences were compared and it was found that there were three more arginine residues for the wombat. Koala and wombat spermatozoa, cryopreserved using identical protocols, were examined for changes in sperm DNA fragmentation (SDF) dynamics over 24h of post-thaw incubation. Following validation of a wombat sperm chromatin dispersion test, wombat DNA showed a rate of SDF that was 6-fold higher than for koala spermatozoa (P=0.038). Finally, we examined whether expected differences in c

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7

Hill, A. W., and J. A. Leigh. "DNA fingerprinting ofStreptococcus uberis: a useful tool for epidemiology of bovine mastitis." Epidemiology and Infection 103, no. 1 (1989): 165–71. http://dx.doi.org/10.1017/s0950268800030466.

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SUMMARYA simple and reproducible typing system based on restriction fragment size of chromosomal DNA was developed to compare isolates ofStreptococcus uberisobtained from the bovine mammary gland. The endonuclease giving the most useful restriction patterns wasHindIII, although seven other endonucleases (Bgl1,EcoR1,Not1,Pst1,Sfi1,Sma1,Xba1) were also tested in the system. An image analyser was used to obtain a densitometric scan and a graphic display of the restriction patterns. Such a system will allow large scale data storage for future computer-aided comparison.

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Nagesha, N., J. Natesh, N. H. Meenakshi, Anitha Peter, and V. Venkataravanappa. "Diversity analysis of BmNPV Isolates Infecting Mulberry Silkworm (Bombyx mori) using Restriction Endonuclease Digestion Profiling." International Journal of Bio-resource and Stress Management 13, no. 1 (2022): 1–8. http://dx.doi.org/10.23910/1.2022.2352.

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Understanding the genetic diversity of BmNPV isolates causing grasserie viral disease of mulberry silkworm Bombyx mori L. is essential for adoption of management strategies including biotechnological tools. The present study was aimed at the use of restriction fragment length polymorphism (RFLP) profiling for studying the molecular diversity analysis of six BmNPV isolates collected from Devanahalli, Kolar, Shidlaghatta, Hosakote, Tumakuru, and Ramanagara areas in Karnataka, India (BmNPV-Ko, BmNPV-Ho, BmNPV-SG, BmNPV-DH, BmNPV-TM, BmNPV-Ram respectively). DNA was extracted from each of these is

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9

LUKE, PETER A., and STEPHEN E. HALFORD. "Fluorescent labelling of EcoRI and EcoRV." Biochemical Society Transactions 14, no. 2 (1986): 259–60. http://dx.doi.org/10.1042/bst0140259a.

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10

Plante, Yves, Peter T. Boag, and Bradley N. White. "Nondestructive sampling of mitochondrial DNA from voles (Microtus)." Canadian Journal of Zoology 65, no. 1 (1987): 175–80. http://dx.doi.org/10.1139/z87-025.

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We present two techniques for sampling mitochondrial DNA (mtDNA) without killing individual voles. Total cellular DNA was extracted from small blood samples (100–250 μL) and tail segments (2 cm long) collected from meadow voles (Microtus pennsylvanicus). Restriction fragment patterns produced by the restriction endonucleases HindIII, BamH1, and EcoR1 after hybridization with a probe of nick-translated mtDNA compared well with standard mtDNA assays. Both techniques can be used in the field, and should prove useful in biochemical taxonomy as well as in investigations of population structure, dis

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11

Doughty, B., S. W. Kazer, and K. B. Eisenthal. "Binding and cleavage of DNA with the restriction enzyme EcoR1 using time-resolved second harmonic generation." Proceedings of the National Academy of Sciences 108, no. 50 (2011): 19979–84. http://dx.doi.org/10.1073/pnas.1115498108.

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12

Nascimento, Elmiro R., Al J. DaMassa, Richard Yamamoto, and M. Graça F. Nascimento. "Plasmids in Mycoplasma species isolated from goats and sheep and their preliminary typing." Revista de Microbiologia 30, no. 1 (1999): 32–36. http://dx.doi.org/10.1590/s0001-37141999000100006.

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One-hundred-five (105) clinical isolates of mycoplasma from caprine origin and one isolate from ovine were surveyed for plasmids, which were present in thirty-three (31%) of them. These mycoplasmas originated from 13 herds. Ten of them were symptomatic for mycoplasmal disease (mastitis, polyarthritis, septicemia) and three herds were asymptomatic, i.e., clinically normal. Twenty-eight isolates were Mycoplasma mycoides subspecies mycoides LC (large colony or caprine biotype), four were Mycoplasma capricolum subsp. capricolum and one was Mycoplasma cottewii. The isolated plasmids were linearized

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13

Maikai, Victor Ambrose, Beatty Viv Maikai, and Patricia Ishyaku Kobo. "In VitroEffect of Aqueous Extract and Fraction IV Portion ofXimenia americanaStem Bark onTrypanosoma congolenseDNA." Journal of Parasitology Research 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/904318.

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Trypanosomosis is a debilitating disease affecting mainly livestock and humans in tropical Africa. Chemically synthesized drugs and medicinal plants have been used in the treatment and control of this disease. In this study, thein vitroeffect of aqueous extracts and fraction IV extract ofXimenia americanastem bark onTrypanosoma congolenseDNA was investigated. The extracts were incubated with the parasitesin vitroat 300 mg/mL aqueous extract and 25 mg/mL fraction IV portion for 30, 60, and 120 mins. The DNA of the trypanosomes was isolated and digested using ECOR1 enzyme and subsequently PCR wa

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14

MCLEAN, DEREK J., NANCY KORN, BENILDA S. PEREZ, and RONALD J. THURSTON. "Isolation and Characterization of Mitochondria from Turkey Spermatozoa." Journal of Andrology 14, no. 6 (1993): 433–38. http://dx.doi.org/10.1002/j.1939-4640.1993.tb03257.x.

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ABSTRACT A procedure was developed to rapidly isolate functional, intact mitochondria from turkey spermatozoa. Semen was collected from turkeys, pooled, and centrifuged to remove spermiophages and other cells. The sperm cells were then mechanically disrupted with a Dounce homogenizer, sonicated, and centrifuged using a discontinuous Percoll gradient. Electron microscopy revealed morphologically intact mitochondria. The isolated mitochondria exhibited cytochrome oxidase activity, oxygen consumption, and were stained by rhodamine 123, a fluorescent stain specific for functional mitochondria in e

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15

Burr Furlong, N., Koenraad Marien, Ben Flook, and Jim White. "Characteristics of site variation among clones of the 340-base pair, tandemly repeated EcoR1 family of human DNA." Biochemical Genetics 24, no. 1-2 (1986): 71–78. http://dx.doi.org/10.1007/bf00502979.

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16

Liu, D., and P. R. Reeves. "Presence of different O antigen forms in three isolates of one clone of Escherichia coli." Genetics 138, no. 1 (1994): 7–10. http://dx.doi.org/10.1093/genetics/138.1.7.

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Abstract Escherichia coli strains ECOR2, ECOR3 and K-12 are very closely related in genotype as indicated by multilocus enzyme electrophoresis. We show that they have very different rfb regions indicating that recombination has occurred in this region, and we suggest that it may be associated with niche adaptation.

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Harrison, L. J. S., J. Delgado, and R. M. E. Parkhouse. "Differential diagnosis of Taenia saginata and Taenia solium with DNA probes." Parasitology 100, no. 3 (1990): 459–61. http://dx.doi.org/10.1017/s0031182000078768.

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SUMMARYA size selected genomic DNA library was constructed using DNA extracted from Taenia saginata. The DNA was digested using the restriction enzyme EcoR1 under star conditions and the 2–4 kbase fraction, selected following sucrose density-gradient separation, was cloned in the bacteriophage λgt 10. A panel of cestode DNAs including Taenia saginata, Taenia solium, Taenia taeniaeformis, Taenia crassiceps, Echinococcus granulosus and DNAs of bovine, porcine and human origin were used in conjunction with hybridization analysis to identify two recombinant bacteriophages. The first probe, designa

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18

Santos, Carlos Antônio Fernandes, and Viseldo Ribeiro de Oliveira. "Inter-relações genéticas entre espécies do gênero Spondias com base em marcadores AFLP." Revista Brasileira de Fruticultura 30, no. 3 (2008): 731–35. http://dx.doi.org/10.1590/s0100-29452008000300028.

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O objetivo deste trabalho foi construir um fenograma de trinta indivíduos de seis espécies de Spondias, amostradas em três diferentes locais, baseado em 120 marcadores AFLP das combinações de primers EcoR1/Mse1 para esclarecer as inter-relações entre as espécies, subsidiar a exploração das espécies como porta-enxerto e fornecer informações genômicas dessas espécies. O fenograma UPGMA do coeficiente de Jaccard apresentou boa definição, com valor co-fenético de 0,925 e localização externa de dois "outgroups" das espécies Mangifera indica e Schinopsis brasiliensis da família Anacardeaceae. Todos

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19

Vernet, Thierry, Ian J. McDonald, Dave R. Cameron, and Louis P. Visentin. "Stable maintenance in chemostat-grown Escherichia coli of pBR322 and pACYC184 by disruption of the tetracycline resistance gene*." Bioscience Reports 5, no. 1 (1985): 29–37. http://dx.doi.org/10.1007/bf01117438.

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Plasmid stability was studied in antibiotic-free chemo-stat cultures. Disruption, either by deletion or insertion, of the tetracycline resistance gene in the EcoRl/EcoRV region of the cloning vector pBR322 or in the HindIII]BamHl region of pACYCI84 yields plasmids markedly more stable than the parent plasmids. Thus, at least for these two instances, cloning of a partitioning (par) locus is not prerequisite for plasmid maintenance.

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20

Hussain, Mohd Hasnain, Suhaila Zainol, Nikson Fatt Ming Chong, and Awang Ahmad Sallehin Awang Husaini. "Characterisation of Klebsiella pneumoniae Xylanase and Increment of Its Activity in Heterologous Expression System." Borneo Journal of Resource Science and Technology 6, no. 1 (2016): 1–9. http://dx.doi.org/10.33736/bjrst.209.2016.

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A xylanase DNA sequence with a total length of 642 bp was previously isolated from a xylanolytic Klebsiellapneumoniae. Xylanase gene primers were designed with the addition of BamH1 and EcoR1 restriction enzymesites in order get a full xylanase gene that is in-frame with pSTAG expression vector. The isolated xylanasegene was amplified using the designed primers through PCR, then cloned and expressed in E. coli BL21 (DE3).In-silico characterization showed that the recombinant xylanase has a molecular weight of 23.9 kDa and a pI of9.32. The signal peptide cleavage site for the recombinant xylana

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21

Gaffar, Shabarni, Purba Upay, Iman Permana Maksum, et al. "Ekspresi Prethrombin-2 Manusia Recombinan dalamPichia pastoris dan Optimasi Kondisi Ekspresinya." Indonesian Journal of Pharmaceutical Science and Technology 4, no. 1 (2017): 27. http://dx.doi.org/10.15416/ijpst.v4i1.9766.

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Pretrombin merupakan prekursor dari trombin yang memiliki aktivitas proteolitik. Trombin merubah fibrinogen menjadi benang fibrin yang salah satu aplikasinya adalah dapat digunakan sebagai lem untuk menggantikan teknik jahitan pasca bedah. Aplikasi trombin untuk pembuatan lem fibrin menuntut diproduksinya trombin rekombinan. Tujuan dari penelitian ini adalah ekspresi gen pretrombin-2 (PT2) manusia rekombinan menggunakan sistem ekspresi Pichia pastoris. Gen pengode PT2 dirancang sesuaidengan kodon preferensi P. pastoris. Fragmen PT2 diamplifikasi dengan metoda PCR dengan penambahan sisi restrik

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22

Rajkumar, Gowri, Jagathpriya Weerasena, Rangika Silva, and Kumudu Fernando. "Genomic diversity of Sri Lankan New Improved Rice Varieties revealed by AFLP markers." International Journal of Applied Sciences and Biotechnology 4, no. 1 (2016): 32–38. http://dx.doi.org/10.3126/ijasbt.v4i1.13768.

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Genetic relationships among 28 new improved rice varieties were established using Amplified Fragment Length Polymorphism (AFLP) markers. Cultivars were analyzed with 10 EcoR1 and MseI primer combinations. A total of 517 fluorescent AFLP markers were generated and analyzed. Of these 480 fragments were polymorphic (92.84%) and 37 (7.16%) fragments were monomorphic. The Jaccard’s similarity indices (J) based on the AFLP profiles of the 28 varieties were computed and Unweighted Pair Group Method with Arithmetic mean (UPGMA) based dendrogram was constructed. The dendrogram separated varieties into

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KENNETT, CHARLES A., and BENJAMIN STARK. "Automated Ribotyping for the Identification and Characterization of Foodborne Clostridia." Journal of Food Protection 69, no. 12 (2006): 2970–75. http://dx.doi.org/10.4315/0362-028x-69.12.2970.

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The DuPont Qualicon RiboPrinter was employed to determine if automated ribotyping could be used to differentiate between and characterize various species of foodborne clostridia. EcoRI digests were used to ribotype 49 isolates that represented seven Clostridium species: C. aerotolerans, C. beirjerinckii, C. botulinum, C. butyricum, C. perfringens, C. putrificum, and C. sporogenes. EcoRV digests were also used to ribotype 17 C. botulinum isolates to determine if an alternate restriction enzyme was more suitable than was EcoRI for toxin typing. It was concluded that the RiboPrinter could be pote

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24

WOLFES, Heiner, Anja FLIESS, Fritz WINKLER, and Alfred PINGOUD. "Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases." European Journal of Biochemistry 159, no. 2 (1986): 267–73. http://dx.doi.org/10.1111/j.1432-1033.1986.tb09863.x.

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25

López-López, Karina, Frenyiline Jara-Tejada, and Juan Carlos Vaca-Vaca. "Caracterización molecular de un nuevo begomovirus aislado de cinco especies de arvenses colectadas en cultivos de tomate en Valle del Cauca." Acta Biológica Colombiana 24, no. 3 (2019): 528–37. http://dx.doi.org/10.15446/abc.v24n3.79366.

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Las arvenses son hospederos alternos de begomovirus (Geminiviridae), los cuales facilitan su persistencia y propagación a cultivos de interés agronómico, como el tomate. El objetivo de esta investigación fue obtener el genoma completo de un begomovirus bipartita encontrado en Amaranthus dubius, Rivina humilis, Rhynchosia minima, Desmodium sp. y Caesalpinia sp., las cuales fueron colectadas en cultivos de tomate en Ginebra y Cerrito, Valle del Cauca. El genoma del begomovirus fue obtenido utilizando amplificación por círculo rodante y digestión con las enzimas EcoRI y EcoRV, las cuáles cortan e

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26

Fatoumata, Kone-Kone, Aka Tano Cyrielle, Kone-Dakouri Yekayo, et al. "Effect of EcoR1 Polymorphism of the Apoprotein B100 Gene (Apo B 4154G>A) on Serum Lipid Profile in ART-Naive PLHIV." Biochemistry and Molecular Biology 6, no. 3 (2021): 48. http://dx.doi.org/10.11648/j.bmb.20210603.13.

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27

Siddiqui, Saika, and Jie Yuan. "Binding Characteristics Study of DNA based Aptamers for E. coli O157:H7." Molecules 26, no. 1 (2021): 204. http://dx.doi.org/10.3390/molecules26010204.

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E. coli O157:H7 is a pathogenic bacterium producing verotoxins that could lead to serious complications such as hemolytic uremia syndrome. Fast detection of such pathogens is important. For rapid detection, aptamers are quickly gaining traction as alternative biorecognition molecules besides conventional antibodies. Several DNA aptamers have been selected for E. coli O157:H7. Nonetheless, there has not been a comparative study of the binding characteristics of these aptamers. In this work, we present a comprehensive analysis of binding characteristics including binding affinity (Kd) and bindin

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28

Sarker, Md Mamun, Most Masuma Aktar, and Shah Md Ashraful Islam. "MOLECULAR CHARACTERIZATION AND PATHOGENICITY TEST ON CITRUS FRUITS BY GREEN MOLD AND BLUE MOLD." Journal of microbiology, biotechnology and food sciences 14, no. 2 (2024): e10642. http://dx.doi.org/10.55251/jmbfs.10642.

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Citrus fruits are essential for preventing various health conditions, including diabetes, neurological diseases, and cancer. Among the postharvest diseases affecting citrus fruits, Penicillium digitatum and Penicillium italicum are particularly significant. This study aimed to characterize Penicillium spp. isolated from three citrus varieties: orange (Citrus sinensis), small orange, and Malta. Pathogenicity tests confirmed that these Penicillium isolates were capable of infecting the tested citrus fruits. For molecular characterization, PCR amplification of the D1/D2 domain of 26S rDNA was per

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Zhu, X., K. P. Pruess, and T. O. Powers. "Mitochondrial DNA polymorphism in a black fly, Simulium vittatum (Diptera: Simuliidae)." Canadian Journal of Zoology 76, no. 3 (1998): 440–47. http://dx.doi.org/10.1139/z97-203.

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Mitochondrial DNA (mtDNA) was extracted from pooled field-collected samples representing six species of black flies (Cnephia dacotensis, Simulium bivittaum, S. johansenni, S. luggeri, S. piperi, S. vittatum) and compared by restriction fragment length polymorphism (RFLP) analysis. Morphospecies were molecularly distinct, with few shared restriction fragments. Eleven populations of S. vittatum were found that appeared to be homogeneous for a single mitochondrial haplotype. Ten other populations of S. vittatum showed extensive mitochondrial heterogeneity. In part, these samples contained mixture

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Fliess, Anja, Heiner Wolfes, Andre Rosenthal, et al. "Role of thymidine residues in DNA recognition by the EcoRI and EcoRV restriction endonucleases." Nucleic Acids Research 14, no. 8 (1986): 3463–74. http://dx.doi.org/10.1093/nar/14.8.3463.

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Vipond, I. Barry, Geoffrey S. Baldwin, and Stephen E. Halford. "Divalent Metal Ions at the Active Sites of the EcoRV and EcoRI Restriction Endonucleases." Biochemistry 34, no. 2 (1995): 697–704. http://dx.doi.org/10.1021/bi00002a037.

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Dion, Michel, and Claude Hamelin. "Cartographie physique de l'ADN du cytomégalovirus humain souche AD169." Canadian Journal of Microbiology 36, no. 5 (1990): 341–47. http://dx.doi.org/10.1139/m90-059.

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The whole human cytomegalovirus strain AD169 genome was cloned into plasmid pAT153 in the form of 25 HindIII fragments. Double and triple digestions of the recombinant plasmids with restriction endonucleases BamHI, BglII, ClaI, DraI, EcoRI, EcoRV, HindIII, HpaI, KpnI, PaeR7, PstI, SphI and XbaI yielded a detailed restriction map of human cytomegalovirus DNA. Knowing the exact position of numerous restriction sites in the viral DNA molecule, we have been able to examine very closely the heterologous region between the long and the short segments of the human cytomegalovirus genome. Key words: D

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Rasyid, Sri Anggarini, Muzuni, Satriani Syarif, et al. "Identification of ß-Catenin Gene as a Colorectal Cancer Controller in Mice <i>(Mus musculus)</i> Induced by Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS) Using PCR RFLP Method with EcoR1 and Hinif1." Journal of Biomimetics, Biomaterials and Biomedical Engineering 55 (March 28, 2022): 11–19. http://dx.doi.org/10.4028/p-o9k7r4.

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Colorectal cancer is cancer attacking the colon to the rectum. The pathophysiology of colorectal cancer occurs due to several causes, such as changes in normal colonic epithelial cells histopathologically through molecular processes. Another cause is that the adenomatous polyps become colorectal cancer due to the carcinogenesis process. Most colorectal cancers originate from adenocarcinomas. Colon cancer is characterized by the uncontrolled growth of cells in the epithelial lining of the large intestine. The type of this study was laboratory experimental research. The population was 2 mice tha

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Zahid, Maria. "Engineered Recombinant NDV-Fusion Protein and Its Polyclonal Antibodies Production." Pakistan Veterinary Journal 40, no. 04 (2020): 499–503. http://dx.doi.org/10.29261/pakvetj/2020.034.

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Antibodies have important role in biological research and diagnosis. Polyclonal antibodies can be produced against more than one epitope of an antigen. Newcastle disease (ND) is one of the most devastating diseases that considerably effects the global poultry industry. Newcastle disease virus (NDV) possess Fusion (F) protein for the attachment and pathogenicity. In the present study TA plasmid having F gene was restricted by EcoR1 and it was ligated into pET30a expression vector. The F gene ligated pET30a was transformed in BL21DE3 expression strain. After the expression of NDV F protein, it w

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35

AN, J., N. BEAUCHEMIN, J. ALBANESE, T. O. ABNEY, and A. K. SULLIVAN. "Use of a Rat cDNA Probe Specific for the Y Chromosome to Detect Male‐Derived Cells." Journal of Andrology 18, no. 3 (1997): 289–93. http://dx.doi.org/10.1002/j.1939-4640.1997.tb01921.x.

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ABSTRACT: A cDNA probe that exhibits specificity for the rat Y chromosome was generated by using a set of primers specific to the murine Sry gene, the sex‐determining region of the Y chromosome. A 459‐base pair (bp) DNA fragment was obtained by polymerase chain reaction (PCR) amplification from male, but not female, rat genomic DNA (EMBL Nucleotide Sequence Database accession number X89730). This DNA fragment was purified, cloned using a vector, and digested with EcoR1 to yield a 270‐bp DNA fragment. This 270‐bp cDNA fragment, when used as a probe in Southern blot analysis of rat DNA, was obse

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Rudd, Kenneth E. "Linkage Map of Escherichia coli K-12, Edition 10: The Physical Map." Microbiology and Molecular Biology Reviews 62, no. 3 (1998): 985–1019. http://dx.doi.org/10.1128/mmbr.62.3.985-1019.1998.

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SUMMARY A physical map, EcoMap10, of the now completely sequenced Escherichia coli chromosome is presented. Calculated genomic positions for the eight restriction enzymes BamHI, HindIII, EcoRI, EcoRV, BglI, KpnI, PstI, and PvuII are depicted. Both sequenced and unsequenced Kohara/Isono miniset clones are aligned to this calculated restriction map. DNA sequence searches identify the precise locations of insertion sequence elements and repetitive extragenic palindrome clusters. EcoGene10, a revised set of genes and functionally uncharacterized open reading frames (ORFs), is also depicted on EcoM

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Jeltsch, A., J. Alves, H. Wolfes, G. Maass, and A. Pingoud. "Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes." Proceedings of the National Academy of Sciences 90, no. 18 (1993): 8499–503. http://dx.doi.org/10.1073/pnas.90.18.8499.

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Setiarini, Nur Asih, Indriawati Indriawati, R. Susanti Susanti, and Endang Tri Margawati. "Design of the env-su Gene Coding for Surface Unit Protein as a Vaccine Candidate for Jembrana Disease Virus in In Silico." Jurnal Riset Biologi dan Aplikasinya 3, no. 1 (2021): 13. http://dx.doi.org/10.26740/jrba.v3n1.p13-22.

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Bali cattle are superior meat producers, but they are susceptible to Jembrana disease. The injection of the crude vaccine was considered ineffective, so the env-su gene was selected to express the Jembrana Surface Unit (JSU) protein as a candidate for the Jembrana vaccine. This study aimed to analyze the potential of the JSU protein as a candidate for the Jembrana vaccine and analyze the increase in the env-su gene expression which codon has been optimized in silico. Vaccine design was carried out through in silico including the selection of the SU protein sequences of the genus Lentivirus and

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Habib, Md Ahsan, Ashish Kumar Sarker, and Masaaki Tabata. "Interactions of DNA with H2TMPyP4+and Ru(II)TMPyP4+: Probable Lead Compounds for African Sleeping Sickness." Bangladesh Pharmaceutical Journal 17, no. 1 (2015): 79–85. http://dx.doi.org/10.3329/bpj.v17i1.22321.

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Interactions of DNA with free base porphyrin, tetrakis(1-methylpyridium-4-yl)porphyrin (H2TMPyP4+) and its metallo-derivative of ruthenium(II) have been investigated by UV-Vis, fluorescence and circular dichroism (CD) spectroscopy at 0.1 M NaCl, 7.5 pH and 25 °C. The results suggest that Ru(II)TMPyP4+ interacts with DNA via outside binding in self-stacking manner as indicated by UV-Vis data, a small red shift (?? =3 nm) and a minimal hypochromicity (10%) upon addition of DNA. CD spectra showed the presence of a new peak in the Soret region on addition of Ru(II)TMPyP4+ to DNA solution. On the o

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van Wamel, Willem J. B., Suzan H. M. Rooijakkers, Maartje Ruyken, Kok P. M. van Kessel та Jos A. G. van Strijp. "The Innate Immune Modulators Staphylococcal Complement Inhibitor and Chemotaxis Inhibitory Protein of Staphylococcus aureus Are Located on β-Hemolysin-Converting Bacteriophages". Journal of Bacteriology 188, № 4 (2006): 1310–15. http://dx.doi.org/10.1128/jb.188.4.1310-1315.2006.

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ABSTRACT Two newly discovered immune modulators, chemotaxis inhibitory protein of Staphylococcus aureus (CHIPS) and staphylococcal complement inhibitor (SCIN), cluster on the conserved 3′ end of β-hemolysin (hlb)-converting bacteriophages (βC-φs). Since these βC-φs also carry the genes for the immune evasion molecules staphylokinase (sak) and enterotoxin A (sea), this 8-kb region at the 3′ end of βC-φ represents an innate immune evasion cluster (IEC). By PCR and Southern analyses of 85 clinical Staphylococcus aureus strains and 5 classical laboratory strains, we show that 90% of S. aureus stra

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Chen, J., C. J. Chang, and R. L. Jarret. "Plasmids from Xylella fastidiosa strains." Canadian Journal of Microbiology 38, no. 9 (1992): 993–95. http://dx.doi.org/10.1139/m92-161.

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Plasmids were observed from three strains of Xylella fastidiosa. A restriction map of a plasmid, pXFPW1 of 1.3 kb, isolated from Xyl. fastidiosa strain PWT-22, causing periwinkle wilt disease, was constructed using EcoRI, HaeIII, HinfI, MspI, StyI, and TaqI fragments. No restriction site for BamHI, BglI, DraI, EcoRV, HindIII, PstI, SalI, NcoI, or XbaI was evident. Studies based on the intensity of ethidium bromide staining indicated that there are at least 60 copies of pXFPW1 per genome. pXFPW1 shares a high degree homology with two other plasmids from strains PD82-21 and mul1, the pathogens o

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EKATERINIADOU (Λ.Β. ΑΙΚΑΤΕΡΙΝΙΑΔΟΥ), L. V., E. BOUKOUVALA (Ε. ΜΠΟΥΚΟΥΒΑΛΑ), R. M. PAPI (Ρ.Μ. ΠΑΠΗ), A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ), V. GIANTZI (Β. ΓΙΑΝΤΖΗ), and D. A. KYRIAKIDIS (Δ.Α. ΚΥΡΙΑΚΙΔΗΣ). "Antibiotic resistance and distribution of SodCI, sopE, sefA genes among Salmonella enteric serotype Enteritidis isolates from poultry." Journal of the Hellenic Veterinary Medical Society 66, no. 2 (2018): 70. http://dx.doi.org/10.12681/jhvms.15589.

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Present work aimed to examine the antibiotic resistance of the Salmonella enterica serotype Enteritidis (SE) isolates from poultry, to study the plasmid-mediated ampicillin resistance and to detect and determine the distribution of sodCI, sopE and sefA genes. Thirty-five SE isolates from one-day chicks, layers and broilers were studied for susceptibility/resistance to sixteen antimicrobial agents; 23 (65.7%) of them showed resistance to ampicillin, 5 (14.3%) to ampicillin and tetracycline, 4 (11.45%) to tetracycline and 1 (2.9%) isolate showed multi-drug resistance. Ampicillin (AmpR) and ampic

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Pillay, Michael. "Variation of nuclear ribosomal RNA genes in Eragrostis tef (Zucc.) Trotter." Genome 40, no. 6 (1997): 815–21. http://dx.doi.org/10.1139/g97-805.

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Variation in the ribosomal RNA genes (rDNA) was examined to assess the genetic variability among 314 plants representing 28 accessions of Eragrostis tef, an important food crop. A restriction site map was constructed for the species by localization of the BamHI, BglII, DraI, EcoRI, EcoRV, NdeI, SacI, SpeI, XbaI, and XhoI sites. A comparison of this map with those of other grasses showed conservation of sites, especially in the coding region. However, a unique EcoRI site combined with a BamHI site in the 18S region may be of diagnostic value for the species. A BamHI fragment that spans the inte

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Yermekova, S., M. Orazgaliyeva, T. Goncharova, F. Rakhimbekova, and E. Serik. "p53 and EGFR gene mutations in malignant tumors of the lung." Oncologia i radiologia Kazakhstana 62, no. 4 (2021): 28–34. http://dx.doi.org/10.52532/2663-4864-2021-4-62-28-34.

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Relevance: Increased incidence of lung cancer globally and in Kazakhstan, lack of screening in hereditary cases, high mortality, and low survival of patients necessitate the study of the molecular genetic causes of the disease. At present, gene mutation studies for lung cancer diagnostics are expanding. However, many gene mutations revealed remain undercovered in the scientific literature, and there is not enough data on their prognostic and diagnostic value. The purpose of the study was to discover the specifics of the р53 gene mutations and reveal the EGFR exon 19 deletions and e

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Udupa, Kodetthoor B., and Chhanda Bose. "Identification of Erythroid Cell Stimulating Factor as a Unique Protein, Quiescin Q6: Role in Erythropoiesis." Blood 106, no. 11 (2005): 4270. http://dx.doi.org/10.1182/blood.v106.11.4270.4270.

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Abstract We demonstrated earlier the presence of an erythroid cell stimulating factor (ESF) in mouse serum that enhanced the erythropoietin-dependent erythroid cell proliferation in vitro. Employing a highly specific monoclonal antibody generated in our laboratory, we showed further that ESF had an essential role in normal erythropoiesis in vivo [Blood98, 296a, 2001, Exp Hematol.31, Supp. 1, 165, 2003]. In order to clone and express this protein, ESF was purified from mouse serum and on Western blotting using the monoclonal anti-ESF antibody the purified protein was stained as bands of ~62/64

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Faivre-Rampant, P., S. Jeandroz, F. Lefevre, M. Lemoine, M. Villar, and A. Berville. "Ribosomal DNA studies in poplars: Populus deltoides, P. nigra, P. trichocarpa, P. maximowiczii, and P. alba." Genome 35, no. 5 (1992): 733–40. http://dx.doi.org/10.1139/g92-113.

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The nuclear rDNA physical maps of Populus nigra, P. deltoides, P. trichocarpa, P. maximowiczii, and P. alba have been built up for the restriction enzymes EcoRI, EcoRV, BamHI, PstI, and SacI. There is no HindIII site. A large variability appeared between the species in the intergenic spacer mainly for EcoRI and PstI. For several clones of each species two to three major unit types coexist in the genome, while several minor units as well as length variant units or new unit types have been found. The variability between the species is due to different major unit types, while the variability betw

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Cholis, Nur. "Studi tentang polimorfisme ulat sutera F1 hibrid hasil persilangan ras Jepang dan ras Cina yang berasal dari pusat pembibitan Soppeng dan Temanggung dengan menggunakan enzim restriksi Pst1 dan EcoR1." Jurnal Ilmu-Ilmu Peternakan 25, no. 1 (2015): 61–65. http://dx.doi.org/10.21776/ub.jiip.2015.025.01.09.

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Yu, Chen, Gao Hongwei, Zhang Yanming, et al. "Analysis of the Bacterial Diversity Existing on Animal Hide and Wool: Development of a Preliminary PCR-Restriction Fragment Length Polymorphism Fingerprint Database for Identifying Isolates." Journal of AOAC INTERNATIONAL 95, no. 6 (2012): 1750–54. http://dx.doi.org/10.5740/jaoacint.11-482.

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Abstract Twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. The bacteria identified were Brevibacillus laterosporus, Leclercia adecarboxylata, Peptococcus niger, Bacillus circulans, Raoultella ornithinolytica, Bacillus subtilis, Bacillus cereus, Bacillus thermobacillus, Bacillus choshinensis, Bacillus sphaericus, Acinetobacter haemolyticus, Sphingomonas paucimobilis, Bacillus thuringiensis, Staphylococcus intermedius, Mycobacteria, Moraxella, Klebsiella pneumoniae, Ralstonia pickettii, St

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Hiller, David A., Amy M. Rodriguez, and John J. Perona. "Non-cognate Enzyme–DNA Complex: Structural and Kinetic Analysis of EcoRV Endonuclease Bound to the EcoRI Recognition Site GAATTC." Journal of Molecular Biology 354, no. 1 (2005): 121–36. http://dx.doi.org/10.1016/j.jmb.2005.09.046.

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50

Yermekova, S., M. Orazgaliyeva, T. Goncharova, F. Rakhimbekova, and E. Serik. "p53 and EGFR gene mutations in malignant tumors of the lung." Oncologia i radiologia Kazakhstana 62, no. 4 (2021): 28–34. http://dx.doi.org/10.52532/2521-6414-2021-4-62-28-34.

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Relevance: Increased incidence of lung cancer globally and in Kazakhstan, lack of screening in hereditary cases, high mortality, and low survival of patients necessitate the study of the molecular genetic causes of the disease. At present, gene mutation studies for lung cancer diagnostics are expanding. However, many gene mutations revealed remain undercovered in the scientific literature, and there is not enough data on their prognostic and diagnostic value. The purpose of the study was to discover the specifics of the р53 gene mutations a

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