Journal articles: 'EDTA titration'

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Bhavya sri Khagga, Srija Ghanukota, and Sumakanth Mogili. "Novel titrimetric method for the determination of rosuvastatin in pure form." World Journal of Biology Pharmacy and Health Sciences 13, no. 2 (2023): 001–4. http://dx.doi.org/10.30574/wjbphs.2023.13.2.0054.

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In this experiment assay of Rosuvastatin was performed by titrimetric method. The titration performed is a complexometric titration. The complexometric titration used is an easy, inexpensive volumetric titration method. This method is based on rection of calcium with a solution of disodium ethylenediaminotetraacetate (EDTA). Complex of calcium and EDTA is formed. Erichrome black Tis used as an indicator in this complexometric titration. At pH 12-13, Erichrome black T changes the color from pink to blue. The appearance blue color is determined to be the endpoint of this experiment. The titrations were carried out in triplicates and the average reading was taken to calculate the percentage assay. The percentage purity was found to be 103.05%

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Bhavya, sri Khagga, Ghanukota Srija, and Mogili Sumakanth. "Novel titrimetric method for the determination of rosuvastatin in pure form." World Journal of Biology Pharmacy and Health Sciences 13, no. 2 (2023): 001–4. https://doi.org/10.5281/zenodo.7948477.

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In this experiment assay of Rosuvastatin was performed by titrimetric method. The titration performed is a complexometric titration. The complexometric titration used is an easy, inexpensive volumetric titration method. This method is based on rection of calcium with a solution of disodium ethylenediaminotetraacetate (EDTA). Complex of calcium and EDTA is formed. Erichrome black Tis used as an indicator in this complexometric titration. At pH 12-13, Erichrome black T changes the color from pink to blue. The appearance blue color is determined to be the endpoint of this experiment. The titrations were carried out in triplicates and the average reading was taken to calculate the percentage assay. The percentage purity was found to be 103.05%

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Herlina, Herlina, and Muhammad Razali. "The redox titration of Fe (II) ions with K2Cr2O7 using a potentiometry method the effect of EDTA and SCN- ligands." Pharmaciana 14, no. 3 (2024): 276–88. https://doi.org/10.12928/pharmaciana.v14i3.28638.

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Complexometric titration is often used for determining the metal content, either through direct titration or back titration. This study aimed to investigate redox titration between Mohr salt solutions and potassium dichromate in an acidic atmosphere in the pH range 2. The results showed that the reaction proceeded effectively at pH 2, with Mohr's salt solution acting as titrant. Furthermore, experiments were conducted to compare the effectiveness of EDTA ligands and SCN- ligands in improving the sharpness of the Fe2+/ Cr2O72- redox titration curve at pH 2. Results show that EDTA ligands are more effective than SCN- ligands in improving the sharpness of the titration curve. However, it should be noted that the addition of EDTA ligands can shift the equivalent point volume earlier, so adjustments need to be made in redox titration analysis. Research has also shown that adding excess moles of EDTA to total Fe (II) ions can decrease redox potential in Fe2+/ Cr2O72- systems. These results provide additional insight into the use of EDTA ligands in redox titration analysis and their relevance to redox potential changes in the systems studied.

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Rajkovic, Milos. "Complexometric determination, Part II: Complexometric determination of Cu2+-ions." Chemical Industry 56, no. 10 (2002): 429–35. http://dx.doi.org/10.2298/hemind0210429r.

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A copper-selective electrode of the coated wire type based on sulphidized copper wire was applied successfully for determining Cu(II) ions by complexometric titration with the disodium salt of EDTA (complexon III). By the formation of internal complex compounds with the Cu(II) ion, the copper concentration in the solution decreases, and all this is followed by a change of potential of the indicator system Cu-DWISE (or Cu-EDWISE)/SCE. At the terminal point of titration, when all the Cu(II) ions are already utilized for the formation of the complex with EDTA, there occurs a steep rise of potential, thus enabling us, through the first or second derivative to note the quantity of copper that is present in the solution. Copper-selective electrode showed a responsivity towards titration with EDTA as a complexing agent, with the absence of "fatigue" due to a great number of repeated measurings. Errors occurring during quantitative measurements were more a characteristic of the overall procedure which involve, because of the impossibility of the complete absence of subjectivity, a constant error, and the reproducibility of the results confirmed this fact. The disodium salt of EDTA appeared as a very efficient titrant in all titrations and with various concentrations ot Cu(II) ions in the solution, with somewhat weaker response at lower concentrations in the solution.

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J, K. Mondal, Basak S., S. Mukherjee S., and Kundu Dipali. "Chelatometric titration of iron, zinc and aluminium involving masking and demasking with acetylacetone." Journal of Indian Chemical Society Vol. 79, Jan 2002 (2002): 92–94. https://doi.org/10.5281/zenodo.5843026.

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Analytical Chemistry Division, Central Glass & Ceramic Research Institute, Kolkata-700 032, India E-mail : dipali_kundu@hotmail.com Manuscript received 31 May 2000, revised 16 March 2001, accepted 12 May 2001 A stepwise complexometric method with EDTA has been developed for the determination of iron, zinc and aluminium in a single aliquot solution involving direct titration of iron(m)-acetylacetonate at pH 3.0 using acetylacetone as an auto-indicator. Zinc is determined in the same solution by direct titration with EDTA raising pH to 5.3 using xylenol orange as indicator, while aluminium being masked with acetylacetone at room temperature (25 ± 5°). However, aluminium is determined in the same solution indirectly with EDTA at pH 5.3 using xylenol orange as indicator after demasking aluminium from aluminium acetylacetonate at 90º and formation of AI-EDTA complex. Alternatively, aluminium may be determined by adding NaF and titrating released EDTA with zinc acetate solution. Interferences due to titanium, zirconium, manganese, nickel, cobalt, copper and chromium have been eliminated by complexing them with different ligands. The method has been standardised against synthetic solutions and applied to various enamel and frit samples.

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Risani Rusnel, Azizah, Dinda Putri Karim, Rahmida Marlini, and Siska Maharani. "Determination of CaCO3 Content in Egg Shell." Journal of Global Research Education 2, no. 2 (2025): 63–69. https://doi.org/10.62194/4y5hr711.

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The complexometry titration investigation has been conducting to ascertain the CaCO3 concentration of Egg. The complex substance EDTA will hold the calcium, and the point will be signaled by a change in the metallochromic indicator's color. Standardization EDTA : Pipette 25 mL of MgSO4.7H2O solution, 10 mL of buffer solution pH 10, a little amount of EBT indicator, and aqua DM into the titration flask. When you titrate with EDTA solution, the solution turns blue. Data processing has revealed that eggshell contains 0,025 grams of calcium and that the calcium content is 14,1%. Theoretically, eggshell is made up of crystals of CaCO3 (98,43%) based on the current mineral composition. Ca3(PO4)2 (0.75%) and MgCO3 (0.84%). Calcium carbonate is the main component of eggshell. When the titrant and titrate interact to create a complex molecule, the titration is known as a complexometric titration. A solution containing Ca2+ will form a complex with EDTA, according to the fundamental tenet of complexometry. CaCO3 was present in the sample (duck eggshell) in an amount of 0.025 grams, and a CaCO3 value of 15.7% was determined.

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Chen, Ling. "Macro Analysis Would Like to Drop Half a Drop of Solution." Advanced Materials Research 291-294 (July 2011): 3264–67. http://dx.doi.org/10.4028/www.scientific.net/amr.291-294.3264.

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By strong alkali (acid) titration strong acid (alkali), alkali (acid) titration mono-weak acid (alkali), precipitation titration and coordination titration to drop by half a drop, and settling the titration error specific terms, to illustrate the macro titration analysis would like to have drop half a drop of a solution. When strong alkali titrate strong acid or monobasic weak acid, there is no need to drop half a drop. As strong acid titrate strong alkali, there is must to drop half a drop as methyl orange for indicator. But phenolphthalein as indicator, there is doesn't need to drop half a drop. Strong alkali titrate monobasic weak acid, there is no need to drop half a drop. Eriochrome Black T for indicator titrate magnesium ion with EDTA, there is must to drop half a drop. Titration of zinc by EDTA, don't need to drop half a drop. Titration chloride ion with Moore method can not drop half a drop.

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Schnitzler, Egon, Márcio Lazzarotto, Marco Aurelio da Silva Carvalho-Filho, and Massao Ionashiro. "Thermoanalytical study of the complexes of 4-dimethylaminocynnamylidenepyruvate with manganese (II), cobalt (II), nickel (II), copper (II), zinc (II) and lead (II), in the solid state." Ecletica Quimica 27, no. 1 (2002): 41–50. http://dx.doi.org/10.26850/1678-4618eqj.v27.1.2002.p41-50.

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Solid state compounds M-4-DMCP, where 4-DMCP is 4-dimethylaminocinnamylidenepyruvate and M represents Mn (II), Co (II), Ni (II), Cu (II), Zn (II) and Pb (II) were prepared. These compounds were studied by thermoanalitycal techniques: thermogravimetry (TG), derivative thermogravimetry (DTG), differential scanning calorimetry (DSC), X-ray diffraction powder patterns and complexometric titration with EDTA. From the results obtained by the complexometric titration with EDTA, TG, DTG and DSC curves, was possible to establish the hydration degree, stoichiometry and thermal stability of the prepared compounds.

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Chen, Min, and R. Stephen Reid. "Solution speciation in the aqueous Na(I)–EDTA and K(I)–EDTA systems." Canadian Journal of Chemistry 71, no. 5 (1993): 763–68. http://dx.doi.org/10.1139/v93-100.

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Speciation in the aqueous sodium(I) – ethylenediaminetetraacetic acid and potassium(I) – ethylenediaminetetraacetic acid equilibrium systems was investigated by automated gravimetric potentiometric titration. It was found that in both cases, particularly below neutral pH values, speciation includes a previously uncharacterized diprotonated complex. All formation and acid dissociation constants were measured for the full systems. The validity of previous literature equilibrium constants, which are based on incomplete equilibrium models, is discussed.

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FUJIMURA, Kazuyoshi, and Tsuguo SAWADA. "Determination of tin in solders by EDTA titration." Bunseki kagaku 37, no. 7 (1988): 383–86. http://dx.doi.org/10.2116/bunsekikagaku.37.7_383.

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Lin, Kai Li, Zheng Wang, Jiang Chang, Hui Jun Zou, and Jian Xi Lu. "Fast and Simultaneous Determination of Calcium and Phosphorous in Calcium Phosphate Based Bioceramics by ICP-AES." Key Engineering Materials 361-363 (November 2007): 143–46. http://dx.doi.org/10.4028/www.scientific.net/kem.361-363.143.

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Calcium is generally determined by EDTA titration after separation of phosphate radicals, and the phosphorus is determined by weigh method. This traditional analytical process is time consuming and unfavourable for quality controlling. In contrast, the ICP-AES is simple and fast, and can simultaneously determine multielements. In this paper, the ICP-AES method was used to simultaneously determine calcium and phosphorous in calcium phosphate based bioceramics, and wavelengths of 317.933 nm and 213.618 nm were selected for the measuring of the calcium and phosphorus, respectively. The results obtained by ICP-AES are well consistent with the results determined by the traditional EDTA titration and weigh method, and suggest that the ICP-AES analysis is a simple, fast and accurate method for simultaneous determination of calcium and phosphorous in calcium phosphate based bioceramics.

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Zhang, Hua, Jacob P. Herman, Harvey Bolton, Zhicheng Zhang, Sue Clark, and Luying Xun. "Evidence that Bacterial ABC-Type Transporter Imports Free EDTA for Metabolism." Journal of Bacteriology 189, no. 22 (2007): 7991–97. http://dx.doi.org/10.1128/jb.01164-07.

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ABSTRACT EDTA, a common chelating agent, is becoming a major organic pollutant in the form of metal-EDTA complexes in surface waters, partly due to its recalcitrance to biodegradation. Even an EDTA-degrading bacterium, BNC1, does not degrade stable metal-EDTA complexes. In the present study, an ABC-type transporter was identified for possible uptake of EDTA because the transporter genes and the EDTA monooxygenase gene were expressed from a single operon in BNC1. The ABC-type transporter had a periplasmic-binding protein (EppA) that should confer the substrate specificity for the transporter; therefore, EppA was produced in Escherichia coli, purified, and characterized. EppA was shown to bind free EDTA with a dissociation constant as low as 25 nM by using isothermal titration calorimetry. When unstable metal-EDTA complexes, e.g., (Mg-EDTA)2−, were added to the EppA solution, binding was also observed. However, experimental data and theoretical analysis supported EppA binding only of free EDTA. When stable metal-EDTA complexes, e.g., (Cu-EDTA)2−, were titrated into the EppA solution, no binding was observed. Since EDTA monooxygenase in the cytoplasm uses some of the stable metal-EDTA complexes as substrates, we suggest that the lack of EppA binding and EDTA uptake are responsible for the failure of BNC1 cells to degrade the stable complexes.

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Xu, Dandan, Yuzhou Wang, Haiyu Wang, and Yuxi Zhao. "Investigation of the filling-overflow behavior of corrosion products in reinforced mortar." Journal of Asian Concrete Federation 9, no. 1 (2023): 43–50. http://dx.doi.org/10.18702/acf.2023.9.1.43.

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he investigation of the filling-overflow behavior of corrosion products in concrete is important for accurately predicting corrosion-induced concrete cover cracking. This paper utilizes the ethylene diamine tetra acetic acid (EDTA) titration method to quantitatively study the iron content in each zone of corroded reinforced mortar specimens. Based on this, the proportion and distribution of corrosion products are analyzed. Results show that approximately 50% of the corrosion products are transported to the mortar and corrosion products show a transport pattern to the surrounding low-concentration area in mortar. The direction of ion invasion on the mortar specimen does not exhibit a significant impact on the transport of corrosion products, while both the geometric size and quality of the mortar specimen affect the transport of corrosion products. Furthermore, the proposed method employs EDTA titration to quantify the filling and overflow of corrosion products, providing a valuable approach for further experimental research.

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Lopata, Jójárt, Surányi, et al. "Beyond Chelation: EDTA Tightly Binds Taq DNA Polymerase, MutT and dUTPase and Directly Inhibits dNTPase Activity." Biomolecules 9, no. 10 (2019): 621. http://dx.doi.org/10.3390/biom9100621.

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EDTA is commonly used as an efficient chelator of metal ion enzyme cofactors. It is highly soluble, optically inactive and does not interfere with most chemicals used in standard buffers making EDTA a common choice to generate metal-free conditions for biochemical and biophysical investigations. However, the controversy in the literature on metal-free enzyme activities achieved using EDTA or by other means called our attention to a putative effect of EDTA beyond chelation. Here, we show that EDTA competes for the nucleotide binding site of the nucleotide hydrolase dUTPase by developing an interaction network within the active site similar to that of the substrate. To achieve these findings, we applied kinetics and molecular docking techniques using two different dUTPases. Furthermore, we directly measured the binding of EDTA to dUTPases and to two other dNTPases, the Taq polymerase and MutT using isothermal titration calorimetry. EDTA binding proved to be exothermic and mainly enthalpy driven with a submicromolar dissociation constant considerably lower than that of the enzyme:substrate or the Mg:EDTA complexes. Control proteins, including an ATPase, did not interact with EDTA. Our findings indicate that EDTA may act as a selective inhibitor against dNTP hydrolyzing enzymes and urge the rethinking of the utilization of EDTA in enzymatic experiments.

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Belle-Oudry, Deirdre. "Quantitative Analysis of Sulfate in Water by Indirect EDTA Titration." Journal of Chemical Education 85, no. 9 (2008): 1269. http://dx.doi.org/10.1021/ed085p1269.

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YOSHIMURA, Chozo, Takayosi HUZINO, and Tadashi OHNISHI. "Conductometric titration of metal chelate with EDTA in nonaqueous media." Bunseki kagaku 37, no. 4 (1988): 169–73. http://dx.doi.org/10.2116/bunsekikagaku.37.4_169.

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Lewis, Kevin M., Chelsie L. Greene, Steven A. Sattler, Buhyun Youn, Luying Xun, and ChulHee Kang. "The Structural Basis of the Binding of Various Aminopolycarboxylates by the Periplasmic EDTA-Binding Protein EppA from Chelativorans sp. BNC1." International Journal of Molecular Sciences 21, no. 11 (2020): 3940. http://dx.doi.org/10.3390/ijms21113940.

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The widespread use of synthetic aminopolycarboxylates, such as ethylenediaminetetraacetate (EDTA), as chelating agents has led to their contamination in the environment as stable metal–chelate complexes. Microorganisms can transport free EDTA, but not metal–EDTA complexes, into cells for metabolism. An ABC-type transporter for free EDTA uptake in Chelativorans sp. BNC1 was investigated to understand the mechanism of the ligand selectivity. We solved the X-ray crystal structure of the periplasmic EDTA-binding protein (EppA) and analyzed its structure–function relations through isothermal titration calorimetry, site-directed mutagenesis, molecular docking, and quantum chemical analysis. EppA had high affinities for EDTA and other aminopolycarboxylates, which agrees with structural analysis, showing that its binding pocket could accommodate free aminopolycarboxylates. Further, key amino acid residues involved in the binding were identified. Our results suggest that EppA is a general binding protein for the uptake of free aminopolycarboxylates. This finding suggests that bacterial cells import free aminopolycarboxylates, explaining why stable metal–chelate complexes are resistant to degradation, as they are not transported into the cells for degradation.

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Li, Shixian, Kunpeng Zhou, Xinyin Xu, Yangyang Zhao, and Jing Li. "Application of Herbal Medicine in Cu2+ Removal from Water and Comparison of its Detection Methods." Scholars Journal of Agriculture and Veterinary Sciences 10, no. 05 (2023): 37–42. http://dx.doi.org/10.36347/sjavs.2023.v10i05.002.

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In order to utilize discarded herbal medicine to treat heavy metal pollution in water, various herbal medicines were selected to explore their abilities of metal ions removal. In this experiment, copper ion was used as targeted heavy metal ion and two different treatments of several herbal medicines were compared on the effect of the copper ion removal ability. At the evaluating stage, four metal ion detection methods were employed and analyzed in this herbal medicine Cu2+ removal application. The results showed that directly soaking herbal medicine in the copper aqueous solution was more effective and easier to operate. Iodometry method and EDTA complexometric titration with xylenol orange as indicator were accurate and time-saving in the measurement of copper removal rate. In addition, the EDTA complexometric titration is also applicable in the detection of other metal ions. This experiment provided a basis for the implementation and application of herbal medicine in the deposition of metal ions in polluted water.

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Tyagi, Somya, Sonali Taneja, Kandasamy Nagarajan, and Divya Chowdhary. "Comparative evaluation of free available chlorine in sodium hypochlorite solutions admixed with novel chelating agents." Endodontology 37, no. 2 (2025): 188–93. https://doi.org/10.4103/endo.endo_219_24.

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Aim: This study evaluated the effects of ethylenediaminetetraacetic acid (EDTA), etidronic acid (HEBP), peracetic acid (PAA), phytic acid (IP6), and glycolic acid (AHA) on the free available chlorine (FAC) content of sodium hypochlorite (NaOCl) solution when mixed in varying proportions. Methods: Twenty-five milliliter aliquots of 5.25% NaOCl were admixed in ratios of 90:10, 80:20, and 50:50 with secondary irrigation solutions: (a) 17% EDTA, (b) 9% HEBP, (c) 1% PAA, (d) 1% IP6, and (e) 10% AHA. Iodometric titration was done to evaluate the change in FAC. Statistical differences between means were determined using a post hoc Tukey’s analysis test after an analysis of variance. Results: On titration, the mean FAC value of NaOCl at baseline was 2.02 g%. On mixing NaOCl with EDTA and HEBP, the FAC decreased in all three proportions. On mixing NaOCl and PAA, the FAC increased on increasing dilution in all three proportions. On mixing NaOCl and IP6, the FAC decreased only in 80:20 dilutions. On mixing NaOCl and AHA, the FAC increased on increasing dilution in all three proportions. Conclusion: The FAC on the combination of NaOCl with AHA, PAA, and IP6 remained unaltered or decreased marginally, suggesting that the antimicrobial properties of NaOCl might be preserved.

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Mach, Vladimír, Stanislav Kotrlý, and Václav Říha. "Standardization of dilute solutions of EDTA by photometric titration with standard tetraamminecopper(II) titrant." Collection of Czechoslovak Chemical Communications 56, no. 9 (1991): 1850–55. http://dx.doi.org/10.1135/cccc19911850.

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Direct photometric titration (without an added indicator) using a standard ammoniacal solution of copper(II) prepared from pure copper metal asa primary standard is recommended as a reliable method for the standardization of Na2H2(edta) in 10-3 -5 . 10-4M solutions. The technique of photometric microtitration (720 nm, 50 mm cell) with an increased precision of transmittance reading (s ~10-2%, n > 7) and in optimum medium of pH 4.7 ± 0.4 (acetate buffer) can yield precise results in assays of EDTA (sr ⪬ 10-1%, n = 6-9).

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Pradeep, Sharma, and Rajpal Ratna. "Analytical characterisation of soil samples polluted by industrial effluents for exchangeable cations." Journal of India Chemical Society Vol 81, Sep 2004 (2004): 797–98. https://doi.org/10.5281/zenodo.5833322.

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Electroanalytical Laboratory, Department of Chemistry, J. N. V. University, Jodhpur-342 003, India Manuscript received 25 November 2003. accepted 17 March 2004 The prominent exchangeable cations Ca-Mg and Na-K have been determined in soils collected near the vicinity of textile industries for the purpose of pollution monitoring using classical methodology of EDTA complexation and flame photometry.

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Zhang, Zhuang, Feng Xie, Wei Wang, and Yun-Long Bai. "A Novel Quantitative Analysis Method for Lead Components in Waste Lead Paste." Metals 13, no. 9 (2023): 1517. http://dx.doi.org/10.3390/met13091517.

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In this study, a method for determining the lead components in waste lead paste was proposed, using simulated and spent lead paste as research objects. To compare the effectiveness of different determining methods, we selected three methods for comparison and investigated the reasons for measurement deviation. The results indicate that the measurement deviation in the current method primarily stems from the following three factors: (1) Pb is soluble in an acetic acid solution under certain conditions; (2) Pb and PbO2 undergo redox reactions; and (3) hydrogen peroxide can undergo redox reactions with Pb. It is feasible to determine the lead content using the kinetic rules of Pb and PbO2 in the acetic acid-hydrogen peroxide system. The method of determination proposed in this paper is as follows. Firstly, lead dioxide is dissolved in hydrogen peroxide under acidic conditions. Subsequently, the concentration of lead dioxide is determined, and the quantity of hydrogen peroxide consumed is recorded. Then, a new sample is taken, and the lead oxide is dissolved in an acetic acid solution. The concentration of lead oxide is determined using the EDTA·2Na titration method. The residue of lead sulfate in the filtrate is dissolved in a sodium chloride solution, and its concentration is determined using the EDTA·2Na titration method. Based on the previously recorded volume of hydrogen peroxide, the remaining lead dioxide in the residue is dissolved in a mixture of acetic acid and hydrogen peroxide. The remaining lead dioxide is then removed from the new sample employing kinetic principles. Finally, the residual metallic lead in the sample is dissolved in a nitric acid solution, and its concentration is determined using the EDTA·2Na titration method.

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Umerska, Anita, Magnus Strandh, Viviane Cassisa, Nada Matougui, Matthieu Eveillard, and Patrick Saulnier. "Synergistic Effect of Combinations Containing EDTA and the Antimicrobial Peptide AA230, an Arenicin-3 Derivative, on Gram-Negative Bacteria." Biomolecules 8, no. 4 (2018): 122. http://dx.doi.org/10.3390/biom8040122.

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The worldwide occurrence of resistance to standard antibiotics and lack of new antibacterial drugs demand new strategies to treat complicated infections. Hence, the aim of this study was to examine the antibacterial activities of an antimicrobial peptide, arenicin-3 derivative AA230, and ethylenediaminetetraacetic acid (EDTA) as well as the two compounds in combination against Gram-negative bacteria. AA230 showed strong antibacterial activity against all of the studied standard strains and clinical isolates, with minimum inhibitory concentrations ranging between 1 µg/mL and 8 µg/mL. AA230 exhibited a bactericidal mode of action. EDTA inhibited the growth of Acinetobacter baumannii at 500–1000 µg/mL. Strains of Acinetobacter baumannii were found to be more susceptible to EDTA than Pseudomonas aeruginosa or Escherichia coli. The antibacterial effects of both AA230 and EDTA were independent of the antibiotic resistance patterns. Indifference to synergistic activity was observed for AA230 and EDTA combinations using checkerboard titration. In time-kill studies, a substantial synergistic interaction between AA230 and EDTA was detected against all of the tested strains. The addition of EDTA enabled a 2–4-fold decrease in the AA230 dose. In conclusion, AA230 could have potential applications in the treatment of infections caused by Gram-negative organisms, and its effect can be potentiated by EDTA.

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Benjamin Uchenna Modozie, Nkechi Helen Okoye, Innocent Oghale Ajawobu, et al. "Use of Ipomoea involucrata B. flower as metallochromic indicator for complexometric titrations." GSC Biological and Pharmaceutical Sciences 25, no. 1 (2023): 095–100. http://dx.doi.org/10.30574/gscbps.2023.25.1.0408.

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The present work highlights the use of Ipomoea involucrate B. flower extract as a complexometric indicator in complex formation between the EDTA and the following metal solutions (Cu2,+ Ba2,+ Hg2+, Fe3+, and Bi3+). Ipomoea involucrate is a species of the Ipomoea genus, belonging to the family Convolvulaceae. This natural indicator was extracted in 2M HCl at 100 0C for 30-40 minutes, cooled and the extract filtered and extracted with ethyl acetate and the pigment extracted with amyl alcohol which was separated and concentrated over a water bath. The flower extract was screened for its use as an metallochromic indicator at different pHs in metal solutions of Cu2,+ Ba2,+ Hg2+, Fe3+, and Bi3+.This natural indicator from the results obtained gave sharp intense color change at the equivalence point of complex formation between the metals and the EDTA at both acidic and alkaline pHs in the metal solution of Cu2+ and Fe3+ and that with different amount of EDTA used in the titration, which increased as the pH value increased, while the ipomoea indicator gave sharp intense color change at the equivalence point of complex formation between the metals and the EDTA at alkaline pHs in the metal solution of Ba2+, Hg2+ and Bi3+ at pH > 10. Natural flower indicators could take the place of the synthetic metallochromic indicators currently used in conventional laboratories because these flower extracts have excellent performance with sharp and intense color change in end points during the complexometric titrations and simple, cost-effective and environmentally friendly extraction methods.

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COSTA, Luiz Henrique Medeiros da, Francisco José Santos LIMA, and Ademir Oliveira da SILVA. "SYNTHESIS, COMPLEXOMETRY, THERMAL ANALYSIS AND MOLAR CONDUCTANCE OF LANTHANUM CHLORIDE, NEODYMIUM AND ERBIUM HYDRATED." Periódico Tchê Química 10, no. 19 (2013): 38–45. http://dx.doi.org/10.52571/ptq.v10.n19.2013.38_periodico19_pgs_38_45.pdf.

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The lanthanum, neodymium and erbium chlorides were prepared from your respective starting oxides in aqueous suspension with slow addition of hydrochloric acid and later placed in desiccator. The compositions were characterized through of the complexometric titrations with EDTA, thermogravimetry (TG) and molar conductance. The TG curves were accomplished in synthetic air and we verified your thermal stabilities, and the process of thermal decomposition. The results obtained by the titration and for TG they made possible to establish the stoichiometry of the compounds that came as: LaCl3.7H2O, NdCl3.6H2O e ErCl3.7H2O. The molar conductance, in water, informed us a type of electrolyte 1:3, evidencing that the ions chlorides is not coordinated to the lanthanides and they are out of the coordination sphere in these complex salts.

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Deng, Chan Gai, Li Fu Liao, and Wei Long. "Simultaneous Determination of Copper, Lead and Zinc in Alloy by Potentiometric Titration Coupled with Multivariate Calibration Using a Flow Injection Technique." Advanced Materials Research 785-786 (September 2013): 1353–58. http://dx.doi.org/10.4028/www.scientific.net/amr.785-786.1353.

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A new potentiometric titration method coupled with multivariate calibration using a flow injection technique for the simultaneous determination of multi-components in alloy has been developed. the titrant is a mixture of EDTA and sodium fluoride, both mercury film electrode and fluorinion selective electrode are inserted in the flow cell. In a process of titration, both the potentiometric of mercury ion and the mixed proportion of reactants at each titration point can be obtained simultaneously from the two electrodes, and then the concentration of each component in the mixture can be obtained from the titration curves by multivariate calibration method. The method is free of volumetric and time measurements, and reduces the consumption of reagents and samples. The method is also very quick in analysis rapidity. The method has been applied for the simultaneous determination of copper, lead and zinc in alloy samples with the relative standard deviation of 0.18%~0.45% and the recovery of 99.5%~101.6%.

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YOSHIMURA, Chozo, and Yoshinobu YAGITA. "Conductometric titration of metal-cupferron chelate with EDTA in nonaqueous media." Bunseki kagaku 38, no. 6 (1989): 267–71. http://dx.doi.org/10.2116/bunsekikagaku.38.6_267.

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Jayachandran, Kavitha, J. S. Gamare, P. R. Nair, Mary Xavier, and Suresh K. Aggarwal. "A novel biamperometric methodology for thorium determination by EDTA complexometric titration." Radiochimica Acta 100, no. 5 (2012): 311–14. http://dx.doi.org/10.1524/ract.2012.1920.

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N., Gopalakrishna Bhat, and Narayana B. "Indirect complexometric determination of thorium(IV) using tartaric acid as masking agent." Journal of Indian Chemical Society Vol. 89, Mar 2012 (2012): 427–28. https://doi.org/10.5281/zenodo.5760339.

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Department of Chemistry, Srinivas Institute of Technology, Valachil, Mangalore-574 143, Karnataka, India E-mail : shreedakumar@yahoo.co.in Manuscript received 05 October 2009, revised 07 July 2011, accepted 21 July 2011 A complexometric method for the determination of thorium(IV) in presence of other metal ions based on the selective masking ability of tartaric acid towards thorium is described. Thorium(IV) present in a given sample solution is first complexed with a known excess of EDTA and the surplus EDTA is titrated against bismuth nitrate solution at pH 2-3 using xylenol orange as indicator. A known excess of tartaric acid (10%) is then added and the EDTA released from the Th-EDTA complex is titrated against standard bismuth nitrate solution. Reproducible accurate results are obtained for 9.36 to 46.8 mg of thorium with relative errors ±0.21% and standard deviations s; 0.06 mg. The interference of various ions were studied. The method is applied for the determination of thorium in alloy solutions.

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Rani, Geeta, and Sanjay Singh. "Determination of Thallium(I) by Hybrid Mesoporous Silica (SBA-15) Modified Electrode." International Journal of Electrochemistry 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/9075651.

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Chemically modified mesoporous silica material (SBA-15) was used for the construction of Tl(I) selective carbon paste electrode. The best response was found with the electrode containing 10% modifier as electrode material. The electrode has a lower detection limit of 6.0 × 10−9 M in a working concentration range of 1.0 × 10−8–1.0 × 10−1 M. The selectivity coefficient calculated by match potential method (MPM) shows the high selectivity of electrode towards Tl(I) over other tested ions. The electrode was successfully applied as an indicator electrode for the titration of 0.01 M TlNO3solution with standards EDTA solution and for sequential titration of mixture of different anions.

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Barbosa, A. F. F., J. D. Cunha, A. G. Silva, F. J. S. Lima, M. K. S. Batista, and A. O. Silva. "1.10 – PHENANTHROLINE COMPLEXES OF THE RARE EARTH NITRATE: SYNTHESIS AND CHARACTERIZATION." Periódico Tchê Química 04, no. 2 (2005): 46–50. http://dx.doi.org/10.52571/ptq.v2.n04.2005.agosto/4_pgs_46_50.pdf.

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The interaction of lanthanide nitrates (Ln–Nd, Sm, Dy and Er) and 1.10–phenantroline were studied and complexes of the general formulae Ln(NO3)3(C12H8N2)2 or Ln(NO3)3(o-fen)2. The complexes were characterized by elemental analyses (CHN), titration with EDTA, IR spectra and thermogravimetric analysis. X-ray powder patterns further indicated that the complexes are isomorphous having the same d -interplanar spacing.

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Vasilyeva, Marina S., Vladimir S. Rudnev, Irina V. Lukiyanchuk, N. E. Zabudskaya, and I. V. Chasovnikov. "Sn-Containing Oxide Coatings: Formation, Composition, Electroanalytical and Catalytic Properties." Key Engineering Materials 806 (June 2019): 70–75. http://dx.doi.org/10.4028/www.scientific.net/kem.806.70.

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Sn-containing oxide coatings were prepared via plasma electrolytic oxidation (PEO) of Ti plate in the electrolytes with [SnII-EDTA]2- complex anions or SnO2 particles in the anode and anodic-cathodic modes. The coatings formed in electrolyte with SnO2 particles stabilized by SAS contain SnO2 and Sn0. In the electrolyte with [Sn-EDTA]2- complex anions, the SnO2-containing coatings were formed in the anodic mode while Sn0-containing ones were obtained in the anodic-cathodic mode. SnO2-containing structures formed in the electrolytes with [Sn-EDTA]2- anions are shown to be active in catalytic oxidation of CO into CO2 at temperatures above 350 °C. They can be the basis for the preparation of both carriers of catalytically active compounds and catalysts for redox reactions. Potentiometric tests showed that the Sn-containing PEO layers on titanium exhibit the most characteristic pH function for the metal oxide electrodes in the direct potentiometry and acid-base titration.

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Shirey, Steven B., Jay L. Banner, and Gilbert N. Hanson. "Cation-exchange column calibration for Sr and the REE by Edta Titration." Chemical Geology: Isotope Geoscience section 65, no. 2 (1987): 183–87. http://dx.doi.org/10.1016/0168-9622(87)90072-8.

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Zhang, Fuhai, Lei Zhang, Liguo Yang, Tugen Feng, Lei Chen, and Xiaochun Zhong. "The Cement Content Measurement of Cement Mixing Piles with EDTA Titration Method." KSCE Journal of Civil Engineering 22, no. 11 (2018): 4306–15. http://dx.doi.org/10.1007/s12205-018-1215-z.

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Kim, Jeewoong, and C. Vipulanandan. "Effect of pH, sulfate and sodium on the EDTA titration of calcium." Cement and Concrete Research 33, no. 5 (2003): 621–27. http://dx.doi.org/10.1016/s0008-8846(02)01043-8.

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YOSHIMURA, Chozo, and Tadashi OHNISHI. "Conductometric titration of metal-8-quinolinol chelate with EDTA in nonaqueous media." Bunseki kagaku 35, no. 10 (1986): 839–43. http://dx.doi.org/10.2116/bunsekikagaku.35.10_839.

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Zulfisa Zulfisa and Rini Lestari. "Penetapan Kadar Kalsium Pada Daun Krokot (Portulaca Oleracea L.) Secara Kompleksometri." Journal Pharma Saintika 6, no. 1 (2022): 01–07. http://dx.doi.org/10.51225/jps.v6i1.15.

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Research on the determination of calcium levels in purslane (Portulaca oleracea L.) leaves has been carried out using the indirect complexometric titration method. Fresh purslane leaves were extracted using 3N HC1, dissolved calcium was added to an excess of 0.05 M NazEDTA solution and the excess Na EDTA was titrated with 0.05 M MgSO4 solution using an EBT indicator. Obtained calcium levels in fresh purslane leaves of 0.1503% w/w.

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Kirakosyan, Virab, and Andranik Davinyan. "Method Validation of Compleximetric Titration for Determination of Iron in Iron Containing Drugs." Der Pharma Chemica 13, no. 5 (2021): 6. https://doi.org/10.5281/zenodo.11072367.

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A simple and inexpensive titrimetric method was developed and validated according to International Conference on Harmonization (ICH) and United State Pharmacopoeia (USP). The method is based on complex formation between ferric (Fe3+) ions and disodium edetate (EDTA) in strong acidic media.The endpoint of titration was determined visually. Sulfosalicylic acid was used as an indicator (color change: from dark pink to light yellow at pH = 2). The method was validated regarding to linearity, precision, accuracy and robustness giving good results.

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Benjamin, Uchenna Modozie, Helen Okoye Nkechi, Oghale Ajawobu Innocent, et al. "Use of Ipomoea involucrata B. flower as metallochromic indicator for complexometric titrations." GSC Biological and Pharmaceutical Sciences 25, no. 1 (2023): 095–100. https://doi.org/10.5281/zenodo.10575014.

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The present work highlights the use of Ipomoea involucrate B. flower extract as a complexometric indicator in complex formation between the EDTA and the following metal solutions (Cu2,+ Ba2,+ Hg2+, Fe3+, and Bi3+). Ipomoea involucrate is a species of the Ipomoea genus, belonging to the family Convolvulaceae. This natural indicator was extracted in 2M HCl at 100 0C for 30-40 minutes, cooled and the extract filtered and extracted with ethyl acetate and the pigment extracted with amyl alcohol which was separated and concentrated over a water bath. The flower extract was screened for its use as an metallochromic indicator at different pHs in metal solutions of Cu2,+ Ba2,+ Hg2+, Fe3+, and Bi3+.This natural indicator from the results obtained gave sharp intense color change at the equivalence point of complex formation between the metals and the EDTA at both acidic and alkaline pHs in the metal solution of Cu2+ and Fe3+ and that with different amount of EDTA used in the titration, which increased as the pH value increased, while the ipomoea indicator gave sharp intense color change at the equivalence point of complex formation between the metals and the EDTA at alkaline pHs in the metal solution of Ba2+, Hg2+ and Bi3+ at pH > 10. Natural flower indicators could take the place of the synthetic metallochromic indicators currently used in conventional laboratories because these flower extracts have excellent performance with sharp and intense color change in end points during the complexometric titrations and simple, cost-effective and environmentally friendly extraction methods.

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Oliveira, Juliana Santos, Walter Raucci Neto, Natália Spadine de Faria, Fernanda Silva Fernandes, Carlos Eduardo Saraiva Miranda, and Fuad Jacob Abi Rached-Junior. "Quantitative Assessment of Root Canal Roughness with Calcium-Based Hypochlorite Irrigants by 3D CLSM." Brazilian Dental Journal 25, no. 5 (2014): 409–15. http://dx.doi.org/10.1590/0103-6440201302433.

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Chemical solutions play important roles in endodontic treatment and promote ultrastructural changes in dentin surface. The aim of this study was to quantify root canal roughness at different concentrations of calcium hypochlorite (Ca(OCl)2) and sodium hypochlorite (NaOCl) by confocal laser scanning microscopy (CLSM). Fifty-two human mandibular premolars were sectioned and randomly organized into thirteen groups (n=8): saline (control); 1%, 2.5% and 5% NaOCl; 1%, 2.5% and 5% Ca(OCl)2; the hypochlorite groups were further divided into with or without EDTA. The chlorine concentrations of the different solutions were measured by iodine titration (%). The superficial roughness (Sa) was quantified by CLSM. Ca(OCl)2 presented substantial decrease in chlorine concentration that differed from the package indication, but without compromising the dentin ultrastructure changes. There were no significant differences in dentin roughness between Ca(OCl)2 or NaOCl at all studied concentrations. The combination with EDTA provided similar roughness values among the solutions (p>0.05). The 5% Ca(OCl)2 and NaOCl solutions significantly increased dentin roughness and did not differ from the EDTA association (p>0.05). Ca(OCl)2 promoted similar dentin roughness as the NaOCl at the same concentrations and combined with EDTA. It may be concluded that Ca(OCl)2 modified the root canal dentin roughness similarly to NaOCl, at the same concentrations and EDTA combinations used in this study. Ca(OCl)2 and NaOCl, both at 5%, significantly altered dentin roughness, overcoming EDTA association, thus Ca(OCl)2 concentrations ranging from 1% to 2.5% may be suitable solutions for root canal irrigation protocols.

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Kozak, Joanna, Justyna Paluch, Marek Kozak, Marta Duracz, Marcin Wieczorek, and Paweł Kościelniak. "Novel Approach to Automated Flow Titration for the Determination of Fe(III)." Molecules 25, no. 7 (2020): 1533. http://dx.doi.org/10.3390/molecules25071533.

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A novel approach to automated flow titration with spectrophotometric detection for the determination of Fe(III) is presented. The approach is based on the possibility of strict and simultaneous control of the flow rates of sample and titrant streams over time. It consists of creating different but precisely defined concentration gradients of titrant and analyte in each successively formed monosegments, and is based on using the calculated titrant dilution factor. The procedure was verified by complexometric titration of Fe(III) in the form of a complex with sulfosalicylic acid, using EDTA as a titrant. Fe(III) and Fe(II) (after oxidation to Fe(III) with the use of H2O2) were determined with good precision (CV lower than 1.7%, n = 6) and accuracy ( | RE | lower than 3.3%). The approach was applied to determine Fe(III) and Fe(II) in artesian water samples. Results of determinations were consistent with values obtained using the ICP–OES reference method. Using the procedure, it was possible to perform titration in 6 min for a wide range of analyte concentrations, using 2.4 mL of both sample and titrant.

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Nur Khadijah Anasyah, Nurfadillah Nurfadillah, Dian Fadillah, et al. "Penetapan Kadar Kalsium Laktat dengan Menggunakan Metode Titrasi Kompleksometri." OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan 3, no. 1 (2025): 269–75. https://doi.org/10.61132/obat.v3i1.1033.

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This research aims to determine calcium lactate levels using the complexometric titration method, which involves the formation of a complex between metal ions and ligands, specifically using EDTA (Ethylenediaminetetraacetate) as a titrant. The sample was dissolved in distilled water, the murexide indicator was added, and titrated with complexon III solution until the color changed from orange to violet as a marker for the end point of the titration. Data analysis shows that the percentage of calcium lactate levels in replication (1) is 93.32%, replication (2) 101.60%, replication (3) 95.93%, replication (4) 103.78%, replication (5) 109.02%, replication (6) 87.87%, replication (7) 95.93% and replication (8) 100.95%, and the percentage obtained the average purity is 98.55%. However, replications 5 and 6 did not comply with the standards of the Indonesian Pharmacopoeia VI edition (94.0–106.0%). This research shows that the complexometric titration method is effective in determining calcium lactate levels with good accuracy, as long as it is carried out with appropriate procedures and strict control of the analysis conditions.

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Krishna Prasad, K. M. M., and Syed Raheem. "Evaluation of colour changes of indicators in the titration of cadmium with EDTA." Analytica Chimica Acta 264, no. 1 (1992): 137–40. http://dx.doi.org/10.1016/0003-2670(92)85308-s.

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Ijaz, Saadia, Farheen Ansari, Muhammad Nawaz, et al. "Genomic Insights into and In Vitro Evaluation of Antimicrobial Combination Therapies for Carbapenem-Resistant Acinetobacter baumannii." Medicina 60, no. 7 (2024): 1086. http://dx.doi.org/10.3390/medicina60071086.

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Background and Objectives: Acinetobacter baumannii (A. baumannii), particularly carbapenem-resistant A. baumannii (CRAB), represents a grave concern in healthcare settings and is associated with high mortality. This study aimed to conduct molecular, mutational, and phylogenetic analyses of specific genes in CRAB and evaluate the synergistic effects of selected antimicrobial combinations. Materials and Methods: Phenotypic characterization was performed on six CRAB strains by using the Modified Hodge Test (MHT) and IMP-EDTA Double-Disc Synergy Test (IMP-EDTA DDST). Carbapenemase- and metallo-beta-lactamase-encoding genes were amplified by using Polymerase Chain Reaction. Phylogenetic analysis using the MEGA 11 tool was used to determine the evolutionary relatedness of these genes. Mutational analysis was performed by using I-Mutant, MUPro, and PHD-SNP bioinformatics tools to predict mutations in the carbapenemase-encoding genes. Microdilution checkerboard titration assessed the synergistic effects of antimicrobial combinations (azithromycin–meropenem, rifampicin–meropenem, meropenem–colistin, and azithromycin–colistin) on these CRAB isolates. Results: The phenotypic characterization of six CRAB isolates revealed positive results for MHT and IMP-EDTA DDST. The molecular characterization revealed that carbapenemase- and MBL-encoding genes were present in all isolates with varying frequencies, including blaOXA-51 (100%) and blaIMP (0%). The sequence analysis revealed high evolutionary relatedness to sequences in the NCBI database. The mutational analysis identified 16 mutations, of which 1 mutation (P116L) in the blaOXA-58 gene predicted a change in the protein product, potentially contributing to carbapenem resistance. The checkerboard titration method did not reveal any synergism among the tested antimicrobial combinations against CRAB. Conclusion: This study’s findings underscore the significant challenges posed by CRAB isolates harboring multiple resistant genes in treatment. This highlights the urgent need for novel antimicrobial agents, a crucial step towards reducing mortality rates not only in Pakistan but also globally.

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Wang, Jianhong, Jinyong Wang, Quanlei Wang, et al. "The influence of mixing technology on the uniformity and strength of cement stabilized macadam mixture." Vibroengineering PROCEDIA 43 (June 13, 2022): 1–6. http://dx.doi.org/10.21595/vp.2022.22623.

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A comparison between the two strategies: secondary traditional mixing technology and secondary vibratory mixing technology is proposed based on aggregate sieving, cement EDTA titration and unconfined compressive strength tests to explore the influence of the uniformity and strength of cement stabilized macadam mixture in the paper. The results show that when the secondary vibratory mixing technology is adopted: (1) the standard deviations and coefficients of variation of the passing rates of key sieves of 19 mm, 13.2 mm, 9.5 mm, 4.75 mm and the cement dosage titration results decrease significantly, and the mixing uniformity of the mixture is significantly improved; (2) the 7-day and 28-day unconfined compressive strengths of the mixtures are significantly improved while the strength standard deviations and coefficients of variation are reduced, with an obvious trend along with the extension of the curing time (within 28 days).

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Sebastian A, Jomit T. Mathew, Tojo Jose, George K V, and Antony V T. "Efficacy of Embelia tsjeriam-cottam (Roem. & Schult.) A. DC. in Calcium Chelation." International Journal of Research in Pharmaceutical Sciences 11, no. 3 (2020): 4598–602. http://dx.doi.org/10.26452/ijrps.v11i3.2742.

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Embelia tsjeriam-cottam A. DC., commonly known as Vaividang, belongs to the family Myrsinaceae. Embelin, obtained from E. tsjeriam-cottam (an alternative source to Embelia ribes), is having diverse biological activities and used as anti-inflammatory, anti-diabetic, antimicrobial, anticancer and antioxidant. The present study aims to investigate the efficacy of Embelia tsjeriam-cottam fruit extract to chelate calcium under in vitro conditions. The findings of the study point to the possibility of using Embelia tsjeriam-cottam fruit extract as herbal calcium chelator, a substitute to EDTA. Embelia tsjeriam-cottam fresh fruits were shade dried and powdered. Fruit extract was prepared in 90 per cent ethanol by cold extraction method. Approximately 0.5 M CaCl2.2H2O solution was combined with 50 ml of an appropriately diluted fruit extract. The mixture was kept undisturbed for 24 hours under ambient temperature. After the incubation period, the mixture was centrifuged at ambient temperature at 4500 rpm for 15 min. The centrifugate was used for estimating leftover calcium ions. Calcium ions present in centrifugates was determined using complexometric titration with standardised EDTA solution as the titrant. Excess EDTA is challenging for the kidneys to eliminate and can even lead to kidney failure in extreme cases. It is evident that Embelia tsjeriam-cottam,has strong Ca2+ chelating properties, can be used as an alternative to EDTA in chelation therapy treatments.

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Monfared, Yousef Khazaei, Mohammad Mahmoudian, Gjylije Hoti, et al. "Hyper-Branched Cyclodextrin-Based Polymers as Anticoagulant Agents: In Vitro and In Vivo Studies." Bioengineering 9, no. 12 (2022): 765. http://dx.doi.org/10.3390/bioengineering9120765.

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This study tested the anticoagulant effect of cyclodextrin (CD) hyper-branched-based polymers (HBCD-Pols). These polymers were synthesized and tested for their coagulant characteristics in vitro and in vivo. Due to their polymeric structure and anionic nature, the polymers can chelate Ca2+, reducing the free quantity in blood. HBCD-Pol increased the blood clotting time, PT, and aPTT 3.5 times over the control, showing a better effect than even ethylenediaminetetraacetic acid (EDTA), as occured with recalcification time as well. A titration of HBCD-Pol and EDTA showed exciting differences in the ability to complex Ca2+ between both materials. Before executing in vivo studies, a hemocompatibility study was carried out with less than 5% red blood cell hemolysis. The fibrinogen consumption and bleeding time were analyzed in vivo. The fibrinogen was considerably decreased in the presence of HBCD-Pol in a higher grade than EDTA, while the bleeding time was longer with HBCD-Pols. The results demonstrate that the anticoagulant effect of this HBCD-Pol opens novel therapy possibilities due to the possible transport of drugs in this carrier. This would give combinatorial effects and a potential novel anticoagulant therapy with HBCD-Pol per se.

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Shyichuk, Alexander, Maria Kowalska, Iryna Shyychuk, Jan Lamkiewicz, and Dorota Ziółkowska. "Determination of Calcium in Meat Products by Automatic Titration with 1,2-Diaminocyclohexane-N,N,N’,N’-tetraacetic Acid." Molecules 28, no. 18 (2023): 6592. http://dx.doi.org/10.3390/molecules28186592.

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Mechanically separated meat (MSM) is a by-product of the poultry industry that requires routine quality assessment. Calcium content is an indirect indicator of bone debris in MSM but is difficult to determine by EDTA titration due to the poor solubility of calcium phosphate. Therefore, 1,2-diaminocyclohexane-N,N,N’,N’-tetraacetic acid was used instead, which has two orders of magnitude higher affinity for calcium ions. In addition, the auxiliary complexing agents triethanolamine and Arsenazo III, an indicator that is sensitive to low calcium concentrations, were used. Automatic titration endpoint detection was performed using an immersion probe at 660 nm. It has been shown that the color change in Arsenazo III can also be read with an RGB camera. The CDTA titration procedure has been tested on commercial Bologna-type sausages and the results were in line with AAS and ICP reference data. The content of calcium in sausages turned out to be very diverse and weakly correlated with the content of MSM. The tested MSM samples had a wide range of calcium content: from 62 to 2833 ppm. Calcium-rich poultry by-products include fat and skin (115 to 412 ppm), articular cartilage (1069 to 1704 ppm), and tendons (532 to 34,539 ppm). The CDTA titration procedure is fully suitable for small meat processing plants due to its simplicity of use and low cost.

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Lee, Phillip, Lorraine Green, Bartosz Marzec, Fiona Meldrum, Galdo Francesco Del, and Begonya Alcacer-Pitarch. "Dystrophic calcinosis: structural and morphological composition, and evaluation of ethylenediaminetetraacetic acid ('EDTA') for potential local treatment." Arthritis Research & Therapy 26, no. 1 (2024): 102. https://doi.org/10.1186/s13075-024-03324-7.

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Background: To perform a detailed morphological analysis of the inorganic portion of two different clinical presentations of calcium-based deposits retrieved from subjects with SSc and identify a chemical dissolution of these deposits suitable for clinical use.Methods: Chemical analysis using Fourier Transform IR spectroscopy ('FTIR'), Raman microscopy, Powder X-Ray Diffraction ('PXRD'), and Transmission Electron Microscopy ('TEM') was undertaken of two distinct types of calcinosis deposits: paste and stone. Calcinosis sample titration with ethylenediaminetetraacetic acid ('EDTA') assessed the concentration at which the EDTA dissolved the calcinosis deposits in vitro.Results: FTIR spectra of the samples displayed peaks characteristic of hydroxyapatite, where signals attributable to the phosphate and carbonate ions were all identified. Polymorph characterization using Raman spectra were identical to a hydroxyapatite reference while the PXRD and electron diffraction patterns conclusively identified the mineral present as hydroxyapatite. TEM analysis showed differences of morphology between the samples. Rounded particles from stone samples were up to a few micron in size, while needle-like crystals from paste samples reached up to 0.5 µm in length.Calcium phosphate deposits were effectively dissolved with 3% aqueous solutions of EDTA, in vitro. Complete dissolution of both types of deposit was achieved in approximately 30 min using a molar ratio of EDTA/HAp of ≈ 300.Conclusions: Stone and paste calcium-based deposits both comprise hydroxyapatite, but the constituent crystals vary in size and morphology. Hydroxyapatite is the only crystalline polymorph present in the SSc-related calcinosis deposits. Hydroxyapatite can be dissolved in vitro using a dosage of EDTA considered safe for clinical application. Further research is required to establish the optimal medium to develop the medical product, determine the protocol for clinical application, and to assess the effectiveness of EDTA for local treatment of dystrophic calcinosis.

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Nour El-Dien, F. A. "Some Observations on the Spectrophotometry Determination of Di-And Trihydric Phenols by Edta Titration." Spectroscopy Letters 33, no. 3 (2000): 347–57. http://dx.doi.org/10.1080/00387010009350081.

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Journal articles on the topic 'EDTA titration'

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