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1

Felicioli, Antonio, Mario Forzan, Simona Sagona та ін. "Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)". Veterinary Sciences 7, № 2 (2020): 52. http://dx.doi.org/10.3390/vetsci7020052.

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Honeybee pathogens have an important role in honeybee colony mortality and colony losses; most of them are widely spread and necessitate worldwide solutions to contrast honeybee’s decline. Possible accepted solutions to cope with the spread of honeybee’s pathogens are focused on the study of experimental protocols to enhance the insect’s immune defenses. Honeybee’s artificial diet capable to stimulate the immune system is a promising field of investigation as ascertained by the introduction of 1,3-1,6 β-glucans as a dietary supplement. In this work, by collecting faecal samples of honeybees ex
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Jung, Afonso H., Clériosn R. Perini, Ivair Valmorbida, et al. "Foraging, spatial distribution and the effect of honeybees on soybean yield." DECEMBER 2020, no. 14(12):2020 (December 10, 2020): 1983–90. http://dx.doi.org/10.21475/ajcs.20.14.12.2855.

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The soybean is the most cultivated grain crop in Brazil and there are many efforts to protect visitor pollinators, especially honeybees. The understanding of honeybee behavior on soybean fields is important to growers to apply integrated pest management strategies to avoid harm the pollinators. The European bee (Apis mellifera Linnaeus, 1758 (Hymenoptera: Apidae)) is a social bee, with European origin, whose the worker length is 12 mm to 13 mm with darker chest hairs. In this sense, foraging hours of Apis on soybean, its spatial distribution, the effect of pollination on soybean yield and the
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Chiari, Wainer César, Vagner de Alencar Arnaut de Toledo, Maria Claudia Colla Ruvolo-Takasusuki, et al. "Pollination of soybean (Glycine max L. Merril) by honeybees (Apis mellifera L.)." Brazilian Archives of Biology and Technology 48, no. 1 (2005): 31–36. http://dx.doi.org/10.1590/s1516-89132005000100005.

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This experiment was carried out to evaluate the effect of the honeybee pollination in the production and quality of soybean seeds (Glycine max L. Merril). Seed production was higher (P=0.0001) in covered areas with honeybee colonies (50.64%) and uncovered areas (57.73%) than in covered areas without honeybee colonies. It could be concluded that honeybees were responsible for 95.5% of the pollination accomplished by insects. The pod number in covered treatment with honeybees was 61.38% higher (P=0.0002) than in the covered treatment without honeybees. The average weight of 100 seeds was larger
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Varis, Anna-Liisa, and Raija Brax. "Effect of bee pollination on yield and yield components of field bean (Vicia faba L.)." Agricultural and Food Science 62, no. 1 (1990): 45–49. http://dx.doi.org/10.23986/afsci.72923.

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The effect of bees on the yield of field bean (Vicia faba L.) was studied in cage experiments in southern Finland during two successive years. Comparisons were made between field cages with and without honeybees and open-pollinated uncaged controls. Effect of shading was also considered. The yield in open plots, which were freely visited by pollinators, was considerably greater than in plots from which the insects were excluded. Freely visited plants and plants caged with bees produced about twice as many seeds as plants caged without bees, and the number of seeds per pod was also higher. The
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Gerber, B., and J. Ullrich. "No evidence for olfactory blocking in honeybee classical conditioning." Journal of Experimental Biology 202, no. 13 (1999): 1839–54. http://dx.doi.org/10.1242/jeb.202.13.1839.

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We use binary odorant compounds to investigate ‘blocking’ in honeybees which learn to associate an odorant (A-D) with a sucrose reward as the reinforcer (+). ‘Blocking’ means that learning about a stimulus B is reduced when trained in compound with a stimulus A that has previously been trained alone. Thus, reinforcement of B in these circumstances is not sufficient to induce learning. Such blocking is a frequently observed phenomenon in vertebrate learning and has also recently been reported in honeybee olfactory learning. To explain blocking, current models of conditioning include cognition-l
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Lindström, Sandra A. M., Lina Herbertsson, Maj Rundlöf, Riccardo Bommarco, and Henrik G. Smith. "Experimental evidence that honeybees depress wild insect densities in a flowering crop." Proceedings of the Royal Society B: Biological Sciences 283, no. 1843 (2016): 20161641. http://dx.doi.org/10.1098/rspb.2016.1641.

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While addition of managed honeybees ( Apis mellifera ) improves pollination of many entomophilous crops, it is unknown if it simultaneously suppresses the densities of wild insects through competition. To investigate this, we added 624 honeybee hives to 23 fields of oilseed rape ( Brassica napus L.) over 2 years and made sure that the areas around 21 other fields were free from honeybee hives. We demonstrate that honeybee addition depresses the densities of wild insects (bumblebees, solitary bees, hoverflies, marchflies, other flies, and other flying and flower-visiting insects) even in a mass
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STRAUSS, URSULA, VINCENT DIETEMANN, HANNELIE HUMAN, ROBIN M. CREWE, and CHRISTIAN W. W. PIRK. "Resistance rather than tolerance explains survival of savannah honeybees (Apis mellifera scutellata) to infestation by the parasitic mite Varroa destructor." Parasitology 143, no. 3 (2015): 374–87. http://dx.doi.org/10.1017/s0031182015001754.

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SUMMARYVarroa destructor is considered the most damaging parasite affecting honeybees (Apis mellifera L.). However, some honeybee populations such as the savannah honeybee (Apis mellifera scutellata) can survive mite infestation without treatment. It is unclear if survival is due to resistance mechanisms decreasing parasite reproduction or to tolerance mechanisms decreasing the detrimental effects of mites on the host. This study investigates both aspects by quantifying the reproductive output of V. destructor and its physiological costs at the individual host level. Costs measured were not co
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Williams, Ingrid H., A. P. Martin, and R. P. White. "The effect Of insect pollination on plant development and seed production in winter oil-seed rape (Brassica napus L.)." Journal of Agricultural Science 109, no. 1 (1987): 135–39. http://dx.doi.org/10.1017/s0021859600081077.

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SummaryThe effect of insect pollination on plant development and seed yield of winter oil-seed rape, cultivar Jet Neuf, was investigated by comparing plots caged with a honeybee colony, plots caged to exclude insects and plots uncaged and 'open-pollinated' by naturally occurring insects. Plants in the bee-pollinated plots finished flowering earlier, showed more advanced pod growth, and were shorter than those in the plots without bees. Pods from the plots with honeybees contained more seed post-flowering than those from plots without honeybees but the proportion of them that grew into mature s
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Hernández López, Javier, Wolfgang Schuehly, Karl Crailsheim, and Ulrike Riessberger-Gallé. "Trans-generational immune priming in honeybees." Proceedings of the Royal Society B: Biological Sciences 281, no. 1785 (2014): 20140454. http://dx.doi.org/10.1098/rspb.2014.0454.

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Maternal immune experience acquired during pathogen exposure and passed on to progeny to enhance resistance to infection is called trans-generational immune priming (TgIP). In eusocial insects like honeybees, TgIP would result in a significant improvement of health at individual and colony level. Demonstrated in invertebrates other than honeybees, TgIP has not yet been fully elucidated in terms of intensity and molecular mechanisms underlying this response. Here, we immune-stimulated honeybee queens with Paenibacillus larvae ( Pl ), a spore-forming bacterium causing American Foulbrood, the mos
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Matsumoto, Takashi. "Short- and long-term effects of neonicotinoid application in rice fields, on the mortality and colony collapse of honeybees (Apis mellifera)." Journal of Apicultural Science 57, no. 2 (2013): 21–35. http://dx.doi.org/10.2478/jas-2013-0014.

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Abstract Declines in honeybee (Apis mellifera ) colonies have elicited great concern worldwide. Recently, many Japanese beekeepers have implied that midsummer use of a new insecticide, neonicotinoid, in rice fields, is causing widespread mortality of neighboring honeybees and frequently resulting in colony collapse. Since few field experiments have directly tested the effects of neonicotinoids, I addressed four research questions in the field. The questions are: 1) Does clothianidin application in rice fields cause the collapse of neighboring honeybee colonies? 2) Is colony collapse related to
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Boot, Willem J., David J. A. Sisselaar, Johan N. M. Calis, and Joop Beetsma. "Factors affecting invasion of Varroa jacobsoni (Acari: Varroidae) into honeybee, Apis mellifera (Hymenoptera: Apidae), brood cells." Bulletin of Entomological Research 84, no. 1 (1994): 3–10. http://dx.doi.org/10.1017/s0007485300032168.

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AbstractTo study the rate of invasion into brood cells, a group of varroa mites, Varroa jacobsoni Oudemans, was introduced into honeybee colonies, Apis mellifera Linnaeus. For each experiment one or two otherwise comparable colonies was treated, and the effect on the rate of invasion was assessed. The results showed that the rate of invasion increases with the number of available brood cells, and decreases with the size of the honeybee population. This is expected when the rate of invasion is limited because the bees have to carry the mites close to a brood cell for invasion to occur, and only
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Du, Manuel, Richard Bernstein, Andreas Hoppe, and Kaspar Bienefeld. "Short-term effects of controlled mating and selection on the genetic variance of honeybee populations." Heredity 126, no. 5 (2021): 733–47. http://dx.doi.org/10.1038/s41437-021-00411-2.

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AbstractDirectional selection in a population yields reduced genetic variance due to the Bulmer effect. While this effect has been thoroughly investigated in mammals, it is poorly studied in social insects with biological peculiarities such as haplo-diploidy or the collective expression of traits. In addition to the natural adaptation to climate change, parasites, and pesticides, honeybees increasingly experience artificial selection pressure through modern breeding programs. Besides selection, many honeybee breeding schemes introduce controlled mating. We investigated which individual effects
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LIU, F. X., C. P. BU, T. TANG, et al. "Effects of Chinese honeybee foraging on oilseed rape gene flow and honey ingredients." Journal of Agricultural Science 155, no. 10 (2017): 1623–32. http://dx.doi.org/10.1017/s0021859617000776.

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SUMMARYHoneybee foraging can transfer exogenous genes from genetically modified (GM) oilseed rape (Brassica napusL.) to closely related plants, which not only induces potential ecological risks but also contaminates non-GM seeds or honey products with GM ingredients. These events may lead to international trade disputes. Chinese honeybees (Apis cerana ceranaFabricius) and a herbicide (glufosinate)-resistant GM strain ofB. napus(Z7B10) were studied to examine the effects of honeybee short-range foraging on oilseed rape gene flow and honey ingredients. Results showed variable frequencies of gene
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Pelz, C., B. Gerber, and R. Menzel. "Odorant intensity as a determinant for olfactory conditioning in honeybees: roles in discrimination, overshadowing and memory consolidation." Journal of Experimental Biology 200, no. 4 (1997): 837–47. http://dx.doi.org/10.1242/jeb.200.4.837.

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Stimulus intensity is an important determinant for perception, learning and behaviour. We studied the effects of odorant concentration on classical conditioning involving odorants and odorant-mechanosensory compounds using the proboscis-extension reflex in the honeybee. Our results show that high concentrations of odorant (a) support better discrimination in a feature-positive task using rewarded odorant-mechanosensory compounds versus unrewarded mechanosensory stimuli, (b) have a stronger capacity to overshadow learning of a simultaneously trained mechanosensory stimulus, and (c) induce bette
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Williams, I. H., J. R. Simpkins, and A. P. Martin. "Effect of insect pollination on seed production in linseed (Linum usitatissimum)." Journal of Agricultural Science 117, no. 1 (1991): 75–79. http://dx.doi.org/10.1017/s0021859600078989.

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SUMMARYThe effect of bee visits to the flowers on flower longevity and capsule, seed and oil production in linseed (Linum usitatissimum L.) cv. Antares, was investigated in two very different growing seasons (1988 and 1989); plots caged to exclude insects, plots caged with a honeybee colony and plots uncaged and open-pollinated by honeybees and bumblebees were compared. The experiments were conducted at Rothamsted Experimental Station Farm in Hertfordshire.Although honeybee visits to the flowers resulted in earlier petal fall, they did not increase capsule or seed production. It is concluded t
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Renner, Susanne S., Marie Sophie Graf, Zoe Hentschel, Helen Krause, and Andreas Fleischmann. "High honeybee abundances reduce wild bee abundances on flowers in the city of Munich." Oecologia 195, no. 3 (2021): 825–31. http://dx.doi.org/10.1007/s00442-021-04862-6.

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AbstractThe increase in managed honeybees (Apis mellifera) in many European cities has unknown effects on the densities of wild bees through competition. To investigate this, we monitored honeybees and non-honeybees from 01 April to 31 July 2019 and 2020 at 29 species of plants representing diverse taxonomic and floral-functional types in a large urban garden in the city of Munich in which the same plant species were cultivated in both years. No bee hives were present in the focal garden, and all bee hives in the adjacent area were closely monitored by interviewing the relevant bee keepers in
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He, Chunling, Kaiyue Zhang, Dongbo Han, Shuaibing Wang, Xiaogai Hou, and Chaodong Zhu. "Foraging Behavior of Honeybees (Apis Mellifera L.) and Ground Bumblebees (Bombus Terrestris L.) and its Influence on Seed Yield and Oil Quality of Oil Tree Peony Cultivar ‘Fengdan’ (Paeonia Ostii T. Hong et J. X. Zhang)." Journal of Apicultural Science 64, no. 1 (2020): 131–42. http://dx.doi.org/10.2478/jas-2020-0014.

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AbstractOil peony (Paeonia spp.) is a new type of woody oil crop in China with a large cultivation area. Inadequate pollination is one of the main reasons for low seed yield. A pollination net room was built at an oil tree peony base, the numbers of honeybees (Apis mellifera L.) and ground bumblebees (Bombus terrestris L.) were artificially increased and the foraging behaviors and daily activities of the two bees on the plants were observed. Four different pollination methods (honeybee pollination, ground bumblebee pollination, natural field pollination and pollination without insects) were ap
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Henry, Mickaël, Nicolas Cerrutti, Pierrick Aupinel, et al. "Reconciling laboratory and field assessments of neonicotinoid toxicity to honeybees." Proceedings of the Royal Society B: Biological Sciences 282, no. 1819 (2015): 20152110. http://dx.doi.org/10.1098/rspb.2015.2110.

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European governments have banned the use of three common neonicotinoid pesticides due to insufficiently identified risks to bees. This policy decision is controversial given the absence of clear consistency between toxicity assessments of those substances in the laboratory and in the field. Although laboratory trials report deleterious effects in honeybees at trace levels, field surveys reveal no decrease in the performance of honeybee colonies in the vicinity of treated fields. Here we provide the missing link, showing that individual honeybees near thiamethoxam-treated fields do indeed disap
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Favre, Daniel, and Olle Johansson. "DOES ENHANCED ELECTROMAGNETIC RADIATION DISTURB HONEYBEES’ BEHAVIOUR? OBSERVATIONS DURING NEW YEAR’S EVE 2019." International Journal of Research -GRANTHAALAYAH 8, no. 11 (2020): 7–14. http://dx.doi.org/10.29121/granthaalayah.v8.i11.2020.2151.

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Insects, and especially honeybees, are under major threat everywhere around the globe. Current studies lack in the consideration of potential effects which may directly affect other organisms or ecosystems, because of the very limited attention which is usually received by the potential adverse ecological effects of radiofrequency electromagnetic fields. Here, it is hypothesized that planetary enhancement of electromagnetic radiation produces a disturbing pollution for honeybees. In order to test this hypothesis, a bi-directional wide frequency range microphone was placed during the New Year’s
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Minozzi, Giulietta, Barbara Lazzari, Maria Grazia De Iorio, et al. "Whole-Genome Sequence Analysis of Italian Honeybees (Apis mellifera)." Animals 11, no. 5 (2021): 1311. http://dx.doi.org/10.3390/ani11051311.

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At the end of the last glaciation, Apis mellifera was established in northern Europe. In Italy, Apis melliferaligustica adapted to the mild climate and to the rich floristic biodiversity. Today, with the spread of Varroa destructor and with the increasing use of pesticides in agriculture, the Ligustica subspecies is increasingly dependent on human action for its survival. In addition, the effects of globalization of bee keeping favored the spread in Italy of other honeybee stocks of A. mellifera, in particular the Buckfast bee. The purpose of this study was to characterize the Italian honeybee
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Pizzaia, William Cristian da Silva, Tamiris de Oliveira Diniz, Breno Gabriel da Silva, et al. "Toxicological and morphological analysis of Africanized Apis mellifera selected for tolerance to the neonicotinoid thiamethoxam." Research, Society and Development 10, no. 2 (2021): e14310212109. http://dx.doi.org/10.33448/rsd-v10i2.12109.

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Honeybees are the insect most used for pollination purposes due to its efficient characteristics for this function, which reflects in positive aspects for both nature and man. The expansion of agriculture and the development of agrochemicals to combat pests has had negative impacts on honeybee health, causing its disappearance around the world. This research aimed to evaluate the effects of honeybee exposure to the neonicotinoid insecticide thiamethoxam on its survival rate, as well as on morphological and histological changes in the midgut of adult workers from the F4 generation of Apis melli
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Tauber, James P., Vy Nguyen, Dawn Lopez, and Jay D. Evans. "Effects of a Resident Yeast from the Honeybee Gut on Immunity, Microbiota, and Nosema Disease." Insects 10, no. 9 (2019): 296. http://dx.doi.org/10.3390/insects10090296.

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The western honeybee (Apis mellifera) has a core bacterial microbiota that is well described and important for health. Honeybees also host a yeast community that is poorly understood with respect to host nutrition and immunity, and also the symbiotic bacterial microbiota. In this work, we present two studies focusing on the consequences of dysbiosis when honeybees were control-fed a yeast that was isolated from a honeybee midgut, Wickerhamomyces anomalus. Yeast augmentation for bees with developed microbiota appeared immunomodulatory (lowered immunity and hormone-related gene expression) and a
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Perdriau, Benjamin S., and Mary R. Myerscough. "Making good choices with variable information: a stochastic model for nest-site selection by honeybees." Biology Letters 3, no. 2 (2007): 140–43. http://dx.doi.org/10.1098/rsbl.2006.0599.

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A density-dependent Markov process model is constructed for information transfer among scouts during nest-site selection by honeybees ( Apis mellifera ). The effects of site quality, competition between sites and delays in site discovery are investigated. The model predicts that bees choose the better of two sites more reliably when both sites are of low quality than when both sites are of high quality and that delay in finding a second site has most effect on the final choice when both sites are of high quality. The model suggests that stochastic effects in honeybee nest-site selection confer
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Tauber, James P., Cansu Ö. Tozkar, Ryan S. Schwarz, et al. "Colony-Level Effects of Amygdalin on Honeybees and Their Microbes." Insects 11, no. 11 (2020): 783. http://dx.doi.org/10.3390/insects11110783.

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Amygdalin, a cyanogenic glycoside, is found in the nectar and pollen of almond trees, as well as in a variety of other crops, such as cherries, nectarines, apples and others. It is inevitable that western honeybees (Apis mellifera) consistently consume amygdalin during almond pollination season because almond crops are almost exclusively pollinated by honeybees. This study tests the effects of a field-relevant concentration of amygdalin on honeybee microbes and the activities of key honeybee genes. We executed a two-month field trial providing sucrose solutions with or without amygdalin ad lib
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Mookhploy, Wannapha, Sasiprapa Krongdang, and Panuwan Chantawannakul. "Effects of Deformed Wing Virus Infection on Expressions of Immune- and Apoptosis-Related Genes in Western Honeybees (Apis mellifera)." Insects 12, no. 1 (2021): 82. http://dx.doi.org/10.3390/insects12010082.

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Honeybees are globally threatened by several pathogens, especially deformed wing virus (DWV), as the presence of DWV in western honeybees is indicative of colony loss. The high mortality rate is further exacerbated by the lack of effective treatment, and therefore understanding the immune and apoptosis responses could pave an avenue for the treatment method. In this study, DWV was directly injected into the white-eyed pupae stage of western honeybees (Apis mellifera). The DWV loads and selected gene responses were monitored using the real-time PCR technique. The results showed that honeybee pu
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Oldroyd, Benjamin P., Michael H. Allsopp, Katherine M. Roth, Emily J. Remnant, Robert A. Drewell, and Madeleine Beekman. "A parent-of-origin effect on honeybee worker ovary size." Proceedings of the Royal Society B: Biological Sciences 281, no. 1775 (2014): 20132388. http://dx.doi.org/10.1098/rspb.2013.2388.

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Apis mellifera capensis is unique among honeybees in that unmated workers can produce pseudo-clonal female offspring via thelytokous parthenogenesis. Workers use this ability to compete among themselves and with their queen to be the mother of new queens. Males could therefore enhance their reproductive success by imprinting genes that enhance fertility in their daughter workers. This possibility sets the scene for intragenomic conflict between queens and drones over worker reproductive traits. Here, we show a strong parent-of-origin effect for ovary size (number of ovarioles) in reciprocal cr
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Abramson, Charles I., and M. E. Bitterman. "The US-preexposure effect in honeybees." Animal Learning & Behavior 14, no. 4 (1986): 374–79. http://dx.doi.org/10.3758/bf03200081.

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Ptaszyńska, Aneta A., and Daniel Załuski. "Extracts from Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. Roots: A New Hope Against Honeybee Death Caused by Nosemosis." Molecules 25, no. 19 (2020): 4452. http://dx.doi.org/10.3390/molecules25194452.

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Pollinators, the cornerstones of our terrestrial ecosystem, have been at the very core of our anxiety. This is because we can nowadays observe a dangerous decline in the number of insects. With the numbers of pollinators dramatically declining worldwide, the scientific community has been growing more and more concerned about the future of insects as fundamental elements of most terrestrial ecosystems. Trying to address this issue, we looked for substances that might increase bee resistance. To this end, we checked the effects of plant-based adaptogens on honeybees in laboratory tests and durin
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Shawki M, A.-A., V. Táborský, F. Kamler, and J. Kazda. "Effect of two NeemAzalTM formulations on honeybees under semi-field conditions." Plant Protection Science 41, No. 2 (2010): 63–72. http://dx.doi.org/10.17221/2744-pps.

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The effects of NeemAzalTM formulations: NeemAzalTM T/S (1% azadirachtin) and NeemAzalTM granules (1% azadirachtin) on honeybees, <i>Apis mellifera</i> L., were studied under semi-field conditions. Three plots at 15 m<sup>2</sup> each were sown with spring rape seeds Brassica napus cultivar Likolly (Brassicaceae/Cruciferae). In the first plot NeemAzal granules were added with the seeds during sowing. The second plot was sprayed with NeemAzal T/S during full flowering; GreemaxTM was used as a wetting agent. The third one was sprayed with water only during full flowering a
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Glavan, Gordana. "Histochemical Staining of Acetylcholinesterase in Carnolian Honeybee (Apis mellifera carnica) Brain after Chronic Exposure to Organophosphate Diazinon." Journal of Apicultural Science 64, no. 1 (2020): 123–30. http://dx.doi.org/10.2478/jas-2020-0003.

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AbstractOrganophosphate insecticides are known to inhibit the activity of enzyme acetylcholinesterase. They affect olfactory learning and memory formation in honeybees. These insecticides cause mushroom body inactivation in honeybees, but their influence on other brain regions involved in olfactory perception and memory is unknown. The goal of this study was to study the effects of organophosphate insecticide diazinon on carnolian honeybee (Apis mellifera carnica) acetylcholinesterase activity in the olfactory brain regions of antennal lobe, mushroom body and lateral procerebrum (lateral horn)
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Pospiech, Matej, Simona Ljasovská, Dalibor Titěra, Vojtěch Kružík, Zdeňka Javůrková, and Bohuslava Tremlová. "Pollen diversity in honeys of the Czech Republic in the 2019 season." Potravinarstvo Slovak Journal of Food Sciences 14 (November 28, 2020): 1115–23. http://dx.doi.org/10.5219/1504.

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Honeybees are important pollinators. As a side product of pollination, honeybees produce honey, as a natural sweetener. The source of honey depends on the hive location. In specific conditions honeybees produce monofloral honey, but more common are polyfloral kinds of honey. In this study honey from the Czech Republic in the 2019 season was evaluated by melissopalynology analysis. The common botanical taxa in the Czech Republic were determined and season impact to pollen taxa was compared for dominant pollen taxa. The taxonomic distribution of pollen in Czech honey was stable during the year.
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Alberoni, Daniele, Loredana Baffoni, Chiara Braglia, Francesca Gaggìa, and Diana Di Gioia. "Honeybees Exposure to Natural Feed Additives: How Is the Gut Microbiota Affected?" Microorganisms 9, no. 5 (2021): 1009. http://dx.doi.org/10.3390/microorganisms9051009.

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The role of a balanced gut microbiota to maintain health and prevent diseases is largely established in humans and livestock. Conversely, in honeybees, studies on gut microbiota perturbations by external factors have started only recently. Natural methods alternative to chemical products to preserve honeybee health have been proposed, but their effect on the gut microbiota has not been examined in detail. This study aims to investigate the effect of the administration of a bacterial mixture of bifidobacteria and Lactobacillaceae and a commercial product HiveAliveTM on honeybee gut microbiota.
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Loope, Kevin J., James W. Baty, Philip J. Lester, and Erin E. Wilson Rankin. "Pathogen shifts in a honeybee predator following the arrival of the Varroa mite." Proceedings of the Royal Society B: Biological Sciences 286, no. 1894 (2019): 20182499. http://dx.doi.org/10.1098/rspb.2018.2499.

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Emerging infectious diseases (EIDs) are a global threat to honeybees, and spillover from managed bees threaten wider insect populations. Deformed wing virus (DWV), a widespread virus that has become emergent in conjunction with the spread of the mite Varroa destructor , is thought to be partly responsible for global colony losses. The arrival of Varroa in honeybee populations causes a dramatic loss of viral genotypic diversity, favouring a few virulent strains. Here, we investigate DWV spillover in an invasive Hawaiian population of the wasp, Vespula pensylvanica , a honeybee predator and hone
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Gupta, Deepali, Harsha Chauhan, Sheifali Gupta, and Rupesh Gupta. "Effect of Colony Collapse Disorder on Honeybees." Journal of Computational and Theoretical Nanoscience 16, no. 10 (2019): 4149–52. http://dx.doi.org/10.1166/jctn.2019.8494.

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Bees play a vital role in saving environment as they are the paramount agricultural pollinators and are the prior pollinators in the tropical ecosystem. Now a days, bees are in trouble as they are suffering from a mysterious condition known as colony collapse disorder in which honeybees leave their hives but fail to return back there because of the environmental activities done by the human. It caused a tremendous drop in the number of bees around the globe. The main reasons behind it are massive use of pesticides in agriculture, trading of bees by the humans and electromagnetic radiation emit
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35

Padma-Shree, I., S. S. J. Roseleen, and C. G. L. Justin. "Effect of probiotic supplement feed on the foraging activity of Indian honeybee (Apis cerana indica F.)." Journal of Environmental Biology 42, no. 4(SI) (2021): 1093–97. http://dx.doi.org/10.22438/jeb/42/4(si)/mrn-1519b.

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Aim: To investigate the effect of probiotic supplement feed on the foraging behaviour Indian honeybee colonies to resolve the excessive usage of antibiotics. Methodology: The effect of sugar syrup feed (Untreated hives) and sugar syrup + probiotic supplement feed @ 10 ml 100 ml-1 syrup (Treated hives) on Indian honeybees was tested. The foraging activity and foraging rate were recorded and subjected to paired t- test analysis. Results: The present observation showed a significant difference in the foraging rate between the colonies fed with sugar syrup + probiotic supplement (2.33 ± 0.11 min-1
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36

Denton, Jai A., Ivan Koludarov, Michele Thompson, Jarosław Bryk, and Mariana Velasque. "Honeybee Cognition as a Tool for Scientific Engagement." Insects 12, no. 9 (2021): 842. http://dx.doi.org/10.3390/insects12090842.

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Apis mellifera (honeybees) are a well-established model for the study of learning and cognition. A robust conditioning protocol, the olfactory conditioning of the proboscis extension response (PER), provides a powerful but straightforward method to examine the impact of varying stimuli on learning performance. Herein, we provide a protocol that leverages PER for classroom-based community or student engagement. Specifically, we detail how a class of high school students, as part of the Ryukyu Girls Outreach Program, examined the effects of caffeine and dopamine on learning performance in honeyb
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Choi, Seok Hwa, Seong Soo Kang, Chun Sik Bae, Seong Koo Cho, and Sok Cheon Pak. "Effect of Bee Venom Treatment in Sows with Oligogalactic Syndrome Postpartum." American Journal of Chinese Medicine 31, no. 01 (2003): 149–55. http://dx.doi.org/10.1142/s0192415x03000801.

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The objective of this study was to determine the clinico-therapeutic effect of worker honeybee venom in sows with oligogalactic syndrome postpartum. Comparison between bee venom- and drug-treated groups was our main concern in the present study. Sows after parturition were assigned to bee venom- and drug-treated groups, respectively. In the bee venom-treated group, 22 sows were bee-acupunctured once a day for 3 consecutive days. Honeybees (Apis mellifera L.) for bee acupuncture were about 15 days old after metamorphosis. Live bees were used to sting the acupoints known as Yang-ming (ST-18, 1.5
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Chen, Xiao, and Jinluan Fu. "The microRNA miR-14 Regulates Egg-Laying by Targeting EcR in Honeybees (Apis mellifera)." Insects 12, no. 4 (2021): 351. http://dx.doi.org/10.3390/insects12040351.

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Honeybees (Apis mellifera) are important pollinators and are commonly used for honey production. The oviposition behavior in honeybees is complex and errors in oviposition could affect the development of the bee colony. Recent studies reported that RNA–RNA cross-talk played a critical role in several biological processes, including reproduction. Ecdysone receptor (EcR) and miR-14 were previously reported to play important roles in egg-laying. Moreover, EcR was predicted to be the target gene of miR-14 and may form miR-14-EcR cross-talk. In this study, knocking down and overexpression of miR-14
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39

Tlak Gajger, I., J. Ribaric, M. Matak, et al. "Zeolite clinoptilolite as a dietary supplement and remedy for honeybee (Apis mellifera L.) colonies." Veterinární Medicína 60, No. 12 (2017): 696–705. http://dx.doi.org/10.17221/8584-vetmed.

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Control of the nosema disease poses a major challenge, and therefore, treatment of this serious parasitic disease using natural preparations could be of great benefit. The aim of this study was to test the performance of zeolite clinoptilolite as a curative measure against honeybee colonies (Apis mellifera L.) naturally infected by Nosema ceranae. The histopathological structure, and the content and distribution of mucosubstances and histochemical activity of aminopeptidase and non-specific esterase in the midgut mucosa of honeybees originating from colonies fed sugar syrup supplemented with z
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Kasiotis, Manea-Karga, and Machera. "A Zwitterionic Hydrophilic Interaction Liquid Chromatographic Photo Diode Array Method as a Tool to Investigate Oxalic Acid in Bees: Comparison with Mass Spectrometric Methods." Separations 6, no. 4 (2019): 48. http://dx.doi.org/10.3390/separations6040048.

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Background: Oxalic acid constitutes beekeepers′ leading choice for the battle of the parasitic mite Varroa destructor considering its efficacy, low cost, and easy application. Nevertheless, its broad use and reported synergistic effects prompted us to explore analytical methodologies for its determination in honeybees, especially after death incidents. Methods: The extraction of oxalic acid from bees was conducted by applying a simplified water extraction protocol. Oxalic acid′s content in honeybees was investigated through a novel zwiterionic hydrophilic interaction liquid chromatographic cou
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Al-Kahtani, Saad N., El-Kazafy Taha, Khalid Ali Khan, et al. "Effect of harvest season on the nutritional value of bee pollen protein." PLOS ONE 15, no. 12 (2020): e0241393. http://dx.doi.org/10.1371/journal.pone.0241393.

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Bee pollen is a natural product that has valuable nutritional and medicinal characteristics and has recently garnered increasing attention in the food industry due to its nutritive value. Here, we harvested pollen loads from the Al-Ahsa oasis in eastern Saudi Arabia during spring, summer, autumn, and winter in 2018/2019 to compare the nutritional value of bee pollen protein with the amino acid requirements of honeybees and adult humans. Based on the nutritional value of bee pollen protein, the optimal season for harvesting bee pollen was determined. The composition of the bee pollen showed the
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42

Kekeçoğlu, Meral. "Morphometric Divergence of Anatolian Honeybees through Loss of Original Traits: A Dangerous Outcome of Turkish Apiculture." Sociobiology 65, no. 2 (2018): 232. http://dx.doi.org/10.13102/sociobiology.v65i2.1895.

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Five honeybee subspecies exist naturally in Anatolia. Unfortunately, owing largely to migratory beekeeping and lack of control mechanisms against requeening, the native honey bee subspecies located in Anatolia are facing extinction. Beekeeping activities, especially migratory beekeeping jeopardizes the presence of the naturally evolved indigenous subspecies of Anatolia. The present study examined morphological deformation in three Apis mellifera (L.) subspecies (A. m. caucasica, A. m. carnica, A. m. syriaca) and two ecotypes of A. m. anatoliaca (Muğla and Yığılca) that have been kept all toget
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43

Shinoda, Akira, and M. E. Bitterman. "Analysis of the overlearning-extinction effect in honeybees." Animal Learning & Behavior 15, no. 1 (1987): 93–96. http://dx.doi.org/10.3758/bf03204909.

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44

Wei, Hao, Xu Jiang He, Chun Hua Liao, et al. "A Maternal Effect on Queen Production in Honeybees." Current Biology 29, no. 13 (2019): 2208–13. http://dx.doi.org/10.1016/j.cub.2019.05.059.

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45

Peng, Tianfei, Maximilian Schroeder, and Christoph Grüter. "Octopamine increases individual and collective foraging in a neotropical stingless bee." Biology Letters 16, no. 6 (2020): 20200238. http://dx.doi.org/10.1098/rsbl.2020.0238.

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The biogenic amine octopamine (OA) is a key modulator of individual and social behaviours in honeybees, but its role in the other group of highly eusocial bees, the stingless bees, remains largely unknown. In honeybees, OA mediates reward perception and affects a wide range of reward-seeking behaviours. Thus, we tested the hypothesis that OA increases individual foraging effort and collective food source exploitation in the neotropical stingless bee Plebeia droryana . OA treatment caused a significant increase in the number of bees at artificial sucrose feeders and a 1.73-times higher individu
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He, Xu Jiang, Hao Wei, Wu Jun Jiang, Yi Bo Liu, Xiao Bo Wu, and Zhijiang Zeng. "Honeybee (Apis mellifera) Maternal Effect Causes Alternation of DNA Methylation Regulating Queen Development." Sociobiology 68, no. 1 (2021): 5935. http://dx.doi.org/10.13102/sociobiology.v68i1.5935.

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Queen-worker caste dimorphism is a typical trait for honeybees (Apis mellifera). We previously showed a maternal effect on caste differentiation and queen development, where queens emerged from queen-cell eggs (QE) had higher quality than queens developed from worker cell eggs (WE). In this study, newly-emerged queens were reared from QE, WE, and 2-day worker larvae (2L). The thorax size and DNA methylation levels of queens were measured. We found that queens emerging from QE had significantly larger thorax length and width than WE and 2L. Epigenetic analysis showed that QE/2L comparison had t
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47

Blanche, K. R., M. Hughes, J. A. Ludwig, and S. A. Cunningham. "Do flower-tripping bees enhance yields in peanut varieties grown in north Queensland?" Australian Journal of Experimental Agriculture 46, no. 11 (2006): 1529. http://dx.doi.org/10.1071/ea05190.

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It has been demonstrated that tripping of peanut flowers by large bees enhances pollination and improves peanut yields of some early commercial peanut varieties but this phenomenon has not been evaluated for recently developed peanut varieties. Our study aimed to establish whether bees provide this service for peanut varieties currently grown on the Atherton Tableland, north Queensland. To measure the impact of native and introduced bees occurring without assistance in crops, we set up 3 cage treatments (meshed to exclude large bees; partly meshed to allow bee access but take cage effects into
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48

Tomé, Hudson V. V., Gabryele S. Ramos, Micaele F. Araújo, et al. "Agrochemical synergism imposes higher risk to Neotropical bees than to honeybees." Royal Society Open Science 4, no. 1 (2017): 160866. http://dx.doi.org/10.1098/rsos.160866.

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Bees are key pollinators whose population numbers are declining, in part, owing to the effects of different stressors such as insecticides and fungicides. We have analysed the susceptibility of the Africanized honeybee, Apis mellifera , and the stingless bee, Partamona helleri, to commercial formulations of the insecticides deltamethrin and imidacloprid. The toxicity of fungicides based on thiophanate-methyl and chlorothalonil were investigated individually and in combination, and with the insecticides. Results showed that stingless bees were more susceptible to insecticides than honeybees. Th
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Bartling, Vilcinskas, and Lee. "Sub-Lethal Doses of Clothianidin Inhibit the Conditioning and Biosensory Abilities of the Western Honeybee Apis mellifera." Insects 10, no. 10 (2019): 340. http://dx.doi.org/10.3390/insects10100340.

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Insects play an important role in the stability of ecosystems by fulfilling key functions such as pollination and nutrient cycling, as well as acting as prey for amphibians, reptiles, birds and mammals. The global decline of insects is therefore a cause for concern, and the role of chemical pesticides must be examined carefully. The lethal effects of insecticides are well understood, but sub-lethal concentrations have not been studied in sufficient detail. We therefore used the western honeybee Apis mellifera as a model to test the effect of the neonicotinoid insecticide clothianidin on the mo
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Lu, Yun-Heng, Carol-P. Wu, Cheng-Kang Tang, et al. "Identification of Immune Regulatory Genes in Apis mellifera through Caffeine Treatment." Insects 11, no. 8 (2020): 516. http://dx.doi.org/10.3390/insects11080516.

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Plants and pollinators are mutually beneficial: plants provide nectar as a food source and in return their pollen is disseminated by pollinators such as honeybees. Some plants secrete chemicals to deter herbivores as a protective measure, among which is caffeine, a naturally occurring, bitter tasting, and pharmacologically active secondary compound. It can be found in low concentrations in the nectars of some plants and as such, when pollinators consume nectar, they also take in small amounts of caffeine. Whilst caffeine has been indicated as an antioxidant in both mammals and insects, the eff
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