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1
Fung, Shing-Tack, Cyrus K. Ho, Siu-Wai Choi, Wai-Yuen Chung, and Iris F. F. Benzie. "Comparison of catechin profiles in human plasma and urine after single dosing and regular intake of green tea (Camellia sinensis)." British Journal of Nutrition 109, no. 12 (2012): 2199–207. http://dx.doi.org/10.1017/s0007114512004370.
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Green tea (Camellia sinensis) catechin profiles in plasma and urine following single dosing and regular ingestion of green tea are not clear. We performed a placebo-controlled intervention study with sixteen healthy volunteers to determine changes in total and free catechins after a single dose and following 1 week of twice-daily green tea. Blood and urine samples were collected before (fasting) and after (60 and 120 min for blood; 90 and 180 min for urine) drinking 200 ml of 1·5 % (w/v) green tea or water (n 8 each), and fasting samples were again collected after 7 d of 150 ml of 1 % (w/v) supplemental green tea or water twice daily. After a 4-week washout, subjects were crossed onto the other treatment and procedures repeated. Plasma results at 1 h post-ingestion showed elevated (P< 0·05) mean epigallocatechin gallate (EGCG; 310 (sd 117) nmol/l; all in free form), epigallocatechin (EGC; 192 (sd 67) nmol/l; 30 % free) and epicatechin gallate (ECG; 134 (sd 51) nmol/l; 75 % free). Fasting plasma after 7 d of regular intake showed increased (P< 0·05) EGCG (80 v. 15 nmol/l at baseline) and ECG (120 v. 40 nmol/l), with ≥ 90 % of both in their conjugated forms. Total EGC was < 10 nmol/l. Post-ingestion conjugation and renal loss of EGC and epicatechin were rapid and high, but were negligible for EGCG and ECG. In the green tea consumed, the content was EGCG >EGC >ECG, and the acute plasma response mirrored this. However, after chronic consumption there was almost no EGC found in fasting plasma, some EGCG was present, but a rather high level of ECG was maintained.
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Piechocka, Justyna, Anna Gramza-Michałowska, and Krystyna Szymandera-Buszka. "The Changes in Antioxidant Activity of Selected Flavonoids and Caffeine Depending on the Dosage and Form of Thiamine." Molecules 26, no. 15 (2021): 4702. http://dx.doi.org/10.3390/molecules26154702.
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Phenolic compounds and thiamine may serve as therapies against oxidative stress-related neurodegenerative diseases. However, it is important to note that these components show high instability under changing conditions. The study’s aim was to determine the impact of the thiamine concentration (hydrochloride—TH and pyrophosphate—TP; in the range 0.02 to 20 mg/100 g on the indices of the chelating properties and reducing power, and free radicals scavenging indices of EGCG, EGC, ECG and caffeine added from 0.04 to 6.0 mg/100 g. Our research confirmed that higher concentrations of TH and TP can exhibit significant activity against the test antioxidant indices of all components. When above 5.0 mg/100 g of thiamine was used, the radical scavenging abilities of the compound decreased in the following order: EGCG > ECG > EGC > caffeine. The highest correlation was found for the concentration of thiamine pyrophosphate to 20.0 mg/100 g and EGCG. Knowledge of the impact of factors associated with the concentration of both EGCG, EGC, ECG or caffeine and thiamine on their activity could carry weight in regulating the quality supplemented foods, especially of nutrition support for people of all ages were oral, enteral tube feeding and parenteral nutrition).
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Li, Xuan, Shiyu Liu, Jin Yan, et al. "The Characteristics, Prognosis, and Risk Factors of Lymph Node Metastasis in Early Gastric Cancer." Gastroenterology Research and Practice 2018 (2018): 1–7. http://dx.doi.org/10.1155/2018/6945743.
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Background. Lymph node metastasis (LNM) is the most important risk factor for endoscopic treatment in early gastric cancer (EGC) patients. We aimed to investigate the rate of LNM, the risk factors, and the prognosis of EGC patients with LMN. Methods. A total of 10,039 patients who underwent gastrectomy with lymphadenectomy were reviewed between January 2010 and December 2015 at Jiangsu Province Hospital in China. Among them, we identified 1004 (10%) EGCs. First, endoscopic and clinicopathological features related to LNM were analyzed, and then risk factors for LNM were identified using univariate and multivariate analysis. Finally, the short- and long-term outcomes were compared between the groups. Results. LNM occurred in 123 (12.3%) EGCs. Most of EGCs were male (n=720, 71.7%) and mean age was 59.65 ± 11.09 years. The rate of H. pylori infection was 78.0% (783/1004). LNM was significantly associated with age, sex, location, lesion size, macroscopic type, depth of invasion, differentiation type, histological morphology, lymphovascular invasion (LVI), and TMN stage. By multivariate analysis, significant independent risk factors for LNM in EGC were identified as following: male sex (OR 2.365, P=0.035), age ≦ 40 (OR 0.055, P=0.012), depressed type (OR 2.721, P=0.013), submucosa invasion (OR 2.987, P=0.032), LVI (OR 5.186, P=0.003), tumor located in corpora (OR 8.904, P=0.047), and in angle (OR 12.998, P=0.024). 86.5% were successfully followed up for 3 years. The overall 1- and 3-year survival rates in LNM group were 100% and 91.1%, respectively, and those with no LNM were 100% and 100%, respectively. Conclusion. EGCs were investigated in 10.0% of gastric cancer, which LNM occurred in 12.3% of EGC. Independent risk factors of LNM included male sex, age (>40), the depth of invasion, LVI, and tumor located in corpora or angle. The 3-year overall survival rate was greater in EGC patients without LNM.
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Koch, Wojciech, Wirginia Kukula-Koch, and Łukasz Komsta. "Black Tea Samples Origin Discrimination Using Analytical Investigations of Secondary Metabolites, Antiradical Scavenging Activity and Chemometric Approach." Molecules 23, no. 3 (2018): 513. http://dx.doi.org/10.3390/molecules23030513.
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A comprehensive study on the composition and antioxidant properties of black tea samples with a chemometric approach was performed via LC-ESI-Q-TOF-MS, DPPH radical scavenging assay, and Folin–Ciocalteu assay (TPC). Marked differences between the teas from seven different countries (China, India, Iran, Japan, Kenya, Nepal, Sri Lanka) were shown. The Indian samples demonstrated the highest total catechin content (184.8 mg/100 mL), the largest TPC and DPPH scavenging potential (58.2 mg/100 mL and 84.5%, respectively). The applied principal component analysis (PCA) and ANOVA revealed several correlations between the level of catechins in tea infusions. EC (epicatechin), ECG (epicatechin gallate), EGC (epigallocatechin), and EGCG (epigallocatechin-3-gallate) content was not correlated with DPPH, gallic acid, and TPC; however, a strong correlation of EC and ECG between themselves and a negative correlation of these two catechins with EGCG and EGC was noted. Interestingly, simple catechins were not found to be responsible for antioxidant properties of the black teas. The samples collected in the higher altitudes were similar.
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Li, Na, Dongdong Xin, Hongbo Li, et al. "Epigallocatechin-3-Gallate Protects Neuro-2a Cells From Sodium Fluoride-Induced Oxidative Damage In Vitro." Natural Product Communications 15, no. 3 (2020): 1934578X2091147. http://dx.doi.org/10.1177/1934578x20911476.
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Fluoride is an essential trace element, but its beneficial range is narrow, and excess fluoride may have negative health effects. The objective of this study was to investigate the potential cytoprotective effects of epigallocatechin-3-gallate (EGCG) in cultured neuro-2a neuroblastoma cells exposed to sodium fluoride (NaF)-induced oxidative stress. Isolated Neuro-2a cells were exposed to increasing concentrations of NaF (0, 1, 2, 4, 6, and 8 mM) for 24 hours to induce oxidative stress. Moreover, to determine the concentration of EGCG necessary for protective effects, we exposed isolated Neuro-2a cells to increasing concentrations of EGCG (0, 0.5, 1, 5, 10, 20, 40, 60, 80, and 100 μg/mL) for 24 and 48 hours. Pretreatment with EGCG at various doses (0, 0.5, 1, 5, 10, 20, and 40 μg/mL) was evaluated in Neuro-2a cells for 24 hours, followed by an NaF (4 mM per culture well) challenge for 24 hours. As shown in this study, EGCG can protect Neuro-2a cells from NaF-induced apoptosis. This effect may be due to the reactive oxygen species scavenging activity of EGCC.
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Sim, Eun Hui, Byung-Wook Kim, Ji Hee Kim, and Jin-Jo Kim. "Long-term outcome of endoscopic submucosal dissection for early gastric cancer compared to surgical resection." Journal of Clinical Oncology 32, no. 3_suppl (2014): 120. http://dx.doi.org/10.1200/jco.2014.32.3_suppl.120.
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120 Background: Endoscopic submucosal dissection (ESD) is now accepted as an alternative to surgery for the treatment of early gastric cancer (EGC). However, long-term clinical outcome of ESD for EGC compared to surgical resection has not been evaluated. The aim of this study is to evaluate the clinical outcome of ESD for EGC compared to surgical resection. Methods: A retrospective analysis was performed in 152 patients who underwent ESD or surgical resection for EGC according to Gotoda’s extended criteria from 2006 and 2008 in Incheon St. Mary’s Hospital and Seoul St. Mary’s Hospital, The Catholic University of Korea. Overall survival and recurrence rates were compared between the two groups. Results: A total of 56 patients underwent surgical gastrectomy and 96 patients underwent ESD. The medial follow-up was 76 months in surgical resection group and 71 months in ESD group. Metachronous recurrences including dysplasia were found in 9 patients in ESD group and none in surgical resection group (P=0.001). There was no significant difference between the groups in overall survival. Conclusions: Gotoda’s extended criteria for ESD might be acceptable for the treatment of EGC considering the oval survival. However, meticulous surveillance program should be established because metachronous recurrence is more common after ESD.
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Ze Xu, Jin, Sai Ying Venus Yeung, Qi Chang, Yu Huang, and Zhen-Yu Chen. "Comparison of antioxidant activity and bioavailability of tea epicatechins with their epimers." British Journal of Nutrition 91, no. 6 (2004): 873–81. http://dx.doi.org/10.1079/bjn20041132.
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Canned and bottled tea drinks contain not only green tea epicatechins (GTE), namely (−)-epigallocatechin gallate (EGCG), (−)-epicatechin gallate (ECG), (−)-epigallocatechin (EGC) and (−)-epicatechin (EC), but also four GTE epimers, namely (−)-gallocatechin gallate (GCG), (−)-catechin gallate (CG), (−)-gallocatechin (GC) and (−)-catechin (C). In the present study we examined the antioxidant activity and bioavailability of these epimers compared with their corresponding precursors. The epimerisation reaction was induced by autoclaving GTE extract derived from longjing green tea at 120°C for 20 min. Isolation and purification of each GTE and epimer were accomplished by various column chromatographic and semi-preparative HPLC techniques. The antioxidant activity of each epimer with its corresponding GTE precursor was conducted in the three in vitro systems, namely human LDL oxidation, ferric reducing–antioxidant power (FRAP), and anti-2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assays. The results of all three assays demonstrated that CG had similar antioxidant activity with its precursor ECG, while GC was less potent as an antioxidant than its precursor EGC. Regarding EGCG and GCG, the antioxidant potency was similar for both LDL oxidation and DPPH free radical assays, but GCG was statistically less effective than EGCG in the FRAP assay. For EC and C, the latter had less anti-free radical activity in the DPPH assay, but in LDL oxidation and FRAP assays the antioxidant activity was similar. Oral and intravenous dosing of GTE–epimer mixture led to increase in total plasma antioxidant capacity in rats. In general, both epicatechins and epimers had low bioavailability (0·08–0·31) and most of the observed differences between epicatechins and their corresponding epimers were small, even if they were statistically significant in some cases. It was concluded that the epimerisation reaction occurring in manufacturing canned and bottled tea drinks would not significantly affect antioxidant activity and bioavailability of total tea polyphenols.
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Matsumoto, Kohei, Hiroya Ueyama, Takashi Yao, et al. "Diagnostic limitations of magnifying endoscopy with narrow-band imaging in early gastric cancer." Endoscopy International Open 08, no. 10 (2020): E1233—E1242. http://dx.doi.org/10.1055/a-1220-6389.
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Abstract Background and study aims Magnifying endoscopy with narrow band imaging (M-NBI) has made a huge contribution to endoscopic diagnosis of early gastric cancer (EGC). However, we sometimes encountered false-negative cases with M-NBI diagnosis (i. e., M-NBI diagnostic limitation lesion: M-NBI-DLL). However, clinicopathological features of M-NBI-DLLs have not been well elucidated. We aimed to clarify the clinicopathological features and histological reasons of M-NBI-DLLs. Patients and methods In this single-center retrospective study, M-NBI-DLLs were extracted from 456 EGCs resected endoscopically at our hospital. We defined histological types of M-NBI-DLLs and analyzed clinicopathologically to clarify histological reasons of M-NBI-DLLs. Results Of 456 EGCs, 48 lesions (10.5 %) of M-NBI-DLLs were enrolled. M-NBI-DLLs was classified into four histological types as follows: gastric adenocarcinoma of fundic-gland type (GA-FG, n = 25), gastric adenocarcinoma of fundic-gland mucosal type (GA-FGM, n = 1), differentiated adenocarcinoma (n = 14), and undifferentiated adenocarcinoma (n = 8). Thirty-nine lesions of M-NBI-DLLs were H. pylori-negative gastric cancers (39/47, 82.9 %). Histological reasons for M-NBI-DLLs were as follows: 1) completely covered with non-neoplastic mucosa (25/25 GA-FG, 8/8 undifferentiated adenocarcinoma); 2) well-differentiated adenocarcinoma with low-grade atypia (1/1 GA-FGM, 14/14 differentiated adenocarcinoma); 3) similarity of surface structure (10/14 differentiated adenocarcinoma); and 4) partially covered and/or mixed with a non-neoplastic mucosa (1/1 GA-FGM, 6/14 differentiated adenocarcinoma). Conclusions Diagnostic limitations of M-NBI depend on four distinct histological characteristics. For accurate diagnosis of M-NBI-DLLs, it may be necessary to fully understand endoscopic features of these lesions using white light imaging and M-NBI based on these histological characteristics and to take a precise biopsy.
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Kim, Su Jin, Cheol Woong Choi, and Dae Hwan Kang. "Endoscopic features of submucosal invasion in undifferentiated type early gastric cancer sized less than 2 cm without ulceration." Journal of Clinical Oncology 37, no. 4_suppl (2019): 76. http://dx.doi.org/10.1200/jco.2019.37.4_suppl.76.
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76 Background: The prediction of invasion depth is important to decide the treatment modality for the undifferentiated type early gastric cancer (EGC) when size is less than 2 cm and had no ulceration. We aimed to identify the endoscopic features associated with submucosal invasion in the undifferentiated type EGC that meet the criteria of size and status of ulcer in the endoscopic submucosal dissection (ESD). Methods: A total of 120 patients with undifferentiated type EGC who received ESD or operation from August 2008 to December 2017 were enrolled in this study. All lesions met the ESD criteria except the invasion depth. We retrospectively reviewed endoscopic features of tumor before the resection and depth of invasion after resection. Results: In 120 undifferentiated EGCs, the mucosal and submucosal cancer were 97 and 23 lesions, respectively, In univariable analysis, discolor change, upper third location, the presence of deep/wide erosion were associated with submucosal invasion. Multivariable analysis revealed that upper/middle third location (odds ratio [OR] 8.0, 95% confidence interval [CI] 1.2-55.0; OR 7.9, 95% CI 1.8-35.1), erosion or polypoid (OR 41.8, 95% CI 4.1-427.9), and elevated type (OR 20.9, 95% CI 2.5-173.8) were significant risk factors. In 112 patients received gastrectomy with lymph nodes dissection, lymph node metastases were found in four cases (three mucosal cancer and one submucosal cancer). However, there was no lymph node metastasis in the lesions meeting the expanded ESD indication. Conclusions: The careful decision of treatment modality is needed for undifferentiated type EGC with erosion or elevated gross type located on the upper/middle third, although the tumor size and ulcer status meet the ESD indication.
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Taghavi, Seyed Mousa, Amir Shadboorestan, Samin Sabzevari, et al. "Protective Role of EGCG Against Malathion Induced Genotoxicity Using Human Lymphocytes." Drug Research 70, no. 08 (2020): 360–66. http://dx.doi.org/10.1055/a-1201-2436.
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AbstractBackground: Green tea (Camellia sinensis), which is the most common drink across the world after water, has many antioxidant properties. Epigallocatecin-3-gallate (EGCG) is a flavonoid which accounts for 33–50% of green tea solids. It functions as a powerful antioxidant, preventing oxidative damage in healthy cells, with antiangiogenic and antitumor activities and as a modulator of tumor cell response to chemotherapy. Malathion is an organophosphate pesticide which is widely used in agriculture, veterinary and industries. Oxidative stress has been identified as one of malathion’s main molecular mechanisms. The purpose of this study was to evaluate protective role of EGCG against malathion induced genotoxicity using human lymphocyte model. Blood samples from 8 non-smoker healthy volunteers with no history of chemotherapy were collected and divided into six groups: Control, EGCG (50 µM), EGCG (20 µM), Malathion (24 µM), EGCG (50 µM)+Malathion (24 µM) and EGCG (20 µM)+Malathion (24 µM). For genotoxicity assay, we employed micronuclei test. For antioxidant capacity evaluation, GSH content and MDA levels were measured. Malathion showed significant genotoxic damage compared to the intact lymphocytes, however, treatment with EGCG at both concentrations were reduced the genotoxic effect of malathion. Malathion induced lipid peroxidation, while pre-treatment with EGCG at both concentrations, significantly protected the lymphocytes against malathion induced lipid peroxidation. Malathion significantly reduced GSH content, but pre-treatment with EGCG significantly recovered GSH content. Overall this study demonstrated that EGCG (at both concentrations) significantly prevents human lymphocytes against malathion induced genotoxicity and oxidative damage.
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Horiguchi, Noriyuki, Tomomitsu Tahara, Tomohiko Kawamura, et al. "Distinct Clinic-Pathological Features of Early Differentiated-Type Gastric Cancers afterHelicobacter pyloriEradication." Gastroenterology Research and Practice 2016 (2016): 1–5. http://dx.doi.org/10.1155/2016/8230815.
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Background. Gastric cancer is discovered even after successful eradication ofH. pylori. We investigated clinic pathological features of early gastric cancers afterH. pylorieradication.Methods. 51 early gastric cancers (EGCs) from 44 patients diagnosed after successfulH. pylorieradication were included as eradication group. The clinic-pathological features were compared with that of 131 EGCs from 120 patients who did not have a history ofH. pylorieradication (control group).Results. Compared with control group, clinic-pathological features of eradication group were characterized as depressed (p<0.0001), reddish (p=0.0001), and smaller (p=0.0095) lesions, which was also confirmed in the comparison of six metachronous lesions diagnosed after initial ESD and subsequent successfulH. pylorieradication. Prevalence of both SM2 (submucosal invasion greater than 500 μm) and unexpected SM2 cases tended to be higher in eradication group (p=0.077, 0.0867, resp.). Prevalence of inconclusive diagnosis of gastric cancer during pretreatment biopsy was also higher in the same group (26.0% versus 1.6%,p<0.0001).Conclusions. Informative clinic pathological features of EGC afterH. pylorieradication are depressed, reddish appearances, which should be treated as a caution because histological diagnosis of cancerous tissue is sometimes difficult by endoscopic biopsy.
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Alimuddin, A. Octavera, O. Z. Arifin та K. Sumantadinata. "Characterization of β-Actin Promoter from Nile Tilapia (Oreochromis niloticus)". Jurnal Akuakultur Indonesia 7, № 2 (2008): 115. http://dx.doi.org/10.19027/jai.7.115-127.
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<p>Promoter is one of the factors determining the successful of transgenesis. In this study we isolated and characterized β-actin promoter from Nile tilapia (tiBP) towards production of autotransgenic tilapia. β-actin promoter has high activity in muscle. Sequence of tiBP promoter was isolated by using PCR method. Sequencing was performed using ABI PRISM 3100 machine. Analysis of sequences was conducted using GENETYX version 7 and TFBind softwares. DNA fragment of PCR amplification product digested from the vector cloning was then ligated with pEGFP-N1 to generate ptiBP-EGFP construct. The construct was microinjected into one-cell stage of zebrafish (<em>Danio rerio</em>) embryos to test the tiBP promoter activity. EGFP gene expression was observed by fluorescence microscope. The result of sequence analysis showed that the length of DNA fragment obtained is about 1.5 kb and containing the evolutionary conserved sequences of transcription factor for β-actin promoter including CCAAT, CArG and TATA boxes. Furthermore, tiBP sequence in ptiBP-EGFP construct could regulated GFP expression in muscle of zebrafish embryos injected with the construct. The results suggested that PCR amplification product is the regulator sequence of tilapia β-actin gene. Autotransgenic tilapia can be then produced by changing GFP gene fragment of ptiBP-EGFP construct with genes from tilapia encoding important traits in aquaculture.</p> <p>Keywords: cloning, β-actin promoter, autotransgenic, EGFP, <em>Oreochromis niloticus</em>, <em>Danio rerio</em></p> <p> </p> <p>ABSTRAK</p> <p>Promoter merupakan salah satu faktor penentu keberhasilan transgenesis. Pada penelitian ini kami mengisolasi dan mengkarakterisasi promoter β-actin dari ikan nila (tiBP) dalam rangka pembuatan ikan nila autotransgenik. Promoter β-actin memiliki aktivitas tinggi pada jaringan otot. Sekuens promoter tiBP diisolasi menggunakan metode PCR. Sekuensing dilakukan menggunakan mesin ABI PRISM 3100. Analisa sekuens menggunakan software GENETYX versi 7 dan TFBind. Fragment DNA hasil amplifikasi PCR yang didigesti dari vektor kloning selanjutnya diligasi dengan pEGFP-N1 untuk membuat konstruksi ptiBP-EGFP. Konstruksi ptiBP-EGFP dimikroinjeksi ke embrio ikan zebra (<em>Danio rerio</em>) fase 1 sel untuk menguji aktivitas promoter tiBP. Ekspresi gen EGFP diamati menggunakan mikroskop fluoresens. Analisa sekuens menunjukkan bahwa panjang fragmen DNA hasil amplifikasi PCR sekitar 1,5 kb dan memiliki faktor transkripsi yang konserf untuk promoter β-actin, yaitu CCAAT, boks CArG dan TATA. Selanjutnya, sekuens tiBP dalam konstruksi ptiBP-EGFP mampu mengendalikan ekspresi gen EGFP pada jaringan otot embrio ikan zebra yang dimikroinjeksi dengan konstruksi tersebut. Dengan demikian dapat disimpulkan bahwa fragmen DNA hasil amplifikasi PCR tersebut merupakan sekuens promoter β-actin ikan nila. Pembuatan ikan nila autotransgenik selanjutnya dapat dilakukan dengan mengganti gen EGFP pada pktBA-EGFP dengan gen-gen asal ikan nila yang mengkodekan karakter penting dalam budidaya ikan.</p> Kata kunci: kloning, promoter β-actin, autotransgenik, EGFP, <em>Oreochromis niloticus</em>, <em>Danio rerio</em>
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Chrenek, P., A. V. Makarevich, M. Bauer, and R. Jurcik. "Developmental rate and allocation of transgenic cells in rabbit chimeric embryos." Zygote 16, no. 1 (2008): 87–91. http://dx.doi.org/10.1017/s0967199407004534.
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SummaryThe objective of this study was to compare developmental capacity of rabbit chimeric embryos and the allocation of the EGFP gene expression to the embryoblast (ICM) or embryonic shield. We produced chimeric embryos (TR<>N) by synchronous transfer of two or three blastomeres at the 16-cell stage from transgenic (TR) into normal host embryos (N) at the same stage. In the control group, two to three non-transgenic blastomeres were used to produce chimeric embryos. The TR embryos were produced by microinjection of EGFP into both pronuclei of fertilized rabbit eggs. The developmental rate and allocation of EGFP-positive cells of the reconstructed chimeric embryos was controlled at blastocyst (96 h PC) and embryonic shield (day 6) stage.All chimeric embryos (120/120, 100%) developed up to blastocyst stage. Using fluorescent microscope, we detected green signal (EGFP expression). In 90 chimeric (TR<>N) embryos (75%). Average total number of cells in chimeric embryos at blastocyst stage was 175 ± 13.10, of which 58 ± 2.76 cells were found in the ICM area. The number of EGFP-positive cells in the ICM area was 24 ± 5.02 (35%). After the transfer of 50 chimeric rabbit embryos at the 16-cell stage, 20 embryos (40%) were flushed from five recipients on day 6 of pregnancy, of which five embryos (25%) were EGFP positive at the embryonic shield stage.Our results demonstrate that transgenic blastomeres in synchronous chimeric embryos reconstructed from TR embryos have an ability to develop and colonize ICM and embryonic shield area.
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Schuening, Friedrich G., Michail M. Zaboikin, Narasimhachar Srinivasakumar, and Tatiana N. Zaboikina. "Co-Epression of Two Transgenes in Lentiviral Vector Using Independent Transcriptional Units." Blood 104, no. 11 (2004): 5254. http://dx.doi.org/10.1182/blood.v104.11.5254.5254.
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Abstract There are several gene therapy approaches, which require transfer and co-expression of two transgenes within one target cell. To this end, we have created and tested two-gene expression HIV-1 based vectors, which encode enhanced green fluorescent protein (EGFP) and P144K mutant of canine O6-methylguanine-DNA-methyltransferase (MGMT) transgenes under either the phosphoglycerate kinase gene promoter (PGKp), or the elongation factor 1 alpha promoter (EF1a_p). Eight different configurations of the two transgene expression cassettes were created and tested within the same lentiviral backbone (see Figure). Individual VSV-G pseudotyped vectors stocks were produced and used for infection of canine thyroid adenocarcinoma (CTAC) cells at low multiplicity of infection (MOI = 0.1) to ensure 1 copy of proviral vector per transduced cell. The cells were harvested one week later and an aliquot was assayed for EGFP expression by flow cytometry. Another portion was subjected to selection with O6-benzylguanine (BG, 40 m M for 18 hrs) and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU, 100 m M for 2 hrs) and kept in culture for additional two weeks to eliminate cells expressing insufficient MGMT. The percentage of GFP positive cells, prior to selection with BG and BCNU, ranged between 0.1 % to 6.6% for the dual-transgene expression cassette encoding HIV-1 vectors. Following selection with BG and BCNU, the percentage of GFP positive cells increased for all vectors with the exception of vector #2 PGEM. Two of the vectors (#1 PMEG and #8 EGMP) demonstrated over 80% GFP positivity after selection. The results of flow cytometry after selection were corroborated by fluorescence microscopy of individual BCNU-resistant colonies. GFP expression was readily detected in drug-resistant colonies transduced with vectors #1 PMEG or #8 EGMP. Weaker GFP expression was detected in drug resistant cells transduced with vectors #3 PMGE, #5 EMPG or #6 EGPM. No significant GFP expression was observed in drug-resistant colonies transduced with vectors #2 PGEM, #4 PGME or #7 EMGP. Drug resistance to BCNU (IC50 values) provided by each of the vectors, was also determined. The data showed that the IC50 values for #1 PMEG and #8 EGMP vectors were 2.4-fold and 4.4-fold, respectively, higher than for mock transduced control cells. The above results indicate that coordinated co-expression of two transgenes using independent expression cassettes is promoter, position and orientation dependent. The data also indicate that two potentially useful vectors (#1 PMEG and #8 EGMP) have been identified for evaluation, ex vivo and in vivo, in the canine model for co-expression of two transgenes.
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Baker, Kimberly Mantzke, and Angela C. Bauer. "Green Tea Catechin, EGCG, Suppresses PCB 102-Induced Proliferation in Estrogen-Sensitive Breast Cancer Cells." International Journal of Breast Cancer 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/163591.
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The persistence of polychlorinated biphenyls (PCBs) in the environment is of considerable concern since they accumulate in human breast tissue and may stimulate the growth of estrogen-sensitive tumors. Studies have shown that EGCG from green tea can modify estrogenic activity and thus may act as a cancer chemopreventive agent. In the present study, we evaluated the individual and combined effects of PCB 102 and EGCG on cell proliferation using an estrogen-sensitive breast cancer cell line MCF-7/BOS. PCB 102 (1–10 μM) increased cell proliferation in a dose-dependent manner. Furthermore, the proliferative effects of PCB 102 were mediated by ERαand could be abrogated by the selective ERαantagonist MPP. EGCG (10–50 μM) caused a dose-dependent inhibition of PCB 102-induced cell proliferation, with nearly complete inhibition at 25 μM EGCG. The antiproliferative action of EGCG was mediated by ERβand could be blocked by the ERβ-specific inhibitor PHTPP. In conclusion, EGCG suppressed the proliferation-stimulating activity of the environmental estrogen PCB 102 which may be helpful in the chemoprevention of breast cancer.
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Cocucci, Emanuele, Raphaël Gaudin, and Tom Kirchhausen. "Dynamin recruitment and membrane scission at the neck of a clathrin-coated pit." Molecular Biology of the Cell 25, no. 22 (2014): 3595–609. http://dx.doi.org/10.1091/mbc.e14-07-1240.
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Dynamin, the GTPase required for clathrin-mediated endocytosis, is recruited to clathrin-coated pits in two sequential phases. The first is associated with coated pit maturation; the second, with fission of the membrane neck of a coated pit. Using gene-edited cells that express dynamin2-EGFP instead of dynamin2 and live-cell TIRF imaging with single-molecule EGFP sensitivity and high temporal resolution, we detected the arrival of dynamin at coated pits and defined dynamin dimers as the preferred assembly unit. We also used live-cell spinning-disk confocal microscopy calibrated by single-molecule EGFP detection to determine the number of dynamins recruited to the coated pits. A large fraction of budding coated pits recruit between 26 and 40 dynamins (between 1 and 1.5 helical turns of a dynamin collar) during the recruitment phase associated with neck fission; 26 are enough for coated vesicle release in cells partially depleted of dynamin by RNA interference. We discuss how these results restrict models for the mechanism of dynamin-mediated membrane scission.
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Cheng, Jinnian, Jie Xia, Qian Zhuang, et al. "A new exploration of white globe appearance (WGA) in ulcerative lesions." Zeitschrift für Gastroenterologie 58, no. 08 (2020): 754–60. http://dx.doi.org/10.1055/a-1200-2287.
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Abstract Aim White globe appearance (WGA), a small white lesion with a globular shape that can be clearly visualized by magnifying endoscopy with narrow-band imaging (ME-NBI), was reported to be a reliable marker of early gastric cancer (EGC). However, we found that this endoscopic presentation could also be seen in non-cancerous tissues, especially in ulcerative lesions. This study aimed to further investigate the diagnostic value of WGA in differentiating non-cancerous lesions from EGC in ulcer-type cases. Materials and Methods We retrospectively reviewed 54 cases of EGC and 155 cases of non-cancerous lesions in this study, all of which had endoscopic imaging data of ME-NBI scanning and pathological data of biopsy or resected specimens. The correlation of the prevalence of WGA and ulcerative lesions, as well as the characteristics of WGA between the 2 groups were analyzed in this study. Results WGA was more common in ulcerative lesions (27.6 %, 21/76) than in non-ulcerative lesions (3.8 %, 5/133) (p < 0.001) in our study. In the ulcerative cases, no significant difference in prevalence of WGA was observed between EGC and non-cancerous lesions (p = 0.532). Compared with WGA in EGC, WGA in non-cancerous lesions tended to show the characteristic of tree-branch-like vessels on globular shape (p < 0.001). Conclusions WGA is more likely to occur in ulcerative lesions, and the presence of WGA alone cannot distinguish EGC from non-cancerous lesions in ulcer-type cases. In WGA-positive tissue, tree-branch-like vessels of globular shape may provide a certain clinical value in diagnosis of non-cancerous lesions or EGC.
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AFIFY, Abd El-Moneim M. R., Emad A. SHALABY, and Hossam Saad EL-BELTAGI. "Antioxidant Activity of Aqueous Extracts of Different Caffeine Products." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39, no. 2 (2011): 117. http://dx.doi.org/10.15835/nbha3926254.
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The antioxidant activity of water extracts (cold and hot) of six caffeine products were carried out. The extracts were screened for total polyphenol contents and antioxidant activity using DPPH, ABTS methods and reducing power method at 50 and 100 μg/ml after 15 min and 30 min using DPPH, ABTS BHA and Caffeine as standard compounds. The results indicated that, the hot water extracts for different caffeine products showed higher antioxidant activity than those of cold extracts and this activity was time and concentration dependent. In addition, the activity was higher against ABTS radical more than DPPH and reducing power methods. Also, there is a positive correlation between the antioxidant and reducing compounds presented in water extracts of different caffeine products. The results of HPLC showed that fresh tea leaves are rich in flavanol monomers known as catechins. The most abundant catechin derivatives in green tea are EGC, EGCG and GC. On the other hand EGCG and GC are major catechin derivative in different caffeine product except El-Fakher tea and Cacao. Generally, these beverages had high antioxidant capacities and total phenolic contents, and could be important dietary sources of antioxidant phenolic for prevention of diseases caused by oxidative stress.
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Liu, Yanli, Mingkai Xu, Xu Li, Jian Sun, Chenggang Zhang, and Huiwen Zhang. "The construction of a bifunctional fusion protein consisting of SEC2 and EGFP." Biotechnology and Applied Biochemistry 61, no. 5 (2014): 565–71. http://dx.doi.org/10.1002/bab.1203.
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Koch, Wojciech, Wirginia Kukula-Koch, and Kazimierz Głowniak. "Catechin Composition and Antioxidant Activity of Black Teas in Relation to Brewing Time." Journal of AOAC INTERNATIONAL 100, no. 6 (2017): 1694–99. http://dx.doi.org/10.5740/jaoacint.17-0235.
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Abstract Black tea infusions are one of the most popular beverages across the world. Their extract composition depends on several factors, brewing time being one of the most important determinants. The aim of the present study was to determine the catechin composition of different black tea infusions using a validated LC electrospray ionization time-of-flight MS method. Additionally, total phenolic content (TPC) and antioxidant activity of infusions were evaluated using Folin–Ciocalteu reagent and stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). An optimized LC-MS method enabled the precise identification of the studied catechins [epicatechin (EC), EC gallate (ECG), epigallocatechin (EGC), and epigallocatechin-3-gallate (EGCG)] and gallic acid (GA). The major catechin in all investigated teas was EGC (25.6 mg/100 cm3 after 4 min of brewing). EC was present at the lowest concentration in all extracts. TPC and antiradical scavenging activity were in a good agreement with catechins and GA content. In general, the longer the brewing time, the higher the concentration of catechin, TPC, and antioxidant activity values. However, it should be noted that after 2 min brewing, most phenolics had already been extracted, and extract composition did not significantly change at a prolonged extraction time.
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Lazulva. "STUDI TEORITIS: STRUKTUR DAN SIFAT POLIFURAN TERSUBSTITUSI SEBAGAI SEMIKONDUKTOR DENGAN MENGGUNAKAN PROGRAM PM3." Photon: Jurnal Sain dan Kesehatan 2, no. 2 (2012): 25–32. http://dx.doi.org/10.37859/jp.v2i2.135.
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Telah dilakukan penelitian untuk mempelajari struktur dan sifat elektronik dari polimer furan dan turunannya (disubstitusi) dengan jumlah cincin furan n = 20 dengan menggunakan program PM3. Substituen yang digunakan dalam penelitian ini berupa atom atau molekul radikal pada posisi ujung. Subtituen yang dipelajari adalah fenil (Ph), radikal heksahalobenzena (-Z), radikal pirol (Py) dan tiofen (T).. Masukan data awal furan adalah sudut ikatan awal untuk C=C-C = 105˚, C=C-H = 120˚, H-C-C = 135˚, H-C-O = 123.872˚, O-C=C = 116˚, torsion angle = 0˚ dan panjang ikatan C=C, C-C = 1,4 Å, C-O = 1,408 Å dan C-H = 1 Å. Dalam penelitian ini digunakan komputasi secara RHF ( Restrical Hartree Fock) dengan pengoptimasi Polak Ribiere. Optimasi dilakukan dengan ketentuan berikut: spin multiplicity = 1; charge = 0; convergence limit = 0,001; iterration limit = 50; dan gradien 0,01 kCal/mol.Å. Luaran data (dalam keadaan optimal) yang dibutuhkan adalah Binding Energy (BE adalah energi ikatan antar atom-atom dalam molekul), EHOMO ( tingkat energi highest occupied molecular orbital dan ELUMO (tingkat energi lowest occupied molecular orbital). Sifat penghantar polimer ditentukan dari nilai ΔE atau (Egap). Nilai Egap suatu konduktor, semikonduktor dan isolator berturut-turut adalah < 1,0 eV; 1,0 – 3,0 eV; dan > 3 eV. Hasil pengoptimasian struktur turunan polifuran memperlihatkan bahwa seluruh substituen yang digunakan dapat menurunkan nilai band gap (Egap) polifuran. Nilai band gap (Egap) ternyata dipengaruhi oleh jumlah cincin furan yang berikatan membentuk polimer. Penurunan nilai band gap yang signifikan terjadi pada jumlah monomer furan n = 1 s.d 10.
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Sigon, Roberto, Vincenzo Canzonieri, Renato Cannizzaro, et al. "Early Gastric Cancer: Diagnosis, Surgical Treatment and Follow-Up of 45 Cases." Tumori Journal 84, no. 5 (1998): 547–51. http://dx.doi.org/10.1177/030089169808400507.
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Aims and background The 5-year survival rate of early gastric cancer (EGC) is 85%-100% after “curative” resection, as compared to 20%-30% in advanced gastric cancer (AGC). Because of this relatively high cure rate, the interest in the diagnosis and therapy of EGC has been steadily increasing. The present study, based on 45 EGCs, is aimed at a critical evaluation of the diagnostic procedures and surgical options. Methods and results Forty-five patients with early gastric cancer (27 men and 18 women; median age, 62 years; range, 28-84) were diagnosed and operated on. They represented 22.5% of all patients with gastric cancer (200) treated in the period July 1987 to January 1998. Forty-one patients were from the northeastern part of Italy. The most frequent symptom was epigastric pain (84%). Barium upper gastrointestinal radiography findings were strongly suggestive of malignancy in 41 cases (91%). Preoperative histopathological diagnosis of adenocarcinoma was performed in 43 cases (95.5%). In two cases (4.5%) severe epithelial dysplasia (associated with ulcer) was the first diagnosis, but the final diagnosis on the basis of the resected specimens was a well differentiated adenocarcinoma. The primary surgical procedure included i) subtotal distal resection (37 cases) with Billroth II (33) and Billroth I (4) reconstructions; ii) total gastrectomy (3) for proximal neoplastic extension; iii) proximal gastric resection (2) for cardial cancer; iv) degastro-total gastrectomy (3) for cancer of the stump. Two patients, previously treated with conservative surgery, underwent degastro-total gastrectomy for neoplastic microfocal extension to the margin of resection and for early anastomotic recurrence, respectively. Mural infiltration was limited to the mucosa and submucosa in 27 and 18 cases, respectively. Lymph node metastases were found in three mucosal and five submucosal tumor cases, involving either the first or the second echelon. No operative deaths or postsurgical complications occurred in this series. In the follow-up period (median, 36 months; range, 3-120) four patients died due to other causes; one developed liver metastases, another developed oropharyngeal cancer and two died of biopsy-proven lung cancer without evidence of gastric cancer recurrence. Conclusions The clinical presentation of EGC is aspecific. Preoperative endoscopy with biopsy remains the most sensitive diagnostic procedure. For treatment, subtotal distal gastric resection with lymphadenectomy is the “gold standard” but in some instances total gastrectomy may be indicated. Accurate pathological examination establishes the depth of infiltration, as well as the superficial extension of tumors and the lymph node status. Although the prognosis of EGC is favorable, a medium-term follow-up should be planned.
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de Morais Junior, Alcides C., Raquel M. Schincaglia, Marisa Passarelli, Gustavo D. Pimentel, and João F. Mota. "Acute Epigallocatechin-3-Gallate Supplementation Alters Postprandial Lipids after a Fast-Food Meal in Healthy Young Women: A Randomized, Double-Blind, Placebo-Controlled Crossover Study." Nutrients 12, no. 9 (2020): 2533. http://dx.doi.org/10.3390/nu12092533.
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A high-fat fast-food meal negatively impacts postprandial metabolism even in healthy young people. In experimental studies, epigallocatechin-3-gallate (EGCG), a bioactive compound present in green tea, has been described as a potent natural inhibitor of fatty acid synthase. Thus, we sought to evaluate the effects of acute EGCG supplementation on postprandial lipid profile, glucose, and insulin levels following a high-fat fast-food meal. Fourteen healthy young women 21 ± 1 years and body mass index 21.4 ± 0.41 kg/m2 were enrolled in a randomized, double-blind, placebo-controlled crossover study. Participants ingested capsules containing 800 mg EGCG or placebo immediately before a typical fast-food meal rich in saturated fatty acids. Blood samples were collected at baseline and then at 90 and 120 min after the meal. The EGCG treatment attenuated postprandial triglycerides (p = 0.029) and decreased high-density lipoprotein cholesterol (HDL-c) (p = 0.016) at 120 min. No treatment × time interaction was found for total cholesterol, low-density lipoprotein (LDL-c), and glucose or insulin levels. The incremental area under the curve (iAUC) for glucose was decreased by EGCG treatment (p < 0.05). No difference was observed in the iAUC for triglycerides and HDL-c. In healthy young women, acute EGCG supplementation attenuated postprandial triglycerides and glucose but negatively impacted HDL-c following a fast-food meal.
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Moro, L. N., G. Vichera, and D. Salamone. "240 QUALITY AND VIABILITY OF IVF BOVINE EMBRYOS AFTER INTRACYTOPLASMIC INJECTION OF DNA–LIPOSOME COMPLEXES." Reproduction, Fertility and Development 24, no. 1 (2012): 232. http://dx.doi.org/10.1071/rdv24n1ab240.
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Transgenic animals have important applications in agriculture and human medicine; nevertheless the available techniques still remain inefficient and technically difficult. We have recently developed a novel method to transfect bovine embryos that consists of intracytoplasmic injection of exogenous DNA–liposome complexes (eDNA-LC) in IVF zygotes. This study was designed to evaluate the quality and viability of IVF bovine embryos, after intracytoplasmic injection of pCX-EGFP–liposome complexes (EGFP-LC) or pBCKIP2.8-liposome complexes (plasmid that codifies the human insulin gene, HI-LC). First, we evaluated embryo development and enhanced green fluorescent protein (EGFP) expression of IVF embryos injected with both plasmids separately. This treatment was analysed by Fisher's Exact test (P ≤ 0.05). Cleavage rates for EGFP-LC, HI-LC and IVF embryos injected with liposomes alone (IVF-L) and IVF control (IVF-C) were 62% (63/102), 67% (67/100), 66% (67/101) and 79% (98/124); blastocysts rates were 17% (17/102), 21% (21/100), 21% (21/101) and 23% (28/124), respectively. No statistical differences were seen among groups. The percentage of EGFP-positive embryos (EGFP+) after EGFP-LC injection was 42.9% after 3 days of culture and 41.8% at the blastocyst stage. In the second experiment, the blastocysts obtained, EGFP+ or EGFP-negative (EGFP–), were analysed by TUNEL assay at Day 6 (Bd6), 7 (Bd7) and 8 (Bd8) of in vitro culture, in order to evaluate the effect of the transgene and culture length, on DNA fragmentation. This treatment was analysed by the difference of proportions test (P ≤ 0.05) using statistical INFOSTAT software. All EGFP+ blastocysts showed TUNEL positive cells (T+). The percentage of T+ in Bd6, Bd7 and Bd8 were 91, 73.7 and 99.5%, respectively (P ≤ 0.05). EGFP– blastocysts showed lower fragmented nuclei (0, 44.6 and 85%, respectively; P ≤ 0.05). Groups IVF-L and IVF-C were also evaluated. In both groups, there was no evidence of DNA fragmentation in Bd6 and Bd7, but T+ were detected in Bd8 (66.4 and 85.8%, respectively; P ≤ 0.05). In the third experiment, bovine blastocysts obtained from the HI-LC group were individually transferred to recipient cows after 6 (n = 11), 7 (n = 5) and 8 (n = 5) days of culture post-IVF and HI-LC injection. The pregnancies obtained were from Bd6 [18.2% (2/11)] and Bd7 [40% (2/5)], although none of the recipients receiving Bd8 were diagnosed pregnant. Two pregnancies developed to term, one derived from Bd6 and the other from Bd7. Analysis by PCR determined that none of the born cows were transgenic. In summary, IVF bovine embryos could be easily transfected after the injection of eDNA-LC and the technique did not affect offspring viability. The results indicate that extended time in in vitro culture increases the percentage of fragmented nuclei in blastocysts. Moreover, this parameter increases in blastocysts with transgene expression compared with those without expression. Finally, more transfers are required in order to obtain the real efficiency of this new technique and to overcome the drawbacks generated by in vitro culture length and transgene expression.
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Biddick, Kathleen. "Fiction and History in England, 1066–1200 (review)." JEGP, Journal of English and Germanic Philology 108, no. 2 (2009): 257–58. http://dx.doi.org/10.1353/egp.0.0027.
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Bulboaca, Adriana Elena, Paul-Mihai Boarescu, Alina Silvia Porfire, et al. "The Effect of Nano-Epigallocatechin-Gallate on Oxidative Stress and Matrix Metalloproteinases in Experimental Diabetes Mellitus." Antioxidants 9, no. 2 (2020): 172. http://dx.doi.org/10.3390/antiox9020172.
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Background: The antioxidant properties of epigallocatechin-gallate (EGCG), a green tea compound, have been already studied in various diseases. Improving the bioavailability of EGCG by nanoformulation may contribute to a more effective treatment of diabetes mellitus (DM) metabolic consequences and vascular complications. The aim of this study was to test the comparative effect of liposomal EGCG with EGCG solution in experimental DM induced by streptozotocin (STZ) in rats. Method: 28 Wistar-Bratislava rats were randomly divided into four groups (7 animals/group): group 1—control group, with intraperitoneal (i.p.) administration of 1 mL saline solution (C); group 2—STZ administration by i.p. route (60 mg/100 g body weight, bw) (STZ); group 3—STZ administration as before + i.p. administration of EGCG solution (EGCG), 2.5 mg/100 g b.w. as pretreatment; group 4—STZ administration as before + i.p. administration of liposomal EGCG, 2.5 mg/100 g b.w. (L-EGCG). The comparative effects of EGCG and L-EGCG were studied on: (i) oxidative stress parameters such as malondialdehyde (MDA), indirect nitric oxide (NOx) synthesis, and total oxidative status (TOS); (ii) antioxidant status assessed by total antioxidant capacity of plasma (TAC), thiols, and catalase; (iii) matrix-metalloproteinase-2 (MMP-2) and -9 (MMP-9). Results: L-EGCG has a better efficiency regarding the improvement of oxidative stress parameters (highly statistically significant with p-values < 0.001 for MDA, NOx, and TOS) and for antioxidant capacity of plasma (highly significant p < 0.001 for thiols and significant for catalase and TAC with p < 0.05). MMP-2 and -9 were also significantly reduced in the L-EGCG-treated group compared with the EGCG group (p < 0.001). Conclusions: the liposomal nanoformulation of EGCG may serve as an adjuvant therapy in DM due to its unique modulatory effect on oxidative stress/antioxidant biomarkers and MMP-2 and -9.
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Liu, Hang-Seng, Yen-Hang Chen, Pei-Fang Hung, and Yung-Hsi Kao. "Inhibitory effect of green tea (−)-epigallocatechin gallate on resistin gene expression in 3T3-L1 adipocytes depends on the ERK pathway." American Journal of Physiology-Endocrinology and Metabolism 290, no. 2 (2006): E273—E281. http://dx.doi.org/10.1152/ajpendo.00325.2005.
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Resistin (Rstn) is known as an adipocyte-specific secretory hormone that can cause insulin resistance and decrease adipocyte differentiation. By contrast, green tea catechins, especially (−)-epigallocatechin gallate (EGCG), have been reported as body weight and diabetes chemopreventatives. Whether EGCG regulates production of Rstn is unknown. Using 3T3-L1 adipocytes, we found that EGCG at 20 and 100 μM suppressed Rstn mRNA levels by ∼35 and 50%, respectively, after 3 h. The basal half-life of Rstn mRNA induced by actinomycin D was >12 h but shifted to 3 h in the presence of EGCG. This suggests that EGCG regulates the stability of Rstn mRNA. Treatment with cycloheximide did not prevent EGCG-suppressed Rstn mRNA levels, which suggests that the effect of EGCG does not require new protein synthesis. Intracellular Rstn protein significantly decreased in the presence of 100 μM EGCG 3 h after treatment, whereas the release of the Rstn protein did not significantly change. This suggests that EGCG may modulate the distribution of Rstn protein between the intracellular and extracellular compartments. EGCG did not affect the amounts of extracellular signal-related kinase-1/2 (ERK1/2), phospho-JNK, phospho-p38, and phospho-Akt proteins but reduced the amounts of phospho-ERK1/2 proteins. Overexpression with MEK1 blocked EGCG-inhibited Rstn mRNA expression. These data suggest that EGCG downregulates Rstn expression via a pathway that is dependent on the ERK pathway.
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Barquinero, Jordi, José Carlos Segovia, Manuel Ramı́rez, et al. "Efficient transduction of human hematopoietic repopulating cells generating stable engraftment of transgene-expressing cells in NOD/SCID mice." Blood 95, no. 10 (2000): 3085–93. http://dx.doi.org/10.1182/blood.v95.10.3085.
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Abstract In an attempt to develop efficient procedures of human hematopoietic gene therapy, retrovirally transduced CD34+ cord blood cells were transplanted into NOD/SCID mice to evaluate the repopulating potential of transduced grafts. Samples were prestimulated on Retronectin-coated dishes and infected with gibbon ape leukemia virus (GALV)-pseudotyped FMEV vectors encoding the enhanced green fluorescent protein (EGFP). Periodic analyses of bone marrow (BM) from transplanted recipients revealed a sustained engraftment of human hematopoietic cells expressing the EGFP transgene. On average, 33.6% of human CD45+ cells expressed the transgene 90 to120 days after transplantation. Moreover, 11.9% of total NOD/SCID BM consisted of human CD45+ cells expressing the EGFP transgene at this time. The transplantation of purified EGFP+ cells increased the proportion of CD45+ cells positive for EGFP expression to 57.7% at 90 to 120 days after transplantation. At this time, 18.9% and 4.3% of NOD/SCID BM consisted of CD45+/EGFP+ and CD34+/EGFP+ cells, respectively. Interestingly, the transplantation of EGFP− cells purified at 24 hours after infection also generated a significant engraftment of CD45+/EGFP+ and CD34+/EGFP+ cells, suggesting that a number of transduced repopulating cells did not express the transgene at that time. Molecular analysis of NOD/SCID BM confirmed the high levels of engraftment of human transduced cells deduced from FACS analysis. Finally, the analysis of the provirus insertion sites by conventional Southern blotting indicated that the human hematopoiesis in the NOD/SCID BM was predominantly oligoclonal.
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Barquinero, Jordi, José Carlos Segovia, Manuel Ramı́rez, et al. "Efficient transduction of human hematopoietic repopulating cells generating stable engraftment of transgene-expressing cells in NOD/SCID mice." Blood 95, no. 10 (2000): 3085–93. http://dx.doi.org/10.1182/blood.v95.10.3085.010k01_3085_3093.
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In an attempt to develop efficient procedures of human hematopoietic gene therapy, retrovirally transduced CD34+ cord blood cells were transplanted into NOD/SCID mice to evaluate the repopulating potential of transduced grafts. Samples were prestimulated on Retronectin-coated dishes and infected with gibbon ape leukemia virus (GALV)-pseudotyped FMEV vectors encoding the enhanced green fluorescent protein (EGFP). Periodic analyses of bone marrow (BM) from transplanted recipients revealed a sustained engraftment of human hematopoietic cells expressing the EGFP transgene. On average, 33.6% of human CD45+ cells expressed the transgene 90 to120 days after transplantation. Moreover, 11.9% of total NOD/SCID BM consisted of human CD45+ cells expressing the EGFP transgene at this time. The transplantation of purified EGFP+ cells increased the proportion of CD45+ cells positive for EGFP expression to 57.7% at 90 to 120 days after transplantation. At this time, 18.9% and 4.3% of NOD/SCID BM consisted of CD45+/EGFP+ and CD34+/EGFP+ cells, respectively. Interestingly, the transplantation of EGFP− cells purified at 24 hours after infection also generated a significant engraftment of CD45+/EGFP+ and CD34+/EGFP+ cells, suggesting that a number of transduced repopulating cells did not express the transgene at that time. Molecular analysis of NOD/SCID BM confirmed the high levels of engraftment of human transduced cells deduced from FACS analysis. Finally, the analysis of the provirus insertion sites by conventional Southern blotting indicated that the human hematopoiesis in the NOD/SCID BM was predominantly oligoclonal.
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Hafez, E., and L. Geries. "Onion (Allium cepa L.) Growth, Yield and Economic Return Under Different Combinations of Nitrogen Fertilizers and Agricultural Biostimulants." Cercetari Agronomice in Moldova 51, no. 3 (2018): 69–88. http://dx.doi.org/10.2478/cerce-2018-0026.
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Abstract Two field experiments have been conducted to study the effects of application of nitrogen fertilizer, bio-fertilizers and organic compounds on growth, yield and economic of onion production in 2014/2015 and 2015/2016 seasons. From the data it was found that combination of N fertilization of onion plants with 100 kg N fed.−1 (hectare = 2.38 feddan) and foliar with humic acid at the rate of 1 kg fed.−1 is the best in this study, for giving the highest bulb yield with the highest net returns of 12580 EGP (1USD = 17.80 EGP), with a benefit: cost ratio (B:C ratio) of 2.35. While, the highest cost of cultivation was obtained by 120 kg N fed.−1 and spraying onion plants with humic acid followed by compost tea. Also, from the economic view, the revenue of EGP is higher when used some biofertilizers and organic fertilizers if compared with chemical fertilization only.
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Han, Dong-Wook, Mi Hee Lee, Bongju Kim, Jun Jae Lee, Suong-Hyu Hyon, and Jong-Chul Park. "Preventive Effects of Epigallocatechin-3-O-Gallate against Replicative Senescence Associated with p53 Acetylation in Human Dermal Fibroblasts." Oxidative Medicine and Cellular Longevity 2012 (2012): 1–13. http://dx.doi.org/10.1155/2012/850684.
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Considering the various pharmacological activities of epigallocatechin-3-O-gallate (EGCG) including anticancer, and anti-inflammatory, antidiabetic, and so forth, relatively less attention has been paid to the antiaging effect of EGCG on primary cells. In this study, the preventive effects of EGCG against serial passage-induced senescence were investigated in primary cells including rat vascular smooth muscle cells (RVSMCs), human dermal fibroblasts (HDFs), and human articular chondrocytes (HACs). The involvement of Sirt1 and acetylated p53 was examined as an underlying mechanism for the senescence preventive activity of EGCG in HDFs. All cells were employed with the initial passage number (PN) between 3 and 7. For inducing senescence, the cells were serially passaged at the predetermined times and intervals in the absence or presence of EGCG (50 or 100 μM). Serial passage-induced senescence in RVSMCs and HACs was able to be significantly prevented at 50 μM EGCG, while in HDFs, 100 μM EGCG could significantly prevent senescence and recover their cell cycle progression close to the normal level. Furthermore, EGCG was found to prevent serial passage- and H2O2-induced senescence in HDFs by suppressing p53 acetylation, but the Sirt1 activity was unaffected. In addition, proliferating HDFs showed similar cellular uptake of FITC-conjugated EGCG into the cytoplasm with their senescent counterparts but different nuclear translocation of it from them, which would partly account for the differential responses to EGCG in proliferating versus senescent cells. Taking these results into consideration, it is suggested that EGCG may be exploited to craft strategies for the development of an antiaging or age-delaying agent.
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Matsumura, Kazuaki, Hiroshi Takayama, Jung Yoon Bae, Mitsuru Kurihara, Sadami Tsutsumi, and Suong-Hyu Hyon. "Preservation of Platelets by Adding Epigallocatechin-3-O-Gallate to Platelet Concentrates." Cell Transplantation 18, no. 5-6 (2009): 521–28. http://dx.doi.org/10.1177/096368970901805-606.
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The effect of epigallocatechin-3- O-gallate (EGCG), a major component of green tea, on platelet preservation was evaluated. Single donor platelets ( N = 10) were collected and preserved by the standard method. EGCG was added to the platelet concentrates before preservation and then the functional and biochemical parameters were monitored throughout the storage period. After 6 days of preservation, the aggregability of the platelets was significantly maintained by addition of 50 and 100 μg/ml of EGCG. Platelet prothrombinase activity was also significantly retained by the addition of EGCG. The accumulation of P-selectin and RANTES in the plasma preserved with EGCG was less than those preserved without EGCG, which indicated that EGCG might inhibit platelet activation. Furthermore, EGCG reduced the increase of LDH in plasma during preservation and inhibited the activation of caspase-3 and cleavage of gelsolin, thereby showing that EGCG could inhibit the apoptosis of platelets. These results suggest that EGCG may play an effective role in preserving platelets by inhibiting the activation and apoptosis of platelets.
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Chang, Chi-Wu, Yi-Hsien Hsieh, Wei-En Yang, Shun-Fa Yang, Yueqin Chen, and Dan-Ning Hu. "Epigallocatechingallate Inhibits Migration of Human Uveal Melanoma Cells via Downregulation of Matrix Metalloproteinase-2 Activity and ERK1/2 Pathway." BioMed Research International 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/141582.
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The effects of epigallocatechingallate (EGCG) on the migration and expression of MMP-2 of uveal melanoma cells have not been reported. We studied this effect and relevant signaling pathways in a human uveal melanoma cell line (M17). MTT study found that EGCG did not affect the cell viability of M17 cells up to 100 µM. Wound-healing assay showed that EGCG significantly reduced the migration of melanoma cells in a dose-dependent manner from 20 to 100 µM. Gelatin zymography showed that secreted MMP-2 activity was dose-dependently inhibited by EGCG, whereas the MMP-2 expression at protein and mRNA levels was not affected as determined by western blot and RT-PCR analysis. EGCG significantly increased the expressions of MMP-2 endogenous inhibitors (TIMP-2 and RECK) in M17 cells. Western blot analysis of MAPK signal pathways showed that EGCG significantly decreased phosphorylated ERK1/2 levels, but not p38 and JNK levels, in melanoma cells. ERK1/2 inhibitors also reduced the migration and activity of MMP-2 in M17 cells. The present study suggested EGCG at nontoxic levels could inhibit migration of melanoma cells via downregulation of activities of secreted MMP-2 through the inhibition of the ERK1/2 phosphorylation. Therefore, EGCG may be a promising agent to be explored for the prevention of metastasis of uveal melanoma.
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Moreno-Vásquez, María J., Maribel Plascencia-Jatomea, Saúl Sánchez-Valdes, et al. "Characterization of Epigallocatechin-Gallate-Grafted Chitosan Nanoparticles and Evaluation of Their Antibacterial and Antioxidant Potential." Polymers 13, no. 9 (2021): 1375. http://dx.doi.org/10.3390/polym13091375.
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Nanoparticles based on chitosan modified with epigallocatechin gallate (EGCG) were synthetized by nanoprecipitation (EGCG-g-chitosan-P). Chitosan was modified by free-radical-induced grafting, which was verified by Fourier transform infrared (FTIR). Furthermore, the morphology, particle size, polydispersity index, and zeta potential of the nanoparticles were investigated. The grafting degree of EGCG, reactive oxygen species (ROS) production, antibacterial and antioxidant activities of EGCG-g-chitosan-P were evaluated and compared with those of pure EGCG and chitosan nanoparticles (Chitosan-P). FTIR results confirmed the modification of the chitosan with EGCG. The EGCG-g-chitosan-P showed spherical shapes and smoother surfaces than those of Chitosan-P. EGCG content of the grafted chitosan nanoparticles was 330 μg/g. Minimal inhibitory concentration (MIC) of EGCG-g-chitosan-P (15.6 μg/mL) was lower than Chitosan-P (31.2 μg/mL) and EGCG (500 μg/mL) against Pseudomonas fluorescens (p < 0.05). Additionally, EGCG-g-chitosan-P and Chitosan-P presented higher Staphylococcus aureus growth inhibition (100%) than EGCG at the lowest concentration tested. The nanoparticles produced an increase of ROS (p < 0.05) in both bacterial species assayed. Furthermore, EGCG-g-chitosan-P exhibited higher antioxidant activity than that of Chitosan-P (p < 0.05) in 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and ferric-reducing antioxidant power assays. Based on the above results, EGCG-g-chitosan-P shows the potential for food packaging and biomedical applications.
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Handayani, Ririn. "Ekstrak Teh Hijau Meningkatkan Ekspresi ER-alfa pada Endometrium dan Tuba Falopi Tikus Betina Yang Dipapar Sipermetrin." Jurnal Kebidanan 7, no. 2 (2018): 103. http://dx.doi.org/10.26714/jk.7.2.2018.103-110.
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Infertilitas banyak terjadi pada wanita karena gangguan sistem reproduksi yang bisa disebabkan oleh pestisida (sipermentrin). Paparan sipermetrin dapat memberi dampak pada otak yang menyebabkan kerusakan hipotalamus melalui proses stres oksidatif. Kemudian dapat mempengaruhi sekresi dari GnRH, LH dan FSH pada proses umpan balik hormon endogen. Sehingga akan mempengaruhi proses folikulogenesis dan menghasilkan estrogen sedikit. Estrogen ini akan menstimulasi pertumbuhan sel jika berikatan dengan reseptornya (ER-α). Teh hijau dengan kandungan antioksidan yang tinggi mampu menghambat radikal bebas. Jenis flavonol dari teh hijau umumnya dikenal sebagai catechin yaitu EGCG. EGCG bekerja sama dengan antioksidan endogen untuk melindungi tubuh dari reaksi stres oksidatif. Tujuan dari penelitian ini untuk membuktikan pemberian ekstrak teh hijau dapat meningkatkan ekspresi ER-α pada endometrium dan tuba falopi tikus betina yang dipapar sipermetrin. Penelitian ini merupakan penelitian eksperimental. Proses ekstraksi dengan metode maserasi. Pemeriksaan ekpresi ER-α dengan metode imunohistokimia (IHK). Data dianalisis menggunakan uji Rank Spearman. Hasil uji statistik didapatkan nilai korelasi 0,776 untuk ekspresi ER-α pada endometrium dan 0,718 untuk ekspresi ER-α pada tuba falopi. Berdasarkan hasil penelitian dapat disimpulkan bahwa ada hubungan positif searah dan cukup kuat antara pemberian ekstrak teh hijau dengan ekspresi ER-α pada endometrium dan tuba falopi. Semakin tinggi dosis yang diberikan semakin tinggi ekspresi yang dihasilkan
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Yang, Xiu Hong, Yu Pan, Xiao Li Zhan, Bao Long Zhang, Li Li Guo, and Hui Min Jin. "Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice." Oxidative Medicine and Cellular Longevity 2016 (2016): 1–14. http://dx.doi.org/10.1155/2016/2968462.
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Epigallocatechin-3-gallate (EGCG), extracted from green tea, has been shown to have antioxidative activity. In the present study, we evaluated the effect of EGCG on the kidney function in db/db mice and also tried to investigate the underlying mechanism of the renoprotective effects of EGCG in both animals and cells. EGCG treatment could decrease the level of urinary protein, 8-iso-PGF2a, and Ang II. Moreover, EGCG could also change the level of several parameters associated with oxidative stress. In addition, the protein expression levels of AT-1R, p22-phox, p47-phox, p-ERK1/2, p-p38 MAPK, TGF-β1, andα-SMA in diabetic db/db mice were upregulated, and all of these symptoms were downregulated with the treatment of EGCG at 50 and 100 mg/kg/d. Furthermore, the pathological changes were ameliorated in db/db mice after EGCG treatment. HK-2 cell-based experiments indicated that EGCG could inhibit the expression of MAPK pathways, which is the downstream effector of Ang II mediated oxidative stress. All these results indicated that EGCG treatment could ameliorate changes of renal pathology and delay the progression of DKD by suppressing hyperglycemia-induced oxidative stress in diabetic db/db mice.
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Hou, Huimin, Ying Wang, Chunshi Li, Jian Wang, and Yanli Cao. "Dipeptidyl Peptidase-4 Is a Target Protein of Epigallocatechin-3-Gallate." BioMed Research International 2020 (February 11, 2020): 1–9. http://dx.doi.org/10.1155/2020/5370759.
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Epigallocatechin-3-gallate (EGCG), a major active ingredient in green tea, has various health benefits. It affects glucose metabolism, but the mechanism is not well understood. This study aimed to identify targets of EGCG related to glucose metabolism. The core fragment of EGCG is a flavonoid. The flavonoid scaffold was used as a substructure to find proteins cocrystallized with flavonoids in the Protein Data Bank. The proteins identified were screened in PubMed for known relationships with diabetes. Dipeptidyl peptidase-4 (DPP4; PDB 5J3J) was identified following this approach. By molecular docking, the interactions of EGCG and DPP4 were assessed. To test the stability of the interactions between EGCG and DPP4, molecular dynamics simulation for 100 ns was performed using Desmond software. In vitro, the concentration of EGCG required to inhibit DPP4 activity by 50% (the IC50 value) was 28.42 μM. These data provide a theoretical basis for intervention in glucose metabolism with EGCG.
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Mohd Zin, Zamzahaila, Nursyafiqah Mohamad, Chong Kah Hui, Nurul Izwanie Majid, and Mohd Khairi Zainol. "Effect of Acidified Ethanol on Antioxidant Properties of Morinda citrifolia Leaf Extract and Its Catechin Derivatives." Current Research in Nutrition and Food Science Journal 9, no. 1 (2021): 172–83. http://dx.doi.org/10.12944/crnfsj.9.1.17.
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This study was conducted to investigate the effect of ethanol acidification on the antioxidant properties of Morinda citrifolia leaf (MCL) extract and its catechin derivatives. Four different ethanol (100%, 99.5%, 70%, 50%) with or without 0.5% acetic acid were used for extraction. The antioxidant profile was studied with DPPH radical scavenging activity, FRAP and TPC. The quantification of catechins in MCL was performed using HPLC, and the identification of catechins derivatives was performed with Ultra UPLC-TWIMS-QTOF. The results showed that an extraction solvent composed of 70% ethanol: 29.5% water: 0.5 % acetic acid exhibited the highest DPPH percentage of inhibition (86.12±2.96%) and highest TPC value with 97.80±0.25 mg GAE/g extract, while 100% ethanol acidified with 0.5% acetic acid showed highest FRAP antioxidant power with 1.31±0.05mg FSE/g extract. All eight types of catechins were identified in MCL and the most total catechins were quantified in 70% ethanol: 29.5% water: 0.5 % acetic acid at 153.57mg/g. The catechin derivatives identified included epigallocatechin-3-O-gallate (EGCG), epigallocatechin (4β, 8)-gallocatechin, gallocatechin (4α→8)-epicatechin, catechin-3-O-gallate (CG) and epigallocatechin (EGC). The results suggest that acidification improves the extraction of polyphenols as well as catechin content.
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Duan, Zhou Wei, Zhi Hao Dou, Hui Xie, Ai He, and Zhu Ning Wan. "Preparation of the Scales Material Adsorption EGCG." Advanced Materials Research 960-961 (June 2014): 270–73. http://dx.doi.org/10.4028/www.scientific.net/amr.960-961.270.
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Tilapia scale was used as the raw material to explore the preparation methods of EGCG adsorption material. Based on the results, the most reasonable interpretation of the data indicates that the EGCG adsorption material preparation process was as follows, particle size 0.30-0.45 mm , preparation temperature 110°C,pH 7 , solid to liquid ratio (g/mL) 1:6, time 20 min . Under this condition, the adsorption capacity of EGCG was 23.53mg/g.
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Fang, Changge, Caiqiao Zhang, Guoliang Xia, and Wei Yang. "Damaging effects of polychlorinated biphenyls on chicken primordial germ cells." Reproduction, Fertility and Development 14, no. 3 (2002): 177. http://dx.doi.org/10.1071/rd01118.
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This work describes the effects of a commercial polychlorinated biphenyl (PCB) mixture, Aroclor 1254, as well as 17β-oestradiol (E2) and testosterone on numbers and histomorphological changes of primordial germ cells (PGCs) in gonadal regions of Day 5 Hyline chicken embryos. The oestrogen receptor antagonist, clomiphen, alone or with PCBs was used in an attempt to protect the developing gonad from oestrogen-like effects of chemical PCBs. The results were as follows: (i) PCBs delayed embryonic development independently of dose (1�µg/eggP<0.05; 10 µg/eggP<0.01; 100 µg/eggP<0.001 v. the control) and caused a dose-independent increase in mortality compared with the control group (10 µg/egg P<0.01; 100 µg/eggP<0.05); maximal mortality was observed in the 1 µg/eggP<0.001); (ii) PCBs decreased PGC numbers dose dependently (P<0.001) and caused a swollen nucleus with hyperchromatism (pyknosis) or cytoplasm vacuolation as signs of gonadal PGC degeneration in all PCB groups, or even complete disappearance in the 100 µg/eggiii) after PCB treatment, the index of gonadal lesion increased significantly with the decrease of gonadal PGC number (1, 10 and 100 µg/eggP<0.001); (iv) there were no observed effects of E2, testosterone and clomiphen on PGCs in the experiments; and (v) clomiphen failed to block the damaging effects of PCBs. These results suggest that the adverse effects of PCBs on chicken gonadal and germ cell development were initiated during the early stages of incubation through direct toxic effects, rather than through oestrogen-mimicking actions. As PGC numbers in the gonads decrease and the index of gonadal lesion increases, one may expect reproductive function to be compromised.
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Lao, Weiguo, Yi Tan, Xingliang Jin, Linda Xiao, Jane J. Y. Kim, and Xianqin Qu. "Comparison of Cytotoxicity and the Anti-Adipogenic Effect of Green Tea Polyphenols with Epigallocatechin-3-Gallate in 3T3-L1 Preadipocytes." American Journal of Chinese Medicine 43, no. 06 (2015): 1177–90. http://dx.doi.org/10.1142/s0192415x15500676.
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Recent studies have demonstrated the effects of green tea polyphenols (GTP) and epigallocatechin-3-gallate (EGCG) on obesity. However, high doses of EGCG have also exhibited cytotoxicity. The aim of this study was to compare total GTP with purified EGCG on cytotoxicity, and to investigate the effects and the molecular mechanism of total GTP and EGCG on adipogenesis. Cytotoxicity was determined by cell viability assay. For the adipogenesis study, 3T3-L1 preadipocytes were incubated with three doses of GTP (1, 10, and 100 μg/ml) and the effect of EGCG (6.8 μg/ml) was compared with 10 μg/ml GTP containing 68% EGCG. Oil Red O staining and triglyceride content assay were carried out 10 days after differentiation and treatment. Adipogenic regulators CCAAT element binding protein α (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ) and sterol regulatory element-binding protein-1c (SREBP-1c) were determined by qRT-PCR and immunoblotting. GTP at 1000 μg/ml and EGCG (68 and 680 μg/ml) significantly affected cell viability. Purified EGCG had greater cytotoxicity than corresponding doses of GTP. About 10 μg/ml of GTP showed stronger reduction in triglyceride accumulation than EGCG treatment. Transcriptional factors of C/EBPα, SREBP-1c and PPARγ were markedly decreased in both GTP and EGCG-treated cells and GTP exhibited stronger inhibitory effects on C/EBPα and PPARγ protein expression than EGCG (p < 0.05). In conclusion, total GTP exerted greater inhibitory effects than purified EGCG on adipogenesis through down-regulating the adipogenic factor C/EBPα, SREBP-1c and PPARγ expression. These findings support that a polyphenol mixture is safer and more effective than EGCG alone for preventing obesity and obesity-related chronic diseases.
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Zhao, Wei-Hua, Zhi-Qing Hu, Sachie Okubo, Yukihiko Hara та Tadakatsu Shimamura. "Mechanism of Synergy between Epigallocatechin Gallate and β-Lactams against Methicillin-Resistant Staphylococcus aureus". Antimicrobial Agents and Chemotherapy 45, № 6 (2001): 1737–42. http://dx.doi.org/10.1128/aac.45.6.1737-1742.2001.
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ABSTRACT Compared to MICs (more than 800 μg/ml) of (−)-epigallocatechin gallate (EGCg) against Escherchia coli, MICs of EGCg against methicillin-susceptible and methicillin-resistantStaphylococcus aureus (MSSA and MRSA) were 100 μg/ml or less. Furthermore, less than 25 μg EGCg per ml obviously reversed the high level resistance of MRSA to all types of tested β-lactams, including benzylpenicillin, oxacillin, methicillin, ampicillin, and cephalexin. EGCg also induced a supersusceptibility to β-lactams in MSSA which does not express mecA, encoding penicillin-binding protein 2′ (PBP2′). The fractional inhibitory concentration (FIC) indices of the tested β-lactams against 25 isolates of MRSA were from 0.126 to 0.625 in combination with 6.25, 12.5 or 25 μg of EGCg per ml. However, no synergism was observed between EGCg and ampicillin against E. coli. EGCg largely reduced the tolerance of MRSA and MSSA to high ionic strength and low osmotic pressure in their external atmosphere, indicating damage of the cell wall. Unlike dextran and lipopolysaccharide, peptidoglycan fromS. aureus blocked both the antibacterial activity of EGCg and the synergism between EGCg and oxacillin, suggesting a direct binding of EGCg with peptidoglycan on the cell wall. EGCg showed a synergistic effect with dl-cycloserine (an inhibitor of cell wall synthesis unrelated to PBP2′) but additive or indifferent effect with inhibitors of protein and nuclear acid synthesis. EGCg did not suppress either PBP2′ mRNA expression or PBP2′ production, as confirmed by reverse transcription-PCR and a semiquantitative PBP2′ latex agglutination assay, indicating an irrelevance between the synergy and PBP2′ production. In summary, both EGCg and β-lactams directly or indirectly attack the same site, peptidoglycan on the cell wall. EGCg synergizes the activity of β-lactams against MRSA owing to interference with the integrity of the cell wall through direct binding to peptidoglycan.
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Vu, Hoang Anh, Yuuichi Beppu, Hoang Thanh Chi, et al. "Green Tea Epigallocatechin Gallate Exhibits Anticancer Effect in Human Pancreatic Carcinoma Cells via the Inhibition of Both Focal Adhesion Kinase and Insulin-Like Growth Factor-I Receptor." Journal of Biomedicine and Biotechnology 2010 (2010): 1–8. http://dx.doi.org/10.1155/2010/290516.
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The exact molecular mechanism by which epigallocatechin gallate (EGCG) suppresses human pancreatic cancer cell proliferation is unclear. We show here that EGCG-treated pancreatic cancer cells AsPC-1 and BxPC-3 decrease cell adhesion ability on micro-pattern dots, accompanied by dephosphorylations of both focal adhesion kinase (FAK) and insulin-like growth factor-1 receptor (IGF-1R) whereas retained the activations of mitogen-activated protein kinase and mammalian target of rapamycin. The growth of AsPC-1 and BxPC-3 cells can be significantly suppressed by EGCG treatment alone in a dose-dependent manner. At a dose of 100 μM which completely abolishes activations of FAK and IGF-1R, EGCG suppresses more than 50% of cell proliferation without evidence of apoptosis analyzed by PARP cleavage. Finally, the MEK1/2 inhibitor U0126 enhances growth-suppressive effect of EGCG. Our data suggests that blocking FAK and IGF-1R by EGCG could prove valuable for targeted therapy, which can be used in combination with other therapies, for pancreatic cancer.
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Ismiyatin, Kun, Devi Eka Juniarti, Djoko Agus Purwanto, and Adecha Ekalipta Primazafira. "Effect Of Epigallocatechin-3-Gallate (EGCG) On The Number Of Macrophage Cells In Inflammation Of Pulp With Mechanical Injury." Conservative Dentistry Journal 10, no. 1 (2020): 31. http://dx.doi.org/10.20473/cdj.v10i1.2020.31-35.
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Background: Pulpitis is an inflammatory pulp that can occur due to mechanical trauma that causes perforation of the pulp. Treatment of pulpitis Emergency frequently using Eugenol. High concentrations of Eugenol have a cytotoxic effect that causes local necrosis and inhibits the recovery process. An alternative consideration with Epigallocatechin 3-gallate (EGCG) which has good antioxidant properties and increases the complexity of inflammation by inhibiting the production of inducible nitric oxide synthase (iNOS) and nitric oxide so that levels migration of inflammatory cells to the area of injury will decrease and inflammation will occur shorter then initiates the proliferation so the recovery process and tissue repair will be more rapidly occurring. One of the cells that support tissue repair is macrophages. Purpose: this study aims to explain the effect of EGCG on the number of macrophage cells in pulp inflammation with mechanical injury. Methods: The study used 24 Wistar rats teeth divided into four groups, namely control (C), and 3 treatment groups T1, T2, and T3. Each group consisted of 6 rats prepared, then T1 was named EGCG hydrogel 60 ppm, T2 was given EGCG hydrogel 90 ppm, while T3 was given EGCG hydrogel 120 ppm. On the 3rd day, Wistar rats were decapitated to continue making HPA preparations. Results:This study showed a significant difference in each group (p< 0.05) using One-Way Anova analysis. Conclusion: EGCG hydrogel 90 ppm is effectivein increasing the number of macrophage cells.
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Pereyra-Bonnet, F., R. Bevacqua, A. Gibbons, et al. "430 INJECTION OF CELLS OR THEIR PARTS AFTER A SHORT EXPOSURE TO PLASMID CONSTRUCTS INDUCES TRANSGENESIS IN OVINE AND BOVINE EMBRYOS." Reproduction, Fertility and Development 22, no. 1 (2010): 372. http://dx.doi.org/10.1071/rdv22n1ab430.
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As animal transgenesis is an essential tool in medicine and agriculture, it is necessary to understand the mechanisms in order to develop novel methods of transgenesis. We intended to determine if the injection of cells or their parts into metaphase II (MII) oocytes after incubating with exogenous DNA can induce transgenesis in embryos. Sperm cells for intracytoplasmic sperm injection (ICSI) in ovine and cumulus cells for NT in bovine were incubated with pCX-EGFP plasmid (5 to 50 ng μL-1) for 5 min in 2.8% Na citrate at 0°C before transfer into a 10% polyvinylpyrrolidone (PVP) droplet and injection into MII oocytes (previously enucleated in NT). In both species, oolemma-ooplasmic vesicles (OOV) of 9 μm diameter obtained from MII oocytes by microsurgery were directly incubated in PVP droplet with same pCX-EGFP concentration. As a control group, pCX-EGFP suspension from PVP droplet was injected into MII oocytes. The NT bovine zygotes were activated in 5 μM ionomycin (Io) for 4 min followed by 1.9 mM DMAP immediately for 3 h. In ICSI ovine, the treatment with DMAP was applied 3 h later. Injected oocytes of OOV and controls were activated as NT in bovine and as ICSI in ovine. Expression of EGFP was determined with fluorescence microscopy under blue light (488 nm) at Days 4 to 7, and data were analyzed by Fisher test (P = 0.05). A group of NT, ICSI, OOV, and control presumptive zygotes were treated with FITC-labeled bovine fragments (100-300 bb) DNA in order to determine the binding sites with exogenous DNA by laser confocal microscope analysis. Quantitative PCR (qPCR) was performed to determine pCX-EGFP copy number at 0, 8, 16, and 24 h after Io in all ovine treatments. Embryos expressing EGDP from all techniques were subjected to FISH with rhodamine-labeled pCX-EGFP plasmid as a probe. In ovine, ICSI and OOV injection green embryos at Day 4 [58% (61/105) v. 21.5% (8/38); P < 0.05] and green blastocysts at Day 7 [71.8% (23/32) v. 66.6% (2/3)] were obtained, respectively. In bovine, green embryos [49.2% (34/69) v. 29.7% (14/47); P < 0.05] and green blastocysts [95.8% (23/24) v. 25.0% (2/8); P < 0.05] were produced by NT and OOV injection, respectively. In controls, no green embryos were obtained in ovine (0/47) and only low rates were observed in bovine [3.0% (2/65)]. Confocal images of zygotes showed specific signal only in cumulus cells, spermatozoa, and OOV The qPCR analysis showed similar plasmid copy number/zygote between treatment and times in ovine (range 30 000-300,000). Embryo FISH images showed 1 to 2 specific signals in ICSI and NT interphases of both species and in OOV ovine metaphases, the latter being direct evidence of transgene integration. These results suggest that the injected cells or cellular parts (OOV) dramatically increase transgenesis in ovine and bovine embryos. Until now, the generation of NT and OOV embryos after short exposure to the DNA construction has not been reported. We are performing embryo transfer and at the moment we have a pregnancy derived from ICSI in ewes. In conclusion, the cellular parts/transgene complex may affect exogenous DNA delivery or its interaction with embryo DNA, facilitating the mechanism of transgenesis in mammals.
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Nishida, Yuichiro, Kumpei Tokuyama, Shoichiro Nagasaka, et al. "SG, SI, and EGP of exercise-trained middle-aged men estimated by a two-compartment labeled minimal model." American Journal of Physiology-Endocrinology and Metabolism 283, no. 4 (2002): E809—E816. http://dx.doi.org/10.1152/ajpendo.00237.2001.
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To examine the effects of physical training on glucose effectiveness (SG), insulin sensitivity (SI), and endogenous glucose production (EGP) in middle-aged men, stable-labeled frequently sampled intravenous glucose tolerance tests (FSIGTT) were performed on 11 exercise-trained middle-aged men and 12 age-matched sedentary men. The time course of EGP during the FSIGTT was estimated by nonparametric stochastic deconvolution. Glucose uptake-specific indexes of glucose effectiveness (S[Formula: see text]*× 102: 0.81 ± 0.08 vs. 0.60 ± 0.05 dl · min−1· kg−1, P < 0.05) and insulin sensitivity [S[Formula: see text]* × 104: 24.59 ± 2.98 vs. 11.89 ± 2.36 dl · min−1· (μU/ml)−1· kg−1, P < 0.01], which were analyzed using the two-compartment minimal model, were significantly greater in the trained group than in the sedentary group. Plasma clearance rate (PCR) of glucose was consistently greater in the trained men than in sedentary men throughout FSIGTT. Compared with sedentary controls, EGP of trained middle-aged men was higher before glucose load. The EGP of the two groups was similarly suppressed by ∼70% within 10 min, followed by an additional suppression after insulin infusion. EGP returned to basal level at ∼60 min in the trained men and at 100 min in the controls, followed by its overshoot, which was significantly greater in the trained men than in the controls. In addition, basal EGP was positively correlated with S[Formula: see text]*. The higher basal EGP and greater EGP overshoot in trained middle-aged men appear to compensate for the increased insulin-independent (S[Formula: see text]*) and -dependent (S[Formula: see text]*) glucose uptake to maintain glucose homeostasis.
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Ahn, Hee-Yul, Kourosch Reza Hadizadeh, Claudia Seul, Yeo-Pyo Yun, Hans Vetter, and Agapios Sachinidis. "Epigallocathechin-3 Gallate Selectively Inhibits the PDGF-BB–induced Intracellular Signaling Transduction Pathway in Vascular Smooth Muscle Cells and Inhibits Transformation ofsis-transfected NIH 3T3 Fibroblasts and Human Glioblastoma Cells (A172)." Molecular Biology of the Cell 10, no. 4 (1999): 1093–104. http://dx.doi.org/10.1091/mbc.10.4.1093.
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Enhanced activity of receptor tyrosine kinases such as the PDGF β-receptor and EGF receptor has been implicated as a contributing factor in the development of malignant and nonmalignant proliferative diseases such as cancer and atherosclerosis. Several epidemiological studies suggest that green tea may prevent the development of cancer and atherosclerosis. One of the major constituents of green tea is the polyphenol epigallocathechin-3 gallate (EGCG). In an attempt to offer a possible explanation for the anti-cancer and anti-atherosclerotic activity of EGCG, we examined the effect of EGCG on the PDGF-BB–, EGF-, angiotensin II-, and FCS-induced activation of the 44 kDa and 42 kDa mitogen-activated protein (MAP) kinase isoforms (p44mapk/p42mapk) in cultured vascular smooth muscle cells (VSMCs) from rat aorta. VSMCs were treated with EGCG (1–100 μM) for 24 h and stimulated with the above mentioned agonists for different time periods. Stimulation of the p44mapk/p42mapk was detected by the enhanced Western blotting method using phospho-specific MAP kinase antibodies that recognized the Tyr204-phosphorylated (active) isoforms. Treatment of VSMCs with 10 and 50 μM EGCG resulted in an 80% and a complete inhibition of the PDGF-BB–induced activation of MAP kinase isoforms, respectively. In striking contrast, EGCG (1–100 μM) did not influence MAP kinase activation by EGF, angiotensin II, and FCS. Similarly, the maximal effect of PDGF-BB on the c-fos and egr-1 mRNA expression as well as on intracellular free Ca2+concentration was completely inhibited in EGCG-treated VSMCs, whereas the effect of EGF was not affected. Quantification of the immunoprecipitated tyrosine-phosphorylated PDGF-Rβ, phosphatidylinositol 3′-kinase, and phospholipase C-γ1 by the enhanced Western blotting method revealed that EGCG treatment effectively inhibits tyrosine phosphorylation of these kinases in VSMCs. Furthermore, we show that spheroid formation of human glioblastoma cells (A172) and colony formation ofsis-transfected NIH 3T3 cells in semisolid agar are completely inhibited by 20–50 μM EGCG. Our findings demonstrate that EGCG is a selective inhibitor of the tyrosine phosphorylation of PDGF-Rβ and its downstream signaling pathway. The present findings may partly explain the anti-cancer and anti-atherosclerotic activity of green tea.
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Dickson, Ameliya, Elise Cooper, Lenu B. Fakae, Bo Wang, Ka Lung Andrew Chan, and Hany M. Elsheikha. "In Vitro Growth- and Encystation-Inhibitory Efficacies of Matcha Green Tea and Epigallocatechin Gallate Against Acanthameoba Castellanii." Pathogens 9, no. 9 (2020): 763. http://dx.doi.org/10.3390/pathogens9090763.
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We examined the inhibitory effect of matcha green tea (Camellia sinensis) and epigallocatechin gallate (EGCg; the most abundant catechin in tea) on the vegetative growth and encystation of Acanthamoeba castellanii T4 genotype. The sulforhodamine B (SRB) stain-based colorimetric assay and hemocytometer counting were used to determine the reduction in A. castellanii trophozoite proliferation and encystation, in response to treatment with C. sinensis or EGCg. Fourier transform infrared (FTIR) microscopy was used to analyze chemical changes in the trophozoites and cysts due to C. sinensis treatment. Hot brewed and cold brewed matcha inhibited the growth of trophozoites by >40% at a 100 % concentration. EGCg at concentrations of 50 to 500 µM significantly inhibited the trophozoite growth compared to control. Hot brewed matcha (100% concentration) also showed an 87% reduction in the rate of encystation compared to untreated control. Although 500 µM of EGCg increased the rate of encystation by 36.3%, 1000 µM reduced it by 27.7%. Both percentages were not significant compared to control. C. sinensis induced more cytotoxicity to Madin Darby canine kidney cells compared to EGCg. FTIR chemical fingerprinting analysis showed that treatment with brewed matcha significantly increased the levels of glycogen and carbohydrate in trophozoites and cysts.
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Potenza, Maria A., Flora L. Marasciulo, Mariela Tarquinio, et al. "EGCG, a green tea polyphenol, improves endothelial function and insulin sensitivity, reduces blood pressure, and protects against myocardial I/R injury in SHR." American Journal of Physiology-Endocrinology and Metabolism 292, no. 5 (2007): E1378—E1387. http://dx.doi.org/10.1152/ajpendo.00698.2006.
Full textAbstract:
Epigallocatechin gallate (EGCG), a bioactive polyphenol in green tea, may augment metabolic and vascular actions of insulin. Therefore, we investigated effects of EGCG treatment to simultaneously improve cardiovascular and metabolic function in spontaneously hypertensive rats (SHR; model of metabolic syndrome with hypertension, insulin resistance, and overweight). In acute studies, EGCG (1–100 μM) elicited dose-dependent vasodilation in mesenteric vascular beds (MVB) isolated from SHR ex vivo that was inhibitable by Nω-nitro-l-arginine methyl ester (l-NAME; nitric oxide synthase antagonist) or wortmannin [phosphatidylinositol (PI) 3-kinase inhibitor]. In chronic studies, 9-wk-old SHR were treated by gavage for 3 wk with EGCG (200 mg·kg−1·day−1), enalapril (30 mg·kg−1·day−1), or vehicle. A separate group of SHR receiving l-NAME (80 mg/l in drinking water) was treated for 3 wk with either EGCG or vehicle. Vasodilator actions of insulin were significantly improved in MVB from EGCG- or enalapril-treated SHR (when compared with vehicle-treated SHR). Both EGCG and enalapril therapy significantly lowered systolic blood pressure (SBP) in SHR. EGCG therapy of SHR significantly reduced infarct size and improved cardiac function in Langendorff-perfused hearts exposed to ischemia-reperfusion (I/R) injury. In SHR given l-NAME, beneficial effects of EGCG on SBP and I/R were not observed. Both enalapril and EGCG treatment of SHR improved insulin sensitivity and raised plasma adiponectin levels. We conclude that acute actions of EGCG to stimulate production of nitric oxide from endothelium using PI 3-kinase-dependent pathways may explain, in part, beneficial effects of EGCG therapy to simultaneously improve metabolic and cardiovascular pathophysiology in SHR. These findings may be relevant to understanding potential benefits of green tea consumption in patients with the metabolic syndrome.
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Ismiyatin, Kun, Latief Mooduto, and Putri Dea Amani Faadhilah. "Effect of Epigallocatechin-3-Gallate (EGCG) on The Number of Lymphocyte Cells in Inflammation of Pulp with Mechanical Injury." Conservative Dentistry Journal 10, no. 1 (2020): 9. http://dx.doi.org/10.20473/cdj.v10i1.2020.9-13.
Full textAbstract:
Background: Pulpitis is an inflammatory pulp that can caused by pulp perforation by mechanical injury. Emergency treatment of pulpitis is using Eugenol. High concentrations of Eugenol have a cytotoxic effect that causes local necrosis and inhibits the healing process. Because of negative effects from eugenol, then it’s necessary to consider a new ingredient with minimal side effects, and it’s epigallocatechin-3-gallate (EGCG) in green tea. As a polyphenol, it has good antioxidant effect and plays a role in shortening the duration of inflammation by radical scavenging against Nitric Oxide so that NO levels rapidly decrease which causes migration of neutrophil cells to the area of injury will decrease and the inflammatory process faster so that the healing process become faster. Lymphocyte is plays a role in tissue repair. Purpose: to explain the effect of EGCG hydrogel on the number of lymphocyte cells in pulp inflammation with mechanical injury. Methods: The study used 24 Wistar rats divided into four groups, namely control (C), and 3 groups of treatment (T1, T2, T3). Each group consisted of 6 rats prepared, then EGCG hydrogel 60 ppm was named T1, EGCG hydrogel 90 ppm was named T2, EGCG hydrogel 120 ppm was named P3. On the 3rd day, Wistar rats were decapitated for HPA preparations. Results: This study showed a significant difference in each group (p < 0.05) using One-Way Anova analysis. Conclusion: EGCG hydrogel 90 ppm is effective in increasing the number of lymphocyte cells in inflammation of the pulp with mechanical injury.