Log in

Relevant bibliographies by topics / Ehrlich pathway / Journal articles

To see the other types of publications on this topic, follow the link: Ehrlich pathway.

Journal articles on the topic 'Ehrlich pathway'

Author: Grafiati

Published: 4 June 2021

Last updated: 9 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Ehrlich pathway.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Ivanov, Samuil Lyubomirov, Ventsislava Yankova Petrova, Emiliya Ivanova Pisareva, and Anna Vengelova Kujumdzieva. "Ehrlich Pathway Study inSaccharomycesandKluyveromycesYeasts." Biotechnology & Biotechnological Equipment 27, no. 5 (2013): 4103–10. http://dx.doi.org/10.5504/bbeq.2013.0078.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Lambert, Ian Henry. "Activation and inactivation of the volume-sensitive taurine leak pathway in NIH3T3 fibroblasts and Ehrlich Lettre ascites cells." American Journal of Physiology-Cell Physiology 293, no. 1 (2007): C390—C400. http://dx.doi.org/10.1152/ajpcell.00104.2007.

Full text

Abstract:

Hypotonic exposure provokes the mobilization of arachidonic acid, production of ROS, and a transient increase in taurine release in Ehrlich Lettre cells. The taurine release is potentiated by H2O2 and the tyrosine phosphatase inhibitor vanadate and reduced by the phospholipase A2 (PLA2) inhibitors bromoenol lactone (BEL) and manoalide, the 5-lipoxygenase (5-LO) inhibitor ETH-615139, the NADPH oxidase inhibitor diphenyl iodonium (DPI), and antioxidants. Thus, swelling-induced taurine efflux in Ehrlich Lettre cells involves Ca2+-independent (iPLA2)/secretory PLA2 (sPLA2) plus 5-LO activity and m

APA, Harvard, Vancouver, ISO, and other styles

3

Brunke, Sascha, Katja Seider, Ricardo Sergio Almeida, et al. "Candida glabratatryptophan-based pigment production via the Ehrlich pathway." Molecular Microbiology 76, no. 1 (2010): 25–47. http://dx.doi.org/10.1111/j.1365-2958.2010.07052.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Pimenova, E. A., Yu A. Reunova, E. S. Menchinskaya, A. A. Reunov, and D. L. Aminin. "An Unusual Pathway of Mitoptosis Found in Ehrlich Carcinoma Cells." Doklady Biological Sciences 494, no. 1 (2020): 240–43. http://dx.doi.org/10.1134/s0012496620050063.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Vuralhan, Zeynep, Marcos A. Morais, Siew-Leng Tai, Matthew D. W. Piper, and Jack T. Pronk. "Identification and Characterization of Phenylpyruvate Decarboxylase Genes in Saccharomyces cerevisiae." Applied and Environmental Microbiology 69, no. 8 (2003): 4534–41. http://dx.doi.org/10.1128/aem.69.8.4534-4541.2003.

Full text

Abstract:

ABSTRACT Catabolism of amino acids via the Ehrlich pathway involves transamination to the corresponding α-keto acids, followed by decarboxylation to an aldehyde and then reduction to an alcohol. Alternatively, the aldehyde may be oxidized to an acid. This pathway is functional in Saccharomyces cerevisiae, since during growth in glucose-limited chemostat cultures with phenylalanine as the sole nitrogen source, phenylethanol and phenylacetate were produced in quantities that accounted for all of the phenylalanine consumed. Our objective was to identify the structural gene(s) required for the dec

APA, Harvard, Vancouver, ISO, and other styles

6

El-Ashmawy, Nahla E., Naglaa F. Khedr, Hoda A. El-Bahrawy, and Hend E. Abo Mansour. "Metformin augments doxorubicin cytotoxicity in mammary carcinoma through activation of adenosine monophosphate protein kinase pathway." Tumor Biology 39, no. 5 (2017): 101042831769223. http://dx.doi.org/10.1177/1010428317692235.

Full text

Abstract:

Since the incidence of breast cancer increases dramatically all over the world, the search for effective treatment is an urgent need. Metformin has demonstrated anti-tumorigenic effect both in vivo and in vitro in different cancer types. This work was designed to examine on molecular level the mode of action of metformin in mice bearing solid Ehrlich carcinoma and to evaluate the use of metformin in conjunction with doxorubicin as a combined therapy against solid Ehrlich carcinoma. Ehrlich ascites carcinoma cells were inoculated in 60 female mice as a model of breast cancer. The mice were divi

APA, Harvard, Vancouver, ISO, and other styles

7

Calixto-Campos, Cassia, Mab P. Corrêa, Thacyana T. Carvalho, et al. "Quercetin Reduces Ehrlich Tumor-Induced Cancer Pain in Mice." Analytical Cellular Pathology 2015 (2015): 1–18. http://dx.doi.org/10.1155/2015/285708.

Full text

Abstract:

Cancer pain directly affects the patient’s quality of life. We have previously demonstrated that the subcutaneous administration of the mammary adenocarcinoma known as Ehrlich tumor induces pain in mice. Several studies have shown that the flavonoid quercetin presents important biological effects, including anti-inflammatory, antioxidant, analgesic, and antitumor activity. Therefore, the analgesic effect and mechanisms of quercetin were evaluated in Ehrlich tumor-induced cancer pain in mice. Intraperitoneal (i.p.) treatments with quercetin reduced Ehrlich tumor-induced mechanical and thermal h

APA, Harvard, Vancouver, ISO, and other styles

8

Ourique, Fabiana, Maicon R. Kviecinski, Karina B. Felipe, et al. "DNA Damage and Inhibition of Akt Pathway in MCF-7 Cells and Ehrlich Tumor in Mice Treated with 1,4-Naphthoquinones in Combination with Ascorbate." Oxidative Medicine and Cellular Longevity 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/495305.

Full text

Abstract:

The aim of this study was to enhance the understanding of the antitumor mechanism of 1,4-naphthoquinones and ascorbate. Juglone, phenylaminonaphthoquinone-7, and 9 (Q7/Q9) were evaluated for effects on CT-DNA and DNA of cancer cells. Evaluations in MCF-7 cells are DNA damage, ROS levels, viability, and proliferation. Proteins from MCF-7 lysates were immunoblotted for verifying PARP integrity,γH2AX, and pAkt. Antitumor activity was measured in Ehrlich ascites carcinoma-bearing mice. The same markers of molecular toxicity were assessedin vivo. The naphthoquinones intercalate into CT-DNA and caus

APA, Harvard, Vancouver, ISO, and other styles

9

Gu, Yang, Jingbo Ma, Yonglian Zhu, and Peng Xu. "Refactoring Ehrlich Pathway for High-Yield 2-Phenylethanol Production in Yarrowia lipolytica." ACS Synthetic Biology 9, no. 3 (2020): 623–33. http://dx.doi.org/10.1021/acssynbio.9b00468.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Kim, Bosu, Bo-Ram Cho, and Ji-Sook Hahn. "Metabolic engineering ofSaccharomyces cerevisiaefor the production of 2-phenylethanol via Ehrlich pathway." Biotechnology and Bioengineering 111, no. 1 (2013): 115–24. http://dx.doi.org/10.1002/bit.24993.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Yin, Sheng, Hui Zhou, Xiao Xiao, Tiandan Lang, Jingru Liang, and Chengtao Wang. "Improving 2-Phenylethanol Production via Ehrlich Pathway Using Genetic Engineered Saccharomyces cerevisiae Strains." Current Microbiology 70, no. 5 (2015): 762–67. http://dx.doi.org/10.1007/s00284-015-0785-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Li, Shanshan, Jianping Wen, and Xiaoqiang Jia. "Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression." Applied Microbiology and Biotechnology 91, no. 3 (2011): 577–89. http://dx.doi.org/10.1007/s00253-011-3280-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Uzunov, Zlatyo Georgiev, Ventsislava Yankova Petrova, Samuil Lyubomirov Ivanov, and Anna Vangelova Kujumdzieva. "In SilicoStudy of Aro Genes Involved in the Ehrlich Pathway: Comparison betweenSaccharomyces CerevisiaeandKluyveromyces Lactis." Biotechnology & Biotechnological Equipment 25, sup1 (2011): 133–37. http://dx.doi.org/10.5504/bbeq.2011.0128.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Ravasio, Davide, Jürgen Wendland, and Andrea Walther. "Major contribution of the Ehrlich pathway for 2-phenylethanol/rose flavor production inAshbya gossypii." FEMS Yeast Research 14, no. 6 (2014): 833–44. http://dx.doi.org/10.1111/1567-1364.12172.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Hazelwood, Lucie A., Jean-Marc Daran, Antonius J. A. van Maris, Jack T. Pronk, and J. Richard Dickinson. "The Ehrlich Pathway for Fusel Alcohol Production: a Century of Research on Saccharomyces cerevisiae Metabolism." Applied and Environmental Microbiology 74, no. 8 (2008): 2259–66. http://dx.doi.org/10.1128/aem.02625-07.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Hazelwood, Lucie A., Jean-Marc Daran, Antonius J. A. van Maris, Jack T. Pronk, and J. Richard Dickinson. "The Ehrlich Pathway for Fusel Alcohol Production: a Century of Research on Saccharomyces cerevisiae Metabolism." Applied and Environmental Microbiology 74, no. 12 (2008): 3920. http://dx.doi.org/10.1128/aem.00934-08.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Hwang, Joon-Young, Jihyang Park, Joo-Hyun Seo, et al. "Simultaneous synthesis of 2-phenylethanol and L-homophenylalanine using aromatic transaminase with yeast Ehrlich pathway." Biotechnology and Bioengineering 102, no. 5 (2009): 1323–29. http://dx.doi.org/10.1002/bit.22178.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Nabeel, Asmaa I. "Samarium enriches antitumor activity of ZnO nanoparticles via downregulation of CXCR4 receptor and cytochrome P450." Tumor Biology 42, no. 3 (2020): 101042832090999. http://dx.doi.org/10.1177/1010428320909999.

Full text

Abstract:

Cancer is the leading cause of death and exhausts human and economic resources for treatment and protection. Zinc oxide nanoparticles play an effective role in tumor treatment but with some cautions, such as overexpression of cytochrome P450, hepatic overload, and the mammalian target of rapamycin pathway resistance. Although lanthanides have antitumor activity, their use is limited. Therefore, the current study aims to improve the effectiveness of zinc oxide nanoparticle via doping with lanthanides, such as samarium. In vitro study revealed that samarium doped with zinc oxide showed more anti

APA, Harvard, Vancouver, ISO, and other styles

19

Swarna, Rubaiya Rafique, A. K. M. Asaduzzaman, Syed Rashel Kabir, et al. "Antiproliferative and Antimicrobial Potentials of a Lectin from Aplysia kurodai (Sea Hare) Eggs." Marine Drugs 19, no. 7 (2021): 394. http://dx.doi.org/10.3390/md19070394.

Full text

Abstract:

In recent years, there has been considerable interest in lectins from marine invertebrates. In this study, the biological activities of a lectin protein isolated from the eggs of Sea hare (Aplysia kurodai) were evaluated. The 40 kDa Aplysia kurodai egg lectin (or AKL-40) binds to D-galacturonic acid and D-galactose sugars similar to previously purified isotypes with various molecular weights (32/30 and 16 kDa). The N-terminal sequence of AKL-40 was similar to other sea hare egg lectins. The lectin was shown to be moderately toxic to brine shrimp nauplii, with an LC50 value of 63.63 µg/mL. It a

APA, Harvard, Vancouver, ISO, and other styles

20

Shen, Li, Yuya Nishimura, Fumio Matsuda, Jun Ishii, and Akihiko Kondo. "Overexpressing enzymes of the Ehrlich pathway and deleting genes of the competing pathway in Saccharomyces cerevisiae for increasing 2-phenylethanol production from glucose." Journal of Bioscience and Bioengineering 122, no. 1 (2016): 34–39. http://dx.doi.org/10.1016/j.jbiosc.2015.12.022.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Scott, William T., Eddy J. Smid, Richard A. Notebaart, and David E. Block. "Curation and Analysis of a Saccharomyces cerevisiae Genome-Scale Metabolic Model for Predicting Production of Sensory Impact Molecules under Enological Conditions." Processes 8, no. 9 (2020): 1195. http://dx.doi.org/10.3390/pr8091195.

Full text

Abstract:

One approach for elucidating strain-to-strain metabolic differences is the use of genome-scale metabolic models (GSMMs). To date GSMMs have not focused on the industrially important area of flavor production and, as such; do not cover all the pathways relevant to flavor formation in yeast. Moreover, current models for Saccharomyces cerevisiae generally focus on carbon-limited and/or aerobic systems, which is not pertinent to enological conditions. Here, we curate a GSMM (iWS902) to expand on the existing Ehrlich pathway and ester formation pathways central to aroma formation in industrial wine

APA, Harvard, Vancouver, ISO, and other styles

22

Wang, Zhaoyue, Xuejing Bai, Xuena Guo, and Xiuping He. "Regulation of crucial enzymes and transcription factors on 2-phenylethanol biosynthesis via Ehrlich pathway in Saccharomyces cerevisiae." Journal of Industrial Microbiology & Biotechnology 44, no. 1 (2016): 129–39. http://dx.doi.org/10.1007/s10295-016-1852-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Saraswati, Sarita, S. S. Agrawal, and Abdulqader A. Alhaider. "Ursolic acid inhibits tumor angiogenesis and induces apoptosis through mitochondrial-dependent pathway in Ehrlich ascites carcinoma tumor." Chemico-Biological Interactions 206, no. 2 (2013): 153–65. http://dx.doi.org/10.1016/j.cbi.2013.09.004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Moustafa, Enas Mahmoud, Noura Magdy Thabet, and Khaled Shaaban Azab. "Boswellic acid disables signal transduction of IL-6–STAT-3 in Ehrlich ascites tumor bearing irradiated mice." Biochemistry and Cell Biology 94, no. 4 (2016): 307–13. http://dx.doi.org/10.1139/bcb-2015-0169.

Full text

Abstract:

Boswellic acid (BA) is known for its ability to trigger apoptosis as well as to inhibit angiogenesis in tumor tissue. In this study, we investigated the effect of BA on the IL-6–STAT-3 signalling pathway in irradiated mice bearing solid tumors of Ehrlich ascites carcinoma (EAC). For this, we administered BA (25 mg·(kg body mass)–1·day–1, by intraperitoneal injection) to mice with EAC, and then exposed them to 4 Gy of gamma radiation. Data analyses of the results revealed a specific impact from BA on IL-6R mRNA and survivin mRNA in EACs and irradiated EAC-bearing mice. Also, significant improve

APA, Harvard, Vancouver, ISO, and other styles

25

Hoffmann, E. K., M. Schiodt, and P. Dunham. "The number of chloride-cation cotransport sites on Ehrlich ascites cells measured with [3H]bumetanide." American Journal of Physiology-Cell Physiology 250, no. 5 (1986): C688—C693. http://dx.doi.org/10.1152/ajpcell.1986.250.5.c688.

Full text

Abstract:

Simultaneous measurements were made of net Cl influxes and [3H]bumetanide binding to Ehrlich ascites tumor cells in which the chloride-cation cotransport pathway had been activated by hypertonic challenge. There was a good linear correlation between inhibition of Cl influx during regulatory volume increase and numbers of bumetanide molecules bound per cell, consistent with high specificity of bumetanide binding to cotransport sites. The extrapolation to the number of bumetanide binding sites per cell at maximal inhibition of Cl transport thus gives the number of cotransport sites per cell as 2

APA, Harvard, Vancouver, ISO, and other styles

26

Black, William B., Edward King, Yixi Wang, et al. "Engineering a Coenzyme A Detour To Expand the Product Scope and Enhance the Selectivity of the Ehrlich Pathway." ACS Synthetic Biology 7, no. 12 (2018): 2758–64. http://dx.doi.org/10.1021/acssynbio.8b00358.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Ignacak, Jan, Maria Wiśniewska-Wrona, Joanna Dulinska-Litewka, Iwona Palka, and Magdalena Kucharska. "INHIBITION OF EHRLICH ASCITES TUMOUR (EAT) CELLS PROLIFERATION THROUGH CHITOSAN-MEDIATED REGULATION OF ACTIVITY OF THE AKT PATHWAY." Progress on Chemistry and application of Chitin and its Derivatives 19, no. 1 (2014): 33–40. http://dx.doi.org/10.15259/pcacd.19.04.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Hunt, Kristopher A., Natasha D. Mallette, Brent M. Peyton, and Ross P. Carlson. "In Silico Analysis of Functionalized Hydrocarbon Production Using Ehrlich Pathway and Fatty Acid Derivatives in an Endophytic Fungus." Journal of Fungi 7, no. 6 (2021): 435. http://dx.doi.org/10.3390/jof7060435.

Full text

Abstract:

Functionalized hydrocarbons have various ecological and industrial uses, from signaling molecules and antifungal/antibacterial agents to fuels and specialty chemicals. The potential to produce functionalized hydrocarbons using the cellulolytic, endophytic fungus, Ascocoryne sarcoides, was quantified using genome-enabled, stoichiometric modeling. In silico analysis identified available routes to produce these hydrocarbons, including both anabolic- and catabolic-associated strategies, and determined correlations between the type and size of the hydrocarbons and culturing conditions. The analysis

APA, Harvard, Vancouver, ISO, and other styles

29

Veiga, Tânia, Daniel Solis-Escalante, Gabriele Romagnoli, et al. "Resolving Phenylalanine Metabolism Sheds Light on Natural Synthesis of Penicillin G in Penicillium chrysogenum." Eukaryotic Cell 11, no. 2 (2011): 238–49. http://dx.doi.org/10.1128/ec.05285-11.

Full text

Abstract:

ABSTRACTThe industrial production of penicillin G byPenicillium chrysogenumrequires the supplementation of the growth medium with the side chain precursor phenylacetate. The growth ofP. chrysogenumwith phenylalanine as the sole nitrogen source resulted in the extracellular production of phenylacetate and penicillin G. To analyze this natural pathway for penicillin G production, chemostat cultures were switched to [U-13C]phenylalanine as the nitrogen source. The quantification and modeling of the dynamics of labeled metabolites indicated that phenylalanine was (i) incorporated in nascent protei

APA, Harvard, Vancouver, ISO, and other styles

30

Müller, Nikolaus. "Thiaminpyrophosphat – ein natürlich vorkommendes Iminium-Salz. Seine Bedeutung für die mikrobiellen Prozesse bei der Weinbereitung und die Aromabildung im Wein / Thiamine Diphosphate – A Natural Iminium Salt. Its Role in Microbiological Processes During Wine Preparation and on the Aroma of Wine." Zeitschrift für Naturforschung B 69, no. 5 (2014): 489–500. http://dx.doi.org/10.5560/znb.2014-4052.

Full text

Abstract:

Iminium salt structures are widespread in nature and play an important role in biochemical processes. Among these thiamine diphosphate (ThPP, Vitamine B1) is one of the best known examples. It serves as a cofactor in a variety of different enzymes that are found in all forms of life. During fermentation for the production of alcoholic beverages, ThPP is of great importance not only for the key step (decarboxylation of pyruvate), but also for the formation of secondary metabolites which highly influence the aroma of wine. Thus, enzymatic degradation of amino acids in yeasts via the Ehrlich path

APA, Harvard, Vancouver, ISO, and other styles

31

Kondo, Takashi, Hironori Tezuka, Jun Ishii, Fumio Matsuda, Chiaki Ogino, and Akihiko Kondo. "Genetic engineering to enhance the Ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae." Journal of Biotechnology 159, no. 1-2 (2012): 32–37. http://dx.doi.org/10.1016/j.jbiotec.2012.01.022.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Chen, Xianrui, Zhaoyue Wang, Xuena Guo, Sha Liu, and Xiuping He. "Regulation of general amino acid permeases Gap1p, GATA transcription factors Gln3p and Gat1p on 2-phenylethanol biosynthesis via Ehrlich pathway." Journal of Biotechnology 242 (January 2017): 83–91. http://dx.doi.org/10.1016/j.jbiotec.2016.11.028.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Kwiecień, Inga, Małgorzata Iciek та Lidia Włodek. "Acceleration of Anaerobic Cysteine Transformations to Sulfane Sulfur Consequent toγ-Glutamyl Transpeptidase Inhibition". Scientific World Journal 2012 (2012): 1–8. http://dx.doi.org/10.1100/2012/253724.

Full text

Abstract:

Toxicity of drugs and radiation in the cells is largely dependent on the level of thiols. In the present studies, an attempt has been made to inhibit γ-glutamyl transpeptidase (γGT) activity in EAT-bearing animals tissue. We have expected that administration of γGT inhibitors: acivicin and 1,2,3,4-tetrahydroisoquinoline (TIQ) may influence GSH/γ–glutamyl transpeptidase (γGT) system in the regulation of cysteine concentration and anaerobic cysteine metabolism in normal and cancer cells. Development of Ehrlich ascites tumor in mice enhances peroxidative processes, diminishes levels of nonprotein

APA, Harvard, Vancouver, ISO, and other styles

34

Toniato, Elena, Vincenzo Flati, Maria Grazia Cifone, et al. "Involvement of an Arachidonic-Acid-Dependent Pathway in the Interferon-beta-Mediated Expression of C202 Gene in Ehrlich-Ascites-Tumor Cells." European Journal of Biochemistry 235, no. 1-2 (1996): 91–96. http://dx.doi.org/10.1111/j.1432-1033.1996.00091.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Hasan, Md Mahmudul, Md Shihabul Islam, Kazi Md Faisal Hoque, Ariful Haque, and Md Abu Reza. "Effect of Citrus macroptera Fruit Pulp Juice on Alteration of Caspase Pathway Rendering Anti-Proliferative Activity against Ehrlichs Ascites Carcinoma in Mice." Toxicological Research 35, no. 3 (2019): 271–77. http://dx.doi.org/10.5487/tr.2019.35.3.271.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Su, Ying, Pauline Seguinot, Audrey Bloem, et al. "Isotopic Tracers Unveil Distinct Fates for Nitrogen Sources during Wine Fermentation with Two Non-Saccharomyces Strains." Microorganisms 8, no. 6 (2020): 904. http://dx.doi.org/10.3390/microorganisms8060904.

Full text

Abstract:

Non-Saccharomyces yeast strains have become increasingly prevalent in the food industry, particularly in winemaking, because of their properties of interest both in biological control and in complexifying flavour profiles in end-products. However, unleashing the full potential of these species would require solid knowledge of their physiology and metabolism, which is, however, very limited to date. In this study, a quantitative analysis using 15N-labelled NH4Cl, arginine, and glutamine, and 13C-labelled leucine and valine revealed the specificities of the nitrogen metabolism pattern of two non

APA, Harvard, Vancouver, ISO, and other styles

37

Varman, Arul M., Yi Xiao, Himadri B. Pakrasi, and Yinjie J. Tang. "Metabolic Engineering of Synechocystis sp. Strain PCC 6803 for Isobutanol Production." Applied and Environmental Microbiology 79, no. 3 (2012): 908–14. http://dx.doi.org/10.1128/aem.02827-12.

Full text

Abstract:

ABSTRACTGlobal warming and decreasing fossil fuel reserves have prompted great interest in the synthesis of advanced biofuels from renewable resources. In an effort to address these concerns, we performed metabolic engineering of the cyanobacteriumSynechocystissp. strain PCC 6803 to develop a strain that can synthesize isobutanol under both autotrophic and mixotrophic conditions. With the expression of two heterologous genes from the Ehrlich pathway, the engineered strain can accumulate 90 mg/liter of isobutanol from 50 mM bicarbonate in a gas-tight shaking flask. The strain does not require a

APA, Harvard, Vancouver, ISO, and other styles

38

Kovačević, Z., J. Popović, O. Brkljač, and S. Lelas. "Interaction of metabolism of aspartate and inosine and energy state of malignant cells." Biochemical Journal 247, no. 1 (1987): 47–51. http://dx.doi.org/10.1042/bj2470047.

Full text

Abstract:

1. Oxidation of glutamine in Ehrlich ascites-carcinoma cells results in a large accumulation of aspartate. 2. The addition of inosine causes a marked decrease in aspartate production from glutamine. This may be related to the resynthesis of AMP from aspartate and IMP, the latter being produced from inosine via the salvage pathway for purine nucleotides. In accordance with this assumption, a significant production of lactate was observed, which comes probably from the ribose moiety of inosine. Since lactate is known to inhibit production of aspartate from glutamine, this may explain the effect

APA, Harvard, Vancouver, ISO, and other styles

39

Zinchenko, Valeriy P., Nikolay V. Goncharov, Vera V. Teplova, et al. "Polarographic and spectroscopic studies of the effects of fluoroacetate/fluorocitrate on cells and mitochondria." Spectroscopy 21, no. 2 (2007): 121–34. http://dx.doi.org/10.1155/2007/717296.

Full text

Abstract:

Experiments were performed with rat liver mitochondria, Ehrlich ascite tumor cells (EATC) and cardiomyocytes, exposed to fluoroacetate (FA) or fluorocitrate (FC)in vitro. The effects of FA developed at much higher concentrations in comparison with FC and was dependent upon respiratory substrates: with pyruvate, FA induced a slow oxidation of pyridine nucleotides (NAD(P)H) and inhibition of respiration. NAD(P)H oxidation was prevented by incubation of mitochondria with cyclosporin A (CsA), an inhibitor of mitochondrial permeability transition pore. Studies of the NAD(P)H level and calcium respo

APA, Harvard, Vancouver, ISO, and other styles

40

Rasmussen, Maria, R. Todd Alexander, Barbara V. Darborg, et al. "Osmotic cell shrinkage activates ezrin/radixin/moesin (ERM) proteins: activation mechanisms and physiological implications." American Journal of Physiology-Cell Physiology 294, no. 1 (2008): C197—C212. http://dx.doi.org/10.1152/ajpcell.00268.2007.

Full text

Abstract:

Hyperosmotic shrinkage induces multiple cellular responses, including activation of volume-regulatory ion transport, cytoskeletal reorganization, and cell death. Here we investigated the possible roles of ezrin/radixin/moesin (ERM) proteins in these events. Osmotic shrinkage of Ehrlich Lettre ascites cells elicited the formation of long microvillus-like protrusions, rapid translocation of endogenous ERM proteins and green fluorescent protein-tagged ezrin to the cortical region including these protrusions, and Thr567/564/558 (ezrin/radixin/moesin) phosphorylation of cortical ERM proteins. Reduc

APA, Harvard, Vancouver, ISO, and other styles

41

Nirgude, Snehal, Raghunandan Mahadeva, Jinsha Koroth, et al. "ST09, A Novel Curcumin Derivative, Blocks Cell Migration by Inhibiting Matrix Metalloproteases in Breast Cancer Cells and Inhibits Tumor Progression in EAC Mouse Tumor Models." Molecules 25, no. 19 (2020): 4499. http://dx.doi.org/10.3390/molecules25194499.

Full text

Abstract:

Purpose: Curcumin is known for its anticancer and migrastatic activity in various cancers, including breast cancer. Newer curcumin derivatives are being explored to overcome limitations of curcumin like low bioavailability, stability, and side effects due to its higher dose. In this study, the synthesis of ST09, a novel curcumin derivative, and its antiproliferative, cytotoxic, and migrastatic properties have been explored both in vitro and in vivo. Methods: After ST09 synthesis, anticancer activity was studied by performing standard cytotoxicity assays namely, lactate dehydrogenase (LDH) rele

APA, Harvard, Vancouver, ISO, and other styles

42

Clement, T., M. Perez, J. R. Mouret, I. Sanchez, J. M. Sablayrolles, and C. Camarasa. "Metabolic Responses of Saccharomyces cerevisiae to Valine and Ammonium Pulses during Four-Stage Continuous Wine Fermentations." Applied and Environmental Microbiology 79, no. 8 (2013): 2749–58. http://dx.doi.org/10.1128/aem.02853-12.

Full text

Abstract:

ABSTRACTNitrogen supplementation, which is widely used in winemaking to improve fermentation kinetics, also affects the products of fermentation, including volatile compounds. However, the mechanisms underlying the metabolic response of yeast to nitrogen additions remain unclear. We studied the consequences forSaccharomyces cerevisiaemetabolism of valine and ammonium pulses during the stationary phase of four-stage continuous fermentation (FSCF). This culture technique provides cells at steady state similar to that of the stationary phase of batch wine fermentation. Thus, the FSCF device is an

APA, Harvard, Vancouver, ISO, and other styles

43

ter Schure, Eelko G., Marcel T. Flikweert, Johannes P. van Dijken, Jack T. Pronk, and C. Theo Verrips. "Pyruvate Decarboxylase Catalyzes Decarboxylation of Branched-Chain 2-Oxo Acids but Is Not Essential for Fusel Alcohol Production by Saccharomyces cerevisiae." Applied and Environmental Microbiology 64, no. 4 (1998): 1303–7. http://dx.doi.org/10.1128/aem.64.4.1303-1307.1998.

Full text

Abstract:

ABSTRACT The fusel alcohols 3-methyl-1-butanol, 2-methyl-1-butanol, and 2-methyl-propanol are important flavor compounds in yeast-derived food products and beverages. The formation of these compounds from branched-chain amino acids is generally assumed to occur via the Ehrlich pathway, which involves the concerted action of a branched-chain transaminase, a decarboxylase, and an alcohol dehydrogenase. Partially purified preparations of pyruvate decarboxylase (EC 4.1.1.1 ) have been reported to catalyze the decarboxylation of the branched-chain 2-oxo acids formed upon transamination of leucine,

APA, Harvard, Vancouver, ISO, and other styles

44

Vuralhan, Zeynep, Marijke A. H. Luttik, Siew Leng Tai, et al. "Physiological Characterization of the ARO10-Dependent, Broad-Substrate-Specificity 2-Oxo Acid Decarboxylase Activity of Saccharomyces cerevisiae." Applied and Environmental Microbiology 71, no. 6 (2005): 3276–84. http://dx.doi.org/10.1128/aem.71.6.3276-3284.2005.

Full text

Abstract:

ABSTRACT Aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae CEN.PK113-7D were grown with different nitrogen sources. Cultures grown with phenylalanine, leucine, or methionine as a nitrogen source contained high levels of the corresponding fusel alcohols and organic acids, indicating activity of the Ehrlich pathway. Also, fusel alcohols derived from the other two amino acids were detected in the supernatant, suggesting the involvement of a common enzyme activity. Transcript level analysis revealed that among the five thiamine-pyrophospate-dependent decarboxylases (PDC1, PDC

APA, Harvard, Vancouver, ISO, and other styles

45

Romagnoli, Gabriele, Marijke A. H. Luttik, Peter Kötter, Jack T. Pronk, and Jean-Marc Daran. "Substrate Specificity of Thiamine Pyrophosphate-Dependent 2-Oxo-Acid Decarboxylases in Saccharomyces cerevisiae." Applied and Environmental Microbiology 78, no. 21 (2012): 7538–48. http://dx.doi.org/10.1128/aem.01675-12.

Full text

Abstract:

ABSTRACTFusel alcohols are precursors and contributors to flavor and aroma compounds in fermented beverages, and some are under investigation as biofuels. The decarboxylation of 2-oxo acids is a key step in the Ehrlich pathway for fusel alcohol production. InSaccharomyces cerevisiae, five genes share sequence similarity with genes encoding thiamine pyrophosphate-dependent 2-oxo-acid decarboxylases (2ODCs).PDC1,PDC5, andPDC6encode differentially regulated pyruvate decarboxylase isoenzymes;ARO10encodes a 2-oxo-acid decarboxylase with broad substrate specificity, andTHI3has not yet been shown to

APA, Harvard, Vancouver, ISO, and other styles

46

Derrick, S., and P. J. Large. "Activities of the enzymes of the Ehrlich pathway and formation of branched-chain alcohols in Saccharomyces cerevisiae and Candida utilis grown in continuous culture on valine or ammonium as sole nitrogen source." Journal of General Microbiology 139, no. 11 (1993): 2783–92. http://dx.doi.org/10.1099/00221287-139-11-2783.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Demir, G., H. O. Klein, S. Yildirim, I. Esen, and T. Altug. "Gemcitabine and capecitabine synergism and biomodulation with dipridamole in a mouse tumor model." Journal of Clinical Oncology 24, no. 18_suppl (2006): 12034. http://dx.doi.org/10.1200/jco.2006.24.18_suppl.12034.

Full text

Abstract:

12034 Background and Aim: Gemcitabine and capecitabine are antimetabolites that are effective in the pyrimidine pathway of the DNA synthesis. Capecitabine through a 3-step activation process is transformed to 5-FU in the tumor cell by thymidine phosphorilase. On the other hand, gemcitabine interacts with the enzymes thymidine kinase, tymidine phosphorilase ve ribonucloetide reductase. Our hypothesis is based on a possible synergistic blockade by concurrent administration of these two antimetabolites. Additionally, dipridamole is an inhibitor of the nucleoside transport; and that can prolong th

APA, Harvard, Vancouver, ISO, and other styles

48

Kirkegaard, Signe Skyum, Ian Henry Lambert, Steen Gammeltoft, and Else Kay Hoffmann. "Activation of the TASK-2 channel after cell swelling is dependent on tyrosine phosphorylation." American Journal of Physiology-Cell Physiology 299, no. 4 (2010): C844—C853. http://dx.doi.org/10.1152/ajpcell.00024.2010.

Full text

Abstract:

The swelling-activated K+ currents ( IK,vol) in Ehrlich ascites tumor cells (EATC) has been reported to be through the two-pore domain (K2p), TWIK-related acid-sensitive K+ channel 2 (TASK-2). The regulatory volume decrease (RVD), following hypotonic exposure in EATC, is rate limited by IK,vol indicating that inhibition of RVD reflects inhibition of TASK-2. We find that in EATC the tyrosine kinase inhibitor genistein inhibits RVD by 90%, and that the tyrosine phosphatase inhibitor monoperoxo(picolinato)-oxo-vanadate(V) [mpV(pic)] shifted the volume set point for inactivation of the channel to

APA, Harvard, Vancouver, ISO, and other styles

49

Luo, Tian, Paige S. Dunphy, Taslima T. Lina, and Jere W. McBride. "Ehrlichia chaffeensis Exploits Canonical and Noncanonical Host Wnt Signaling Pathways To Stimulate Phagocytosis and Promote Intracellular Survival." Infection and Immunity 84, no. 3 (2015): 686–700. http://dx.doi.org/10.1128/iai.01289-15.

Full text

Abstract:

Ehrlichia chaffeensisinvades and survives in phagocytes by modulating host cell processes and evading innate defenses, but the mechanisms are not fully defined. Recently we have determined thatE. chaffeensistandem repeat proteins (TRPs) are type 1 secreted effectors involved in functionally diverse interactions with host targets, including components of the evolutionarily conserved Wnt signaling pathways. In this study, we demonstrated that induction of host canonical and noncanonical Wnt pathways byE. chaffeensisTRP effectors stimulates phagocytosis and promotes intracellular survival. AfterE

APA, Harvard, Vancouver, ISO, and other styles

50

Montero, M., J. Alvarez, and J. Garcia-Sancho. "Uptake of Ca2+ and refilling of intracellular Ca2+ stores in Ehrlich-ascites-tumour cells and in rat thymocytes." Biochemical Journal 271, no. 2 (1990): 535–40. http://dx.doi.org/10.1042/bj2710535.

Full text

Abstract:

We have studied the uptake of Ca2+ and its redistribution between the cytoplasm and the intracellular stores in Ehrlich-ascites-tumour cells and rat thymocytes previously depleted of Ca2+ by incubation in Ca2(+)-free medium. Measurements included changes of the cytoplasmic Ca2+ concentration ([Ca2+]i), uptake of 45Ca2+ and uptake of Mn2+, a Ca2+ surrogate for Ca2+ channels. Refilling of the Ca2+ stores in thymocytes was very fast (half-filling time: 4 s at 37 degrees C) and very sensitive to temperature (10 times slower at 20 degrees C). It was always preceded by increase of [Ca2+]i. In the Eh

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!