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Journal articles on the topic 'EIS-biosensor'

Author: Grafiati

Published: 1 April 2023

Last updated: 19 July 2025

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1

Li, Jiaxuan, and Jingyi Jiang. "An Electrochemical Impedance Spectroscopy Biosensor for Detecting IgG in Human Urine." Theoretical and Natural Science 78, no. 1 (2025): 111–19. https://doi.org/10.54254/2753-8818/2024.19969.

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IgG (immunoglobulin G) is the most abundant serum antibody found in blood circulation in humans and is not present in healthy human urine. The detection of IgG in urine is closely related to valuating the severity and predicting the trend of glomerulitis and other chronic kidney diseases. Electrochemical impedance spectroscopy (EIS) biosensors are highly sensitive and ideal for detecting IgG in urine. This technique uses micro-gap parallel plate electrode (PPE) as the electrode of the EIS biosensor and antifouling Y-shaped peptides as biometric element combined with PPE. The development of an

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2

Oliveira, A. C., and L. H. Mascaro. "Evaluation of Acetylcholinesterase Biosensor Based on Carbon Nanotube Paste in the Determination of Chlorphenvinphos." International Journal of Analytical Chemistry 2011 (2011): 1–6. http://dx.doi.org/10.1155/2011/974216.

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An amperometric biosensor for chlorphenvinphos (organophosphorus pesticide) based on carbon nanotube paste and acetylcholinesterase enzyme (CNTs-AChE biosensor) is described herein. This CNTs-AChE biosensor was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The SEM result shows the presence of CNTs and small lumps, due to the enzyme AChE, which has a type of cauliflower formation. From EIS analysis is possible to observe increasedRtcfor CNTs-AChE biosensor when compared to the carbon nanotube paste electrode for the reaction [Fe(CN)6]4−/3−

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3

Elnagar, Noha, Nada Elgiddawy, Waleed M. A. El Rouby, Ahmed A. Farghali, and Hafsa Korri-Youssoufi. "Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymers." Biosensors 14, no. 2 (2024): 77. http://dx.doi.org/10.3390/bios14020077.

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The sensitive determination of folate receptors (FRs) in the early stages of cancer is of great significance for controlling the progression of cancerous cells. Many folic acid (FA)-based electrochemical biosensors have been utilized to detect FRs with promising performances, but most were complicated, non-reproducible, non-biocompatible, and time and cost consuming. Here, we developed an environmentally friendly and sensitive biosensor for FR detection. We proposed an electrochemical impedimetric biosensor formed by nanofibers (NFs) of bio-copolymers prepared by electrospinning. The biosensor

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4

Manickam, Arun, Aaron Chevalier, Mark McDermott, Andrew D. Ellington, and Arjang Hassibi. "A CMOS Electrochemical Impedance Spectroscopy (EIS) Biosensor Array." IEEE Transactions on Biomedical Circuits and Systems 4, no. 6 (2010): 379–90. http://dx.doi.org/10.1109/tbcas.2010.2081669.

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5

Le, Hien T. Ngoc, and Sungbo Cho. "Sensitive Electrochemical Detection of Phosphorylated-Tau Threonine 231 in Human Serum Using Interdigitated Wave-Shaped Electrode." Biomedicines 10, no. 1 (2021): 10. http://dx.doi.org/10.3390/biomedicines10010010.

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The development of an electrochemical biosensor for the detection of phosphorylated-tau threonine 231 (p-tau231), a biomarker of Alzheimer’s disease (AD), has yet to be achieved. Therefore, in this study, we developed a simple, small size, cheap, and sensitive electrochemical biosensor based on an interdigitated wave-shaped electrode via an activated self-assembled monolayer to preserve a specific anti–p-tau231 antibody (IWE/SAM/EDC-NHS/anti–p-tau231). Detection of p-tau231 in human serum (HS) using the biosensor was undertaken using electrochemical impedance spectroscopy (EIS). The change in

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6

Zhou, Yaoyu, Lin Tang, Xia Xie, et al. "Sensitive impedimetric biosensor based on duplex-like DNA scaffolds and ordered mesoporous carbon nitride for silver(i) ion detection." Analyst 139, no. 24 (2014): 6529–35. http://dx.doi.org/10.1039/c4an01607f.

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7

Shivabalan, Arun Prabha, Filipas Ambrulevicius, Martynas Talaikis, Vaidas Pudzaitis, Gediminas Niaura, and Gintaras Valincius. "Effect of pH on Electrochemical Impedance Response of Tethered Bilayer Lipid Membranes: Implications for Quantitative Biosensing." Chemosensors 11, no. 8 (2023): 450. http://dx.doi.org/10.3390/chemosensors11080450.

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Tethered bilayer lipid membranes (tBLMs) are increasingly used in biosensor applications where electrochemical impedance spectroscopy (EIS) is the method of choice for amplifying and recording the activity of membrane-damaging agents such as pore-forming toxins or disrupting peptides. While the activity of these biological agents may depend on the pH of the analytes, there is increasing evidence that the sensitivity of tethered bilayer sensors depends on the pH of the solutions. In our study, we addressed the question of what are the fundamental reasons for the variability of the EIS signal of

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8

Gasparyan, L. F., I. A. Mazo, V. V. Simonyan, and F. V. Gasparyan. "EIS Biosensor for Detection of Low Concentration DNA Molecules." Journal of Contemporary Physics (Armenian Academy of Sciences) 55, no. 1 (2020): 101–9. http://dx.doi.org/10.3103/s1068337220010144.

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9

Li, Jing, Byung Kun Kim, Kang-Kyun Wang, et al. "Sensing Estrogen with Electrochemical Impedance Spectroscopy." Journal of Analytical Methods in Chemistry 2016 (2016): 1–6. http://dx.doi.org/10.1155/2016/9081375.

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This study demonstrates the application feasibility of electrochemical impedance spectroscopy (EIS) in measuring estrogen (17β-estradiol) in gas phase. The present biosensor gives a linear response (R2=0.999) for 17β-estradiol vapor concentration from 3.7 ng/L to 3.7 × 10−4 ng/L with a limit of detection (3.7 × 10−4 ng/L). The results show that the fabricated biosensor demonstrates better detection limit of 17β-estradiol in gas phase than the previous report with GC-MS method. This estrogen biosensor has many potential applications for on-site detection of a variety of endocrine disrupting com

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10

Jamshaid, Talha, Ernandes Taveira Tenório-Neto, Abdoullatif Baraket, et al. "Development of Novel Magneto-Biosensor for Sulfapyridine Detection." Biosensors 10, no. 4 (2020): 43. http://dx.doi.org/10.3390/bios10040043.

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In this work, we report the development of a highly sensitive biosensor for sulfapyridine detection based on an integrated bio micro-electromechanical system (Bio-MEMS) containing four gold working electrodes (WEs), a platinum counter electrode (CE), and a reference electrode (RE). Firstly, the cleaned WEs were modified with 4-aminophenylacetic acid (CMA). Then, (5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid (SA2BSA) was immobilized onto the transducers surface by carbodiimide chemistry. The analyte was quantified by competitive detection with SA2BSA immobilized on the WE toward a mixture

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11

Antunes, Rafael Souza, Douglas Vieira Thomaz, Luane Ferreira Garcia, Eric de Souza Gil, Vernon Sydwill Sommerset, and Flavio Marques Lopes. "Determination of Methyldopa and Paracetamol in Pharmaceutical Samples by a Low Cost Genipa americana L. Polyphenol Oxidase Based Biosensor." Advanced Pharmaceutical Bulletin 9, no. 3 (2019): 416–22. http://dx.doi.org/10.15171/apb.2019.049.

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Purpose: Jenipapo fruit (Genipa americana L) is a natural source of polyphenol oxidases (PPOs) whose potential in pharmaceutical analysis is noteworthy. Henceforth, this work reports the electrochemical study of a low-cost PPO-based biosensor produced from the crude extract of Jenipapo fruits and accounts a practical approach to employ this biosensor in the determination of methyldopa and paracetamol in pharmaceutical samples. Methods: In order to investigate the electrochemical properties of the biosensor, theoretical and practical approaches were employed, and both samples and the biosensor

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12

Cheng, Tzong-Jih, Hsien-Yi Hsiao, Pei-Chia Tsai, and Richie L. C. Chen. "Redoxless Electrochemical Capacitance Spectroscopy for Investigating Surfactant Adsorption on Screen-Printed Carbon Electrodes." Chemosensors 11, no. 6 (2023): 343. http://dx.doi.org/10.3390/chemosensors11060343.

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Electrochemical impedance spectroscopy (EIS) is a sensitive analytical method for surface and bulk properties. Classical EIS and derived electrochemical capacitance spectroscopy (ECS) with a redox couple are label-free approaches for biosensor development, but doubts arise regarding interpretability when a redox couple is employed (redox EIS) due to interactions between electroactive probes and interfacial charges or forced potential. Here, we demonstrated redoxless ECS for directly determining surfactant adsorption on screen-printed carbon electrodes (SPCEs), validated through a simulation of

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13

Yang, Jie, Dawei Li, Zengyuan Pang, and Qufu Wei. "Laccase Biosensor Based on Ag-Doped TiO2 Nanoparticles on CuCNFs for the Determination of Hydroquinone." Nano 11, no. 12 (2016): 1650132. http://dx.doi.org/10.1142/s1793292016501320.

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A novel nanomaterial composed of copper and carbon nanofibers (CuCNFs) decorated with Ag-doped TiO2 (Ag–TiO[Formula: see text] nanoparticles was prepared through electrospinning, carbonization and solvothermal treatment. The composites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and electrochemical impedance spectroscopy (EIS). The obtained composites were mixed with laccase and Nafion to construct novel hydroquinone biosensor. The electrochemical behavior of the novel

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14

Virbickas, Povilas, Narvydas Dėnas, and Aušra Valiūnienė. "Electrochemical Mercury Biosensor Based on Electrocatalytic Properties of Prussian Blue and Inhibition of Catalase." Chemosensors 11, no. 5 (2023): 311. http://dx.doi.org/10.3390/chemosensors11050311.

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This paper presents a detailed study of a novel type of electrochemical mercury ion (Hg2+) biosensor developed by combining Prussian blue (PB) and catalase (Cat). The simultaneous PB-catalyzed reduction of hydrogen peroxide and the inhibition of catalase by Hg2+ ions were used as the working principle of the biosensor. The biosensor described in this research was capable of detecting Hg2+ ions at relatively low potentials (+0.2 V vs. Ag|AgCl, KClsat) using chronoamperometry and a fast Fourier transform electrochemical impedance spectroscopy (FFT-EIS). Linear ranges of 0.07 mM–3 mM and 0.13 mM–

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15

Zhou, Yong. "Novel Approach to Fabricate an Amperometric Biosensor for Glucose Based on Nafion, Azure І and Gold Nanoparticles". Advanced Materials Research 345 (вересень 2011): 331–33. http://dx.doi.org/10.4028/www.scientific.net/amr.345.331.

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A novel approach to fabricate an amperometric biosensor for the detection of glucose was described. By means of self-assembled technique and the opposite-charged adsorption, Nafion(Naf), Azure І (Azu), gold nanoparticles (nano-Au) and glucose oxidase (GOD) were immobilized on the gold electrode subsequently. The GOD immobilized on the nano-Au monolayer was stable and retained its functional activity. The stepwise self-assemble procedure of the immunosensor was further characterized by means of cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The factors influencing the

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16

Nycz, Marta, Katarzyna Arkusz, and Dorota G. Pijanowska. "Fabrication of Electrochemical Biosensor Based on Titanium Dioxide Nanotubes and Silver Nanoparticles for Heat Shock Protein 70 Detection." Materials 14, no. 13 (2021): 3767. http://dx.doi.org/10.3390/ma14133767.

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This paper presents the fabrication methodology of an electrochemical biosensor for the detection of heat shock protein 70 (HSP70) as a potential tumor marker with high diagnostic sensitivity. The sensor substrate was a composite based on titanium dioxide nanotubes (TNTs) and silver nanoparticles (AgNPs) produced directly on TNTs by electrodeposition, to which anti-HSP70 antibodies were attached by covalent functionalization. This manuscript contains a detailed description of the production, modification, and the complete characteristics of the material used as a biosensor platform. As-formed

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17

Štukovnik, Zala, Regina Fuchs Godec, and Urban Bren. "The Use of Yeast Saccharomyces Cerevisiae as a Biorecognition element in the Development of a Model Impedimetric Biosensor for Caffeine Detection." Acta Chimica Slovenica 69, no. 2 (2022): 378–84. http://dx.doi.org/10.17344/acsi.2021.7301.

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In the present study, an electrochemical-impedimetric biosensor using Saccharomyces cerevisiae as an effective biorecognition element was designed to detect caffeine. The presented biosensor consists of a previously developed stainless steel electrochemical cell constructed as a three-electrode system in the RCW side-by-side configuration. The electrochemical stability of the sensing electrode was evaluated by measuring the open circuit potential (OCP), and electrochemical impedance spectroscopy (EIS) was applied to determine the impedimetric response of the biosensor with Saccharomyces cerevi

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18

Davidson, Chloé E. D., and Ravi Prakash. "Stamp-Imprinted Polymer EIS Biosensor for Amyloid-Beta Detection: A Novel Approach Towards Alzheimer’s Screening." Biosensors 15, no. 4 (2025): 228. https://doi.org/10.3390/bios15040228.

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Surface-imprinted polymers (SIPs) represent an exciting and cost-effective alternative to antibodies for electrochemical impedance spectroscopy (EIS)-based biosensing. They can be produced using simple printing techniques and have shown high efficacy in detecting large biomolecules and microorganisms. Stamp imprinting, a novel SIP method, creates the target analyte’s imprint using a soft lithography mask of the analyte matrix, thereby reducing material complexities and eliminating the need for cross-linking, which makes the process more scalable than the conventional SIPs. In this work, we dem

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19

Kesici, Ece, Ece Eksin, and Arzum Erdem. "An Impedimetric Biosensor Based on Ionic Liquid-Modified Graphite Electrodes Developed for microRNA-34a Detection." Sensors 18, no. 9 (2018): 2868. http://dx.doi.org/10.3390/s18092868.

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In the present work, an impedimetric nucleic acid biosensor has been designed for the purpose of detection of microRNA (miRNA). Ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate (IL))-modified chemically activated pencil graphite electrodes (PGEs) were used for the sensitive and selective detection of miRNA-34a. After covalent activation of the PGE surface using covalent agents (CAs), the ionic liquid (IL) was immobilized onto the surface of the chemically activated PGE by passive adsorption. The electrochemical and microscopic characterization of the IL/CA/PGEs was performed by el

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20

Lai, Huat Choi, Suk Fun Chin, Suh Cem Pang, Magdline Sia Henry Sum, and David Perera. "Carbon Nanoparticles Based Electrochemical Biosensor Strip for Detection of Japanese Encephalitis Virus." Journal of Nanomaterials 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/3615707.

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We reported a disposable and sensitive electrochemical biosensor strip based on carbon nanoparticles modified screen-printed carbon electrode (SPCE) for rapid and sensitive detection of Japanese Encephalitis Virus (JEV). Amino group functionalized carbon nanoparticles were prepared from preformed chitosan nanoparticles. Japanese Encephalitis Virus (JEV) antibody was immobilized onto the surfaces of carbon nanoparticles through amide bonds formation between amino groups of carbon nanoparticles and carboxylic groups of JEV antibody. The analytical performance of SPCE electrochemical biosensor st

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21

Cui, Han, Zhao Hao Wang, Qi Jin Wan, and Nian Jun Yang. "Self-Assembled Au Electrode for Direct Electrochemistry of Horseradish Peroxidase and Detection of Hydrogen Peroxide." Advanced Materials Research 704 (June 2013): 72–76. http://dx.doi.org/10.4028/www.scientific.net/amr.704.72.

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The H2O2 biosensor was prepared by continuous processes: 2,3-dimercaptosuccinic acid (DMSA) self-assembly monolayers gold nanoparticles (AuNPs) film by electrodepositioncovalent immobilization of horseradish peroxidase (HRP). In pH 7.2 phosphoric buffer solution (PBS), the HRP-AuNPs-DMSA-Au electrode exhibited a pair of well-defined cyclic voltammetric peaks in H2O2 solution, and the oxidation peak is about +0.4V versus saturated calomel electrode (SCE). The resulting substrates were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The biosensor exhibi

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22

Zaid, Mohd Hazani Mat, Jaafar Abdullah, Nor Azah Yusof, et al. "Reduced Graphene Oxide/TEMPO-Nanocellulose Nanohybrid-Based Electrochemical Biosensor for the Determination of Mycobacterium tuberculosis." Journal of Sensors 2020 (January 21, 2020): 1–11. http://dx.doi.org/10.1155/2020/4051474.

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A novel peptide nuclide acid (PNA) electrochemical biosensor based on reduced graphene oxide (NH2-rGO)/2,2,6,6-tetramethylpiperidin-1-yl)oxyl nanocrystalline cellulose (TEMPO-NCC) for the detection of Mycobacterium tuberculosis (M. Tuberculosis) is described. In this study, the nanohybrid films NH2-rGO/TEMPO-NCC were immobilized onto screen-printed carbon electrode (SPE) via a simple drop-coating method. The electrochemical characterization of the designed electrode was investigated using cyclic voltammetry (CV) and impedance spectroscopy (EIS). Meanwhile, the sensitivity and selectivity of th

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23

Lee, So-Ra, Hyo-Eun Lee, Yun Ok Kang, Wan-Seok Hwang, and Seong-Ho Choi. "BienzymaticAcetylcholinesteraseandCholine OxidaseImmobilized Biosensor Based on a Phenyl Carboxylic Acid-Grafted Multiwalled Carbon Nanotube." Advances in Materials Science and Engineering 2014 (2014): 1–12. http://dx.doi.org/10.1155/2014/971942.

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Bienzymaticacetylcholinesterase(AChE) andcholine oxidase(ChOx) immobilized biosensor based on a phenyl carboxylic acid-grafted multiwalled carbon nanotube (MWNT) modified glass carbon electrode (GCE) and carbon-screen printed electrode (SPE) was fabricated for acetylcholine detection in human blood samples. Phenyl carboxylic acid-modified MWNT supports were prepared by electrochemical polymerization of 4-carboxyphenyl diazonium salts, which were synthesized by an amine group and sodium nitrite, on the surface of the MWNT-modified GCE and SPE in 0.1 M PBS. The successful fabrication of the AChE

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24

Jiang, Nianyu, and Pranav Shrotriya. "Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane." Micromachines 16, no. 1 (2024): 35. https://doi.org/10.3390/mi16010035.

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We report a low-cost, portable biosensor composed of an aptamer-functionalized nanoporous anodic aluminum oxide (NAAO) membrane and a commercial microcontroller chip-based impedance reader suitable for electrochemical impedance spectroscopy (EIS)-based sensing. The biosensor consists of two chambers separated by an aptamer-functionalized NAAO membrane, and the impedance reader is utilized to monitor transmembrane impedance changes. The biosensor is utilized to detect amodiaquine molecules using an amodiaquine-binding aptamer (OR7)-functionalized membrane. The aptamer-functionalized membrane is

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25

Wu, Hai Yun, Xiao Ying Guo, Ya Ping Yu, et al. "Rapid Quantitative Detection of E.coli O157:H7 by a Impedance Immunosensor Based on Four-Wire Interdigitated Microelectrodes." Applied Mechanics and Materials 738-739 (March 2015): 111–15. http://dx.doi.org/10.4028/www.scientific.net/amm.738-739.111.

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Rapid-response biosensing systems are necessary to counteract threats due to high-consequence pathogens. A disposable immunosensor suitable for quantitative determination of E.coli O157:H7 in pure cultures was investigated by electrochemical impedance spectroscopy (EIS). Electric responses of this biosensor for different cell concentrations at multi-frequencies were explored utilizing disposable screen-printed silver four-wire interdigitated microelectrodes (IMEs). Additionally, the best response frequency for the detection was studied, and the relationship between the impedance at this freque

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26

Irani, Koosha, Hossein Siampour, Abdollah Allahverdi, Ahmad Moshaii, and Hossein Naderi-Manesh. "Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer Screening." International Journal of Molecular Sciences 24, no. 24 (2023): 17225. http://dx.doi.org/10.3390/ijms242417225.

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Lung cancer is one of the deadliest cancers worldwide due to the inability of existing methods for early diagnosis. Tumor-derived exosomes are nano-scale vesicles released from tumor cells to the extracellular environment, and their investigation can be very useful in both biomarkers for early cancer screening and treatment assessment. This research detected the exosomes via an ultrasensitive electrochemical biosensor containing gold nano-islands (Au-NIs) structures. This way, a high surface-area-to-volume ratio of nanostructures was embellished on the FTO electrodes to increase the chance of

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Štukovnik, Zala, Urban Bren, and Bren Rozman. "Model Electrochemical Biosensor for the Detection of Methanol in Aqueous Solutions with Yeast Cells." Acta Chimica Slovenica 68, no. 4 (2021): 773–80. http://dx.doi.org/10.17344/acsi.2020.6545.

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An electrochemical device that serves as a model biosensor and contains yeast Saccharomyces cerevisiae as the active biological element was developed. Different configurations of the electrochemical cells were assembled and tested. Stainless steel was used in the electrochemical cell composition process and the surface of this metal electrode was modified with a thin layer of WO3 if necessary. The yeast Saccharomyces cerevisiae was adhered to the working electrode. The resulting model biosensor was then used to monitor the response to a 10% CH3OH. For detection of biological activity, the elec

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Sedlackova, Richtera, Estrela, and Adam. "Effect of Graphene Oxide Modification on a DNA Biosensor Developed for the Detection of Methylated DNA Associated with Cancer." Proceedings 15, no. 1 (2019): 13. http://dx.doi.org/10.3390/proceedings2019015013.

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Methylated DNA is a covalent post-translational modification, which plays a crucial role in pathological and physiological processes including several diseases, such as cardiovascular disease, diabetes or cancer. Despite that, methylated DNA presents a new generation of biomarkers, which brings a promising alternative for using in point-care diagnostic. Regarding this fact, DNA based electrochemical sensors enable fast, reliable, low-cost, time-consuming and efficient detection. The application of these biosensors as possible alternatives for the determination of methylated DNA is recently gro

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Meshesha, Mesfin, Anik Sardar, Ruchi Supekar, et al. "Development and Analytical Evaluation of a Point-of-Care Electrochemical Biosensor for Rapid and Accurate SARS-CoV-2 Detection." Sensors 23, no. 18 (2023): 8000. http://dx.doi.org/10.3390/s23188000.

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The COVID-19 pandemic has underscored the critical need for rapid and accurate screening and diagnostic methods for potential respiratory viruses. Existing COVID-19 diagnostic approaches face limitations either in terms of turnaround time or accuracy. In this study, we present an electrochemical biosensor that offers nearly instantaneous and precise SARS-CoV-2 detection, suitable for point-of-care and environmental monitoring applications. The biosensor employs a stapled hACE-2 N-terminal alpha helix peptide to functionalize an in situ grown polypyrrole conductive polymer on a nitrocellulose m

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Yu, Zhixue, Hui Wang, Yiguang Zhao, Fan Zhang, Xiangfang Tang, and Benhai Xiong. "Electrochemical Biosensor Using Nitrogen-Doped Graphene/Au Nanoparticles/DNAzyme for Ca2+ Determination." Biosensors 12, no. 5 (2022): 331. http://dx.doi.org/10.3390/bios12050331.

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An electrochemical biosensor for detecting Ca2+ concentration was proposed using glass carbon electrodes (GCEs) modified with nitrogen-doped graphene (NGR), gold nanoparticles (AuNPs) and DNAzyme. The resistance signal was amplified through two methods: electrochemical reduction of AuNPs on the NGR surface to increase the specific surface area of the electrode and strengthen the adsorption of DNAzyme; and increasement of the DNAzyme base sequence. The process of electrode modification was characterized by scanning electron microscopy, Raman spectroscopy, cyclic voltammetry (CV), and electroche

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Huang, Zhiheng, Chunchuan Gu, Jiajun Wen, Langlang Zhu, Mingzhen Zhang, and Hongying Liu. "One-Pot Electrodeposition of NiS Nanoparticles as an Efficient Electrode for Nonenzymatic H2O2 and Glucose Sensors." Nano 14, no. 01 (2019): 1950010. http://dx.doi.org/10.1142/s1793292019500103.

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In this paper, a new and one-pot electrodeposition method was expanded for the preparation of NiS nanoparticles-based electrochemical biosensor using metal-ion complexes as a precursor. Thioacetamide was used to control the production rate of NiS nanoparticles for the first time. The proposed electrochemical sensor was characterized by energy dispersive X-ray spectroscopy (EDX), field emission scanning electron microscope (FESEM), cyclic voltammograms (CV), and electrochemical impedance spectra (EIS). Experiment parameters were optimized. Under the optimized condition, the prepared NiS-based b

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Suganthan, Baviththira, Ashley M. Rogers, Clay S. Crippen, et al. "A Bacteriophage Protein-Based Impedimetric Electrochemical Biosensor for the Detection of Campylobacter jejuni." Biosensors 14, no. 8 (2024): 402. http://dx.doi.org/10.3390/bios14080402.

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Campylobacter jejuni is a common foodborne pathogen found in poultry that can cause severe life-threatening illnesses in humans. It is important to detect this pathogen in food to manage foodborne outbreaks. This study reports a novel impedimetric phage protein-based biosensor to detect C. jejuni NCTC 11168 at 100 CFU/mL concentrations using a genetically engineered receptor-binding phage protein, FlaGrab, as a bioreceptor. The electrochemical impedance spectroscopy (EIS) technique was employed to measure changes in resistance upon interaction with C. jejuni. The sensitivity of the phage prote

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Negahdary, Masoud, Mahnaz Jafarzadeh, Roya Rahimzadeh, Ghasem Rahimi, and Hamideh Dehghani. "A DNA biosensor for molecular diagnosis of <i>Aeromonas hydrophila</i> using zinc sulfide nanospheres." Journal of Sensors and Sensor Systems 6, no. 2 (2017): 259–67. http://dx.doi.org/10.5194/jsss-6-259-2017.

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Abstract. Today, identification of pathogenic bacteria using modern and accurate methods is inevitable. Integration in electrochemical measurements with nanotechnology has led to the design of efficient and sensitive DNA biosensors against bacterial agents. Here, efforts were made to detect Aeromonas hydrophila using aptamers as probes and zinc sulfide (ZnS) nanospheres as signal enhancers and electron transfer facilitators. After modification of the working electrode area (in a screen-printed electrode) with ZnS nanospheres through electrodeposition, the coated surface of a modified electrode

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Tortolini, Cristina, Valeria Gigli, Flavio Rizzo, et al. "Stereoselective Voltammetric Biosensor for Myo-Inositol and D-Chiro-Inositol Recognition." Sensors 23, no. 22 (2023): 9211. http://dx.doi.org/10.3390/s23229211.

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This paper describes the development of a simple voltammetric biosensor for the stereoselective discrimination of myo-inositol (myo-Ins) and D-chiro-inositol (D-chiro-Ins) by means of bovine serum albumin (BSA) adsorption onto a multi-walled carbon nanotube (MWCNT) graphite screen-printed electrode (MWCNT-GSPE), previously functionalized by the electropolymerization of methylene blue (MB). After a morphological characterization, the enantioselective biosensor platform was electrochemically characterized after each modification step by differential pulse voltammetry (DPV) and electrochemical im

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Liang, Jintao, Shuai Zhang, Qing Huang, Guiyin Li, and Zhide Zhou. "Application of Nitrogen-Doped Reduced Graphene Oxide-Persimmon Tannin Nanocomposites for Electrochemical Detection of Cd (II) in Water Resources." Journal of The Electrochemical Society 171, no. 2 (2024): 027508. http://dx.doi.org/10.1149/1945-7111/ad2314.

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An ultrasensitive electrochemical biosensor for the detection of Cadmium (Cd(II)) was successfully constructed based on nitrogen-doped reduced graphene oxide-persimmon tannin (PT-N-RGO) nanocomposite and gold nanoparticles (Au NPs) modified screen-printed electrode (SPE). The structure and morphology of the prepared PT-N-RGO nanocomposite were characterized by ultraviolet-visible spectroscopy (UV–vis), fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Each step for the electroch

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Choowongkomon, Kiattawee, Janeyuth Chaisakul, Supaphorn Seetaha, et al. "Development of a Biosensor to Detect Venom of Malayan Krait (Bungarus candidus)." Toxins 16, no. 1 (2024): 56. http://dx.doi.org/10.3390/toxins16010056.

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Malayan krait (Bungarus candidus) envenoming is a cause of significant morbidity and mortality in many Southeast Asian countries. If intubation and specific antivenom administration are delayed, the most significant life-threatening outcome may be the inhibition of neuromuscular transmission and subsequent respiratory failure. It is recommended that krait-envenomed victims without indications of neurotoxicity, e.g., skeletal muscle weakness or ptosis, immediately receive 10 vials of antivenom. However, the administration of excess antivenom may lead to hypersensitivity or serum sickness. There

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Pundir, Chandra S., Vinay Narwal, Parveen Kumar, and Deepa. "An Enhanced Amperometric Triglyceride Biosensor by Graphene Oxide Nanoparticles." International Journal of Applied Sciences and Biotechnology 13, no. 1 (2025): 1–11. https://doi.org/10.3126/ijasbt.v13i1.76786.

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An enhanced amperometric triglyceride (TG) biosensor was created by electrodepositing graphene oxide nanoparticles (GrONPs) onto pencil graphite electrode (PGE) and then co-immobilizing enzyme nanoparticles (ENPs) of lipase, glycerol kinase nanoparticles (GKNPs), and glycerol 3-oxidase (GPONPs), onto this modified electrode. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry, and scanning electron microscopy (SEM) were employed to examine the working electrode (ENPs/GrONPs/PGE) at various phases of its creation. At an applied potential of 0.3V, the biosensor showed its best curre

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Samy, Mostafa Ahmed, Muhammed Abdel-Hamied Abdel-Tawab, Nour T. Abdel-Ghani, and Rasha M. El Nashar. "Application of Molecularly Imprinted Microelectrode as a Promising Point-of-Care Biosensor for Alanine Aminotransferase Enzyme." Chemosensors 11, no. 5 (2023): 262. http://dx.doi.org/10.3390/chemosensors11050262.

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Alanine amino transaminase (ALT) is an enzyme that can be used as a biomarker for liver injury and other diseases. In this work, we report the development of the first microelectrode based on a molecularly imprinted pyruvate oxidase enzyme to be applied as an electrochemical biosensor for ALT detection. The biosensor is based on pyruvate oxidase enzyme (POx), imprinted using 4-aminophenol (functional monomer-on-platinum microelectrode modified (PME)) with platinum nanoparticles and 4-aminoantypirine (4-AAP)/sodium pyruvate as an electrochemical indicator. The operational conditions of the bios

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Soysaldı, Furkan, Derya Dincyurek Ekici, Mehmet Çağrı Soylu, and Evren Mutlugun. "Electrochemical and Optical Multi-Detection of Escherichia coli Through Magneto-Optic Nanoparticles: A Pencil-on-Paper Biosensor." Biosensors 14, no. 12 (2024): 603. https://doi.org/10.3390/bios14120603.

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Escherichia coli (E. coli) detection suffers from slow analysis time and high costs, along with the need for specificity. While state-of-the-art electrochemical biosensors are cost-efficient and easy to implement, their sensitivity and analysis time still require improvement. In this work, we present a paper-based electrochemical biosensor utilizing magnetic core-shell Fe2O3@CdSe/ZnS quantum dots (MQDs) to achieve fast detection, low cost, and high sensitivity. Using electrochemical impedance spectroscopy (EIS) as the detection technique, the biosensor achieved a limit of detection of 2.7 × 10

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Kumar, Neeraj, Shalu Yadav, Mohd Abubakar Sadique, and Raju Khan. "Electrochemically Exfoliated Graphene Quantum Dots Based Biosensor for CD44 Breast Cancer Biomarker." Biosensors 12, no. 11 (2022): 966. http://dx.doi.org/10.3390/bios12110966.

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An innovative electrochemical biosensor based on graphene quantum dots (GQDs) is developed for a simple, rapid, and highly sensitive primary diagnosis of the breast cancer biomarker cluster of differentiation-44 (CD44) antigen. Herein, electrochemical exfoliation of waste dry batteries provides facile, eco-friendly, and cost-effective synthesis of GQDs. Transmission electron microscopy (TEM) analysis reveals that GQDs exhibit spherical shapes with an average diameter of 4.75 nm. Further, electrochemical analysis through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) r

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Ganguly, Antra, Anirban Paul, and Shalini Prasad. "Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing." Chemosensors 11, no. 11 (2023): 557. http://dx.doi.org/10.3390/chemosensors11110557.

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Drawing inspiration from the several thousand beautiful Pysanky egg art of Ukraine, we have developed a novel material, Aptamer–Gold Nanoparticles (AuNPs)@ZIF-8, that can be used for building sensitive and highly stable POC biosensors for longitudinal health mapping. Here, we demonstrate a sensitive and specific novel electrochemical biosensor, made of a novel synthesized in situ encapsulated aptamer-AuNPs@ZIF-8 composite, for monitoring levels of creatinine (0.1–1000 μg/mL). In this work, we have reported the synthetic protocol for the first-of-a-kind in situ encapsulation of aptamer and AuNP

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Rozman, Martin, Zala Štukovnik, Ajda Sušnik, et al. "A HepG2 Cell-Based Biosensor That Uses Stainless Steel Electrodes for Hepatotoxin Detection." Biosensors 12, no. 3 (2022): 160. http://dx.doi.org/10.3390/bios12030160.

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Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue (CBB)

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Rooma, Devi 1* S.K. Tripathi 1. Aman Chauhan2 &. Manju Bala2. "HIGHLY SENSITIVE AND RAPID DETECTION OF PUTRESCINE BY USING AN PLATINUM ELECTRODE MODIFIED WITH ZINCOXIDE NANOPARTICLES-POLYPYRROLE COMPOSITE." GLOBAL JOURNAL OF ENGINEERING SCIENCE AND RESEARCHES 5, no. 11 (2018): 13–26. https://doi.org/10.5281/zenodo.1544308.

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A highly selective, sensitive, specific and rapid electrochemical putrescine biosensor was constructed by covalently immobilizing diamine oxidase purified from<em> Pisum sativum  </em>leaves on polypyrrole 3-carboxylic acid (PPy-3C) with zinc oxide nanoparticles ( ZnONps) using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) modified platinum electrode.Transmission electron microscopy (TEM), X-ray diffraction (XRD), UV spectroscopy and Dynamic light scattering (DLS) were used for characterization of ZnONps. The modified enzyme electrode was characteriz

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Maâtouk, Ferdaous, Mouna Maâtouk, Karima Bekir, Houcine Barhoumi, Abderrazak Maaref, and Hedi Ben Mansour. "An electrochemical DNA biosensor for trace amounts of mercury ion quantification." Journal of Water and Health 14, no. 5 (2016): 808–15. http://dx.doi.org/10.2166/wh.2016.293.

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In this work we report the development of an electrochemical DNA biosensor with high sensitivity for mercury ion detection. A new matrix based on gold nanoparticles (AuNPs)-glutathione (GSH)/cysteine was investigated. The interaction between DNA oligonucleotides and Hg2+ ions followed by the formation of Thymine–Hg2+–Thymine (T–Hg2+–T) structures was quantified using different electrochemical methods. It has been shown that the electrochemical impedance spectroscopy (EIS) measurements and the differential pulse voltammetry (DPV) confirmed the specific interaction between the oligonucleotide re

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Zhang, Yuzhong, Zhen Wang, Yuehong Wang, Lei Huang, Wei Jiang, and Mingzhu Wang. "Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles." International Journal of Electrochemistry 2011 (2011): 1–7. http://dx.doi.org/10.4061/2011/619782.

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A sensitive electrochemical DNA biosensor was prepared based on mercaptoacetic acid (MAA)/gold nanoparticles (AuNPs) modified electrode. Probe DNA (NH2-DNA) was covalently linked to the carboxyl group of MAA in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxyl-succinimide (NHS). Scanning electron microscopy (SEM) and electrochemical impedance spectra (EIS) were used to investigate the film assembly process. The DNA hybridization events were monitored by differential pulse voltammetry (DPV), and adriamycin was used as the electrochemical indicator

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Chou, Jung-Chuan, Si-Hong Lin, Tsu-Yang Lai, et al. "A Facile Fabrication of a Potentiometric Arrayed Glucose Biosensor Based on Nafion-GOx/GO/AZO." Sensors 20, no. 4 (2020): 964. http://dx.doi.org/10.3390/s20040964.

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In this study, the potentiometric arrayed glucose biosensors, which were based on zinc oxide (ZnO) or aluminum-doped zinc oxide (AZO) sensing membranes, were fabricated by using screen-printing technology and a sputtering system, and graphene oxide (GO) and Nafion-glucose oxidase (GOx) were used to modify sensing membranes by using the drop-coating method. Next, the material properties were characterized by using a Raman spectrometer, a field-emission scanning electron microscope (FE-SEM), and a scanning probe microscope (SPM). The sensing characteristics of the glucose biosensors were measure

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Di Mari, Gisella M., Mario Scuderi, Giuseppe Lanza, et al. "Pain-Free Alpha-Synuclein Detection by Low-Cost Hierarchical Nanowire Based Electrode." Nanomaterials 14, no. 2 (2024): 170. http://dx.doi.org/10.3390/nano14020170.

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Analytical methods for the early detection of the neurodegenerative biomarker for Parkinson’s disease (PD), α-synuclein, are time-consuming and invasive, and require skilled personnel and sophisticated and expensive equipment. Thus, a pain-free, prompt and simple α-synuclein biosensor for detection in plasma is highly demanded. In this paper, an α-synuclein electrochemical biosensor based on hierarchical polyglutamic acid/ZnO nanowires decorated by gold nanoparticles, assembled as nanostars (NSs), for the determination of α-synuclein in human plasma is proposed. ZnO NSs were prepared by chemic

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Marrugo-Ramírez, Jose, Montserrat Rodríguez-Núñez, M. Pilar Marco, Mónica Mir, and Josep Samitier. "Kynurenic Acid Electrochemical Immunosensor: Blood-Based Diagnosis of Alzheimer’s Disease." Biosensors 11, no. 1 (2021): 20. http://dx.doi.org/10.3390/bios11010020.

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Alzheimer’s disease (AD) is a neurodegenerative disorder, characterized by a functional deterioration of the brain. Currently, there are selected biomarkers for its diagnosis in cerebrospinal fluid. However, its extraction has several disadvantages for the patient. Therefore, there is an urgent need for a detection method using sensitive and selective blood-based biomarkers. Kynurenic acid (KYNA) is a potential biomarker candidate for this purpose. The alteration of the KYNA levels in blood has been related with inflammatory processes in the brain, produced as a protective function when neuron

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Chiriacò, Maria, Ilaria Parlangeli, Fausto Sirsi, Palmiro Poltronieri, and Elisabetta Primiceri. "Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes." Electronics 7, no. 12 (2018): 347. http://dx.doi.org/10.3390/electronics7120347.

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A great improvement in food safety and quality controls worldwide has been achieved through the development of biosensing platforms. Foodborne pathogens continue to cause serious outbreaks, due to the ingestion of contaminated food. The development of new, sensitive, portable, high-throughput, and automated platforms is a primary objective to allow detection of pathogens and their toxins in foods. Listeria monocytogenes is one common foodborne pathogen. Major outbreaks of listeriosis have been caused by a variety of foods, including milk, soft cheeses, meat, fermented sausages, poultry, seafoo

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Işın, Deniz, Ece Eksin, and Arzum Erdem. "Graphene-Oxide and Ionic Liquid Modified Electrodes for Electrochemical Sensing of Breast Cancer 1 Gene." Biosensors 12, no. 2 (2022): 95. http://dx.doi.org/10.3390/bios12020095.

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Graphene-oxide and ionic liquid composite-modified pencil graphite electrodes (GO-IL-PGEs) were developed and used as a sensing platform for breast cancer 1 (BRCA1) gene detection. The characterization of GO-IL modified electrodes was executed by scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The nucleic-acid hybridization was monitored by a differential pulse voltammetry (DPV) technique by directly measuring the guanine oxidation signal without using any indicator. The effects of the IL concentration, the probe concentration, and

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