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Dissertations / Theses on the topic 'Elastic line'

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1

Thavalingham, Appapillai. "Deterimination of optimal yield line patterns governing the collapse of slabs." Thesis, Queen's University Belfast, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336673.

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2

Petri, Steven Walter. "The response of line-stiffened fluid-loaded infinite elastic plates to convecting pressure fields." Thesis, Massachusetts Institute of Technology, 1987. http://hdl.handle.net/1721.1/14347.

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3

Shin, Jong Gye. "Three-dimensional simulation of thermo elastic plastic plate bending by the line heating method." Thesis, Massachusetts Institute of Technology, 1989. http://hdl.handle.net/1721.1/14480.

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4

Makhavikou, Valery [Verfasser], and Roland [Akademischer Betreuer] Kasper. "Line-fitting method of model order reduction for elastic multibody systems / Valery Makhavikou. Betreuer: Roland Kasper." Magdeburg : Universitätsbibliothek, 2015. http://d-nb.info/1079901450/34.

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5

Knabe, Coleman Scott. "Design of Linear Series Elastic Actuators for a Humanoid Robot." Thesis, Virginia Tech, 2015. http://hdl.handle.net/10919/53511.

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Series elastic actuators (SEAs) have numerous benefits for force controlled robotic applications. This thesis presents the design and assembly of a set of compact, lightweight, low-friction linear SEAs for the legs of the Tactical Hazardous Operations Robot (THOR). The THOR SEA pairs a ball screw driven linear actuator with a configurable titanium leaf spring. A removable pivot changes the effective cantilever length, setting the compliance to either 372 or 655 kN/m. Unlike typical SEAs which measure actuator load through spring deflection, an in-line axial load cell directly measures actuator
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6

Ahart, Stephen Gregory. "Yield-line analysis and experimental study of reinforced concrete slabs containing openings." Thesis, Virginia Polytechnic Institute and State University, 1986. http://hdl.handle.net/10919/101267.

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Four rectangular, isotropically reinforced concrete slabs were constructed and loaded until collapse. All slabs were fixed on three edges with the fourth edge free. Three slabs contained openings at various locations while the fourth remained solid. The magnitudes of deflections were measured during loading and the final yield pattern and ultimate load were compared to those predicted by simple and advanced yield-line theory. An analytical computer program was developed and is presented for quick evaluation of the ultimate load and collapse mode of many types of uniformly loaded slabs by sim
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7

Gür, Yüksel. "Part I--Mean and fluctuating wall shear stress measurements ; Part II--Fluid-loaded elastic slab undergoing line and point load excitations." Thesis, Massachusetts Institute of Technology, 1993. http://hdl.handle.net/1721.1/11982.

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8

Олійник, Я. О., Іван Володимирович Павленко, Иван Владимирович Павленко та Ivan Volodymyrovych Pavlenko. "Дослідження прогину балки шляхом розв'язання неленійного рівняння пружної осі". Thesis, Сумський державний університет, 2017. http://essuir.sumdu.edu.ua/handle/123456789/65093.

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Процес проектування машинобудівних конструкцій нерозривно пов’язаний із дослідженням їх напружено-деформованого стану. Ураховуючи, що переважна кількість конструкцій складається з елементів, що працюють на згинання, то задача їх розрахунку є актуальною.
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9

Votsios, Vasilis. "Contact mechanics and impact dynamics of non-conforming elastic and viscoelastic semi-infinite or thin bonded layered solids." Thesis, Loughborough University, 2003. https://dspace.lboro.ac.uk/2134/11815.

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The thesis is concerned with the contact mechanics behaviour of non-conforming solids. The geometry of the solids considered gives rise to various contact configurations, from concentrated contacts with circular and elliptical configuration to those of finite line nature, as well as those of less concentrated form such as circular flat punches. The radii of curvature of mating bodies in contact or impact give rise to these various nonconforming contact configurations and affect their contact characteristics, from those considered as semi-infinite solids in accord with the classical Hertzian th
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10

Wolf, Erich N. "Pressure broadening and pressure shift of diatomic iodine at 675 nm." Thesis, Connect to title online (Scholars' Bank) Connect to title online (ProQuest), 2009. http://hdl.handle.net/1794/10267.

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11

Shcherban, V. Yu. "Structure of the computer program of realization algorithm implementation." Thesis, Київський національний університет технологій та дизайну, 2020. https://er.knutd.edu.ua/handle/123456789/16486.

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12

Tjörnhammar, Richard. "Classical and Quantum Descriptions of Proteins, Lipids and Membranes." Doctoral thesis, KTH, Teoretisk biologisk fysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-151396.

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In this thesis the properties of proteins and membranes are studied by molecular dynamics simulations. The subject is decomposed into parts addressing free energy calculations in proteins, mechanical inclusion models for lipid bilayers, phase transitions and structural correlations in lipid bilayers and atomistic lipid bilayer models. The work is based on results from large scale computer simulations, quantum mechanical and continuum models. Efficient statistical sampling and the coarseness of the models needed to describe the ordered and disordered states are of central concern. Classical fre
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13

Soubsol, David. "Diffusion acoustique vers l'avant d'objets élastiques immergés dans l'eau." Thesis, Normandie, 2018. http://www.theses.fr/2018NORMLH17/document.

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L’étude de la diffusion acoustique pour des objets sphériques et de forme LINE (cylindre terminé à chacune de ses extrémités par une calotte hémisphérique) pleins et immergés dans l’eau en configuration bistatique est l’objet de ce mémoire. Une attention particulière est portée à la diffusion vers l’avant par ces objets. L’analyse en configuration bistatique est effectuée dans un premier temps pour un objet sphérique pour lequel une théorie analytique a déjà été développée. Les tracés des diagrammes angulaires expérimentaux et théoriques (à plusieurs fréquences données) montrent l’existence d’
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14

Littlefair, Bryn. "A tribo-dynamic solution for the flexible piston skirt and liner conjunction." Thesis, Loughborough University, 2013. https://dspace.lboro.ac.uk/2134/14900.

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The internal combustion engine is still at the heart of the vast majority of vehicles manufactured worldwide today. For these applications reciprocating pistons are typically employed to convert the pressures generated by internal combustion into mechanical work required by the vehicle. Of the energy supplied to the engine as a whole approximately 17% is lost by means of mechanical friction. The piston ring - liner and piston skirt - liner conjunctions contribute approximately 30% of the overall friction losses in almost equal proportions. It is, therefore, important to note that reduction in
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15

Georgilis, Evangelos. "Engineering of Thermoresponsive Diblock Elastin-like Polypeptides." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0444.

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La thèse présentée porte sur l’ingénierie de diblocs de polypeptides à base de motifs élastine (ELPs) susceptibles de s’auto-assembler sous forme de nanoparticules après modification chimique de certains résidus. La stratégie inclue le développement de diblocs d’ELPs composés d’un bloc hydrophobe comportant l’isoleucine en position hôte, fusionné à l’extrémité N-terminale avec un bloc contenant des résidus méthionine chimiosélectivement modifiables. Des modifications du groupement thioéther permettent en effet l’hydrophilisation du segment ELP correspondant ainsi que l’introduction de groupeme
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16

Alanazi, Hamdan Noman. "Characterization of Elastin-Like Polypeptides Using Viscometry." Cleveland State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=csu1311026986.

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17

Mitchell, Kelsey Ryan. "Broadband Acoustic Liner Optimization Study Using Novel Poro-elastic Materials." Thesis, Virginia Tech, 2015. http://hdl.handle.net/10919/52947.

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With the continual challenges associated with reducing aircraft engine noise, there is need for acoustic liner configurations that target broadband performance. This thesis experimentally and analytically investigates passive noise control methods to improve broadband frequency attenuation through various acoustic liner designs. The inclusion of acoustic metamaterials within these liners is examined and optimized. The metamaterials studied consist of resonant and non-resonant materials which include porous foams, microperforated plates (MPP), and embedded aluminum masses. Through finite elemen
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18

Bosi, Federico. "Eshelby-like forces in elastic structures: theory, experiments and applications." Doctoral thesis, Università degli studi di Trento, 2014. https://hdl.handle.net/11572/367619.

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The Eshelbian force is the main concept of a celebrated theoretical framework associated with the motion of dislocations and, more in general, defects in solid. Similarly, it is proven that a force driving the configuration of an elastic structure is generated through the motion and release mechanism of flexural and torsional energy. This configurational force, analytically derived through different approaches and experimentally validated, provides counterintuitive but crucial effects in elasticity. In particular, it affects equilibrium paths in systems with variable length and instabilities,
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19

Bosi, Federico. "Eshelby-like forces in elastic structures: theory, experiments and applications." Doctoral thesis, University of Trento, 2014. http://eprints-phd.biblio.unitn.it/1369/1/PhD_thesis_Bosi_Federico.pdf.

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The Eshelbian force is the main concept of a celebrated theoretical framework associated with the motion of dislocations and, more in general, defects in solid. Similarly, it is proven that a force driving the configuration of an elastic structure is generated through the motion and release mechanism of flexural and torsional energy. This configurational force, analytically derived through different approaches and experimentally validated, provides counterintuitive but crucial effects in elasticity. In particular, it affects equilibrium paths in systems with variable length and instabilities,
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20

Zhou, Mingjun. "Elastin-Like Peptide Dendrimers: Design, Synthesis, and Applications." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/101661.

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Elastin like peptides (ELPs)—derived from the protein elastin—are widely used as thermoresponsive components in biomaterials due to their LCST (lower critical solution temperature) behavior at a characteristic transition temperature (Tt). While linear ELPs have been well investigated, few reports focused on branched ELPs. Using lysine (Lys, with an additional side-chain amine) as branching units, ELP dendrimers were synthesized by solid-phase peptide synthesis (SPPS) with up to 155 amino acid residues. A secondary structure change with decreasing ratio of random coil and increasing ratio of β-
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21

Furmanski, Andrew P. "Charged-current Quasi-elastic-like neutrino interactions at the T2K experiment." Thesis, University of Warwick, 2015. http://wrap.warwick.ac.uk/74036/.

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T2K is a long-baseline neutrino oscillation experiment based in Japan. The experiment has already measured the appearance of ⌫e in a ⌫μ beam, and is hoping to measure the appearance of ⌫¯e in a ⌫¯μ beam, which would open the possibility of observing CP-violation in the lepton sector. The charged current quasi-elastic (CCQE) interaction (vμ + n -> μ− + p) is of great importance to T2K as it is expected to make up over 80% of the interactions at the oscillation peak (600 MeV). In recent years it has become clear that the most common model describing CCQE interactions on nuclei, the Relativistic
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22

Misseroni, Diego. "Experimental models of Elastic Structures: Tensile Buckling and Eshelby-like Forces." Doctoral thesis, Università degli studi di Trento, 2013. https://hdl.handle.net/11572/367912.

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Mechanical models have been invented, designed and realized to experimentally confirm unexpected behaviours theoretically predicted in elasticity: - instabilities and bifurcations occurring in structures under ‘tensile dead load’ and the influence of the constraint’s curvature; - the presence of an ‘Eshelby-like’ or ‘configurational’ force in structures with movable constraints. Furthermore, ‘classical’ features in elasticity have been substantied by testing small-scale models and observing: - linearity in the mechanical response of a truss-structure, confirming the usual ass
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23

Misseroni, Diego. "Experimental models of Elastic Structures: Tensile Buckling and Eshelby-like Forces." Doctoral thesis, University of Trento, 2013. http://eprints-phd.biblio.unitn.it/1120/1/thesis_misseroni.pdf.

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Mechanical models have been invented, designed and realized to experimentally confirm unexpected behaviours theoretically predicted in elasticity: - instabilities and bifurcations occurring in structures under ‘tensile dead load’ and the influence of the constraint’s curvature; - the presence of an ‘Eshelby-like’ or ‘configurational’ force in structures with movable constraints. Furthermore, ‘classical’ features in elasticity have been substantied by testing small-scale models and observing: - linearity in the mechanical response of a truss-structure, confirming the usual assumption of lin
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24

Campbell, C. P. "Positron collisions with helium and alkaline earth-like atoms." Thesis, Queen's University Belfast, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268223.

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25

Trejo, Miguel. "Theoretical studies of fluid and elastic membranes." Paris 6, 2009. http://www.theses.fr/2009PA066698.

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Cette thèse est consacrée à l'étude théorique des membranes fluides, comme les vésicules lipidiques, et des membranes élastiques comme les tissus vivants. Nous avons utilisé des méthodes alliant calcul variationnel et géométrie différentielle des surfaces pour décrire les divers aspects mécaniques et topologiques de ces objets et leur influence sur leur comportement physique et biologique. Dans une première partie nous avons abordé le problème de la tension de ligne entre deux domaines qui composent une vésicule inhomogène. A partir d'un modèle modifiant la description habituelle de ces membra
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26

Kambow, Sumit H. "Characterization of Elastin-like Polypeptide Micelles Using Capillary Viscometry." Cleveland State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=csu1337605892.

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27

Pascoe, Katie Clare, and n/a. "Heritable and early life growth factors affect arterial elastic tissue defect formation." University of Otago. Dunedin School of Medicine, 2006. http://adt.otago.ac.nz./public/adt-NZDU20070306.160709.

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A German pathologist first described defects in the elastic tissues of human arteries over one hundred years ago. Much evidence now supports the involvement of these elastic tissue defects (ETDs) in the initiation and progression of atherosclerosis, although this association is not well accepted. Recent research has determined that the migration of medial smooth muscle cells into the intima (and therefore the start of the atherosclerotic process) is initiated in an attempt to repair these defects and in addition, that there is a correlation between the extent of intimal thickening and the degr
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28

Rahemi, Hossein. "Thermal fracture service life analysis of a case bonded visco-elastic cylinder." Diss., This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-06062008-172155/.

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29

Akturk, Ozgur. "Assessment Of Tunnel Induced Deformation Field Through 3-dimensional Numerical Models (necatibey Subway Station, Ankara, Turkey)." Phd thesis, METU, 2010. http://etd.lib.metu.edu.tr/upload/12612539/index.pdf.

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In heavily settled areas, deformations induced by the tunnel excavation may cause serious damage to nearby structures. In this study it is aimed to model ground deformations induced by main tunnels and connection tunnels excavations as well as groundwater drainage. Therefore, it is necessary to study effective means of controlling tunnel induced deformations. The main parameters affecting the failure and deformation state of the soil around a circular underground opening are the physical characteristics of the soil, the diameter of the opening, and the support pressure. During the construction
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30

Li, Zhen. "Investigation of Tribolium castaneum resilin, a rubber-like insect cuticular protein." Thesis, Kansas State University, 2013. http://hdl.handle.net/2097/16287.

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Master of Science<br>Department of Biochemistry<br>Michael R. Kanost<br>Resilin is a rubber-like cuticular protein found in many insect species. Resilin is important for jumping and flying of those insects due to the properties of high elasticity and efficient energy storage. Some recombinant proteins or peptides derived from resilin sequences have been synthesized to produce biomaterials that mimic the remarkable properties of resilin. This research focused on resilin in the red flour beetle, Tribolium castaneum. A cDNA for T. castaneum resilin was inserted into plasmid vectors for expression
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31

Leonard, Alex. "Elastin Like Polypeptides as Drug Delivery Vehicles in Regenerative Medicine Applications." Scholar Commons, 2016. http://scholarcommons.usf.edu/etd/5981.

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Elastin like polypeptides (ELPs) are a class of naturally derived biomaterials that are non-immunogenic, genetically encodable, and biocompatible making them ideal for a variety of biomedical applications, ranging from drug delivery to tissue engineering. Also, ELPs undergo temperature-mediated inverse phase transitioning, which allows them to be purified in a relatively simple manner from bacterial expression hosts. Being able to genetically encode ELPs allows for the incorporation of bioactive peptides and functionalization of ELPs. This work utilizes ELPs for regenerative medicine and drug
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32

Xiao, Ye. "Synthesis and self-assembly of polysaccharide-b-elastin-like polypeptide bioconjugates." Thesis, Bordeaux, 2019. http://www.theses.fr/2019BORD0172.

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L’association de polysaccharides naturels et de polypeptides recombinants de type élastine (ELP) dans des structures de copolymères à blocs doit permettre d’accéder à des matériaux possédant des propriétés d’auto-assemblage sous l’action de stimuli et potentiellement bioactifs. Nous avons réalisé et présentons ici la synthèse d'une série de bioconjugués polysaccharide-b-ELP, dans lesquels 4 polysaccharides hydrophiles différents ont été couplés à l'extrémité N-terminale de l’ELP par "chimie click".Ces bioconjugués ont été caractérisés par RMN 1D et 2D, SEC et FTIR. Leur thermo-sensibilité et l
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33

Ghoorchian, Ali. "Modification of Behavior of Elastin-like Polypeptides by Changing Molecular Architecture." Cleveland State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=csu1336745204.

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34

Petitdemange, Rosine. "Chemoselective modifications of recombinant elastin-like polypeptides : tuning thermosensitivity and bioactivity." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0360/document.

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La thèse présentée porte sur la préparation de dérivés de polypeptides recombinant à base de motifs élastine (ELPs) ainsi que sur l'étude de leurs propriétés physicochimiques et biologiques. Des ELPs contenant des résidus méthionine ont été modifiés de manière chimiosélective soit en utilisant des halogénures d'alkyle ou différents époxydes, soit par oxydation des résidus méthionine. La caractérisation par RMN et par spectrométrie de masse des composés obtenus a permis de confirmer leur fonctionnalisation quantitative. L'étude de la réponse en température de ces dérivés d'ELP par des mesures d
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35

Tang, Mingjie. "SYNTHESIS AND CHARACTERIZATION OF pH-RESPONSIVE ELASTIN-LIKE POLYPEPTIDES WITH DIFFERENT CONFIGURATIONS." Cleveland State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=csu1376784321.

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36

Helm, Eric. "Solute Partitioning in Elastin-like Polypeptides: A Foundation for Drug Delivery Applications." Cleveland State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=csu1450790146.

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37

WAN, JIA HONG. "Investigation into the phase separation behavior of concentrated elastin-like polypeptide solutions." Cleveland State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=csu1471923273.

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38

Ravanbakhsh, Samyar. "Elastic travel demand analysis - An application to the West link railway in Gothenburg." Thesis, Linköpings universitet, Kommunikations- och transportsystem, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-126542.

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Today the regional railway system lacks the capacity at the central station in order to meet the demand for both the public and freight transportation. The West link is a railway infrastructure project that is expected to both increase train commuting and also increase the train capacity at the central station in Gothenburg. The purpose of this thesis work is to do an elastic demand analysis between car traffic and the West Link in modal shift to determine how many travelers will change mode. Also traffic simulations will be made to investigate the traffic conditions around the stations when t
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39

Ju, Min. "Optimisation de la protection des réseaux optiques de nouvelle génération." Thesis, Avignon, 2018. http://www.theses.fr/2018AVIG0226/document.

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La tolérance aux pannes est une propriété très importante des réseaux optiques de nouvelle génération. Cette thèse aborde la conception des mécanismes de protection contre des pannes liées à la défaillance d’une fibre optique ou à une catastrophe naturelle. Deux systèmes de protection classiques, à savoir la protection par des cycles préconfigurés(p-cycles) et la protection du chemin de secours, sont étudiés pour atteindre une efficacité de protection élevée, tout en considérant le coût de l’équipement optique,la consommation d’énergie et l’utilisation de la ressource spectrale. Ces problèmes
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Wheeler, Charles M. (Charles Michael). "A scalable process for rapid and uniform assembly of repeated origami-like structures using elastic membranes." Thesis, Massachusetts Institute of Technology, 2015. http://hdl.handle.net/1721.1/100307.

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Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2015.<br>This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.<br>Cataloged from student-submitted PDF version of thesis.<br>Includes bibliographical references (pages 88-90).<br>The purpose of this work is the modeling and prototyping of a highly parallelized process capable of folding large and compliant two-dimensional sheets into ordered, three-dimensional structures. The direct application of this work is th
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Cole, James T. "The Synthesis and Characterization of Novel Elastin-Like Polypeptides Containing an Oligomerization Domain." Cleveland State University / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=csu1244042529.

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42

Otto, Kevin Michael. "Characterization of Highly Concentrated Elastin-like Polypeptide Solutions:Rheometric Properties and Phase Separation Analysis." Cleveland State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=csu1431697338.

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43

Kwansa, Albert Lawrence. "Molecular Dynamics and Mechanical Behavior of Collagen Type I and its Lysine/Hydroxylysine-derived Crosslinks." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/50933.

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Collagen type I is an extracellular matrix (ECM) protein that affords tensile strength and biological scaffolding to numerous vertebrate and invertebrate tissues. This strength has been attributed to the triple-helical structure of the collagen type I molecules, their organization into fibrils, and the presence of inter-molecular, covalent, enzymatic crosslinks. There are several different types of these crosslinks; their composition is tissue-specific and dependent upon factors such as age and health. Furthermore, these enzymatic crosslinks tend to form specifically at amino/N- and carboxy/C-
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44

OH, JOONGSEOK. "DESIGN OF RECOMBINANT TENEBRIO MOLITOR ANTIFREEZE PROTEIN FOR PURIFICATION USING ELASTIN-LIKE POLYPEPTIDE TAG." Cleveland State University / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=csu1409230499.

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45

Cole, James T. "The Synthesis and Characterization of Multifunctional Nanoparticles of Elastin-Like Polypeptides for Theranostic Applications." Cleveland State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=csu1461674813.

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46

Pokorný, Pavel. "Odhad zbytkové životnosti železničního dvojkolí." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2012. http://www.nusl.cz/ntk/nusl-230407.

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The first part of this master's thesis deals with the high cycle fatigue of materials, especially on growing cracks using linear elastic fracture mechanics. Much of this work is focused on the concept of stress intensity factor. This concept is nowadays one of the most widely used concepts for describing a body with crack. The first part ends with theoretical approaches to determine the residual fatigue life of the body with a crack. The second part of this master's thesis is focused on the determination of residual fatigue life of a specified railway wheelset. An existence of crack-like defec
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47

Harrington, Christine. "The Prevalence of Pseudoxanthoma Elasticum-like Connective Tissue Changes in an Oral Biopsy Service." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1308062343.

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48

Rocadenbosch, Burillo Francesc. "Lidar sensing of the atmosphere: receiver design and inversion algorithms for an elastic system." Doctoral thesis, Universitat Politècnica de Catalunya, 1996. http://hdl.handle.net/10803/6909.

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LIDAR es un acrónimo de LIght Detection And Ranging. En la presente tesis, se usan técnicas basadas en lidar elástico para monitorizar la atmósfera remotamente y derivar información cuantitativa acerca de sus parámetros ópticos. Esta tesis doctoral comprende el diseño y operación de una estación lidar elástica basada en un láser pulsado de Nd:YAG operando a las longitudes de onda de 1064 y 532 nm, en lo que se refiere a los sistemas de recepción, control y diseño de algoritmos de inversión. Básicamente, puede dividirse en tres partes bien diferenciadas: La primera (Caps. 1, 2 y 3) comprende e
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Amirova, Svetlana R. "The influence of a simple shear deformation on a long wave motion in a pre-stressed incompressible elastic layer." Thesis, Keele University, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491831.

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50

Johnson, Tamina L. "Elastin-Like Polypeptide Fusion Tag as a Protein-Dependent Solubility Enhancer of Cysteine-Knot Growth Factors." Scholar Commons, 2018. https://scholarcommons.usf.edu/etd/7629.

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Abstract:
Elastin-like peptide (ELP) fusions promote therapeutic delivery and efficacy. Recombinant proteins, like neurotrophins, lack bioavailability, have short in vivo half-lives, and require high manufacturing costs. Fusing recombinant proteins with genetically encodable ELPs will increase bioavailability, enhance in vivo solubilization, as well as provide a cost-effective method for purification without the need for chromatography. During expression of neurotrophin-ELP (N-ELP) fusions, dense water-insoluble aggregates known as inclusion bodies (IBs) are formed. Inclusion bodies are partially and mi
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