To see the other types of publications on this topic, follow the link: Elastic liposome.
Journal articles on the topic 'Elastic liposome'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Elastic liposome.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Hattori, Yoshiyuki, Masataka Date, Shohei Arai, Kumi Kawano, Etsuo Yonemochi, and Yoshie Maitani. "Transdermal Delivery of Small Interfering RNA with Elastic Cationic Liposomes in Mice." Journal of Pharmaceutics 2013 (December 26, 2013): 1–6. http://dx.doi.org/10.1155/2013/149695.
Full textAbstract:
We developed elastic cationic liposomal vectors for transdermal siRNA delivery. These liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) as a cationic lipid and sodium cholate (NaChol) or Tween 80 as an edge activator. When NaChol or Tween 80 was included at 5, 10, and 15% (w/w) into DOTAP liposomal formulations (C5-, C10-, and C15-liposomes and T5-, T10-, and T15-liposomes), C15- and T10-liposomes showed 2.4- and 2.7-fold-higher elasticities than DOTAP liposome, respectively. Although the sizes of all elastic liposomes prepared in this study were about 80–90 nm, the sizes of C5-, C10- and C15-liposome/siRNA complexes (lipoplexes) were about 1,700–1,800 nm, and those of T5-, T10-, and T15-lipoplexes were about 550–780 nm. Their elastic lipoplexes showed strong gene suppression by siRNA without cytotoxicity when transfected into human cervical carcinoma SiHa cells. Following skin application of the fluorescence-labeled lipoplexes in mice, among the elastic lipoplexes, C15- and T5-lipoplexes showed effective penetration of siRNA into skin, compared with DOTAP lipoplex and free siRNA solution. These data suggest that elastic cationic liposomes containing an appropriate amount of NaChol or Tween 80 as an edge activator could deliver siRNA transdermally.
2
Chaurasiya, Priyam, Ritesh Agarwal, and Kavita R. Loksh. "Development and Characterization of Elastic Liposomes of Metronidazole for the Treatment of Bacterial Infection." Journal of Drug Delivery and Therapeutics 10, no. 6-s (2020): 83–88. http://dx.doi.org/10.22270/jddt.v10i6-s.4454.
Full textAbstract:
Objective: The objective of present study is to develop and evaluate the elastic liposomes of metronidazole so as to provide the sustained release and improve its bioavailability.
 Methods: Elastic liposomes were prepared by rotary evaporation method using Span 80 and Span 60 as a surfactants. The prepared elastic liposomes were evaluated for entrapment efficiency, vesicle size, In vitro drug release.
 Results: The drug release profiles from different elastic liposomes-in-vehicle formulations were in agreement with the physicochemical properties of the formulations. The formulation prepared showed an average vesicle size 185.4nm. The amount of drug entrapped into the elastic liposomes formulations was determined. The entrapment efficiency was found to be 73.45±0.78 %. A good amount of drug was entrapped in the liposome formulations prepared. Based on different parameters formulations of batch TG2 was found to be the best formulations. Stability study was performed on the selected formulation TG2. When the regression coefficient values of were compared, it was observed that ‘r’ values of first order was maximum i.e. 0.993 hence indicating drug release from formulations was found to follow Korsmeyer Peppas model release kinetics
 Conclusion: These results indicate that elastic liposome can function as probable drug delivery systems to enhance transdermal permeation of metronidazole for treating the topical infections.
 Keywords: Metronidazole, Elastic liposomes, Topical administration, Skin infection
3
Hou, Xueqin, Xinyu Qiu, Yaping Wang, Shuangshuang Song, Yifan Cong, and Jifu Hao. "Application and Efficacy of Melatonin Elastic Liposomes in Photoaging Mice." Oxidative Medicine and Cellular Longevity 2022 (March 8, 2022): 1–15. http://dx.doi.org/10.1155/2022/7135125.
Full textAbstract:
Transdermal drug delivery system is a preferable choice to overcome the low bioavailability of oral medication. Elastic liposomes have shown great effectiveness for percutaneous transport of melatonin (MLT). In this study, the elastic liposomes loaded with MLT were prepared using thin-film dispersion method and optimized through the central composite design (CCD) approach. The physicochemical properties and skin permeation against UV-induced skin photoaging efficacy of the developed MLT-ELs were assessed. The average size of the MLT-ELs was about 49 nm with a spherical shape and high encapsulation efficiency (73.91%) and drug loading (9.92%). The results of FTIR, DSC, and XRD revealed that the chemical structure of MLT was not changed after prepared elastic liposomes, and the drug was successfully encapsulated in the elastic liposome membrane material. In vitro skin permeation evaluation showed that the cumulative penetration of elastic liposomes was 1.5 times higher than that of conventional liposomes, highlighting that the elastic liposomes more easily penetrated into the body. The photoaging experiment results indicated that topical MLT-EL treatment ameliorated the skin elasticity, enhanced the skin hydration level, and preserved the integrity of dermal collagen and elastic fibers. It could be concluded that the elastic liposomes might serve as a promising platform for the transdermal delivery of melatonin.
4
Pamplona, D., and M. H. Santana. "Tensile Instability of Liposomes in the Presence of Surfactants." Applied Mechanics Reviews 50, no. 11S (1997): S162—S167. http://dx.doi.org/10.1115/1.3101829.
Full textAbstract:
Light scattering and electron microscopy have been utilized in some research in order to study the transition induced by adding surfactant to the aqueous solution of small liposome vesicles. Using the theory of elastic membranes to describe the mixed bilayer of the liposome, an extensive deformation analysis of the enlarged vesicle is reported in the present work. An investigation of pressure versus radius reveals the existence of stable and unstable configurations of equilibrium. From obeservation of the Total Potential Energy function, the instability is recognized and discussed in detail. Besides its value in explaining some very interesting phenomena observed in the experimental study of liposomes in the presence of surfactant, the analysis presented in this paper can be useful in pointing out a way to predict the surfactant critical concentration when the liposome breaks into micelles. This rupture is an undesired phenomenon since the liposomes can be used to transport drugs.
5
Pamplona, D. "Transformation of Liposomes: Mechanical Behavior and Stability." Applied Mechanics Reviews 46, no. 11S (1993): S289—S294. http://dx.doi.org/10.1115/1.3122647.
Full textAbstract:
Liposomes are small artificial vesicles of lipid bilayer, wich enclose and are surrounded by water. Morphological transformations in liposomes, starting from a spherical shape, due to changes in the osmotic pressure, have been described in the literature. The first transformation is into a circular biconcave form, afterwards the biconcave side view is maintained, while the front view reveals transformations into elliptical or regular polygonal forms, usually triangular, square or pentagonal. Finite elasticity and the theory of thin shells were used to analyse the behavior of the liposomes under decreasing volume. The biological membrane was considered as a two dimensional fluid layer, exhibiting solid properties to some extent, e.g., elasticity. The stability of the liposmes was studied by using the method of elastic perturbation to obtain the critical pressure for the biconcave transformation and the long liposome tubes. The transformations to elliptical and regular polygonal forms were studied using the linear stability equations of elasticity.
6
Chaudhari, Ramesh, Nikunj Tandel, Kiran Sahu, et al. "Transdermal Immunization of Elastic Liposome-Laden Recombinant Chimeric Fusion Protein of P. falciparum (PfMSP-Fu24) Mounts Protective Immune Response." Nanomaterials 11, no. 2 (2021): 406. http://dx.doi.org/10.3390/nano11020406.
Full textAbstract:
Transdermal immunization exhibits poor immunogenic responses due to poor permeability of antigens through the skin. Elastic liposomes, the ultradeformable nanoscale lipid vesicles, overcome the permeability issues and prove a versatile nanocarrier for transcutaneous delivery of protein, peptide, and nucleic acid antigens. Elastic liposome-mediated subcutaneous delivery of chimeric fusion protein (PfMSP-Fu24) of Plasmodium falciparum exhibited improved immunogenic responses. Elastic liposomes-mediated immunization of PfMSP-Fu24 conferred immunity to the asexual blood-stage infection. Present study is an attempt to compare the protective immune response mounted by the PfMSP-Fu24 upon administered through transdermal and intramuscular routes. Humoral and cell-mediated immune (CMI) response elicited by topical and intramuscularly administered PfMSP-Fu24-laden elastic liposomes (EL-PfMSP-Fu24) were compared and normalized with the vehicle control. Sizeable immune responses were seen with the transcutaneously immunized EL-PfMSP-Fu24 and compared with those elicited with intramuscularly administered antigen. Our results show significant IgG isotype subclass (IgG1and IgG3) response of specific antibody levels as well as cell-mediated immunity (CMI) activating factor (IFN-γ), a crucial player in conferring resistance to blood-stage malaria in mice receiving EL-PfMSP-Fu24 through transdermal route as compared to the intramuscularly administered formulation. Heightened immune response obtained by the vaccination of EL-PfMSP-Fu24 was complemented by the quantification of the transcript (mRNA) levels cell-mediated (IFN-γ, IL-4), and regulatory immune response (IL-10) in the lymph nodes and spleen. Collectively, elastic liposomes prove their immune-adjuvant property as they evoke sizeable and perdurable immune response against PfMSP-Fu24 and justify its potential for the improved vaccine delivery to inducing both humoral and CM immune response.
7
Kasetvetin, Chawankom, Soravoot Rujiviphat, and Waree Tiyaboonchai. "DEVELOPMENT OF HYALURONIC ACID LOADED ELASTIC LIPOSOME." Thai Journal of Pharmaceutical Sciences 38 (January 1, 2013): 152–55. http://dx.doi.org/10.56808/3027-7922.2032.
Full text
8
Lim, Myoung-Sun, Seat-Byeol Han, Soon-Sik Kwon, Min-A. Park, and Soo-Nam Park. "Elastic Liposome Formulation for Transdermal Delivery of Rutin." Journal of the Society of Cosmetic Scientists of Korea 38, no. 2 (2012): 147–54. http://dx.doi.org/10.15230/scsk.2012.38.2.147.
Full text
9
Winnik, Françoise M., Alexander Adronov, and Hiromi Kitano. "Pyrene-labeled amphiphilic poly-(N-isopropylacrylamides) prepared by using a lipophilic radical initiator: synthesis, solution properties in water, and interactions with liposomes." Canadian Journal of Chemistry 73, no. 11 (1995): 2030–40. http://dx.doi.org/10.1139/v95-251.
Full textAbstract:
Fluorescently labeled amphiphilic poly-(N-isopropylacrylamides) (PNIPAM) substituted with a N-[4-(1-pyrenyl)butyl]-N-n-octadecyl group at the chain end were prepared by free-radical polymerization in dioxane of N-isopropylacrylamide (NIPAM) using 4,4′-azobis{4-cyano-N,N-[4-(1-pyrenyl)butyl]-n-octadecyl}pentanamide as the initiator. The solution properties of the polymers in water were studied as a function of polymer concentration and temperature. Quasi-elastic light-scattering measurements and fluorescence experiments monitoring the pyrene excimer and pyrene monomer emissions revealed the presence of multimolecular polymeric micelles below the lower critical solution temperature (LCST) of PNIPAM. These underwent partial, reversible reorganization as they were heated above the LCST. The interactions of the pyrene-labeled amphiphilic PNIPAM with dimyristoylphosphatidylcholine (DMPC) liposomes have been examined in water at 25 °C. From fluorescence experiments it was established that the polymeric micelles are disrupted irreversibly upon contact with the liposomes. The anchoring of the polymer chains occurs by insertion of their hydrophobic tail within the phospholipidic bilayer, as evidenced from a large decrease of the pyrene excimer emission relative to pyrene monomer emission. The copolymers remained anchored within the bilayer as the temperature of the copolymer–liposome suspension was raised above the LCST of PNIPAM. Keywords: liposome, poly-(N-isopropylacrylamide), fluorescence, micelles.
10
Hupfeld, Stefan, Ann Mari Holsæter, Merete Skar, Christer B. Frantzen, and Martin Brandl. "Liposome Size Analysis by Dynamic/Static Light Scattering upon Size Exclusion-/Field Flow-Fractionation." Journal of Nanoscience and Nanotechnology 6, no. 9 (2006): 3025–31. http://dx.doi.org/10.1166/jnn.2006.454.
Full textAbstract:
The aim of the current study was to analyse the particle size distribution of a liposome dispersion, which contained small egg phosphatidylcholine vesicles and had been prepared by high-pressure homogenisation, by various size analysis techniques. Such liposomes were chosen since they can be looked at as a prototype of drug nano-carriers. Three sub-micron particle size analysis techniques were employed: (1) fixed-angle quasi-elastic laser light scattering or photon correlation spectroscopy (PCS), (2) size exclusion chromatographic (SEC) fractionation with subsequent (off-line) PCS size-analysis and quantification of the amount of particles present in the sub-fractions, and (3) field-flow-fractionation coupled on-line with a static light scattering and a refractive index (RI)-detector. When designing liposome-based drug carrier systems, a reliable and reproducible analysis of their size and size distribution is of paramount importance: Not only does liposome size influence the nanocarrier's in-vitro characteristics such as drug loading capacity, aggregation and sedimentation but also it is generally acknowledged that the pharmacokinetic behaviour and biodistribution of the carrier is strongly size-dependent. All three approaches of liposome size analysis used here were found to yield useful results, although they were not fully congruent. PCS indicated either a broad, mono-modal, log-normal size distribution in the range of below 20 to over 200 nm in diameter, or alternatively, a bimodal distribution with two discrete peaks at 30 to 70 nm and 100 to over 200 nm. Which of the two distribution models represented the best fit depended primarily on the data collection times used. In contrast, both fractionating techniques revealed a size distribution with a large, narrow peak well below 50 nm and a minor, broad, overlapping peak or tail extending to over 100 nm in diameter. The observed differences in liposome size distribution may be explained by the inherent limitations of the different size analysis techniques, such as the detection limit and the fact that PCS is overemphasizing bigger particle sizes.
11
Mendová, Katarína, Martin Otáhal, Mitja Drab, and Matej Daniel. "Size Matters: Rethinking Hertz Model Interpretation for Cell Mechanics Using AFM." International Journal of Molecular Sciences 25, no. 13 (2024): 7186. http://dx.doi.org/10.3390/ijms25137186.
Full textAbstract:
Cell mechanics are a biophysical indicator of cell state, such as cancer metastasis, leukocyte activation, and cell cycle progression. Atomic force microscopy (AFM) is a widely used technique to measure cell mechanics, where the Young modulus of a cell is usually derived from the Hertz contact model. However, the Hertz model assumes that the cell is an elastic, isotropic, and homogeneous material and that the indentation is small compared to the cell size. These assumptions neglect the effects of the cytoskeleton, cell size and shape, and cell environment on cell deformation. In this study, we investigated the influence of cell size on the estimated Young’s modulus using liposomes as cell models. Liposomes were prepared with different sizes and filled with phosphate buffered saline (PBS) or hyaluronic acid (HA) to mimic the cytoplasm. AFM was used to obtain the force indentation curves and fit them to the Hertz model. We found that the larger the liposome, the lower the estimated Young’s modulus for both PBS-filled and HA-filled liposomes. This suggests that the Young modulus obtained from the Hertz model is not only a property of the cell material but also depends on the cell dimensions. Therefore, when comparing or interpreting cell mechanics using the Hertz model, it is essential to account for cell size.
12
Prima, Giulia Di, Fabio Librizzi, and Rita Carrotta. "Light Scattering as an Easy Tool to Measure Vesicles Weight Concentration." Membranes 10, no. 9 (2020): 222. http://dx.doi.org/10.3390/membranes10090222.
Full textAbstract:
Over the last few decades, liposomes have emerged as promising drug delivery systems and effective membrane models for studying biophysical and biological processes. For all applications, knowing their concentration after preparation is crucial. Thus, the development of methods for easily controlling vesicles concentration would be of great utility. A new assay is presented here, based on a suitable analysis of light scattering intensity from liposome dispersions. The method, tested for extrusion preparations, is precise, easy, fast, non-destructive and uses a tiny amount of sample. Furthermore, the scattering intensity can be measured indifferently at different angles, or even by using the elastic band obtained from a standard spectrofluorimeter. To validate the method, the measured concentrations of vesicles of different matrix compositions and sizes, measured by light scattering with different angles and instruments, were compared to the data obtained by the standard Stewart assay. Consistent results were obtained. The light scattering assay is based on the assessment of the mass fraction lost in the preparation, and can be applied for methods such as extrusion, homogenization, French press and other microfluidic procedures.
13
Weinbaum, Sheldon, and Shu Chien. "Lipid Transport Aspects of Atherogenesis." Journal of Biomechanical Engineering 115, no. 4B (1993): 602–10. http://dx.doi.org/10.1115/1.2895547.
Full textAbstract:
In this review we shall examine the current understanding of events that lead to the incipient formation of the early foam cell lesion in atherogenesis and its localization. Particular emphasis will be placed on the intimal transport mechanisms that lead to the growth of extracellular lipid liposomes in the intima, since there is now substantial evidence that this growth is the triggering event in the complex sequence of processes that leads to the recruitment of blood borne monocytes into the sub-endothelial intima and their subsequent conversion to macrophages. The role of the endothelium, intimal proteoglycans and internal elastic lamina (IEL) in modulating the transport of low density lipoproteins (LDL) in the subendothelial space will be analyzed and a new hypothesis for the co-localization of liposome formation, cellular level endothelial leakage and monocyte entry described. The possible modifications of LDL in the lipsomes that facilitate the conversion of monocytes into foam cells is summarized. We also discuss the fluid dynamic aspects of intimal transport and the relationship of fluid shear stress to the localization of cellular level endothelial leakage of LDL. The effect of fluid shear on other endothelial cell functions has been recently reviewed in [1].
14
Krivić, Hannah, Sebastian Himbert, and Maikel C. Rheinstädter. "Perspective on the Application of Erythrocyte Liposome-Based Drug Delivery for Infectious Diseases." Membranes 12, no. 12 (2022): 1226. http://dx.doi.org/10.3390/membranes12121226.
Full textAbstract:
Nanoparticles are explored as drug carriers with the promise for the treatment of diseases to increase the efficacy and also reduce side effects sometimes seen with conventional drugs. To accomplish this goal, drugs are encapsulated in or conjugated to the nanocarriers and selectively delivered to their targets. Potential applications include immunization, the delivery of anti-cancer drugs to tumours, antibiotics to infections, targeting resistant bacteria, and delivery of therapeutic agents to the brain. Despite this great promise and potential, drug delivery systems have yet to be established, mainly due to their limitations in physical instability and rapid clearance by the host’s immune response. Recent interest has been taken in using red blood cells (RBC) as drug carriers due to their naturally long circulation time, flexible structure, and direct access to many target sites. This includes coating of nanoparticles with the membrane of red blood cells, and the fabrication and manipulation of liposomes made of the red blood cells’ cytoplasmic membrane. The properties of these erythrocyte liposomes, such as charge and elastic properties, can be tuned through the incorporation of synthetic lipids to optimize physical properties and the loading efficiency and retention of different drugs. Specificity can be established through the anchorage of antigens and antibodies in the liposomal membrane to achieve targeted delivery. Although still at an early stage, this erythrocyte-based platform shows first promising results in vitro and in animal studies. However, their full potential in terms of increased efficacy and side effect minimization still needs to be explored in vivo.
15
Kwak, Jong Hyeok, Sungho Kim, Hyuk Kyu Pak, et al. "Preparation of Giant Quantum Dot-Liposome Complexes by the Asolectin Lipid and Theoretical Model for Stabilization of Nanoparticle Inside the Liposome." Journal of Biomaterials and Tissue Engineering 12, no. 9 (2022): 1723–31. http://dx.doi.org/10.1166/jbt.2022.3087.
Full textAbstract:
We prepare giant Quantum dot-Liposome Complexes (QLCs). Quantum dots (QDs) incorporated inside liposome above 10 μm. QLCs is made by using the electro-swelling method combined with spin coating techniques. Three types of PC lipids and asolectin lipid are used for QLCs with HDA (hexadecylamine) coated QDs, which ranged from blue- (diameter ~2.1 nm) to red-emission (diameter ~5.0 nm). As expected, (blue- or) green-emission QDs (smaller than) comparable to the thickness of PC lipid bilayer (~4 nm) are successfully formed QLCs, but QDs bigger than that fail to reproduce. This observation is well-consistent with those reported by Gopakumar et al. Surprisingly, all QDs irrespective of their size are, contrary to PC lipids, successfully loaded into asolectin lipid bilayer. In order to understand what makes different behaviors between PC and asolectin lipids on QLC formation, we suggest a theoretical model based on a geometrical assumptions for deformed lipid bilayer surrounding QD. The main advantage of this model is that the critical size Rcr of QD radius can be decided without calculating elastic free energy. As a result, it predicts that only QDs below the critical size (diameter ~3.0 nm) can be loaded in a typical PC-lipid, but all size of QDs can be incorporated into asolectin bilayer under the assumption of two different curvatures on deformed monolayer.
16
Altamimi, Mohammad A., Afzal Hussain, Sultan Alshehri, and Syed Sarim Imam. "Experimental Design Based Optimization and Ex Vivo Permeation of Desmopressin Acetate Loaded Elastic Liposomes Using Rat Skin." Pharmaceutics 13, no. 7 (2021): 1047. http://dx.doi.org/10.3390/pharmaceutics13071047.
Full textAbstract:
The study aimed to develop elastic-liposome-based transdermal delivery of desmopressin acetate for enhanced permeation to control enuresis, central diabetes insipidus, and traumatic injury. Elastic liposomes (ELs)-loaded desmopressin acetate was prepared, optimized, and evaluated for improved transdermal permeation profiles using rat skin. Full factorial design with independent factors (X1 for lipid and X2 for surfactant) at three levels was used against four responses (Y1, Y2, Y3, and Y4) (dependent variables). Formulations were characterized for vesicle size, polydispersity index (PDI), zeta potential, % entrapment efficiency (% EE), in vitro drug release, in vitro hemolysis potential, ex vivo drug permeation and drug deposition (DD), and ex vivo vesicle–skin interaction using scanning electron microscopy studies. The optimized formulation ODEL1 based on desirability function was found to have vesicle size, % EE, % DR, and permeation flux values of 118.7 nm, 78.9%, 75.1%, and 5.3 µg/h·cm2, respectively, which were close to predicted values. In vitro release profiles indicated slow and sustained delivery. Permeation flux values of ODEL1 and ODEL2 were 5.3 and 3.1 µg/h·cm2, respectively, which are 7.5- and 4.4-fold higher as compared to DS (0.71 µg/h·cm2). The obtained flux was relatively higher than the clinical target value of the drug for therapeutic efficacy. Moreover, the DD value of ODEL1 was significantly higher than ODEL2 and DS. Hemocompatibility study confirmed safety concerns. Finally, vesicle–skin interaction corroborated mechanistic views of permeation through rat skin. Conclusively, the transdermal delivery may be a suitable alternative to oral and nasal delivery to treat nocturnal enuresis, central diabetes insipidus, hemophilia A and von Willebrand’s disease, and any traumatic injuries.
17
Tyagi, Rajeev K., Neeraj K. Garg, Rajesh Jadon, et al. "Elastic liposome-mediated transdermal immunization enhanced the immunogenicity of P. falciparum surface antigen, MSP-119." Vaccine 33, no. 36 (2015): 4630–38. http://dx.doi.org/10.1016/j.vaccine.2015.06.054.
Full text
18
Chen, Yan-Yan, Ye-Hui Lu, Chun-Hua Ma, Wei-Wei Tao, Jing-Juan Zhu, and Xu Zhang. "A novel elastic liposome for skin delivery of papain and its application on hypertrophic scar." Biomedicine & Pharmacotherapy 87 (March 2017): 82–91. http://dx.doi.org/10.1016/j.biopha.2016.12.076.
Full text
19
Hussain, Afzal, Abdus Samad, Mohammad Ramzan, Mohd Neyaz Ahsan, Zia Ur Rehman, and Farhan Jalees Ahmad. "Elastic liposome-based gel for topical delivery of 5-fluorouracil: in vitro and in vivo investigation." Drug Delivery 23, no. 4 (2014): 1115–29. http://dx.doi.org/10.3109/10717544.2014.976891.
Full text
20
Li, Lingling, Yangde Zhang, Shiwei Han, et al. "Penetration Enhancement of Lidocaine Hydrochlorid by a Novel Chitosan Coated Elastic Liposome for Transdermal Drug Delivery." Journal of Biomedical Nanotechnology 7, no. 5 (2011): 704–13. http://dx.doi.org/10.1166/jbn.2011.1333.
Full text
21
Hussain, Afzal, Md Wasimul Haque, Sandeep Kumar Singh, and Farhan Jalees Ahmed. "Optimized permeation enhancer for topical delivery of 5-fluorouracil-loaded elastic liposome using Design Expert: part II." Drug Delivery 23, no. 4 (2015): 1242–53. http://dx.doi.org/10.3109/10717544.2015.1124473.
Full text
22
Kim, Sung Tae, Kyung-Mi Lee, Hyun-Joo Park, Su-Eon Jin, Woong Shick Ahn, and Chong-Kook Kim. "Topical delivery of interleukin-13 antisense oligonucleotides with cationic elastic liposome for the treatment of atopic dermatitis." Journal of Gene Medicine 11, no. 1 (2009): 26–37. http://dx.doi.org/10.1002/jgm.1268.
Full text
23
Calladine, C. R., and J. A. Greenwood. "Mechanics of Tether Formation in Liposomes." Journal of Biomechanical Engineering 124, no. 5 (2002): 576–85. http://dx.doi.org/10.1115/1.1500341.
Full textAbstract:
It is well-known that a “tether” may be drawn out from a pressurized liposome by means of a suitably applied radial-outward force applied locally to the lipid bilayer. The tether is a narrow, uniform cylindrical tube, which joins the main vesicle in a short “transition region.” A first-order energy analysis establishes the broad relationship between the force F needed to draw the tether, the radius R0 of the tether, the bending-stiffness constant B for the lipid bilayer and the membrane tension T in the pressurized liposome. The aim of the present paper is to study in detail the “transition region” between the tether and the main vesicle, by means of a careful application of the engineering theory of axisymmetric shell structures. It turns out that the well-known textbook “thin-shell” theory is inadequate for this purpose, because the tether is evidently an example of a thick-walled shell; and a novel ingredient of the present study is the introduction of elastic constitutive relations that are appropriate to the thick-shell situation. The governing equations are set up in dimensionless form, and are solved by means of a “shooting” technique, starting with a single disposable parameter at a point on the meridian in the tether, which can be adjusted until the boundary conditions at the far “equator” of the main vessel are satisfied. It turns out that the “transition region” between the tether and the main vessel is well characterized by only a few parameters, while the tether and main vessel themselves are described by very simple equations. Introduction of the thick-shell constitutive relation makes little difference to the conformation of, and stress-resultants in, the main vessel; but it makes a great deal of difference in the tether itself. Indeed, a kind of phase-change appears to take place in the “transition region” between these two zones of the liposome.
24
Jiang, Wanhang, Sara Toufouki, Subhan Mahmood, et al. "Development of Liposome-Based Hydrogel Patches Incorporating Essential Oils of African Plants and Deep Eutectic Solvents." Gels 11, no. 5 (2025): 364. https://doi.org/10.3390/gels11050364.
Full textAbstract:
A nanoliposome-integrated polymeric hydrogel was developed for the controlled release of essential oils (Argania spinosa, Passiflora edulis). A deep eutectic solvent (DES) composed of betaine and phytic acid enhanced the solubility and stability of essential oils, facilitating uniform encapsulation within nanoliposomes. The hydrogel exhibited a swelling capacity of 100% and retained 51.7% of water after 7 h, ensuring prolonged hydration. Structural analysis confirmed a homogeneous dispersion of nanoliposomes, contributing to the gradual release of bioactive components. Additionally, the hydrogel demonstrated high mechanical strength (7.5 MPa), ensuring flexibility and durability. The polymeric network, formed by acrylamide, sodium alginate, and bentonite, provided a stable and elastic matrix, optimizing water retention and mechanical performance. The controlled diffusion mechanism of the nanoliposomes was validated through in vitro release studies, indicating Fickian-controlled release behavior. These findings highlight the potential of this polymeric hydrogel system as a functional material for skincare formulations, offering enhanced hydration and sustained bioactive delivery.
25
Pamplona, D. C., and C. R. Calladine. "Aspects of the Mechanics of Lobed Liposomes." Journal of Biomechanical Engineering 118, no. 4 (1996): 482–88. http://dx.doi.org/10.1115/1.2796034.
Full textAbstract:
Hotani has studied, by means of dark-field light microscopy, morphological transformations which unilamellar liposomes undergo when their interior volume decreases steadily with time as a consequence of osmosis. In a previous paper, we made a theoretical study of the initial buckling of an originally spherical vesicle into the observed oblate spheroidal shape; and we argued that some in-plane shear elastic stiffness is required—in addition to the well-known flexural stiffness of the lipid bilayer—in order to explain the observed phenomena. In the present paper, we consider a later stage in the chain of morphological transitions observed by Hotani, when a series of cudgel-shaped lobes have sprung out of a previously axisymmetric, biconcave-shaped vesicle. Specifically, we compare the observed shapes of such lobes with half of a series of “peanut”-shaped vesicles that are an equilibrium conformation of an initially spherical liposome under reduced internal volume. We find that the shapes do not match well. On the other hand, the observed lobe forms do match satisfactorily portions of “undulating tube” shapes which evolve from a hypothetical cylindrical vesicle, according to some simple calculations. In view of this agreement, we are led to propose that the formation of cudgel-shaped lobes requires some sliding of one lipid monolayer over another. This conflicts, of course, with the Love-Kirchhoff hypothesis which is normally invoked at the outset of analyses of lipid vesicles by means of classical thin-shell theory; but it is in accord with previous suggestions in the context of more obviously severe distortion of the lipid bilayer.
26
Wang, Juan, Jia Wang, Mingyue Zheng, and Da Li. "Effects of Different Concentrations of AmB on the Unsaturated Phospholipid–Cholesterol Membrane Using the Langmuir Monolayer and Liposome Models." Molecules 29, no. 23 (2024): 5659. http://dx.doi.org/10.3390/molecules29235659.
Full textAbstract:
Amphotericin B (AmB) causes toxicity to the erythrocyte membrane, leading to hemolysis, which limits the clinically effective dose for AmB intravenous therapy in invasive fungal infections. The molecular mechanism by which AmB adheres to the membrane of erythrocytes is the key factor in causing AmB to be toxic to the membrane of erythrocytes, but it is not yet fully understood; the mechanism by which AmB adheres to the liquid microdomains with higher fluidity formed by cholesterol and unsaturated phospholipids remains especially unclear. This study examined the adsorption of AmB at different concentrations, 5, 45, 85, and 125 μg/mL, on unsaturated phospholipid membranes containing 50 mol% cholesterol. The thermodynamic properties and structure of DOPC monolayers and DOPC/cholesterol mixed monolayers at different concentrations of AmB have been investigated using the Langmuir monolayer model and the BAM method. The impact of varying concentrations of AmB on the hydrophilic and hydrophobic domains of the DOPC bilayers and the DOPC/cholesterol mixed bilayers have also been discussed using large unilamellar vesicle liposomes and fluorescence techniques. It is shown that for AmB concentrations greater than 5 μg/mL, with an increase in AmB’s concentration, the reorganization time for the DOPC/cholesterol monolayer increases, and the elastic modulus of the DOPC/cholesterol mixed monolayer decreases. In particular, when AmB’s concentration is higher than 85 μg/mL, the liquid-condensed phase domains on the DOPC/cholesterol monolayer reduce significantly and the liquid-expanded phase domain enlarges from the BAM images. When the AmB concentration reaches 5 μg/mL, the disorder of the hydrophobic and hydrophilic domains of the DOPC/cholesterol bilayer increases as the AmB concentration increases. The way in which AmB interacts with the DOPC/cholesterol mixed membrane is related to the concentration of AmB. The higher the concentration of AmB, the more likely it is to remove cholesterol from the unsaturated phospholipid membrane. The results are helpful to understand the mechanism of AmB’s toxicity to the erythrocyte’s membrane, which has a guiding value for seeking ways to reduce the AmB’s toxicity.
27
UCHIYAMA, Yoshiko, Hiroharu YUI, and Tsuguo SAWADA. "Adsorption and Desorption Behaviors of Cationic Liposome-DNA Complexes upon Lipofection in Inside and Outside Biomembrane Models Using a Dynamic Quasi-Elastic Laser Scattering Method." Analytical Sciences 20, no. 11 (2004): 1537–42. http://dx.doi.org/10.2116/analsci.20.1537.
Full text
28
Khan, Rubeena, Prateek Kumar Jain, Basant Khare, et al. "Formulation and Characterization of Novel Transfersomes Gel for Enhance TDDS of Losartan Potassium." Journal of Drug Delivery and Therapeutics 12, no. 4-S (2022): 96–100. http://dx.doi.org/10.22270/jddt.v12i4-s.5525.
Full textAbstract:
A transferosome is the first generation of an elastic liposome prepared from phospholipids and edge activators. An edge activator is often a single-chain surfactant with a high radius of curvature that destabilizes the lipid bilayers of vesicles and increases the deformability of the bilayers, thereby making the vehicle ultra-deformable. Losartan potassium is an orally active angiotensin-II receptor antagonist used in the treatment of hypertension due to mainly blockade of AT1 receptor. It is freely soluble in water, slightly soluble in acetonitrile, and soluble in isopropyl alcohol. The aim of the present study was to investigate the potential of transfersomal gel formulations for transdermal delivery of losartan potassium by reverse phase evaporation method. Characterization of transfersomes gel performed by vesicle size, pH measurements, drug content, entrapment efficiency, in vitro drug diffusion study, spreadability and stability study. In the formulations pH is found to be around 6.8 to 6.9, pH is found in the range of 6 which is compatible with skin. In the formulations spreadability is found to be around 6.75 to 10.11 g m cm/sec. The prepared gel containing losartan potassium-loaded transfersomal formulation was optimized and can be use for topical preparation. The results were obtained which showed that transfersomal gel was a promising candidate for transdermal delivery with targeted and prolonged release of a drug. It also enhances skin permeation of many drugs.
 Keywords: Transferosome gel, Losartan potassium, Antihypertensives, Reverse phase evaporation method
29
Dymek, Michał, Karolina Olechowska, Katarzyna Hąc-Wydro, and Elżbieta Sikora. "Liposomes as Carriers of GHK-Cu Tripeptide for Cosmetic Application." Pharmaceutics 15, no. 10 (2023): 2485. http://dx.doi.org/10.3390/pharmaceutics15102485.
Full textAbstract:
Liposomes are self-assembled spherical systems composed of amphiphilic phospholipids. They can be used as carriers of both hydrophobic and hydrophilic substances, such as the anti-aging and wound-healing copper-binding peptide, GHK-Cu (glycyl-L-histidyl-L-lysine). Anionic (AL) and cationic (CL) hydrogenated lecithin-based liposomes were obtained as GHK-Cu skin delivery systems using the thin-film hydration method combined with freeze–thaw cycles and the extrusion process. The influence of total lipid content, lipid composition and GHK-Cu concentration on the physicochemical properties of liposomes was studied. The lipid bilayer fluidity and the peptide encapsulation efficiency (EE) were also determined. Moreover, in vitro assays of tyrosinase and elastase inhibition were performed. Stable GHK-Cu-loaded liposome systems of small sizes (approx. 100 nm) were obtained. The bilayer fluidity was higher in the case of cationic liposomes. As the best carriers, 25 mg/cm3 CL and AL hydrated with 0.5 mg/cm3 GHK-Cu were selected with EE of 31.7 ± 0.9% and 20.0 ± 2.8%, respectively. The obtained results confirmed that the liposomes can be used as carriers for biomimetic peptides such as copper-binding peptide and that the GHK-Cu did not significantly affect the tyrosinase activity but led to 48.90 ± 2.50% elastase inhibition, thus reducing the rate of elastin degeneration and supporting the structural integrity of the skin.
30
Vidal, Constanza, Johana Lopez-Polo, and Fernando A. Osorio. "Physical Properties of Cellulose Derivative-Based Edible Films Elaborated with Liposomes Encapsulating Grape Seed Tannins." Antioxidants 13, no. 8 (2024): 989. http://dx.doi.org/10.3390/antiox13080989.
Full textAbstract:
Combined use of edible films (EF) with nanoencapsulation systems could be an effective alternative for improving the films’ physical properties and maintaining bioactive compounds’ stability. This research work focuses on the combined use of EF of cellulose-derived biopolymers enriched with liposomes that encapsulate grape seed tannins and on the subsequent evaluation of the physical properties and wettability. Tannin-containing liposomal suspensions (TLS) showed 570.8 ± 6.0 nm particle size and 99% encapsulation efficiency. In vitro studies showed that the release of tannins from liposomes was slower than that of free tannins, reaching a maximum release of catechin of 0.13 ± 0.01%, epicatechin of 0.57 ± 0.01%, and gallic acid of 3.90 ± 0.001% over a 144 h period. Adding liposomes to biopolymer matrices resulted in significant decrease (p < 0.05) of density, surface tension, tensile strength, elongation percentage, and elastic modulus in comparison to the control, obtaining films with greater flexibility and lower breaking strength. Incorporating TLS into EF formulations resulted in partially wetting the hydrophobic surface, reducing adhesion and cohesion compared to EF without liposomes. Results indicate that the presence of liposomes improves films’ physical and wettability properties, causing them to extend and not contract when applied to hydrophobic food surfaces.
31
JAIN, NEHA, Ameeta Argal, and Girendra Gautam. "Elastic liposomes mediated transdermal delivery of verapamil hydrochloride." Journal of Drug Delivery and Therapeutics 8, no. 6 (2018): 16–21. http://dx.doi.org/10.22270/jddt.v8i6.2073.
Full textAbstract:
The aim of present investigation was to formulate and characterize elastic liposomes as a delivery system for transdermal delivery of Verapamil hydrochloride, a drug having low oral bioavailability (approx 20%), short biological half-life and extensive first pass metabolism.Verapamil hydrochloride loaded elastic vesicles were prepared by a slightly modified extrusion method using soya phosphatidylcholine and span 80(edge activator). Prepared elastic vesicles were characterized for various parameters such as vesicle shape, vesicle size and size distribution, entrapment efficiency, elasticity measurements, stability studies and in vitro skin permeation studies through excised rat skin (Sprague Dawley) using a locally fabricated Franz diffusion cell. The entrapment efficiency of elastic vesicles was found to be 59.3±3.6%. In vitro skin permeation of verapamil hydrochloride through excised rat skin (Sprague Dawley) revealed that elastic vesicles led to an enhanced transdermal flux (50.2±4.52 mg/cm2/h) of verapamil hydrochloride as compared to liposomes (11.6±2.12mg/cm2/h). Decreased lag time (0.9 h) was also observed in case of elastic liposomes. Our results indicate the feasibility of elastic liposomes for transdermal delivery of verapamil hydrochloride for improved skin permeation.
 Keywords: Transdermal delivery, Elastic liposomes, Verapamil hydrochloride.
32
Rosmiati, Meiti, Dewi Wulantresna, and Rida Emelia. "Transethosomes as Vesicular Drug Delivery : a Modified Form of Ethosomes and Transfersomes." Ranah Research : Journal of Multidisciplinary Research and Development 7, no. 3 (2025): 2230–45. https://doi.org/10.38035/rrj.v7i3.1432.
Full textAbstract:
Liposomal vesicle formulations can be classified into two categories, namely rigid hard vesicles such as liposomes and elastic vesicles such as transferosome. One of the shortcomings of conventional liposomes is their permeation strength in the stratum corneum, so that in later generations liposomes are designed to be able to better overcome these obstacles. Deformable liposomes, also known as transfersomes, are liposomes that contain edge activators/surfactants. The combination of phospolipids with membrane softening agents allow the transfersome to penetrate pores that are five times smaller than their own diameter, even after it passes through small pores. Ethosomes are liposomes with modified ethanol that act as reservoir systems and offer the continuous delivery of drugs to the desired site. The high concentration of ethanol content in the manufacture of ethosome systems makes this system different from other vesicle systems, because the ethanol content will interfere with the double layer of skin lipids and thus increase the ability of vesicles to penetrate into the stratum corneum. Phospholipids in ethosomes serve as vesicular-forming components. Phospholipids are also reported to act synergistically with ethanol to improve drug permeation in ethosome formulations. Transethosomes are ethanol-based lipid vesicular systems resulting from modifications of ethosome and transfersome systems that can increase penetration in the skin. It is a new generation of the ethosome system which developed by increasing the flexibility of vesicles by redistributing edge activators and lipids on the skin. The mechanism of action of transethosomes is a combination of advantages of both transferosome systems and ethosomes.
33
Shukla, Kavita Verma, Rajat Meshram, and Monika Yadav. "Formulation, Development and Evaluation of Transfersomal Gel of Metronidazole." Journal of Drug Delivery and Therapeutics 9, no. 4-A (2019): 642–45. http://dx.doi.org/10.22270/jddt.v9i4-a.3544.
Full textAbstract:
Metronidazole is an antibiotic that is used to treat a wide variety of infections. It works by stopping the growth of certain bacteria and parasites. This antibiotic treats only certain bacterial and parasitic infections. It may also be used with other medications to treat certain stomach/intestinal ulcers caused by bacteria (H. pylori). A transferosome is the first generation of an elastic liposome prepared from phospholipids and edge activators. An edge activator is often a single-chain surfactant with a high radius of curvature that destabilizes the lipid bilayers of vesicles and increases the deformability of the bilayers, thereby making the vehicle ultra-deformable. The aim of the present study was to investigate the potential of transfersomal gel formulations for transdermal delivery of metronidazole and to evaluate the effect of lipid concentration, ethanol concentration, drug concentration and stirrer time. Characterization of transfersomes performed by vesicle size, surface charge, entrapment efficiency and zeta potential. Characterization of transfersome containing gel performed by the measurement of viscosity, drug content, extrudability study, spreadability and in vitro drug diffusion study. It was found that viscosity of prepared gel was 3560cps, % assay was 98.89±0.45, extrudability was 1156g and spreadibility (g.cm/sec) was found that 11.23(g.cm/sec) respectively. In vitro drug release from transfersomes gel was carried out using Franz diffusion cell method and found 85.56% in 12 hr. In first 30 min it was 22.2 % drug release which slightly high. It was due to the release of free drug present in bag after leaching from transfersomes. Drug release from transferosomal gel formulation was found in very sustained and controlled manner. The results were obtained which showed that transfersomal gel was a promising candidate for transdermal delivery with targeted and prolonged release of a drug. It also enhances skin permeation of many drugs. Keywords: Transfersomal gel, Metronidazole, Antibiotic, Characterization, Franz diffusion cell
34
Huang, Y., D. Rumschitzki, S. Chien, and S. Weinbaum. "A Fiber Matrix Model for the Growth of Macromolecular Leakage Spots in the Arterial Intima." Journal of Biomechanical Engineering 116, no. 4 (1994): 430–45. http://dx.doi.org/10.1115/1.2895794.
Full textAbstract:
A new model is presented for the growth of cellular level macromolecular leakage spots in the arterial intima. The theoretical approach differs from the recent study by Yuan et al. [19] in that it directly models and calculates the intimal transport parameters based on Frank and Fogelman’s [22] ultrastructural observations of the extracellular subendothelial proteoglycan matrix that their rapid freeze etching technique preserves (see Addendum). Using a heterogeneous fiber matrix theory, which includes proteoglycan and collagen components, the model predicts that the Darcy permeability Kp and macromolecular diffusivity D of the subendothelial intima is two orders of magnitude larger than the corresponding values measured in the media, and supports the observations in Lark et al. [24] that the proteoglycan structure of the intima differs greatly from that of the media. Numerical results show that convection parallel to the endothelium is a very significant transport mechanism for macromolecules in the intima in a large region of roughly 200 μm diameter surrounding the leaky cleft. The predictions of the new model for the early-time spread of the advancing convective-diffusive front from the leakage spots in the intima are in close agreement with our experimental measurements for the growth of HRP spots in [20]. The regions of high concentration surrounding the leaky cell, however, are much more limited and cover an area that is typically equivalent to 20 cells. This prediction is consistent with the recent measurements of Truskey et al. for LDL spot size in rabbit aorta [21] and the hypothesis advanced in [19] that there is a colocalization of subendothelial liposome growth and cellular level leakage. Finally, comparison of predicted and experimentally-measured average LDL concentration in leakage spots strongly suggests that there is significant local molecular sieving at the interface between the fenestral openings in the internal elastic lamina and the media.
35
Bogdanov, Anita, László Janovák, Jasmina Vraneš, et al. "Liposomal Encapsulation Increases the Efficacy of Azithromycin against Chlamydia trachomatis." Pharmaceutics 14, no. 1 (2021): 36. http://dx.doi.org/10.3390/pharmaceutics14010036.
Full textAbstract:
Chlamydia trachomatis (C. trachomatis) is an obligate intracellular bacterium linked to ocular and urogenital infections with potentially serious sequelae, including blindness and infertility. First-line antibiotics, such as azithromycin (AZT) and doxycycline, are effective, but treatment failures have also been reported. Encapsulation of antibiotics in liposomes is considered an effective approach for improving their local effects, bioavailability, biocompatibility and antimicrobial activity. To test whether liposomes could enhance the antichlamydial action of AZT, we encapsulated AZT in different surface-charged elastic liposomes (neutral, cationic and anionic elastic liposomes) and assessed their antibacterial potential against the C. trachomatis serovar D laboratory strain as well as the clinical isolate C. trachomatis serovar F. A direct quantitative polymerase chain reaction (qPCR) method was used to measure chlamydial genome content 48 h post infection and to determine the recoverable chlamydial growth. All the liposomes efficiently delivered AZT to HeLa 229 cells infected with the laboratory Chlamydia strain, exhibiting the minimal inhibitory concentrations (MIC) and the minimal bactericidal concentrations (MBC) of AZT even 4–8-fold lower than those achieved with the free AZT. The tested AZT-liposomes were also effective against the clinical Chlamydia strain by decreasing MIC values by 2-fold relative to the free AZT. Interestingly, the neutral AZT-liposomes had no effect on the MBC against the clinical strain, while cationic and anionic AZT-liposomes decreased the MBC 2-fold, hence proving the potential of the surface-charged elastic liposomes to improve the effectiveness of AZT against C. trachomatis.
36
Hussain, Afzal, Mohammad A. Altamimi, Obaid Afzal, Abdulmalik S. A. Altamimi, Mohhammad Ramzan, and Tahir Khuroo. "Mechanistic of Vesicular Ethosomes and Elastic Liposomes on Permeation Profiles of Acyclovir across Artificial Membrane, Human Cultured EpiDerm, and Rat Skin: In Vitro-Ex Vivo Study." Pharmaceutics 15, no. 9 (2023): 2189. http://dx.doi.org/10.3390/pharmaceutics15092189.
Full textAbstract:
Acyclovir (ACV) controls cutaneous herpes, genital herpes, herpes keratitis, varicella zoster, and chickenpox. From previously reported ACV formulations, we continued to explore the permeation behavior of the optimized ACV loaded optimized ethosome (ETHO2R) and elastic liposome (ELP3R) and their respective carbopol gels across artificial membrane, cultured human EpiDerm, and rat skin. Transepidermal water loss (TEWL), scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and atomic force microscopy (AFM) were used to investigate the mechanistic perspective of permeation behavior. The size values of reformulated ELP3-R and ETHO2-R were observed as 217 and 128 nm, respectively (close to previous report), whereas their respective gels showed as 231 and 252 nm, respectively. ETHO2R showed high elasticity, %EE, and low vesicle size. These were investigated for the diffusion rate of the drug permeation (3 h) across the artificial membrane, cultured human EpiDerm, and rat skin. ETHO2GR showed the highest permeation flux (78.42 µg/cm2/h), diffusion coefficient (8.24 × 10−5 cm2/h), and permeation coefficient (0.67 × 10−3 cm/h) of ACV across synthetic membrane, whereas diffusion coefficient (2.4 × 10−4 cm2/h) and permeation coefficient (0.8 × 10−3 cm/h) were maximum across EpiDerm for ETHO2GR. ETHO2R suspension showed maximized permeation flux (169.58 µg/cm2/h) and diffusion rate (0.293 mg/cm2/h1/2), suggesting the rapid internalization of vesicles with cultured skin cells at low viscosity. A similar observation was revealed using rat skin, wherein the permeation flux (182.42 µg/cm2/h), permeation coefficient (0.3 × 10−2 cm/h), and diffusion rate (0.315 mg/cm2/h1/2) of ETHO2R were relatively higher than ELP3R and ELP3GR. Relative small size (128 nm), low viscosity, ethanol-mediated ultra-deformability, high drug entrapment (98%), and elasticity (63.2) are associated with ETHO2R to provide remarkable permeation behavior across the three barriers. The value of TEWL for ETHO2R (21.9 g/m2h) was 3.71 times higher than untreated control (5.9 g/m2h), indicating ethanol-mediated maximized surficial skin lipid perturbation at 3 h of application, whereas the respective ETHO2GR-treated rat skin had TEWL value (18.6 g/m2h) slightly lower than ETHO2R due to gel-based hydration into the skin. SEL, CLSM, and AFM provided a mechanistic perspective of ETHO2R and ELP3R-mediated permeation across rat skin and carrier-mediated visualization (skin–vesicle interaction). AFM provided detailed nanoscale surface roughness topographical parameters of treated and untreated rat skin as supportive data to SEM and CLSM. Thus, ethosomes ETHO2R and respective gel assisted maximum permeation of ACV across rat skin and cultured human EpiDerm to control cutaneous herpes infection and herpes keratitis.
37
Duangjit, Sureewan, Praneet Opanasopit, Theerasak Rojanarata, and Tanasait Ngawhirunpat. "Effect of Edge Activator on Characteristic and in Vitro Skin Permeation of Meloxicam Loaded in Elastic Liposomes." Advanced Materials Research 194-196 (February 2011): 537–40. http://dx.doi.org/10.4028/www.scientific.net/amr.194-196.537.
Full textAbstract:
The aim of this study was to prepare and investigate the potential use of liposomes in the transdermal drug delivery of meloxicam (MX). The vesicles containing a constant amount of MX, phosphatidylcholine (PC), cholesterol (Chol) and cetylpyridinium chloride (CPC) (1:5:1:1 MX/PC/Chol/CPC molar ratio) to obtain liposomes. MX loaded liposomes were investigated for particle size, zeta potential, entrapment efficiency (%EE) and in vitro skin permeation. The results indicated that the liposomes were spherical in structure, 77 to 100 nm in size and charged. The %EE of MX in the vesicles ranged from 55 to 56%. The elastic liposomes consisting of MX/PC/Chol/CPC provided a significantly higher skin permeation of MX compared to the other formulations. Fourier Transform Infrared Spectroscopy (FT-IR) and Differential Scanning Calorimetry (DSC) analysis indicated that the application of liposomes may disrupt the stratum corneum lipid. Our research suggests that MX loaded elastic liposomes can be potentially used as a transdermal drug delivery system.
38
Kumar, Abhay, Amit Nayak, and Sailesh Kumar Ghatuary. "Design, Optimization and Characterization of a Transferosomal Gel of Acyclovir for Effective Treatment of Herpes Zoster." Journal of Drug Delivery and Therapeutics 9, no. 4-A (2019): 712–21. http://dx.doi.org/10.22270/jddt.v9i4-a.3556.
Full textAbstract:
A transferosome is the first generation of an elastic liposome prepared from phospholipids and edge activators. An edge activator is often a single-chain surfactant with a high radius of curvature that destabilizes the lipid bilayers of vesicles and increases the deformability of the bilayers, thereby making the vehicle ultra-deformable. Acyclovir is a synthetic purine nucleoside analog derived from guanine, is the most widely used antiviral agent. It is effective in the treatment of herpes simplex virus (HSV), mainly HSV-1 and HSV-2 and varicella-zoster virus. However, it has low skin permeability. Hence, the objective of this study was to prepare acyclovir using transferosomes to overcome the barrier function of the skin. The present study deals with the development of transferosomal gel containing acyclovir by handshaking method for painless acyclovir delivery for use in the treatment of skin disease through 33 Fractional factorial design in which amount of Phospholipid (A), Cholesterol (B) and Tween 80 (C) was selected as independent variables and vesicle size (X1) Polydispersity index (X2) and %entrapment efficiency (X3) as dependent variables. The prepared transferosomes were evaluated with respect to entrapment efficiency (EE %), particle size, and quantity of in vitro drug released to obtain an optimized formulation. The optimized formulation of acyclovir transferosomes was incorporated into a Carbapol 934 gel base which was evaluated for drug content, pH, spreadability, viscosity and in vitro permeation. The prepared acyclovir transferosomes had a high EE% ranging from 65 to 81%, with small particle sizes ranging from 181.9 to 401.8nm. The in vitro release study suggested that there was an inverse relationship between EE% and in vitro release. The formulation TF2 have better in-vitro drug release profile which contains carbopol 980 concentration 2 %w/w. The kinetic analysis of release profiles of TF2 was found to follow the Korsmeyer-Peppas model. All independent variables had a significant effect on the dependent variables (p-values < 0.05). Therefore, acyclovir in the form of transferosomes can penetrate the skin, overcoming the stratum corneum barrier.
39
Souto, Eliana B., Ana S. Macedo, João Dias-Ferreira, Amanda Cano, Aleksandra Zielińska, and Carla M. Matos. "Elastic and Ultradeformable Liposomes for Transdermal Delivery of Active Pharmaceutical Ingredients (APIs)." International Journal of Molecular Sciences 22, no. 18 (2021): 9743. http://dx.doi.org/10.3390/ijms22189743.
Full textAbstract:
Administration of active pharmaceutical ingredients (APIs) through the skin, by means of topical drug delivery systems, is an advanced therapeutic approach. As the skin is the largest organ of the human body, primarily acting as a natural protective barrier against permeation of xenobiotics, specific strategies to overcome this barrier are needed. Liposomes are nanometric-sized delivery systems composed of phospholipids, which are key components of cell membranes, making liposomes well tolerated and devoid of toxicity. As their lipid compositions are similar to those of the skin, liposomes are used as topical, dermal, and transdermal delivery systems. However, permeation of the first generation of liposomes through the skin posed some limitations; thus, a second generation of liposomes has emerged, overcoming permeability problems. Various mechanisms of permeation/penetration of elastic/ultra-deformable liposomes into the skin have been proposed; however, debate continues on their extent/mechanisms of permeation/penetration. In vivo bioavailability of an API administered in the form of ultra-deformable liposomes is similar to the bioavailability achieved when the same API is administered in the form of a solution by subcutaneous or epi-cutaneous injection, which demonstrates their applicability in transdermal drug delivery.
40
Athanasopoulou, Sophia, Eleni Spanidi, Eleni Panagiotidou, Andrea Cavagnino, Anaïs Bobier, and Konstantinos Gardikis. "An Advanced Combinatorial System from Vitis vinifera Leaves and Propolis Enhances Antioxidants’ Skin Delivery and Fibroblasts Functionality." Pharmaceuticals 17, no. 12 (2024): 1610. http://dx.doi.org/10.3390/ph17121610.
Full textAbstract:
Background/Objectives: Vine leaves are a bulky by-product that are disposed of and treated as waste in the wine production process. In the present study polyphenols from vine leaves were extracted and simultaneously encapsulated in a new delivery system consisting of liposomes and cyclodextrins. This system was further combined with propolis polyphenols encapsulated in cyclodextrins, resulting in a colloidal suspension for the release of antioxidants in a time-controlled way, the rate of which depends on the ratio of the materials. The result is a raw material that exhibits antioxidant and ECM protective effects when administered in skin fibroblasts (NHDFs). Methods: The antioxidant and ECM promoting efficacy of the produced raw material was assessed by the Folin–Ciocalteu method, DPPH assay, and in cellulo assays in fibroblasts, such as the cell viability assay, scratch assay, cell migration assay, gene expression analysis, and immunofluorescence analysis, for the detection, visualization, and quantification of collagen-I, collagen-IIIa, and elastin signals and collagenase assay. Results: Treatment of NHDFs with the combinatorial delivery system promoted collagen and elastin synthesis and deposition in normal conditions and, upon induced external stress, as assessed by in vitro transcriptomic and proteomic analysis. A significant inhibition of collagenase was also observed, suggesting a multitargeted efficacy of the active ingredients also by preventing collagen degradation. Conclusions: Therefore, this liposome–cyclodextrin encapsulated polyphenol complex represents a novel bioactive ingredient with promising skin applications.
41
Bashyal, Santosh, Jo-Eun Seo, Taekwang Keum, Gyubin Noh, Shrawani Lamichhane, and Sangkil Lee. "Development, Characterization, and Ex Vivo Assessment of Elastic Liposomes for Enhancing the Buccal Delivery of Insulin." Pharmaceutics 13, no. 4 (2021): 565. http://dx.doi.org/10.3390/pharmaceutics13040565.
Full textAbstract:
Buccal drug delivery is a suitable alternative to invasive routes of drug administration. The buccal administration of insulin for the management of diabetes has received substantial attention worldwide. The main aim of this study was to develop and characterize elastic liposomes and assess their permeability across porcine buccal tissues. Sodium-cholate-incorporated elastic liposomes (SC-EL) and sodium-glycodeoxycholate-incorporated elastic liposomes (SGDC-EL) were prepared using the thin-film hydration method. The prepared liposomes were characterized and their ex vivo permeability attributes were investigated. The distribution of the SC-EL and SGDC-EL across porcine buccal tissues was evaluated using confocal laser scanning microscopy (CLSM). The SGDC-EL were the most superior nanocarriers since they significantly enhanced the permeation of insulin across porcine buccal tissues, displaying a 4.33-fold increase in the permeability coefficient compared with the insulin solution. Compared with the SC-EL, the SGDC-EL were better at facilitating insulin permeability, with a 3.70-fold increase in the permeability coefficient across porcine buccal tissue. These findings were further corroborated based on bioimaging analysis using CLSM. SGDC-ELs showed the greatest fluorescence intensity in buccal tissues, as evidenced by the greater shift of fluorescence intensity toward the inner buccal tissue over time. The fluorescence intensity ranked as follows: SGDC-EL > SC-EL > FITC–insulin solution. Conclusively, this study highlighted the potential nanocarriers for enhancing the buccal permeability of insulin.
42
Gupta, Sudipta, and Gerald J. Schneider. "Modeling the dynamics of phospholipids in the fluid phase of liposomes." Soft Matter 16, no. 13 (2020): 3245–56. http://dx.doi.org/10.1039/c9sm02111f.
Full text
43
Calienni, Maria Natalia, Luis Manuel Martínez, Maria Cecilia Izquierdo, Silvia del Valle Alonso, and Jorge Montanari. "Rheological and Viscoelastic Analysis of Hybrid Formulations for Topical Application." Pharmaceutics 15, no. 10 (2023): 2392. http://dx.doi.org/10.3390/pharmaceutics15102392.
Full textAbstract:
The rheological and viscoelastic properties of hybrid formulations composed of vehicles designed for cutaneous topical application and loaded with ultradeformable liposomes (UDL) were assessed. UDL were selected for their established ability to transport both lipophilic and hydrophilic compounds through the skin, and are applicable in pharmaceuticals and cosmetics. Formulations underwent flow analysis and were fitted to the Herschel–Bulkley model due to their prevalent non-Newtonian behavior in most cases. Linear viscoelastic regions (LVR) were identified, and G′ and G″ moduli were determined via frequency sweep steps, considering the impact of temperature and aging. The formulations exhibited non-Newtonian behavior with pseudoplastic traits in most cases, with UDL incorporation inducing rheological changes. LVR and frequency sweep tests indicated predominantly elastic solid behavior, with G′ higher than G″, at different temperatures and post-production times. Tan δ values also illustrated a predominant solid-like behavior over liquid. This study provides pivotal insights into the rheological and viscoelastic features of topical formulations, emphasizing the crucial role of meticulous vehicle and formulation selection when incorporating UDL or analogous liposomal drug delivery systems.
44
Benson, Heather. "Elastic Liposomes for Topical and Transdermal Drug Delivery." Current Drug Delivery 6, no. 3 (2009): 217–26. http://dx.doi.org/10.2174/156720109788680813.
Full text
45
MISHRA, D., V. DUBEY, A. ASTHANA, D. SARAF, and N. JAIN. "Elastic liposomes mediated transcutaneous immunization against Hepatitis B." Vaccine 24, no. 22 (2006): 4847–55. http://dx.doi.org/10.1016/j.vaccine.2006.03.011.
Full text
46
Trotta, M., E. Peira, F. Debernardi, and M. Gallarate. "Elastic liposomes for skin delivery of dipotassium glycyrrhizinate." International Journal of Pharmaceutics 241, no. 2 (2002): 319–27. http://dx.doi.org/10.1016/s0378-5173(02)00266-1.
Full text
47
Cadena, Pabyton G., Marcela A. Pereira, Rafaela B. S. Cordeiro, et al. "Nanoencapsulation of quercetin and resveratrol into elastic liposomes." Biochimica et Biophysica Acta (BBA) - Biomembranes 1828, no. 2 (2013): 309–16. http://dx.doi.org/10.1016/j.bbamem.2012.10.022.
Full text
48
Pamplona, D. C., and C. R. Calladine. "The Mechanics of Axially Symmetric Liposomes." Journal of Biomechanical Engineering 115, no. 2 (1993): 149–59. http://dx.doi.org/10.1115/1.2894115.
Full textAbstract:
Hotani has filmed morphological transformations in unilamellar liposomes, starting from a spherical shape, when the interior volume decreases steadily. Hotani’s liposomes showed no evidence of general thermal fluctuations. We use a finite-deformation theory of axisymmetric, quasi-static thin shells to analyze theoretically bifurcations and changes of shape in liposomes under decreasing volume. The main structural action in a lipid bilayer is generally agreed to be its elastic resistance to bending, and it is usual to regard surface deformation as being like that of a two-dimensional liquid. We find, however, that some in-plane shear elasticity is also needed in order to produce the observed post-bifurcation behavior. Such an elasticity would be difficult to measure directly.
49
Jain, Sanjay, Yashwant Gupta, Anekant Jain, and Sadia Amin. "Elastic Liposomes Bearing Meloxicam-β -Cyclodextrin for Transdermal Delivery." Current Drug Delivery 5, no. 3 (2008): 207–14. http://dx.doi.org/10.2174/156720108784911677.
Full text
50
Cereda, Cintia Maria Saia, Michelle Franz-Montan, Camila Morais Gonçalves da Silva, et al. "Transdermal delivery of butamben using elastic and conventional liposomes." Journal of Liposome Research 23, no. 3 (2013): 228–34. http://dx.doi.org/10.3109/08982104.2013.796975.
Full text