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Journal articles on the topic 'Electrical Plaque Reduction Neutralization Test'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

Flaegstad, Trond, Terje Traavik, Karen Elina Christie, and Jorunn Joergensen. "Neutralization test for bk virus: plaque reduction detected by immunoperoxidase staining." Journal of Medical Virology 19, no. 3 (1986): 287–96. http://dx.doi.org/10.1002/jmv.1890190311.

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2

Di Gennaro, Annapia, Alessio Lorusso, Claudia Casaccia, Annamaria Conte, Federica Monaco, and Giovanni Savini. "Serum Neutralization Assay Can Efficiently Replace Plaque Reduction Neutralization Test for Detection and Quantitation of West Nile Virus Antibodies in Human and Animal Serum Samples." Clinical and Vaccine Immunology 21, no. 10 (2014): 1460–62. http://dx.doi.org/10.1128/cvi.00426-14.

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ABSTRACTA serum neutralization assay (SN) was compared with the official plaque reduction neutralization test for the quantitation of West Nile virus antibodies. A total of 1,348 samples from equid sera and 38 from human sera were tested by these two methods. Statistically significant differences were not observed, thus supporting the use of SN for routine purposes.

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3

Lee, Hee-Jung, Kyung-Il Min, Ki Hoon Park, et al. "Comparison of JEV neutralization assay using pseudotyped JEV with the conventional plaque-reduction neutralization test." Journal of Microbiology 52, no. 5 (2014): 435–40. http://dx.doi.org/10.1007/s12275-014-3529-y.

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4

Brown, Jerry F., John M. Dye, Sam Tozay, et al. "Anti–Ebola Virus Antibody Levels in Convalescent Plasma and Viral Load After Plasma Infusion in Patients With Ebola Virus Disease." Journal of Infectious Diseases 218, no. 4 (2018): 555–62. http://dx.doi.org/10.1093/infdis/jiy199.

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Enzyme-linked immunosorbent and microneutralization assays of 180 Ebola convalescent plasma specimens were highly concordant and predictive for detection of antibody by 50% plaque reduction neutralization test. Viral load decreased following infusion of antibody-containing plasma in 2 Ebola virus disease patients.

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5

Vaidya, Sunil R. "Immuno-Colorimetric Neutralization Test: A Surrogate for Widely Used Plaque Reduction Neutralization Tests in Public Health Virology." Viruses 15, no. 4 (2023): 939. http://dx.doi.org/10.3390/v15040939.

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Since their first documentation in 1952, plaque reduction neutralization tests (PRNTs) have become the choice of test for the measurement of neutralizing antibodies against a particular virus. However, PRNTs can be performed only against viruses that cause cytopathic effects (CPE). PRNTs also require skilled personnel and can be time-consuming depending on the time required for the virus to cause CPE. Hence, their application limits large-scale studies or epidemiological and laboratory investigations. Since 1978, many surrogate PRNTs or immunocolorimetric assay (ICA)-based focus reduction neut

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6

Morens, D. M., S. B. Halstead, P. M. Repik, R. Putvatana, and N. Raybourne. "Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization." Journal of Clinical Microbiology 22, no. 2 (1985): 250–54. http://dx.doi.org/10.1128/jcm.22.2.250-254.1985.

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7

Borges, Maria Beatriz J., Sayuri E. M. Kato, Clarissa R. A. Damaso, et al. "Accuracy and repeatability of a micro plaque reduction neutralization test for vaccinia antibodies." Biologicals 36, no. 2 (2008): 105–10. http://dx.doi.org/10.1016/j.biologicals.2007.07.001.

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8

Komar, Nicholas, Stanley Langevin, and Thomas P. Monath. "Use of a Surrogate Chimeric Virus To Detect West Nile Virus-Neutralizing Antibodies in Avian and Equine Sera." Clinical and Vaccine Immunology 16, no. 1 (2008): 134–35. http://dx.doi.org/10.1128/cvi.00220-08.

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ABSTRACT A chimeric yellow fever virus/West Nile virus (WNV) was compared to WNV alone as a biosafety level 2 reagent in the plaque reduction neutralization test for determining WNV infection histories. Concordance was 96.3% among 188 avian and equine serum samples. Neutralizing antibody titers were frequently more than twofold lower with the chimera.

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9

Thanh, Le Thi, Hoang Vu Mai Phuong, Nguyen Le Khanh Hang, et al. "The neutralization antibodies of SARS-CoV-2 during COVID-19 outbreak in Da Nang, Vietnam, July - August 2020." Tạp chí Y học Dự phòng 33, no. 8 (2024): 16–23. http://dx.doi.org/10.51403/0868-2836/2023/1473.

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Beside molecular tests (realtime RT-PCR) which known as the golden key for SARS-CoV-2 confirmation, antibody test can be used to determine the responses in human antibody to SARS-CoV-2 and the result of plaque reduction neutralization test (PRNT) could identify the people who have been exposed with the SARS-CoV-2 and carried the neutralization antibody. Total 9936 samples were collected in 3 groups healthcare workers, inpatients (not related to COVID-19 before) and community in Da Nang city during July-August 2020. These cross sectional results showed that the rate of neutralization antibody i

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10

Brugha, R., M. Ramsay, T. Forsey, and D. Brown. "A study of maternally derived measles antibody in infants born to naturally infected and vaccinated women." Epidemiology and Infection 117, no. 3 (1996): 519–24. http://dx.doi.org/10.1017/s0950268800059203.

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SummaryMaternal, cord and infant measles antibody levels were measured and compared in a group of 411 vaccinated mothers and 240 unvaccinated mothers, and their babies, between 1983 and 1991. Maternal and cord sera were tested by haemagglutination inhibition and/or enzyme-linked immunosorbent assay, and plaque reduction neutralization tests were also used to test infant sera. Geometric mean litres were significantly higher in the unvaccinated than in the vaccinated mothers (P< 0·001). Infants born to mothers with a history of measles had higher antibody levels at birth than infants of vacci

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11

Yeh, Chia-Tsui, Chia-Ying Lee, Yi-Jung Ho, et al. "Immunoglobulin Y Specific for SARS-CoV-2 Spike Protein Subunits Effectively Neutralizes SARS-CoV-2 Infectivity and Ameliorates Disease Manifestations In Vivo." Biomedicines 10, no. 11 (2022): 2774. http://dx.doi.org/10.3390/biomedicines10112774.

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(Background) The coronavirus disease 2019 (COVID-19) that is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) carries high infectivity and mortality. Efficient intervention strategies are urgently needed. Avian immunoglobulin Y (IgY) showed efficacy against viral infection whereas the in vivo efficacy remains unclear. (Methods) We immunized laying hens with S1, S1 receptor-binding domain (S1-RBD), or S2 subunits of the SARS-CoV-2 spike (S) protein. After immunization, IgYs were collected and extracted from the egg yolks. The neutralization potential of IgYs was examined b

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12

Zou, Jing, Hongjie Xia, Pei-Yong Shi, Xuping Xie, and Ping Ren. "A Single-Round Infection Fluorescent SARS-CoV-2 Neutralization Test for COVID-19 Serological Testing at a Biosafety Level-2 Laboratory." Viruses 14, no. 6 (2022): 1211. http://dx.doi.org/10.3390/v14061211.

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A robust serological test to measure neutralizing antibodies against SARS-CoV-2 in biosafety level-2 (BSL-2) laboratories is useful for monitoring antibody response after vaccination or natural infection. The gold standard assay is the conventional plaque reduction neutralization test (PRNT) which requires extensive labor, live viruses, and BSL-3 facilities. Recently, we developed a novel single-round infection fluorescent SARS-CoV-2 virus (SFV) that can be safely used at BSL-2 laboratories for high-throughput neutralization and antiviral testing. In this study, we evaluated the performance of

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13

Johnson, Alison J., Amanda J. Noga, Olga Kosoy, Robert S. Lanciotti, Alicia A. Johnson, and Brad J. Biggerstaff. "Duplex Microsphere-Based Immunoassay for Detection of Anti-West Nile Virus and Anti-St. Louis Encephalitis Virus Immunoglobulin M Antibodies." Clinical Diagnostic Laboratory Immunology 12, no. 5 (2005): 566–74. http://dx.doi.org/10.1128/cdli.12.5.566-574.2005.

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ABSTRACT West Nile (WN) virus was introduced into the United States in 1999, when the first human cases of WN fever and encephalitis appeared in New York City. From there, the virus has spread throughout North America, in some areas cocirculating with the related flavivirus St. Louis encephalitis (SLE) virus. Public health laboratories currently use an immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) as a primary test for human serodiagnosis, followed by a confirmatory plaque-reduction neutralization test (PRNT). The MAC-ELISAs take 2 days to perform; there

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14

Gibbs, Samantha E. J., Douglas M. Hoffman, Lillian M. Stark, et al. "Persistence of Antibodies to West Nile Virus in Naturally Infected Rock Pigeons (Columba livia)." Clinical Diagnostic Laboratory Immunology 12, no. 5 (2005): 665–67. http://dx.doi.org/10.1128/cdli.12.5.665-667.2005.

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ABSTRACT Wild caught rock pigeons (Columba livia) with antibodies to West Nile virus were monitored for 15 months to determine antibody persistence and compare results of three serologic techniques. Antibodies persisted for the entire study as detected by epitope-blocking enzyme-linked immunosorbent assay and plaque reduction neutralization test. Maternal antibodies in squabs derived from seropositive birds persisted for an average of 27 days.

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15

Ko, Jae-Hoon, Ji Yeon Lee, Jin Yang Baek, et al. "Serologic Evaluation of MERS Screening Strategy for Healthcare Personnel During a Hospital-Associated Outbreak." Infection Control & Hospital Epidemiology 38, no. 2 (2016): 234–38. http://dx.doi.org/10.1017/ice.2016.251.

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To evaluate the appropriateness of the screening strategy for healthcare personnel (HCP) during a hospital-associated Middle East Respiratory Syndrome (MERS) outbreak, we performed a serologic investigation in 189 rRT-PCR–negative HCP exposed and assigned to MERS patients. Although 20%–25% of HCP experienced MERS-like symptoms, none of them showed seroconversion by plaque reduction neutralization test (PRNT).Infect Control Hosp Epidemiol 2017;38:234–238

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16

Meegan, J. M., R. J. Yedloutschnig, B. A. Peleg, et al. "Enzyme-linked immunosorbent assay for detection of antibodies to Rift Valley fever virus in ovine and bovine sera." American Journal of Veterinary Research 48, no. 7 (1987): 1138–41. https://doi.org/10.2460/ajvr.1987.48.07.1138.

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SUMMARY An enzyme-linked immunosorbent assay (elisa) was developed for the rapid detection of antibodies to Rift Valley fever virus (rvfv) in ovine and bovine sera. Conditions to reduce nonspecific reactions were optimized. The elisa results correlated with those of a plaque-reduction neutralization test, revealing 100% specificity and 90.7% sensitivity. In sera from sheep and cattle inoculated against rvfv, the hemagglutination-inhibition test in combination with the elisa provided a better indication of response to killed rvfv vaccine than did either test alone.

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17

Guo, Jiazheng, Jiansheng Lu, Peng Du, et al. "Fluorescence Reduction Neutralization Test: A Novel, Rapid, and Efficient Method for Characterizing the Neutralizing Activity of Antibodies Against Dengue Virus." Current Issues in Molecular Biology 47, no. 3 (2025): 140. https://doi.org/10.3390/cimb47030140.

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Dengue virus (DENV) is a major public health threat in the tropical and subtropical regions of the world. Climate change resulting from global warming is further expanding DENV–endemic areas, adversely affecting public life and health. Despite this, no specific drug against DENV has been developed so far. Vaccines and neutralizing antibodies are the chief preventive and therapeutic tools for managing pathogenic infections. The present study describes the development of a novel fluorescence reduction neutralization test (FRNT) for evaluating the neutralizing activity of antibodies against DENV.

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18

Zou, Jing, Chaitanya Kurhade, Hope C. Chang, et al. "An Integrated Research–Clinical BSL-2 Platform for a Live SARS-CoV-2 Neutralization Assay." Viruses 15, no. 9 (2023): 1855. http://dx.doi.org/10.3390/v15091855.

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A reliable and efficient serological test is crucial for monitoring neutralizing antibodies against SARS-CoV-2 and its variants of concern (VOCs). Here, we present an integrated research–clinical platform for a live SARS-CoV-2 neutralization assay, utilizing highly attenuated SARS-CoV-2 (Δ3678_WA1-spike). This strain contains mutations in viral transcription regulation sequences and deletion in the open-reading-frames 3, 6, 7, and 8, allowing for safe handling in biosafety level 2 (BSL-2) laboratories. Building on this backbone, we constructed a genetically stable reporter virus (mGFP Δ3678_WA

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19

Qu, Shenghua, Xiaoyan Wang, Lixin Yang, et al. "Identification of a Neutralizing Monoclonal Antibody That Recognizes a Unique Epitope on Domain III of the Envelope Protein of Tembusu Virus." Viruses 12, no. 6 (2020): 647. http://dx.doi.org/10.3390/v12060647.

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Domain III of the envelope protein (EDIII) is the major target of flavivirus neutralizing antibody. To date, little is known regarding antibody-mediated neutralization of Tembusu virus (TMUV), a novel flavivirus emerging in duck in 2010. Here, a novel monoclonal antibody (MAb), designated 12F11, was prepared by immunization of mice with recombinant EDIII (rEDIII) protein. Using virus neutralization test, 12F11 in undiluted ascites neutralized the TMUV infectivity to induce the development of cytopathic effects in BHK-21 cells, indicating that 12F11 exhibits a neutralizing activity. The neutral

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20

Xie, Xuping, Marisa C. Nielsen, Antonio E. Muruato, Camila R. Fontes-Garfias, and Ping Ren. "Evaluation of a SARS-CoV-2 lateral flow assay using the plaque reduction neutralization test." Diagnostic Microbiology and Infectious Disease 99, no. 2 (2021): 115248. http://dx.doi.org/10.1016/j.diagmicrobio.2020.115248.

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21

Horbach, Ingrid Siciliano, Adriana de Souza Azevedo, Waleska Dias Schwarcz, et al. "Plaque Reduction Neutralization Test (PRNT) Accuracy in Evaluating Humoral Immune Response to SARS-CoV-2." Diseases 12, no. 1 (2024): 29. http://dx.doi.org/10.3390/diseases12010029.

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Massive vaccination positively impacted the SARS-CoV-2 pandemic, being a strategy to increase the titers of neutralizing antibodies (NAbs) in the population. Assessing NAb levels and understanding the kinetics of NAb responses is critical for evaluating immune protection. In this study, we optimized and validated a PRNT50 assay to assess 50% virus neutralization and evaluated its accuracy to measure NAbs to the original strain or variant of SARS-CoV-2. The optimal settings were selected, such as the cell (2 × 105 cells/well) and CMC (1.5%) concentrations and the viral input (~60 PFU/well) for

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22

Konduru, Krishnamurthy, Amy C. Shurtleff, Sina Bavari, and Gerardo Kaplan. "High degree of correlation between Ebola virus BSL-4 neutralization assays and pseudotyped VSV BSL-2 fluorescence reduction neutralization test." Journal of virological methods 254 (June 12, 2018): 1–7. https://doi.org/10.5281/zenodo.13535264.

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(Uploaded by Plazi for the Bat Literature Project) Ebola virus (EBOV), classified as a category A agent by the CDC and NIH, requires BSL-4 containment and induces high morbidity and mortality in humans. The 2013–2015 epidemic in West Africa underscored the urgent need to develop vaccines and therapeutics to prevent and treat EBOV disease. Neutralization assays are needed to evaluate the efficacy of EBOV vaccines and antibody therapies. Pseudotyped viruses based on nonpathogenic or attenuated vectors reduce the risks involved in the evaluation of neutralizing antibodies against highly pathogeni

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23

Konduru, Krishnamurthy, Amy C. Shurtleff, Sina Bavari, and Gerardo Kaplan. "High degree of correlation between Ebola virus BSL-4 neutralization assays and pseudotyped VSV BSL-2 fluorescence reduction neutralization test." Journal of virological methods 254 (June 7, 2018): 1–7. https://doi.org/10.5281/zenodo.13535264.

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(Uploaded by Plazi for the Bat Literature Project) Ebola virus (EBOV), classified as a category A agent by the CDC and NIH, requires BSL-4 containment and induces high morbidity and mortality in humans. The 2013–2015 epidemic in West Africa underscored the urgent need to develop vaccines and therapeutics to prevent and treat EBOV disease. Neutralization assays are needed to evaluate the efficacy of EBOV vaccines and antibody therapies. Pseudotyped viruses based on nonpathogenic or attenuated vectors reduce the risks involved in the evaluation of neutralizing antibodies against highly pathogeni

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24

Johnson, Barbara W., Olga Kosoy, Elizabeth Hunsperger, et al. "Evaluation of Chimeric Japanese Encephalitis and Dengue Viruses for Use in Diagnostic Plaque Reduction Neutralization Tests." Clinical and Vaccine Immunology 16, no. 7 (2009): 1052–59. http://dx.doi.org/10.1128/cvi.00095-09.

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ABSTRACT The plaque reduction neutralization test (PRNT) is a specific serological test used to identify and confirm arbovirus infection in diagnostic laboratories and monitor immunological protection in vaccine recipients. Wild-type (wt) viruses used in the PRNT may be difficult to grow and plaque titrate, such as the dengue viruses (DENV), and/or may require biosafety level 3 (BSL3) containment, such as West Nile virus (WNV), St. Louis encephalitis virus (SLEV), and Japanese encephalitis virus (JEV). These requirements preclude their use in diagnostic laboratories with only BSL2 capacity. In

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25

Yaghoobizad, Somayeh, Zahra Norouzbabaei, Nazanin Zahra Shafiei Jandaghi, et al. "Evaluation of the focus reduction neutralization and ELISA tests compared to the plaque reduction neutralization test for the detection of antibodies against measles virus." Biologicals 88 (November 2024): 101795. http://dx.doi.org/10.1016/j.biologicals.2024.101795.

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26

Amaya, Moushimi, Randy Yin, Lianying Yan, et al. "A Recombinant Chimeric Cedar Virus-Based Surrogate Neutralization Assay Platform for Pathogenic Henipaviruses." Viruses 15, no. 5 (2023): 1077. http://dx.doi.org/10.3390/v15051077.

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The henipaviruses, Nipah virus (NiV), and Hendra virus (HeV) can cause fatal diseases in humans and animals, whereas Cedar virus is a nonpathogenic henipavirus. Here, using a recombinant Cedar virus (rCedV) reverse genetics platform, the fusion (F) and attachment (G) glycoprotein genes of rCedV were replaced with those of NiV-Bangladesh (NiV-B) or HeV, generating replication-competent chimeric viruses (rCedV-NiV-B and rCedV-HeV), both with and without green fluorescent protein (GFP) or luciferase protein genes. The rCedV chimeras induced a Type I interferon response and utilized only ephrin-B2

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27

Haga, Kazumi, Zhenying (Nancy) Chen, Misao Himeno, Ryuichi Majima, and Meng Ling Moi. "Utility of an In-Vitro Micro-Neutralizing Test in Comparison to a Plaque Reduction Neutralization Test for Dengue Virus, Japanese Encephalitis Virus, and Zika Virus Serology and Drug Screening." Pathogens 13, no. 1 (2023): 8. http://dx.doi.org/10.3390/pathogens13010008.

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Flavivirus infections, including dengue virus (DENV), Japanese encephalitis virus (JEV), and Zika virus (ZIKV), present significant global public health challenges. For successful vaccine design, the assessment of neutralizing antibody activity requires reliable and robust methodologies for determining antibody titers. Although the plaque reduction neutralization test (PRNT) is commonly acknowledged as the gold standard, it has limitations in terms of time and cost, and its usage may be limited in resource-limited settings. To address these challenges, we introduced the micro-neutralization te

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28

Postnikova, Elena N., James Pettitt, Ryn Collin J. Van, et al. "Scalable, semi-automated fluorescence reduction neutralization assay for qualitative assessment of Ebola virus-neutralizing antibodies in human clinical samples." PLoS ONE 14, no. 8 (2019): e0221407. https://doi.org/10.5281/zenodo.13533393.

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(Uploaded by Plazi for the Bat Literature Project) Antibody titers against a viral pathogen are typically measured using an antigen binding assay, such as an enzyme-linked immunosorbent assay (ELISA), which only measures the ability of antibodies to identify a viral antigen of interest. Neutralization assays measure the presence of virus-neutralizing antibodies in a sample. Traditional neutralization assays, such as the plaque reduction neutralization test (PRNT), are often difficult to use on a large scale due to being both labor and resource intensive. Here we describe an Ebola virus fluores

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29

Postnikova, Elena N., James Pettitt, Ryn Collin J. Van, et al. "Scalable, semi-automated fluorescence reduction neutralization assay for qualitative assessment of Ebola virus-neutralizing antibodies in human clinical samples." PLoS ONE 14, no. 8 (2019): e0221407. https://doi.org/10.5281/zenodo.13533393.

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(Uploaded by Plazi for the Bat Literature Project) Antibody titers against a viral pathogen are typically measured using an antigen binding assay, such as an enzyme-linked immunosorbent assay (ELISA), which only measures the ability of antibodies to identify a viral antigen of interest. Neutralization assays measure the presence of virus-neutralizing antibodies in a sample. Traditional neutralization assays, such as the plaque reduction neutralization test (PRNT), are often difficult to use on a large scale due to being both labor and resource intensive. Here we describe an Ebola virus fluores

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30

Swanepoel, R., J. K. Struthers, M. J. Erasmus, et al. "Comparison of techniques for demonstrating antibodies to Rift Valley fever virus." Journal of Hygiene 97, no. 2 (1986): 317–29. http://dx.doi.org/10.1017/s0022172400065414.

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SummaryNine serological techniques were compared by monitoring the response to infection with Rift Valley fever (RVF) virus in three sheep. Antibodies were monitored daily for the first 14 days after infection, then weekly and later fortnightly up to week 24. The earliest antibody response was detected in one sheep on day 3 by a plaque reduction neutralization test, and by day 6 antibodies were demonstrable in all three sheep by haemagglutination-inhibition, reversed passive haemagglutination-inhibition, immunodiffusion, indirect immunofluorescence (IF), enzyme-linked immunosorbent assay and n

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31

Frumence, Viranaicken, Gadea, and Desprès. "A GFP Reporter MR766-Based Flow Cytometry Neutralization Test for Rapid Detection of Zika Virus-Neutralizing Antibodies in Serum Specimens." Vaccines 7, no. 3 (2019): 66. http://dx.doi.org/10.3390/vaccines7030066.

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Zika virus (ZIKV) is an emerging arthropod-borne virus of major public health concern. ZIKV infection is responsible for congenital Zika disease and other neurological defects. Antibody-mediated virus neutralization is an essential component of protective antiviral immunity against ZIKV. In the present study, we assessed whether our GFP reporter ZIKV derived from African viral strain MR766 could be useful for the development of a flow cytometry neutralization test (FNT), as an alternative to the conventional plaque-reduction neutralization test (PRNT). To improve the efficacy of GFP-expressing

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Rodrigo, W. W. I. S., D. C. Alcena, Z. Kou та ін. "Difference between the Abilities of Human Fcγ Receptor-Expressing CV-1 Cells To Neutralize American and Asian Genotypes of Dengue Virus 2". Clinical and Vaccine Immunology 16, № 2 (2008): 285–87. http://dx.doi.org/10.1128/cvi.00363-08.

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ABSTRACT Sera from patients involved in a Peruvian outbreak of dengue virus serotype 1 infection cross-neutralized the American genotype of dengue virus serotype 2 up to 100-fold more efficiently than they did the virulent Asian genotype of dengue virus serotype 2, as determined by a plaque reduction neutralization test (PRNT) with CV-1 fibroblasts modified to express human Fcγ receptor CD32. The concordant preferential immune enhancement of the Asian genotype of dengue virus serotype 2 in human monocytes suggests that such a modification might strengthen the correlation between the PRNT titer

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Lukman, Nurhayati, Gustiani Salim, Herman Kosasih, et al. "Comparison of the Hemagglutination Inhibition Test and IgG ELISA in Categorizing Primary and Secondary Dengue Infections Based on the Plaque Reduction Neutralization Test." BioMed Research International 2016 (2016): 1–5. http://dx.doi.org/10.1155/2016/5253842.

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Secondary dengue infection by heterotypic serotypes is associated with severe manifestations of disease, that is, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The World Health Organization (WHO) has recommended criteria based on the hemagglutination inhibition (HI) test to distinguish between primary and secondary dengue infections. Since the HI test has practical limitations and disadvantages, we evaluated the accuracy of WHO HI criteria and compared it with criteria based on an IgG enzyme-linked immunosorbent assay (ELISA) using a plaque reduction neutralization test (PRNT

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De Anda, Jorge Hernández, M. D. Salman, Patricia A. Webb, Thomas J. Keefe, Alejandro Arregín Arévalo, and John Mason. "Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to vesicular stomatitis virus in cattle in an enzootic region of Mexico." American Journal of Veterinary Research 53, no. 4 (1992): 440–43. http://dx.doi.org/10.2460/ajvr.1991.53.04.440.

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Summary An elisa was compared with the plaque-reduction serum neutralization (prsn) test, for detection of vesicular stomatitis virus (vsv) antibodies in cattle in a vesicular stomatitis enzootic region of Mexico. A total of 325 bovine serum samples were screened for vsv antibodies. The prsn test was performed, using Vero cells. The elisa contained gradient-purified vsv Indiana (Lab strain) and vsv New Jersey (Hazelhurst) as the antigens. Regression analysis and weighted kappa statistic were used to estimate measures of agreement between the 2 assays for detection of vsv antibodies. The elisa

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35

Souza, Vanda Akico Ueda Fick de, Cláudio Sérgio Pannuti, Cid Vieira Franco de Godoy, Paul Albrecht, Marta Heloísa Lopes, and João da Silva Mendonça. "Comparison of indirect immunofluorescence test for measles antibodies with haemagglutination inhibition and plaque neutralization tests." Revista do Instituto de Medicina Tropical de São Paulo 32, no. 5 (1990): 360–63. http://dx.doi.org/10.1590/s0036-46651990000500009.

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Indirect Immunofluorescence (IFA), Plaque Reduction Neutralization (PRN) and Haemagglutination Inhibition (HI) tests for measles antibodies were carried out in 197 sera obtained from umbilical cord and vaccinated children. The IFA was also applied to blood samples collected with filter paper. IFA results demonstrated that the test is relatively simple to perform, with good reproducibility for different antigen lots. Good correlation was obtained between IFA, PRN and HI antibody titers. Better correlation was demonstrated with IFA and PRN than with HI and PRN tests. Sensitivity of IFA in detect

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36

Hartlaub, Julia, Markus Keller, and Martin H. Groschup. "Deciphering Antibody Responses to Orthonairoviruses in Ruminants." Microorganisms 9, no. 7 (2021): 1493. http://dx.doi.org/10.3390/microorganisms9071493.

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Antibody cross-reactivities between related viruses are common diagnostic challenges, resulting in reduced diagnostic specificities and sensitivities. In this study, antibody cross-reactions between neglected members of the genus Orthonairovirus—Hazara (HAZV), Dugbe (DUGV), and Nairobi sheep disease orthonairovirus (NSDV)—were investigated. Mono-specific ovine and bovine sera following experimental infections as well immunization trials with HAZV, DUGV, and NSDV were tested in homologous and heterologous virus-specific assays, namely indirect ELISAs based on recombinant N protein, indirect imm

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Bondre, Vijay P., Gajanan N. Sapkal, Prasanna N. Yergolkar, et al. "Genetic characterization of Bagaza virus (BAGV) isolated in India and evidence of anti-BAGV antibodies in sera collected from encephalitis patients." Journal of General Virology 90, no. 11 (2009): 2644–49. http://dx.doi.org/10.1099/vir.0.012336-0.

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During investigations into the outbreak of encephalitis in 1996 in the Kerala state in India, an arbovirus was isolated from a Culex tritaeniorhynchus mosquito pool. It was characterized as a Japanese encephalitis and West Nile virus cross-reactive arbovirus by complement fixation test. A plaque reduction–neutralization test was performed using hyperimmune sera raised against the plaque-purified arbovirus isolate. The sera did not show reactivity with Japanese encephalitis virus and were weakly reactive with West Nile virus. Complete open reading frame sequence analysis characterized the arbov

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Arankalle, VidyaA, Ruta Kulkarni, Shubham Shrivastava, et al. "Performance assessment of SARS-CoV-2 IgM & IgG ELISAs in comparison with plaque reduction neutralization test." Indian Journal of Medical Research 153, no. 5 (2021): 658. http://dx.doi.org/10.4103/ijmr.ijmr_3806_20.

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Ratnam, S., V. Gadag, R. West, et al. "Comparison of commercial enzyme immunoassay kits with plaque reduction neutralization test for detection of measles virus antibody." Journal of clinical microbiology 33, no. 4 (1995): 811–15. http://dx.doi.org/10.1128/jcm.33.4.811-815.1995.

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Balingit, Jean Claude, Minh Huong Phu Ly, Mami Matsuda, et al. "A Simple and High-Throughput ELISA-Based Neutralization Assay for the Determination of Anti-Flavivirus Neutralizing Antibodies." Vaccines 8, no. 2 (2020): 297. http://dx.doi.org/10.3390/vaccines8020297.

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Mosquito-borne flavivirus infections, including dengue virus and Zika virus, are major public health threats globally. While the plaque reduction neutralization test (PRNT) is considered the gold standard for determining neutralizing antibody levels to flaviviruses, the assay is time-consuming and laborious. This study, therefore, aimed to develop an enzyme-linked immunosorbent assay (ELISA)-based microneutralization test (EMNT) for the detection of neutralizing antibodies to mosquito-borne flaviviruses. The inhibition of viral growth due to neutralizing antibodies was determined colorimetrica

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Costa, Thaís Alkifeles, Matheus Soares Arruda, Gabriela Fernanda Garcia Oliveira, et al. "Detection of neutralizing antibodies against arboviruses from liver homogenates." PLOS Neglected Tropical Diseases 18, no. 12 (2024): e0012740. https://doi.org/10.1371/journal.pntd.0012740.

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Yellow fever virus (YFV) circulates in a sylvatic cycle between non-human primates (NHPs) and arboreal mosquitoes in Brazil. Passive monitoring of ill or deceased NHPs is a key component of the Brazilian yellow fever (YF) surveillance program. Samples from NHPs carcasses are usually suitable for molecular tests but not for serological assays. As an alternative to the conventional plaque reduction neutralization test (PRNT) based on sera, we tested the utility of liver homogenates from experimentally infected (YFV, Mayaro virus [MAYV], chikungunya virus [CHIKV], or mock) mice to quantify PRNTs.

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Kohmer, Niko, Cornelia Rühl, Sandra Ciesek, and Holger F. Rabenau. "Utility of Different Surrogate Enzyme-Linked Immunosorbent Assays (sELISAs) for Detection of SARS-CoV-2 Neutralizing Antibodies." Journal of Clinical Medicine 10, no. 10 (2021): 2128. http://dx.doi.org/10.3390/jcm10102128.

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The plaque reduction neutralization test (PRNT) is a preferred method for the detection of functional, SARS-CoV-2 specific neutralizing antibodies from serum samples. Alternatively, surrogate enzyme-linked immunosorbent assays (ELISAs) using ACE2 as the target structure for the detection of neutralization-competent antibodies have been developed. They are capable of high throughput, have a short turnaround time, and can be performed under standard laboratory safety conditions. However, there are very limited data on their clinical performance and how they compare to the PRNT. We evaluated thre

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Cosma, Antonio, Silja Bühler, Rashmi Nagaraj, et al. "Neutralization Assay Using a Modified Vaccinia Virus Ankara Vector Expressing the Green Fluorescent Protein Is a High-Throughput Method To Monitor the Humoral Immune Response against Vaccinia Virus." Clinical Diagnostic Laboratory Immunology 11, no. 2 (2004): 406–10. http://dx.doi.org/10.1128/cdli.11.2.406-410.2004.

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ABSTRACT Vaccination against smallpox is again considered in order to face a possible bioterrorist threat, but the nature and the level of the immune response needed to protect a person from smallpox after vaccination are not totally understood. Therefore, simple, rapid, and accurate assays to evaluate the immune response to vaccinia virus need to be developed. Neutralization assays are usually considered good predictors of vaccine efficacy and more informative with regard to protection than binding assays. Currently, the presence of neutralizing antibodies to vaccinia virus is measured using

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Terzian, Ana C. B., Sasha R. Azar, Cassia F. Estofolete, Mauricio L. Nogueira, and Nikos Vasilakis. "Differential Neutralization Profiles of 17DD Vaccinated Population to 17D-204 and 17DD Vaccine Strains." Vaccines 12, no. 12 (2024): 1311. http://dx.doi.org/10.3390/vaccines12121311.

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Background/Objectives: Yellow fever virus (YFV) (Flaviviridae, Orthoflavivirus) is the etiologic agent of yellow fever (YF), a vector-borne disease with significant morbidity and mortality across the tropics and neotropics, despite having a highly efficacious and safe vaccine (17D). Vaccination provides lifelong protection from YF disease mediated by humoral immunity. There are several versions of the original 17D vaccine: 17D-204 (marketed in the USA as YF-VAX, in France as Stamaril, and in China as Tiantan-V), 17D-213 (Russian Federation), and 17DD (by FIOCRUZ in Brazil). Vaccines produced i

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Li, Cui, Jianqing Wan, Deli Wang, et al. "Comparative Analysis of Hemagglutination Inhibition and Plaque Reduction Neutralization Tests for Japanese Encephalitis Virus Antibody Detection." Viruses 17, no. 1 (2025): 104. https://doi.org/10.3390/v17010104.

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Japanese encephalitis (JE) is a zoonotic disease caused by the Japanese encephalitis virus (JEV), belonging to the Flaviviridae family. Diagnosis of Japanese encephalitis (JE) based on clinical signs alone is challenging due to the high proportion of subclinical cases. The Plaque Reduction Neutralization Test (PRNT) is considered the gold standard for detecting JE-specific antibodies because of its high specificity. However, PRNT is complex, time-consuming, and requires live viruses, limiting its applicability in routine diagnostics. In this study, we compared the sensitivity and correlation o

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Ravault, Stéphanie, Damien Friel, Emmanuel Di Paolo, et al. "Assessment of Mumps Virus-Specific Antibodies: Comparison of Plaque Reduction Neutralization Test and Enzyme-Linked Immunosorbent Assay Estimates." Journal of Infectious Diseases 220, no. 9 (2019): 1462–68. http://dx.doi.org/10.1093/infdis/jiz345.

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Abstract Background The plaque reduction neutralization test (PRNT), which measures a subset of immunoglobulin antibodies (functional neutralizing antibodies), and the enzyme-linked immunosorbent assay (ELISA), which measures total immunoglobulin (neutralizing and nonneutralizing antibodies), characterize different aspects of the anti–mumps virus antibody response after vaccination. Methods Data from a recent phase 3 clinical trial (NCT01681992) of 2 measles-mumps-rubella vaccines were used to compare anti-mumps antibody responses measured using an unenhanced PRNT (GSK; seropositivity cutoff a

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Canakoglu, Nurettin, Engin Berber, Mustafa Ertek, et al. "Pseudo-plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus." Virology Journal 10, no. 1 (2013): 6. http://dx.doi.org/10.1186/1743-422x-10-6.

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Sarkale, Prasad, Anish Shrivastava, Sreelekshmy Mohandas, et al. "Growth Kinetics of Kyasanur Forest Disease Virus in Mammalian Cell Lines and Development of Plaque Reduction Neutralization Test." Vector-Borne and Zoonotic Diseases 19, no. 8 (2019): 630–36. http://dx.doi.org/10.1089/vbz.2018.2405.

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Phumiamorn, Supaporn, Skalin Trisiriwanich, Kornnika Kulabutar, et al. "Plaque reduction neutralization test as a method for detecting functional neutralizing antibodies against live SARS-CoV-2 virus." Thai Journal of Pharmaceutical Sciences 46, no. 3 (2022): 353–57. http://dx.doi.org/10.56808/3027-7922.2581.

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Nicolle, LE, A. Gutkin, G. Smart, et al. "Serological Studies of West Nile Virus in a Liver Transplant Population." Canadian Journal of Infectious Diseases and Medical Microbiology 15, no. 5 (2004): 271–74. http://dx.doi.org/10.1155/2004/606015.

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BACKGROUND: Solid organ transplant populations are at increased risk for serious clinical manifestations of West Nile virus (WNV) infection.OBJECTIVE: To monitor liver transplant recipients during the 2003 WNV season in Manitoba and to identify incidence, clinical presentation and serology.METHODS: Serial blood specimens were obtained from adult patients followed at the liver transplant outpatient clinic between May 2003 and October 2003. Studies for WNV infection included immunoglobulin (Ig) G and IgM enzyme immunoassay (EIA), hemagglutination inhibition (HI), plaque reduction neutralization

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