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Dissertations / Theses on the topic 'Electrochemistry of enzymes'

Author: Grafiati
Published: 4 June 2021
Last updated: 8 February 2022

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1

Whitaker, Richard George. "The electrochemistry of redox enzymes." Thesis, University of Warwick, 1989. http://wrap.warwick.ac.uk/4235/.

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The work presented in this thesis is of two types. Firstly methods for the electrochemical immobilisation of redox enzymes in organic polymers are described. The electrochemical monitoring of the immobilised enzyme reaction by detection of one of the enzyme's products is discussed, and the results obtained for such a system under a variety of experimental conditions are presented. A good understanding of the way in which such a system operates' was obtained by using a specially developed kinetic model., This model is explained fully in the theory chapter of this thesis. A variety of organic po
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2

Hunt, Nicholas Imber. "Biological electrochemistry." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386592.

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3

De, Oliveira Pedro M. A. "Studies of enzymes by electrochemistry and atomic force microscopy." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298717.

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4

Xu, Lang. "Investigating the current/voltage/power/stability capabilities of enzyme-based membrane-less hydrogen fuel cells." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:efef7124-3444-4531-872b-2ee8868e0aa0.

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Fuel cell is a device that can directly convert chemical energy into electrical energy. For low-temperature fuel cells, catalysts are required. Fuel cells using Pt-based or other non-biological materials as catalysts are known as conventional fuel cells. Inspired from Nature, enzymes can be used as catalysts in fuel cells known as enzyme-based fuel cells. The conventional and enzymatic fuel cells share the same underlying electrochemical principles, while enzyme-based fuel cells have their intrinsic advantages and disadvantages due to enzyme properties. The objective of this thesis is to inves
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5

Owens, Zachary J. "The purification and electrochemistry of his-tagged photosystem II." [Denver, Colo.] : Regis University, 2009. http://165.236.235.140/lib/ZOwens2009.pdf.

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6

Goldet, Gabrielle. "Electrochemical investigations of H2-producing enzymes." Thesis, University of Oxford, 2009. http://ora.ox.ac.uk/objects/uuid:696e5b9d-a80f-493e-85d4-0954be499b72.

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Hydrogenases are a family of enzyme that catalyses the bidirectional interconversion of H<sup>+</sup> and H<sub>2</sub>. There are two major classes of hydrogenases: the [NiFe(Se)]- and [FeFe]-hydrogenases. Both of these benefit from characteristics which would be advantageous to their use in technological devices for H<sub>2</sub> evolution and the generation of energy. These features are explored in detail in this thesis, with a particular emphasis placed on defining the conditions that limit the activity of hydrogenases when reducing H<sup>+</sup> to produce H<sub>2</sub>. Electrochemistry
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Yorke, Jake. "Engineering cytochrome P450BM3 into a drug metabolising enzyme." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:92dcddfe-b3fc-46e8-9e5e-77910fb03783.

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Directed evolution studies by Whitehouse et al. identified several variants of P450BM3 (CYP102A1) with enhanced substrate oxidation rates across a range of substrates. This thesis describes the use of these ‘generic accelerator’ variants, in combination with selectivity altering mutations to engineer P450BM3¬ for the oxidation of pharmaceuticals. Using engineered variants the non-steroidal anti-inflammatory drug diclofenac was metabolised to the primary human metabolites 4′- and 5-hydroxydiclofenac, with total conversion of 2 mM substrate by 5 μM enzyme. The local-anaesthetic lidocaine and the
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8

Jarrar, Haytem. "Bioélectrodes enzymatiques pour des applications en biocapteurs et en biopiles." Thesis, Montpellier, Ecole nationale supérieure de chimie, 2011. http://www.theses.fr/2011ENCM0017/document.

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La principale originalité de ce travail est la mise en œuvre de deux voies d'immobilisation du biorécepteur sur différents matériaux d'électrodes. Dans un premier temps, nous avons démontré que le polyneutral red (PNR) représente une bonne matrice de rétention pour les enzymes. De plus, de part ses propriétés de médiation vis-à-vis des enzymes et principalement de leur cofacteur (NAD/FAD), ce polymère permet une connexion intime entre le site actif de l'enzyme et l'électrode. L'ensemble de ces caractéristiques nous a permis de mettre en œuvre une bioélectrode applicable en tant qu'anode d'un b
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9

Wang, Vincent Cho-Chien. "New insights into enzymatic CO₂ reduction using protein film electrochemistry." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:f1061854-f6b8-4562-81e0-968c80e1da3a.

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Carbon monoxide dehydrogenase (CODH) is known to catalyze CO oxidation and CO₂ reduction reversibly with the minimal overpotential. A great advantage of protein film electrochemistry (PFE) is its ability to probe catalysis over a wide range of potentials, especially in the low potential region required for CO₂ reduction. CODH I and CODH II from Carboxydothermus hydrogenoformans(Ch) and the composite enzyme acetyl-CoA synthase/carbon monoxide dehydrogenase (ACS/CODH) from Moorella thermoacetica(Mt) are intensively studied throughout this thesis. The different catalytic redox-states in CODH, C<s
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10

Maerten, Clément. "Bio-inspired self-construction and self-assembly of organic films triggered by electrochemistry." Thesis, Strasbourg, 2016. http://www.theses.fr/2016STRAE045.

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Les architectures moléculaires qui se forment exclusivement sur une surface sont encore rares. L’électrodéposition est un procédé exploitant des « signaux » électriques afin de déclencher et contrôler l’assemblage de films. Récemment, une nouvelle méthode : l’autoconstruction de films en « une étape » par l’utilisation d’un morphogène (un gradient de catalyseur généré depuis une électrode), a attiré l’attention de la communauté scientifique. En effet, elle permet l’auto-assemblage rapide de films polymériques robustes. Cependant, cette technique était limitée à des systèmes basés sur la chimie
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11

Idris, Zulkifli. "Electrocatalytic cycling of nicotinamide cofactors by Ralstonia eutropha soluble hydrogenase." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:3d458a13-ce61-4ae4-bc93-5a7db3bb371d.

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Nicotinamide cofactors in their reduced and oxidised forms are important redox agents in biology. Of about 3000 dehydrogenases available to date, many require these cofactors for their activity. Dehydrogenases are of interest to chemists as they offer asymmetric catalysis to yield chiral products. The requirement of dehydrogenases for nicotinamide cofactors necessitates research into finding the best way of recycling the oxidised or reduced forms of these cofactors. Electrocatalytic NAD(P)H oxidation and NAD(P)⁺ reduction on standard electrodes is problematic due to unwanted side reactions and
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12

Hexter, Suzannah Victoria. "Principles of electrocatalysis by hydrogen activating metalloenzymes." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:60aeee02-a16c-4c86-bf48-61306512fa86.

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Hydrogenases catalyse the interconversion of H<sub>2</sub> and H<sup>+</sup>. Protein Film Electrochemistry (PFE), a technique in which a redox enzyme is adsorbed directly onto an electrode, enables a detailed description of the catalytic function of these metalloenzymes to be obtained. Unlike small-molecule electrocatalysts, the hydrogenase active site is surrounded by a protein structure ensuring that it is relatively unperturbed by the electrode surface. In this thesis, PFE is used alongside mathematical modelling to explain differences between [NiFe]- and [FeFe]-hydrogenases, highlighting s
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Roessler, Maxie M. "EPR investigations of iron-sulfur cluster relays in enzymes." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:ac6fa892-f54a-490d-927b-161231f00777.

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Electron paramagnetic resonance (EPR) spectroscopy is a powerful tool for obtaining structural information about chemical centres with unpaired electrons. In complex biological systems, EPR spectroscopy can be used to probe these paramagnetic centres and the long-range interactions between them. This thesis investigates two important types of enzymes, and in particular the role of the iron-sulfur electron-transfer centres they contain, with a variety of EPR techniques. Complex I (NADH:Ubiquinone Oxidoreductase) plays a key role in the electron transfer chain essential to the formation of ATP,
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14

Giroud, Fabien. "Biomatériaux d'électrode appliqués à la réalisation et à la caractérisation d'un biocapteur immunologique et de biopiles enzymatiques." Phd thesis, Université de Grenoble, 2011. http://tel.archives-ouvertes.fr/tel-00690367.

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Ce mémoire est consacré au développement d'un immunocapteur impédancemétrique et de deux biopiles enzymatiques. Premièrement, le poly(pyrrole-NHS) est utilisé pour l'immobilisation successive d'un modèle de la ciprofloxacine (CF) et de l'anticorps dirigé spécifiquement contre CF. La détection est réalisée par la spectroscopie d'impédance électrochimique. Elle détecte le déplacement en solution de l'anticorps. Le seuil de détection est de 1.10-12 g.mL-1. Deuxièmement, la production énergétique est abordée suivant deux approches. La première se base sur l'apparition d'un gradient de pH produit p
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15

Heath, Rachel Sarah. "Studies of a 'blue' copper oxidase electrocatalyst." Thesis, University of Oxford, 2008. http://ora.ox.ac.uk/objects/uuid:e8359408-d3d4-4fe3-910a-cc69265a1546.

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This thesis concerns the electrochemical investigation of high-potential laccases. These multicopper oxidases are efficient electrocatalysts for the dioxygen reduction reaction. A method for stabilising laccase on a graphite electrode was established. The method involved modification of the graphite surface by diazonium coupling of a 2-anthracene molecule. A laccase ‘film’ adsorbed on this modified surface remained stable for over two months and, typically, the current density for dioxygen reduction was doubled compared to a laccase ‘film’ on an unmodified surface. Protein film voltammetry was
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16

Lonsdale, Thomas. "Dihydrogen driven cofactor recycling for use in bio-catalysed asymmetric organic synthesis." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:a0407748-e34f-410a-9c78-a8316b7a3d4d.

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Asymmetric reductions are used to produce chiral molecules, which are important precursors for the pharmaceutical industry. Bio catalytic reductions often display high enantioselectivity without the cost and toxicity associated with metal catalysis. However, unlike metal catalysts which use H<sub>2</sub> directly, many useful redox-enzymes require the hydride donor NADH. NADH is expensive; therefore for a bio-catalytic process to be viable it must be recycled, usually by using a sacrificial carbon based substrate, generating super-stoichiometric amounts of waste. Two different methods for H<su
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17

Foster, Carina Elizabeth. "Reactions of [FeFe]-hydrogenase with carbon monoxide and formaldehyde." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:a1d43c4a-861a-4bb0-88ce-2edf4c334f79.

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The use of H2 as an energy carrier has in recent years been identified as a promising future solution to the current energy crisis. Hydrogenases are metalloenzymes found in many microorganisms and are used to catalyse the reversible inter-conversion of protons and H2. These enzymes and their synthetic analogues have been recognised as a way to facilitate the use of H2 as a fuel. A major challenge to the future use of these catalysts is their reactions with small molecule inhibitors, such as oxygen and carbon monoxide. Detailed understanding of the structure and catalytic mechanism of these hig
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18

Sabuncu, Sinan. "Investigation of enzymes from the respiratory chain by using electrochemical and spectroscopic techniques." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAF017/document.

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Le présent travail porte sur l’étude de deux protéines de la famille des oxydases à hème-fer par des techniques de spectroscopie et d’électrochimie. Le premier chapitre décrit l’étude du cytochrome bo3 oxydase issue d’E. coli. Nous nous sommes intéressés à l’étude des interactions enzyme-quinone par l’utilisation de quinones avec des longueurs chaines isoprenyl différentes. Notre but est de mieux comprendre le rôle de la longueur de la chaine des quinones sur l’activité catalytique de l’enzyme et sur les propriétés redox des cofacteurs à hème. Dans l’étape suivante, on a étudié les résidus imp
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19

De, poulpiquet de Brescanvel Anne. "Biopiles enzymatiques H2-O2 : nanostructuration de l'interface électrochimique pour l'immobilisation des enzymes redox." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4752/document.

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Dans la nature, la réduction de l'oxygène et l'oxydation de l'hydrogène sont catalysées par des enzymes oxydoréductases. Ces catalyseurs spécifiques, efficaces, renouvelables et biodégradables constituent une alternative séduisante au platine dans les piles à combustible. L'immobilisation à des interfaces nanostructurées de l'hydrogénase membranaire tolérante à l'oxygène de la bactérie hyperthermophile Aquifex aeolicus, et de la bilirubine oxydase thermostable de la bactérie Bacillus pumilus, a été étudiée dans ce sens.L'électrochimie et la dynamique moléculaire ont permis d'affiner le modèle
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20

Artigues, Cladera Margalida Esmeralda. "Estudio de biosensores electroquímicos basados en inmovilización enzimática." Doctoral thesis, Universitat Ramon Llull, 2019. http://hdl.handle.net/10803/667847.

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Els biosensors electroquímics són dispositius d'anàlisi que combinen l'especificitat de les reaccions bioquímiques amb la capacitat analítica de les tècniques electroquímiques. Gràcies a aquesta combinació, és possible determinar de forma ràpida, sensible i fiable diferents analits en mostres amb matrius complexes. Per tot això, l'ús de biosensors és una alternativa als mètodes clàssics d'anàlisi per a realitzar processos de control de qualitat en diferents sectors industrials. En la present Tesi, s'han desenvolupat biosensors amperomètrics enzimàtics basats en la immobilització d’oxidases so
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21

Jacques, Julien. "Réactivité de la nitrate réductase périplasmique étudiée par spectroscopie RPE et électrochimie directe." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4710/document.

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La nitrate réductase périplasmique de Rhodobacter sphaeroides catalyse la réduction du nitrate en nitrite. C'est une métalloenzyme qui comprend un cofacteur à molybdène, un centre fer - soufre et deux hèmes.La réactivité du cofacteur à molybdène reste mal comprise pour plusieurs raisons. Entre autres : l'hétérogénéité des signatures RPE Mo(V), état semi-réduit du site actif, et l'existence d'états inactifs de l'enzyme selon les conditions.Pour comprendre la réactivité et la pertinence catalytique des principales espèces Mo(V), nous avons entrepris une caractérisation des processus d'activation
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22

Reeve, Holly A. "New approaches for cofactor recycling : application to chemical synthesis and electrochemical devices." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:514ec071-36aa-45a9-9f03-15bb22f967c4.

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The work in this Thesis addresses the challenges associated with using redox enzymes for chemical synthesis. The use of enzymes as catalysts in the synthesis of fine chemicals is becoming more wide spread, in part due their ability to catalyse reactions with incredible selectivity under relatively mild conditions. In particular, enzymes are useful for selective reduction of ketones to enantiomerically pure alcohols or amines, and partial oxidations of alkanes to alcohols. However, a key limitation to exploiting redox enzymes in these reaction pathways is the requirement for a specialised elect
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23

Dochter, Alexandre. "Polymer films and brushes self-construction by electrochemically triggered morphogens." Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAE039/document.

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Les multicouches de polyelectrolytes, systèmes auto-assemblés par adsorption successive de polycations et de polyanions, constituent un matériau d’intérêt pour la fonctionnalisation de surface. Ce type de revêtement possède toutefois quelques limitations majeures : leur construction est lente et fastidieuse ; leur tenue mécanique et chimique est faible. Récemment, une méthode basée sur l’auto-construction de films par l’utilisation d’un morphogène, i.e. un gradient de catalyseur généré depuis une surface, a été développé permettant ainsi une rapide construction de revêtements robustes. Cette t
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24

Pescador, Álvarez Paula. "Colloidal and molecular assemblies for bioengineering applications." Doctoral thesis, Universitat Rovira i Virgili, 2007. http://hdl.handle.net/10803/8557.

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La técnica de recubrimiento "capa por capa' (layer-by-layer, LbL) de superficies cargadas mediante materiales con carga opuesta es una herramienta versátil para la fabricación de ensamblados moleculares e interfaces funcionales. Sus principales ventajas sobre otras metodologías, como las monocapas autoensambladas (SAM) o la técnica de Langmuir-Blodgett, son una enorme flexibilidad combinada con su gran simplicidad y bajo coste. Con instrumentación sencilla y protocolos de preparación simples, es posible ensamblar estructuras complejas y estables con un control nanométrico sobre su composición
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25

Woolerton, Thomas William. "Development of enzymatic H2 production and CO2 reduction systems." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:393741ac-94b1-4d56-b680-d9a434db77e2.

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One of today’s most pressing scientific challenges is the conception, development and deployment of renewable energy technologies that will meet the demands of a rapidly increasing population. The motivation is not only dwindling fossil fuel reserves, but also the necessary curtailment of emissions of the greenhouse gas carbon dioxide (a product of burning fossil fuels). The sun provides a vast amount of energy (120,000 TW globally), and one major challenge is the conversion of a fraction of this energy into chemical energy, thereby allowing it to be stored. Dihydrogen (H₂) that is produced fr
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Birkin, Peter Robert. "Microelectrochemical enzyme transistors." Thesis, University of Southampton, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240628.

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27

Siritanaratkul, Bhavin. "Enzyme-material composites for solar-driven reactions." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:55df8993-254b-4960-8ef4-fd9624206f3b.

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Using sunlight to drive chemical reactions has long been one of the goals in developing sustainable processes. Previous research has focused on solar fuel production in the form of H<sub>2</sub>, but this thesis demonstrates that solar-to-chemicals processes can be constructed to produce more complex compounds, using hybrid systems composed of enzymes and inorganic materials. Tetrachloroethene reductive dehalogenase (PceA), an enzyme that catalyzes the conversion of tetrachloroethene (PCE) to trichloroethene (TCE) and subsequently to cis-dichloroethene (cDCE), was shown to accept electrons fro
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28

Wulff, Philip. "Principles of hydrogen catalysis in the presence of oxygen by a [NiFe] hydrogenase from E. coli." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:9e434467-d50b-484a-a17e-ef3091636269.

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[NiFe] hydrogenases are metalloenzymes that act as highly efficient molecular electrocatalysts for the interconversion of protons and molecular hydrogen. Unlike any other known molecular electrocatalyst, the members of a subgroup of respiratory membrane-bound [NiFe] hydrogenases are able to maintain H<sub>2</sub> catalysis in the sustained presence of O<sub>2</sub>. This O<sub>2</sub>-tolerance depends on the ability to respond to oxidative inactivation by O<sub>2</sub> by exclusively forming rapidly reactivated active site states, thus implying a catalytic cycle in which O<sub>2</sub> acts as
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Singh, Kulveer. "Structure-function studies of the oxidoreductase bilirubin oxidase from Myrothecium verrucaria using an electrochemical quartz crystal microbalance with dissipation." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:0376cc7e-f572-4e0c-96f0-43b0b4b91d99.

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This thesis presents the development and redesign of a commercial electrochemical quartz crystal microbalance with dissipation (E–QCM–D). This was used to study factors affecting the efficiency of the four electron reduction catalysed by the fuel cell enzyme bilirubin oxidase from Myrothecium verrucaria immobilised on thiol modified gold surfaces. Within this thesis, the E–QCM–D was used to show that application of a constant potential to bilirubin oxidase adsorbed to thiol-modified gold surfaces causes activity loss that can be attributed to a change in structural arrangement. Varying the loa
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Keeley, Deborah Michelle. "Electrochemical studies of biologically important materials." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.325879.

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Mizzon, Giulia. "Bioelectrochemistry by fluorescent cyclic voltammetry." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:6a1134dd-c24d-4e60-ac83-936a6918131f.

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Understanding the factors influencing the ET characteristics of redox proteins confined at an electrochemical interface is of fundamental importance from both pure (fundamental science) and applied (biosensory) perspectives. This thesis reports on progress made in the emerging field of coupled electrochemical characterization and optical imaging in moving the analysis of redox-active films to molecular scales. More specifically the combination of cyclic voltammetry and wide-field Total Internal Reflection (TIRF) microscopy, here named ‘Fluorescent Cyclic Voltammetry’ (FCV), was applied to moni
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Mashazi, Philani Nkosinathi. "Study of metallophthalocyanines attached onto pre-modified gold surfaces." Thesis, Rhodes University, 2007. http://eprints.ru.ac.za/868/.

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Ho, Wah On. "Direct electron transfer peroxidase enzyme electrodes and their application to electrochemical immunoassay." Thesis, University of Newcastle Upon Tyne, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384829.

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Murphy, Bonnie J. "The importance of electron transfer in determining properties of [NiFe]-hydrogenases." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:42258640-0fa6-4c48-a0f6-d6c8b9d7b3e0.

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[NiFe] hydrogenases are microbial metalloenzymes that catalyse the reversible interconversion between molecular hydrogen and protons with high selectivity and efficiency. The catalytic properties of different [NiFe] hydrogenases vary according to the physiological roles they each play, yet all seem to be based upon an almost identical catalytic site architecture. Through efforts to understand the structural and mechanistic basis for the differing properties of [NiFe] hydrogenases, it has become increasingly evident that electron transfer to and from the active site, mediated by a set of Iron-S
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Mashazi, Philani Nkosinathi. "Electrochemical sensing and immunosensing using metallophthalocyanines and biomolecular modified surfaces." Thesis, Rhodes University, 2012. http://hdl.handle.net/10962/d1018248.

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The synthesis of cobalt and manganese phthalocyanine complexes bearing eight hexylthio and four amino substituents was carried out. The formation of thin films of these complexes using different modification methods was also studied. Hexylthio functionalized metallophthalocyanine complexes were immobilized onto gold electrode surfaces using the self-assembly techniques. Surface modifications using cobalt and manganese tetraamino phthalocyanine as polymers, monolayers (onto electrografted surfaces) and as carbon nanotube – metallophthalocyanine conjugates was also carried out. The new method of
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Paengnakorn, Pathinan. "Electrochemical and IR spectroelectrochemical studies of ligand binding to the metal centres of nitrogenase." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:83ea2ef0-c4a4-4014-8ba1-63ff11fbbbc4.

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Nitrogenase is a metalloenzyme that plays a key role in biological nitrogen fixation by catalysing the reduction of dinitrogen to ammonia. Study of nitrogenase is particularly challenging because of its unique electron transfer and catalytic components. This Thesis describes the development of a mediated electron transfer system for the MoFe protein of nitrogenase, in order to overcome the complexity of electron transfer by the native reductant Fe protein coupled to hydrolysis of ATP. A series of redox mediators was employed including Eu<sup>III/II</sup>-polyaminocarboxylate complexes, which h
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Petro, Benjamin J. "Preparation and Characterization of Hydrogenase Enzyme Active Site-inspired Catalysts: The Effects of Alkyl Bulk and Conformer Strain as Studied by Photoelectron Spectroscopy, Electrochemistry and Computational Methods." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/194329.

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A series of alkyldithiolatodiironhexacarbonyl complexes of the form &mu:-(RS2)Fe2(CO)6, where RS2 is: 1,2-ethanedithiolate (eth-cat), cis-1,2-cyclopentanedithiolate (pent-cat), cis-1,2-cyclohexanedithiolate (hex-cat), and 2-exo,3-exo-bicyclo[2.2.1]heptanedithiolate (norbor-cat), are reported. These complexes display structures and catalytic behavior toward production of molecular hydrogen with similarities to the active site of the diiron hydrogenase enzymes. Hydrogen production is desirable as an alternative fuel source and these catalysts are capable of producing H2 in the presence of weak a
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Song, Qingsheng. "Development of Dual Gas Diffusion-Type Biofuel Cells on the Basis of Electrochemical Understanding of Enzyme-Modified Electrodes." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225650.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第20425号<br>農博第2210号<br>新制||農||1047(附属図書館)<br>学位論文||H29||N5046(農学部図書室)<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授 加納 健司, 教授 宮川 恒, 教授 三芳 秀人<br>学位規則第4条第1項該当
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Kriegel, Sébastien. "Transformation of a membrane protein from the respiratory chain into a sensor for the analysis of its interaction with substrates, inhibitors and lipids." Phd thesis, Université de Strasbourg, 2013. http://tel.archives-ouvertes.fr/tel-01017392.

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The field of bioenergetics deals with the flow and transformation of energy within and between living organisms and their environment. The work presented in this thesis report focuses on cellular respiration and more specifically on the first enzyme of the respiratory chain, NADH:ubiquinone oxidoreductase (Complex I). This was done to clarify details about its function and its implication in disease. First, the creation of a sensor involving the biomimetically immobilized enzyme is presented and probed through a combination of surface enhanced infrared absorption spectroscopy (SEIRAS) and elec
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Hernández, Ibáñez Naiara. "Exploration of novel materials in (bio)electrocatalysis: sensing in complex media and biocathodes for the CO2 reduction." Doctoral thesis, Universidad de Alicante, 2018. http://hdl.handle.net/10045/88207.

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Las etapas de transferencia electrónica o transferencia de carga involucradas en reacciones electroquímicas juegan un papel muy importante en un gran número de procesos biológicos y bioquímicos. Hoy en día, el interés de la comunidad científica se centra en explorar y entender exhaustivamente la naturaleza biológica y química de los fenómenos bioelectroquímicos que ocurren en los seres vivos, con el objeto de mimetizarlos en el laboratorio. Los procesos bioelectrocatalíticos presentan un amplio abanico de aplicaciones dirigidas al: (i) desarrollo de biorreactores electroquímicos para la mitiga
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Tye, Jesse Wayne. "Explorations of iron-iron hydrogenase active site models by experiment and theory." [College Station, Tex. : Texas A&M University, 2006. http://hdl.handle.net/1969.1/ETD-TAMU-1014.

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42

Gerey, Bertrand. "Complexes homo- et hétéro-nucléaires de manganèse et de métaux alcalino-terreux : vers des modèles du centre de dégagement d'oxygène du photosystème II." Thesis, Université Grenoble Alpes (ComUE), 2015. http://www.theses.fr/2015GREAV041/document.

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Ce mémoire de thèse est consacré au développement et à la caractérisation de nouveaux complexes homo- et hétéronucléaires de manganèse et de métaux alcalino-terreux dans le cadre de la modélisation du cluster inorganique Mn4CaO5 de l’OEC du photosystème II.De nouveaux ligands incluant un nombre variable de groupements pyridine-carboxylates basés sur les architectures tris-(2-picolyl)amine et bis-(2-picolyl)ethylamine ont été synthétisés. Ces ligands ont permis d’isoler de nouveaux complexes homonucléaires avec Ca2+ et Sr2+ et ainsi que des complexes hétéronucléaires MnII–Ca. L’analyse par spec
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Esnault, Charles. "Modification électrochimique de surface pour la mesure des interactions ADN/Protéines (HsRad51 - Transposase)." Phd thesis, Université du Maine, 2012. http://tel.archives-ouvertes.fr/tel-00752894.

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Depuis l'apparition du terme "biosensor" à travers un article de Lyons et Clark en 1962, les biocapteurs ont connu un véritable essor tant au niveau académique qu'industriel. Le principal objectif de ce travail de thèse était de créer une surface permettant l'immobilisation spécifique par liaison covalente de simple ou double brin d'ADN puis d'étudier les interactions pouvant exister entre une protéine donnée et l'ADN. Pour préparer la surface à cette immobilisation, nous avons opéré une réduction électrochimique de sel d'aryldiazoniums. Ce type de modification nous a permis de fixer de manièr
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Nxusani, Ezo. "Synthesis and analysis of Novel Platinum group Metal Chalcogenide Metal Quantum Dot and Electrochemical Markers." University of the Western Cape, 2018. http://hdl.handle.net/11394/6424.

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Magister Scientiae - MSc (Chemistry)<br>Although cadmium and lead chalcogenide quantum dot have excellent optical and photoluminescent properties that are highly favorable for biological applications, there still exists increasing concerns due to the toxicity of these metals. We, therefore, report the synthesis of new aqueous soluble IrSe quantum dot at room temperature utilizing a bottom-up wet chemistry approach. NaHSe and H2IrCl6 were utilized as the Se and Ir source, respectively. High-resolution transmission electron microscopy reveals that the synthesized 3MPA-IrSe Qd are 3 nm in diamete
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"The Investigation and Characterization of Redox Enzymes Using Protein Film Electrochemistry." Doctoral diss., 2014. http://hdl.handle.net/2286/R.I.26871.

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abstract: Redox reactions are crucial to energy transduction in biology. Protein film electrochemistry (PFE) is a technique for studying redox proteins in which the protein is immobilized at an electrode surface so as to allow direct exchange of electrons. Establishing a direct electronic connection eliminates the need for redox­active mediators, thus allowing for interrogation of the redox protein of interest. PFE has proven a versatile tool that has been used to elucidate the properties of many technologically relevant redox proteins including hydrogenases, laccases, and glucose oxidase.
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Ortiz, Luis Angel. "High-throughput functional screening of oxidase enzymes." Thesis, 2020. https://hdl.handle.net/2144/42066.

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Our ability to sense small molecules with high specificity, over a broad range of concentrations, is limited and difficult to accomplish in a way that is inexpensive and continuous. The most commercially successful biosensor is the enzyme-based blood glucose electrochemical biosensor, yet for nearly all other biomolecules, detection and monitoring require specialized equipment, trained personnel, and long lead times, and are not amenable to continuous monitoring. Industries in need of enzyme-based small-molecule biosensors, including medical diagnostics, industrial production, environmental mo
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Leote, Ricardo José Branco. "Desenvolvimento e caracterização de biossensores eletroquímicos enzimáticos com enzimas oxidase." Master's thesis, 2017. http://hdl.handle.net/10316/83242.

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Dissertação de Mestrado em Química apresentada à Faculdade de Ciências e Tecnologia<br>Neste trabalho foram desenvolvidos biossensores enzimáticos a partir da modificação de eléctrodos de carbono vítreo com materiais nanoestruturados, nomeadamente nanotubos de carbono e mediadores redox com imobilização de enzimas oxidase. Esta modificação dos eléctrodos convencionais visa melhorar o desempenho dos biossensores aprimorando os parâmetros analíticos.O estudo está dividido em quatro capítulos.O primeiro capítulo trata aspectos gerais sobre a definição, classificação, e componentes dos biossensore
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Goran, Jacob Michael. "The bioelectrochemistry of enzymes and their cofactors at carbon nanotube and nitrogen-doped carbon nanotube electrodes." Thesis, 2014. http://hdl.handle.net/2152/30491.

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This dissertation explores the electrochemical behavior of enzymes and their cofactors at carbon nanotube (CNT) and nitrogen-doped carbon nanotube (N-CNT) electrodes. Two common types of oxidoreductases are considered: flavin adenine dinucleotide (FAD)-dependent oxidases and nicotinamide adenine dinucleotide-dependent (NAD⁺)-dehydrogenases. Chapter 1 presents the oxygen reduction reaction (ORR) at N-CNT electrodes as a way to electrochemically measure enzymatic turnover at the electrode surface. The unique peroxide pathway at N-CNT electrodes, which catalytically disproportionates hydrogen
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Maiocco, Stephanie Jane. "Biophysical characterization of electron transfer proteins containing multiple metallocofactors: investigation of the AdoMet radical and cytochrome c peroxidase enzyme superfamilies." Thesis, 2016. https://hdl.handle.net/2144/17706.

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Metallocofactors are ubiquitous in nature, serving multiple purposes in proteins. These metallocofactors typically act as the site of catalysis or as an electron relay to move electrons within the protein, or within the cell, and are very energetically costly to manufacture. Yet, in nature it can appear that supernumerary, or ‘auxiliary’ cofactors are apparent, with no clear function. In this thesis, I address the question of what roles additional cofactors play, and why they are retained. The radical S-adenosylmethionine (AdoMet) enzyme superfamily has displayed great diversity in the c
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