Relevant bibliographies by topics / Electrophoresis profile

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  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Electrophoresis profile'

Author: Grafiati
Published: 4 June 2021
Last updated: 4 February 2022

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Journal articles on the topic "Electrophoresis profile"

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Gwladys Ekwe Priso, Judith, Jean Pierre Nda Mefo’o, Cécile Okalla Ebongue, et al. "Electrophoretic Profile of Serum Proteins Using Capillary Technique in Patients Attending the Douala General Hospital, Cameroon." Avicenna Journal of Medical Biochemistry 6, no. 2 (2018): 50–55. http://dx.doi.org/10.15171/ajmb.2018.11.

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Background: Electrophoresis of serum proteins is an orientation examination routinely used in clinical practice. For a few years, agarose gel electrophoresis has tended to be replaced with capillary electrophoresis owing to an increase in the accuracy of results. However, this technique is uncommon and is not widely used in Cameroon. Objectives: The research aimed at studying the electrophoretic profile of serum proteins using capillary technique among patients attending the Douala General Hospital, Cameroon. Methods: Capillary electrophoresis was used to carry out tests on blood samples from any inpatients and outpatients and fasting for 8-12 hours. Capillary electrophoresis of serum samples was used for the separation of proteins into six fractions and the total protidemia of each serum samples was determined using the Biuret method. Results were interpreted by observing the shape of curves and quantitative variations in each fraction of the different serum proteins. Results: A total of 311 patients participated in the study. The sampled population aged 50±18 years on average and consisted of 55.3% men and 44.7% women. All capillary electrophoresis profiles presented six protein fractions, namely, albumin, alpha (1 and 2), beta (1 and 2) and gamma globulins. Pathological disorders were diagnosed in 290 patients and 21 patients had normal results. Inflammatory syndromes accounted for 63.34% and monoclonal gammopathies for 10.29% the main pathological disorder identified. Conclusion: Capillary electrophoresis provides a more precise identification of biological syndromes and clear distinction of the six fractions of each protein. Monoclonal profiles and inflammatory syndromes were well detected. A prevalence of 10.29% was determined for gammopathies.
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Santamaría, Mónica, Ángel M. Gutiérrez-Navarro, and Javier Corzo. "Lipopolysaccharide Profiles from Nodules as Markers of Bradyrhizobium Strains Nodulating Wild Legumes." Applied and Environmental Microbiology 64, no. 3 (1998): 902–6. http://dx.doi.org/10.1128/aem.64.3.902-906.1998.

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ABSTRACT To develop the use of electrophoretic lipopolysaccharide profiles for Bradyrhizobium strain identification, we studied the feasibility of using electrophoresis of whole legume nodule homogenates to obtain distinctive lipopolysaccharide profiles. The electrophoretic patterns were the same whether we used nodule extracts, bacteroids, or cultured bacteria as samples, and there was no evidence of changes in the ladder-like pattern during the nodulation process. To assess the reliability of using lipopolysaccharide profiling performed with individual nodules for studying the diversity and microdistribution of the rhizobia nodulating wild shrub legumes, we used a population ofAdenocarpus foliolosus seedlings. We obtained 75 different profiles from the 147 nodules studied. There was no dominant profile in the sample, and a plant with different nodules generally produced different profiles. Electrophoresis of legume root nodules proved to be a fast and discriminating technique for determining the diversity of a bradyrhizobial population, although it did not allow the genetic relationships among the nodulating strains to be studied.
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Mukhopadhyay, Rajendrani. "Research Profile: Electrophoresis in compact nanotube networks." Analytical Chemistry 78, no. 15 (2006): 5245. http://dx.doi.org/10.1021/ac069443x.

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Branchini, Maria Luiza Moretti, Débora de Cassia Pires Geiger, Olga Fischman, and Antonio Carlos Pignatari. "Molecular typing of Candida albicans strains isolated from nosocomial candidemia." Revista do Instituto de Medicina Tropical de São Paulo 37, no. 6 (1995): 483–87. http://dx.doi.org/10.1590/s0036-46651995000600002.

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Yeasts of the genus Candida have been recognized as important microorganisms responsible for nosocomial fungemia. Six blood-stream and two intravenous central catheter C. albicans strains were isolated from eight patients and studied by electrophoretic karyotyping of chromosomal DNA by pulsed-field gel electrophoresis. Seven chromosomal DNA profiles were identified. Two patients showed isolates with the same profile, suggesting nosocomial transmission. Karyotyping of C. albicans revealed an excellent discriminatory power among the isolates and may therefore be useful in the study of nosocomial candidemia.
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Palchevska, Snezhana, Velibor Tasik, Petar Korneti, Georgi Shestakov, and Svetlana Tsekovska. "Evaluation of urinary proteins in healty full-term neonates by SDS-PAGE." Macedonian Pharmaceutical Bulletin 51 (April 2005): 9–14. http://dx.doi.org/10.33320/maced.pharm.bull.2005.51.002.

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The pattern of urinary proteins in healthy full-term neonates was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), coupled with determination of few parameters related to urinary protein excretion. Twenty healthy full-term neonates were included in the investigation. Five urine samples from each subject were collected on days 3, 7, 14, 21 and 28 after birth. Determination of total proteins was performed using turbidimetric method with sulfosalicylic acid. Urinary creatinine concentration was determined by Jaffe method. Urinary proteins were separated by horizontal gradient SDS-PAGE according to Görg. The highest values for total urinary proteins and for protein/creatinine ratio were detected in urine samples excreted on days 3 and 7 after birth. Three types of SDS-PAG electrophoretic profiles were observed. The first type of electrophoretic profile was characterized by the presence of proteins of mixed glomerular and tubular origin with molecular weights from 10 to 160 kDa. Typical for the second type of electrophoretic profile was the presence of two faint fractions with molecular weights of 78 and 90 kDa and several intensive low molecular weight fractions (14-67 kDa). In the third type of electrophoretic profile only low molecular weight proteins (10-67 kDa) were detected in all five urine samples. These findings express the transitory immaturity of the glomerular filter and tubular protein reabsorbing system of the newborn kidney. Apparently, the tubular protein handling normalizes later than the glomerular filtration of proteins.
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Steele, M., B. McNab, L. Fruhner, S. DeGrandis, D. Woodward, and J. A. Odumeru. "Epidemiological Typing of Campylobacter Isolates from Meat Processing Plants by Pulsed-Field Gel Electrophoresis, Fatty Acid Profile Typing, Serotyping, and Biotyping." Applied and Environmental Microbiology 64, no. 7 (1998): 2346–49. http://dx.doi.org/10.1128/aem.64.7.2346-2349.1998.

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ABSTRACT Campylobacter spp. are a leading cause of bacterial gastroenteritis. Foods of animal origin, particularly undercooked poultry, are common sources ofCampylobacter species associated with disease in humans. A collection of 110 Campylobacter jejuni and 31 C. coli human and environmental isolates from different Ontario, Canada, abattoirs were analyzed by pulsed-field gel electrophoresis, fatty acid profile typing, and biotyping. Previously collected serotyping data for the same isolates were also analyzed in this study. Pulsed-field gel electrophoresis was found to be the most discriminatory of the typing methods, followed by serotyping, fatty acid profile typing, and biotyping. A wide variety of typing profiles were observed within the isolates, suggesting that several different Campylobacter sp. strains were present within the abattoirs.
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YOSHIDA, Akihiro, Michiteru KODAMA, Hideki NOMURA, and Michitaka NAITO. "Classification of Lipoprotein Profile by Polyacrylamide Gel Disc Electrophoresis." Internal Medicine 42, no. 3 (2003): 244–49. http://dx.doi.org/10.2169/internalmedicine.42.244.

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Kustos, Ildikó, Béla Kocsis, Ildikó Kerepesi, and Ferenc Kilár. "Protein profile characterization of bacterial lysates by capillary electrophoresis." Electrophoresis 19, no. 13 (1998): 2317–23. http://dx.doi.org/10.1002/elps.1150191311.

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Ne�elhut, T., W. Rath, G. Grospietsch, M. H. Weber, and W. Kuhn. "Urinary protein electrophoresis profile in normal and hypertensive pregnancies." Archives of Gynecology and Obstetrics 246, no. 2 (1989): 97–105. http://dx.doi.org/10.1007/bf00934126.

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Petrini, O., L. Toti, L. E. Petrini, and U. Heiniger. "Gremmeniella abietina and G. laricina in Europe: characterization and identification of isolates and laboratory strains by soluble protein electrophoresis." Canadian Journal of Botany 68, no. 12 (1990): 2629–35. http://dx.doi.org/10.1139/b90-332.

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Forty-four isolates of Gremmeniella abietina from different European regions and laboratory strains of G. abietina and Ascocalyx abietis, which originated from regenerated protoplasts, were characterized morphologically and by protein electrophoresis. On the basis of the electrophoretic profiles of all isolates tested, the North American race of G. abietina is not present in Europe. Isolates from Pinus cembra and two Finnish isolates differed slightly from the tester strain for the European race; their electromorphs were very similar to that of the European race on other hosts but differences were noticed that would support the maintenance of the variety cembrae. Gremmeniella abietina var. balsamea, common on Abies balsamea and Picea spp. in Quebec, Canada, seems to be absent from the European counterparts Abies alba and Picea abies. The isolates from P. abies belong unequivocally to the European race of G. abietina. European isolates of G. laricina were morphologically and electrophoretically indistinguishable from the North American ones. The P. cembra and Larix decidua endophytes showed the same electrophoretic profile as the isolates from diseased pines identified as Brunchorstia pinea var. cembrae. No changes in the electrophoretic patterns of laboratory strains derived from regenerated protoplasts, compared with those of the original isolates, were observed. Two isolates from Abies do not belong to any known species of Brunchorstia; on the basis of morphological, electrophoretic, and immunological evidence they belong to a taxon taxonomically close to G. abietina. Key words: Gremmeniella abietina, protein electrophoresis, Pinus cembra, Picea abies, European isolates.
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Dissertations / Theses on the topic "Electrophoresis profile"

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Malmberg, Jennie. "The neuroanatomical expression profile of novel membrane proteins. : The effect of macronutrients on gene expression." Thesis, Mälardalen University, School of Sustainable Development of Society and Technology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-1105.

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<p>Worldwide obesity is an increasing problem. Apart from the fact that obesity greatly  impairs the health, quality and length of life for the affected individuals, it is also has the  potential to become a major socioeconomic problem in a near future. However preventive  actions require an understanding of the cause. Before the psychological influence on  eating can be evaluated a profound understanding of the biological regulatory system and  how this interacts with the food consumed is required. On the assumption that food  consumption is regulated by interplay between food and genes, the food itself may  influence the genes that regulate consumption, hence change the expression levels of the  genes regulating food intake.     To evaluate the interplay between food and gene expression, the project contained several  parts, reflecting different aspects of the area of research. The feeding studies had in  common that they were initial trials in a larger project. The results of these will be  evaluated and used in combination with further studies.     The mice typed for food preference illustrate the complexity of the feeding regulatory  system by pointing out the differences between individuals even in a relatively small  group of animals. Mice in general like food high in fat and here the animals that showed a  preference for sugar also showed a significant increase in their intake of chow. Since  chow consists mainly of carbohydrates the results might indicate a preference not for  sucrose in particular but for carbohydrates in general. The effect this may have on other  studies is still unclear as further studies are needed to determine whether the difference  may be the result of an innate genetic difference.      Leucine has been previously shown to reduce the total caloric intake. When given in  combination with palatable food the addition of Leucine primarily reduced the intake of  chow. From a dietary perspective this would translate to a preference to sweets and fast  food at the expense of food with more nutritious content.     The RT-PCR analysis’s gives clues to how the energy regulatory circuitry responds to the  intake of selected macronutrients. When it comes to gene expression there is a significant  effect of macronutrients on the gene expression levels. The common theme for many of  the genes tested seems to be down regulation of satiety signals, as if to support over  feeding on palatable diets and in many cases sucrose in particular.     The intake of macronutrients such as sugar or fat has been showed to have an effect on  the feeding regulatory circuitry, demonstrated by the change in gene expression levels.   The response to said macronutrients is site specific which is clearly shown both by RTPCR analysis of samples from different parts of the brain, such as the brainstem or  hypothalamus, and by immunohistochemistry of selected areas. The  immunohistochemistry also confirms that the novel Oxytocin receptor-antagonist, who is  injected IP, actually passes over the blood-brain barrier and has an actual affect on the  regions of interest. The areas affected by the antagonist can be visualized and identified  through the staining of active sites.</p>
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Pinto, Thiago Matos. "Modelagem do potencial elétrico através da membrana do neurônio ganglionar e células de neuroblastoma: efeitos das cargas superficiais." Universidade do Estado do Rio de Janeiro, 2010. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2417.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>O objetivo do presente trabalho é comparar, do ponto de vista elétrico, a membrana do neurônio ganglionar com a da célula de neuroblastoma, analisando os efeitos das cargas fixas sobre o potencial elétrico nas superfícies da bicamada lipídica e também sobre o comportamento do perfil de potencial através da membrana, considerando as condiçõesfísico-químicas do estado de repouso e do estado de potencial de ação. As condições para a ocorrência dos referidos estados foram baseadas em valores numéricos de parâmetros elétricos e químicos, característicos dessas células, obtidos na literatura. O neurônio ganglionar exemplifica um neurônio sadio, e a célula de neuroblastoma, que é uma célula tumoral, exemplifica um neurônio patológico, alterado por esta condição. O neuroblastoma é um tumor que se origina das células da crista neural (neuroblastos), que é uma estrutura embrionária que dá origem a muitas partes do sistema nervoso, podendo surgir em diversos locais do organismo, desde a região do crânio até a área mais inferior da coluna. O modelo adotado para simular a membrana de neurônio inclui: (a) as distribuições espaciais de cargas elétricas fixas no glicocálix e na rede de proteínas citoplasmáticas; (b) as distribuições de cargas na solução eletrolítica dos meios externo e interno; e (c) as cargas superficiais da bicamada lipídica. Os resultados que obtivemos mostraram que, nos estados de repouso e de ação, os potenciais superficiais da bicamada interno (ÁSbc) e externo (ÁSgb) da célula de neuroblastoma não sofrem alteração mensurável, quando a densidade de carga na superfície interna (QSbc) torna-se 50 vezes mais negativa, tanto para uma densidade de carga na superfície externa da bicamada nula (QSgb = 0), como para um valor de QSgb 6= 0. Porém, no estado de repouso, uma leve queda em ÁSbc do neur^onio ganglionar pode ser observada com este nível de variação de carga, sendo que ÁSgb do neurônio ganglionar é mais negativo quando QSgb = 1=1100 e/A2. No estado de ação, para QSgb = 0, o aumento da negatividade de QSbc não provoca alteração detectável de ÁSbc e ÁSgb para os dois neurônios. Quando consideramos QSgb = 1=1100 e/A2, ÁSgb do neurônio ganglionar se torna mais negativo, não se observando variações detectáveis nos potenciais superficiais da célula de neuroblastoma. Tanto no repouso quanto no estado de ação, ÁSgb das duas células não sofre variação sensível com o aumento da negatividade da carga fixa distribuída espacialmente no citoplasma. Já a ÁSbc sofre uma queda gradativa nos dois tipos celulares; porém, no estado de ação, esta queda é mais rápida. Descobrimos diferenças importantes nos perfis de potencial das duas células, especialmente na região do glicocálix.<br>The aim of our work is to compare, from the electrical point of view, the ganglion neuron membrane with the neuroblastoma cell's membrane, analyzing the effects of fixed charges on the electric potential of the surfaces of the lipidic bilayer and on the behavior of the potential profile across the membrane, considering the physicochemical conditions of the resting state and of the action potential state. The conditions for the occurrence of these states were defined, based on numerical values of electrical and chemical parameters of these cells, obtained in the literature. The ganglion neuron portrays a healthy neuron,and the neuroblastoma cell, which is a tumor cell, represents a pathologic neuron, different from the ganglion cell, due to this condition. A neuroblastoma is a tumor, originated from neural crest cells (neuroblasts), which is an embryonic structure that gives rise to many parts of the nervous system and can arise in various body sites, from the region of the skull all the way to the lower spinal column area.The model used to simulate the neuron membrane includes: (a) the spatial distribution of the fixed electric charges on the glycocalyx and on the network of cytoplasmic proteins; (b) the distribution of the charges in the electrolytic solution of outer and inner resources; and (c) the surface charges of the lipidic bilayer. The results we obtained show that, in the resting and action states, the inner (ÁSbc) and outer (ÁSgb) surface potential of neuroblastoma cells do not change measurably, when the charge density on the inner surface (QSbc) becomes 50 times more negative, for both null charge density on the outer surface (QSgb = 0) and for QSgb 6= 0. However, a slight drop in ÁSbc of a ganglion neuron can be observed with this level of charge variation, but ÁSgb of ganglion neuron is more negative when QSgb = 1=1100 e/A2. At action potential state, for QSgb = 0, the negative increase of QSbc does not measurably change ÁSbc and ÁSgb , for both neurons. When we consider QSgb = 1=1100 e/A2, for the ganglion neuron ÁSgb becomes more negative, with no significant detectable changes in the neuroblastoma cell's surface potentials. At the resting and action states, ÁSgb of both cells does not undergo substantial changes with the negative increasing of fixed charges uniformly distributed in the cytoplasm. However,ÁSbc undergoes a gradual decrease in both cell types, although for the action state, this fall is faster. We discovered important dierences among the potential profile of the two cells, especially in the glicocalyx region.
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Weckel, Alexis. "Characterization of Pressure-Driven and Electro-Kinetically Driven Flow in a Micro-Fluidic Chip Using Particle Imaging Velocimetry." DigitalCommons@CalPoly, 2015. https://digitalcommons.calpoly.edu/theses/1393.

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The flow profiles of pressure-driven and electro-kinetic driven flows were compared for a microfluidic chip. It was found that the pressure-driven flow had a parabolic profile while the electro-kinetic flow had a plug shaped flow profile. The measured velocities were similar to those determined by the Poiseuille flow model and the Helmholtz-Smoltchowski equation. Flow uniformity is very important for control in microfluidic mixers. Parabolic flow profiles lead to inconsistent reactions while the more uniform plug shape flow allow for a more steady reaction across the channel. Previous work had been performed to measure the flow of a solution of fluorescent polystyrene beads in PDMS channels using a laser confocal microscope. This showed that particles easily stuck to the channel making it difficult to measure over time. In addition, bubble formation in the channel made measuring velocities difficult. Current work used a LabSmith Video Synchronized microscope with software to measure the flow rates at different areas of the channel. Solutions of fluorescent polystyrene beads were used to visually observe the flow within a channel under a microscope. Four different channels were used for the pressure-driven flows of varying dimensions and materials. The channel with the best measured profile was also measured under electro-kinetic flow. A LabSmith High Voltage Sequencer was used to apply a voltage across the channel for electro-kinetic measurements. This research confirmed the different flow profiles under pressure-driven and electro-kinetic driven flow. Future work can be done to determine how this effects mixing in the channels.
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Maciel, Roberto Marinho. "PERFIL ELETROFORÉTICO DAS PROTEÍNAS SÉRICAS DE FRANGOS DE CORTE ALIMENTADOS COM DIETAS CONTENDO AFLATOXINAS E/OU ARGILA CLINOPTILOLITA NATURAL." Universidade Federal de Santa Maria, 2006. http://repositorio.ufsm.br/handle/1/10023.

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Aflatoxins are metabolites from fungi, that may be injurious to animal health, resulting in reduced production and affecting poultry industry by decrease the growth rate and worsening feed conversion. A study was carried out to evaluate the electrophoresis profile of serum protein in broilers fed with diets containing aflatoxins and natural clinoptilolite clay. A total of 528 male broilers Ross were used, distributed in 6 treatments with 4 replications each: T1 control (without aflatoxins or clinoptilolite), T2 5ppm of aflatoxins, T3 0.25% of clinoptilolite, T4 5ppm of aflatoxins and 0.25% of clinoptilolite, T5 0.5% of clinoptilolite and T6 5ppm of aflatoxins and 0.5% of clinoptilolite. The broilers were placed in 24 boxes and submitted to treatments from 1 to 42 days, when they were slaughtered. Were analyzed the total proteins, albumin fractions, alpha 1, alpha 2, beta and gamma. The aflatoxins decreased (P<0.05) total protein, albumin, and globulins (alpha and beta fractions). However, no effect (P<0.05) of aflatoxins was observed about gamma fraction. The clinoptilolite no modify (P<0.05) the serum proteins. Related to control, broilers fed with diets containing aflatoxins and clinoptilolite presented low (P<0.05) levels of total protein, albumin, and globulins (alpha and beta fractions). In conclusion, aflatoxins change the electrophoresis profile and clinoptilolite is not able to protect against this change<br>Aflatoxinas são metabólitos de fungos, que podem causar danos à saúde do animal, resultando em redução da produção e afetando à indústria avícola, pela diminuição da taxa de crescimento e péssima conversão alimentar. O objetivo do presente trabalho foi avaliar o perfil eletroforético das proteínas séricas de frangos de corte alimentados com dietas contendo aflatoxinas e/ou argila clinoptilolita natural. Foram utilizados 528 frangos de corte, machos, da linhagem Ross, distribuídos em 6 tratamentos com 4 repetições cada: T1 testemunha (ração sem aflatoxinas ou clinoptilolita), T2 ração com 5ppm de aflatoxinas, T3 ração com 0,25% de clinoptilolita, T4 ração com 5ppm de aflatoxinas e 0,25% de clinoptilolita, T5 ração com 0,5% de clinoptilolita e T6 ração com 5ppm de aflatoxinas e 0,5% de clinoptilolita. Os animais ficaram alojados em 24 boxes, e submetidos aos tratamentos do 1º ao 42º dia, quando foram sacrificados. Foram analisadas as proteínas totais, as frações albumina, alfa 1, alfa 2, beta e gama. Com exceção das médias da fração gama, o teste de Tukey revelou diferenças significativas (P<0,05) nas médias de todas as proteínas totais e frações protéicas nos tratamentos onde as aflatoxinas estavam presentes. A ação das aflatoxinas nas proteínas totais ocorre na síntese de albumina e globulinas (frações alfa e beta). As gamaglobulinas não são afetadas pelas aflatoxinas. Em relação ao controle, as aves alimentadas com dietas com aflatoxinas e clinoptilolita apresentaram baixos (P<0,05) níveis de proteína total, albumina e globulinas (alfa e beta). Conclui-se que as aflatoxinas alteram o perfil eletroforético e a clinoptilolita adicionada na ração, não é capaz de evitar essa alteração
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Šopíková, Martina. "Změny proteinového profilu v průběhu sladování ječmene." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216437.

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This diploma thesis is focused on studies of changing of protein profile during barley malting. Substantial part of this work is devoted to the proteomics identification of barley proteins which change during malting and so become more stationary and they influence quality of beer (haze and foam in beer). For this experiment was used barley variety Jersey. In the theoretical part of this thesis there is information about beer, manufacturing of beer with description of important commodities for manufacturing of beer and information about barley malting and information about malting process. Next there is description of methods for separation of proteins (1D gel electrophoresis and 2D gel electrophoresis), MALDI TOF/TOF mass spectrometry and this use for the analysis and identification of proteins, the use of matrices and ways of the sample preparation. In the experimental part of this thesis there was carried out the optimisation of the dosage of sample for 1D gel electrophoresis and the optimisation of staining. The 15 % TRIS-HCl gel was the best, this gel was stained by Commassie Brilliant Blue G-250. For illustration of changes was made 2D gel electrophoresis. With help of method peptide mass fingerprinting and MS/MS protein of barley – protein Z, -amylase subtilisin inhibitor, -amylase a peroxidase were identificated. The analysis of barley extract intact proteins was carried out, this analysis was focused on changes of important barley protein LTP 1.
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VALTAT, BRUNO. "Etude electrophoretique du sperme humain coagule et liquefie : profils obtenus sur gel de polyacrylamide en tampon dissociant-discontinu." Strasbourg 1, 1990. http://www.theses.fr/1990STR15035.

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Anduri, Sridevi. "Differential protein expression profiles in normal and intersex male smallmouth bass determined using one- and two-dimensional gel electrophoresis a thesis presented to the faculty of the Graduate School, Tennessee Technological University /." Click to access online, 2009. http://proquest.umi.com/pqdweb?index=0&did=2000377671&SrchMode=1&sid=2&Fmt=6&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1277817194&clientId=28564.

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DELIE, PRIEUR ANNE. "Profils en page-sds des proteines de membrane externe et des proteines solubles du contenu cellulaire de 14 souches de bilophila wadsworthia." Lille 2, 1993. http://www.theses.fr/1993LIL2P029.

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"PROFILE PLASMATIC ELECTROPHORESIS IN RHEAS (Rhea americana) OF DIFFERENT AGE GROUPS." Tese, Biblioteca Digital de Teses e Dissertações da UFSM, 2005. http://coralx.ufsm.br/tede/tde_busca/arquivo.php?codArquivo=838.

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Cheng, Chao-Ying, and 鄭朝營. "Outer membrane protein profile in ceftriaxone resistant Salmonella typhimurium by two-dimensional gel electrophoresis analysis." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/62388549257183941157.

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碩士<br>國立陽明大學<br>醫學生物技術研究所<br>91<br>Abstract Ten clinical isolates of Salmonella enterica serovar Typhimurium were investigated for antimicrobial susceptibility by disk diffusion test. All ten isolates are resistant to ampicillin, streptomycin, chloramphenicol and tetracycline. These four antibiotic resistance are commonly found in mutildrug resistant strains of Salmonella serovar Typhimurium. Also, we found that a tendency towards the development of cephalosporin resistance. To explore the mechanism of resistant to cephalosporin, in this study, we developed a series of ceftriaxone(CRO) resistant strains of Salmonella serovar Typhimurium using in vitro multi-step drug induction method. The minimum inhibitory concentration (MIC) value of those resistant strains were from 0.05 μg/ml to 256 μg/ml which determined by E test. We excluded the possibility of antibiotic resistance mechanism of the drug inactivation by β-lactamases. To obtain more detail information about outer membrane proteins involving in CRO resistance, we used a modified two-dimensional gel electrophoresis(2-DE) technique to analyze the profile of Salmonella serovar Typhimurium outer membrane proteins between 01-4 wild type and different MIC value of CRO resistant strains. The density of several protein spots showed either increasing or decreasing in 2-DE gel. Among them, there are seven protein spots significantly reduced expression, however, eight protein spots significantly increased expression. By using mass spectrometry (MALDI-TOF or LC/MS/MS), we identified six of seven reduced protein, OmpF, OmpC, PhoE, AcrE, OmpW(YciD) and FdoH, and one of eight overexpressed protein, STM3031, whose function has been unclear, but belongs to virulence-related outer membrane protein family. These outer membrane proteins may play important roles in the development of CRO resistance in Salmonella serovar Typhimurium, and are necessary for further study in the near future.
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Books on the topic "Electrophoresis profile"

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Graham, Robert Noel. The evaluation of a method for the epidemiological typing of coagulase-negative staphylococci by combining biotyping, antibiograms and esterase electrophoresis profiles. The Author], 1992.

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Book chapters on the topic "Electrophoresis profile"

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Sizova, Daria. "Protein Expression Profile of Alzheimer’s Disease Mouse Model Generated by Difference Gel Electrophoresis (DIGE) Approach." In Genomics, Proteomics, and the Nervous System. Springer New York, 2010. http://dx.doi.org/10.1007/978-1-4419-7197-5_19.

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Kinoshita, Mitsuhiro, and Kazuaki Kakehi. "Capillary Electrophoresis Capillary electrophoresis of Glycans: Validation of Antibody Pharmaceuticals Based on Glycan Profiles." In Glycoscience: Biology and Medicine. Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-54841-6_9.

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Luque, Juan Antonio. "Interpretation Guidelines for Mixed-STR Multilocus Electrophoretic Profiles." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-461-2_15.

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Maramis, C. F., and A. N. Delopoulos. "Improved Modeling of Lane Intensity Profiles on Gel Electrophoresis Images." In XII Mediterranean Conference on Medical and Biological Engineering and Computing 2010. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-13039-7_169.

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Silva-Mata, Francisco, Isneri Talavera-Bustamante, Ricardo González-Gazapo, Noslén Hernández-González, Juan R. Palau-Infante, and Marta Santiesteban-Vidal. "Automatic Extraction of DNA Profiles in Polyacrilamide Gel Electrophoresis Images." In Lecture Notes in Computer Science. Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/11578079_26.

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Sen, Dipankar. "Whole-Cell Protein Profiles of Soil Bacteria by Gel Electrophoresis." In SSSA Book Series. Soil Science Society of America, 2018. http://dx.doi.org/10.2136/sssabookser5.2.c29.

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Kinoshita, Mitsuhiro, and Kazuaki Kakehi. "Capillary Electrophoresis of Glycans: Validation of Antibody Pharmaceuticals Based on Glycan Profiles." In Glycoscience: Biology and Medicine. Springer Japan, 2014. http://dx.doi.org/10.1007/978-4-431-54836-2_9-1.

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Anne, G., J. Vleugels, and O. Van der Biest. "Engineering the Composition Profile in Functionally Graded Materials Processed by Electrophoretic Deposition." In Ceramic Transactions Series. John Wiley & Sons, Inc., 2012. http://dx.doi.org/10.1002/9781118408391.ch5.

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Somasegaran, Padma, and Heinz J. Hoben. "Analyzing Plasmid Profiles of Rhizobium spp. by a Modified Eckhardt Vertical Gel Electrophoresis Procedure." In Handbook for Rhizobia. Springer New York, 1994. http://dx.doi.org/10.1007/978-1-4613-8375-8_30.

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"2D-DIGE A Powerful Tool for Proteome Analysis." In Protocols used in Molecular Biology, edited by Sudhir K. Shekhar, Jai Godheja, and Dinesh Raj Modi. BENTHAM SCIENCE PUBLISHERS, 2020. http://dx.doi.org/10.2174/9789811439315120010010.

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In the recent past, two dimensional gel electrophoresis has emerged as a powerful molecular biology tool for the comparative expression profiling of complex protein sample. It involves the separation as well as the resolution of diverse proteins sample on the basis of isoelectric points and molecular mass of protein in two dimension ways. In this way, it reflects the view of overall proteome status including differentiation in protein expression levels, post-translational modifications etc. Moreover, this allows the identification of novel biological signatures, which may give a particular identity of pathological background to cells or tissues associated with various types of cancers and neurological disorders. Therefore, by utilizing such tools, one can clearly investigate and compare the effects of particular drugs on cells of tissues and also one can analyze the effects of disease on the basis of variations in protein expression profile at broad spectrum. Recently, to get more error-less and accurate proteome profile, conventional 2-D gel electrophoresis has been enhanced with the inclusion of different types of protein labeling dyes which enables a more comparative analysis of diverse protein sample in a single 2-D gel. In this advanced technique (2-D-DIGE), protein samples are labeled with three different types of CyDyes (Cy2, Cy3, and Cy5) separately and combined and further resolved on the same gel. This will facilitate the more accurate spot matching on a single gel platform and will also minimize the experimental variations as commonly reported in the conventional 2D-gel electrophoresis. Therefore, in the present proteomic research era, 2D-DIGE has proved to be an extremely powerful tool with great sensitivity for up to 125 ng of proteins in clinical research volubility especially, neurological and cancer related disorders.
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Conference papers on the topic "Electrophoresis profile"

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Crook, M., S. J. Machin, and N. Crawford. "PLATELET SUBPOPULATION HETEROGENEITY IN IDIOPATHIC THROMBO-CYTOPAENIA AND ESSENTIAL THROMBOCYTHAEMIA STUDIED BY CONTINUOUS FLOW ELECTROPHORESIS: MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644576.

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Using continuous flow electrophoresis (CFE) normal human platelets have been separated into a surface-charge dependent profile (roughly gaussian) extending over 15-20 fractions with an electrophoretic mobility range between 0.86 and 0.93 μmetres/sec Volt cm. The technique has also been used to study platelets from adult patients with Stable Essential Thrombocythaemia (platelet counts - 600-800 × 109/L) and chronic Idiopathic Thrombocytopaenia (counts 15-80 × l09/L). Neither group was receiving specific therapy during the investigations. The platelets from both patient groups showed significant anodal shifts in electrophoretic mobilities in comparison with the CFE profiles from normal adults. Sialic acid, a negatively charged sugar moiety, associated with membrane glycoproteins is a major contributor to the platelet surface electronegativity and both total platelet sialic acid and surface neuraminidase-labile sialic acid measurements were made using the thiobarbituric acid procedure. Platelets from the Idiopathic Thrombocytopaenic group showed (with respect to normal levels) significant increases in both total and neuraminidase-labile sialic acid (expressed in terms of platelet number). Platelets from the Essential Thrombocythaemic group did not differ significantly in sialic acid contents from platelets from normal subjects. Platelet sizes (Coulter counter volumes) were also measured across the normal and patient platelet electrophoretic profiles together with morphological monitoring. In platelets from ITP patients these more electronegative platelets were substantially larger and of more bizarre shapes than those of normal patients when viewed with scanning and transmission electron microscopy, whereas platelets from the Essential Thrombocythaemic group showed less significant volume increases. These findings of marked changes in platelet surface electrokinetic and analytical properties in patients with the two disorders suggest that studies in other disease states, involving disturbances of thrombokinetics, may be warranted and particularly should be followed through therapeutic programmes.
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Buko, Alexander, Leo Cheng, Andrew Gustev, and Adam Feldman. "Abstract 3510: The metabolomic profile of urine from prostate cancer patients using capillary electrophoresis mass spectrometry (CEMS)." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-3510.

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Crook, M., and N. Crawford. "A STUDY OF HUMAN PLATELET SUBPOPULATION HETEROGENEITY IN FRACTIONS SEPARATED BY CONTINUOUS FLOW ELECTROPHORESIS (CFE)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643903.

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Earlier density gradient studies showed that the circulating platelet pool has substantial heterogeneity with respect to size, buoyant density and metabolic and functional properties. The interpretation of this heterogeneity is, however, controversial; some workers attributing it to in vivo ageing processes, whilst others consider it relates more closely to the megakaryocyte ploidy classes at the time of platelet production. Such considerations are important for further understanding of disease states where the heterogeneity profile may be distorted by the presence of megathrombocytes or other atypical platelet forms due to changes in the thrombopoeitic equilibrium (e.g. Idiopathic or drug-induced thrombocytopaenias, splenomegaly and leukaemia etc). We describe a new procedure for investigating platelet heterogeneity using a continuous flow method to isolate subpopulations on the basis of differences in surface membrane electrokinetic properties. To maintain the cells quiescent during the isolation and CFE they have been pre-treated with taxol, a microtubule stabilising agent. Taxol maintains platelet discoidicity, yet the cells show full functional responses to conventional agonists. The heterogeneity profile emerging from the CFE chamber extends over 15-20 fractions and spans a range of electrophoretic mobility (measured with a Laser Doppler Analytical apparatus) between 0.83-0.93 μmetres/sec/V. cm. Pooled fractions from the profile show differences in total and surface neuraminidase-labile sialic acid which correlate well with their electrokinetic properties. Interestingly, DTNB-titratable -SH groups in surface membrane proteins show an inverse relationship to platelet electronegativity as do phosphate-labile phosphate groups. There are significant differences in platelet size (Coulter volume) with the larger platelets being more electronegative in terms of unit charge density than the smaller ones. This novel procedure for studying platelet subpopulations on the basis of surface electrokinetic properties may also have value in profiling platelets from patients with specific platelet defects, during extracorporeal circuitry, transplant rejection and in vessel wall diseases.
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Jomeh, Sina, and Mina Hoorfar. "Study of the Effect of Electrophoresis on Transport of Biomolecules in Microreactors." In ASME 2011 9th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2011. http://dx.doi.org/10.1115/icnmm2011-58165.

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The effect of electrophoresis (i.e., applying uniform electric field to use the natural charge of particles) on the transport of a sample (like biomolecules) in active microreactors is numerically investigated. Navier-Stokes equations are solved along with the equations of electrostatics, species mass transport in the buffer and chemical reaction kinetics at reactive surfaces. Unlike previous studies, in which the effect of the charge of the sample bulk on the electric field has been neglected (i.e., the assumption of electroneutrality), here space charge density is assumed to be nonzero. As a result, the governing equations become fully coupled. The efficiency of the microreactor device is analyzed for two different geometries commonly used in biomolecule separation (i.e., open channel and microcylinders). It is shown that the electroneutrality assumption can drastically influence the final adsorbed concentration depending on the device configuration. Average adsorbed surface concentration is compared for each case as a measure of the performance of the device. The plots depicting the influence of the electric field and nonzero space charge density on the bulk concentration profile and the velocity field are also presented and discussed.
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Yang, Lijuan, Mengchun Gao, Fangyuan Liang, and Chao Qin. "Microbial Community Analysis During Composting of Digested Sludge and Sawdust by Using Quinone Profile and Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5163049.

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Kannan, Surya, and Serhiy Souchelnytski. "Post-Translational Modifications of Albumin in Cancer – A Rich Source for Diagnostic and Monitoring of Treatment." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0171.

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Albumin is in contact with all cells in a body. This major protein in a plasma accesses all tissues and organs and has a number of different roles. Albumin was found to have more than 50 posttranslational modifications (PTMs). Some of the albumin PTMs showed correlation with tumorigenesis. Examples of PTMs of albumin are reported at www.phosphosite.org. Modifications like glycation of patients with breast cancer is seen higher as compared to healthy control. We hypothesize that several novel post-translational modification in albumin could be related to cancer and can be used as biomarkers. We performed mass spectrometry and 2D gel electrophoresis analysis of serum albumin for 32 most common PTMs. We identified most of these PTMs in albumin. We observed that human cancer cells affected PTMs profile of albumin. Examples of affected PTMs are phosphorylation, palmitolylation, geranyl- geranylation etc. We observed also differences in PTMs profiles of albumin from serum of a healthy person and cancer patient. O - GlcNAcylation, farnesylation, glutathionylation, S- nitrosylation etc PTMs were found to differ. Our data show that PTMs of albumin can be easily detected. Our trial with 32 PTMs can be expanded to detect up to a hundred known PTMs. These PTMs may correlate with cancer development, and may be used as markers in cancer diagnostic and prognostic.
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Arumbuliyur Comandur, Kaushik, Ali Asgar S. Bhagat, Subhashish Dasgupta, Ian Papautsky, and Rupak K. Banerjee. "Electroosmotic Injection and Chemical Kinetics in Micro Reactors." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-193050.

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The study of fluid flow in microchannels is of significant interest due to its application in a wide area of fields ranging from microscale flow injection, cooling of microchips, fuel vaporizer and micro reactors for chemical and biological systems. Design of effective electrokinetic micro reactors requires in-depth understanding of the electrokinetic phenomena and bulk reactions of species in the micro reactor. Although electrokinetic flows are popularly used for applications in the field of capillary electrophoresis (CE) [1], the phenomena of electroosmosis can be conveniently used for bulk transport and mixing of reagents. Electroosmosis occurs when the electrical double layer (EDL) near a solid-liquid interface is created by an external electric field. The uniqueness of electroosmotic flow (EOF) is characterized by plug velocity profile having uniform flow. Devasenathipathy et al. [2] showed that EOF offers a number of significant advantages over conventional pressure driven flow like reduced sample diffusion and controlled sample movement.
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Karkar, Slim, Lauren E. Alfonse, Catherine M. Grgicak, and Desmond S. Lun. "Statistical Modeling of Short-Tandem Repeat Capillary Electrophoresis Profiles." In 2018 IEEE International Conference on Bioinformatics and Biomedicine (BIBM). IEEE, 2018. http://dx.doi.org/10.1109/bibm.2018.8621135.

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Shao, Zhanjie, Gerry E. Schneider, and Carolyn L. Ren. "Theoretical Investigation of On-Chip Multi-Species Transport in Micro-Channels for Analysis Applications." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-41276.

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A complete mathematical model is developed for application to simulate the unsteady two-step on-chip sample injection and separation processes in microfluidic devices. The origin and applicability of the slip-wall velocity boundary condition is discussed. Due to electrophoresis effect, migration influence of every species is considered in the model and then solved for separation analysis. The model is non-dimensionalized in a unique manner to reveal effects of some key fundamental parameters: the Reynolds-Schmidt number, electrophoretic mobility of sample species, applied potentials, etc. In particular, the influence of ReSci is examined over the commonly encountered range and the effect of electrophoretic mobilities on separation is investigated for three different types of samples. Results include center-line concentration profiles as well as concentration contour plots over a range of nondimensional time (less than 400). Resolution is defined and employed to evaluate the separation results. The magnitude of calculated separation resolution (around 2.0) is comparable to experimental results. Through parametric studies, the characteristics of both injection and separation are revealed numerically and well understood for future effective control and innovative chip design.
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Farache Trajano, Luiza, Rebecca Moore, and Quentin Sattentau. "The Presence of Chemical Cross-Linking Stabilises HIV-1 Envelope Glycoprotein Trimer Antigens in a Model of Intramuscular Immunisation." In Building Bridges in Medical Science 2021. Cambridge Medicine Journal, 2021. http://dx.doi.org/10.7244/cmj.2021.03.001.4.

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Background: The HIV-1 envelope glycoprotein (Env) is the target of antigen design for antibody- based vaccination. In 2019, four trimeric Env vaccines entered an experimental trial: ConM, ConS, and their cross-linked counterparts. The trimers were formulated with MPLA adjuvant. Studies have demonstrated that adjuvants trigger neutrophil infiltration. Neutrophils activate and degranulate releasing proteases, namely elastase and cathepsinG. Aims: To assess the stability and immunogenicity of these vaccines in the presence of adjuvant- recruited neutrophils and their proteolytic enzymes. Methods: Trimers were incubated with commercially-sourced proteases. To analyse stability, samples were reduced, denatured and separated using gel electrophoresis. To assess antibody binding, a trimer-protease incubation was followed by an ELISA. To establish more physiologically relevant conditions, harvested neutrophils were exposed to various adjuvants. The supernatant, shown to contain elastase, was incubated alongside the vaccines. The reducing and denaturing gels, as well as the ELISA, was repeated. Results: Gel analysis revealed that un-crosslinked trimers underwent significant digestion whereas cross-linking conferred enhanced stability. In the presence of neutrophil-sourced protease-containing-supernatant, trimers displayed resistance to digestion. The differential stability profile of Env trimers when exposed to commercially sourced compared to supernatant- derived proteases may be due to the inhibitory effect of human serum on elastase. Antibody epitopes were maintained in vitro. Conclusion: The vaccine antigens are sensitive to enzymatic degradation. This is reduced by cross-linking and human serum.
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