Dissertations / Theses: 'Electrophoretic analysis'

1

Xue, Xin. "Electrohydrodynamically dried whey protein, electrophoretic and calorimetric analysis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape11/PQDD_0007/MQ44316.pdf.

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Xue, Xin 1972. "Electrohydrodynamically-dried whey protein : electrophoretic and calorimetric analysis." Thesis, McGill University, 1997. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=20605.

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Drying is an energy intensive process. The conventional heat-based drying methods often produce changes in the physico-chemical properties of products. A newly developed electrohydrodynamic (EHD) drying technique may be much less destructive to these heat-sensitive materials. This thesis presents comparative analyses of product deterioration in EHD-dried whey proteins, using electrophoresis, differential scanning calorimetry (DSC), and color measurements. Gel electrophoresis showed the disappearance of bands and reduction in band intensities depending upon the temperature of the oven in which the whey protein was dried. The thermograms of the differential scanning calorimeter varied considerably as the temperature of oven-drier increased. EHD, air-drying, and their combination showed no significant change in the electrophoretograms and thermograms compared with the native protein. Color measurements also indicated no significant change in color of EHD-dried whey protein whereas oven-drying produced darker colors from the original. These results allowed us to conclude that physico-chemical properties of whey protein remained intact after drying with EHD.

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GonzaÌlez, TunÌƒoÌn Maria Pilar. "Electrophoretic field gradient focusing for the analysis of proteins." Thesis, University of Leeds, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439600.

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Mo, Xiaoran. "Modelling and analysis of 2-D electrophoretic gel images." Thesis, University of Warwick, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.400103.

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SALAZAR, ZAZUETA ALFREDO JAVIER. "SOLUBILITY AND ELECTROPHORETIC PROPERTIES OF PROCESSED SAFFLOWER SEED (CARTHAMUS TINCTORIUS) PROTEINS." Diss., The University of Arizona, 1986. http://hdl.handle.net/10150/183972.

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Whole safflower seeds of the Mexican variety Kino'76 with a protein content of 17.30% (dwb) were subjected to the processes of dehulling, defatting (n-hexane extraction) and debittering (70% methanol extraction) to produce four types of meals preparations: whole safflower meal, dehulled safflower meal, debittered, whole meal and debittered, dehulled meal with protein contents of 26.90, 66.93, 26.70 and 69.92%, respectively. The proteins of each meal were studied in detail by means of protein fractionation, gel filtration chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Osborne solubility fractionation of the protein of whole safflower meal showed that the amount of protein in the alkali soluble fraction was approximately 71% of the total and the alcohol soluble fraction did not contain any protein. After dehulling and debittering, the amount of protein in the alkali soluble fraction decreased by 30%, whereas the amount of protein in the insoluble residue increased by 12%. SDS-PAGE of the proteins of the water-, salt- and alkali soluble fractions revealed that they consisted of 8, 13 and 13 distinct subunits, respectively, with apparent molecular weights ranging from 14.7 to 88.0 kDa. The number of subunits and molecular weight distribution decreased as a result of debittering. Fractionation of the proteins of each meal by gel filtration chromatography followed by SDS-PAGE demonstrated that proteins of safflower seed are highly heterogeneous. The process of debittering caused major alteration of the molecular weight profile and subunit composition of the gel filtration protein fractions.

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McLean, William Henry Irwin. "Electrophoretic and immunological analysis of proteins in the muscular dystrophies." Thesis, Queen's University Belfast, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335992.

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Hooks, Philip N. "Electrophoretic analysis of a single locus of Thamnochortus insignis Mast." Thesis, University of Cape Town, 2017. http://hdl.handle.net/11427/26127.

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8

Okamoto, Yukihiro. "Fundamental studies on electrophoretic analysis using functionalized particles and capillaries." 京都大学 (Kyoto University), 2007. http://hdl.handle.net/2433/136194.

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9

Severs, J. C. "Capillary electrophoretic and mass spectrometric analysis of some pharmaceutical and agrochemical compounds." Thesis, Swansea University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.638796.

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The relatively new separation technique of capillary electrophoresis, with its ability to provide rapid, high resolution separations based on electrophoretic mobility differences, has developed enormously over the last decade. Although the technique was first interfaced to mass spectrometry within a few years of its conception, when the studies described here were undertaken there were few reports of the use of such combined systems. The objectives of these studies were to conduct further investigations into the interfacing of capillary electrophoresis with mass spectrometry via atmospheric-pressure ionisation sources. The system was then to be used for the analysis of some polar and ionic compounds of interest to the pharmaceutical and agrochemical industries. The fundamentals of capillary electrophoresis, mass spectrometry and combined systems are reviewed. Interfaces developed in these studies for capillary electrophoresis-mass spectrometry for a range of capillary electrophoresis instruments and atmospheric-pressure ionisation sources are described and detailed practical instructions for their use are given. The results obtained for the separation and detection of analytes in three sets of compounds; some herbicide analogues, some β-blocker drugs and some small peptides are presented in Chapter 3. Transient capillary isotachophoresis methods have been developed and optimised for all three sets of analytes, improving concentration detection limits by at least an order of magnitude. The use of small-diameter capillaries has also been shown to improve sensitivity. Separation of two structurally similar herbicide analogues has been effected by the addition of an optimised concentration of a cyclodextrin to the electrophoresis buffer, and use of cyclodextrins in capillary electrophoresis-electrospray ionisation systems has been studied. Use of deuterated buffers in the combined system has been shown to improve certain separations and, through mass spectra showing deuterium exchange, provide further structural information for analysis. Practical considerations for successful completion of such studies are described.

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Sze, Kwan-Lok, and 施均樂. "Electrophoretic methodologies for the determinations of minerals and trace elements in milk." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43085246.

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Reilly, John. "A comparison of electrophoretic and chromatographic separation techniques for the determination of chiral and achiral impurities." Thesis, Imperial College London, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270880.

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Gupta, Ruchi. "Analysis of cellulose degrading enzymes in genetically modified sugarcane juice using capillary electrophoretic techniques." Thesis, University of Manchester, 2011. https://www.research.manchester.ac.uk/portal/en/theses/analysis-of-cellulose-degrading-enzymes-in-genetically-modified-sugarcane-juice-using-capillary-electrophoretic-techniques(6d34991e-bbbb-47e8-ab2e-e7293d5d9b17).html.

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Genetically modified (GM) sugarcane expressing cellobiohydrolase (CBH), endo-β-glucosidase (EG) and β glucosidase (BG) have a potential to provide low cost enzymes required for commercially viable production of ethanol from waste such as bagasse. The expression of enzymes in GM sugarcane is likely to be dependent on the variety of the plant and its growing conditions. In this project, reliable, rapid and easy-to-use methods capable of on-site analysis of cellulases in every batch of sugarcane juice (arriving hourly) were developed. Firstly, a capillary zone electrophoresis (CZE) method was devised for the separation and quantification of BG, CBH and EG, which were purified from sugarcane juice via ultrafiltration, in a single run. Under optimised conditions (15 mM phosphate buffer at pH 7.75), the analysis was achieved in 10 min. The linear dynamic range and the limit of detection of the developed method for the quantification of enzymes were suitable for monitoring their expected concentration in GM sugarcane juice. A novel isotachophoresis (ITP) method was then formulated for the purification of cellulases from sugarcane juice. An electrolyte system comprising a leading electrolyte of 10 mM formic acid at pH 9.0 and a terminating electrolyte of 10 mM β-Alanine was devised. The use of a simple front cutting method removed a majority of interfering species in the juice, thereby resulting in the formation of a distinct zone of desired proteins. In comparison to techniques such as ultrafiltration and liming, the loss of desired proteins was lower when the sample was prepared using ITP. A procedure for collecting fractions prepared via ITP was also formulated. The time required for sample clean-up of proteins in 10, 5 and 2 times diluted juice via the developed ITP method is ~30 min, ~45 min and ~60 min respectively. In other words, the enzymes in two-times diluted sample can be purified in ~60 min following which their concentration can be measured in ~10 min. This implies that the developed methods can be used to analyse sugarcane batches which are expected to arrive approximately every hour. The type of sugarcane and the agronomic conditions are also likely to affect the glycosylation of cellulases. The glycosylation plays a significant role in determining the enzyme activity. Hence, for the first time, CZE assays for the analysis of the glycoforms of CBH in an intact form were developed in this work. The developed methods were capable of analysing the glycoforms without the necessity of buffer additives or enzyme labelling protocols. The separation of the glycoforms of CBH was achieved using 15 mM, pH 4.5 and 50 mM, pH 7.5 phosphate electrolytes in a fused silica and a neutral capillary respectively. As the migration times of the glycoforms of CBH were highly repeatable in the latter method, its use is recommended for routine testing of glycosylation-mediated microheterogeneities in CBH. Finally, it is believed that the sample preparation and the analysis methods described in this thesis could serve as the basic operating principles of a microfluidic chip (called an Integrated Cellulase Unit, ICU). It is also envisioned that a cartridge containing multiple ICUs could be located at the end of the direct insertion probe to analyse cellulases at various points in the bagasse-to-bio-ethanol process.

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McGrath, Gareth. "The determination of selected drugs and endogenous molecules by modern electrophoretic, chromatographic and voltammetric techniques." Thesis, University of Ulster, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241755.

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Maruyama, Kazunori Ph D. Mie University. "Genome sequencing technology : improvement of the electrophoretic sequencing process and analysis of the sequencing tool industry." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/34868.

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Thesis (M.B.A.)--Massachusetts Institute of Technology, Sloan School of Management; and, (S.M.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering; in conjunction with the Leaders for Manufacturing Program at MIT, 2005.
Includes bibliographical references.
A primary bottleneck in DNA-sequencing operations is the capacity of the detection process. Although today's capillary electrophoresis DNA sequencers are faster, more sensitive, and more reliable than their precursors, high purchasing and running costs still make them a limiting factor in most laboratories like those of the Broad Institute. It is important to run those sequencers as efficiently as possible to reduce costs while producing robust assemblies. Polymer media for electrophoresis is the most important determinant for sequencing throughput. This thesis investigates the effect of polymer media on the performance of Applied Biosystems (ABI) 3730xl, the de-facto standard of DNA sequencers and develops analysis procedures for ABI3730xl system and its data. Due to its use in the human genome project (HGP), ABI has established a monopolistic position in the DNA-sequencing tool industry. As the de-facto standard of DNA sequencers ABI3730xl is highly automated, well-optimized, and black-boxed, despite the importance of higher throughput sequencing for diagnostic applications, third parties have found it difficult to improve sequencing methods.
(cont.) This thesis also conducts an analysis of the DNA-sequencing tool industry to discuss how ABI has established current monopolistic status, what kind of business model would be attractive for ABI in the post-HGP period, how new companies can successfully enter this industry, and how they can keep improving DNA-sequencing throughput along the line of "Moore's law".
by Kazunori Maruyama.
S.M.
M.B.A.

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15

Peterson, Zlatuše D. "Analysis of clinically important compounds using electrophoretic separation techniques coupled to time-of-flight mass spectrometry /." Diss., CLICK HERE for online access, 2004. http://contentdm.lib.byu.edu/ETD/image/etd410.pdf.

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Peterson, Zlatuse Durda. "Analysis of Clinically Important Compounds Using Electrophoretic Separation Techniques Coupled to Time-of-Flight Mass Spectrometry." BYU ScholarsArchive, 2004. https://scholarsarchive.byu.edu/etd/23.

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Capillary electrophoretic (CE) separations were successfully coupled to time-of-flight mass spectrometric (TOFMS) detection for the analysis of three families of biological compounds that act as mediators and/or indicators of disease, namely, catecholamines (dopamine, epinephrine, norepinephrine) and their O-methoxylated metabolites (3-methoxytyramine, norepinephrine, and normetanephrine), indolamines (serotonin, tryptophan, and 5-hydroxytryptophan), and angiotensin peptides. While electrophoretic separation techniques provided high separation efficiency, mass spectrometric detection afforded specificity unsurpassed by other types of detectors. Both catecholamines and indolamines are present in body fluids at concentrations that make it possible for them to be determined by capillary zone electrophoresis coupled to TOFMS without employing any preconcentration scheme beyond sample work up by solid phase extraction (SPE). Using this hyphenated approach, submicromolar levels of catecholamines and metanephrines in normal human urine and indolamines in human plasma were detected after the removal of the analytes from their biological matrices and after preconcentration by SPE on mixed mode cation-exchange sorbents. The CE-TOFMS and SPE methods were individualized for each group of compounds. While catecholamines and metanephrines in urine samples were quantitated using 3,4-dihydroxybenzylamine as an internal standard, deuterated isotopes, considered ideal internal standards, were used for the quantitation of indolamines. Because the angiotensin peptides are present in biological fluids at much lower concentrations than the previous two families of analytes, their analysis required the application of additional preconcentration techniques. In this work, the coupling of either of two types of electrophoretic preconcentration methods - field amplified injection (FAI) and isotachophoresis (ITP) - to capillary zone electrophoresis with both UV and MS detection was evaluated. Using FAI-CE-UV, angiotensins were detected at ~1 nM concentrations. Using similar conditions but TOFMS detection, the detection limits were below 10 nM. ITP was evaluated in both single-column and two-column comprehensive arrangements. The detection limits achieved for the ITP-based techniques were approximately one order of magnitude higher than for the FAI-based preconcentration. While the potential usefulness of these techniques was demonstrated using angiotensins standards, substantial additional research would be required to allow these approaches to be applied to plasma as part of clinical assays.

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17

Miller, John Francis. "The determination of very small electrophoretic mobilities of dispersions in non-polar media using phase analysis light scattering." Thesis, University of Bristol, 1990. http://hdl.handle.net/1983/45ef1314-ee97-4667-b179-a073b73d568d.

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An apparatus is described that can determine electrophoretic mobilities of polar and non-polar colloidal dispersions down to -12 2 10 msV -1 -1 with typical resolutions of 0.5 to 5%, depending on the nature of the dispersion being studied. The diffusion coefficient and settling/convection velocity of the sample may be determined simultaneously in real time with the electrophoretic mobility. The technique, phase analysis light scattering (PALSY, is based upon classical laser-Doppler electrophoresis, but employs signal processing of the time domain phase information within the scattered light signal, rather than analysis of its frequency spectrum. This allows much smaller electric field strengths to be employed, thereby alleviating the usual heating problems associated with electrophoretic studies of non-polar dispersions. PALS measurements of typical aqueous latex dispersions with large mobilities and non-polar dispersions with very small mobilities (down to 5x 10_12 m2 s-1 V-1) are presented to illustrate the versatility of the technique.

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18

Lysý, Jakub. "Posouzení vhodnosti termální vody pro relaxačně terapeutické využití." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-216633.

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My thesis is based on an assessment of the suitability of thermal spring in the Southern Moravia for a relaxing, therapeutic purposes. By capillary electrophoresis are measured concentration of natural substancies, which are used in thermal waters and are searched for therapeutic effects.

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Penn, Sharron Gaynor. "Chiral analysis by capillary electrophoresis." Thesis, University of York, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241074.

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20

Sohn, Dosung. "Invertebrates analysis by capillary electrophoresis." [Gainesville, Fla.] : University of Florida, 2009. http://purl.fcla.edu/fcla/etd/UFE0024340.

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21

Zhang, Zheru. "Single cell analysis by capillary electrophoresis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0012/NQ59703.pdf.

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Mangin, Catherine M. "Analysis of carrageenans using capillary electrophoresis." Thesis, University of York, 2000. http://etheses.whiterose.ac.uk/14043/.

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This thesis reports the use of capillary electrophoresis (CE) for the analysis of carrageenans, anionic polysaccharides extracted from red seaweeds and widely used in the food industry for their gelling and thickening properties. The three main types, kappa, iota and lambda, differ in the number of sulfate groups and the presence or absence of a 3,6-anhydro bridge in the disaccharide residue repeat unit. CE separates analytes according to their charge to frictional coefficient ratios, therefore it is suitable to separate these biopolymers. In order to detect polysaccharides in CE, our approach consisted in derivatising the reducing ends of the saccharides by reductive arnination with a fluorophore, l-arninopyrene-3,6,8- trisulfonate (APTS). This allowed sensitive detection by laser induced fluorescence. Method development gave optimal conditions for separation using a polyvinyl alcohol coated capillary and a 25 mM ammonium acetate, pH 8.0 background electrolyte. The effects of changes of both instrumental parameters (temperature, injection mode, field strength) and, the composition of the BGE (concentration and pH) are reported, and explained in terms of the physical chemistry of the BGE and the biopolymers. The conditions of the derivatisation reaction were studied in order to minimise degradation due in particular to acid catalysis and to reduction of the reacting sites occurring in competition with derivatisation. Characterisation of the derivatised carrageenans by SEC-MALLS- RI was performed and showed that the extent of degradation occurring during the labelling reaction was a maximum of 40 % for kappa and 20 % for iota and lambda. The presence of the label APTS in excess and its reaction with the reagents during the labelling reaction produces peaks interfering with those from the carrageenan. A sample clean-up was therefore required before injection onto CEo A comparison was made of a range of clean-up procedures (centrifugation, dialysis, preparative SEC) to remove side products of the reaction and salts and to concentrate the carrageenans. Various seaweed extracts were analysed, including standards of carrageenans not available commercially. This study revealed that carrageenans are complex structures, and often occurring as hybrids between sUb-types. CE has the ability to characterise these hybrids, unlike spectroscopic methods which detect individual residues. When using actual food products, preliminary steps such as defatting and dialysis were found to be necessary to allow satisfactory detection of carrageenans. Finally the strategy for sample purification, derivatisation, clean-up and separation was successfully applied to additive mixtures used as raw materials in the food industry and to finished products (jelly, dairy products). CE has proved to be a fast and sensitive method to identify and provide semi-quantitative information on carrageenans present in such mixtures.

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23

Örnskov, Eivor. "Physicochemical and Biopharmaceutical Characterisation of Small Drug Molecules by Capillary Electrophoresis." Doctoral thesis, Uppsala University, Analytical Chemistry, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4008.

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Capillary Electrophoresis (CE) was explored as a means for physicochemical and biopharmaceutical characterisation of small drug molecules. Special attention was paid to the characterisation of acid-base and lipophilic properties of drug compounds by analysing their migration behaviour in different CE systems. The thesis comprises an overview of the field together with separate studies on the different topics.

The utility of CE for the determination of pK_a of labile drug compounds was investigated. A general methodology was developed comprising key steps such as the use of a stabilising sample diluent, electromigration injection, and analyte characterisation by UV-Vis spectroscopy. The methodology was successfully applied for two sets of drug compounds, labile at low and high pH, respectively.

CE was also evaluated for experimental modelling of passive intestinal membrane permeability by studying analyte migration in liposomal, microemulsion and micellar electrolytes. Good correlation is reported between CE migration and Caco-2 cell absorption estimates and for in vitro inhibition of thrombin. Interestingly, a slightly better correlation was obtained for liposomal electrolytes.

The utility of liposomes in CE was further extended by developing a novel procedure for immobilising liposomes inside fused silica capillaries. This approach enabled direct on-line coupling of liposome CE to high sensitivity mass spectrometry. The utility of liposome-coated capillaries is demonstrated for estimating drug passive intestinal membrane permeability. Its use in biopharmaceutical drug profiling is discussed.

Utilising advanced molecular descriptors, commonly applied to in silico prediction of passive intestinal membrane permeability, migration of analytes in micellar CE systems could be well predicted. The novel approach was based on hierarchical multivariate analytics and use of molecular descriptors for both analytes and micellar media surfactants. Demonstrated results propose that the CE format could be useful to validate how representative molecular descriptors are for describing molecular behaviour in complex liquid media, e.g. physiological systems.

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Thyboll, Pettersson Erik. "Charged colloids observed by electrophoretic and diffusion NMR." Doctoral thesis, KTH, Chemistry, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-119.

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The thesis deals partly with methodology including construction

of hardware and new pulse sequences in the field of electrophoretic

NMR, and partly with practical use of ENMR and

diffusion NMR in the investigation of charged colloidal systems.

Several sources of artefacts are investigated, including gas production

at the electrodes, electroosmosis and Joule heating

effects that can cause convection. The electrophoretic double

stimulated-echo pulse sequence is introduced to suppress these

artefacts and to increase the feasible measuring range to higher

electric fields and conductivities.

The interaction between the non-ionic polymer poly(ethylene

oxide) PEO and differently charged surfactants is investigated

using the above mentioned methods. The investigated surfactants

are the anionic sodium dodecyl sulphate (SDS) and

potassium laurate (KC12), the cationic dodecyltrimethylammonium

bromide (CTAB) and the non-ionic octyl β-D-glucoside.

ENMR is also used to investigate two different mixed micelle

systems, with SDS as the charged surfactant component and

dodecyl malono-bis-N-methylglucamide (C12BNMG) respectively

tetra(ethylene oxide) dodecyl ether (C12EO4) as the nonionic

surfactant component. A method to calculate the degree

of counter-ion dissociation, αdissociation, as a function of composition

is demonstrated.

Finally diffusion NMR is used to compare transport dynamics

in gel electrolyte systems based on two differently grafted polymers;

one amphiphilic system containing polymethacrylate

grafted partly with polyethylene oxide and partly with fluorocarbons

and the corresponding nonamphiphilic system grafted

with only polyethylene oxide. Both systems contain the electrolyte

lithium bis(trifluoromethylsulfonyl) imide salt dissolved in

γ-butyrolactone. The results show that the system based on the

amphiphilic polymer has better transport dynamics and therefore

is more suited as material for battery

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McLeod, George Slater. "Development of capillary electrophoresis and capillary electrophoresis-mass spectroscopy methods for application in food analysis." Thesis, University of East Anglia, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267331.

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Smith, Justin R. Ewing Andrew Graham. "Analysis of biogenic amine neurotransmitters with capillary electrophoresis." [University Park, Pa.] : Pennsylvania State University, 2009. http://etda.libraries.psu.edu/theses/approved/WorldWideIndex/ETD-4415/index.html.

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Nhujak, Thumnoon. "Quantitative aspects of capillary electrophoresis and chiral analysis." Thesis, University of York, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270036.

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Randall, Greg C. "Single molecule analysis of DNA electrophoresis in microdevices." Thesis, Massachusetts Institute of Technology, 2006. http://hdl.handle.net/1721.1/34162.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2006.
Includes bibliographical references (p. 159-167).
Given that current electrophoresis technology is inadequate for mapping large O[100 kilobasepair] DNA, several promising lab-on-chip designs for DNA mapping have been recently proposed that require either 1) a DNA molecule negotiating an obstacle course in a microchannel or 2) stretching a DNA molecule for linear analysis. The goal of this research is to experimentally probe the fundamental physics that underlie these DNA mapping designs. Furthermore, we present a continuous stretching process that offers significant improvement over results in literature. In general, the governing physics is complex due to the confinement of the microchannel, the coiled-nature of long DNA molecules, and the induced electric field gradients from obstacles and changes in channel dimensions. Using single DNA fluorescence microscopy, we have investigated many of the governing physical mechanisms at play in these gene mapping microfluidic devices. For example, we have determined transport coefficients for DNA in a confined channel and performed an analysis of hydrodynamic background flows in these thin channels. We have also performed a systematic study of the impact and unhooking dynamics of a DNA molecule driven into a small stationary post.
(cont.) Furthermore, we have thoroughly investigated DNA stretching in electric field gradients created by a contraction and a large insulating obstacle. Just as a flow gradient stretches a polymer, an electric field gradient can stretch a charged polyiner like DNA. Because electric field gradients have no local rotational components, a charged polymer will experience purely extensional deformation. This is true even near surfaces, unlike the cyclic extension-compression dynamics characteristic in shear flows. A recurring theme is this work is the notion of configuration effects, or "molecular individualism", a term coined by DeGennes eight years ago to denote the sensitive dependence of a single polymer's deformation dynamics to its initial configuration. New mapping designs often require or force DNA to strongly deform and we see molecular individualism arise when studying phenomena central to these mapping designs, e.g. DNA colliding into stationary posts or DNA stretching in electric field gradients. Molecular individualism counteracts mapping and other potential engineering applications that might require uniform behavior of all DNA molecules. However, our results show that molecular individualism can be bypassed by pre-configuring the DNA configurational distribution before strong deformation.
(cont.) The main impact of these findings will be to foster the design of custoil unit operations in biomolecule microfluidic processes, e.g. for DNA mapping applications and beyond.
by Greg C. Randall.
Ph.D.

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29

Currie, Christa Anne. "Capillary and Microchip Electrophoresis Systems for Pharmaceutical Analysis." University of Cincinnati / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1242998601.

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Lalloo, Anita Kantilal. "Application of capillary electrophoresis for the assay of erythromycin and its related substance." Thesis, Rhodes University, 1997. http://hdl.handle.net/10962/d1003243.

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Capillary Electrophoresis (CE) is a high resolution analytical technique that may be employed in the separation and quantification of a wide range of analytes. The enormous efficiency obtained in CE are well suited for complex mixtures in which resolution of a large number of peaks in a short time is desirable. Therefore, CE has a promising future in pharmaC-eutical analysis. The separation mechanism of CE is based on the differential electrophoretic mobility of the solutes inside a buffer filled capillary upon the application of a voltage. Capillary electrophoresis is especially suitable for ionic species. The full potential of this technique can only be realised through the manipulation of numerous experimental parameters. In the present study, a CE method has been developed for the analysis of the macrolide antibiotics: erythromycin, oleandomycin, troleandomycin and josamycin. The selection of initial analysis conditions and optimisation of selectivity are reviewed. A systematic approach to method development was used to maximise analyte differential electrophoretic mobilities, by adjusting the pH. Thereafter, the influences of electrolyte molarity and electrolyte additives were investigated. In addition, some instrumental parameters, such as capillary length emf diameter, applied voltage and injection conditions were varied. The effect of the sample solvent and oncapillary concentration techniques such as FASI, were investigated. Also, the influence of injecting a water plug on the quantity of sample injected was demonstrated. Full resolution was achieved with the addition of methanol to the electrolyte. The applicability of CE for the assay of erythromycin and its related substances was investigated. Two methods were developed and successfully validated using CE: one for the quantitative determination of erythromycin alone and another for erythromycin related substances in the presence of large quantities of erythromycin A. Several related substances and impurities that result from the fermentation process used to produce erythromycin as well as degradation products are known to be present in commercial sa~ples. These impurities include erythromycin B, C, D, E, F, erythromycin enol ether, anhydroerythromycin and N-demethylerythromycin. Currently both the USP and BP official assays for the analysis of erythromycin involve the use of microbiological assays. These methods are limited as they are unable to differentiate between erythromycin and its related substances and degradation products. Furthermore, the microbiological assays are time-consuming and tedious to perform. 11 The CE methods developed for the analysis of erythromycin and for its related substances were fully validated in terms of precision, linearity, accuracy, sensitivity and stability. In addition, erythromycin was subjected to six stress modes and the stressed samples were analysed. An intemal standard was employed to provide acceptable precision for the migration time « 1.80 % RSD) and peak area « 4.44 % RSD). Optimum sensitivity was obtained using low UV wavelengths, with LOO values of less than 10 % for the related substances. The developed method was accurate for erythromycin C, anhydroerythromycin and N-demethylerythromycin, even in the presence of large concentrations of the parent. The method for~ erythromycin related substances was applied to the determination of impurities in three commercial erythromycin bases. The CE methods developed were rapid, precise, specific and stability-indicating and may be used to provide additional information to augment that attained by HPLC for purity assessment and in stability studies of erythromycin. Capillary electrophoresis is a simple, cost-effective technique that is capable of generating high quality data. This technique will become firmly established within pharmaceutical analysis for main peak and related impurity determination assays as familiarity becomes more widespread across the pharmaceutical industry and improvements in instrumentation are performed.

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31

Wong, Vicky. "Analysis of Arabidopsis xylans using polysaccharide analysis of carbohydrates using gel electrophoresis." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613790.

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32

Zhang, Yingbo. "Microfluidic methods for biomolecular analysis." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/274931.

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Microfluidics is the science and technology of manipulating fluids at small scales ranging from microlitres (10$^{-6}$) to picolitres (10$^{-12}$). The fundamental physics is distinct from fluid behaviour on bulk scales and laminar flow is the key characteristic on this scale. Microfluidic systems have a wide range of applications in many disciplines from engineering to physics, chemistry and biotechnology. In this thesis, I explore different strategies exploiting the capabilities of microfluidic devices for manipulating and analysing biomolecules. A particular focus of the work is on the study of amyloid fibrils. These species are protein aggregates related to a wide range of human diseases and functional materials. In chapter 3 I demonstrate an efficient way to separate particles in different sizes based on a microfluidic diffusion method. This method enables us to explore the properties of amyloid fibrils, such as their growth kinetics and interaction with small molecules. Rapid binding information could be obtained with only microlitres of sample in tens of seconds time scale. A further manipulation method for charged particles is introduced in chapter 4, based on the integration of microfluidics and free flow electrophoresis. I present a very effective and simple way to overcome one of the most critical problem in this situation. High electric filed can be applied through two streams of conductive solutions, with all the electrolysis by products, e.g., gas bubbles and other deposits, removed simultaneously without interfering with the system. In addition to microfluidic devices made by soft lithography in PDMS, I also set up a hot embossing fabrication process with the Teflon material (chapter 5). Teflon has many advantages compared with PDMS, such as lower protein adsorption, higher mechanical strength and better chemical compatibility. With different materials and structures, microfluidic devices can be expanded to more applications.

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33

Voss, Karl O. "Capillary electrophoresis for DNA sequencing and cytosine methylation analysis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ29120.pdf.

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Pinto, Devanand Michael. "Trace analysis of peptides and proteins by capillary electrophoresis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ34822.pdf.

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35

Olmo, Stefano <1978&gt. "Capillary coatings in Capillary Electrophoresis (CE) analysis of Biomolecules." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/1071/.

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36

Goyder, Miriam Sarah. "Capillary electrophoresis with multiple readout techniques for protein analysis." Thesis, Imperial College London, 2011. http://hdl.handle.net/10044/1/9004.

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In the era of proteomics, new technologies in separation and identification are required. Separation methods, such as capillary electrophoresis or liquid chromatography, are a crucial part of high throughput proteomic workflows. In this thesis, novel approaches to proteomics using capillary electrophoresis are presented. A platform of technologies based on capillary electrophoresis with continuous deposition of separated proteins onto metallic substrates enables subsequent analyses and identification. Since sample deposition and identification are decoupled, multiple readout techniques can be explored. Readout techniques used include matrix assisted laser desorption/ionisation mass spectrometry (MALDI-MS), electron-vibration-vibration two dimensional infrared spectroscopy (EVV 2DIR) and fluorescence microscopy. This technology was used without the deposition interface, to achieve advances in ribosomal separations or with the deposition interface, to develop new proteomic strategies of separation and readout. The eukaryotic ribosomal proteins were separated using capillary electrophoresis for the first time. Over 26 peaks were resolved in less than 10 minutes. An outstanding RSD migration time of < 0.5% was achieved, demonstrating that the readout could provide a ribosomal ' fingerprint'. Separations of proteins were successfully analysed using a standard MALDIMS instrument. This work was advanced by the offline coupling of CE to MALDI-imaging and applied to the ribosomal proteins to demonstrate a novel workflow from cell culture to protein identification. Quantitative analysis of protein levels is an important part of proteomics, but is difficult to achieve using mainstream technologies with high throughput and accuracy. EVV 2DIR is a non-linear spectroscopy which is able to achieve absolute quantification of proteins.[1] Coupling of EVV 2DIR to CE (CE- 2DIR) was demonstrated through the deposition and analysis of peptide and proteins. CE-2DIR offers great promise as a new proteomic tool.

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37

Yang, Weichun. "Integrated Affinity Column Capillary Electrophoresis Microdevices for Biomarker Analysis." BYU ScholarsArchive, 2010. https://scholarsarchive.byu.edu/etd/2341.

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In this dissertation, microfluidic systems that integrate antibody-based sample preparation methods with electrophoretic separation are developed to analyze multiple biomarkers in a point-of-care setting. To form an affinity column, both monolith materials and wall-coated channels were explored. I successfully demonstrated that monolith columns can be prepared in microfluidic devices via photopolymerization. The selectivity of monolith columns was improved by immobilizing antibodies on the surface. These affinity columns can selectively enrich target analytes and reduce the signal of contaminant proteins up to 25,000 fold after immunoaffinity extraction. These results clearly demonstrate that microchip affinity monoliths can selectively concentrate and purify target analytes through specific antibody-antigen interactions. These monolith columns operated well for simple systems such as buffered solution, but suffered from clogging with real biological samples such as human serum. Therefore, I developed new affinity columns using a wall coating protocol. To form the affinity columns, a thin film of a reactive polymer was UV polymerized in a microchannel. Antibodies were attached by reaction between the polymer epoxy groups and antibody amine groups. All steps, including loading, washing, and elution for affinity extraction, as well as capillary electrophoresis analysis, were achieved simply via applying voltages to reservoirs on the microdevice. By adding reservoirs containing alpha-fetoprotein (AFP) standard into the same device, a quantitative method, either standard addition or calibration curve, can also be performed on-chip. These polymer microdevices have been applied in determining AFP levels in spiked serum samples, and the results are comparable with the values measured using a commercial enzyme linked immunosorbent assay kit. These microchips have also been adapted for detection of multiple biomarkers by immobilizing different antibodies on the affinity column. Four kinds of antibodies were attached to microchip columns, and the amounts of immobilized antibodies were characterized. The fluorescence signals of all four protein antigens were in the same range after rinsing, indicating that the derivatization reaction had little bias toward any of the four antibodies. With spiked human blood serum samples, four proteins in the ng/mL range were simultaneously quantified using both calibration curves and standard addition. In general, the calibration curve and standard addition results were close to the known spiked concentrations. These results indicate that my integrated microdevices can selectively retain and analyze targeted compounds in clinical samples. Moreover, my platform is generalizable and applicable for the simultaneous quantification of multiple biomarkers in complex matrices.

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38

Garcia, Agnes D. "The use of capillary electrophoresis (CE) in drug analysis." FIU Digital Commons, 2005. https://digitalcommons.fiu.edu/etd/3928.

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Capillary electrophoresis is currently a very powerful technique for the analysis of seized drugs. A rapid analytical CE method for the screening and quantification of GHB and GBL was achieved using 300mM CTAC/25mM phosphate buffer pH 6.3. Reversed phase HPLC was achieved using 25mM phosphate buffer pH 6.5 and a Cl8 Aqua column. Chiral separation of 9 amphetamine type stimulants was obtained using a highly sulfated gamma-cyclodextrin as a chiral selector. MECC and CZE were compared for the analysis of psilocybin, while a rapid and robust method is presented for the analysis of major opium alkaloids, using dynamically coated capillary columns. The column is coated with a polycation followed by a polyanion coating, using a commercial reagent kit. Using a background electrolyte pH of 2.5 with the addition of hydroxypropyl-beta- cyclodextrin and dimethyl-beta-cyclodextrin, the analysis of morphine, papaverine, codeine, noscapine, and thebaine in opium samples was obtained with great resolution. Finally, separation of common benzodiazedpines was also investigated using CZE and a pH 2.5 phosphate buffer.

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RIBEIRO, JUNIOR MARCOS A. "Estudo comparativo do veneno de Bothrops jararaca do continente e de especimes da Ilha de Sao Sebastiao." reponame:Repositório Institucional do IPEN, 2008. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11746.

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Made available in DSpace on 2014-10-09T12:55:22Z (GMT). No. of bitstreams: 0
Made available in DSpace on 2014-10-09T14:05:55Z (GMT). No. of bitstreams: 0
Dissertação (Mestrado)
IPEN/D
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

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40

Dahlén, Johan. "Capillary electrophoresis and multivariate calibration in the analysis of natural waters /." Linköping : Tema, Univ, 1999. http://www.bibl.liu.se/liupubl/disp/disp99/arts199s.htm.

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41

Klockow-Beck, Antje. "Analysis of carbohydrates in food and glycoconjugates with capillary electrophoresis /." [S.l.] : [s.n.], 1995. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=11241.

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42

Rye, Morten Beck. "Image segmentation and multivariate analysis in two-dimensional gel electrophoresis." Doctoral thesis, Norwegian University of Science and Technology, Department of Chemistry, 2007. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-1744.

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The topic of this thesis is data-analysis on images from two-dimensional electrophoretic gels. Because of the complexity of these images, there are numerous steps and approaches to such an analysis, and no “golden standard” has yet been established on how to produce the desired output. In this thesis focus is put on two essential fields concerning 2D-gel analysis; registration of images by segregation and protein spot identification, and data-analysis on the output of such a registration by multivariate methods. Image segmentation is mainly concerned with the task of identifying individual protein spots in a gel-image. This has generally been the natural starting point of all methods and procedures developed since the introduction of 2D-gels in the mid-seventies, simply because this best reproduces the results created by a human analyst, who manually identify protein-spot entities. The amount of data produced in a 2D-gel experiment can be quite large, especially in multiple gels where the human analyst is dependent on additional statistical data-analytical tools to produce results. Because of the correlated nature of most gel-data, analysis by multivariate methods is natural choice, and are therefore adopted in this thesis. The goal of this thesis is to introduce the above mentioned procedures at different stages in the analysis pipeline where they are not yet fully exploited, rather than to improve already existing algorithms. In this way new insight and ideas on how to handle data from 2D-gel experiments are achieved. The thesis starts with a review of segmentation methodology, and introduces a selected procedure used to identify protein spots throughout. Output from the segmentation is then used to create a multivariate spot-filtering model, which aims to separate protein spots from noise and artefacts often creating problems in 2D-gel analysis. Lately the use of common spot boundaries in multiple gels have been the method of choice when gels are analysed. How such boundaries should be defined is an important subject of discussion, and thus a new method for defining common boundaries based on the individual segmentation of each gel is introduced. Segmentation may be a natural starting point when gels are analysed, but it is not necessarily the most correct. Often the introduction of fixed spot entities introduces restrictions to the data which cause problems at later stages in the analysis. Analysing pixels from multiple gels directly has no such restrictions, and it is shown in this thesis that the output of such an analysis based on multivariate methods can produce very useful results. It can also give insight to the data problematic to achieve with the spot boundary approach. At last in the thesis an improved pixel-based approach is introduced, where a less restricted segmentation is used to reduce and concentrate the amount of data analysed, improving the final output.

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43

Burn, Ross Thomas. "Human plasma proteome analysis using chromatography, electrophoresis and mass spectrometry." Thesis, Queen Mary, University of London, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500312.

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The human plasma proteome is potentially rich in novel biomarkers for the diagnosis, prognosis of disease and drug development. In the quest for low abundant biomarkers, the problems associated with the complexity of sample and vast dynamic range in concentration have to be overcome. Such strategies include the depletion of the most abundant proteins from plasma, the employment of prefractionation techniques and multidimensional separations. Mass spectrometry can be used to identify and quantify these biomarkers. The most abundant proteins can be depleted from plasma using antibody or dye based affinity columns. Several abundant protein depletion kits were investigated with the aim of selecting the most effective kit for subsequent analyses.

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44

Thakur, Anup P. "METABOLITE ANALYSIS OF CLOSTRIDIUM THERMOCELLUM USING CAPILLARY ELECTROPHORESIS BASED TECHNIQUES." UKnowledge, 2008. http://uknowledge.uky.edu/gradschool_diss/647.

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Clostridium thermocellum is a thermophilic bacterium that converts biomass to ethanol directly; however, high sensitivity of this bacterium toward ethanol limits its commercial utility. To elucidate the effect of ethanol on the growth of this bacterium a metabolite analysis of C. thermocellum was performed. The hypothesis of the project was that exogenous ethanol alters the metabolite profile of C. thermocellum. For metabolite analysis, capillary electrophoresis-electrospray ionization-mass spectrometry method (CE-ESI-MS) was developed due to highly polar and charged nature of metabolites. To increase the sensitivity of CE-ESI-MS, several parameters at the ESI interface were optimized. The application of 50% isopropanol as a sheath liquid increased sensitivity for metabolite analysis dramatically. Trimethylamine acetate (pH 10) was used as background electrolyte (BGE) due to its ability to separate the structural isomers of glucose phosphate. For metabolite sample preparation, novel methods for quenching and CE compatible metabolite extraction protocols were developed. Newly developed protocols were applied to metabolite analysis of wild type (WT) and ethanol adapted (EA) strains of C. thermocellum grown in batch cultures. Significant differences were found in key intracellular metabolites such as NAD+ and pyruvic acid. Intracellular concentrations of NAD+ were low in EA cells compared to WT cells and pyruvic acid was only detected in EA cells. To further understand the effect of ethanol on metabolite fluxes, WT and EA cells were grown in increasing concentrations of ethanol and the metabolite profile for each ethanol treatment was obtained. Significant changes were found in intracellular metabolite concentrations. Metabolic data showed that the glycolysis process in WT cells was obstructed due to exogenous ethanol which was evident from accumulation of G6P. On the other hand, no such accumulation of G6P was observed in the EA strain; however pyruvate began to accumulate in EA strain. These changes in intracellular metabolite concentrations due to perturbation of exogenous ethanol supported the hypothesis. Also, this investigation revealed a correlation between ethanol and metabolite profile changes and was able to explain a possible mechanism of growth inhibition of C. thermocellum which will certainly help genetic engineers to develop superior strains of C. thermocellum for commercial cellulosic ethanol production.

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45

Owen, Peter David. "The application of capillary electrophoresis to the analysis of isocyanates." Thesis, Open University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287073.

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46

Smith, John Eric. "Capillary electrophoresis in the analysis and characterisation of lubricant additives." Thesis, Sheffield Hallam University, 2004. http://shura.shu.ac.uk/20666/.

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Capillary electrophoresis (CE) has proved to be a very efficient separation method in aqueous media. This work reports developments in nonaqueous capillary electrophoresis (NACE) for samples from the lubricants industry. Two commonly used additives in lubricants, zinc dialkyldithiophosphate and the metallic detergent alkylsalicylate, were analysed. Method development included selection of solvent, pH, and electrolyte type and concentration. Improvements in the separation were obtained by altering the injection conditions and the electric field strength. Hexadimethrine bromide (HDB) was shown to be essential in controlling the electroosmotic flow (EOF), and its concentration was optimised. Best separation was seen when analyte dilution prior to injection was carried out with methanol, resulting in sample stacking. Analysis of ZDDPs showed that separation occurred through migration of free dithiophosphate ions. Calcium alkylsalicylate showed similar results where the free alkylsalicylate ions migrated. Identification was achieved through peak patterns and relative migration times. Adequate separation conditions were identified and then coupling of NACE to mass spectrometry was attempted. Direct infusion MS was first utilised with great success in identification of the two additives. Using a solution of methanol and ammonium acetate it was possible to identify free dithiophosphate ions from the ZDDP and free alkylsalicylate ions from magnesium alkylsalicylates. Only the molecular ions were obtained in all instances making identification easier. NACE-MS was carried out successfully; however, sample dilution and adequate interfacing were problematic. Analysis of formulated lubricating oil was performed by NACE, NACE-MS and MS. Degradation products were identified for ZDDPs, where sulphur atoms were replaced with oxygen leading to the formation of phosphates. NACE has been shown to be a technique of potential for the analysis of lubricant additives, and in combination with mass spectrometry it could be very powerful.

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Lundqvist, Anders. "Nanoscale biochemical analysis using capillary electrophoresis and confocal fluorescence detection /." Göteborg : Göteborg university, 2002. http://catalogue.bnf.fr/ark:/12148/cb40115032p.

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48

Keski-Hynnilä, Helena. "Liquid chromatography - and capillary electrophoresis - mass spectrometry in glucuronide analysis." Helsinki : University of Helsinki, 2002. http://ethesis.helsinki.fi/julkaisut/mat/farma/vk/keski-hynnila/.

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49

Chiang, Han-Wei, and 江翰威. "Surface analysis of electrophoretic deposition graphene oxide using Taguchi method." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/8vwf49.

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碩士
中原大學
機械工程研究所
106
In this study, Taguchi Methods were used to synthesize powdered graphene oxide by Hummers method. The graphene oxide was deposited on indium tin oxide (ITO) substrate by electrophoretic deposition, and the surface morphology was observed and found electrophoretic deposition of the best conditions. In Taguchi''s L9(34) orthogonal arrays parameters, graphene oxide was electrophoretically deposited by changing parameters such as voltage and anode distance, temperature and weight percent concentration. Graphene oxide deposited heart-shaped pleated appearance differences can be observed by scanning electron microscope , and also observed the phenomenon of graphite stacking characteristics; Energy dispersion spectrometer instrument results show that carbon and oxygen elements in graphene oxide are majority, and the ratio close to 1: 1, means that the oxidation effect is good. In addition, the experimental results show that in the contact angle measurement, the maximum control factor of the S/N ratio reaction factor is the temperature. Secondary is concentration .Under the conditions of the parameters of the voltage of 7V, cathode anode distance 14 mm, temperature 55℃and concentration 1.5% , a minimum angle of 28 degrees was obtained. Atomic force microscope measurement results showed that the root mean square roughness Rq was 30.1nm and the center line average roughness Ra was 19.6nm under conditions of voltage of 7V, adistance of 14mm of cathode and anode, temperature of 55℃and concentration of 1.5% . The roughness is relatively smooth. S/N ratio of the maximum reaction factor control factor and contact angle measurement results are the same temperature is the maximum control factor. In this experiment, through the contact angle measurement and atomic force microscope results, the temperature can be obtained as the maximum control factor through the S/N ratio calculation, and the optimal parameters were found to be 5V, cathode anode distance 18mm, temperature 40℃, concentration 1% . By the surface structure and nature of the experimental analysis, to enhance the future of postgraduate medical technology, corrosion resistance, electrode efficiency, polymer composite materials, paint use.

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Lin, Guei-Jung, and 林桂忠. "Research on Analysis of Nano TiO2 Photocatalyst by Electrophoretic Deposition Method." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/m3d98f.

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碩士
國立臺北科技大學
能源與冷凍空調工程系碩士班
98
The study intends to understand the effectiveness of degradation of ammonia gas by photocatalysis of titanium oxide (TiO2). First of all, TEM and XRD are used to observe the particle size and structure of TiO2 nanopowder, Degussa P25 respectively. After that, TiO2 nanopowder film is deposited on a stainless steel substrate by electrophoretic deposition (EPD). Different electric field intensities, solvents, suspension concentrations and temperatures are the parameters of electrophoretic experiment. Film thickness gauge is used to observe the deposition situation of TiO2 thin film under different parameters. After that, SEM and EDS are used to observe the surface distribution of the deposited thin film and the situation of its ingredients after high-temperature sintering. In the photocatalytic experiment, ultra violet / visible light spectrophotometer is used as an instrument to inspect ammonia gas all through the experiment. The study explores the effects of vaporization of ammonia water of different concentrations, different heat treatment temperatures of thin films and different deposition thicknesses on photocatalysis of ammonia gas. Experiment result shows that the deposited TiO2 thin film can be controlled by EPD parameters. However, it is found that with the thickness increase of the deposited TiO2 thin film, the cracks on the surface enlarges accordingly, thus being disadvantageous to EPD for a long time. Besides, as shown from the result of photocatalytic experiment, when the temperature of heat treatment is 400oC, the photocatalytic effect of TiO2 thin film can reach 17%. With the thickness increase of the deposited thin film, the contact of ammonia gas with the surface area of thin film is increased, and its photocatalytic effect can reach 24.2%. Finally, it can be found that when the concentration of TiO2 thin film is further reduced, the photocatalytic effect is the best, achieving 26.7%. By means of EPD, the study is able to rapidly control the preparation of deposited TiO2 thin film, and effectively degrade ammonia gas. In future, EPD can be applied to purify air and improve the environmental quality.

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Dissertations / Theses on the topic 'Electrophoretic analysis'

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