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Journal articles on the topic 'ELISA (Enzyme Link Immunosorbent Assay)'

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Published: 3 June 2025

Last updated: 31 July 2025

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1

Bassett, Margaret E., David J. Thornton, John K. Sheehan, and Ian A. Nieduszynski. "An enzyme-linked immunosorbent assay (ELISA) of denatured cartilage link protein." Biochimica et Biophysica Acta (BBA) - General Subjects 925, no. 3 (1987): 347–55. http://dx.doi.org/10.1016/0304-4165(87)90201-7.

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Azimi, Nooshin T., Fujiang Wen, Richard M. Lister, Dennis A. Chen, and Fred E. Lytle. "Enzyme-Linked Immunoassays Using Nanosecond Fluorometric Detection." Applied Spectroscopy 46, no. 6 (1992): 994–98. http://dx.doi.org/10.1366/0003702924124402.

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Nanosecond temporal resolution is combined with an enzyme-linked immunosorbent assay (ELISA) to improve the lower limit of detection for a plant virus, brome mosaic virus. The method uses alkaline phosphatase as the enzyme link and β-naphthyl phosphate as the substrate. Enzymatic activity produces the highly fluorescent tag β-naphthol. The 8.9-ns lifetime of the tag allows temporal discrimination against the assay blank, providing a 64× improvement in the detection limit as compared to a steady-state measurement, and a ∼4100× improvement over a standard ELISA method incorporating the chromogen

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Jaishree, Jatwa. "HYPOTHYROIDISM OCCURS DUE TO IODINE DEFICIENCY, THE HORMONAL ASSAY T3, T4 AND TSH DONE BY ELISA TEST IN UJJAIN DISTRICT MP INDIA." International Journal of Research - GRANTHAALAYAH 3, no. 9 (Special Edition) (2017): 1–3. https://doi.org/10.5281/zenodo.852173.

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Hypothyroidism is a ‘Public health problem occurs due to Iodine deficiency and it is mostly described in Human population; significantly it is more common in female, than males. Interpretation of the TSH level depends upon the level of thyroid hormone; T3, T4 and TSH, low value of T3, T4 and high value of TSH defines thyroid disorders; Hypothyroidism and its viceversa condition is Hyperthyroidism, the hormonal assay done by ELISA (Enzyme Link Immunosorbent Assay) test.

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Sacks, J. M., K. G. Gillette, and G. H. Frank. "Development and evaluation of an enzyme-linked immunosorbent assay for bovine antibody to Pasteurella haemolytica: Constructing an enzyme-linked immunosorbent assay titer." American Journal of Veterinary Research 49, no. 1 (1988): 38–41. https://doi.org/10.2460/ajvr.1988.49.01.38.

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SUMMARY A 2-stage strategy was developed and evaluated for estimating serum antibody titer by use of elisa and a series of dilutions. In stage 1, the linear response region and least-square estimate of the assay line slope were established from 9-point dilution assays. Provided that the reading was within the linear response region, this information was used in the stage-2 estimation of titer from a single absorbance reading. Operationally, 2 fixed dilutions were selected, one suitably low and one suitably high, to provide at least one reading within the linear region. The procedure should sav

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JaishreeJatwa. "HYPOTHYROIDISM OCCURS DUE TO IODINE DEFICIENCY, THE HORMONAL ASSAY T3, T4 AND TSH DONE BY ELISA TEST IN UJJAIN DISTRICT MP INDIA." International Journal of Research -GRANTHAALAYAH 3, no. 9SE (2015): 1–3. http://dx.doi.org/10.29121/granthaalayah.v3.i9se.2015.3123.

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Hypothyroidism is a ‘Public health problem occurs due to Iodine deficiency and it is mostly described in Human population; significantly it is more common in female, than males. Interpretation of the TSH level depends upon the level of thyroid hormone; T3, T4 and TSH, low value of T3, T4 and high value of TSH defines thyroid disorders; Hypothyroidism and its vice-versa condition is Hyperthyroidism, the hormonal assay done by ELISA (Enzyme Link Immunosorbent Assay) test.

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Hardjoutomo, S., and M. B. Poerwadikarta. "Anthrax retrospective analysis in endemic areas using enzyme-linked immunosorbent assay (ELISA)." Jurnal Ilmu Ternak dan Veteriner 2, no. 2 (2014): 127–31. https://doi.org/10.14334/jitv.v2i2.58.

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About the end of 1995, an infectious disease among buffaloes as well as human beings was reported to occur in the District of Ngada, East Nusatenggara . Specimens of death animals, namely a piece of bones, hide, soil samples as well as blood and exudate smears and sera of the local inhabitants were submitted to Balitvet, Bogor laboratory for diagnosis . Bacteriological examinations of those specimens revealed that B. Anthracis was the causative agent of the disease . Along the line, 40 humans sera comprised 20 sera of local exposed inhabitants and 20 sera of non-exposed outsider, examined by t

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7

Lima, Monique da Rocha Queiroz, Rita Maria Ribeiro Nogueira, Ana Maria Bispo de Filippis, and Flavia Barreto dos Santos. "Comparison of Two Generations of the Panbio Dengue NS1 Capture Enzyme-Linked Immunosorbent Assay." Clinical and Vaccine Immunology 18, no. 6 (2011): 1031–33. http://dx.doi.org/10.1128/cvi.00024-11.

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ABSTRACTWe compared two generations of Panbio (Brisbane, Australia) commercial kits for NS1 antigen capture for early diagnosis of dengue: the first-generation pan-E Dengue Early ELISA and the second-generation Dengue Early ELISA. The test improvement resulted in a highly sensitive and specific test suitable for use as a first-line test in the field.

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8

R N, Mohammed, Alegbejo M D, Banwo O O, and Kashina B D. "Department of Crop Protection, Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State, Nigeria." Nigerian Journal of Plant Protection 39, no. 1 (2025): 45–53. https://doi.org/10.71464/sr11r605.

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Field surveys were conducted in June 2016 wet and November 2017 dry seasons to determine the incidence of alternative hosts of sweet potato viruses in (6) Local Government Areas of Kaduna State namely; Giwa, Kudan, Soba, Igabi, Kachia and Zango-Kataf. One hundred and eight weed samples within and around the farms were collected in both seasons; Fourteen weeds were identified in wet season while 9 weeds were identified in dry season. Three viruses were tested; Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV) and Sweet potato virus (SPV2). The weeds were ide

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9

B., R. Alkali, I. Aisha B., F. Useh M., et al. "Enzyme link Immunosorbent Assay-based Detection of Hepatitis B Virus Infection in Correctional Facilities in Sokoto Metropolis, Sokoto State Nigeria." Journal of Advances in Medical and Pharmaceutical Sciences 13, no. 3 (2017): 1–9. https://doi.org/10.9734/JAMPS/2017/32199.

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ELISA-Based detection of HBV infection in correctional facilities in Sokoto metropolis, Sokoto state Nigeria was carried out using a questionnaire survey to determine the associated risk factors of the disease and serological method using [Enzyme link immunosorbent Assay (ELISA)] to determine hepatitis B surface antigen (HB<sub>S</sub>Ag) among inmates. Of the 180 participants from the two correctional facilities in Sokoto state (Sokoto central prison and Remand home) overall prevalence rate of (42.2%) was obtained. The distribution of HBV infection according to age was statistically significa

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10

Winger, Caroline M., Elizabeth U. Canning, and J. D. Culverhouse. "A monoclonal antibody toBabesia divergenswhich inhibits merozoite invasion." Parasitology 94, no. 1 (1987): 17–27. http://dx.doi.org/10.1017/s0031182000053415.

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SUMMARYA monoclonal antibody-producing hybridoma cell line has been raised against merozoites ofBabesia divergens. This antibody is strongly reactive against merozoites in an enzyme-linked immunosorbent assay (ELISA) and is also positive in an indirect fluorescent antibody test (IFAT) on preparations of live merozoites. The antibody recognizes an antigen, of molecular weight (Mr) 50–60 K by Western blot analysis on SDS–polyacrylamide gel electrophoresis (SDS–PAGE) gels. Merozoite neutralization assays show that the antibody significantly inhibits merozoite invasion of bovine erythrocytes.

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11

Mariani, Massimo, Enrico Luzzi, Daniela Proietti, et al. "A Competitive Enzyme-Linked Immunosorbent Assay for Measuring the Levels of Serum Antibody to Haemophilus influenzae Type b." Clinical Diagnostic Laboratory Immunology 5, no. 5 (1998): 667–74. http://dx.doi.org/10.1128/cdli.5.5.667-674.1998.

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ABSTRACT A competitive ELISA method is described for the measurement of total antibodies to the capsular polysaccharide ofHaemophilus influenzae type b (HibCPS) in human sera. The competitive method showed an excellent correlation to the radioantigen binding assay (RABA, or Farr assay) and improved correlation of sera with low titers with respect to the more conventional noncompetitive method. Overestimation of samples in the low concentration range was no longer observed with the competitive ELISA method. The free HibCPS competition allowed us to eliminate the day-to-day background variation

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12

HAZA, ANA I., PALOMA MORALES, ROSARIO MARTÍIN, et al. "Use of a Monoclonal Antibody and Two Enzyme-Linked Immunosorbent Assay Formats for Detection and Quantification of the Substitution of Caprine Milk for Ovine Milk." Journal of Food Protection 60, no. 8 (1997): 973–77. http://dx.doi.org/10.4315/0362-028x-60.8.973.

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A stable hybridoma cell line (B2B) has been produced that secretes a monoclonal antibody (MAb) specific for goat's milk αS2-casein. The MAb B2B was used in two enzyme-linked immunosorbent assay (ELISA) formats for the detection and quantification of the presence of goat's milk in ewe's milk. In the indirect ELISA format the limit of detection was 0.5 to 15% (vol/vol) substitution of goat's milk for ewe's milk. Afterwards, a competitive indirect ELISA was successfully developed for the detection of 0.25 to 15% (vol/vol) of goat's milk in ewe's milk. This competitive indirect ELISA is a very sen

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13

Galula, Jedhan U., Gwong-Jen J. Chang, Shih-Te Chuang, and Day-Yu Chao. "Establishment of an Algorithm Using prM/E- and NS1-Specific IgM Antibody-Capture Enzyme-Linked Immunosorbent Assays in Diagnosis of Japanese Encephalitis Virus and West Nile Virus Infections in Humans." Journal of Clinical Microbiology 54, no. 2 (2015): 412–22. http://dx.doi.org/10.1128/jcm.02469-15.

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The front-line assay for the presumptive serodiagnosis of acute Japanese encephalitis virus (JEV) and West Nile virus (WNV) infections is the premembrane/envelope (prM/E)-specific IgM antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA). Due to antibody cross-reactivity, MAC-ELISA-positive samples may be confirmed with a time-consuming plaque reduction neutralization test (PRNT). In the present study, we applied a previously developed anti-nonstructural protein 1 (NS1)-specific MAC-ELISA (NS1-MAC-ELISA) on archived acute-phase serum specimens from patients with confirmed JEV and WNV

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14

Park, Kuen-Woo, and Min-Jea Kim. "CMV-RESISTANT CULTIVAR SELECTION BY ENZYME-LIKED IMMUNOSORBENT ASSAY AND PROTOPLAST ISOLATION METHOD IN CUCUMBER." HortScience 27, no. 6 (1992): 672a—672. http://dx.doi.org/10.21273/hortsci.27.6.672a.

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This experiment was carried out to select resistant cultivar to CMV in cucumber using Elisa-test and protoplast isolation method. Twenty domestic cultivars or lines and 8 European cultivars were ested for resistance by Elisa test. Among the domestic cultivars, DADAKI group was found to be susceptible and CHEONGJANG group resistant. Among all the cultivars and lines tested, a European cultivar, DALIBOR and Janghyungheukjinju Korean line were found to be highly resistant. When comparing for the protoplast yield depending upon the positions of seedlings (cotyledon, young leaf and hypocotyl), the

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Jordan, Robert A., Mark T. Preissler, Jeffrey A. Banas, and Edmund J. Gosselin. "Production of Genetically Engineered Biotinylated Interleukin-2 and Its Application in a Rapid Nonradioactive Assay for T-Cell Activation." Clinical Diagnostic Laboratory Immunology 10, no. 3 (2003): 339–44. http://dx.doi.org/10.1128/cdli.10.3.339-344.2003.

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ABSTRACT The development of reliable assay systems that can measure lymphocyte activation in vitro has been a major goal of immunodiagnostics. Traditionally, tritiated thymidine incorporation has been used to monitor T-cell activation. Other methods include enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot assay, and colorimetric assays. We have established a lymphocyte activation assay that utilizes fluorescein isothiocyanate (FITC)-streptavidin bound to recombinant biotinylated human interleukin-2 (IL-2). Utilizing recombinant DNA technology, a unique monobiotinylated human

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16

Bugari, G., C. Poiesi, A. Beretta, S. Ghielmi, and A. Albertini. "Quantitative immunoenzymatic assay of human lutropin, with use of a bi-specific monoclonal antibody." Clinical Chemistry 36, no. 1 (1990): 47–52. http://dx.doi.org/10.1093/clinchem/36.1.47.

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Abstract In this immunoenzymatic assay for human lutropin (hLH) we used bi-specific antibodies (BiAbs) obtained from the fusion of two hybridomas producing antibodies to beta-D-galactosidase and hLh. The BiAb complexed with the enzyme beta-D-galactosidase was used as tracer in a double-determinant assay. We compared the assay involving the BiAb (Bi-EIA) with an immunoenzymatic assay (EIA) in which the same capture antibody was used but the tracer was an enzyme-conjugated hLH-specific monoclonal antibody produced by the same parental cell line used to produce the BiAb. The coefficient of correl

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17

Culver, J. N., J. L. Sherwood, and M. R. Sanborn. "Use of Monoclonal Antibodies in Detection and Serological Classification of Peanut Stripe Virus1." Peanut Science 16, no. 2 (1989): 63–66. http://dx.doi.org/10.3146/i0095-3679-16-2-1.

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Abstract Monoclonal antibodies (MABs) to peanut stripe virus (PStV) were obtained by fusing spleen cells from mice immunized with PStV to a mouse myeloma cell line. Two IgG2a subclass MABs, each binding with different antigenic sites, and rabbit polyclonal antibodies (PABs) were compared for their reaction to PStV and other serologically related plant viruses. One MAB reacted to PStV but not to other serologically related viruses. The other MAB reaction of the MABs and PABs to PStV in peanut leaf and seed tissues were compared by enzyme-linked immunosorbent assay (ELISA), dot-immunobinding ass

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18

Ochiai, H., S. Sakai, T. Kogure, T. Hirabaytashi, K. Nakajima, and K. Terasawa. "Development and some applications of enzyme-linked immunosorbent assay system for murine macrophage inflammatory protein-2 (MIP-2)." Mediators of Inflammation 5, no. 3 (1996): 206–9. http://dx.doi.org/10.1155/s0962935196000294.

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We attempted to establish an enzyme-linked immunosorbent assay (ELISA) system by preparation of recombinant murine MIP-2 and its rabbit antibodies. A fusion construct of MIP-2 to protein A was used to enable easy purification as well as the generation of a sufficiently large antibody response. The specificity of antibody was confirmed by Western blotting analysis of 20-h conditioned medium from lipopolysaccharide (LPS)-stimulated RAW264.7 cells, a murine macrophage cell line; antibody gave a single band with a molecular weight of approximately 6000, which is identical to that of murine MIP-2 r

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Yang, Xingdong, Yang Qu, Chenchen Wang, Lihua Wu, and Xiaofei Hu. "Development of an Enzyme-Linked Immunosorbent Assay Based on a Monoclonal Antibody for the Rapid Detection of Citrinin in Wine." Foods 14, no. 1 (2024): 27. https://doi.org/10.3390/foods14010027.

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The ingestion of food contaminated with citrinin (CIT) poses a variety of health risks to humans and animals. The immunogens (CIT-COOH-BSA, CIT-H-BSA) and detection antigen (CIT-COOH-OVA, CIT-H-OVA) were synthesised using the active ester method (-COOH) and formaldehyde addition method (-H). A hybridoma cell line (3G5) that secretes anti-CIT monoclonal antibodies (mAbs) was screened via CIT-H-BSA immunisation of mice, cell fusion, and ELISA screening technology. The cell line was injected intraperitoneally to prepare ascites. The reaction conditions for the indirect competitive ELISA (ic-ELISA

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KASUGA, Kuniko, Tomoko OGAWA, Masayoshi TOKIEDA, and Akio KITAYAMA. "Detection of Rubella Virus Antibody by Enzyme Linked Immunosorbent Assay with Parallel Line Method." Journal of the Japanese Association for Infectious Diseases 66, no. 4 (1992): 497–502. http://dx.doi.org/10.11150/kansenshogakuzasshi1970.66.497.

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Alam, Ahmed Nawsher, Mahmuda Siddiqua, Shahina Tabassum, and Md Nazrul Islam. "Comparison of Indirect Immunofluorescence Assay as a Confirmatory Test to Detect Antibody to Human Immunodeficiency Virus with Line Imuune Assay and Western Blot Methods." Bangladesh Journal of Medical Microbiology 2, no. 2 (2016): 12–16. http://dx.doi.org/10.3329/bjmm.v2i2.28840.

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In low prevalent countries of Human Immunodeficiency Virus (HIV) like Bangladesh, it is recommended that all HIVpositive sera tested by Enzyme Linked Immunosorbent Assay (ELISA) and/or rapid tests should be confirmed by Line Immune Assay (LIA) or Western Blot (WB) method. As these two tests are quite expensive, Indirect Immunofluorescence Assay (IFA) was evaluated as a confirmatory test and as an alternative to these two methods in the present study. A total of 92 subjects consisting of 46 HIV-antibody-positive patients and 46 controls were included in the study. All samples of sera were teste

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Debnath, Ashis, Dinesh C. Pathak, Narayan Ramamurthy, et al. "Serological profiling of rabies antibodies by enzyme-linked immunosorbent assay and its comparative analysis with rapid fluorescent focus inhibition test in mouse model." Veterinary World 12, no. 1 (2019): 126–30. http://dx.doi.org/10.14202/vetworld.2019.126-130.

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Aim: In this study, we have used enzyme-linked immunosorbent assay (ELISA) as an alternative test to replace the cumbersome rapid fluorescent focus inhibition test (RFFIT) to ascertain the immune status of immunized mice against rabies virus. Materials and Methods: Rabies is a devastating disease worldwide caused by rabies virus. Proper usage of pre- or post-exposure rabies vaccine can prevent the disease transmission. In this study, mice were immunized with Vero cell-adapted inactivated rabies vaccine. RFFIT was used as a test to determine the serum neutralizing titers in infected/vaccinated

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Paramita, Dewi K., Jajah Fachiroh, Sofia M. Haryana, and Jaap M. Middeldorp. "Two-Step Epstein-Barr Virus Immunoglobulin A Enzyme-Linked Immunosorbent Assay System for Serological Screening and Confirmation of Nasopharyngeal Carcinoma." Clinical and Vaccine Immunology 16, no. 5 (2009): 706–11. http://dx.doi.org/10.1128/cvi.00425-08.

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ABSTRACT Undifferentiated nasopharyngeal carcinoma (NPC; WHO type III) is 100% associated with Epstein-Barr virus (EBV) infection and the fourth most prevalent cancer in Indonesian males. Therapy failure is high, since most patients come to the hospital at an advanced stage of disease. Screening for early-stage NPC is needed. Here, a simple and economical two-step enzyme-linked immunosorbent assay (ELISA) system is proposed for diagnosing NPC in high-risk populations, employing the peptide-based immunoglobulin A (IgA) EBNA1 plus viral capsid antigen p18 ELISA as an initial screening test and t

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Lee, Brian W., Russell F. Bey, Mary J. Baarsch, Robert B. Morrison, and Wayne Freese. "Determination of hemagglutination-inhibition titers to influenza A virus in porcine sera by use of an enzyme-linked immunosorbent assay." American Journal of Veterinary Research 54, no. 8 (1993): 1270–76. http://dx.doi.org/10.2460/ajvr.1993.54.08.1270.

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Summary An elisa-based method to estimate hemagglutination-inhibition (hi) titer was developed. Subtype specificity was obtained by using purified H1 and H3 hemagglutinin antigens. Using the linear relation that exists between elisa and hi methods, regression lines for HIN1- and H3N2-monospecific porcine antisera were constructed. Approximation of actual hi titer could be obtained from insertion of elisa values into the appropriate regression line. The hi estimations were within 50% of the actual measured hi value 84% of the time. In young pigs that had suckled immune sows, use of this elisa r

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Wu, Te Hsing, Hong Ru Lin, Lie Hang Shen, and Jia Hau Liu. "Wound Healing Based on PU/Hydrogel Nanocompounds Containing FGF-2 and Related Dressings by Radiation Method." Advanced Materials Research 79-82 (August 2009): 437–40. http://dx.doi.org/10.4028/www.scientific.net/amr.79-82.437.

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PU/hydrogel compounds prepared byγ-ray radiation technique were investigated in this study. We used nanosized clay solution or powder, Polyurethane (PU), thermosensitive N-isopropyl acrylamide (NIPAAm) and acrylic acid (AAc) as starting materials to prepare PU/hydrogel nanocomposite products. The dipping and weighing tests, Material Testing Machine (LLOYD) were utilized to analyze the physical and mechanical properties differentially. In addition, the drug release and wound closure experiments were also finished by Enzyme-link immunosorbent assay (ELISA) reader and five groups of treatment dre

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Meguid, Nagwa A., Hazem M. Atta, Laila A. Rashed, Amr S. Gouda, Rehab O. Khalil, and Adel F. Hashish. "Autism and Fragile X: Is There a Neurochemical Link?" Open Access Macedonian Journal of Medical Sciences 2, no. 4 (2014): 551–56. http://dx.doi.org/10.3889/oamjms.2014.099.

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BACKGROUND:Autism and Fragile X syndrome are intertwined. This study aimed at assessing Serotonin, Glutamate, and Gama Amino Butyric Acid (GABA) in autism and Fragile X syndrome patients and to detect possible neurochemical similarities between the 2 disorders that can be used as metabolic biomarkers.DESIGN AND METHODS: Eighty subjects divided into four groups, two diseased groups (20 male patients with Autism and 20 males with Fragile X syndrome) and two control groups (20 neurotypical male controls and 20 Down syndrome male patients) were included. Estimation of Serotonin, Glutamate and GABA

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Van Manen-Brush, Kathleen, Jacob Zeitler, John R. White, Paul Younge, Samantha Willis, and Marisa Jones. "Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform." BioTechniques 69, no. 3 (2020): 186–92. http://dx.doi.org/10.2144/btn-2020-0074.

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Chinese hamster ovary (CHO) cells are a mammalian cell line used in the production of therapeutic proteins. Host cell proteins (HCPs) are process-related impurities that are derived from the host cell expression system. During biopharmaceutical drug development, removal of HCPs is required. Enzyme-linked immunosorbent assay (ELISA) is a common technique to quantitate HCPs, but is a labor-intensive process that takes up to 7 h. Ella® is an automated instrument that utilizes microfluidics and glass nanoreactors to quantitate HCPs in 75 min using similar ELISA reagents. The antibodies and antigen

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Poilane, Nadia, Madoka Takai, and Kazuhiko Ishihara. "Well Defined Surface Preparation with Phospholipid Polymers for Highly Sensitive Immunoassays." Key Engineering Materials 342-343 (July 2007): 889–92. http://dx.doi.org/10.4028/www.scientific.net/kem.342-343.889.

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Plastic substrates were modified with 2-methacryoyloxyethyl phosphorylcholine (MPC) copolymer brushes by surface-initiated atom transfer radical polymerization (ATRP) with a mean chain density of 2.44 chains/nm2 and a chain length around 50nm. Ellipsometry, X-ray photoelectron spectroscopy, gel permeation chromatography, nuclear magnetic resonance and atomic force microscopy were used to characterize the modified surfaces.MPC was copolymerized with a methacrylate monomer including an active ester unit which can link covalently with antibodies. They latter were immobilized on these well-defined

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Thomas, Prevenslik. "THE CAUSAL LINK BETWEEN VACCINATION ADJUVANTS AND AUTISM." International Journal of Research - Granthaalayah 7, no. 6 (2019): 288–95. https://doi.org/10.5281/zenodo.3332670.

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Adjuvants essential in vaccines to stimulate activation of the immune system comprise submicron ( 100 nm) nanoparticles (NPs) of aluminum hydroxide that upon vaccine injection cross the blood-brain-barrier (BBB) and because of their low solubility may remain in the brain for an extended time. Despite over a century of use, the mechanism underlying the stimulation of the immune system by adjuvants has not been understood. Solar UV can optically stimulate most immune systems in the skin, but solar UV is not available inside the brain. By the process of simple QED, the NPs lacking heat capacity

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Jones-Engel, Lisa, Katherine A. Steinkraus, Shannon M. Murray, et al. "Sensitive Assays for Simian Foamy Viruses Reveal a High Prevalence of Infection in Commensal, Free-Ranging Asian Monkeys." Journal of Virology 81, no. 14 (2007): 7330–37. http://dx.doi.org/10.1128/jvi.00343-07.

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ABSTRACT Foamy viruses (FV) are retroviruses that naturally infect many hosts, including most nonhuman primates (NHPs). Zoonotic infection by primate FV has been documented in people in Asia who reported contact with free-ranging macaques. FV transmission in Asia is a concern, given abundant human-NHP contact, particularly at monkey temples and in urban settings. We have developed three assays capable of detecting the presence of FV in Asian NHP species that are commensal with humans: enzyme-linked immunosorbent assay (ELISA), Western blot assays using recombinant viral Gag protein, and an ind

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Tamborrini, Marco, Marcelle Holzer, Peter H. Seeberger, Nadia Schürch, and Gerd Pluschke. "Anthrax Spore Detection by a Luminex Assay Based on Monoclonal Antibodies That Recognize Anthrose-Containing Oligosaccharides." Clinical and Vaccine Immunology 17, no. 9 (2010): 1446–51. http://dx.doi.org/10.1128/cvi.00205-10.

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ABSTRACT The similarity of endospore surface antigens between bacteria of the Bacillus cereus group complicates the development of selective antibody-based anthrax detection systems. The surface of B. anthracis endospores exposes a tetrasaccharide containing the monosaccharide anthrose. Anti-tetrasaccharide monoclonal antibodies (MAbs) and anti-anthrose-rhamnose disaccharide MAbs were produced and tested for their fine specificities in a direct spore enzyme-linked immunosorbent assay (ELISA) with inactivated spores of a broad spectrum of B. anthracis strains and related species of the Bacillus

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Dr., Enuneku Patrick Omuku, L.C. ORJI, A.U. ONUIGBO, and A.E OZUKWE. "APPLICATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR AFLATOXIN CONTENT EVALUATION OF DIFFERENT POWDERED MILK AVALABLE IN ANAMBRA STATE, NIGERIA." GPH-International Journal of Applied Science 06, no. 06 (2023): 01–10. https://doi.org/10.5281/zenodo.8119362.

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The application of enzyme-link immunosorbent assay for aflatoxin contamination of 20 different powdered milk samples consumed within Anambra State, Nigeria was carried out primarily to ascertain their health implications. The samples were subjected to Ridascreen ELISA competitive enzyme immunoassay for the quantitative determination of aflatoxins in the milk. All the reagents required for the enzyme immunoassay including statndards were contained in the test kit used. Preparatory samples stages were grinding, extraction, filtration/centrifugation followed by acid mixture digestion process with

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Sorscher, E. J., M. A. Accavitti, D. Keeton, E. Steadman, R. A. Frizzell, and D. J. Benos. "Antibodies against purified epithelial sodium channel protein from bovine renal papilla." American Journal of Physiology-Cell Physiology 255, no. 6 (1988): C835—C843. http://dx.doi.org/10.1152/ajpcell.1988.255.6.c835.

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Monoclonal and polyclonal antibodies against amiloride-sensitive sodium channel protein purified from bovine renal papilla have been produced. These antibodies show considerable specificity in enzyme-linked immunosorbent assay (ELISA) and dot-blot assays and can immunoprecipitate radiolabeled channel protein. The polyclonal antibodies bind two sodium channel subunits on Western blots, namely, the 300- and 110-kDa polypeptides, and cross react with channel protein isolated from the amphibian A6 renal epithelial cell line. They can be used to immunoaffinity purify the channel in relatively high

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Kommineni, K. V., J. M. Gillett, and D. C. Ramsdell. "A Study of Tomato Ringspot Virus and Prune Brown Line Resistance in Twenty-five Rootstock-Scion Combinations." HortTechnology 8, no. 3 (1998): 349–53. http://dx.doi.org/10.21273/horttech.8.3.349.

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In a greenhouse study, fifty 1-year-old `Stanley'/`Myrobalan 29C' plum (Prunus sp.) trees were inoculated with tomato ringspot nepovirus (ToRSV) by either nematode inoculation or slash inoculation to compare how inoculation effects the onset of the prune brown line (PBL) disease. In six tests (over 2 years), slash-inoculated trees had a higher percentage of ToRSV infection than nematode-inoculated trees when root and bark samples were tested by enzyme-linked immunosorbent assay (ELISA). ELISA differences between the two treatments were significant by chi-square analysis. None of the ToRSV posi

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Gunarti, Dwirini Retno, Dwirini Retno Gunarti, and Mardyana Nizar. "Application of Thiamine-Biotin Conjugate for Serum Thiamine Levels Measurement." Jurnal Biotek Medisiana Indonesia 10, no. 2 (2022): 155–62. http://dx.doi.org/10.22435/jbmi.v10i2.5830.

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ABSTRAK Tiamin berfungsi sebagai koenzim untuk beberapa enzim yang terlibat dalam metabolisme karbohidrat. Mengingat pentingnya peran tiamin, maka dilakukan pengembangan teknik pengukuran tiamin yang analog dengan enzyme linked immunosorbent assay (ELISA), dimana antibodi diganti dengan protein pengikat spesifik yaitu protein ikat tiamin kacang hijau (PITKH). Teknik pengukuran ini dilakukan secara kompetitif, kompetitor akan dikompetisikan dengan tiamin bebas yang akan diukur. Kompetitor tersebut berupa konjugat antara tiamin-biotin. Tiamin murni diikatkan dengan biotin menggunakan senya

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Perše, Gabrijela, Ivana Mareković, Sanja Pleško, Violeta Rezo Vranješ, and Marija Jandrlić. "Evaluation of Aspergillus Galactomannan Lateral Flow Assay on Serum and Bronchoalveolar Lavage Specimens." Infektološki glasnik 41, no. 2 (2022): 35–40. http://dx.doi.org/10.37797/ig.41.2.1.

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Background: Detection of biomarkers, such as galactomannan (GM), has proven to be of great significance in early recognition of invasive aspergillosis (IA). The aim of our study was to evaluate the lateral flow assay (LFA) for the detection of GM on serum and bronchoalveolar lavage (BAL) samples previously proven positive by enzyme-linked immunosorbent assay (ELISA). Methods: The study was performed on serum and BAL samples obtained from patients with suspected IA in the period from February 2019 to January 2020, which were previously GM positive by ELISA (Platelia Aspergillus Ag, Biorad, Herc

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NOAH, CHARLES W., SHERILYN S. POTEET, MILDRED M. LISTER, et al. "Production and Characterization of Monoclonal Antibodies To Detect Vibrio cholerae Serogroup O1 in a Rapid Enzyme-Linked Immunosorbent Assay." Journal of Food Protection 59, no. 11 (1996): 1153–57. http://dx.doi.org/10.4315/0362-028x-59.11.1153.

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Monoclonal antibodies (MAbs) were developed for a rapid and efficient screening procedure to detect cultures of Vibrio cholerae serogroup O1. Spleen cells of BALB/c mice previously immunized with an attenuated control strain of V. cholerae were fused with mouse myeloma cell line SP2/0. An enzyme-linked immunosorbent assay (ELISA) was used to test cultural hybridoma secretions of two MAbs against 120 strains of V. cholerae O1, 38 strains of V. cholerae non-O1, 15 strains of other Vibrio spp., and 20 strains of other bacterial species. Results of tests using both MAbs were identical. The MAbs su

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Prevenslik, Thomas. "THE CAUSAL LINK BETWEEN VACCINATION ADJUVANTS AND AUTISM." International Journal of Research -GRANTHAALAYAH 7, no. 6 (2019): 288–95. http://dx.doi.org/10.29121/granthaalayah.v7.i6.2019.805.

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Adjuvants essential in vaccines to stimulate activation of the immune system comprise submicron (< 100 nm) nanoparticles (NPs) of aluminum hydroxide that upon vaccine injection cross the blood-brain-barrier (BBB) and because of their low solubility may remain in the brain for an extended time. Despite over a century of use, the mechanism underlying the stimulation of the immune system by adjuvants has not been understood. Solar UV can optically stimulate most immune systems in the skin, but solar UV is not available inside the brain. By the process of simple QED, the NPs lacking heat capaci

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Zeyl, Victoria G., Haiying Xu, Imran Khan, et al. "CD30 Lateral Flow and Enzyme-Linked Immunosorbent Assays for Detection of BIA-ALCL: A Pilot Study." Cancers 15, no. 21 (2023): 5128. http://dx.doi.org/10.3390/cancers15215128.

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Introduction: Breast Implant-Associated Anaplastic Large Cell Lymphoma (BIA-ALCL) commonly presents as a peri-implant effusion (seroma). CD30 (TNFRSF8) is a consistent marker of tumor cells but also can be expressed by activated lymphocytes in benign seromas. Diagnosis of BIA-ALCL currently includes cytology and detection of CD30 by immunohistochemistry or flow cytometry, but these studies require specialized equipment and pathologists’ interpretation. We hypothesized that a CD30 lateral flow assay (LFA) could provide a less costly rapid test for soluble CD30 that eventually could be used by n

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Benhamou, Nicole, G. B. Ouellette, J. G. Lafontaine, and J. R. Joly. "Use of monoclonal antibodies to detect a phytotoxic glycopeptide produced by Ophiostoma ulmi, the Dutch elm disease pathogen." Canadian Journal of Botany 63, no. 7 (1985): 1177–84. http://dx.doi.org/10.1139/b85-163.

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Two hybridomas that secrete antibodies specific for a phytotoxic glycopeptide from Ophiostoma ulmi (Buism.) Nannf. were produced by fusing spleen cells of mice immunized with the purified toxin and the Sp2-0/Ag14 mouse myeloma cell line. Specificity of these antibodies was first demonstrated by an enzyme-linked immunosorbent assay (ELISA), then by immunoblotting on nitrocellulose membrane after sodium dodecyl sulfate – polyacrylamide gel electrophoresis of the glycopeptide. Both clones produced antibodies of IgM class as determined by immunodiffusion. These monoclonal antibodies were utilized

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Sokolov, A. V., V. A. Kostevich, N. V. Gorbunov, et al. "A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES." Medical Immunology (Russia) 20, no. 5 (2018): 699–710. http://dx.doi.org/10.15789/1563-0625-2018-5-699-710.

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Myeloperoxidase is a key factor promoting development of halogenative/oxidative stress under inflammatory conditions. Previously, we have discovered complexes including myeloperoxidase and its physiological inhibitor, ceruloplasmin in blood plasma of patients with inflammatory diseases of different etiology, e.g., atherosclerosis. Studies on regulation of myeloperoxidase activity by ceruloplasmin have shown that hypochlorous acid, a specific product of myeloperoxidase action, is likely to modify ceruloplasmin during inflammation. The present study was aimed for analysis of relationships betwee

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Guo, Jie Biao, Xuan Xin, Rui Min Zhong, and Xing Ping Li. "Development of a Biotin-Avidin Mediated Immunoassay for Simultaneous Detection of Danofloxacin, Enrofloxacin and Ciprofloxacin in Milk." Advanced Materials Research 781-784 (September 2013): 1630–36. http://dx.doi.org/10.4028/www.scientific.net/amr.781-784.1630.

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To generate a group-specific monoclonal antibody (McAb) against danofloxacin (DANO), enrofloxacin (ENR) and ciprofloxacin (CIP), glutaraldehyde was used to link the presentative hapten of CIP to the immunogen and coating antigen, respectively, leaving the common structure of these 3 drugs exposed in both conjugates as a major antigenic site. Consequently, a McAb (6D3) with high cross-reactivity to this three antibiotics has been obtained by using hybridomas technique. In a biotin-avidin mediated enzyme-linked immunosorbent assay, the IC50values for DANO, ENR and CIP were 5.1, 4.5 and 4.2 ng mL

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Salman, Omar D., Maysoon A. Z. Merdaw, and Ahmed A. Almaliky. "The Probable Association between Type 2 Diabetes Mellitus and Toxoplasma gondii Infection." INTERNATIONAL JOURNAL OF DRUG DELIVERY TECHNOLOGY 13, no. 01 (2023): 131–35. http://dx.doi.org/10.25258/ijddt.13.1.20.

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Background: Type 2 diabetes mellitus (T2DM) is a chronic disorder that constitutes a major health problem worldwide. Toxoplasma gondii is an intracellular parasite that may infect any nucleated cell. Toxoplasmosis is becoming a worldwide health threat, infecting 30–50% of the world’s human population. The studies that have been undertaken to investigate the link between T. gondii infection and diabetes have shown contradictory fi ndings. This research aimed to look at the possible link between T2DM and T. gondii infection. Methods and Subjects: The enzyme-linked immunosorbent assay (ELISA) app

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Mahieux, R., P. F. Lambert, E. Agbottah, et al. "Cell Cycle Regulation of Human Interleukin-8 Gene Expression by the Human Immunodeficiency Virus Type 1 Tat Protein." Journal of Virology 75, no. 4 (2001): 1736–43. http://dx.doi.org/10.1128/jvi.75.4.1736-1743.2001.

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ABSTRACT The human immunodeficiency virus type 1 (HIV-1) Tat protein has been reported to transactivate several cellular genes, including the potent chemotactic factor interleukin-8 (IL-8). Consistent with these in vitro assays, elevated levels of IL-8 protein are found in the serum of HIV-infected individuals. We now extend these observations by demonstrating that Tat induction of IL-8 is linked to the cell cycle. Cells that constitutively express the Tat(1–86) protein (eTat) and control cells (pCEP) were reversibly blocked at the G1/S border with hydroxyurea or thymidine. The cells were subs

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Chatlynne, Louise G., William Lapps, Michael Handy, et al. "Detection and Titration of Human Herpesvirus-8–Specific Antibodies in Sera From Blood Donors, Acquired Immunodeficiency Syndrome Patients, and Kaposi's Sarcoma Patients Using a Whole Virus Enzyme-Linked Immunosorbent Assay." Blood 92, no. 1 (1998): 53–58. http://dx.doi.org/10.1182/blood.v92.1.53.413k30_53_58.

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A human herpesvirus-8 (HHV-8) enzyme-linked immunosorbent assay (ELISA) with a whole virus lysate as antigen was developed and used to measure the seroprevalence rate and levels of IgG antibodies to HHV-8 in sera/plasma of various patient groups and blood donors. The virus antigen was prepared from the KS-1 cell line, which produces lytic virus, and therefore contains a broad array of viral proteins. Seroprevalence studies using this ELISA showed the following: 10 of 91 blood donors (11%) had an average HHV-8 antibody titer of 118; 67 of 72 (93%) classic Kaposi's sarcoma (KS) patients were pos

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Mwangi, David. "Indirect, Competitive Enzyme-Linked Immunosorbent Assay determination of Secretory Immunoglobulin A levels in saliva. (37.9)." Journal of Immunology 182, no. 1_Supplement (2009): 37.9. http://dx.doi.org/10.4049/jimmunol.182.supp.37.9.

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Abstract The amount of specific antibodies in the body can influence one's ability to fight or succumb to disease. Saliva provides the first line of defense against pathogens that enter the body through the mouth from the air or from ingested food. The primary antibody in saliva is salivary IgA. The students will complete a class activity that mimics the path taken by sIgA from its synthesis by plasma cells to its release from the mucosal membrane. In the first lab, students will construct a standard curve obtained from color intensity readings of serially diluted soft drink. This curve will b

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Firdaus, Muhamad, Fadhilah Purna Agustin, Alif J. Kholifatul, Nugroho Wiratama та Yusuf Adi Sudjatmiko. "ANTIOBESITY OF FUCOXANTHIN FROM Sargassumechinocarpum BY INCREASING β-OXIDATION IN ADIPOCYTE". KnE Life Sciences 2, № 1 (2015): 97. http://dx.doi.org/10.18502/kls.v1i0.91.

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Fucoxanthin is a one of carotenoids that contained in brown seaweeds. This compound has been affected to lipid metabolism. The aim of this study was to evaluate the antiobesity of fucoxanthin of Sargassumechinocarpum on adipocyte by increasing of adiponectin and decreasing of tumor necrosis factor a expressions. Fucoxanthin was isolated from Sargassumechinocarpum and validated by infrared spectrophotometer. Adipocyte was obtained from pre-adipocyte cell from viscera tissue of wistar rats (Rattusnorvegicus) and cultured in fetal bovine serum and Dulbeccos modified eagle’s medium. Adipocyte was

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Xu, Wenyan, and Linlin Zhao. "An Enzyme-Linked Immunosorbent Assay for the Detection of Mitochondrial DNA–Protein Cross-Links from Mammalian Cells." DNA 2, no. 4 (2022): 264–78. http://dx.doi.org/10.3390/dna2040019.

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DNA–Protein cross-links (DPCs) are cytotoxic DNA lesions with a protein covalently bound to the DNA. Although much has been learned about the formation, repair, and biological consequences of DPCs in the nucleus, little is known regarding mitochondrial DPCs. This is due in part to the lack of robust and specific methods to measure mitochondrial DPCs. Herein, we reported an enzyme-linked immunosorbent assay (ELISA)-based method for detecting mitochondrial DPCs formed between DNA and mitochondrial transcription factor A (TFAM) in cultured human cells. To optimize the purification and detection w

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Jaiswal, Anoop. "Myeloperoxidase and troponin T are linked with myocardial infarction among young Indians." Bioinformation 18, no. 1 (2022): 66–71. http://dx.doi.org/10.6026/97320630018066.

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It is of interest to document the correlation of myeloperoxidase (MPO) and Troponin T (TnT) in young Indian patients with myocardial infarction using epidemiological data. A study with 220 (110 case and 110 control) participants with Acute Coronary Syndrome (both males and females) was completed. Patients were identified using a pre-designed PROFORMA at the OPD and IPD of the Medicine Department at the Index Medical College & Hospital, Malwanchal University and Moti Lal Nehru Medical College and Swaroop Rani Nehru Hospital in India. MPO and TnT were measured using the Sandwich ELISA (Sandw

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Wu, Te Hsing, Hong Ru Lin, and Lie Han Shen. "Preparation and Properties of Nanocomposites Containing FGF-2, other Related Dressings and the Primary Animal Tests." Advanced Materials Research 535-537 (June 2012): 406–10. http://dx.doi.org/10.4028/www.scientific.net/amr.535-537.406.

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PU/ hydrogel composites or it containing fibroblast growth factor-2 (FGF-2) composites had been prepared by γ-ray radiation or room temperature(T) method in this paper. We utilized nanosized clay solution, thermosensitive N-isopropyl acrylamide (NIPAAm), Polyurethane (PU), and acrylic acid (AAc) as the starting materials to synthesize PU/hydrogel nanocomposite products. The dipping and weighing tests, Material Testing Machine (LLOYD) were employed to analyze the physical and mechanical properties differentially. In addition, the drug release and the wound closure in animal experiments were als

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