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1
Myakova, Natalya. "ELNIT Annual conference. Review." Scientific and Technical Libraries, no. 11 (November 1, 2016): 98–102. http://dx.doi.org/10.33186/1027-3689-2016-11-98-102.
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The annual conference (general meeting) of the members of the International Association of Users and Developers of Electronic Libraries and New Information Technologies held during the Second World Professional Forum “The Book. Culture. Education. Innovations” (“Crimea-2016”) (June 4-12, 2016, Sudak, Republic of Cromea, Russian Federation) is reviewed. Reports by ELNIT President and Executive Director are summarized. Resolutions of the general meeting of ELNIT Association members are discussed.
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Lin, Cheng-Jian, Chun-Hui Lin, and Shyh-Hau Wang. "Integrated Image Sensor and Light Convolutional Neural Network for Image Classification." Mathematical Problems in Engineering 2021 (March 16, 2021): 1–7. http://dx.doi.org/10.1155/2021/5573031.
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Deep learning has accomplished huge success in computer vision applications such as self-driving vehicles, facial recognition, and controlling robots. A growing need for deploying systems on resource-limited or resource-constrained environments such as smart cameras, autonomous vehicles, robots, smartphones, and smart wearable devices drives one of the current mainstream developments of convolutional neural networks: reducing model complexity but maintaining fine accuracy. In this study, the proposed efficient light convolutional neural network (ELNet) comprises three convolutional modules which perform ELNet using fewer computations, which is able to be implemented in resource-constrained hardware equipment. The classification task using CIFAR-10 and CIFAR-100 datasets was used to verify the model performance. According to the experimental results, ELNet reached 92.3% and 69%, respectively, in CIFAR-10 and CIFAR-100 datasets; moreover, ELNet effectively lowered the computational complexity and parameters required in comparison with other CNN architectures.
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King, Shirley V. "ELNET — the electronic library database system." Electronic Library 9, no. 2 (February 1991): 61–72. http://dx.doi.org/10.1108/eb045040.
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Myakova, Natalya. "Annual Conference (General Meeting) of ELNIT Association members. A review." Scientific and Technical Libraries, no. 11 (November 1, 2017): 61–64. http://dx.doi.org/10.33186/1027-3689-2017-11-61-64.
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The annual Conference (General Meeting) of the members of the International Association of Users and Developers of Electronic Libraries and New Information Technologies (ELNIT) held within the framework of the Third World Professional Forum “The Book. Culture. Education. Innovations” - “Crimea-2017” (June 3-11, 2017, Sudak, Republic of Crimea, Russian Federation) is reviewed. The speeches delivered by ELNIT Association President and Executive Director are highlighted. The resolutions adopted by the Association Conference are quoted.
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Al-Jawarneh, Abdullah S., Mohd Tahir Ismail, and Ahmad M. Awajan. "Elastic Net Regression and Empirical Mode Decomposition for Enhancing the Accuracy of the Model Selection." International Journal of Mathematical, Engineering and Management Sciences 6, no. 2 (April 1, 2021): 564–83. http://dx.doi.org/10.33889/ijmems.2021.6.2.034.
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Elastic net (ELNET) regression is a hybrid statistical technique used for regularizing and selecting necessary predictor variables that have a strong effect on the response variable and deal with multicollinearity problem when it exists between the predictor variables. The empirical mode decomposition (EMD) algorithm is used to decompose the nonstationary and nonlinear dataset into a finite set of orthogonal intrinsic mode function components and one residual component. This study mainly aims to apply the proposed ELNET-EMD method to determine the effect of the decomposition components of multivariate time-series predictors on the response variable and tackle the multicollinearity between the decomposition components to enhance the prediction accuracy for building a fitting model. A numerical experiment and a real data application are applied. Results show that the proposed ELNET-EMD method outperforms other existing methods by capable of identifying the decomposition components that have the most significance on the response variable despite the high correlation between the decomposition components and by improving the prediction accuracy.
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Paasio, Harri. "”Niin kauan kuin elät, et voi olla oppimatta”." Aikuiskasvatus 15, no. 3 (March 1, 1995): 221–24. http://dx.doi.org/10.33336/aik.92343.
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Houssiau, F. A., C. Vasconcelos, D. D’Cruz, G. D. Sebastiani, E. de Ramon Garrido, M. G. Danieli, D. Abramovicz, et al. "The 10-year follow-up data of the Euro-Lupus Nephritis Trial comparing low-dose and high-dose intravenous cyclophosphamide." Annals of the Rheumatic Diseases 69, no. 01 (January 20, 2009): 61–64. http://dx.doi.org/10.1136/ard.2008.102533.
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Objective:To update the follow-up of the Euro-Lupus Nephritis Trial (ELNT), a randomised prospective trial comparing low-dose (LD) and high-dose (HD) intravenous (IV) cyclophosphamide (CY) followed by azathioprine (AZA) as treatment for proliferative lupus nephritis.Patients and methods:Data for survival and kidney function were prospectively collected during a 10-year period for the 90 patients randomised in the ELNT, except in 6 lost to follow-up.Results:Death, sustained doubling of serum creatinine and end-stage renal disease rates did not differ between the LD and HD group (5/44 (11%) vs 2/46 (4%), 6/44 (14%) vs 5/46 (11%) and 2/44 (5%) vs 4/46 (9%), respectively) nor did mean serum creatinine, 24 h proteinuria and damage score at last follow-up. Most patients in both groups were still treated with glucocorticoids, other immunosuppressant agents and blood pressure lowering drugs. After 10 years of follow-up, the positive predictive value for a good outcome of an early drop in proteinuria in response to initial immunosuppressive therapy was confirmed.Conclusion:The data confirm that a LD IVCY regimen followed by AZA—the “Euro-Lupus regimen”—achieves good clinical results in the very long term.
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Westers, Theresia M., Eline MP Cremers, Uta Oelschlaegel, Ulrika Johansson, Peter Bettelheim, Birgit Witte, Matteo G. Della Porta, Wolfgang Kern, and Arjan van de Loosdrecht. "Diagnostic Application of Immunophenotypic Analysis of Erythroid Dysplasia in Myelodysplastic Syndromes. a Report on Behalf of the European Leukemianet-IMDS-Flow Cytometry Working Group." Blood 124, no. 21 (December 6, 2014): 1914. http://dx.doi.org/10.1182/blood.v124.21.1914.1914.
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Abstract Discriminating between cytopenia(s) due to myelodysplastic syndromes (MDS) and due to other (non-clonal) causes can be challenging, especially when dysplasia as assessed by cytomorphology is minimal, and when other MDS-specific features (such as ring sideroblasts or cytogenetic aberrations) are absent. Current recommendations for diagnosing MDS endorse flow cytometry (FC) as a valuable and informative diagnostic tool. Most FC protocols focus on analyzing the progenitor cells and the maturing myelomonocytic lineage. However, one of the most frequently observed symptoms in MDS is anemia, which is often associated with erythrodysplasia. Therefore, flow cytometric features of nucleated erythroid cells may complement current validated FC tools. The international, multicenter study within the European LeukemiaNet MDS-FC working group (ELNet-IMDS-Flow) reported herein focused on defining those erythroid parameters that enable discrimination of dyserythropoiesis associated with MDS from erythropoiesis in non-clonal cytopenias. This analysis was based on ELNet iMDS-flow guidelines for studying nucleated erythroid cells and their expression of CD117, CD71, CD36, CD235a and CD105. [Westers et al., Leukemia 2012] Nineteen centers (members of the ELNet-iMDS-flow) collected FC data on the erythroid lineage in mainly low grade MDS cases and pathological and normal controls. Bone marrow aspirates were taken after informed consent in accordance with the Declaration of Helsinki and local ethics committee approval. Data from a learning cohort were compared among MDS patients and controls; the results were validated in a separate cohort. The learning cohort comprised 685 cases and the validation cohort 352 cases; in total 191 normal controls, 443 pathological controls, and 403 MDS cases were included. The data revealed that the analysis of the expression pattern of CD71 and that of CD36 on erythroid cells in combination with the percentage of CD117+ erythroid progenitors provides the best discrimination between MDS and non-clonal cytopenia. The selected markers were used to build an FC erythroid dysplasia score which displayed a sensitivity of 59% (95% CI: 49-68%) and a specificity of 84% (95% CI: 77-89%). Of note, not every MDS case shows signs of erythrodysplasia by cytomorphology whereas some non-clonal conditions do. Evaluation of the results in the validation cohort displayed a specificity of 77% (95% CI: 29-50%) and a sensitivity of 39% (95% CI: 66-85%) for separating pathologic controls and MDS cases based on FC erythroid dysplasia. Most “FC-dysplastic” cases in the pathological control group involved reactive conditions and cytopenia associated with infections. The majority of the “FC-dysplastic” controls demonstrated abnormal CD71 expression, which argues against the application of single aberrancies to indicate dysplasia. Considering only the presence of multiple erythroid aberrancies as erythroid dysplasia by FC increased the specificity to 96% and 95% in the learning and validation cohorts, respectively; however, at the cost of a markedly reduced sensitivity (37% and 21%, respectively). Ultimately, analysis of the erythroid and myeloid lineages should be combined to increase both sensitivity and specificity. In summary, the defined erythroid marker combination may aid the diagnostic work-up of cytopenic cases with suspected MDS, particularly in combination with flow cytometric evaluation of the progenitor cells and maturing myelomonocytic lineage. This will be implemented in an upcoming multicenter data collection exercise within ELNet iMDS-flow. Disclosures Kern: MLL Munich Leukemia Laboratory: Employment, Equity Ownership. Loosdrecht:Celgene: Consultancy.
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Van de Loosdrecht, Arjan A., and Theresia M. Westers. "Flow Cytometric Immunophenotyping in Myelodysplasia: Discovery and Diagnosis." Blood 124, no. 21 (December 6, 2014): SCI—24—SCI—24. http://dx.doi.org/10.1182/blood.v124.21.sci-24.sci-24.
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Abstract Diagnosis of myelodysplastic syndromes (MDS) is based on the integration of results from diagnostic tools. Initial laboratory assessments in patients with suspected MDS comprise analysis of peripheral blood and bone marrow with the gold standard of cytomorphology, conventional cytogenetics and interphase fluorescence-in-situ hybridization (FISH). Single nucleotide polymorphism (SNP) array and next-generation sequencing (NGS) are emerging techniques. The pathological hallmark of MDS is dysplasia. Flow cytometry (FC) can identify aberrancies in antigen expression and differentiation patterns that are indicative of dysplasia. FC is regarded instrumental and now even recommended in the diagnostic work-up of (suspected) MDS, especially when dysplasia by cytomorphology is minimal and cytogenetics shows no (typical) abnormalities. No single FC marker has been identified that is specific for MDS. The WHO-2008 classification recommends the presence of three or more FC aberrancies as highly suggestive for MDS. Minimal requirements to analyze dysplasia by FC have been proposed by the European LeukemiaNet (ELNet) working group (ELNet iMDS-Flow. ELNet recommendations should enable a categorization of FC results in cytopenic patients as "normal", "suggestive of", or “high probability of” MDS. FC as a single technique is not sufficient for the diagnosis of MDS and should be part of an integrated diagnostic work-up. Within the ELNet-iMDS-Flow group, a score based on four cardinal parameters was validated in MDS patients with <5% blasts and non-clonal cytopenic controls. This diagnostic score comprises: a) SSC of granulocytes (reflecting granularity) as ratio to lymphocytes; b) percentage of CD34+ myeloid progenitors of nucleated cells; c) percentage of B cell progenitors within the total CD34+ compartment; and d) expression of CD45 on CD34+ myeloid progenitors as ratio to lymphocytes. An abnormal value for every single parameter is assigned 1 point; MDS is highly suggestive when a score of 2 or more is obtained. Sensitivity of this diagnostic score was 70% and specificity 92%. It was demonstrated that this score also separates distinct subgroups with respect to prognosis within IPSS-R risk groups. Most flow scores mainly incorporate markers that cover the myelomonocytic lineage, e.g. the flow cytometric scoring system (FCSS). The latter separates patients with no and mild-to-moderate dysplasia from those with severe dysplasia. The FCSS has recently been shown to add significantly in separating patients into low or high risk disease in the revised IPSS subgroups. Finally, FCSS originally designed as a prognostic score, but can also be applied as a diagnostic score as combined with the 4-parameter FC diagnostic score to further improve sensitivity and specificity. Flow profiles based on the myelomonocytic lineage may fail to recognize MDS patients that exclusively show erythroid and/or megakaryocytic dysplasia. Analysis of megakaryocytes is hampered by their paucity. Recent advances shows that when adding erythroid markers such as CD71, CD36 and CD117/CD105 may add significantly to the diagnostic score of MDS by FC. Interestingly, aberrancies in the myelomonocytic lineage have been shown in patients with solely erythroid dysplasia with impact on prognosis. In addition, aberrant FC profile of myeloid progenitors has been associated with high transfusion requirements and disease progression as well as with a short duration of response or even lack of response to growth factor and azacitidine treatment. In conclusion, FC is a useful tool in the diagnostic work-up of MDS. Further studies on the value of FC in diagnosis, prognosis and predicting response to treatment are ongoing, as well as correlation of FC results with data obtained by SNP and NGS within prospective multicenter clinical trials in low and high risk MDS. Disclosures Van de Loosdrecht: celgene: Honoraria, Research Funding; alexion: Research Funding.
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Marchenko, Tatyana A., Tatyana V. Izvekova, Andrey A. Gushchin, Vladimir I. Grinevich, and Elena A. Golovkina. "WATER QUALITY IN TRIBUTARIES OF VOLGA RIVER IN WATER AREA OF GORKY WATER-STORAGE BASIN." IZVESTIYA VYSSHIKH UCHEBNYKH ZAVEDENIY KHIMIYA KHIMICHESKAYA TEKHNOLOGIYA 59, no. 5 (July 12, 2018): 89. http://dx.doi.org/10.6060/tcct.20165905.5393.
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In article the results of complex study of water quality for tributaries of Volga river in a water area of Gorky water-storage basin. Study methods included both the chemical and biological methods of pollution level control. For all water flows the excess of MPC on heavy metals (by 1-27 times), partially on copper by 1-27, on iron –by 3-9, on manganese by 1-8, as well as on ammonium cations – by 1-6, and difficult-oxidizing organic compounds (COD)-by 2-4 is observed. It was established by the bio-testing method (test-objects are the chlorella algae and Daphnia) that water samples taken in the region of river mouths of Gorky water-storage basin (Sunzha, Kazokha, Elnat, Kineshemka) don’t posses acute toxicity but they are slightly toxicity.
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Faulwetter, Sarah, Panayiotis Gotsis, Sofia Reizopoulou, Sotiris Orfanidis, Theodoros Kevrekidis, Artemis Nicolaidou, Nomiki Simboura, et al. "ElNet: The Greek biodiversity transitional waters information system. An exemplar for the development of distributed information networks in Europe." Aquatic Conservation: Marine and Freshwater Ecosystems 18, S1 (August 19, 2008): S135—S142. http://dx.doi.org/10.1002/aqc.950.
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Oelschlaegel, Uta, Theresia M. Westers, Brigitte Mohr, Ulrika Johansson, Dolores Subira, Gerhard Ehninger, Martin Bornhäuser, Arjan A. van de Loosdrecht, and Uwe Platzbecker. "Flow Cytometric Response Monitoring in MDS with Del(5q) Using a Simple 5-Parameter-Score - an Elnet Imds-Flow Experience." Blood 128, no. 22 (December 2, 2016): 3179. http://dx.doi.org/10.1182/blood.v128.22.3179.3179.
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Abstract Recently, we have established a 5-parameter flow cytometry (FCM)-based score allowing for a precise prediction a deletion (5q) in therapy naïve MDS patients. The aim of this prospective study was to test, whether this FCM-based profiling is at least equal to the cytogenetics/FISH-based del(5q) detection for monitoring response to treatment. Overall, 229 FCM investigations were performed in 57 patients with MDS and del(5q) (IPSS-R very low/low: n=24, int: n119, high/very high n=22) including 39 patients with isolated del(5q) or one additional cytogenetic abnormality. The majority of analyses were performed in patients receiving lenalidomide or azacitidine (n=28 and n=21 patients), or in patients receiving chemotherapy and/or allogeneic transplantation (n=3), or growth factors (n=5). The del(5q)-FCM-score includes the following 5 parameters: myeloid progenitors (myPC > 2%) = 3 points, CD45 MFI ratio (lymphocytes: myPC ≤ 7.0) = 10, SSC ratio (granulocytes : lymphocytes < 6.0) = 2, CD71 (≤ 20%) on granulocytes = 1.5, and female gender = 1.5; a score ≥ 15.0 indicates the presence of del(5q). A standardized FCM (lyse-stain-wash) and cytogenetics/FISH were performed according to ELN guidelines at the TU of Dresden, VUMC of Amsterdam, UH of Bristol, and UH of Guadalajara. Before therapy, in 61 cytogenetic/FISH analyses in 40 MDS patients a del(5q) was detectable. In 53/561 (87%) FCM measurements, performed in parallel, a del(5q) could be predicted using the del(5q)-FCM-score. Monitoring response to treatment, a cytogenetic complete response (CCR) with the disappearance of del(5q) was accompanied by the disappearance of the typical del(5q)-FCM-profile in all of the 50 (19 patients) FCM measurements (sensitivity=100%). Otherwise, in patients without CCR the presence of del(5q) could be predicted by FCM in 96/118 measurements (specificity=81%). Aiming at a still higher specificity, in the following analyses we included only patients who presented with a typical del(5q)-FCM-score before therapy (33 patients; 54 measurements; median score=16.5) resulting in the above described sensitivity of 100% (32 measurements; median score=13.0) as well as a remarkably high specificity of 97% (59/61 measurements; median score=16.5) for response prediction. Next, we compared cytogenetics/FISH and del(5q)-FCM-score with further methods for response monitoring as cytomorphology, two well established diagnostic FCM scores: FCSS (flow cytometry scoring system; Wells et al. 2003) and the diagnostic score (Ogata et al. 2009), as well as the assessment of hematological improvement (HI). Thus, cytomorphology and FCSS, analyzing dyspoiesis of multiple cell lineages, showed a CR or disappearance of a MDS typical FCSS in less than the half of all investigations being in cytogenetic CR (sensitivity: 41% and 38%), but those two methods revealed a high specificity (98% and 97%). On the other side, the analysis of HI was high sensitive (91%) but not specific (49%). The use of the diagnostic Ogata score, considering almost only abnormalities of the myeloid progenitors, ended up with a slightly lower sensitivity (91%) and specificity (89%) as cytogenetics/FISH and the del(5q)-FCM-score. Finally, response monitoring using the del(5q) FCM-score allowed for a better separation of overall survival compared to cytogenetics/FISH (p=0.039 vs. p=0.1725). Flow cytometry-based detection of a del(5q)-specific immunophenotype is feasible and can serve as a rapid tool for response monitoring during treatment with disease-modifying drugs. At the moment, we test whether the better separation of the patients' survival curves applying the FCM-score holds true in a larger patient cohort to be analyzed within the ELNet iMDS-Flow working group. Disclosures Platzbecker: TEVA: Honoraria, Research Funding.
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Luiz da Cunha, Evandro. "יחִָיד en la Biblia Hebrea: Implicaciones cristológicas." Revista Theologika 32, no. 1 (November 9, 2017): 6–37. http://dx.doi.org/10.17162/rt.v32i1.902.
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En el presente artículo nos proponemos exponer lapragmática y la semántica de יחִָיד en el Tanaj. No es tareasencilla establecer bases semánticas seguras sobre una vozcomo esta de la que tenemos un corpus tan restringido detextos en los que ocurre. Para intentar iluminar algunos aspectosun tanto oscuros, se compara el texto griego de laBiblia Hebrea (BH), la LXX, con el Texto Masorético (TM).1Además de arrojar luz sobre el TM, la LXX fue el texto usadopor los primeros cristianos para elaborar su cristología y suconcepción de la Divinidad, tal como la encontramos en elNT donde אֶחָד y יחִָיד fueron traducidos por ἀγαπητός, εἷς yμόνος. Por esta razón hemos decidido tener en cuenta en ladiscusión el texto en griego de la LXX, como forma de ofrecermás elementos de ayuda para aquellos que desean reconstruirel recorrido semántico-teológico de .יחִָידLos comentarios que presentamos a continuación no abarcanuna exposición del texto en su totalidad sino tan solo delos aspectos lingüístico-literarios e históricos que puedandilucidar la semántica de יחִָיד . Las cuestiones de crítica textualsolo serán incluidas cuando resulten relevantes parauna mejor comprensión de la perícopa.
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Zverevich, V. V., Yu V. Sokolova, and Ya L. Shrayberg. "The First Scientific and Practical Conference “Letter and Digit: Libraries on the way to Digitalization” (“BiblioPiter–2020”). Review of Events." Scientific and Technical Libraries, no. 7 (September 4, 2020): 141–62. http://dx.doi.org/10.33186/1027-3689-2020-7-141-162.
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The analytic report on the First Scientific and Practical Conference “Letter and Digit: Libraries on the way to Digitalization” (“BiblioPiter–2020”) is presented. The Conference took place in St. Petersburg on February 17–19, 2020. The Conference was organized by: Russian National Public Library for Science & Technology, Russian State Library, National Library Association “Libraries of the Future” (NALF), and International Association of Users and Developers of Electronic Libraries and New Information Technologies (ELNIT). The goal of the Conference was to join to get her the efforts by libraries and other academic, information and research institutions in developing new concepts, methods and technologies of the library and information sector in contemporary digital environment. The NALF School and IRBIS School “Digital Technologies and Services at Libraries” took place within the Conference program. The papers by known scientists and professionals in library science, information science, bibliography and cultural studies were delivered. The Conference also comprised the round tables “Special Patron at the Library” and “Intellects and Library Logos”, master class on patent search, special event on the Open Science issues, as well as the presentation of the monograph “Copyright at Libraries, Research and Academic Institutions” by Andrey I. Zemskov and Yakov L. Shrayberg. Totally over 30 papers were presented and over 100 participants attended the Conference. Over 600 users watched live Internet broad casts of the Conference events. The video records of every Conference event, as well as that of IRBIS School and NALF Schools are available in full on the YouTube channel of the Russian National Public Library for Science & Technology. Power point presentations are available on NALF website. The decision was made to organize the Conference annually. It will be hold every year in St. Petersburg in mid-February.
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Oelschlaegel, Uta, Brigitte Mohr, Katja Sockel, Gerhard Ehninger, Martin Bornhäuser, and Uwe Platzbecker. "Implementation of Diagnostic Flow Cytometry Allows for the Dissection of Age Related Loss of Y Chromosome from Myelodysplastic Syndrome (MDS)." Blood 128, no. 22 (December 2, 2016): 5531. http://dx.doi.org/10.1182/blood.v128.22.5531.5531.
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Abstract Loss of Y chromosome (-Y) is described as an age related cytogenetic polymorphism as well as an abnormality associated with clonal diseases mainly Myelodysplastic Syndromes (MDS) reported in up to 15% of these patients. However, it often remains an uncertainty dissecting these two conditions especially in patients with mild dysplasia and a low percentage of abnormal metaphases. The aim of our study was to investigate whether flow cytometry (FCM) allows for a valid discrimination between MDS and age related presence of a loss of Y chromosome. Bone marrow aspirates of 46 patients, suspicious for MDS, with a median age of 76 years and a cytogenetically detected -Y as single abnormality (>75% threshold for assignment to MDS; Wiktor et al. 2011) were investigated. According to cytomorphology MDS could be diagnosed in 26 patients (IPSS-R verylow/low=22, intermediate=3, high/very high=1) and CMML in 6 patients. MDS was excluded in 14 patients (11 IDUS, 1 aplastic anemia, and 2 pts. with reactive changes only). Further routine diagnostic procedures included FCM according to WHO and ELNet iMDS-Flow criteria. Thus, FCM was performed using an 8-color antigen panel measured on a FACS-Canto II cytometer. MDS were defined according to the current FCM-scores developed by Wells et al. (normal/mild: 0-1, moderate: 2-3, or severe: > 4; Blood 2003) and Ogata et al. (low: 0-1, high: >1; Haematologica 2009). In case of an intermediate Wells-score only, we demanded cross lineage antigen expression in myeloid progenitors as criteria for MDS/CMML. The median percentage of abnormal metaphases presenting with -Y was 52% (range: 12%-100%). Applying the above mentioned threshold of 75% abnormal metaphases as criteria for the presence of MDS, only 14 patients (30%) could clearly be allocated to MDS. Because of the present diagnostic incertainty we performed a comprehensive FCM investigation, applying Wells- and Ogata-Score. Thus, in 26 patients (63%) MDS was likely applying FCM. Furthermore, 4 patients with a normal/mild Wells-score plus a low Ogata-score were considered as "non-MDS". Applying cytomorphology 70% (32/46) of all -Y patients have been diagnosed as MDS. Of note, MDS diagnosis could be made by FCM or cytomorphology in an equal extent in the groups of more vs. less of 75% cytogenetically abnormal metaphases: 64% vs. 63% and 86% vs. 63%, respectively. Finally, we searched for patients meeting FCM plus cytomorphologic MDS criteria. Of importance, even in the group with less abnormal metaphases 50% of the patients showed a concordant FCM and cytomorphologic assignment to MDS or non-MDS vs. 64% concordance in the high cytogenetically aberrant group. Patients with a loss of Y chromosome as single cytogenetic abnormality often meet FCM and cytomorphologic criteria for MDS diagnosis independent from the load of abnormal metaphases. This study demonstrates that FCM can significantly contribute to MDS diagnosis in this specific subgroup of patients with -Y and often only mild dysplasia. Disclosures No relevant conflicts of interest to declare.
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Cremers, Eline MP, Theresia M. Westers, Canan Alhan, Claudia Cali, Heleen A. Visser-Wisselaar, Dana A. Chitu, Marian JPL Stevens-Kroef, et al. "Erythroid Lineage Analysis By Flow Cytometry Is of Highly Additive Value for MDS Diagnosis: A Study on Behalf of the HOVON89 Study Group." Blood 124, no. 21 (December 6, 2014): 4667. http://dx.doi.org/10.1182/blood.v124.21.4667.4667.
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Abstract Flow cytometric analysis is a recommended tool in the diagnosis of MDS. It is used to underline the diagnosis based on dysplastic features by cytomorphology and typical cytogenetic abnormalities (gold standard). Current MDS flow cytometry (MDS-FC) scoring systems that evaluate the maturing myelo-/monocytic lineage reach a median sensitivity and specificity of approximately 71% and 93%. Since anemia is the most common feature in MDS, it was hypothesized that addition of in depth analysis of the erythroid lineage could increase the sensitivity of MDS-FC. We analyzed 176 bone marrow aspirates by MDS-FC, including 110 patients with MDS (low/int-1 risk) within a prospective multicenter clinical trial and 66 pathological controls (i.e. iron deficiency, iron incorporation disorders, vitamin B12 deficiency, cytopenia due to medication or chronic disease, aplastic anemia, and PNH). For erythroid lineage analysis, markers recommended by the ELNet iMDS-flow group including i.e. expression level of CD71 and CD36 and percentage of erythroid progenitors cells (CD117 positive or CD105 positive cells) were explored. Data from erythroid analysis were added to a previously described MDS-FC scoring system (Van de Loosdrecht JNCCN 2013). This scoring system combines the diagnostic MDS-FC score (Della Porta Haematologica 2012), analysis of myeloid progenitor cells, and of the granulocytic and monocytic cell compartment (Wells Blood 2003). At initial assessment the MDS-FC method categorized 75/110 MDS patients as ‘compatible with MDS’ (true positive), 23/110 as ‘minor MDS related aberrancies’, and 12/110 patients as ‘not compatible with MDS’ (false negative). Within the pathological control group: 45/66 were ‘not compatible with MDS’ (true negative), 20/66 showed ‘minor MDS related aberrancies’, and 1/66 ‘compatible with MDS’ (false positive). Calculated sensitivity and specificity of MDS-FC without taken into account erythroid analysis were 68% and 98%, respectively, in line with previous studies. Results of the erythroid FC analysis showed 82/110 MDS patients with clear dyserythropoiesis compared to 16/66 of the pathological controls. A strong correlation between presence of dyserythropoiesis by MDS-FC and the diagnosis of MDS as assessed by morphology was found (Pearson’s R=0.71; p<0.001). Results derived of the erythroid analysis were added to the MDS-FC scoring system. This resulted in: 94/110 MDS patients ‘compatible with MDS’, 13/110 patients with ‘minor MDS related aberrancies’, and 3/110 patients ‘not compatible with MDS’. Compared to 40/66 pathological controls that were ‘not compatible with MDS’, 23/66 that showed ‘minor MDS related aberrancies’, and 3/66 that were ‘compatible with MDS’. Although dyserythropoiesis by CM can be present in pathological controls, dyserythropoiesis by FC remained highly specific for MDS. Calculated sensitivity increased to 85%, with only a minor decline in specificity (95%). In conclusion, addition of the erythroid lineage analysis to a diagnostic MDS-FC score system led to an increased sensitivity, as validated within this prospective clinical trial. These data indicate that if translating to routine clinical practice, the erythroid integrated MDS-FC approach is highly instrumental in refinement of MDS diagnosis. Disclosures De Greef: Celgene: Consultancy. Van de Loosdrecht:celgene: Honoraria, Research Funding; alexion: Research Funding.
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Koestoer, Clara Krishanti, Aris Catur Bintoro, and Dwi Pudjonarko. "GANGGUAN MEMORI EPISODIK PADA EPILEPSI LOBUS TEMPORAL DAN NONTEMPORAL." Majalah Kedokteran Neurosains Perhimpunan Dokter Spesialis Saraf Indonesia 35, no. 1 (December 1, 2017). http://dx.doi.org/10.52386/neurona.v35i1.41.
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EPISODIC MEMORY DISORDER IN TEMPORAL AND NONTEMPORAL LOBE EPILEPSYABSTRACTIntroduction: Memory is the most defected cognitive function in neurological problems including epilepsy. Amnesia, refers to episodic memory impairment (EMI), which components are regulated by various areas related to brain lobes especially the temporal lobe. EMI represents a major cognitive component in temporal lobe epilepsy (TLE) but also encounter in focal epilepsy outside the temporal lobe.Aim: To prove the difference between EMI that occurs in TLE and nontemporal lobe epilepsy (NTLE) patients.Method: A cross-sectional study on epilepsy patients in Neurology Clinic of dr. Kariadi Hospital, Semarang from November 2016 until January 2017. Subjects were devided into TLE and NTLE group based on clinical semiology and electroencephalography (EEG). Interviews and questionnaires were done to the characteristics of subjects which can aggravate EMI, and memory tests was performed using Word List Memory Task, Word List Memory Recall, and Word List Memory Recognition. Statistical analysis using Chi-square test and multivariate logistic regression.Results: Subjects obtained were 43 respondents consisting of 21 TLE and 22 NTLE subjects. Significant difference of EMI occurrence was shown in TLE compared to NTLE, especially on delayed recall component. Patients with history of epilepsy >10 years tend to have a significant EMI, while patients with first seizure >10 years old do not tend to have EMI with or without other confounding factors.Discussion: There are differences in the occurrence of EMI on the TLE and NTLE independently, especially delayed recall.Keywords: Episodic memory impairment, temporal lobe epilepsyABSTRAKPendahuluan: Memori merupakan fungsi kognitif yang paling rentan terkena gangguan neurologis termasuk epilepsi. Salah satunya adalah amnesia, merujuk pada gangguan memori episodik (GME) yang komponennya diatur oleh berbagai area terkait lobus otak terutama lobus temporal. Gangguan memori episodik (GME) mewakili komponen kognitif utama pada epilepsi lobus temporal (ELT), namun juga tidak jarang ditemui pada epilepsi fokal di luar lobus temporal.Tujuan: Mengetahui perbedaan GME pada penderita epilepsi lobus temporal dengan epilepsi lobus nontemporal (ELNT).Metode: Penelitian potong lintang terhadap pasien epilepsi yang berobat ke Poli Saraf RSUP dr. Kariadi, Semarang pada bulan November 2016 hingga Januari 2017. Subjek dikelompokkan menjadi ENT dan ELNT berdasarkan gambaran semiologi klinis dan elektroensefalografi (EEG). Dilakukan wawancara dan pemakaian kuesioner terhadap karakteristik subjek yang dapat memperberat GME, serta tes memori menggunakan Word List Memory Task, Word List Memory Recall, dan Word List Memory Recognition. Analisis statistik menggunakan uji Chi-square dan uji multivariat dengan regresi logistik.Hasil: Didapatkan 43 subjek yang terdiri dari 21 subjek ELT dan 22 subjek ELNT. Didapatkan subjek yang mengalami GME pada ELT lebih banyak secara bermakna dibandingkan ELNT, terutama pada komponen delayed recall. Subjek yang menderita epilepsi >10 tahun cenderung mengalami GME secara bermakna, sedangkan subjek yang mengalami serangan awal pada usia >10 tahun lebih cenderung tidak mengalami GME, baik secara independen maupun bersama-sama dengan faktor perancu yang lain.Diskusi: Terdapat perbedaan terhadap terjadinya GME pada ELT dan ELNT secara independen, terutama pada komponen delayed recall.Kata kunci: Epilepsi lobus temporal, gangguan memori episodik