Academic literature on the topic 'Embryonic'

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Journal articles on the topic "Embryonic"

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Sharpe, N. G., D. G. Williams, and D. S. Latchman. "Regulated expression of the small nuclear ribonucleoprotein particle protein SmN in embryonic stem cell differentiation." Molecular and Cellular Biology 10, no. 12 (1990): 6817–20. http://dx.doi.org/10.1128/mcb.10.12.6817-6820.1990.

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The SmN protein is a component of small nuclear ribonucleoprotein particles and is closely related to the ubiquitous SmB and B' splicing proteins. It is expressed in a limited range of tissues and cell types, including several undifferentiated embryonal carcinoma cell lines and undifferentiated embryonic stem cells. The protein declines to undetectable levels when embryonal carcinoma or embryonic stem cells are induced to differentiate, producing primitive endoderm or parietal endoderm or yielding embryonal bodies. This decline is due to a corresponding decrease in the level of the SmN mRNA. T
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Sharpe, N. G., D. G. Williams, and D. S. Latchman. "Regulated expression of the small nuclear ribonucleoprotein particle protein SmN in embryonic stem cell differentiation." Molecular and Cellular Biology 10, no. 12 (1990): 6817–20. http://dx.doi.org/10.1128/mcb.10.12.6817.

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The SmN protein is a component of small nuclear ribonucleoprotein particles and is closely related to the ubiquitous SmB and B' splicing proteins. It is expressed in a limited range of tissues and cell types, including several undifferentiated embryonal carcinoma cell lines and undifferentiated embryonic stem cells. The protein declines to undetectable levels when embryonal carcinoma or embryonic stem cells are induced to differentiate, producing primitive endoderm or parietal endoderm or yielding embryonal bodies. This decline is due to a corresponding decrease in the level of the SmN mRNA. T
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Yuan, Jianbo, Yuehui Chao, and Liebao Han. "Uncovering a Phenomenon of Active Hormone Transcriptional Regulation during Early Somatic Embryogenesis in Medicago sativa." International Journal of Molecular Sciences 23, no. 15 (2022): 8633. http://dx.doi.org/10.3390/ijms23158633.

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Somatic embryogenesis (SE) is a developmental process in which somatic cells undergo dedifferentiation to become plant stem cells, and redifferentiation to become a whole embryo. SE is a prerequisite for molecular breeding and is an excellent platform to study cell development in the majority of plant species. However, the molecular mechanism involved in M. sativa somatic embryonic induction, embryonic and maturation is unclear. This study was designed to examine the differentially expressed genes (DEGs) and miRNA roles during somatic embryonic induction, embryonic and maturation. The cut coty
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Lav, R., R. Heera, and L. M. Cherian. "Decoding the ‘embryonic’ nature of embryonal rhabdomyosarcoma." Journal of Developmental Origins of Health and Disease 6, no. 3 (2015): 163–68. http://dx.doi.org/10.1017/s204017441500015x.

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Embryonal rhabdomyosarcoma is one of the major defined histologic variants of rhabdomyosarcoma that is mainly reported in children. The histologic appearance of this neoplastic entity recapitulates normal myogenesis. The tumor cells variably exhibit the different cellular phases of myogenesis ranging from undifferentiated mesenchymal cells to elongated myoblasts, multinucleated myotubes and differentiated muscle fibers. The carefully orchestrated embryonic signaling pathways that are involved in myogenesis, conceivably also result in the genesis of rhabdomyosarcoma; albeit as a corollary to an
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Dani, C., A. G. Smith, S. Dessolin, et al. "Differentiation of embryonic stem cells into adipocytes in vitro." Journal of Cell Science 110, no. 11 (1997): 1279–85. http://dx.doi.org/10.1242/jcs.110.11.1279.

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Embryonic stem cells, derived from the inner cell mass of murine blastocysts, can be maintained in a totipotent state in vitro. In appropriate conditions embryonic stem cells have been shown to differentiate in vitro into various derivatives of all three primary germ layers. We describe in this paper conditions to induce differentiation of embryonic stem cells reliably and at high efficiency into adipocytes. A prerequisite is to treat early developing embryonic stem cell-derived embryoid bodies with retinoic acid for a precise period of time. Retinoic acid could not be substituted by adipogeni
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El-Shabrawi, Hattem M., Hend E. Wahba, Ahmed M. Gabr, Shafik I. El-Morsy, Mohamed A. Saber, and Shawky A. Bekheet. "Improvement of Wax Oil Content of Embryonic Callus of Jojoba Using Gamma Radiation." Plant Tissue Culture and Biotechnology 29, no. 2 (2019): 207–17. http://dx.doi.org/10.3329/ptcb.v29i2.44509.

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Embryogenic callus was obtained from the leaf explants of jojoba (Simmondsia chinensis (Link) Schneider cultured on MS containing 0.5 mg/l NAA and 0.5 mg/l Kn. Growth of embryonic callus increased in 0.5 mg/l Kn + 6% sucrose. In order to improve oil content, the embryonic callus was exposed to different doses (5, 10 and 15 Kr) of gamma radiation. It was found that oil content of embryonic callus of jojoba increased 1.41% by exposing to 5 Kr gamma radiation. Also, size of oil bodies in embryonic callus irradiated with 5 Kr increased compared to control. Furthermore, production of the fatty acid
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Doetschman, Thomas C., Harald Eistetter, Margot Katz, Werner Schmidt, and Rolf Kemler. "The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium." Development 87, no. 1 (1985): 27–45. http://dx.doi.org/10.1242/dev.87.1.27.

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The in vitro developmental potential of mouse blastocyst-derived embryonic stem cell lines has been investigated. From 3 to 8 days of suspension culture the cells form complex embryoid bodies with endoderm, basal lamina, mesoderm and ectoderm. Many are morphologically similar to embryos of the 6- to 8-day egg-cylinder stage. From 8 to 10 days of culture about half of the embryoid bodies expand into large cystic structures containing alphafoetoprotein and transferrin, thus being analagous to the visceral yolk sac of the postimplantation embryo. Approximately one third of the cystic embryoid bod
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Pera, M. F., B. Reubinoff, and A. Trounson. "Human embryonic stem cells." Journal of Cell Science 113, no. 1 (2000): 5–10. http://dx.doi.org/10.1242/jcs.113.1.5.

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Embryonic stem (ES) cells are cells derived from the early embryo that can be propagated indefinitely in the primitive undifferentiated state while remaining pluripotent; they share these properties with embryonic germ (EG) cells. Candidate ES and EG cell lines from the human blastocyst and embryonic gonad can differentiate into multiple types of somatic cell. The phenotype of the blastocyst-derived cell lines is very similar to that of monkey ES cells and pluripotent human embryonal carcinoma cells, but differs from that of mouse ES cells or the human germ-cell-derived stem cells. Although ou
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Yasuda, Satoshi, Tetsuya Hasegawa, Tetsuji Hosono, et al. "AW551984: a novel regulator of cardiomyogenesis in pluripotent embryonic cells." Biochemical Journal 437, no. 2 (2011): 345–55. http://dx.doi.org/10.1042/bj20110520.

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An understanding of the mechanism that regulates the cardiac differentiation of pluripotent stem cells is necessary for the effective generation and expansion of cardiomyocytes as cell therapy products. In the present study, we have identified genes that modulate the cardiac differentiation of pluripotent embryonic cells. We isolated P19CL6 cell sublines that possess distinct properties in cardiomyogenesis and extracted 24 CMR (cardiomyogenesis-related candidate) genes correlated with cardiomyogenesis using a transcriptome analysis. Knockdown of the CMR genes by RNAi (RNA interference) reveale
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Kulinski, Tomasz M., M. Rita T. Casari, Philipp M. Guenzl, et al. "Imprinted expression in cystic embryoid bodies shows an embryonic and not an extra-embryonic pattern." Developmental Biology 402, no. 2 (2015): 291–305. http://dx.doi.org/10.1016/j.ydbio.2015.04.010.

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Dissertations / Theses on the topic "Embryonic"

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Montgomery, Sarah Lynn. "Impedance measurement system for embryonic stem cell and embryoid body cultures." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/24661.

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Salanga, Matthew Charles. "EMBRYONIC VASCULAR DEVELOPMENT." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/203435.

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The formation of the embryonic vasculature is essential for life. The components driving this process are well conserved across vertebrate species. At the core of vascular development is the specification of endothelial precursor cells from nascent mesoderm. Transcription factors of the ETS family are important regulators of endothelial specification. In this document we characterize the role of the ETS transcription factors, ETV2, during embryonic vascular development.Expression analysis shows that Etv2 is highly expressed in hematopoietic and endothelial precursor cells in the Xenopus embryo
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Bailleul, Richard. "Embryonic patterning of the avian skin : mathematical modelling of embryonic dynamics." Electronic Thesis or Diss., Sorbonne université, 2019. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2019SORUS022.pdf.

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Depuis la publication par Alan Turing de son article ‘The Chemical Basis of Morphogenesis” en 1952, une révolution moléculaire a eu lieu en biologie et les mathématiciens ont fourni un nombre croissant de cadres théoriques afin de modéliser des motifs observés dans la nature. Cette thèse a pour objectif d’unifier les récentes découvertes dans ces deux domaines en proposant un cadre théorique et des schémas de développement pour l’émergence des motifs cutanés aviaires par l’étude de la dynamique de formation du plumage dorsal. J'ai caractérisé l'apparition des primordiums de plumes dans le dos
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Nortjé, Nico. "The moral status of embryonic stem cell research in the South African context /." Link to the online version, 2007. http://hdl.handle.net/10019.1/1372.

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Harrison, Sarah Ellys. "Utilising embryonic and extra-embryonic stem cells to model early mammalian embryogenesis in vitro." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/275424.

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Successful mammalian development to term requires that embryonic and extra-embryonic tissues communicate and grow in coordination, to form the body. After implanting into the uterus, the mouse embryo is comprised of three cell lineages: first, the embryonic epiblast (EPI) that forms the embryo proper, second, the extra-embryonic ectoderm (ExE) which contributes to the foetal portion of the placenta, and third, the visceral endoderm (VE) that contributes to the yolk sac. These three tissues form a characteristic ‘egg-cylinder’ structure, which allows signals to be exchanged between them and set
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Fijnvandraat, Arnoldus Cornelis. "Embryonic stem cell-derived cardiomyocytes." [S.l. : Amsterdam : s.n.] ; Universiteit van Amsterdam [Host], 2003. http://dare.uva.nl/document/68354.

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Brochu, Richard. "Pacemaking in embryonic chick heart." Thesis, McGill University, 1990. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=59533.

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Experiments were conducted to determine the currents involved in the pacemaker activity of aggregates and single cells from the embryonic chick heart. Two microelectrode voltage clamp studies of embryonic chick ventricular heart cell aggregates revealed two time-dependent current components in the pacemaker range of potentials ($-$60 to $-$120 mV). Barium (Ba, 5 mM) blocked the more negatively activated time-dependent component unmasking a component which remained inwardly directed for hyperpolarizing steps beyond the potassium equilibrium potential (E$ sb{ rm K}$). This component, which was b
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McCluskey, Jane T. "Mechanisms of embryonic wound healing." Thesis, University of Oxford, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318851.

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Tata, M. A. J. "Vascular regulation of embryonic neurogenesis." Thesis, University College London (University of London), 2016. http://discovery.ucl.ac.uk/1531030/.

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Neural progenitor cells (NPCs) in the embryonic nervous system generate a large number and variety of neurons in a process known as neurogenesis. NPCs reside in a regulatory ‘niche’ that provides an extensive array of diverse signals, and loss of any one of these signals can deplete the pool of NPCs and therefore impair neural development. These niche signals are most commonly studied in the forebrain, where a complex array of cell divisions yields a set of diverse NPCs. In contrast, little is known on the role of niche signals in regulating the behaviour of NPCs in the hindbrain, the evolutio
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Losa, Llabata Marta. "Gene regulation in embryonic development." Thesis, University of Manchester, 2016. https://www.research.manchester.ac.uk/portal/en/theses/gene-regulation-in-embryonic-development(8a9efb79-1ca9-409e-89b9-9d66213e593f).html.

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Branchial arches (BAs) are a series of transient structures that develop on the ventro-lateral surface of the head in vertebrate embryos. BAs initially appear as a series of similar segments; as development proceeds each BA will contribute to different structures. Here, it was investigated the transcriptional mechanisms that instruct the different fates of the BAs in development. Initially, each BA contains a blood vessel, known as aortic arch (AA) artery, that connects the dorsal aorta with the heart. Remodelling of the AAs is crucial to form the adult heart circulation. This process leads to
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Books on the topic "Embryonic"

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Turksen, Kursad. Embryonic Stem Cells. Humana Press, 2001. http://dx.doi.org/10.1385/1592592414.

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Irina, Klimanskaya, and Lanza Robert, eds. Embryonic stem cells. Elsevier Academic Press, 2006.

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W, Masters J. R., Palsson Bernhard, and Thomson James A. Dr, eds. Embryonic stem cells. Springer, 2007.

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Chiu, Arlene, and Mahendra S. Rao. Human Embryonic Stem Cells. Humana Press, 2003. http://dx.doi.org/10.1385/1592594239.

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Kursad, Turksen. Embryonic Stem Cell Protocols. Humana Press, 2006. http://dx.doi.org/10.1385/1597450367.

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Kursad, Turksen. Embryonic Stem Cell Protocols. Humana Press, 2006. http://dx.doi.org/10.1385/1597450375.

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Turksen, Kursad, ed. Embryonic Stem Cell Protocols. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2437-1.

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Zavazava, Nicholas, ed. Embryonic Stem Cell Immunobiology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-478-4.

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Sullivan, Stephen, Chad A. Cowan, and Kevin Eggan, eds. Human Embryonic Stem Cells. John Wiley & Sons, Ltd, 2007. http://dx.doi.org/10.1002/9780470511619.

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Turksen, Kursad, ed. Embryonic Stem Cell Protocols. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-2954-2.

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Book chapters on the topic "Embryonic"

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Alhaddi, Hanan. "Embryonic." In Monarch: Resilience through Evolution. Productivity Press, 2025. https://doi.org/10.4324/9781003564133-8.

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Vleck, Carol M., and David Vleck. "Embryonic Energetics." In Avian Energetics and Nutritional Ecology. Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0425-8_12.

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Gillott, Cedric. "Embryonic Development." In Entomology. Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-017-4380-8_20.

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Giudice, Giovanni. "Embryonic Morphogenesis." In The Sea Urchin Embryo. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-70431-4_2.

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Dancygier, Henryk. "Embryonic Development." In Clinical Hepatology. Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-93842-2_1.

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Weis, Judith S. "Embryonic Development." In Physiological, Developmental and Behavioral Effects of Marine Pollution. Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-6949-6_6.

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Huneman, Philippe. "Embryonic Induction." In Encyclopedia of Systems Biology. Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_933.

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Punzo, Fred. "Embryonic Development." In Adaptations of Desert Organisms. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-662-04090-4_2.

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Playford, Richard. "Embryonic Persons." In Agency, Pregnancy and Persons. Routledge, 2022. http://dx.doi.org/10.4324/9781003181576-5.

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Dettlaff, Tatiana A., Anna S. Ginsburg, and Olga I. Schmalhausen. "Embryonic Development." In Sturgeon Fishes. Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77057-9_3.

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Conference papers on the topic "Embryonic"

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Suh, Junwoo, Jia Fan, Stephanie M. Ford, Michiko Watanabe, Andrew M. Rollins, and Michael W. Jenkins. "Multiscale 3D imaging of the embryonic heart." In Multiscale Imaging and Spectroscopy VI, edited by Alex J. Walsh, Darren M. Roblyer, and Paul J. Campagnola. SPIE, 2025. https://doi.org/10.1117/12.3044384.

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Musina, Guzel R., Deirdre M. Scully, Andrew L. Lopez, and Irina V. Larina. "Novel optogenetics tool for mouse embryonic cardiodynamic study." In Light in Cardiology 2025, edited by Gijs van Soest, Christos Bourantas, and Irina V. Larina. SPIE, 2025. https://doi.org/10.1117/12.3043948.

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Faubert, Andre C., and Shang Wang. "Assessing developmental biomechanics in the embryonic mouse heart." In Light in Cardiology 2025, edited by Gijs van Soest, Christos Bourantas, and Irina V. Larina. SPIE, 2025. https://doi.org/10.1117/12.3044104.

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Larina, Irina V. "Biophonic investigation of mouse embryonic cardiac development and dynamics." In Light in Cardiology 2025, edited by Gijs van Soest, Christos Bourantas, and Irina V. Larina. SPIE, 2025. https://doi.org/10.1117/12.3062986.

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Thoson, J. "Embryonic stem cell research." In 2006 IEEE Aerospace Conference. IEEE, 2006. http://dx.doi.org/10.1109/aero.2006.1655714.

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Zuser, E., J. Newmark, T. Chernenko, M. Diem, P. M. Champion, and L. D. Ziegler. "Non-invasive Imaging of Embryonic Stem Cells Differentiation via Formation of Embryoid Bodies." In XXII INTERNATIONAL CONFERENCE ON RAMAN SPECTROSCOPY. AIP, 2010. http://dx.doi.org/10.1063/1.3482805.

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Sabir, K., and D. Lowe. "Embryonic stream processing using morphogens." In 2010 Second World Congress on Nature and Biologically Inspired Computing (NaBIC 2010). IEEE, 2010. http://dx.doi.org/10.1109/nabic.2010.5716321.

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Rasala, Richard. "Embryonic object versus mature object." In the 8th annual conference. ACM Press, 2003. http://dx.doi.org/10.1145/961511.961538.

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Wan, Chen-rei, Seok Chung, Ryo Sudo, and Roger D. Kamm. "Induction of Cardiomyocyte Differentiation From Mouse Embryonic Stem Cells in a Confined Microfluidic Environment." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-203995.

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Embryonic stem cell derived cardiomyocytes are deemed an attractive treatment option for myocardial infarction. Their clinical efficacy, however, has not been unequivocally demonstrated. There is a need for better understanding and characterization of the cardiogenesis process. A microfluidic platform in vitro is used to dissect and better understand the differentiation process. Through this study, we find that while embryoid bodies (EBs) flatten out in a well plate system, differentiated EBs self-assemble into complex 3D structures. The beating regions of EBs are also different. Most beating
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Varner, Victor D., Dmitry A. Voronov, and Larry A. Taber. "Mechanics of Embryonic Head Fold Morphogenesis." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-193032.

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Head fold morphogenesis constitutes the first discernible epithelial folding event in the embryonic development of the chick. It arises at Hamburger and Hamilton (HH) stage 6 (approximately 24 hours into a 21-day incubation period) and establishes the anterior extent of the embryo [1]. At this stage, the embryonic blastoderm is composed of three germ layers (endoderm, mesoderm, and ectoderm), which are organized into a flat layered sheet that overlies the fibrous vitelline membrane (VM). Within this blastodermal sheet, a crescent-shaped head fold develops just anterior to the elongating notoch
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Reports on the topic "Embryonic"

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Weier, Jingly F., Christy Ferlatte, and Heinz-Ulli G. Weier. Somatic genomic variations in extra-embryonic tissues. Office of Scientific and Technical Information (OSTI), 2010. http://dx.doi.org/10.2172/1001041.

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พฤกษานานนท์, กำธร, รัฐจักร รังสิวิวัฒน์, ปราณี นำชัยศรีค้า, วิชชุดา อานนท์กิจพานิช та ประมวล วีรุตมเสน. เซลล์ต้นกำเนิดตัวอ่อน : รายงานวิจัย. จุฬาลงกรณ์มหาวิทยาลัย, 2008. https://doi.org/10.58837/chula.res.2008.24.

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เนื่องจากการใช้เซลล์พี่เลี้ยงที่แยกได้จากตัวอ่อนของหนู เพื่อเลี้ยงเซลล์ต้นกำเนิดตัวอ่อนมนุษย์ มีความเสี่ยงต่อการปนเปื้อนของเชื้อโรคที่มาจากหนู ดังนั้น การศึกษาวิจัยจึงมีจุดประสงค์เพื่อสร้างเซลล์ต้นกำเนิดตัวอ่อนมนุษย์โดยใช้เซลล์พี่เลี้ยงที่แยกได้จากคน เซลล์พี่เลี้ยงชนิดไฟโบรบลาสแยกได้จากผิวหนังบริเวณหน้าท้องของผู้หญิงที่เข้ารับการผ่าตัดทำคลอด ใช้ตัวอ่อนที่เกิดการปฎิสนธิภายนอกร่างกาย และผ่านการแช่แข็งจำนวนสิบตัวอ่อน เพื่อสร้างเซลล์ต้นกำเนิดตัวอ่อนมนุษย์กลุ่ม อินเนอร์เซลล์แมส (inner cell mass: ICM) ของตัวอ่อน 8 ตัวอ่อนถูกแยกออกจากเซลล์โทรเฟคโตเดิร์ม (trophectoderm: TE) โดยการใช้แท่งแก้วไปเปตขนาดเ
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Jessup, John. Inhibition of Embryonic Genes to Control Colorectal Cancer Metastasis. Defense Technical Information Center, 2012. http://dx.doi.org/10.21236/ada567286.

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Jessup, John M. Inhibition of Embryonic Genes to Control Colorectal Cancer Metastasis. Defense Technical Information Center, 2013. http://dx.doi.org/10.21236/ada595246.

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Jessup, John M. Inhibition of Embryonic Genes to Control Colorectal Cancer Metastasis. Defense Technical Information Center, 2014. http://dx.doi.org/10.21236/ada615207.

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Bushman, Wade. An Embryonic Growth Pathway is Reactivated in Human Prostate Cancer. Defense Technical Information Center, 2005. http://dx.doi.org/10.21236/ada442996.

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Harada, John J. Final Report for Regulation of Embryonic Development in Higher Plants. Office of Scientific and Technical Information (OSTI), 2013. http://dx.doi.org/10.2172/1097049.

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Bushman, Wade. An Embryonic Growth Pathway is Reactivated in Human Prostate Cancer. Defense Technical Information Center, 2003. http://dx.doi.org/10.21236/ada420333.

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Wong, Eric A., and Zehava Uni. Nutrition of the Developing Chick Embryo: Nutrient Uptake Systems of the Yolk Sac Membrane and Embryonic Intestine. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7697119.bard.

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Abstract:
We have examined the developmental changes in composition, amount, and uptake of yolk nutrients (fat, protein, water and carbohydrates) and the expression ofnutrient transporters in the yolk sac membrane (YSM) from embryonic day 11 (Ell) to 21 (E21) and small intestine from embryonic day 15 (E15) to E21 in embryos from young (22-25 wk) and old (45-50 wk) Cobb and Leghorn breeder flocks. The developmental expression profiles for the peptide transporter 1 (PepTl), the amino acid transporters, EAAT3, CAT-1 and BOAT, the sodium glucose transporter (SGLTl), the fructose transporter (GLUT5), the dig
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Stepanova, A. M. The use of probiotics in poultry industry since the embryonic period. Федеральное государственное бюджетное научное учреждение Федеральный научный центр - Всероссийский научно-исследовательский институт экспериментальной ветеринарии имени К.И. Скрябина и Я.Р. Коваленко Российской академии наук, 2018. http://dx.doi.org/10.18411/lj978-20182-363367.

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