Dissertations / Theses: 'EN 16516'

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Fritz, Michael. "Mechanismen des radialen Volumenflusses und der radialen Permeation von Osmolyten in verzweigten Wurzeln junger Maispflanzen (Zea mays L.) und halmbürtigen Adventivwurzeln des Schilfes (Phragmites australis Trin. ex Steudel)." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16516.

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Der radiale Wasserfluss durch die feinen Seitenwurzeln von Schilf- und Mais ist vom radialen Teilchenfluss entkoppelt. Der radiale Wasserfluss wird bereits im Kortex der Wurzel durch den Protoplasten kontrolliert, da die Strömung auf dem apoplastischen Zellwandweg um die Protoplasten herum gegenüber der Strömung durch die Protoplasten nicht signifikant ist. Der radiale Reflexionskoeffizient der Wurzeln wird durch den Reflexionskoeffizient der Plasmamembran bestimmt. Die Feinwurzeln von Schilf- und Mais besitzen einen Reflexionskoeffizienten für Salze, Zucker, Zuckeralkohole und Polymere der sich nicht signifikant von eins unterscheidet. An intakten Wurzeln wurde dies durch die Abwesenheit von solvent drag für NaCl und Mannitol bei der Steigerung des Wasserflusses und der gleich großen hydraulischen Wirkung von osmotischen und hydrostatischen Kräften auf die Exsudation nachgewiesen. Die radialen Wände der Endodermis von Schilf- und Maiswurzeln sind keine perfekte Diffusionsbarriere. Liegen die genannten Stoffe in einer signifikanten Konzentration in der Zellwand vor permeieren sie passiv unter Umgehung der Protoplasten durch die Endodermis in die Xylemgefäße. Auch die Epidermis/Hypodermis der untersuchten Wurzeln hat die Eigenschaft einer semipermeablen Membran in der osmotische Druckgradienten einen Volumenfluss erzeugen. Es wurden zwei Methoden etabliert, mit denen sich der osmotische Druck des Xylemsaftes in isolierten Feinwurzeln bestimmen lässt. Die Feinwurzeln unterschieden sich hinsichtlich des osmotischen Druckes ihres Xylemsaftes und ihrer radialen hydraulischen Leitfähigkeit stark. Die bekannte Fähigkeit der Schilfpflanzen Natriumionen an der Sprossbasis aus dem Xylem zu eliminieren muss um Chloridionen erweitert werden. Die hohe Permeabilität der Endodermis für NaCl verringert die osmotische Wirkung des Brackwassers auf die Wasseraufnahme. Die Entkopplung der Salzaufnahme vom Wasserfluss vermeidet eine exzessive Salzbelastung des Sprosses. Radial Water fluxes are not coupled to the radial solute fluxes in fine lateral roots of mays and reed. The radial water flow is already controlled by the protoplast in the cortical parenchyma as the hydraulic conductivity of the cell wall path circumventing the protoplasts is negligible compared to hydraulic conductivity of the pathway through the protoplast. The radial reflection coefficient of the root is defined by the reflection coefficient of the plasma membrane. Therefore fine laterals of the common reed (Phragmites australis) and maize (Zea mays) therefore exhibit a reflection coefficient for salts, sugars, alditols and polymers that is not significantly different from unity. This conclusion was drawn from the absence of solvent drag for NaCl and mannitol with increasing water flux and by the observation of equality of the hydraulic effect of both osmotic and hydrostatic forces on the exudation flow in intact roots of both plants. The radial walls of the endodermis are no absolute barrier for diffusion of small osmolytes. In the presence of high cell wall concentrations, the abovementioned osmolytes passively permeated into the xylem vessels at high rates circumventing the protoplast. The epidermis/hypodermis exhibits a semipermeable barrier as well wherein osmotic forces can create a radial volume flux. Two methods were established that allow for the determination of the flow direction and the osmotic pressure of the xylem sap in isolated fine laterals. Laterals differed strongly regarding their hydraulic conductivity and the osmotic pressure of their xylem sap. The known ability of the reed plant to remove sodium ions from the ascending sap has to be expanded for chloride. The high permeability of the endodermis for NaCl reduces the osmotic force of the brackish medium on water uptake. Uncoupling of radial water from the solute fluxes avoids the excessive permeation of NaCl and its accumulation in the assimilating leaves at high rates of transpiration.

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Pastorino, Chiara. "L’Unione Sovietica attraverso le canzoni popolari. Proposte di traduzione di canzoni sulla guerra come porta tra passato e presente." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/16516/.

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Il presente elaborato ha come scopo quello di fornire una panoramica del quadro storico e sociale dell’Unione Sovietica nel periodo compreso tra il 1915 e il 1975, per mezzo della traduzione dei testi di alcune canzoni popolari da me selezionate. Nella mia scelta ho tenuto conto di vari aspetti: l’anno di composizione, la notorietà della canzone in Russia e nel resto del mondo, la musicalità e soprattutto il contenuto dei testi. La disposizione in ordine cronologico ha lo scopo di illustrare come i cambiamenti storici abbiano influenzato la musica popolare, le necessità che questa doveva soddisfare, la percezione che trasmetteva e le reazioni che provocava. Nell’analisi ho cercato di ricoprire varie fasi e aspetti tipici del conflitto bellico, concretizzandoli in avvenimenti specifici della Guerra Civile russa e della Grande Guerra Patriottica. E soprattutto ho cercato di trasmettere la mia passione per la lingua e la cultura di questo meraviglioso Paese e per la sua musica tradizionale.

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Biggins, Rebecca Margaret. "Marketing heritage tourism destinations : community and commercial representations of the past : a collective case study investigation of Yorkshire and Huelva." Thesis, University of Leeds, 2016. http://etheses.whiterose.ac.uk/16516/.

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The cultural practice of heritage is naturally and prominently about people. It is undeniably people who create, select, share, contest and construct heritage each and every day. Yet the hegemonic discourse of heritage currently disengages people from their past and reconstitutes our interactions with this past. The material realities of heritage are now selected, contested and represented for communities, by someone else. The authority of expertise and ancestry defines places for people, rather than defining those places with the people to whom they naturally belong, their communities. As such the topic of community engagement is increasingly important. As such this study identifies a clear and concerning dissonance between commercial and community views and perceptions of heritage in the destinations of Yorkshire and Huelva province. The purpose of this study was to identify any dissonance between the community and commercial voices behind heritage tourism destinations and any possible agency this has. The cross-cultural, qualitative and interpretivist approach to this research identified several consequences of the exclusion of these community voices when marketing and branding heritage tourism destinations. The outcome is a widening ideological gap between these two stakeholder groups. Consequently, the brand identity and destination product suffer as the value present in destination communities is not harnessed. This thesis argues that a greater understanding of the value of the community voice is required, and that heritage communities need to be included in the heritage destination marketing process. The findings of the thesis demonstrate that the dissonance between the community and commercial case study destinations studied here has direct implications upon both the community and commercial stakeholders of the destinations. The commercial implications are found to surround; word of mouth, friends and family, positive interactions and tourist perceptions. For the local community the agency of the dissonance has implications regarding the place attachment, place identity and place dependence. From the findings the model entitled “the key steps for community empowerment and engagement throughout the heritage destination marketing process” (Figure 21) has been developed. This was applied in the destination of Triguerors Andalucía, and is the central recommendation of this study for practitioners to apply and academics to study further.

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Liang, Shu-Yuan. "Opioid-taking self-efficacy in Taiwanese Outpatients with cancer pain." Queensland University of Technology, 2007. http://eprints.qut.edu.au/16516/.

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Despite the fact that as many as 80-90% of patients with cancer pain can be effectively treated using pharmacological therapies and other advanced approaches, 31% to 85% of cancer patients in Taiwan still experience varying levels of pain. Pain is one of the symptoms that patients fear most; it overwhelms all aspects of patients' lives and creates a sense of uncertainly and hopelessness. Pain control is, therefore, a high priority in the treatment of cancer patients. Pharmacological therapy is the cornerstone of cancer pain management. With the current trend toward outpatient care, many patients are being required to assume greater responsibility for self-management of prescribed analgesics at home to deal with the variable and complex nature of cancer pain and side effects of opioids. Patients however, have misconceptions regarding analgesics and a series of difficulties when attempting to put a pain management regimen into practice. This research addressed the hypothesis that self-efficacy beliefs might play an important role in analgesic adherence and pain experience in Taiwanese outpatients with cancer. The purpose of this study was to develop a scale to measure the self-efficacy expectations relating to opioid-taking in Taiwanese outpatients with cancer. Another purpose was to explore how opioid-taking self-efficacy and beliefs about opioid analgesics contribute to patients' analgesic adherence and pain experience in Taiwanese outpatients with cancer. In the first stage semi-structured interviews were conducted to collect data from a purposeful sample (n=10) of oncology outpatients from two teaching hospitals in the Taipei area of Taiwan. The purpose of this phase was to identify behaviours and situational impediments associated with analgesic taking. Findings from this phase were used to develop a scale to measure opioid-taking self-efficacy. In the second stage a pilot test with a convenience sample (n=30) was conducted to test the validity and reliability of the new scale and to identify the feasibility of using the scale in a cross-sectional survey. In the third stage a cross-sectional survey was undertaken (n=92) to describe pain experiences, analgesic adherence, beliefs about opioid analgesics, and opioid-taking self-efficacy in Taiwanese outpatients with cancer and to explore how opioid-taking self-efficacy and beliefs about opioid analgesics contributed to analgesic adherence and pain experience. Results of this study highlight an important issue - under-treatment of cancer pain in this group of Taiwanese outpatients. As well, low adherence rates to opioid analgesics in cancer outpatients arose as an important issue in this study. A range of misconceptions about using opioids for pain was also common amongst the sample. Despite these misconceptions, patients reported being moderately confident in their ability to perform self-management behaviours related to their prescribed opioid-taking. Results of this research supported the notion that patients' self-efficacy in relation to taking their prescribed opioid regimen was a significant independent predictor of patients' adherence behaviour and pain relief, but not of pain severity. Beliefs about opioid analgesics were also an independent predictor of patients' adherence, but not of pain relief or pain severity. In addition, findings from this study provided support for the validity and reliability of the opioid-taking self-efficacy scale. Results suggested there is a need for systematic assessment of beliefs affecting patients' opioid adherence behaviours for cancer pain control, including perceived personal self-efficacy and beliefs about opioid analgesics. Educational programs that focus on overcoming patients' misconceptions (beliefs) about taking opioid analgesics may be particularly beneficial. In addition, this study advocates that conducting self-efficacy-enhancing interventions may improve medication adherence for patients and therefore pain relief. More research is needed to demonstrate the construct validity of the self-efficacy scale and to evaluate self-efficacy enhancing interventions in cancer pain management.

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OSONO, Takashi, Koichi ITO, Masayo MINAMI та ін. "14C測定による粗大枯死材の枯死年および分解速度の推定". 名古屋大学年代測定資料研究センター, 2011. http://hdl.handle.net/2237/16516.

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Govender, Selvakumaran. "Bioaffinity separation using ligand-modified pluronic and synthetic membranes." Thesis, Stellenbosch : University of Stellenbosch, 2011. http://hdl.handle.net/10019.1/16516.

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Thesis (PhD)--University of Stellenbosch, 2005. ENGLISH ABSTRACT: A new membrane based affinity separation system that is bio-specific, biocompatible, well characterised and capable of being regenerated or re-used is described. The amphiphilic non-ionic surfactant Pluronic® F108, was covalently derivatised to form two novel bioligands (Pluronic-Biotin and Pluronic-DMDDO) for the bio-specific immobilisation of avidin conjugated proteins and histidine tagged proteins respectively. Pluronic was also used to non-covalently functionalise nonporous membranes for ligand attachment and to simultaneously shield the surfaces from non-specific protein adsorption. Each component of this bioaffinity system (from the membrane matrix to the elution/desorption of the ligate/ligand system) was studied with the aim of producing a well characterised system and key quantitative data for the development of a robust, reliable, re-usable and scalable technology. Specifically, this study describes: 1. The fabrication and partial characterisation of nonporous planar and capillary membranes as model affinity matrices. 2. The development and evaluation of a robust protocol for solvent desorption and accurate colorimetric quantification of Pluronic® F108 and its derivatives. 3. Interfacial analysis of Pluronic adsorption onto nonporous affinity membranes, including the direct solid-state analysis of model, halogenated Pluronic derivatives using nuclear microprobe analysis. 4. Development of a surfactant based protocol for affinity membrane regeneration and re-use. 5. Specific bioaffinity immobilisation of avidin conjugated peroxidase onto biotinylated membranes in the presence of model protein foulants. 6. Cloning and expression of C-terminal hex-histidine tagged human cytochrome b5 into the bacterial expression system E. coli BL-21 DE3. 7. Development and characterisation of an immobilised metal affinity membrane system for metal chelation (Ni2+, Cu2+ and Zn2+) using a new chelator Pluronic- N,N-dicarboxymethyl-3,6-diazaoctanedioate and the bio-specific immobilisation of N-terminal hex-histidine tagged pantothenate kinase. AFRIKAANSE OPSOMMING: 'n Nuwe membraan-gebaseerde affiniteitskeidingsisteem word beskryf wat biospesifiek, bioversoenbaar en goed gekarakteriseer is, en geregenereer of hergebruik kan word. Die amfifiliese nie-ioniese surfaktant Pluronic is kovalent gederivatiseer om twee nuwe bioligande (Pluronic-Biotien en Pluronic-DMDDO) te vorm vir biospesifieke immobilisering van proteïnligate. Pluronic is ook gebruik om nie-poreuse membrane niekovalent te funksionaliseer vir ligandaanhegting en om hulle oppervlaktes teen niespesifieke proteïen-adsorbsie af te skerm. Elke komponent van hierdie bioaffiniteitsisteem (van die membraanmatriks tot die uitwas/desorpsie van die ligaat/ligand sisteem) is ondersoek met die doel om 'n goed-gekarakteriseerde sisteem te produseer en om kwantitatiewe data te genereer vir die ontwikkeling van 'n robuuste, betroubare, herbruikbare en opskaleerbare tegnologie. Hierdie studie beskryf spesifiek: 1. Die fabrisering en gedeeltelike karakterisering van nie-poreuse planêre en kapillêre membrane as model affiniteitsmatrikse. 2. Die ontwikkeling en evaluering van 'n robuuste protokol vir oplosmiddel desorpsie en akkurate kolorimetriese kwantifikasie van Pluronic® F108 en afgeleides daarvan. 3. Intervlakanalises van Pluronic adsorpsie op nie-poreuse affiniteitsmembrane, insluitend die direkte vastetoestand analise van model ligand-gemodifiseerde Pluronic deur die gebruik van kern-mikrosonde analise. 4. Ontwikkeling van 'n surfaktant-gebaseerde protokol vir affiniteitsmembraan regenerering en hergebruik. 5. Spesifieke bioaffiniteitsimmobilisering van avidien-gekonjugeerde peroksidase op gebiotinileerde membrane in die teenwoordigheid van model bevuilende proteïne. 6. Klonering en uitdrukking van C-terminaal hex-histidien geëtiketeerde menslike sitochroom b5 in die bakteriële uitdrukkingsisteem E. coli BL-21 DE3. 7. Ontwikkeling en karakterisering van 'n geïmmobiliseerde metaalaffiniteitsmembraansisteem vir metaalchelering (Ni2+, Cu2+ en Zn2+) met behulp van die nuwe cheleerder Pluronic-N,N-dikarboksimetiel-3,6- diasaoktaandioaat en die bio-spesifieke immobilisering van N-terminaal hexhistidiengeëtiketerde pantotenaatkinase.

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Ribeiro, João Manuel Gonçalves. "Internationalisation strategies : a sector analysis of the pharmaceutical industry." Master's thesis, Instituto Superior de Economia e Gestão, 2018. http://hdl.handle.net/10400.5/16516.

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Mestrado em Economia Internacional e Estudos Europeus A realização deste trabalho teve como intuito explicar o processo de internacionalização das empresas do sector da indústria farmacêutica. Para isso, foi feita, primeiramente, uma revisão extensiva da literatura sobre as estratégias de internacionalização. Com base nesta pesquisa, definiram-se as questões de investigação e hipóteses, que foram, posteriormente, testadas através da realização de um questionário. A análise focou-se nos diferentes comportamentos observados e nas razões apontadas pelas empresas para tomarem as decisões relacionadas com a internacionalização. As principais conclusões foram: - As empresas seguem modos de entrada (modelos de negócio) diferentes para diferentes países ou regiões. - Entre as principais teorias de internacionalização, o paradigma de OLI parece ser o que melhor reflete o processo de internacionalização da indústria farmacêutica. - Na decisão para onde internacionalizar, as empresas tendem a optar por mercados onde já tenham redes de contactos estabelecidos. - Durante a última crise financeira internacional (2008 - 2013) as empresas tomaram decisões de internacionalizar ou aumentar o grau de internacionalização. The purpose of this research is to develop a greater understanding of the internationalisation strategies followed by the companies operating in the pharmaceutical sector. This study reviewed and applied a range of relevant literature that was used to develop the research questions and hypotheses that were then empirically tested by the analysis of a questionnaire made to pharmaceutical companies. The analysis focused on the different behaviours and drivers of the companies in their internationalisation strategies. And the main results are: - Companies follow distinct entry mode for different countries and regions. - Among the traditional theories, the OLI paradigm is the model that better explains the internationalisation of the pharmaceutical companies. - On their decision of where to internationalise the companies tend to go to markets where they have established networks. - During the last financial crisis (2008 ? 2013) the companies took the decision to internationalise or increase the degree of internationalisation. info:eu-repo/semantics/publishedVersion

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Curay, Cabanillas Joao José. "Helmintiasis en vicuñas (Vicugna vicugna) en el distrito de Contumazá, departamento de Contumazá - Cajamarca." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2018. https://hdl.handle.net/20.500.12672/16516.

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El objetivo del estudio fue estimar la prevalencia y cargas de helmintos gastrointestinales en vicuñas en el distrito de Contumazá, provincia de Contumazá – Cajamarca, así como determinar su asociación con las variables edad y sexo e identificar los géneros parasitarios presentes en dicha comunidad. Se colectaron un total de 208 muestras de heces durante el Chaccu realizado en la época de esquila anual (agosto) del 2015. Las muestras fueron colectadas directamente del recto y colocadas en bolsa de polietileno adecuadamente rotuladas con la identificación correspondiente, las mismas que fueron colocadas en cajas térmicas junto con refrigerantes para su transporte hasta el laboratorio de Microbiología y Parasitología, sección Parasitología de la Facultad de Medicina Veterinaria, donde se procedió a realizar la técnica flotación con Solución de Sheather (solución saturada de azúcar) y sedimentación espontanea, para detectar la presencia de huevos de helmintos y de Fasciola hepatica respectivamente, además de estimar la carga parasitaria (hpg) con el método de McMaster modificado e identificación de larvas de nematodos mediante el cultivo de larvas y la técnica de Baermann. No se detectó la presencia de huevos de Fasciola hepatica en las muestras procesadas. Se obtuvo una prevalencia de helmintos de 81.3% en vicuñas. Las prevalencias de huevos de helmintos fueron de 61.1, 39.4, 26.9, 16.8, 8.7%, correspondiente a huevos tipo Strongylus, Nematodirus, Trichuris, Capillaria y Moniezia respectivamente. Los géneros de la familia Trichostrongylidae identificados en cultivos de larvas fueron Cooperia, Trichostrongylus, Ostertagia, Oesophagostomum, Haemonchus y Bunostomum. No se encontró diferencia significativa (p<0.05) entre las variables sexo, sin embargo, hubo asociación significativa con la variable edad. La carga parasitaria promedio de huevos de nematodos vario entre 103.8 a 121.3 huevos por gramo de heces, correspondiendo a una carga leve.

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Hermosilha, Helder Jorge Tareco. "Caracterização das propriedades elásticas do subsolo." Master's thesis, Universidade de Aveiro, 2004. http://hdl.handle.net/10773/16516.

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Mestrado em Minerais e Rochas Industriais A caracterização das propriedades elásticas do subsolo pode ser efectuada por métodos sísmicos para apoiar estudos do subsolo no âmbito da engenharia geotécnica e geológica. Este trabalho incidiu sobre métodos apoiados na geração/propagação/recepção de ondas P (compressionais) e ondas S (corte), em ensaios de campo. O trabalho de síntese revela o estado da arte da prospecção sísmica a pequena profundidade e a experimentação realizada, permite identificar e ultrapassar um conjunto de problemas associados a metodologias de colheita e processamento de dados, de refracção e reflexão sísmica. The characterization of the elastic properties of the sub-soil can be done using seismic methods in order to support sub-soil studies in the extent of geological and geotechnical engineering. This work happened on leaning methods in the generation/propagation/reception of P waves (compressional) and S waves (shear) in field rehearsals. The synthesis work reveals the state of the art of shallow seismic prospecting and the accomplished experimentation allows to identify and to overstep a group of problems associated to data acquisition and processing methodologies from seismic refraction to reflection.

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Chew, Jamie Kok-Wai. "Management of neovascular age-related macular degeneration with ranibizumab: Long-term outcomes and second eye outcomes." Thesis, The University of Sydney, 2016. http://hdl.handle.net/2123/16516.

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Background: Intravitreal anti-vascular endothelial growth factor (anti-VEGF) agents are the established standard of care for neovascular age related macular degeneration (nAMD), however there are currently limited data on long-term outcomes of this therapy. Ranibizumab is one such anti-VEGF agent administered to treat nAMD. Patients diagnosed with nAMD undergo regular clinic based follow-up as part of their treatment, often on a monthly basis. Assessment during these appointments includes optical coherence tomography (OCT) scans, which can contribute to the detection of nAMD in the second eye. There is limited data on the symptomatic status, clinical presentation and outcomes of second eye nAMD whilst undergoing regular assessment for the first treatment eye under these conditions. Aims: The first aim of this thesis is to evaluate the long-term (5-year) outcomes of intravitreal ranibizumab (an anti-VEGF agent) in treating nAMD by examining a cohort within a real life clinic setting. The second aim is to compare the clinical presentation and treatment outcomes between the first and second treated eyes in patients that developed nAMD in both eyes, whilst under regular review for unilateral nAMD. Methods: A total of 208 patients (208 eyes) were included in a retrospective case series assessing the 5-year outcomes of nAMD treated with ranibizumab, entitled the long-term ranibizumab study (LTRS) (Chapter 3). Intervention was an individualised treatment model after three initial monthly loading doses. Visual acuity (VA), central macular thickness (CMT), qualitative OCT features, and adverse events (AE) were determined for each visit. Snellen VA was converted to Early Treatment Diabetic Retinopathy Study (ETDRS) letters for analysis. To assess outcomes of second eyes diagnosed with nAMD, a retrospective case series entitled second-eye ranibizumab study (SERS) forms the second part of this thesis (Chapter 4). Forty-five consecutive patients fulfilled the inclusion criteria of commencing treatment with ranibizumab in the first eye for nAMD between July 2007 and March 2011,and subsequently developing nAMD in the second eye with at least 12-months of follow-up in each eye. Treatment was administered under the same conditions as the LTRS. Snellen VA was measured, and OCT examination of both eyes at each visit assessed the presence of intra-retinal fluid (IRF) and sub-retinal fluid (SRF). Patient reported symptoms were recorded at every clinic visit. Paired t-tests were used to assess changes in VA and CMT over the study duration of the LTRS and SERS and two sample t- tests were used to evaluate VA differences between groups. Changes in VA compared to baseline were classified into the three categories: stable VA (loss or gain of ≤15 letters), improved VA (gain of >15 letters), or worse VA (loss of >15 letters). Linear regression was used to assess the effects of age, gender, number of injections, previous treatment, medical history, medications, and baseline VA on both VA and CMT changes. Chi-square test or Fisher’s exact test were used to measure proportions of patients with visual stability and OCT fluid free status at 12-months in the SERS. Results: In the LTRS, mean VA improved by 1.9 letters after 1 year (p=0.020) and decreased by 2.4 letters over 5-years of the treatment (p=0.040). At the end of year 5, 11.1% (23/208) of patients improved VA by more than 15 letters and 68.8% (143/208) of patients had stable VA, while 20.2% (42/208) patients lost more than 15 letters. Patients with VA less than 35 letters (approximate Snellen VA 6/60) at baseline showed significant VA improvement after 5-years of treatment (mean increase 11.5 letters, p=0.01), whilst those that were between 70 and 85 letters (approximate Snellen VA 6/12 to 6/6) at baseline showed a mean decrease (-12.9 letters, p=<0.001). There was a positive relationship between injection numbers and VA improvement over the 5-years after adjusting for age and baseline VA (Regression coefficient 0.3, P<0.001). Mean CMT decreased by 28.3μm (p<0.01) over 5-years. Ocular AE, ocular serious adverse events (SAE) and systemic SAE occurred in 4.6%, 0.48% and 2%, respectively, during the follow-up period. Thirty-six per cent of patients did not require an injection in their 5th year of follow-up, whilst 24% of patients required near monthly (10-12) injections in the 5th year. In the SERS, second treated eyes commencing treatment with good VA at baseline (defined as >76 letters, or Snellen VA approximately 6/9)) showed greater stability of vision at 12-months vs. first treated eyes (p=0.05). There was no significant difference in mean VA change between first and second treated eyes. The proportion of OCT - fluid free eyes was higher amongst second treated eyes compared with first treated eyes at 12-months (70% vs. 40%, p=0.02). Intra-retinal fluid (IRF) was seen in 54% of second treated eyes at baseline compared with 84% in first treated eyes (p=0.01). Symptoms were absent in 54% of second treated eyes at baseline. The most common symptoms were “blurred vision” (28% of all patients) and metamorphopsia (11% of all patients). Conclusions: The visual gains achieved were not as significant as clinical trials, likely reflecting the differences in inclusion criteria of patients, and less rigorous follow-up and treatment. Intravitreal ranibizumab was effective in maintaining vision in patients with nAMD and reducing macula thickness over 5-years using an individualised treatment regime in a real-world setting.. Ranibizumab is a safe drug to use over 5-years in a real-world clinical setting. In patients undergoing treatment for nAMD in the first eye, OCT screening of the second eye at each visit may be necessary to detect second eye nAMD in this at-risk population. A large proportion of patients are asymptomatic at diagnosis of second eye disease, and a significant proportion of patients were detected to have treatable subfoveal nAMD with OCT alone. Second eye disease detected and treated by such a protocol showed a lower rate of IRF at baseline, suggesting early detection had occurred. Second eyes showed a higher rate of fluid free status at 12-months compared to the first treated eye, suggesting that early detection and treatment led to improved anatomical outcomes, potentially explaining superior VA outcomes. Patients commencing treatment in their second eye with good VA had better visual outcomes compared to those with worse VA.

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Ambrosetti, Arianna <1995&gt. "Dadamaino: dai Volumi a Sein und Zeit. Analisi dell'arte e del mercato di Eduarda Maino." Master's Degree Thesis, Università Ca' Foscari Venezia, 2020. http://hdl.handle.net/10579/16516.

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L’obiettivo principale di questa tesi è lo studio e l’analisi delle opere della pittrice milanese Eduarda Maino, in arte Dadamaino, che ha fatto del concetto di vuoto, annullamento della materia e dello spazio e dell’idea del movimento e di intangibilità il fulcro di tutto il suo percorso artistico. Nel primo capitolo verrà presentata una breve biografia dell’artista in cui verranno evidenziati gli episodi della sua vita artistica più rilevanti, le diverse relazioni intrecciate con i più grandi protagonisti del panorama culturale italiano, quali Piero Manzoni e Lucio Fontana, inoltre saranno elencate le partecipazioni di Dadamaino alle mostre nazionali ed esposizioni internazionali che hanno reso l’artista nota in tutto il mondo. Nello sviluppo dell’elaborato ci si soffermerà sui diversi cicli artistici e le numerose sperimentazioni personali: dall’esordio figurativo ed astratto informale alla scoperta del vuoto con la creazione dei suoi primi Volumi, dalle sperimentazioni optical alle installazioni ambientali e ricerche cromatiche, fino a giungere alla rivelazione segnica e dell’inconscio e la relativa produzione di un alfabeto grafico del tutto personale. Verrà inoltre prestata un’attenzione particolare anche all’archivio dell’artista, formatosi subito dopo la sua morte per il riconoscimento del suo valore artistico e lo scandalo che lo ha coinvolto nella contraffazione e autenticazione di alcune opere. Nell’ultimo capitolo si andranno infine ad analizzare gli ultimi successi ed insuccessi perseguiti dall’artista nel mercato dell’arte in riferimento alla compravendita delle opere nelle più importanti case d’asta internazionali, quali Christie’s e Sotheby’s, e nazionali, come Finarte e Il Ponte.

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Andrade, Thamyris Carvalho. "A experiência das visitas guiadas e autoguiadas : um ensaio interpretativo pelo Parque Nacional da Chapada dos Veadeiros – GO." reponame:Repositório Institucional da UnB, 2014. http://repositorio.unb.br/handle/10482/16516.

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Dissertação (mestrado)—Universidade de Brasília, Centro de Excelência em Turismo, Mestrado Profissional em Turismo, 2014. Submitted by Larissa Stefane Vieira Rodrigues (larissarodrigues@bce.unb.br) on 2014-10-15T18:58:09Z No. of bitstreams: 1 2014_ThamyrisCarvalhoAndrade.pdf: 5654280 bytes, checksum: 94a8d386372bd636ddb3abff6f2c51c9 (MD5) Approved for entry into archive by Tania Milca Carvalho Malheiros(tania@bce.unb.br) on 2014-10-16T17:50:23Z (GMT) No. of bitstreams: 1 2014_ThamyrisCarvalhoAndrade.pdf: 5654280 bytes, checksum: 94a8d386372bd636ddb3abff6f2c51c9 (MD5) Made available in DSpace on 2014-10-16T17:50:23Z (GMT). No. of bitstreams: 1 2014_ThamyrisCarvalhoAndrade.pdf: 5654280 bytes, checksum: 94a8d386372bd636ddb3abff6f2c51c9 (MD5) Os estudos sobre a experiência do turista em áreas protegidas são importantes para que se possa conhecer o processo da viagem e suas peculiaridades, tanto do ponto de vista da motivação inicial quanto dos impactos (positivos e negativos) deixados na localidade. O Parque Nacional da Chapada dos Veadeiros (PARNA-CV), criado em 1961, recebe em média 20 mil turistas por ano e, em janeiro de 2013, passou a oferecer a opção da visita sem a obrigatoriedade de contratação do guia de turismo, em atendimento à Instrução Normativa 8/2008, do Instituto Chico Mendes de Conservação e Pesquisa – ICMBIO. Tal ação suscitou manifestações de contrariedade principalmente pelo grupo de Guias locais, e tornou-se o tema/problema que motivou essa dissertação, que fez investigações com o objetivo de discutir até que ponto o turista necessita realmente de um mediador para suas visitas em áreas naturais protegidas e como visitas realizadas pelo guia de turismo ou as autoguiadas impactam diferentemente a viagem e a percepção da experiência pelo turista. O referencial teórico trata da visitação e do uso turístico de áreas protegidas, da relação do guiamento com a experiência turística da visitação. O método da fenomenologia trouxe a experiência do PARNA-CV para dialogar com a teoria e verificar, a partir da identificação de aspectos essenciais, os prós e contras das visitas guiadas e autoguiadas. Como tal realidade é produzida e pode ser interpretada, inclusive em suas dimensões conflituosas ou potenciais, é o resultado que se traz para a pesquisa. ______________________________________________________________________________ RESUMEN Los estudios sobre la experiencia del turismo en las áreas protegidas son importantes para que se pueda conocer el proceso del viaje y sus peculiaridades, tanto desde el punto de vista de la motivación como los impactos iniciales (positivos y negativos) abandonaron la localidad. El (PARNA-CV) Parque Nacional de Chapada dos Veadeiros, establecida en 1961, recibe un promedio de 20.000 turistas al año, y en enero de 2013, comenzó a ofrecer la opción de visitar sin contratación obligatoria de los guías de turismo en cumplimiento, el Instituto Chico Mendes para la Investigación y la Instrucción 8/2008 de Conservación - ICMBIO. Esta acción provocó expresiones de molestia principalmente por el grupo de guías locales, y se convirtió en el tema / problema que motivó esta disertación, que hizo investigaciones con el objetivo de discutir el grado en que los turistas realmente necesitan un mediador para sus visitas en las áreas naturales proteger y cómo las visitas de guía turístico o viaje de auto-guiado y el impacto de manera diferente a la percepción de la experiencia turística. El marco teórico es el de visitas y el turismo el uso de las áreas protegidas, la proporción guía con la experiencia turística de la visitación. El método de la fenomenología trajo la experiencia de PARNA CV a comprometerse con la teoría y verificar, a partir de la identificación de los aspectos esenciales, los pros y los contras de las visitas autoguiadas y guiadas. A medida que se produce dicha realidad y se puede interpretar, incluyendo sus dimensiones o posible conflicto, el resultado es que trae a la investigación.

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Єрмоленко, Світлана Василівна, Светлана Васильевна Ермоленко, Svitlana Vasylivna Yermolenko та Р. Івер. "Комп'ютерні технічні неологізми в німецькій мові". Thesis, Видавництво СумДУ, 2007. http://essuir.sumdu.edu.ua/handle/123456789/16516.

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Součková, Irena. "Strategická analýza." Master's thesis, Vysoká škola ekonomická v Praze, 2009. http://www.nusl.cz/ntk/nusl-16516.

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The aim of this thesis with the topic "Strategic analysis" is to elaborate the strategic analysis of the První zemědělská, a.s. Tuněchody company,i.e. to analyse the external and internal environment, to identify strengths, weaknesses, opportunities and threats and last but not least to conceive strategic recommendations. The thesis is divided into two main parts, the theoretical part and the application part.

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Vancell, Joseph. "E-learning for transformation? : a grounded theory investigation of the student and staff experience in two educational programmes at the University of Malta." Thesis, University of Hull, 2013. http://hydra.hull.ac.uk/resources/hull:16516.

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E-learning has become a mainstream feature in Higher Education. It is no longer restricted to the innovative practice of pioneer educators. But how are students and staff experiencing this change? This research used the Grounded Theory methodology. Two courses at the University of Malta were selected as case studies: one being a fully online course, the other adopting a hybrid approach. Extensive data were gathered through semi-structured interviews with students and lecturers. All the data were systematically analysed using established Grounded Theory methods, including constant comparison, coding and memoing, enabling the researcher to construct a conceptual model from the student and staff experience in e-learning. The thesis argues that e-learning, defined in this study as that learning facilitated online through network technologies, can be employed to support a range of pedagogies from knowledge-transmission or ‘banking education’ (Freire, 1970) methodologies to critical constructivist teaching and learning approaches. The latter, through the dialogic affordances of e-learning, allows students and educators to be engaged in critical discussion, the co-construction of knowledge and praxis. A theoretical model is presented which identifies key factors that contribute to effective e-learning in Higher Education. This model is original in that it shows how e-learning can be used to help a learning community achieve two interrelated Higher Educational objectives. First, through e-learning, students can gain the knowledge and skills required to function efficiently in society. Second, students can become conscious of and, possibly act against, the underlying social processes that work counter to the democratisation process.

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Ribeiro, Lucas Nogueira. "On Supervised multilinear filtering: applications to system identification and antenna beamforming." reponame:Repositório Institucional da UFC, 2016. http://www.repositorio.ufc.br/handle/riufc/16516.

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RIBEIRO, L. N. On Supervised multilinear filtering: applications to system identification and antenna beamforming. 2016. 73 f. Dissertação (Mestrado em Engenharia de Teleinformática) – Centro de Tecnologia, Universidade Federal do Ceará, Fortaleza, 2016. Submitted by Hohana Sanders (hohanasanders@hotmail.com) on 2016-04-25T19:02:04Z No. of bitstreams: 1 2016_dis_lnribeiro.pdf: 4353671 bytes, checksum: 3e2f82deacd3c88516976116873a34e5 (MD5) Approved for entry into archive by Marlene Sousa (mmarlene@ufc.br) on 2016-04-29T17:36:37Z (GMT) No. of bitstreams: 1 2016_dis_lnribeiro.pdf: 4353671 bytes, checksum: 3e2f82deacd3c88516976116873a34e5 (MD5) Made available in DSpace on 2016-04-29T17:36:37Z (GMT). No. of bitstreams: 1 2016_dis_lnribeiro.pdf: 4353671 bytes, checksum: 3e2f82deacd3c88516976116873a34e5 (MD5) Previous issue date: 2016-02-24 Linear filtering methods are well known and have been successfully applied to many engineering problems. However, they become unpractical when the parameter space is very large. The recently proposed assumption of system separability allows the development of computationally efficient alternatives to classical filtering methods in this scenario. In this work, we show that system separability calls for multilinear system representation and filtering. Based on this parallel, the proposed filtering framework consists of a multilinear extension of the classical Wiener-Hopf (WH) filter that exploits the separability property to solve the supervised multilinear filtering problem. System identification and antenna beamforming computer simulations were conducted to assess the performance of the proposed method. Our numerical results show our approach has smaller computational complexity and that it provides better estimation accuracy than the classical WH filter, which ignores the multilinear system structure. Métodos de filtragem linear estão bem estabelecidos e têm sido aplicados em diversos problemas de engenharia. Entretanto, eles tornam-se impraticáveis quando o espaço de parâmetros é grande. A recente hipótese de separabilidade de sistema permite o desenvolvimento de métodos computacionalmente eficientes neste cenário. Neste trabalho, nós mostramos que a separabilidade de um sistema leva à sua representação multilinear. Em vista disso, o método de filtragem proposto consiste em uma extensão multilinear do filtro de Wiener-Hopf (WH) clássico, que explora a separabilidade para resolver o problema de filtragem multilinear supervisionada. Simulações computacionais de identificação de sistemas e formatação de feixes de antenas foram realizadas para a avaliação do desempenho do método proposto. Nosso resultados numéricos mostram que nossa abordagem possui menor complexidade computacional e que ela fornece melhor acurácia de estimação que o filtro de WH clássico, que ignora a estrutura multilinear do sistema.

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Hjort, Amanda. "Könsroller och Härskartekniker i Twilight : (re)produktion av patriarkalgenusstrukturer genom smäktande kärlekshistoria?" Thesis, Södertörns högskola, Lärarutbildningen, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-16516.

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This essay aims to describe and problematize gender roles and master suppression techniques in Stephenie Meyer’s Twilight Saga. This is done in order to enable me, in my future profession as a teacher, to start an emancipatory discussion in class where pupils can become conscious of different ways of reading the love story. I will use the following two research questions to fulfil the purpose: 1) investigate which gender roles that appears in the book’s main characters Bella and Edward and 2) which master suppression techniques that colours their relationship. To answer the questions gender theory and ideology-critics are used. Gender theory is first and foremost used to analyse gender roles whereas ideology-critics is a method of reading that highlights the importance of taking the society and thereto connected values in to account. Applying these two theories on the book it becomes clear that the main characters follows traditional gender roles for what is seen as typical feminine and masculine behaviour; Bella is caring, passive, sexually loyal, and addicted and Edward is aggressive, physically strong and fast, stubborn, dominant and protective. Further more, it is also evident that these roles are accompanied by a number of master suppression techniques used by Edward, such as: make Bella invisible and silly, keep information from her, and use of violence and threats. By using the knowledge in a pedagogical fashion pupils can be energized to start critically reflecting about these stereotypical roles and thereby emancipate from them. They will realize that Stephenie Meyer’s Twilight Saga is a re-production of patriarchal gender structure through an emotional love story.

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Strandberg, Åsa. "-Jag älskar att vara kvinna! Eller, vad är det för kvinnligt med Kvinnobiblioteket?" Thesis, Högskolan i Borås, Institutionen Biblioteks- och informationsvetenskap / Bibliotekshögskolan, 1995. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-16516.

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"What's feminine about the Women's library?" This library, Kvinnobiblioteket, is abranchoffice at Björkskatan in the city of Luleå, and the introducing question is thestartingpoint of this essay. To find some possible answers is its object.One way of describing what's feminine about Kvinnobiblioteket is through presenting itsobjective, ambitions and methods of working. That is done mostly in a chapter headlined"Kvinnobiblioteket", and shows how the women's perspective concerns for exarnplepurchase of books and magazines, displaying of books, the opening hours, design etc.Although the focus in the survey is not in the former kind of description, but in the interviewsbeing made during a two week long period of time with ten women at Kvinnobiblioteket(both library user and personnel) at spring 1995. The results of the interviewsthen have been compared with theories from psychological-, feminist-, literature- andlibrary and information sciences, as we11 as to former surveys and essays.The material together leads to some keywords that seem to be important when trying tofind out what's female about the women's library. They are, for example, such as:accepting, care, solidarity, sensitive ears and keenly awareness of the customers needs,commitment and willingness to cooperate. The meaning of the keywords are somewhatexpressed in the objective of the women's library, to stress female knowledge andexperience, to make them visible.

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Barros, Nelma Maria Felix Capi Villaça de Souza. "Avaliação e ensino do repertório de leitura em indivíduos com Síndrome de Down com o uso do software educacional Mestre." Pontifícia Universidade Católica de São Paulo, 2009. https://tede2.pucsp.br/handle/handle/16516.

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Made available in DSpace on 2016-04-28T20:57:54Z (GMT). No. of bitstreams: 1 Nelma Maria Felix Capi Villaca de Souza Barros.pdf: 11216905 bytes, checksum: 8e2496321e49f65e67405bbde825da02 (MD5) Previous issue date: 2009-05-06 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior The increasing number of students with special educational needs in regular education demands for new methodologies which can help the teacher in his professional activities. Reading is the basis for acquiring further academic repertoires, as well as for the individual s participation in society. Unfortunately difficulty in reading has been identified in different grades of the academic period of Brazilian s students. Reading has been an object of study in different approaches, including the Behavior Analysis, which presents studies based on the stimulus-equivalence paradigm, with good results in teaching basic reading repertoires (words and phrases). A part of the studies was carried out using the Mestre® software; however, there are very few studies in which the participants are only individuals with Down s Syndrome. The objectives of this study are evaluating the reading repertoire of individuals with Down s Syndrome and implementing a program to teach reading using the Mestre® educational software. The participants were four students with Down s Syndrome, with ages between nine and twenty-five. The study was developed in three stages: 1) Evaluation of the participants previous reading repertoire. 2) Implementation of a teaching procedure with 15 words composed by simple syllables, focusing on the relations between stimulus of different modalities: spoken word (A), picture (B), printed word (C). The CC, AB, AC, CE and AE relations were taught and the emergence of the BC, CB, CD and BE relations was tested. 3) Application of Generalized Reading Test with news words and phrases composed by simple syllables. The repertoire of four participants was evaluated and three of them were submitted to teaching procedure. It was verified that two participants showed satisfactory performances in the trained relations as well as in the tested relations, reaching 100% correct results in expressive reading (CD relation); on the other hand, the levels in the word and phrase in the Generalized Reading Test were low, leading to assume that they were not under the control of minimal units (syllables). The participant that did not complete the teaching procedure showed positive performance changes, compared to the previously evaluated repertoire. Taking into account the short training period (a maximum of 13 hours and 35 minutes) and the number of sessions (a maximum of 21 sessions) and also the extremely small initial reading repertoire, the conclusions are that it is possible to teach reading based on the stimulus equivalence and that the Mestre® software is a valuable tool to improve reading to students with Down s Syndrome Diante do crescente número de inclusões na rede regular de ensino em nosso país, fazem-se necessárias pesquisas em novas metodologias que auxiliem o professor no processo de ensino de pessoas com necessidades educacionais especiais, principalmente na leitura, que é base para a aquisição dos demais repertórios acadêmicos e atuação do indivíduo na sociedade. É fato também que a dificuldade na aprendizagem de leitura tem sido identificada em diferentes anos no período acadêmico. A leitura tem sido objeto de estudo em diferentes abordagens, entre elas a Análise do Comportamento, a qual tem estudos pautados no paradigma da equivalência de estímulos, com resultados promissores para a aquisição e aperfeiçoamento de repertórios básicos de leitura, envolvendo o ensino de palavras e de um segmento mais amplo como frases. Parte dos estudos foi realizada com o software Mestre®, no entanto são escassos os estudos tendo como participantes apenas indivíduos com Síndrome de Down. O presente estudo teve por objetivo, avaliar o repertório de leitura de indivíduos com Síndrome de Down e implementar uma proposta de ensino de leitura, utilizando o software educativo Mestre®. Participaram da pesquisa quatro indivíduos, tendo entre nove e vinte e cinco anos, todos com diagnóstico de Síndrome de Down. O estudo foi desenvolvido em três etapas: 1) Avaliação do repertório prévio dos participantes em leitura. 2) Implementação de um procedimento para o ensino de 15 palavras compostas por sílabas simples, focalizando as relações entre estímulos de diferentes modalidades: palavra falada (A), figura (B), palavra impressa (C), sendo ensinadas as relações: CC, AB, AC, CE e AE e testada a emergência das relações BC, CB, CD e BE. 3) Teste de Leitura Generalizada de palavras e frases formadas por sílabas simples. Dos quatro participantes, três passaram pelo procedimento de ensino. Verificou-se que os dois participantes que concluíram o procedimento obtiveram desempenhos satisfatórios tanto nas relações treinadas, quanto nas relações testadas, atingindo 100% de acertos na leitura expressiva (relação CD); porém nos testes de generalização de leitura de palavras e frases os patamares foram baixos, levando a pressupor que eles não ficaram sob controle de unidades mínimas (sílabas). O participante que não concluiu o procedimento de ensino teve alterações positivas no desempenho, comparativamente ao repertório prévio avaliado. Considerando o pequeno tempo de treino (máximo de 13 horas e 35 minutos) e de sessões (máximo de 21 sessões) a que os participantes se submeteram durante o procedimento de ensino e os desempenhos apresentados e levando em conta o baixíssimo repertório inicial de leitura, conclui-se que é possível pautar-se na equivalência de estímulos para o ensino de leitura e que a utilização do software Mestre® mostrou-se uma ferramenta valiosa para o ensino e aperfeiçoamento da leitura em pessoas com Síndrome de Down

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Nordqvist, Filip, and Erik Sahlbom. "Visual Narrative Game Design : Ett narrativ utan konversation." Thesis, Blekinge Tekniska Högskola, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-16516.

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Vi undersöker den narrativa stilen som Flower och Journey använder sig av. I denna narrativastil finns det varken dialog och text. Utifrån Flower och Journey skapade vi en designmetodsom ska finnas till som inspiration för utvecklare som vill testa denna stilen. Resultatet avundersökningen ger upphov till en designmetod som vi applicerar på vår gestaltning för atttesta den i en annan spel genre. Mycket av metoden är fokuserad på hur Flower och Journeygör då vi bara undersöker de två spelen. Vi skulle vilja göra en mer generell undersökning dåvi undersöker mer spel som också har ett narrativ utan dialog och text. We study the narrative style that Flower and Journey uses. In this narrative style, there is nodialogue and text. Based on Flower and Journey, we created a design method that will be aninspiration for developers who want to test this style. The result of this bachelor thesis givesrise to a design method that we apply to our game idea this applies the method to anothergame genre. Much of the method is very focused on what Flower and Journey do when weonly examine these two games. We would like to do a more general study when we examinemore games that also have a narrative without dialogue and text.

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Liang, Shu-Yuan. "Opioid-taking self-efficacy in Taiwanese Outpatients with cancer pain." Thesis, Queensland University of Technology, 2007. https://eprints.qut.edu.au/16516/1/Shu-Yuan_Liang_Thesis.pdf.

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Despite the fact that as many as 80-90% of patients with cancer pain can be effectively treated using pharmacological therapies and other advanced approaches, 31% to 85% of cancer patients in Taiwan still experience varying levels of pain. Pain is one of the symptoms that patients fear most; it overwhelms all aspects of patients' lives and creates a sense of uncertainly and hopelessness. Pain control is, therefore, a high priority in the treatment of cancer patients. Pharmacological therapy is the cornerstone of cancer pain management. With the current trend toward outpatient care, many patients are being required to assume greater responsibility for self-management of prescribed analgesics at home to deal with the variable and complex nature of cancer pain and side effects of opioids. Patients however, have misconceptions regarding analgesics and a series of difficulties when attempting to put a pain management regimen into practice. This research addressed the hypothesis that self-efficacy beliefs might play an important role in analgesic adherence and pain experience in Taiwanese outpatients with cancer. The purpose of this study was to develop a scale to measure the self-efficacy expectations relating to opioid-taking in Taiwanese outpatients with cancer. Another purpose was to explore how opioid-taking self-efficacy and beliefs about opioid analgesics contribute to patients' analgesic adherence and pain experience in Taiwanese outpatients with cancer. In the first stage semi-structured interviews were conducted to collect data from a purposeful sample (n=10) of oncology outpatients from two teaching hospitals in the Taipei area of Taiwan. The purpose of this phase was to identify behaviours and situational impediments associated with analgesic taking. Findings from this phase were used to develop a scale to measure opioid-taking self-efficacy. In the second stage a pilot test with a convenience sample (n=30) was conducted to test the validity and reliability of the new scale and to identify the feasibility of using the scale in a cross-sectional survey. In the third stage a cross-sectional survey was undertaken (n=92) to describe pain experiences, analgesic adherence, beliefs about opioid analgesics, and opioid-taking self-efficacy in Taiwanese outpatients with cancer and to explore how opioid-taking self-efficacy and beliefs about opioid analgesics contributed to analgesic adherence and pain experience. Results of this study highlight an important issue - under-treatment of cancer pain in this group of Taiwanese outpatients. As well, low adherence rates to opioid analgesics in cancer outpatients arose as an important issue in this study. A range of misconceptions about using opioids for pain was also common amongst the sample. Despite these misconceptions, patients reported being moderately confident in their ability to perform self-management behaviours related to their prescribed opioid-taking. Results of this research supported the notion that patients' self-efficacy in relation to taking their prescribed opioid regimen was a significant independent predictor of patients' adherence behaviour and pain relief, but not of pain severity. Beliefs about opioid analgesics were also an independent predictor of patients' adherence, but not of pain relief or pain severity. In addition, findings from this study provided support for the validity and reliability of the opioid-taking self-efficacy scale. Results suggested there is a need for systematic assessment of beliefs affecting patients' opioid adherence behaviours for cancer pain control, including perceived personal self-efficacy and beliefs about opioid analgesics. Educational programs that focus on overcoming patients' misconceptions (beliefs) about taking opioid analgesics may be particularly beneficial. In addition, this study advocates that conducting self-efficacy-enhancing interventions may improve medication adherence for patients and therefore pain relief. More research is needed to demonstrate the construct validity of the self-efficacy scale and to evaluate self-efficacy enhancing interventions in cancer pain management.

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Silva, Eudes Henrique da. "Reflex?es filos?ficas em torno da constitui??o do sujeito religioso-moral no pensamento de Freud." Universidade Federal do Rio Grande do Norte, 2013. http://repositorio.ufrn.br:8080/jspui/handle/123456789/16516.

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Made available in DSpace on 2014-12-17T15:12:18Z (GMT). No. of bitstreams: 1 EudesHS_DISSERT.pdf: 1089303 bytes, checksum: 4103203dba0cb438a4c808f71fa235b6 (MD5) Previous issue date: 2013-09-27 Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior The present research deals with a philosophical reflection about the constitution of the subject religious and moral in the thought of Freud, starting from of question of religion while one of the various spaces concretion of the individual morality. Our hypothesis is that religion presents itself as a space of revival of the primary relationship with the mother of the subject and as a moral agency. That primary relationship corresponds to the period before the Oedipus complex. The cut caused in the Oedipus complex sake in the an emptiness the subject, leading him to a situation of helplessness. In trying to fill the emptiness and consequently out of the situation of displeasure occasioned by the helplessness, the individual seeks diverses means, between which, the religion. The religion, that sense, quest for one part, that support be filling of the existential emptiness, triggered in the Oedipus complex, and on the other, works as a staunch ally of the Superego, which for turn is direct heir of the Oedipus complex and whose function is to require of the subject to moral living, as is established by the social body, where the individual is inserted. Therefore, we seek to draw this subject starting from general ideas of the philosophy, about the moral, as well as some theoretical elements of freudian thought, since his idea of the origin of the culture, morality and religion the more specific elements that pertain to the individual subject, ie, the psychism A presente pesquisa trata de uma reflex?o filos?fica em torno da constitui??o do sujeito religioso-moral no pensamento de Freud, partindo da quest?o da religi?o, enquanto um dos v?rios espa?os de concre??o do indiv?duo moral. Partimos da hip?tese de que a religi?o se apresenta como espa?o de revivesc?ncia da rela??o prim?ria do sujeito com a m?e e como uma ag?ncia moral. Essa rela??o prim?ria corresponde ao per?odo que antecede o complexo de ?dipo. O corte provocado no complexo edipiano causa no sujeito um vazio, levando-o a uma situa??o de desamparo. Na tentativa de preencher o vazio e consequentemente sair da situa??o de desprazer, ocasionado pelo desamparo, o indiv?duo procura meios diversos, entre os quais, a religi?o. A religi?o, nesse sentido, busca por um lado, ser esse suporte de preenchimento do vazio existencial, provocado no complexo de ?dipo e, por outro, funciona como uma fiel aliada do Superego que, por seu turno, ? herdeiro direto do complexo edipiano e cuja fun??o ? exigir do sujeito a viv?ncia moral, conforme se ? estabelecido pelo corpo social, no qual o indiv?duo est? inserido. Assim sendo, buscamos desenhar esse sujeito a partir de ideias gerais da filosofia acerca da moral, bem como de alguns elementos te?ricos do pensamento freudiano, desde sua ideia de origem da cultura, moralidade e religi?o a elementos mais espec?ficos que dizem respeito ao sujeito individual, ou seja, ao psiquismo

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Doré, Cécile Hoornaert Alain. "Odontologie et instruments de musique à vent." [S.l.] : [s.n.], 2007. http://castore.univ-nantes.fr/castore/GetOAIRef?idDoc=16516.

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PAIVA, GUILHERME FRANCA DOS SANTOS. "FUNCTIONAL LITERACY AND FOOD NUTRITION LABELING." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2010. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=16516@1.

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COORDENAÇÃO DE APERFEIÇOAMENTO DO PESSOAL DE ENSINO SUPERIOR O presente estudo tem como objetivo avaliar o nível de entendimento dos consumidores aplicado à rotulagem nutricional de alimentos industrializados. Ele suporta a idéia de que cada consumidor funciona de maneira distinta frente ao contexto de análise dos rótulos nutricionais. Através de uma pesquisa empírica que utiliza a metodologia de um sistema de avaliação de letramento funcional, traça-se o perfil dos consumidores e mensura-se a pontuação dos entrevistados frente ao conhecimento de aspectos da rotulagem nutricional aplicada pela Agência Nacional de Vigilância Sanitária. Os resultados mostram evidências de que existem consumidores desconhecedores de elementos nutricionais básicos, outros incapazes de desenvolver tarefas simples de análise de rótulos. Níveis diferentes de entendimento frente as variáveis sexo, renda, escolaridade, idade, leitura de rótulos, exercícios, prática de refeições fora de casa e compra de alimentos para a família revelam realidades heterogêneas de escolhas alimentares, trazendo subsídios importantes para a área de comportamento do consumidor e de políticas públicas. This study aims to evaluate the level of understanding of consumers in the context of food nutrition labeling. It supports the idea that each consumer works differently against the background of the analysis of nutrition labels. Through an empirical research that uses the methodology of an assessment system for functional literacy, a profile of consumers is drawn and a score of the respondents related to nutrition label aspects knowledge applied by Anvisa is measured. Results show evidence that consumers are unaware of basic nutritional elements, unable to develop other simple tasks of label analysis. Different levels of understanding by the variables gender, level of income, level of education, age, reading labels habit, exercise habit, practice of eating out and buying food for the family reveal heterogeneous realities of food choices, bringing important benefits to the area of consumer behavior and public policy.

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Knorn, Jan. "Studying land-use and land-cover change with high resolution data." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2012. http://dx.doi.org/10.18452/16513.

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Naturschutzgebiete sind ein essentieller Bestandteil zur Wahrung natürlicher Lebensräume. Oft verfehlt die Einrichtung solcher Schutzzonen jedoch den erwarteten Effekt. Die größte Gefahr liegt hierbei neben dem Klimawandel im direkten Einfluss des Menschen. Besonders in Phasen sozioökonomischen Umschwungs und damit verbundenen Landnutzungsveränderungen oder auch illegaler Nutzung natürlicher Ressourcen, sind Naturschutzgebiete in ihrer Funktion gefährdet. Ziel dieser Arbeit ist somit, Ursachen des Landnutzungswandels sowie dessen Auswirkungen und Ausmaß am Beispiel des rumänischen Teils der Karpaten-Ökoregion abzuleiten. Das Untersuchungsgebiet ist ein wichtiges Zentrum für Biodiversität und in ihm befindet sich Osteuropas größte gemäßigte Waldregion sowie einige der letzten europäischen Urwälder. Rumänien umschließt mehr als die Hälfte der Karpaten und es ist hiernach von besonderem Interesse, Gründe und Auswirkungen des rezenten post-sozialistischen Landschaftswandels zu untersuchen. Mit Hilfe von Landsat Aufnahmen sowie einer ad hoc entwickelten Methode zur Klassifizierung großräumiger Gebiete, wurden Veränderungen in der Waldbedeckung für die post-sozialistische Zeit abgeleitet. Die Ergebnisse offenbaren großflächige Forstveränderungen, auch innerhalb von Naturschutzgebieten und Urwäldern. Institutionelle Umbrüche und eine rapide Umgestaltung in den Eigentumsverhältnissen, wurden als Hauptursachen herausgestellt. Rumänische Naturschutzgebiete erreichen nicht die gewünschte Effektivität und Urwäldern werden weiterhin dezimiert. Die Arbeit verdeutlicht den Einfluss sozioökonomischer Veränderungen auf die Entstehung von Raubbau und legt Defizite in der Effektivität von Schutzbemühungen zum Erhalt der Biodiversität und verbundener Ökosystemleistungen offen. Protected areas are one cornerstone of conservation efforts to safeguard natural habitats from destruction and overexploitation. Still, many of these areas remain less effective than initially envisioned. Besides climate change, main threats originate from enduring human activities. Protected areas are particularly at risk during periods of rapid socio-economic changes, which can trigger widespread land-use change and illegal resource use. The main goal of this thesis is to assess the extend and underlying causes of land-use change in protected areas and forest habitats within the Carpathian Ecoregion. The Romanian Carpathians were selected as a focus area in this study, because they comprise Eastern Europe’s largest continuous temperate forest region as well as some of the last and largest tracts of European old-growth forests, and they are a major hotspot of biodiversity. Romania comprises more than half of the Carpathian Ecoregion and it is of particular interest to study the causes and effects of land-use changes, which have emerged after the collapse of socialism in 1989. Post socialist forest cover change was quantified for the last 25 years using Landsat images and an ad hoc developed large area classification technique. Results show widespread forest disturbances, even inside protected areas and old-growth forests. Drivers of these disturbances can be related to institutional change and changes in ownership. The effectiveness of Romania’s protected area network in terms of its ability to safeguard biodiversity is most likely decreasing, and intact old-growth forests continue to disappear. This thesis reveals how rapid socio-economic changes may lead to overexploitation, and highlights substantial shortcomings in the effectiveness of protection efforts to safeguard biodiversity and related ecosystem services.

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Beceren-Braun, Figen. "Molekulare Mechanismen und strukturelle Dynamik der Interaktion des leukozytären Adhäsionsrezeptors L-Selektin mit intrazellulären Liganden." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16510.

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Die Wanderung von Leukozyten aus dem Gefäßlumen läuft in einer Kaskade ab, die durch verschiedene Adhäsionsmoleküle vermittelt wird. Dabei wird der erste Kontakt der Leukozyten mit dem Endothel durch die Interaktion von LSelektin mit seinen endothelialen Sialomucinliganden eingeleitet. Neben seiner Adhäsionsfunktion kann LSelektin intrazelluläre Signalwege aktivieren und wird, induziert durch intrazelluläre Signale, an seinen cytoplasmatischen Serinresten durch Proteinkinase C (PKC) phosphoryliert. Über die Regulation dieser Serinphosphorylierung von L-Selektin ist nur wenig bekannt. Ziel dieser Arbeit war die Untersuchung der cytoplasmatischen Interaktion von L-Selektin mit dem Proteinphosphatase 2A-Inhibitor PhapII und die Identifizierung noch unbekannter Interaktionspartner der cytoplasmatischen Domäne von LSelektin. Es konnte gezeigt werden, dass das basische Duplett Lys-365/Arg-366 der cytoplasmatischen Domäne von L-Selektin essentiell für die Bindung von PhapII ist, die über den sauren CTerminus von PhapII vermittelt wird. In einem Malachitgrün-basierten Phosphataseassay konnte gezeigt werden, dass die cytoplasmatischen Serinreste von LSelektin durch Protein Phosphatase 2A (PP2A) dephosphoryliert werden. Ferner konnte in Jurkat-Zelllysaten PP2A als ein direkter Interaktionspartner von nicht phosphoryliertem LScyto identifiziert werden. Die Dephosphorylierung von LScyto konnte durch PhapII verhindert werden, was auf eine Regulation der Serinphosphorylierung durch PKC, PP2A und PhapII hindeutet. Mit dem Ziel, die Interaktion von PhapII mit L-Selektin durch Co-Kristallisation zu untersuchen, wurde PhapII rekombinant exprimiert und gereinigt. Mittels chromatographischer Verfahren konnte PhapII als ein Protein mit intrinsischer Unordnung in seiner Sekundärstruktur beschrieben werden. Die Deletion des sauren C-Terminus bzw. die Mutation an Serin-9 von PhapII zeigten einen Einfluss auf die Konformation von PhapII zu einer kompakteren Proteinstruktur hin. Migration of leukocytes from the lumen occurs in a cascade mediated by different members of adhesion molecules. The first contact of leukocytes to endothelium is initiated by the interaction of Lselectin to its endothelial sialomucin ligands. Besides its role in cell adhesion, Lselectin can induce a set of intracellular signaling pathways and can be a target of serine phosphorylation by protein kinase C (PKC) induced by intracellular signals. But little information on regulation of this serine phosphorylation of L-selectin and therefore regulation of intracellular protein interactions is known. The aim of this thesis was to analyze the cytoplasmic interaction of L-selectin with the inhibitor of protein phosphatise 2A PhapII and searching for other unknown interaction partners of the cytoplasmic domain of L-selectin. Results show that the basic doublet Lys-365/Arg-366 of the cytoplasmic domain of Lselectin (Lscyto) is essential for binding to PhapII which is mediated by the acidic Cterminal tail of PhapII. Using malachite green based assay to detect free phosphate, cytoplasmic serine residues of Lselectin were dephosphorylated by protein phosphatase 2A (PP2A). In addition, it was possible to identify PP2A as a direct interaction partner of the non-phosphorylated domain of Lselectin in Jurkat T cell lysates. The dephosphorylation of Lscyto was prevented by PhapII and points the regulation of serine phosphorylation by PKC, PP2A and PhapII. For analysis of the interaction of PhapII with L-selectin by co-crystallography, PhapII was recombinantly expressed and purified. Using chromatographic techniques PhapII was observed to be a protein with an intrinsic disorder in its secondary structure. Deletion of the acidic C-terminus and in particular mutation of Serine-9 showed an effect on the conformation of PhapII to a globular and compact protein structure.

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Bender, Jennifer. "Biochemische und funktionelle Charakterisierung der zell-assoziierten Phospholipase A, PlaB, von Legionella pneumophila." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16116.

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L. pneumophila, der Erreger der Legionärskrankheit, kodiert für eine Vielzahl lipolytischer Enzyme. Bis zu 17 verschiedenen Proteinen kann aufgrund von Sequenzhomologien oder experimenteller Analyse phospholipolytische Eigenschaft zugeschrieben werden. Neben sekretierten Formen wird eine besonders aktive zell-assoziierte Variante exprimiert, die Phospholipase A/Lysophospholipase A PlaB. Wie bereits gezeigt werden konnte, kodiert das plaB Gen für die hauptsächliche membranständige Phospholipase A von L. pneumophila mit Enzymaktivitäten, die die Aktivität sekretierter Proteine um das 100-fache übersteigen. Da PlaB zu keiner der bisher beschriebenen Phospholipasen Homologien aufweist, wurden in dieser Arbeit durch gezielte Mutagenese die katalytisch wichtigen Aminosäuren identifiziert. Dies ergab, dass PlaB zwar eine für Lipasen und Proteasen typische katalytische Triade aus Serin, Asparat und Histidin ausbildet, die umliegenden Motive sich aber deutlich von bisher beschriebenen Enzymklassen unterscheiden. Somit stellt PlaB das erste näher charakterisierte Mitglied einer neuen Familie phospholipolytischer Enzyme dar. Im Weiteren konnten für die Substratspezifität wichtige Aminosäurereste identifiziert werden. Dabei stellte sich heraus, dass die Fähigkeit zur Hydrolyse von cholinkettentragenden Substraten besonders suszeptibel gegenüber Mutationen war. Da im Vergleich zu nicht-pneumophila Stämmen, wie z. B. L. spiritensis, nur L. pneumophila in der Lage war, diese Lipide in hohem Maße umzusetzen, kann die Eigenschaft von PlaB, Phosphatidylcholin (PC) zu hydrolysieren, einen Virulenzvorteil für L. pneumophila bedeuten. Die Hypothese konnte durch Hämolyse-experimente bestärkt werden. Hier zeigten sich Mutanten mit reduziertem Potential zur Hydrolyse von PC weniger zytotoxisch gegenüber humanen Erythrozyten. Das zell-zerstörende Potential von PlaB könnte somit eine enorme Auswirkung auf die Virulenzeigenschaften von L. pneumophila haben. Wie in der vorliegenden Arbeit untersucht, bestätigten in vitro Experimente, dass PlaB die hauptsächliche Aktivität während einer Makrophageninfektion darstellt, die Deletion des Gens aber keine Auswirkungen auf das Replikationspotential der Bakterien hat. Ganz im Gegenteil dazu waren plaB Insertionsmutanten bei der Infektion von Meerschweinchen in ihrer Vermehrungsfähigkeit in der Lunge als auch in der Verbreitung der Erreger zur Milz der Tiere reduziert. Um den Grund des Defektes näher zu erörtern, wurde in einem Screen auf 40 verschiedene Entzündungsmediatoren die Sekretion von IL-8, MCP-1, RANTES und TIMP-2 als PlaB-abhängig identifiziert. Somit repräsentiert die zell-assoziierte Phospholipase A, PlaB, von L. pneumophila eine neue Klasse lipolytischer Enzyme und kann durch Hydrolyse eines breiten Substratspektrums, insbesondere durch Hydrolyse von PC, die Vermehrung und Verbreitung des Erregers im Wirtsorganismus unterstützen. L. pneumophila, the causative agent of Legionnaires’ disease (LD) expresses numerous lipolytic enzymes. According to sequence homology or determined lipolytic activities, up to 17 open reading frames of the L. pneumophila genome may encode functional phospholipases. In addition to secreted and/or injected lipolytic enzymes, it was shown that the pathogen expresses a highly active and membrane-bound phospholipase A/lysophospholipase A with hemolytic activity, designated PlaB. As PlaB does not belong to any established bacterial or eukaryotic protein family of lipolytic enzymes nor does it show sequence homology to conserved motifs harboring the catalytically important amino acids, we analyzed putative catalytic centers using site-directed mutagenesis. This study shows that PlaB exhibits a catalytic triad of serine, aspartate and histidine residues, most commonly found within lipolytic and proteolytic enzyme families. However, surrounding motifs differ significantly from described ones. Thus, PlaB is the first representative of a new class of lipolytic enzymes. In addition, we described amino acids important for substrate specificity, revealing that the ability to hydrolyze phosphatidylcholine (PC) is severely susceptible to various mutations. Since PlaB of non-pneumophila strains, such as L. spiritensis, express comparable activities against glycerol-containing lipids, but are reduced in their hydrolytic potential to cleave choline-containing substrates, PC-targeting activity could be an important contribution to the pathogenicity of L. pneumophila, the most common cause of LD. The hypothesis was underlined by reduced hemolytic potential of L. spiritensis PlaB and PC-hydrolysis impaired mutants of L. pneumophila PlaB and is in accordance with PC being the major lipid in the outer leaflet of eukaryotic membranes. The cell destructive properties of PlaB may enhance bacterial pathogenicity in multiple ways. As depicted within this study, PlaB represents the major lipolytic activity present throughout host cell infections; however, gene deletion mutants retained their ability to multiply within several host cell infection systems. On the contrary, the plaB mutant strain was inhibited in replicating in the lung and disseminating to other organs in a guinea pig infection model. To elucidate the impact of PlaB on Legionella virulence we investigated 40 inflammatory factors secreted by lung epithelial cells upon Legionella infection and observed that IL-8, MCP-1, RANTES and TIMP-2 are released in a PlaB-dependent manner. Thus, PlaB represents a new family of lipolytic enzymes which could, according to the lipolytic profile and especially the ability to hydrolyse PC, contribute to replication and dissemination properties of a pathogen within a host cell, e.g. amoeba, or even more complex organisms such as guinea pigs or humans.

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Hoffmann, Frauke. "Mating behavior as non-invasive biomarker in Xenopus laevis for the assessment of endocrine disrupting compounds." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16519.

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Hormonell wirksame Chemikalien, wie Pflanzenschutzmittel oder Pharmaka gelangen durch Abwässer in die Umwelt und akkumulieren vor allem in Oberflächengewässern. Ein erhöhtes Augenmerk liegt auf Substanzen, die durch (anti)androgene und (anti)östrogene Wirkungsweise die Reproduktion von Tieren und Menschen beeinträchtigen. Bei den bisherigen Nachweismethoden für diese Stoffe handelt es sich um invasive Methoden, die das Töten der Tiere beinhalten. Diesen Methoden mangelt es jedoch an der nötigen Sensitivität, um umweltrelevante Konzentrationen der endokrinen Disruptoren (EDs) nach Kurzzeitexposition nachweisen zu können, sowie am Vermögen, alle vier Wirkmechanismen (androgen, antiandrogen, östrogen und antiöstrogen) mit einer einzelnen Testmethode feststellen und unterscheiden zu können. In dieser Studie wurde deshalb mit Hilfe männlicher Afrikanischer Krallenfrösche (Xenopus laevis) eine Testmethode entwickelt, bei der die Frösche verschiedenen (anti)androgenen und (anti)östrogenen EDs ausgesetzt wurden und ihr Rufverhalten untersucht wurde. Diese nicht-invasive Methode erwies sich als schnell und höchst sensitiv. Zudem war es erstmals möglich, die vier verschiedenen Wirkmechanismen allein anhand veränderter Ruftypen und Rufparameter zu bestimmen und zu unterscheiden. Darüber hinaus konnte gezeigt werden, dass bei Anwendung dieser Methode die Möglichkeit besteht, die Versuchstiere in weiteren Tests wiederzuverwenden, da die Rufparameter nach einer expositionsfreien Zeit von sechs Wochen wieder Kontrollwerte erreichten. Zusammengefasst kann die hier vorgestellte verhaltensphysiologische und damit nicht-invasive Methode als Biomarker für den Nachweis von (anti)androgenen und (anti)östrogenen EDs verwendet werden. Ferner zeigt die hohe Sensitivität des Tests, sowie die Möglichkeit der vollautomatischen Analyse enormer Datenmengen, dass dieser schnelle Verhaltenstest ein großes Potential hat, ein sensitiver, standardisierter und nicht-invasiver Biomarker zu werden. Endocrine disrupting compounds (EDCs), such as herbicides, pesticides or pharmaceuticals enter the environment via sewage effluents and especially accumulate in surface waters. Research efforts so far mainly focused on EDCs with (anti)androgenic and (anti)estrogenic modes of action (MOAs), which can interfere with reproductive biology of vertebrates. To date, biomarkers for the assessment of such compounds are invasive techniques, which are not sensitive enough to detect EDCs after short-term exposures and which cannot distinguish between the four MOAs. Hence, in this study a non-invasive method for the assessment of EDCs was developed using male African clawed frogs (Xenopus laevis) as model species. Frogs were exposed to individual (anti)androgenic and (anti)estrogenic EDCs in the surrounding water and their calling behavior was analyzed. This non-invasive method turned out to be a fast and highly sensitive biomarker for the detection of (anti)androgenic and (anti)estrogenic EDCs. Moreover, this method was able to differentiate between the four different MOAs solely by determining affected parameters of the calling behavior. It was also shown that by using this method, it might be possible to reuse already tested experimental animals, because the measured affected parameters were reversed after a period of six weeks under control conditions. Taken together the here established non-invasive behavioral method can be used as biomarker for the detection of (anti)androgenic and (anti)estrogenic EDCs. Furthermore, the high sensitivity of this testing method, as well as the possibility of analyzing vast datasets rapidly in a completely automated fashion indicate the huge potential for this rapid behavior test to become a sensitive, standardized, non-invasive biomarker.

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Weinreich, André. "Projektiv-Evaluatives Priming." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2011. http://dx.doi.org/10.18452/16416.

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Eine Vielzahl von Studien zeigt, dass die Valenz eines Reizes nicht nur das Entscheidungs- und Urteilsverhalten gegenüber diesem, sondern auch gegenüber nachfolgenden Reizen beeinflusst. Jedoch ist nicht ausreichend untersucht, wie eine solche kontextuelle emotionale Stimulation Einfluss auf nachfolgende Urteile und Entscheidungen einer Person nimmt. Repräsentativ für eine Vielzahl ähnlicher Prozeduren habe ich in der vorliegenden Arbeit jene funktionellen Mechanismen untersucht, die dem Einfluss der Valenz einer Stimulation auf nachfolgende bewertende Urteile unterliegen. Dabei habe ich mich auf das Paradigma des Projektiv Evaluativen Priming (PEP) konzentriert, in welchem die zu beurteilenden Reize emotional neutral sind. Die Ergebnisse des ersten Teils der Arbeit legen nahe, dass Priming durch supraliminal präsentierte Primes unabhängig von der Prime-Klasse (Wörter, Bilder von Umweltszenen, Bilder von Gesichtsausdrücken), zuverlässig auftritt. PEP ist deshalb nicht durch die Annahmen der Feeling As Information Hypothese erklärbar. Die Ergebnisse des zweiten empirischen Blocks zeigen außerdem, dass PEP besser durch eine im Rahmen der Dissertation entwickelte Theorie des Cognitive Emotional Compound (CEC) als durch das assoziativ-semantische Netzwerkmodell beschreib- und vorhersagbar ist. Im dritten Teil der Arbeit konnte ich beobachten, dass der Einfluss eines subliminal präsentierten Prime auf nachfolgendes bewertendes Urteilsverhalten mit dem zeitlichen Abstand zwischen Prime und Target (SOA), und darüber hinaus mit der Interozeptiven Bewusstheit der Person steigt. Diese Ergebnisse legen nahe, dass das Resultat der impliziten emotionalen Verarbeitung eines Reizes körperlich repräsentiert ist, und dass ein bewertendes Urteil über einen Reiz bzw. Compound, auf der subjektiven Wahrnehmung solcher valenzindikativen, körperlich verankerten Signale beruht. Judgment and decision are biased by contextual emotional stimulation. However, it has not yet been sufficiently examined how this influence is mediated. Therefore, in this work I investigated the functional mechanisms that underlie the impact of the valence of a prime stimulus on subsequent evaluative judgments. Specifically, I focused on the paradigm of Projective Evaluative Priming (PEP), where the target stimuli that have to be explicitly evaluated are emotionally neutral. The results of the first part of the current work indicate that priming with supraliminally presented primes occurs reliably across diverse classes of primes (words, landscapes, pictures of facial expressions). These findings are incompatible with a core assumption of one of the most prominent theories about affective influence on judgment and decision, the Feeling As Information hypothesis (Schwarz & Clore, 1983, 2003). The findings of the second part show that supraliminal priming increases with the prime’s cognitive complexity, emotional intensity, and the participants’ cognitive resources during target processing. In contrast, priming decreases with the extent to which the participants allocate cognitive resources during prime processing. These findings indicate that PEP is better explained and predicted by the theory of the cognitive-emotional compound (CEC) than the prominent associative semantic network model (e.g. Bower, 1981). The CEC has been developed by the author within the current dissertation process. In the third part of the current work I observed that the impact of a subliminally presented prime on following evaluative judgment behavior increases with the temporal distance between prime and target (SOA), and with the participants’ interoceptive awareness, respectively. These findings suggest that the output of implicit emotional stimulus processing is (partly) embodied, and that the perception of the resulting valence-indicative somatic state provides the agent with information about the value of an event.

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Axelrod, Sofia. "Functional dissection of phagocytosis in Nervous system development and the immune system of Drosophila melanogaster." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16536.

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Phagozyten entfernen apoptotische Zellen während der Entwicklung und beseitigen Pathogene im Immunsystem. Die zugrundeliegenden molekularen und zellulären Mechanismen, insbesondere die Unterschiede zwischen Makrophagen und nicht-professionellen Phagozyten wie Gliazellen, sind weitestgehend unklar. Wir haben neuartige Zellkultur-basierte Assays entwickelt, um 86 Kandidatengene zu testen, die wir aus der Literatur sowie unserem Expressions-Profiling in embryonalen Gliazellen von Drosophila melanogaster zusammengestellt haben. Die Genfunktion wurde durch RNAi herabgesenkt und die Phagozytoseeffizienz wurde mittels FACS untersucht; um die funktionelle Spezifität der Gene zu messen, haben wir nicht nur apoptotische Zellen, sondern auch Bakterien und Beads als „Essen“ angeboten. Mit Hilfe von Null-Mutanten und transgenem RNAi wurden die Ergebnis in vivo validiert. Um die Phagozytose apoptotischer Zellen testen, haben wir untersucht, wie Makrophagen und Gliazellen tote Zellen während der Embryonalentwicklung entfernen, während zur Untersuchung der bakteriellen Phagozytoze adulte Fliegen mit Bakterien infiziert wurden. Unser Screen liefert einen Querschnitt durch die verschiedenen Schritte der Phagozytose. In Bezug auf die Erkennung apoptotischer Zellen finden wir sowohl bekannte als auch neue Akteure für Makrophagen und Gliazellen. Außerdem zeigen wir, dass Vesikeltransport für die Phagozytose apoptotischer Zellen erforderlich ist. Überraschenderweise werden Rezeptoren zur Bakterienerkennung auch für apoptotische Zellen benötigt. Umgekehrt sind Apoptose- Rezeptoren auch für bakterielle Phagozytose notwendig, wodurch eine grundlegende Kreuz-Spezifität zutage tritt. Unsere Arbeit liefert die erste systematische und vergleichende Analyse der verschiedenen Phagozytosearten. Durch die Identifizierung vieler neuer Faktoren legt diese Arbeit den Grundstein für ein mechanistisches Verständnis der Phagozytose von apoptotischen Zellen und Bakterien durch Makrophagen und Gliazellen. Phagocytes remove apoptotic cells during development and eliminate pathogens in the immune system. The underlying molecular and cellular mechanisms, particularly the differences between macrophages and non-professional phagocytes like glia, are not well understood. We used novel cell-based assays to screen phagocytic function of candidate genes assembled from literature and our genome-wide transcription profiling of Drosophila melanogaster embryonic glia. Gene function was knocked-down by RNAi and phagocytic efficiency assessed by flow cytometry; to explore functional specificity, we offered not only bacteria, but also apoptotic cells and beads as ''food''. To validate results in vivo, we analysed glial clearance of apoptotic neurons in embryonic development and immune clearance of bacteria in adult flies using both genetic mutants and transgenic RNAi. Our screen provides a cross section of the different steps of phagocytosis from recognition to engulfment and phagosomal degradation. For the recognition of apoptotic cells, we confirm the involvement of known factors, such as the chaperone Calreticulin and PS-binding Annexin, and identify new players, such as NIMA for macrophage and Megalin for glial corpse clearance. We find components associated with vesicular trafficking including the v-SNARE Synaptobrevin and the cytochrome Cyp4g15 to be required for corpse clearance. Unexpectedly, receptors known for bacterial recognition, such as PGRP-LC and TEP2, are also strongly required for apoptotic clearance. Conversely, receptors previously implicated in apoptotic cell recognition are also required in bacterial clearance (SIMU, Draper), revealing cross-specificity of the system. Our work represents the first systematic and comparative assessment of the molecular repertoire of different types of phagocytosis, and, with the identification of many new players, lays the groundwork for a mechanistic dissection of bacterial and corpse clearance by glia and macrophages.

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31

Daly, Marwa El. "Challenges and potentials of channeling local philanthropy towards development and aocial justice and the role of waqf (Islamic and Arab-civic endowments) in building community foundations." Doctoral thesis, Humboldt-Universität zu Berlin, Philosophische Fakultät III, 2012. http://dx.doi.org/10.18452/16511.

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Diese Arbeit bietet eine solide theoretische Grundlage zu Philanthropie und religiös motivierten Spendenaktivitäten und deren Einfluss auf Wohltätigkeitstrends, Entwicklungszusammenarbeit und einer auf dem Gedanken der sozialen Gerechtigkeit beruhenden Philanthropie. Untersucht werden dafür die Strukturen religiös motivierte Spenden, für die in der islamischen Tradition die Begriffe „zakat“, „Waqf“ oder im Plural auch „awqaf-“ oder „Sadaqa“ verwendet werden, der christliche Begriff dafür lautet „tithes“ oder „ushour“. Aufbauend auf diesem theoretischen Rahmenwerk analysiert die qualitative und quantitative Feldstudie auf nationaler Ebene, wie die ägyptische Öffentlichkeit Philanthropie, soziale Gerechtigkeit, Menschenrechte, Spenden, Freiwilligenarbeit und andere Konzepte des zivilgesellschaftlichen Engagements wahrnimmt. Um eine umfassende und repräsentative Datengrundlage zu erhalten, wurden 2000 Haushalte, 200 zivilgesellschaftliche Organisationen erfasst, sowie Spender, Empfänger, religiöse Wohltäter und andere Akteure interviewt. Die so gewonnen Erkenntnisse lassen aussagekräftige Aufschlüsse über philanthropische Trends zu. Erstmals wird so auch eine finanzielle Einschätzung und Bewertung der Aktivitäten im lokalen Wohltätigkeitsbereich möglich, die sich auf mehr als eine Billion US-Dollar beziffern lassen. Die Erhebung weist nach, dass gemessen an den Pro-Kopf-Aufwendungen die privaten Spendenaktivitäten weitaus wichtiger sind als auswärtige wirtschaftliche Hilfe für Ägypten. Das wiederum lässt Rückschlüsse zu, welche Bedeutung lokale Wohltätigkeit erlangen kann, wenn sie richtig gesteuert wird und nicht wie bislang oft im Teufelskreis von ad-hoc-Spenden oder Hilfen von Privatperson an Privatperson gefangen ist. Die Studie stellt außerdem eine Verbindung her zwischen lokalen Wohltätigkeits-Mechanismen, die meist auf religiösen und kulturellen Werten beruhen, und modernen Strukturen, wie etwa Gemeinde-Stiftungen oder Gemeinde-„waqf“, innerhalb derer die Spenden eine nachhaltige Veränderung bewirken können. Daher bietet diese Arbeit also eine umfassende wissenschaftliche Grundlage, die nicht nur ein besseres Verständnis, sondern auch den nachhaltiger Aus- und Aufbau lokaler Wohltätigkeitsstrukturen in Ägypten ermöglicht. Zentral ist dabei vor allem die Rolle lokaler, individueller Spenden, die beispielsweise für Stiftungen auf der Gemeindeebene eingesetzt, wesentlich zu einer nachhaltigen Entwicklung beitragen könnten – und das nicht nur in Ägypten, sondern in der gesamten arabischen Region. Als konkretes Ergebnis dieser Arbeit, wurde ein innovatives Modell entwickelt, dass neben den wissenschaftlichen Daten das Konzept der „waqf“ berücksichtigt. Der Wissenschaftlerin und einem engagierten Vorstand ist es auf dieser Grundlage gelungen, die Waqfeyat al Maadi Community Foundation (WMCF) zu gründen, die nicht nur ein Modell für eine Bürgerstiftung ist, sondern auch das tradierte Konzept der „waqf“ als praktikable und verbürgte Wohlstätigkeitsstruktur sinnvoll weiterentwickelt. This work provides a solid theoretical base on philanthropy, religious giving (Islamic zakat, ‘ushour, Waqf -plural: awqaf-, Sadaqa and Christian tithes or ‘ushour), and their implications on giving trends, development work, social justice philanthropy. The field study (quantitative and qualitative) that supports the theoretical framework reflects at a national level the Egyptian public’s perceptions on philanthropy, social justice, human rights, giving and volunteering and other concepts that determine the peoples’ civic engagement. The statistics cover 2000 households, 200 Civil Society Organizations distributed all over Egypt and interviews donors, recipients, religious people and other stakeholders. The numbers reflect philanthropic trends and for the first time provide a monetary estimate of local philanthropy of over USD 1 Billion annually. The survey proves that the per capita share of philanthropy outweighs the per capita share of foreign economic assistance to Egypt, which implies the significance of local giving if properly channeled, and not as it is actually consumed in the vicious circle of ad-hoc, person to person charity. In addition, the study relates local giving mechanisms derived from religion and culture to modern actual structures, like community foundations or community waqf that could bring about sustainable change in the communities. In sum, the work provides a comprehensive scientific base to help understand- and build on local philanthropy in Egypt. It explores the role that local individual giving could play in achieving sustainable development and building a new wave of community foundations not only in Egypt but in the Arab region at large. As a tangible result of this thesis, an innovative model that revives the concept of waqf and builds on the study’s results was created by the researcher and a dedicated board of trustees who succeeded in establishing Waqfeyat al Maadi Community Foundation (WMCF) that not only introduces the community foundation model to Egypt, but revives and modernizes the waqf as a practical authentic philanthropic structure.

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32

Wilbert, Niko. "Hierarchical Slow Feature Analysis on visual stimuli and top-down reconstruction." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16526.

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In dieser Dissertation wird ein Modell des visuellen Systems untersucht, basierend auf dem Prinzip des unüberwachten Langsamkeitslernens und des SFA-Algorithmus (Slow Feature Analysis). Dieses Modell wird hier für die invariante Objekterkennung und verwandte Probleme eingesetzt. Das Modell kann dabei sowohl die zu Grunde liegenden diskreten Variablen der Stimuli extrahieren (z.B. die Identität des gezeigten Objektes) als auch kontinuierliche Variablen (z.B. Position und Rotationswinkel). Dabei ist es in der Lage, mit komplizierten Transformationen umzugehen, wie beispielsweise Tiefenrotation. Die Leistungsfähigkeit des Modells wird zunächst mit Hilfe von überwachten Methoden zur Datenanalyse untersucht. Anschließend wird gezeigt, dass auch die biologisch fundierte Methode des Verstärkenden Lernens (reinforcement learning) die Ausgabedaten unseres Modells erfolgreich verwenden kann. Dies erlaubt die Anwendung des Verstärkenden Lernens auf hochdimensionale visuelle Stimuli. Im zweiten Teil der Arbeit wird versucht, das hierarchische Modell mit Top-down Prozessen zu erweitern, speziell für die Rekonstruktion von visuellen Stimuli. Dabei setzen wir die Methode der Vektorquantisierung ein und verbinden diese mit einem Verfahren zum Gradientenabstieg. Die wesentlichen Komponenten der für unsere Simulationen entwickelten Software wurden in eine quelloffene Programmbibliothek integriert, in das ``Modular toolkit for Data Processing'''' (MDP). Diese Programmkomponenten werden im letzten Teil der Dissertation vorgestellt. This thesis examines a model of the visual system, which is based on the principle of unsupervised slowness learning and using Slow Feature Analysis (SFA). We apply this model to the task of invariant object recognition and several related problems. The model not only learns to extract the underlying discrete variables of the stimuli (e.g., identity of the shown object) but also to extract continuous variables (e.g., position and rotational angles). It is shown to be capable of dealing with complex transformations like in-depth rotation. The performance of the model is first measured with the help of supervised post-processing methods. We then show that biologically motivated methods like reinforcement learning are also capable of processing the high-level output from the model. This enables reinforcement learning to deal with high-dimensional visual stimuli. In the second part of this thesis we try to extend the model with top-down processes, centered around the task of reconstructing visual stimuli. We utilize the method of vector quantization and combine it with gradient descent. The key components of our simulation software have been integrated into an open-source software library, the Modular toolkit for Data Processing (MDP). These components are presented in the last part of the thesis.

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33

Wohlfeil, Jürgen. "Optical orientation determination for airborne and spaceborne line cameras." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2012. http://dx.doi.org/10.18452/16506.

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Flugzeug- und satellitengestützte Zeilenkameras ermöglichen eine sehr ökonomische Aufnahme von hoch aufgelösten Luftbildern mit großer Schwadbreite. Eine ungleichförmige Bewegung der Kamera kann sich auf die Bildqualität auswirken. Deswegen ist es unerlässlich, schnelle Orientierungsänderungen der Kamera mit angemessener Genauigkeit und Messrate zu erfassen. Deshalb ist es unerlässlich, die Orientierung der Kamera genau zu messen, um sicher zu stellen, dass die resultierenden Bilder in einem Nachbearbeitungsschritt geometrisch korrigiert werden können. Angemessene High-End-Navigationssysteme sind groß und teuer und ihre Genauigkeit und Messrate dennoch für viele denkbare Anwendungen unzureichend. Aus diesen Gründen besteht ein großes Interesse an Methoden zur Unterstützung der Orientierungsmessung durch die Nutzung optischer Informationen vom Hauptobjektiv bzw. Teleskop. In dieser Arbeit werden zwei unterschiedliche Verfahren vorgestellt, die es erlauben, schnelle Orientierungsänderungen der Kamera auf optischem Wege zu ermitteln. Ersteres basiert auf zusätzlichen Bildsensoren mit kleiner Fläche. Der optische Fluss auf diesen Bildsensoren wird ermittelt und zur Bestimmung von Orientierungsänderungen beliebiger Fernerkundungssysteme verwendet. Das zweite Verfahren beruht ausschließlich auf den Inhalten der Zeilenbilder und der gemessenen Kameratrajektorie. Hierfür macht sich das Verfahren die typische Geometrie multispektraler Zeilenkameras zu Nutze. Zunächst werden homologe Punkte in den möglicherweise stark verzerrten Zeilenbildern unterschiedlicher Spektralbänder extrahiert. Diese Punkte werden dann dazu benutzt, die Orientierungsänderungen der Kamera zu ermitteln. Schließlich wird gezeigt, dass es möglich ist, die absolute Orientierung einer luftgestützten Zeilenkamera anhand der optisch ermittelten Orientierungsänderungen hochgenau zu ermitteln. Airborne and spaceborne line cameras allow a very economic acquisition of high resolution and wide swath images of the Earth. They generate two-dimensional images while rotating or translating, which causes a non-rigid image geometry. Nonuniform motion of the camera can negatively affect the image quality. Due to this, a key requirement for line cameras is that fast orientation changes have to be measured with a very high rate and precision. Therefore, it is essential to measure the camera’s orientation accurately to ensure that the resulting imagery products can be geometrically corrected in a later processing step. Adequate high-end measurement systems are large and expensive and their angular and temporal resolution can be still too low for many possible applications. Due to these reasons there is great interest in approaches to support the orientation measurement by using optical information received through the main optics or telescope. In this thesis two different approaches are presented that allow the determination of a line camera’s orientation changes optically. One approach to determine fast orientation changes is based on small auxiliary frame image sensors. The optical flow on these sensors is determined and used to derive orientation changes of any remote sensing system. The second approach does not require any additional sensors to determine orientation changes. It relies only on the images of a line camera and a rough estimate of its trajectory by taking advantage of the typical geometry of multi-spectral line cameras. In a first step homologous points are detected within the distorted images of different spectral bands. These points are used to calculate the orientation changes of the camera with a high temporal and angular resolution via bundle adjustment. Finally it is shown how the absolute exterior orientation of an airborne line camera can completely be derived from the optically determined orientation changes.

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Clemens, Jan. "Neural computation in small sensory systems." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16556.

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Das Ziel von computational neuroscience ist, neuronale Transformationen zu beschreiben und deren Mechanismen und Funktionen zu beleuchten. Diese Doktorarbeit kombiniert Experiment, Datenanalyse und Modelle um neuronale Kodierung anhand des auditorischen Systems von Feldheuschrecke und Grille zu erforschen. Der erste Teil befasst sich mit der neuronalen Repräsentation von Balzsignalen in Feldheuschrecken. In Rezeptoren ist die Kodierung dieser Signale homogen - alle Neuronen bilden den Reiz gleich ab. In nachgeschalteten Zellen wird die Kodierung spärlicher, sowohl auf Ebene der Zeit als auch der Zellpopulation. Es entsteht ein labeled line code, bei dem unterschiedliche Nervenzellen unterschiedliche Merkmale des Stimulus abbilden. Dieser Transformation liegt eine nichtlineare Kombination von mehreren Stimulusmerkmalen zu Grunde. Die erhöhte Spezifizität von Neuronen dritter Ordnung ermöglicht eine einfache Art der Musterklassifikation, bei der die Zeitpunkte bestimmter Reizelemente innerhalb des Signals ignoriert werden können. Die beschriebene Reiztransformation repräsentiert einen Mechanismus für die Erkennung zeitlich redundanter Kommunikationssignale, wie sie von vielen Insekten produziert werden. Im zweiten Teil wird gezeigt, dass die spektrale und zeitliche Abstimmung von Neuronen zweiter Ordnung bei Grillen von der Komplexität des Reizes abhängt. Während die Abstimmung für Reize mit nur einer Trägerfrequenz breit ist, führen Reize mit mehreren Trägerfrequenzen zu einer Schärfung. Hierdurch kann Information über einzelne Komponenten eines komplexen Signals in der Kodierung erhalten werden. Ein statisches Netzwerkmodell zeigt, dass diese adaptive Abstimmung mit Mechanismen erzeugt werden kann, die in Nervensystemen vieler Organismen vorkommen. Wie diese Doktorabeit zeigt, vereinen Insekten einfach aufgebaute und gut zugängliche Nervensysteme mit komplexen Reiztransformationen. Dies macht sie zu produktiven Modellorganismen für die Neurowissenschaften. The goal of computational neuroscience is to describe the stimulus transformations performed by neural systems and to elucidate their mechanisms and functions. This thesis combines experiment, data analysis and theoretical modeling to explore neural coding in the small auditory systems of grasshoppers and crickets. The first part deals with the transformation of the neural representation of courtship signals in grasshoppers. The code in auditory receptors is relatively homogeneous. That is, all neurons represent a very similar stimulus feature. Representation in higher-order neurons leads to an increase of temporal and population sparseness. This creates a labeled-line population code where different neurons represent different and specific stimulus features. Sparseness in the system increases through a nonlinear combination of two stimulus features. This transformation enables a simple mode of pattern classification, which ignores the timing of individual features and relies only on their average values during a signal. The transformation can therefore facilitate the recognition of the long, temporally redundant communication signals produced by grasshoppers and other insects. The second part shows that spectral and temporal tuning of second-order neurons in crickets strongly depends on the complexity of the stimulus. While tuning is relatively broad for single-carrier stimuli, signals containing multiple carrier frequencies lead to a sharpening of the tuning. This sharpening preserves information about individual components of a complex stimulus. A network model revealed that such adaptive tuning can be implemented in a static network with mechanisms that are ubiquitous in many neural systems. In summary, this study shows that the nervous systems of insects combine a relatively simple structure with complex stimulus transformations. This renders them empirically accessible and suitable model systems for computational neuroscience.

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35

Rolff, Jana. "Charakterisierung von in vivo Modellen des humanen nicht-kleinzelligen Lungenkarzinoms zur Therapieoptimierung." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16515.

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Das Bronchialkarzinom ist die häufigste Todesursache bei den Krebserkrankungen und weist eine schlechte Prognose auf. Die Behandlung besteht aus einer Chemotherapie mit platinbasierten Medikamenten, doch der Erfolg ist unbefriedigend. In den letzten Jahren wurden zielgerichtete Therapien gegen Proteine wie den EGFR entwickelt. Klinische Studien zeigten, dass nur Subpopulationen von den Medikamenten Erlotinib und Cetuximab profitieren. Eine bessere (Vor-)Selektion der Patienten ist wünschenswert, um unnötige Behandlungen zu vermeiden. Für diese Analysen bedarf es relevanter präklinischer Modelle. Im Rahmen dieser Arbeit wurden 25 Xenograftmodelle des Lungenkarzinoms vergleichend charakterisiert. Ein Schwerpunkt bestand im Vergleich der Xenografts mit ihren Patiententumoren. Die Analyse der Histologie, der Proliferationsmarker als auch der Genexpressionsprofile fand übereinstimmende Ergebnisse in den Patiententumoren und ihren abgeleiteten Xenografts. Mit Hilfe von mRNA-, Protein- und SNP-Profilen ressistenzassoziierter Marker der Chemotherapie konnte die Bedeutung der Modelle zur Charakterisierung von prädiktiven und prognostischen Markern aufklärt werden. Diese Arbeit untersuchte auch Marker der anti-EGFR-Therapien. mRNA- und Proteinprofile der ERBB-Rezeptoren sowie der Liganden wurden erstellt und stimmten mit publizierten klinischen Daten überein. Genexpressionsstudien in Erlotinib Respondern und Non-Respondern zur Therapieoptimierung identifizierten den Wachstumsfaktor VEGFA als Ziel für eine Kombinationsbehandlung mit dem Angiogeneseinhibitor Bevacizumab. Die Kombination von Bevacizumab mit Erlotinib führte zu einem reduzierten Tumorwachstum. Die Ergebnisse dieser Arbeit machten deutlich, dass die individuellen Tumoreigenschaften in den patientenabgleiteten Xenografts auf Gen- und Proteinebene erhalten bleiben und diese als Modelle zur Markeranalyse sowie zur Therapieoptimierung eingesetzt werden können. Lung cancer is still one of the most frequent cancers worldwide. The treatment option is classical chemotherapy that is based upon the combination of platin-based drugs. But no further improvement seems to be possible. For some years targeted drugs against single proteins like the EGFR were developed. The clinical trials showed that only subpopulations of patients benefit from the treatment. A better selection of patients to avoid treatment would be helpful. Therefore, pre-clinical models that are suitable for analysis and that represent clinical populations of patients are required. In this work 25 patient derived xenografts from lung cancer were intensely studied. First, the xenografts were compared with their corresponding patient tumor. The analysis of the histology and the expression of proliferation and epithelial or mesenchymal markers showed concordance of the patient tumor and the derived xenograft. The gene expression profiles were also maintained. Further analysis should elucidate the relevance of the xenografts as models for the characterisation and validation of predictive and prognostic markers. SNP, mRNA and protein expression profiles of resistance markers for chemotherapy were generated and showed similarities with clinical data. As marker for the anti-EGFR targeted therapies the ERBB receptors and the ligands of the EGFR were analysed. The mRNA and protein expression profiles resemble clinical data sets. An optimisation of the therapy should be achieved with gene expression studies. The vascular endothelial growth factor A was identified for a combination treatment with the anti-angiogenic drug bevacizumab in erlotinib resistant tumors. The combination of erlotinib and bevacizumab reduced the tumor growth in selected models. In summary, the analysis could show that the individual characteristics of the patient tumor were maintained in the xenograft. The models are a reliable tool for studies designed to improve treatment strategies.

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36

Treue, Denise. "Effekte onkolytischer Adenoviren auf die Aktivität zellulärer Signaltransduktionswege in Gliomzellen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16517.

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Die Therapie des häufigsten primären Hirntumors bei Erwachsenen, des Glioblastoms, gestaltet sich aufgrund der relativen Resistenz gegen Bestrahlung und Zytostatika schwierig und resultiert in einer äußerst schlechten Prognose. Ziel dieser Arbeit war die Identifikation von zellulären Faktoren, die bei einer Behandlung mit dem neuartigen onkolytischen Virus Ad5-Delo3RGD (ΔE1A-13S, ΔE19K, ΔE3, zusätzliches RGD-Motiv) eine Bedeutung hinsichtlich des Ansprechens auf die Therapie haben. Dazu erfolgte nach der Transduktion von Gliomzellen mit adenoviralen Vektoren, mit unterschiedlichem E1A-Status, eine Analyse der Modulation der globalen Genexpression. Die E1A-Deletante, Ad5-Ad312, war replikationsinkompetent in Gliomzellen und hatte infolgedessen nur einen marginalen Einfluss auf Genebene und keine biologischen Effekte auf Proteinebene. Das Wildtypvirus (Ad5-wt) mit intakter und das onkolytische Virus (Ad5-Delo3RGD) mit mutierter E1A-Region induzierten eine starke Modifikation der zellulären Genexpression in Gliomzellen. Die Transduktion der Gliomzellen mit Wildtypvirus bzw. onkolytischen Virus resultierte in einer bis zu 60 %-igen Hemmung der Sekretion des Angiogenesefaktors VEGF. Fernerhin konnte in Glioblastomzellen gezeigt werden, dass Ad5-wt und Ad5-Delo3RGD eine 50 bis 60 %-ige Inhibition der Sekretion von TGF-β2, sowie eine bis zu 65 %-ige Hemmung der Transkriptionsaktivität des TGF-β-Signalwegs induzierten. Damit reprimieren besagte Viren zelluläre Faktoren, deren Expression im Gliom mit einer schlechten Prognose korreliert ist. Aufgrund dieser Daten konnte für maligne Gliome ein Modell von Ad5-Delo3RGD-Therapie relevanten zellulären Faktoren entwickelt werden, welches den Zusammenhang zwischen TGF-β2, VEGF bzw. MIR-181 und der Virotherapie verdeutlicht. Mit Hilfe dieses Modells steigt das Verständnis um die antineoplastische Aktivität des onkolytischen Virus, und ermöglicht damit eine Verbesserung der Ad5-Delo3RGD-basierten Therapie. Glioblastoma is the most common type of brain tumor among adults. The therapy is very difficult, due to its relative resistance to radiation and zytostatica, and often results in fatal prognosis. The main objective of this thesis was the identification of cellular factors associated with therapy response to adenoviral treatment using the newly developed oncolytic virus Ad5-Delo3RGD. Therefore, after viral transduction of glioma cells with adenoviral vectors, using E1A-wildtype and -mutant viruses, an analysis of the modulation of mRNA expression profiles was conducted. The E1A deletion mutant Ad5-Ad312 was replication-deficient in glioma cells and therefore had only marginal influence of host gene transcription level and no biological effect on protein level. Both, wildtype adenovirus type 5 (Ad5-wt), with intact E1A, and oncolytic virus (Ad5-Delo3RGD), with mutated E1A, induced strong modifications of gene expression profiles in glioma cells. The transduction of glioma cells with wildtype or oncolytic virus resulted in a 60 % reduced secretion of the angionesis factor Vascular Endothelial Growth Factor (VEGF). In addition it was shown that Ad5-wt and Ad5-Delo3RGD induced a reduced secretion of TGF-ß2 by 50 to 60 % and a repression of the SMAD2/SMAD3/SMAD4-specific transcription activity up to 65 %. Thus the viruses inhibit cellular factors, which expression is corelated to a weak prognosis. Based on this data a new model for malign glioma was developed, which illustrates the relationship between virotherapy and the cellular factors TGF-β2, VEGF and MIR-181, which are associated with treatment response to Ad5-Delo3RGD-based therapy. The model helps to understand the basic antineoplastic activity of the oncolytic virus and therefore should help to improve Ad5-Delo3RGD-based therapy.

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Dietel, Kristin. "Expression bakterieller Phytasen in Pflanzen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16156.

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Die Verfügbarkeit des Makroelementes Phosphor ist für Lebewesen eingeschränkt. Besonders bei der Pflanzenproduktion und der Tierernährung spielt die Phosphorverfügbarkeit eine wichtige Rolle bei der ökonomischen Nutzung der Ressourcen. In den Fokus der Wissenschaft zur Lösung des Phosphorproblems gerieten die Phytasen, da monogastrische Tiere nicht in der Lage sind das in den Pflanzensamen gespeicherte Phytat zu nutzen. Die gentechnische Veränderung von Pflanzen stellt eine effiziente Möglichkeit zur Produktion von phosphatfreisetzenden Enzymen, zur Erhöhung der Biomasseproduktion und zur Veränderung der Inhaltstoffe dar. In dieser Arbeit wurden erfolgreich transgene Pflanzen der Arten Nicotiana tabacum L. cv. Samsun und Hordeum vulgare L. cv. Golden Promise erzeugt, die in der Lage waren die Phytase aus Klebsiella sp. ASR1 bzw. aus Bacillus amyloliquefaciens FZB45 zu produzieren. Es wurde für jedes Protein eine Strategie zur Reinigung des aktiven Enzyms aus den verschiedenen Wirtsorganismen entwickelt und seine biochemischen Eigenschaften charakterisiert. Es wurde gezeigt, dass die β-Propeller-Phytase aus Bacillus im Gegensatz zur sauren Phytase aus Klebsiella durch die posttranslationale Modifikation teilweise ihre Eigenschaften ändert. Die Auswirkungen der heterologen Expression der Phytasen auf die Veränderung der Anteile von Phytinsäure und anorganischem Phosphor, in Relation zum gesamten Phosphor, in den Gerstensamen wurden untersucht. Es wurde eine Reduktion des Phytinsäuregehaltes um 19 % und eine Erhöhung des Gehalts anorganischem Phosphor zwischen 27 % und 78 nachgewiesen. Mit Hilfe von spezifischen Signalsequenzen gelang es die Phytaseproteine aus der Wurzel in das umgebende Medium zu sekretieren. Die Sekretion der Bacillus Phytase führte zu einer Steigerung der Biomasseproduktion von Nicotiana tabacum L. unter unsterilen Wachstumsbedingungen mit Phytat als einziger P-Quelle um 34 %. Due to the bad availability of phosphorus in natural habitats the improvement of phosphorus accessibility to organisms became an important topic of research, particularly for agriculture and animal nutrition. In plant seeds phosphorus is bound to D-myo-inositol to form phytic acid that is indigestible for mono gastric animals. Therefore the use of phytases to hydrolyze the phytic acid and to mobilize the anorganic phosphorus came in focus to science. Genetic engineering gave the opportunity to improve the phosphorus availability. Genetic manipulation of plants is a suitable tool to produce phosphorus releasing enzymes, thereby increasing the biomass and decreasing the content of phytic acid in plants. In this work I generated transgenic plants of Nicotiana tabacum and Hordeum vulgare vulgare which successfully express the phytase gene phyK from Klebsiella pneumonia ASR1 and phyC from Bacillus amyloliquefaciens FZB42. Chromatographically purification strategies were developed and biochemical properties were characterized for all phytase proteins. All enzymes were active and PhyC was posttranslational modified. The effect of the recombinant phytase activity to total phosphorus, phytic acid and anorganic phosphorus content of barley seeds were elucidated. The phyK-expression in barley seeds yield a reduction of phytic acid content of about 19 % and an increase of anorganic phosphorus of about 78 %. Transgenic barley seeds with phyC gene expression show an increase of anorganic phosphorus content from 27 % to 48 % but no decrease in phytic acid content. In growth experiments no special phenotypes of plant containing the recombinant protein were visible. The insertion of apoplastic signal sequences in front of the phytase genes resulted in secretion of the proteins into the rhizosphere. The secretion of PhyC led to an improved growth of Nicotiana tabacum under unsterile conditions with sodium phytate as single phosphorus source increasing the biomass up to 34 %.

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38

Leddin, Mathias. "Unterschiedliche Autoregulation am PU.1 Lokus in B -Zellen und myeloischen Zellen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16546.

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Als Schlüsselfaktor des hämatopoietischen Systems spielt PU.1 eine ent-scheidende Rolle in der Entwicklung der meisten hämatopoietischen Li-nien. Das PU.1 Expressionslevel bestimmt das Differenzierungspotential hämatopoietischer Stammzellen und Vorläufer. In den unterschiedlichen Zelltypen werden verschiedene Expressionsstärken etabliert. Wie diese zelltypischen Expressionslevel vonPU.1 generiert werden, ist bisher weit-gehend unbekannt. In der vorliegenden Doktorarbeit wurde mit Hilfe eines transgenen Maus-modells die cis-regulatorische Einheit von PU.1 definiert, um mit nachfol-genden molekularbiologischen und genomweiten Ansätzen Mechanismen der zellspezifischen Regulation von PU.1 zu aufzuzeigen. Die Definition der cis-regulatorischen Einheit von PU.1 erfolgte mit Hilfe eines transgenen Mausmodells, welches ein humanes PU.1 BAC Kons-trukt trägt. Es konnte gezeigt werden, dass humanes und murines PU.1 substituierbar sind und den gleichen Regulationsmechanismen unterlie-gen. Mit Hilfe genomweite DNaseI hypersensitivity Analysen, Methylie-rungs- und Bindungsstudien konnte ein neuer Regulationsmechanismus beschrieben werden, der eine spezifische kombinatorische Interaktion verschiedener cis-regulatorischer Elemente erfordert. Durch Reportergenassays in verschiedenen Zelltypen war es möglich, einen myeloischen Enhancer zu identifizieren. Es konnte gezeigt werden, dass PU.1 mit zelltyp-spezifischen Transkriptionsfaktoren interagiert, um unterschiedliche Bindungsmuster an seinen regulatorischen Elementen zu etablieren. Dadurch kommt es zu den spezifischen Expressionstärken von PU.1 The transcription factor PU.1 occupies a central role in controlling myeloid and early B cell development and its correct lineage-specific expression is critical for the differentiation choice of hematopoietic progenitors. However, little is known of how this tissue-specific pattern is established. We previously identified an upstream regulatory cis-element (URE) whose targeted deletion in mice decreases PU.1 expression and causes leukemia. We show here that the URE alone is insufficient to confer physiological PU.1 expression, but requires the cooperation with other, previously unidentified elements. Using a combination of transgenic studies, global chromatin assays and detailed molecular analyses we present evidence that PU.1 is regulated by a novel mechanism involving cross-talk between different cis-elements together with lineage-restricted autoregulation. In this model, PU.1 regulates its expression in B cells and macrophages by differentially associating with cell-type specific transcription factors at one of its cis-regulatory elements to establish differential activity patterns at other elements.

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39

Tarasca, Nicola. "Geometric cycles on moduli spaces of curves." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2012. http://dx.doi.org/10.18452/16518.

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Ziel dieser Arbeit ist die explizite Berechnung gewisser geometrischer Zykel in Modulräumen von Kurven. In den letzten Jahren wurden Divisoren auf $\Mbar_{g,n}$ ausgiebig untersucht. Durch die Berechnung von Klassen in Kodimension 1 konnten wichtige Ergebnisse in der birationalen Geometrie der Räume $\Mbar_{g,n}$ erzielt werden. In Kapitel 1 geben wir einen Überblick über dieses Thema. Im Gegensatz dazu sind Klassen in Kodimension 2 im Großen und Ganzen unerforscht. In Kapitel 2 betrachten wir den Ort, der im Modulraum der Kurven vom Geschlecht 2k durch die Kurven mit einem Büschel vom Grad k definiert wird. Da die Brill-Noether-Zahl hier -2 ist, hat ein solcher Ort die Kodimension 2. Mittels der Methode der Testflächen berechnen wir die Klasse seines Abschlusses im Modulraum der stabilen Kurven. Das Ziel von Kapitel 3 ist es, die Klasse des Abschlusses des effektiven Divisors in $\Mbar_{6,1}$ zu berechnen, der durch punktierte Kurven [C, p] gegeben ist, für die ein ebenes Modell vom Grad 6 existiert, bei dem p auf einen Doppelpunkt abgebildet wird. Wie Jensen gezeigt hat, erzeugt dieser Divisor einen extremalen Strahl im pseudoeffektiven Kegel von $\Mbar_{6,1}$. Ein allgemeines Ergebnis über gewisse Familien von Linearsystemen mit angepasster Brill-Noether-Zahl 0 oder -1 wird eingeführt, um die Berechnung zu vervollständigen. The aim of this thesis is the explicit computation of certain geometric cycles in moduli spaces of curves. In recent years, divisors of $\Mbar_{g,n}$ have been extensively studied. Computing classes in codimension one has yielded important results on the birational geometry of the spaces $\Mbar_{g,n}$. We give an overview of the subject in Chapter 1. On the contrary, classes in codimension two are basically unexplored. In Chapter 2 we consider the locus in the moduli space of curves of genus 2k defined by curves with a pencil of degree k. Since the Brill-Noether number is equal to -2, such a locus has codimension two. Using the method of test surfaces, we compute the class of its closure in the moduli space of stable curves. The aim of Chapter 3 is to compute the class of the closure of the effective divisor in $\M_{6,1}$ given by pointed curves [C,p] with a sextic plane model mapping p to a double point. Such a divisor generates an extremal ray in the pseudoeffective cone of $\Mbar_{6,1}$ as shown by Jensen. A general result on some families of linear series with adjusted Brill-Noether number 0 or -1 is introduced to complete the computation.

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40

Huber, Frank. "Anfragebearbeitung auf Mehrkern-Rechnerarchitekturen." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2012. http://dx.doi.org/10.18452/16512.

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Der Trend zu immer mehr parallelen Recheneinheiten innerhalb eines Prozessors stellt an die Softwareentwicklung neue Herausforderungen. Um die vorhandenen Ressourcen auszulasten und die stetige Steigerung der Parallelität in einen Leistungszuwachs umzusetzen, muss Software von der sequentiellen Verarbeitung in eine hochgradig parallele Verarbeitung übergehen. Diese Arbeit untersucht, wie solch eine parallele Verarbeitung in Bezug auf Relationale Datenbankmanagementsysteme umzusetzen ist. Dazu wird zunächst der gesamte Prozess der Anfragebearbeitung betrachtet und vier Problembereiche identifiziert, die für das Ziel der parallelen Anfragebearbeitung auf Mehrkern-Rechnerarchitekturen maßgeblich sind. Diese Bereiche sind die Hardware selbst, das physische Datenmodell sowie die Anfrageausführung und -optimierung. Diese vier Bereiche werden innerhalb eines Rahmenwerkes betrachtet. Nach einer Einführung, wird sich die Arbeit zunächst mit Grundlagen befassen. Dazu werden die Hardwarebestandteile Speicher und Prozessor betrachtet und ihre Funktionsweise erläutert. Auf diesem Wissen aufbauend, wird ein Hardwaremodell definiert. Es ermöglicht eine von der jeweiligen Hardwarearchitektur unabhängige Softwareentwicklung, ohne den Verlust an Funktionalität und Leistung. Im Weiteren wird das physische Datenmodell untersucht und analysiert, wie das physische Datenmodell eine optimale Anfrageausführung unterstützen kann. Die verwendeten Datenstrukturen müssen dafür einen effizienten und parallelen Zugriff erlauben. Die Analyse führt zur Entwicklung eines neuartigen Indexes, der die datenparallele Abarbeitung nutzt. Gefolgt wird dieser Teil von der Anfrageausführung, in der ein neues Anfrageausführungsmodell entwickelt wird, das auf der Verwendung des Taskkonzepts beruht und eine hohe und sehr leicht gewichtige Parallelität erlaubt. Den Abschluss stellt die Anfrageoptimierung dar, worin verschiedene Ideen für die Optimierung der Ressourcenverwaltung präsentiert werden. The upcoming generation of many-core architectures poses several new challenges for software development: Software design and software implementation has to change from sequential execution to a highly parallel execution, such that it takes full advantage of the steadily growing number of cores on a single processor. With this thesis, we investigate such highly parallel program execution in the context of relational database management systems (RDBMSs). We consider the complete process of query processing and identify four problem areas which are crucial for efficient parallel query processing on many-core architectures. These four areas are: Hardware, physical data model, query execution, and query optimization. Furthermore, we present a framework which covers all four parts, one after another. First, we give a detailed survey of computer hardware with a special focus on memory and processors. Based on this survey we propose a hardware model. Our abstraction aims to simplify the task of software development on many-core hardware. Based on the hardware model, we investigate physical data models and evaluate how the physical data model may support optimal query execution by providing efficient and parallelizable data structures. Additionally, we design a new index structure that utilizes data parallel execution by using SIMD operations. The next layer within our framework is query execution, for which we present a new task based query execution model. Our query execution model allows for a lightweight parallelism. Finally, we cover query optimization by explaining approaches for optimizing resource utilization on a query local point of view as well as query global point of view.

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Sadykova, Saltanat. "Electric microfield distributions and structure factors in dense plasmas." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16316.

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Die elektrischen Mikrofeldverteilungen (EMDs) und ihre Auswüchse wurden in einkomponentiger (OCP) Elektron-, zweikomponentigen (TCP) Elektron-Positron-, Wasserstoff- und einwertig ionisierten Alkaliplasmen im Rahmen verschiedener Pseudopotentialmodelle (PM) untersucht und mit sowohl Molekulardynamik (MD) und Monte-Carlo Simulationen als auch mit Experimenten vergliechen. Die verwendeten theoretischen Verfahren zur Berechnung von EMDs gehen zurück auf die von C. A. Iglesias entwickelte Kopplungsparameter Integrationstechnik (KPIT) für OCP und die von J. Ortner et al. vorgeschlagene verallgemeinerte KPIT für TCP. EMDs wurden im Rahmen der abgeschirmten Kelbg-, Deutsch-, Hellmann-Gurskii-Krasko(HGK)-PM untersucht, welche quantenmechanische Effekte, Abschirmungseffekte und die Struktur der Ionenrümpfe (HGK) berücksichtigen. Die Abschirmungseffekte wurden auf Grundlage der Bogoljubov-Born-Green-Kirkwood-Yvon- Methode eingeführt. Wir haben das abgeschirmte HGK-Pseudopotential in der Debye-Näherung sowie in einer mäßig gekoppelten Plasma-Näherung verwendet. Wir haben verschiedene Typen vom asymptotischen Verhalten der Verteilungsauswüchse in Abhangigheit von Plasmaparameter, Plasmatypen und Strahler bestimmt. Der Vergleich der experimentell gewonnenen Daten mit sowohl einem synthetischen Li2+-Lyman-Spektrum als auch mit einer synthetischen Li II 548 nm Linie lassen den Schluss zu, daß die EMD, welche auf der Grundlage der Iglesias-Methode für OCP im HGK-PM und der MD erhalten wurde, eine gute Übereinstimmung mit den experimentellen Werten liefert. Die statischen partiellen und Ladung-Ladung-Strukturfaktoren (SSF) wurden für Alkali- und Be2+-Plasmen unter Verwendung der von G. Gregori et al. beschriebenen Methode berechnet. Die dynamischen Strukturfaktoren (DSF) für Alkaliplasmen wurden unter Verwendung der durch V. M. Adamyan et al. entwickelten Methode der Momente berechnet. Bei beiden Methoden wurde das abgeschirmte HGK-Pseudopotential verwendet. The electric microfield distributions (EMDs) and its tails have been studied for electron one-component plasma (OCP), electron-positron, hydrogen and single-ionized alkali two-component plasmas (TCP) in a frame of different pseudopotential models (PM) and compared with Molecular Dynamics (MD) and Monte-Carlo simulations as well as with experiments. The theoretical methods used for calculation of EMDs are a coupling-parameter integration technique (CPIT) developed by C. A. Iglesias for OCP and the generalized CPIT proposed by J. Ortner et al. for TCP. We studied the EMDs in a frame of the screened Kelbg, Deutsch, Hellmann-Gurskii-Krasko (HGK) PMs which take into account quantum-mechanical, screening effects and the ion shell structure (HGK) due to the Pauli exclusion principle. The screening effects were introduced on a base of Bogoljubov-Born-Green-Kirkwood-Yvon method. We used the screened HGK pseudopotential in the Debye approximation as well as in a moderately coupled plasma approximation. The influence of the plasma coupling parameter on the EMD along with the ion shell structure was investigated. We determined different types of asymptotic behaviour of EMD tails in dependence on the plasma type, parameters and radiator. Comparison of a synthetic Li2+ Lyman spectrum as well as comparison of a synthetic Li II 548 nm line with experimental data allows us to conclude that the EMD, obtained on a base of the CPIT method for OCP within the HGK PM and MD, provides a good agreement with the experiment. We have calculated the partial and charge-charge static structure factors (SSF) for alkali and Be2+ plasmas using the method described by G. Gregori et al.. We have calculated the dynamic structure factors (DSF) for alkali plasmas using the method of moments developed by V. M. Adamyan et al. In both methods the screened HGK pseudopotential has been used.

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42

Jülke, Kerstin. "Role of cytokines for NK cell competence and differentiation." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2010. http://dx.doi.org/10.18452/16216.

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Humane NK Zellen können in CD56br und CD56dim NK Zellen unterteilt werden. In dieser Arbeit wurde untersucht, in welchem Zusammenhang die verschiedenen NK Zell Populationen stehen und wie funktional kompetente NK Zellen generiert werden. Des Weiteren wurde die Heterogenität der CD56dim NK Zell Population in Bezug auf Funktionalität und Differenzierungsstadien analysiert. Es konnte gezeigt werden, dass CD56br NK Zellen in CD56dim NK Zellen differenzieren. Währenddessen werden u.a. MHC-I spezifische inhibierende Rezeptoren (KIR) erworben. Diese sind essentiell für die Unterscheidung zwischen “Selbst” und “Nicht-Selbst”, wobei nur NK Zellen, die Selbst-MHC-spezifische KIRs tragen, funktional kompetent sind. In der vor-liegenden Arbeit konnte darüber hinaus gezeigt werden, dass zuvor anerge NK Zellen nach Zytokin-induzierter Expression eines Selbst-MHC-spezifischen KIRs kompetent werden. Ex vivo Analysen humaner Gewebe lassen vermuten, dass diese Prozesse während einer Entzündung in sekundären lymphatischen Organen (SLO) stattfinden könnten. Auch CD56dim NK Zellen selbst sind nicht homogen, hingegen können anhand der Expression von KIRs oder CD62L, welches für die Migration in SLO wichtig ist, weitere Subpopulationen unterschieden werden. Eine umfassende Analyse bezüglich KIR und CD62L Expression führte zur Identifizierung einer zuvor nicht charakterisierten CD56dimCD62L+ NK Zell Population, welche die Fähigkeiten von CD56br, Zytokine zu produzieren und zu proliferieren, mit einem hohen zytotoxischen Potenzial, vereinigt. Weitere ex vivo Untersuchungen des Phänotyps, der Telomerlängen und der Verteilung in Relation zum Alter lassen vermuten, dass die Differenzierung humaner NK Zellen von CD56br über CD56dimCD62L+ zu CD56dimCD62L- verläuft, wobei die Zellen mit fortschreitender Dif-ferenzierung ihre Fähigkeit auf Zytokine zu antworten verlieren und dafür die Fähigkeit er-langen, über aktivierende Rezeptoren stimuliert zu werden. Human NK cells comprise two main subsets, CD56br and CD56dim cells. In this study, an extensive analysis of human NK cell phenotype and functional characteristics has been performed in order to investigate the developmental relation between NK cell subsets, to elucidate how NK cell competence is acquired and to further dissect the heterogeneity of the CD56dim subset with regard to functions and differentiation history of human NK cells. It could be shown that upon cytokine activation, CD56br differentiate into CD56dim NK cells and that this process might take place in inflamed secondary lymphoid organs (SLO). One of the crucial markers acquired during this process is KIR, the main MHC-specific inhibitory receptors responsible for self versus non self recognition. Previously, it has been shown that only cells expressing self-MHC specific KIRs are responsive to activating stimuli. In this study, it was demonstrated that induction of self-MHC specific KIR by cytokines leads to acquisition of functional competence. Ex vivo analysis of human tissues suggests that acquisition of KIR and consequently of cytotoxic competence may occur in inflamed SLO. Finally, it was demonstrated that CD56dim NK cells do not represent a homogenous population. When dissected for CD62L and KIR expression, a new subset of NK cells could be identified, namely CD56dimCD62L+, which uniquely combines properties of CD56br NK cells, particularly high IFN-g production upon cytokine stimulation, proliferation and potential to migrate into SLO, with the capacity of CD56dim to kill, produce cytokines upon activating receptor stimulation and to migrate into inflamed tissues. Ex vivo analysis of the function, phenotype, telomere length and frequencies during ageing of CD56br, CD56dimCD62L+ and CD56dimCD62L- NK cells suggest that CD56dimCD62L+ cells represent an intermediate stage of NK cell maturation between the more immature CD56br and the terminally differentiated CD56dim CD62L- NK cells.

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43

Ried, Stefan. "Essays on macroeconomic theory as a guide to economic policy." Doctoral thesis, Humboldt-Universität zu Berlin, Wirtschaftswissenschaftliche Fakultät, 2009. http://dx.doi.org/10.18452/16016.

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Die vorliegende Dissertation zu makroökonomischen Themen beinhaltet einen einleitenden Literaturüberblick, drei eigenständige und voneinander unabhängige Kapitel sowie einen technischen Anhang. In Kapitel zwei wird ein Zwei-Länder Modell einer Währungsunion betrachtet, in dem die gemeinsame Zentralbank die Wohlfahrt der gesamten Währungsunion maximieren will, während die zwei fiskalpolitischen Akteure vergleichbare, aber minimal abweichende länderspezifische Verlustfunktionen zu minimieren suchen. Das Konkurrenzverhalten dieser drei Institutionen wird in sieben spieltheoretischen Szenarien analysiert. Beim Vergleich einer homogenen mit einer heterogenen Währungsunion lassen sich für letztere deutlich höhere Wohlfahrtsverluste relativ zum sozialen Optimum feststellen. Die Szenarien mit den geringsten Wohlfahrtsverlusten sind Kooperation aller drei Institutionen und eine Stackelberg-Führerschaft der Zentralbank. Kapitel drei untersucht, inwieweit das Verhältnis von Immobilienpreise zum Bruttoinlandsprodukt als langfristig konstant und nur auf Grund von Produktivitätsschocks von seinem Mittelwert abweichend angesehen werden kann. Hierzu wird ein Zwei-Sektoren RBC-Modell für den Immobiliensektor und einen Konsumgütersektor erstellt. Es wird gezeigt, dass ein antizipierter, zukünftiger Schock auf das Produktivitätswachstum im Konsumgütersektor eine sofortige, deutliche Erhöhung der Immobilienpreise relativ zum Bruttoinlandsprodukt zur Folge hat. In Kapitel vier wird gefragt, ob ein typisches Neukeynesianisches Modell "sechs große Rätsel der internationalen Makroökonomie" erklären kann. Die sechs Rätsel werden in Bedingungen für erste und zweite Momente übersetzt und fünf wesentliche Modellparameter geschätzt. Das Ergebnis ist erstaunlich gut: unter anderem können die empirischen Beobachtungen zur Heimatpräferenz wiedergegeben und die Schwankungsbreite des realen Wechselkurses deutlich erhöht werden. Handelskosten sind für dieses Ergebnis ein wesentlicher Faktor. This dissertation consists of an introductory chapter with an extended literature review, three chapters on individual and independent research topics, and an appendix. Chapter 2 uses a two-country model with a central bank maximizing union-wide welfare and two fiscal authorities minimizing comparable, but slightly different country-wide losses. The rivalry between the three authorities is analyzed in seven static games. Comparing a homogeneous with a heterogeneous monetary union, welfare losses relative to the social optimum are found to be significantly larger in a heterogeneous union. The best-performing scenarios are cooperation between all authorities and monetary leadership. The goal of Chapter 3 is to investigate whether or not it is possible to explain the house price to GDP ratio and the house price to stock price ratio as being generally constant, deviating from its respective mean only because of shocks to productivity? Building a two-sector RBC model for residential and non-residential capital, it is shown that an anticipated future shock to productivity growth in the non-residential sector leads to an immediate large increase in house prices relative to GDP. In Chapter 4, it is asked whether a typical New Keynesian Open Economy Model is able to explain "Six Major Puzzles in International Macroeconomics". After translating the six puzzles into moment conditions for the model, I estimate five parameters to fit the moment conditions implied by the data. Given the simplicity of the model, its fit is surprisingly good: among other things, the home bias puzzles can easily be replicated, the exchange rate volatility is formidably increased and the exchange rate correlation pattern is relatively close to realistic values. Trade costs are one important ingredient for this finding.

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44

Walter, Sebastian. "Structured higher-order algorithmic differentiation in the forward and reverse mode with application in optimum experimental design." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät II, 2012. http://dx.doi.org/10.18452/16514.

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In dieser Arbeit werden Techniken beschrieben, die es erlauben (höhere) Ableitungen und Taylorapproximationen solcher Computerprogramme effizient zu berechnen. Auch inbesondere dann, wenn die Programme Algorithmen der numerischen linearen Algebra (NLA) enthalten. Im Gegensatz zur traditionellen algorithmischen Differentiation (AD), bei der die zugrunde liegenden Algorithmen um zusätzliche Befehlere erweitert werden, sind in dieser Arbeit die Zerlegungen durch definierende Gleichungen charakterisiert. Basierend auf den definierenden Gleichungen werden Strukturausnutzende Algorithmen hergeleitet. Genauer, neuartige Algorithmen für die Propagation von Taylorpolynomen durch die QR, Cholesky und reell-symmetrischen Eigenwertzerlegung werden präsentiert. Desweiteren werden Algorithmen für den Rückwärtsmodus der AD hergeleitet, welche im Wesentlichen nur die Faktoren der Zerlegungen benötigen. Im Vergleich zum traditionellen Ansatz, bei dem alle Zwischenergebnisse gespeichert werden, ist dies eine Reduktion von O(N^3) zu O(N^2) für Algorithmen mit O(N^3) Komplexität. N ist hier die Größe der Matrix. Zusätzlich kann bestehende, hoch-optimierte Software verwendet werden. Ein Laufzeitvergleich zeigt, dass dies im Vergleich zum traditionellen Ansatz zu einer Beschleunigung in der Größenordnung 100 führen kann. Da die NLA Funktionen als Black Box betrachtet werden, ist desweiteren auch der Berechnungsgraph um Größenordnungen kleiner. Dies bedeutet, dass Software, welche Operator Overloading benutzt, weniger Overhead hervorruft und auch weniger Speicher benötigt. This thesis provides a framework for the evaluation of first and higher-order derivatives and Taylor series expansions through large computer programs that contain numerical linear algebra (NLA) functions. It is a generalization of traditional algorithmic differentiation (AD) techniques in that NLA functions are regarded as black boxes where the inputs and outputs are related by defining equations. Based on the defining equations, structure-exploiting algorithms are derived. More precisely, novel algorithms for the propagation of Taylor polynomials through the QR, Cholesky,- and real-symmetric eigenvalue decomposition are shown. Recurrences for the reverse mode of AD, which require essentially only the returned factors of the decomposition, are also derived. Compared to the traditional approach where all intermediates of an algorithm are stored, this is a reduction from O(N^3) to O(N^2) for algorithms with O( N^3) complexity. N denotes the matrix size. The derived algorithms make it possible to use existing high-performance implementations. A runtime comparison shows that the treatment of NLA functions as atomic can be more than one order of magnitude faster than an automatic differentiation of the underlying algorithm. Furthermore, the computational graph is orders of magnitudes smaller. This reduces the additional memory requirements, as well as the overhead, of operator overloading techniques to a fraction.

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Chan, Kit-i., and 陳潔儀. "Wei Yijie (1616-1686)." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B31691973.

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46

Selighini, Sofia. "Il sessismo linguistico in Spagna: proposta di traduzione di tre columnas sul caso portavozas." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/16016/.

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Il presente elaborato si propone di analizzare la questione del sessismo linguistico nella lingua spagnola. Per farlo, viene presentato un approfondimento del caso portavozas, ovvero di un episodio di sdoppiamento al femminile che ha visto protagonista la deputata Irene Montero in una conferenza stampa del Congresso spagnolo, in data 7 febbraio 2018. Viene inoltre presentata un’analisi della situazione del sessismo linguistico in Spagna e in Italia e dell’evoluzione del linguaggio inclusivo nei due Paesi. La colonna portante dell’elaborato è la proposta di traduzione di tre columnas, articoli di opinione del quotidiano nazionale El País, inerenti alla questione e ritenuti particolarmente interessanti da un punto di vista contenutistico e traduttologico. La ricollocazione editoriale dei testi prevista è la sezione Opinioni della rivista italiana Internazionale. Prima della traduzione di ogni articolo in questione è stata svolta un’analisi pre-traduttiva seguendo il modello funzionale proposto da Christiane Nord nel 2012, il che ha permesso di individuare i principali problemi intratestuali ed extratestuali da tenere in considerazione nel processo traduttivo.

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Ferrara, Camilla. "La Tomografia Computerizzata di ultima generazione per indagini diagnostiche delle arterie coronarie." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/16510/.

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La tesi contiene una descrizione del sistema cardiovascolare e del circolo coronarico seguita dalla presentazione delle patologie delle arterie coronarie con riferimento alle cause, ai fattori di rischio e all'approccio terapeutico con cui vengono trattate. Successivamente viene descritta la procedura di coronarografia di cui si evidenziano le possibili complicazioni. Si introduce poi tomografia computerizzata di cui di presentano il principio di funzionamento, il processo di ricostruzione dell'immagine e come tali apparecchiature si siano evolute nel tempo fino a giungere ai tomografi di ultima generazione. Infine vengono presi in considerazione alcuni test clinici condotti presso il Centro Cardiologico Monzino di Milano, ovvero la struttura che in Italia è in possesso delle tecnologie di ultima generazione. I risultati prodotti da tali test sono stati analizzati con il fine di validare l'impiego di queste nuove apparecchiature come mezzo diagnostico in caso di sospetta coronaropatia, in sostituzione alla coronarografia ritenuta più invasiva. Si è potuto concludere che l'utilizzo di tomografi di ultima generazione determina un gran numero di vantaggi per il paziente tra cui soprattutto una maggiore qualità dell'immagine, una riduzione dei tempi di acquisizione e una ridotta dose efficace.

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Mordenti, Eugenio. "Comportamento a fatica dell’acciaio Maraging MS1 prodotto tramite DMLS: influenza della posizione nella camera." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/16511/.

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L’obiettivo principale di questo studio è caratterizzare dei provini, prodotti mediante additive manufacturing, con il metodo DMLS (direct metal laser sintering), a fatica mediante prove di flessione rotante. I provini sono realizzati in Maraging steel, le cui caratteristiche meccaniche e composizione sono di seguito enunciate. I dati sperimentali ottenuti dalle prove vengono poi elaborati tramite la normativa ISO 12107-2012, per trovare la curva di fatica S-N, sia usando un’interpolazione lineare, che una quadratica, per confrontarle in modo da identificare quale metodologia approssima meglio i dati. E' inoltre utilizzata la normativa Dixon tramite uno stair-caise per trovare la tensione limite di fatica. Un altro aspetto rilevante di questo elaborato è quello di analizzare il processo produttivo dei provini per capire quali sono i principali parametri che influenzano l’additive manufacturing e che conseguenze hanno sulla vita a fatica dei componenti realizzati. Per questo studio sono stati utilizzati tre set di provini (ogni set ha numerosità pari a 12) numerati dal 20 al 22. I set vengono trattati termicamente, lavorati alle macchine utensili, pallinati e sono analizzati nel dettaglio i processi appena citati. Legato a questi studi c’è il tentativo di trovare una correlazione tra la posizione di realizzazione dei provini in camera e la vita a fatica. Infine si esegue un’analisi frattografica per capire meglio i meccanismi di innesco e propagazione delle cricche per fatica.

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Ricci, Iacopo. "La Combinatoria Algebrica delle Funzioni Simmetriche." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/16416/.

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In questa tesi presentiamo lo studio delle funzioni simmetriche dal punto di vista combinatorio, soffermandoci sulle funzioni di Shur e accennando delle applicazioni nella teoria delle rappresentazioni

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Tondini, Fabio. "Resistenza a fatica dell’acciaio Maraging MS1 prodotto tramite DMLS: effetto dei trattamenti post-processo e della posizione in camera." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amslaurea.unibo.it/16556/.

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Determinazione del limite di fatica e delle curve S-N di fatica, realizzate sottoponendo provini di acciaio Maraging MS1, realizzati dalla ditta tedesca EOS mediante additive manufacturing dalla macchina per DMLS, EOSINT M280, a prove di flessione rotante pure. Per l’elaborazione dati è stata seguita la normativa ISO 12107, al fine di stimare il limite di fatica e le bande di confidenza delle curve S-N ottenute sperimentalmente; sono, inoltre, stati presi in considerazione la posizione in camera di realizzazione e i trattamenti post-processo come il trattamento termico, la pallinatura e lavorazione in macchina, per osservare come questi possano influire sulle proprietà meccaniche e la vita a fatica del componente ottenuto. Infine grazie alle analisi frattografiche, è stato possibile individuare la causa della rottura e la propagazione della cricca lungo le superfici di frattura dei provini.

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Dissertations / Theses on the topic 'EN 16516'

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