Relevant bibliographies by topics / Endonucleasi

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Endonucleasi'

Author: Grafiati
Published: 9 March 2023
Last updated: 31 July 2025

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Journal articles on the topic "Endonucleasi"

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Fahmi, Tariq, Xiaoying Wang, Dmitry D. Zhdanov, et al. "DNase I Induces Other Endonucleases in Kidney Tubular Epithelial Cells by Its DNA-Degrading Activity." International Journal of Molecular Sciences 21, no. 22 (2020): 8665. http://dx.doi.org/10.3390/ijms21228665.

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Endonuclease-mediated DNA fragmentation is both an immediate cause and a result of apoptosis and of all other types of irreversible cell death after injury. It is produced by nine enzymes including DNase I, DNase 2, their homologs, caspase-activated DNase (CAD) and endonuclease G (EndoG). The endonucleases act simultaneously during cell death; however, regulatory links between these enzymes have not been established. We hypothesized that DNase I, the most abundant of endonucleases, may regulate other endonucleases. To test this hypothesis, rat kidney tubular epithelial NRK-52E cells were trans
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Kamisugi, Y., Y. Ikeda, M. Ohno, M. Minezawa, and K. Fukui. "In situ digestion of barley chromosomes with restriction endonucleases." Genome 35, no. 5 (1992): 793–98. http://dx.doi.org/10.1139/g92-121.

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In situ digestion of barley chromosomes with restriction endonucleases was examined. All the treatments with five restriction endonucleases, MboII, RsaI, HaeIII, HinfI, and DraII, showed various band patterns on the barley chromosomes. Differences were observed in the band patterns produced with different restriction endonucleases. Uneven staining patterns, similar to the band patterns by the endonuclease treatments, also appeared when the chromosomes were treated with the buffer solution without the enzyme. The band patterns observed both with and without the endonucleases were classified int
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Petersen, Kamilla Vandsø, Cinzia Tesauro, Marianne Smedegaard Hede, et al. "Rolling Circle Enhanced Detection of Specific Restriction Endonuclease Activities in Crude Cell Extracts." Sensors 22, no. 20 (2022): 7763. http://dx.doi.org/10.3390/s22207763.

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Restriction endonucleases are expressed in all bacteria investigated so far and play an essential role for the bacterial defense against viral infections. Besides their important biological role, restriction endonucleases are of great use for different biotechnological purposes and are indispensable for many cloning and sequencing procedures. Methods for specific detection of restriction endonuclease activities can therefore find broad use for many purposes. In the current study, we demonstrate proof-of-concept for a new principle for the detection of restriction endonuclease activities. The m
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Shammas, Masood A., Hemant Koley, Sima Shah, et al. "Dysregulated Apurinic/Apyrimidinic Endonucleases (Ape1 and Ape2) Lead to Genetic Instability in Multiple Myeloma." Blood 104, no. 11 (2004): 1418. http://dx.doi.org/10.1182/blood.v104.11.1418.1418.

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Abstract Multiple myeloma (MM) is associated with significant genomic instability. Homologous recombination (HR), which is elevated in MM, is considered to be responsible for this instability. As endonucleases play an important role in mediating HR, here we have evaluated the role of endonuclease in biology and progression of MM. Gene expression profile using Affymetrix U133 array showed > 2 fold elevation of Ape1 or Ape2 or both in 5 of 6 MM cell lines and 12 of 15 patient samples. Immunocytochemistry confirmed upregulation of Ape1 protein in MM cell lines. A Plasmid degradation assay
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Landthaler, Markus, Nelson C. Lau, and David A. Shub. "Group I Intron Homing in Bacillus Phages SPO1 and SP82: a Gene Conversion Event Initiated by a Nicking Homing Endonuclease." Journal of Bacteriology 186, no. 13 (2004): 4307–14. http://dx.doi.org/10.1128/jb.186.13.4307-4314.2004.

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ABSTRACT Many group I introns encode endonucleases that promote intron homing by initiating a double-stranded break-mediated homologous recombination event. In this work we describe intron homing in Bacillus subtilis phages SPO1 and SP82. The introns encode the DNA endonucleases I-HmuI and I-HmuII, respectively, which belong to the H-N-H endonuclease family and possess nicking activity in vitro. Coinfections of B. subtilis with intron-minus and intron-plus phages indicate that I-HmuI and I-HmuII are required for homing of the SPO1 and SP82 introns, respectively. The homing process is a gene co
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GRISHIN, ALEXANDER, INES FONFARA, ANDREI ALEXEEVSKI, et al. "IDENTIFICATION OF CONSERVED FEATURES OF LAGLIDADG HOMING ENDONUCLEASES." Journal of Bioinformatics and Computational Biology 08, no. 03 (2010): 453–69. http://dx.doi.org/10.1142/s0219720010004665.

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LAGLIDADG family of homing endonucleases are rare-cutting enzymes which recognize long target sequences and are of great interest in genome engineering. Despite advances in homing endonuclease engineering, effective methods of broadening the range of cleaved sequences are still lacking. Here, we present a study of conserved structural features of LAGLIDADG homing endonucleases that might aid further development of such methods. The protein–DNA interface of LAGLIDADG homing endonucleases differs considerably with the particular nuclease, and the analysis of conserved protein–DNA interactions co
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Everett, Blake A., Lauren A. Litzau, Kassidy Tompkins, et al. "Crystal structure of the Wheat dwarf virus Rep domain." Acta Crystallographica Section F Structural Biology Communications 75, no. 12 (2019): 744–49. http://dx.doi.org/10.1107/s2053230x19015796.

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The Rep domain of Wheat dwarf virus (WDV Rep) is an HUH endonuclease involved in rolling-circle replication. HUH endonucleases coordinate a metal ion to enable the nicking of a specific ssDNA sequence and the subsequent formation of an intermediate phosphotyrosine bond. This covalent protein–ssDNA adduct makes HUH endonucleases attractive fusion tags (HUH-tags) in a diverse number of biotechnological applications. Solving the structure of an HUH endonuclease in complex with ssDNA will provide critical information about ssDNA recognition and sequence specificity, thus enabling rationally engine
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Bultmann, H., and R. Mezzanotte. "Characterization and origin of extrachromosomal DNA granules in Sarcophaga bullata." Journal of Cell Science 88, no. 3 (1987): 327–34. http://dx.doi.org/10.1242/jcs.88.3.327.

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We have used endonuclease treatment in situ, followed by Giemsa or ethidium bromide staining, for mapping repetitive sequences on the chromosomes of the flesh fly Sarcophaga bullata and thus for studying extrachromosomal DNA granules in this species. All three restriction enzymes employed (HaeIII, A1uI and HindIII) show the same cytological effects, except for a single interstitial band. In both polytene and mitotic chromosomes, chromatin resistant to these endonucleases presumably includes at least three endonucleases presumably includes at least three previously unrecognized buoyant density
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Jordano-Raya, Marina, Cristina Beltrán-Melero, M. Dolores Moreno-Recio, et al. "Complementary Functions of Plant AP Endonucleases and AP Lyases during DNA Repair of Abasic Sites Arising from C:G Base Pairs." International Journal of Molecular Sciences 22, no. 16 (2021): 8763. http://dx.doi.org/10.3390/ijms22168763.

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Abasic (apurinic/apyrimidinic, AP) sites are ubiquitous DNA lesions arising from spontaneous base loss and excision of damaged bases. They may be processed either by AP endonucleases or AP lyases, but the relative roles of these two classes of enzymes are not well understood. We hypothesized that endonucleases and lyases may be differentially influenced by the sequence surrounding the AP site and/or the identity of the orphan base. To test this idea, we analysed the activity of plant and human AP endonucleases and AP lyases on DNA substrates containing an abasic site opposite either G or C in
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Carnes, Jason, Carmen Zelaya Soares, Carey Wickham, and Kenneth Stuart. "Endonuclease Associations with Three Distinct Editosomes in Trypanosoma brucei." Journal of Biological Chemistry 286, no. 22 (2011): 19320–30. http://dx.doi.org/10.1074/jbc.m111.228965.

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Three distinct editosomes, typified by mutually exclusive KREN1, KREN2, or KREN3 endonucleases, are essential for mitochondrial RNA editing in Trypanosoma brucei. The three editosomes differ in substrate endoribonucleolytic cleavage specificity, which may reflect the vast number of editing sites that need insertion or deletion of uridine nucleotides (Us). Each editosome requires the single RNase III domain in each endonuclease for catalysis. Studies reported here show that the editing endonucleases do not form homodimeric domains, and may therefore function as intermolecular heterodimers, perh
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Dissertations / Theses on the topic "Endonucleasi"

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FIRRITO, CLAUDIA. "Targeted Gene Correction and Reprogramming of SCID-X1 Fibroblasts to Rescue IL2RG Expression in iPSC-derived Hematopoietic Cells." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2015. http://hdl.handle.net/10281/94656.

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La terapia genica basata sull’utilizzo di vettori integranti è stata già applicata con successo per la cura di varie malattie genetiche come le malattie da accumulo lisosomiale (LSD), la beta-talassemia (β-Thal) e le immunodeficienze primarie (PID). L’immunodeficienza combinata grave legata al cromosoma X (SCID-X1) è una malattia monogenica letale causata da mutazioni del gene codificante la catena comune gamma del recettore per l’interleuchina 2 (IL2RG). I primi studi clinici per la SCID-X1 hanno mostrato il potenziale terapeutico della terapia genica basata su vettori integranti, risultando
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Daniels, Lucy Elizabeth. "The SgrAI restriction endonuclease." Thesis, University of Bristol, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393877.

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Chevalier, Brett S. "Homing endonuclease mechanism, structure and design /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/4984.

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AlMalki, Faizah. "Structural studies on flap endonuclease complexes." Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/7293/.

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Flap endonucleases (FENs) are structure-specific enzymes that play critical roles in DNA replication and repair. Three members of the FEN family have been investigated during this project in complexes with DNA substrates and metal ions: bacteriophage T5FEN, hFEN and Trypanosoma brucei FEN. T5FEN wild type and two catalytically inactive versions, D153K and D155K were successfully crystallized in complexes with DNA substrates containing 5' or 3' overhangs. The crystal structure for T5FEN-D153K in complex with a duplex containing 5' overhangs at each end and two Mg2+ ions was solved. The structur
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Barzilay, Gil. "Characterisation of human AP endonuclease I (HAP1)." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.318791.

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Pernstich, Christian. "Protein dynamics of the restriction endonuclease Fokl." Thesis, University of Bristol, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.526007.

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Stanford, Neil Philip. "DNA cleavage by the EcoRV restriction endonuclease." Thesis, University of Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299311.

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Wentzell, Lois Marie. "DNA communications by the SfiI restriction endonuclease." Thesis, University of Bristol, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388002.

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Hanson, Mark Nils. "Biochemical characterization of the endonuclease PMR-1 /." The Ohio State University, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=osu1488204276532461.

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Zhao, Lei. "Characterization of bacterial homing endonuclease I-Ssp6803I /." Thesis, Connect to this title online; UW restricted, 2008. http://hdl.handle.net/1773/9214.

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Books on the topic "Endonucleasi"

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Edgell, David R., ed. Homing Endonucleases. Humana Press, 2014. http://dx.doi.org/10.1007/978-1-62703-968-0.

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Pingoud, Alfred M., ed. Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0.

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Belfort, Marlene, David W. Wood, Barry L. Stoddard, and Victoria Derbyshire, eds. Homing Endonucleases and Inteins. Springer Berlin Heidelberg, 2005. http://dx.doi.org/10.1007/3-540-29474-0.

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G, Chirikjian Jack, ed. Restriction endonucleases and methylases. Elsevier, 1987.

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Bolton, Bryan John. Class ii restriction endonucleases: Screening, purification and characterization. University of Salford, 1988.

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Cross, Stephen R. H. Restriction endonuclease map variation and natural selection in populations of Drosophila melanogaster. University of Birmingham, 1985.

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Fraser, Murray J. Endo-exonucleases. R.G. Landes Co., 1996.

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Price, Rebecca Clare. The effects of restriction endonucleases on mammalian cells of different radiosensitivity. University of Manchester, 1994.

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Krabbe, Margareta. In vivo studies of Escherichia coli: Bacteriophage T4 endonuclease II-dependent restriction and initiation of plasmid R1 replication. Acta Universitatis Upsaliensis, 1995.

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Cartwright, Nicola. Detection and typing of human papillomavirus using semi-nested PCR and restriction endonuclease analysis with respect to vulval carcinoma. typescript, 1996.

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Book chapters on the topic "Endonucleasi"

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Gooch, Jan W. "Endonuclease." In Encyclopedic Dictionary of Polymers. Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13647.

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Roberts, R. J., M. Belfort, T. Bestor, et al. "A Nomenclature for Restriction Enzymes, DNA Methyltransferases, Homing Endonucleases, and Their Genes." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_1.

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Reuter, M., M. Mücke, and D. H. Krüger. "Structure and Function of Type IIE Restriction Endonucleases — or: From a Plasmid That Restricts Phage Replication to A New Molecular DNA Recognition Mechanism." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_10.

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Welsh, A. J., S. E. Halford, and D. J. Scott. "Analysis of Type II Restriction Endonucleases that Interact with Two Recognition Sites." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_11.

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Sidorova, N., and D. C. Rau. "The Role of Water in the EcoRI-DNA Binding." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_12.

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Cowan, J. A. "Role of Metal Ions in Promoting DNA Binding and Cleavage by Restriction Endonucleases." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_13.

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Horton, J. R., R. M. Blumenthal, and X. Cheng. "Restriction Endonucleases: Structure of the Conserved Catalytic Core and the Role of Metal Ions in DNA Cleavage." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_14.

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Alves, J., and P. Vennekohl. "Protein Engineering of Restriction Enzymes." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_15.

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Kandavelou, K., M. Mani, S. Durai, and S. Chandrasegaran. "Engineering and Applications of Chimeric Nucleases." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_16.

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Kobayashi, I. "Restriction-Modification Systems as Minimal Forms of Life." In Restriction Endonucleases. Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18851-0_2.

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Conference papers on the topic "Endonucleasi"

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"Comparison of the conformational dynamics of structurally different AP-endonucleases APE1 and Nfo during AP-endonuclease activity." In Systems Biology and Bioinformatics (SBB-2021) : The 13th International Young Scientists School;. ICG SB RAS, 2021. http://dx.doi.org/10.18699/sbb-plantgen-2021-16.

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Martyushova, V. G., A. M. Timofeeva, and S. E. Sedykh. "METHYLATION-DEPENDENT RESTRICTION ENDONUCLEASES FOR THE ANALYSIS OF METHYLATION IN PROMOTER REGIONS OF GENES ASSOCIATED WITH THE PATHOGENESIS OF ALZHEIMER’S SYNDROME." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-255.

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DNA methylation plays an important role in epigenetic inheritance. Methylation of promoter regions of genes associated with the pathogenesis of Alzheimer’s disease has been shown to change during the development of the disease. In this work, a methylation-dependent restriction endonuclease and qPCR were used to analyse this process.
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Sakovina, L. V., A. V. Endutkin, and D. O. Zharkov. "INTERFEROMETRIC SCATTERING MICROSCOPY FOR INVESTIGATION SPYCAS9 ENDONUCLEASE AND GUIDE SGRNA COMPLEX." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-268.

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Using interferometric scattering microscopy (ISM), based on the principles of interference-reflection microscopy and interferometric scattering microscopy [1], it is possible to measure the molecular masses of individual molecules. In this study, we evaluated the ISM method to investigate the biochemical properties of the SpyCas9 endonuclease and SpyCas9 and guide sgRNA complex.
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Orlovskaya, P. I., T. A. Pilipchuk, N. I. Girilovich, M. N. Mandrik-Litvinkovich, and E. I. Kalamiyets. "Investigation of genetic heterogeneity of phages from phytopathogenic bacteria Xanthomonas phaseoli." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.188.

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Kitajima, Tsubasa, Akira Hirata, Chikako Iwashita, Shin-ichi Yokobori, and Hiroyuki Hori. "Enzymatic and crystallographic characterization of archaeal tRNA splicing endonuclease." In 2009 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2009. http://dx.doi.org/10.1109/mhs.2009.5352027.

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Topka, Sabine, Sara Kazzaz, Kenneth Offit, and Vijai Joseph. "Abstract 5367: Ngago: no evidence of targeted endonuclease activity." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-5367.

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Wang, Yuejun, Yihong Qiu, Zhende Huang, and Zhu Yisheng. "Modelling on the Kinetics Mechanism of the FokI Restriction Endonuclease." In 2007 1st International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2007. http://dx.doi.org/10.1109/icbbe.2007.6.

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"Generation of haploidy inducers for Cas endonuclease-mediated mutagenesis in barley." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-178.

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Mačková, Michaela, and Michal Hocek. "Vinyl-modified DNA and its cleavage by restriction endonucleases." In XVIth Symposium on Chemistry of Nucleic Acid Components. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2014. http://dx.doi.org/10.1135/css201414318.

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"Structural features of substrate recognition by APE1-like endonucleases." In Bioinformatics of Genome Regulation and Structure/Systems Biology (BGRS/SB-2022) :. Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 2022. http://dx.doi.org/10.18699/sbb-2022-570.

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Reports on the topic "Endonucleasi"

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Hansch, Heidi. Designing and Assessing the Efficacy of Protein Inhibitors of IscB Endonucleases. Montana State University, 2025. https://doi.org/10.15788/1751903932.

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Many bacteria and archaea possess Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) proteins, forming a CRISPR-Cas system that defends against viral infection. These microbes incorporate fragments of viral DNA as spacers between short DNA repeats, then transcribe these regions of alternating spacers and repeat units into guide RNA (gRNA) sequences that form complexes with Cas proteins. Upon subsequent viral attack, the gRNA sequences bind regions of complementary viral DNA, and the Cas proteins act as endonucleases, cleaving the DNA to curb the infe
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Yeung, Anthony T. Detection of Mutations Using a Novel Endonuclease. Defense Technical Information Center, 1998. http://dx.doi.org/10.21236/adb238444.

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Lue, Neal F. Structural and Functional Characterization of a Telomerase-Associated Endonuclease. Defense Technical Information Center, 2002. http://dx.doi.org/10.21236/ada411390.

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Lue, Neal F. Structural and Functional Characterization of a Telomerase-Associated Endonuclease. Defense Technical Information Center, 2003. http://dx.doi.org/10.21236/ada417028.

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Feigon, Juli. Recognition of DNA by EcoRI Restriction Endonuclease and Methylase. Defense Technical Information Center, 1992. http://dx.doi.org/10.21236/ada247626.

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Braun, W. A. Molecular Recognition of DNA Damage Sites by Apurinic/Apyrimidinic Endonucleases. Office of Scientific and Technical Information (OSTI), 2005. http://dx.doi.org/10.2172/877152.

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Witz, Alexander, Andrew Schaefer, Dan Blake, and Nic Preyat. A proposal to align release standards for endonucleases used in nucleic acid removal. BioPhorum, 2023. http://dx.doi.org/10.46220/2023cgt002.

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Knoche, K., S. Selman, and L. Hung. Site specific endonucleases for human genome mapping. Final report, April 1, 1992--March 31, 1994. Office of Scientific and Technical Information (OSTI), 1994. http://dx.doi.org/10.2172/188888.

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Wilson, Thomas E., Avraham A. Levy, and Tzvi Tzfira. Controlling Early Stages of DNA Repair for Gene-targeting Enhancement in Plants. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7697124.bard.

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Abstract:
Gene targeting (GT) is a much needed technology as a tool for plant research and for the precise engineering of crop species. Recent advances in this field have shown that the presence of a DNA double-strand break (DSB) in a genomic locus is critical for the integration of an exogenous DNA molecule introduced into this locus. This integration can occur via either non-homologous end joining (NHEJ) into the break or homologous recombination (HR) between the broken genomic DNA and the introduced vector. A bottleneck for DNA integration via HR is the machinery responsible for homology search and s
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