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Dissertations / Theses on the topic 'Endoxylanase'

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Published: 4 June 2021
Last updated: 26 July 2025

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1

Lewis, Elizabeth A. "Expression of a clostridium acetobutylicum endoglucanase and an endoxylanase in saccharomyces cerevisiae." Master's thesis, University of Cape Town, 1989. http://hdl.handle.net/11427/21899.

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Bibliography: pages 128-141.<br>S. cerevisiae is widely used in industrial processes, in particular ethanol production. The aim of this study was to examine the feasability of cloning lignocellulase genes into yeast. These enzymes could possibly extend the substrate range of S. cerevisiae, thereby possibly making the process of ethanol production more cost effective.
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2

Benamrouche-Stitou, Yamina. "Biochimie et histologie de la dégradation enzymatique du son de blé par une endoxylanase." Reims, 2002. http://www.theses.fr/2002REIMS011.

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Les co-produits agricoles représentent une matière première renouvelable disponible en quantité importante pouvant être valorisée par bioconversion enzymatique. La valorisation des substrats lignocellulosiques par voie enzymatique représente une alternative aux procédés chimiques polluants et non spécifiques, généralement employés. Le son de blé, co-produit issu de la production du blé, est particulièrement riche en hémicelluloses (40 % de sa matière sèche). L’hydrolyse du son de blé par une β (1,4) endoxylanase thermostable de 20 KDa permet la libération de 50 % des arabinoxylanes. Ce taux ma
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3

Silva, Mylenne Calciolari Pinheiro da. "Bioprospecção de xilanase e α-amilase por meio de métodos independentes e dependentes de cultivo microbiano em um solo de manguezal." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-08012016-102539/.

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A xilanase e a &alpha;-amilase são enzimas responsáveis por degradar parte da matéria orgânica nos solos, atuando na mineralização da hemicelulose e do amido, respectivamente. Do ponto de vista biotecnológico, estas enzimas, assim como os subprodutos gerados pela sua ação enzimática, podem ser utilizados em diversos setores industriais. Os manguezais podem representar uma fonte promissora e inexplorada para a busca de enzimas microbianas em função das altas quantidades de matéria vegetal em decomposição presente neste ecossistema, onde ocorrem condições ambientais únicas, representadas pela co
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4

Zappe, Harold. "The cloning and characterisation of an endoglucanase and an endoxylanase from Clostridium acetobutylicum in Escherichia coli." Doctoral thesis, University of Cape Town, 1988. http://hdl.handle.net/11427/21911.

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Bibliography: pages 215-244.<br>Clostridium acetobutylicum P262 is an endospore forming Gram-positive obligate anaerobe which has been used for the industrial production of acetone and butanol. Strains of C. acetobutylicum have been reported to exhibit some activity towards cellulosic and hemicellulosic substrates. The aim of this thesis was to establish a genebank of C. acetobutylicum P262 DNA in Escherichia coli and to isolate and characterise genes encoding enzymes which show activity towards hemicellulose and cellulose.
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5

Bataillon, Michel. "Isolement d'une souche thermophile xylanolytique et purification d'une endoxylanase. Valorisation des heteroxylanes du son de ble par hydrolyse enzymatique." Paris, Institut national d'agronomie de Paris Grignon, 1998. http://www.theses.fr/1998INAP0026.

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L'hydrolyse des arabinoxylanes du son de ble par des xylanases thermostables a ete etudiee. Les arabinoxylanes ont ete extraits du son de ble afin de pouvoir realiser une selection de micro-organismes hemicellulolytiques thermophiles. Une souche proche de bacillus flavothermus secretant des xylanases thermostables a ete ainsi isolee d'une source chaude du portugal. Apres amelioration des conditions de culture, les xylanases ont ete produites en fermenteurs puis purifiees jusqu'a homogeneite. L'enzyme purifiee a un poids moleculaire de 99 kda. L'optimum d'activite est de 75c. L'enzyme est activ
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6

Tahir, Tariq Ahmed. "Expression and mutagenesis studies of 1,4-β-D-xylanohydrolases : structural insights into the interaction with substrate and wheat endoxylanase inhibitors." Thesis, University of East Anglia, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273580.

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7

LEQUART, CHRISTELLE. "Destructuration des parois vegetales lignifiees par une endoxylanase et un complexe abiotique mno2/oxalate-application a la paille de ble." Reims, 1999. http://www.theses.fr/1999REIMS010.

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Les co-produits agricoles constituent une matiere premiere renouvelable et disponible en grande quantite. La valorisation des lignocelluloses par voie enzymatique represente une alternative aux procedes chimiques polluants employes pour leur fractionnement. Le son et la paille de ble ont ete hydrolyses par une endoxylanase purifiee pour la production d'oligosaccharides. Le taux de lignines, les interactions entre les lignines et les hemicelluloses par le biais des acides hydroycinnamiques ainsi que la nature des xylanes semblent influencer l'hydrolyse enzymatique. L'etude d'une fraction des pr
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8

Generoso, Wesley Cardoso. "Expressão recombinante e caracterização de uma endoxilanase não descrita de Trichoderma harzianum." Universidade Federal de São Carlos, 2012. https://repositorio.ufscar.br/handle/ufscar/5505.

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Made available in DSpace on 2016-06-02T20:21:29Z (GMT). No. of bitstreams: 1 4498.pdf: 2023528 bytes, checksum: b6d98480ef338423f24d2704afae90bd (MD5) Previous issue date: 2012-06-29<br>Universidade Federal de Minas Gerais<br>The use of alternative and renewable fuels is vital to solve the problems derived from the dependence of fuels, and the vegetal biomass is the only renewable source available in large scale that can be converted in energy. The bioethanol production has demonstrated a huge potential to minimize the environmental changes resultant from fossil fuels consumption. Neverthele
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9

Rémond, Caroline. "Etude des mecanismes de degradation enzymatique des parois vegetales. Application a l'hydrolyse de la paille de ble par une endoxylanase purifiee." Reims, 1996. http://www.theses.fr/1996REIMS031.

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Les sous-produits agricoles, tels que les pailles des plantes cerealieres, sont riches en polysaccharides et representent une ressource naturelle abondante. La valorisation de ces matieres lignocellulosiques par voie enzymatique offre non seulement l'avantage d'etre specifique, mais elle est aussi non polluante compare aux hydrolyses chimiques classiques. La paille de ble est riche en xylanes qui constituent 20-25% de sa matiere seche. L'hydrolyse totale de la paille de ble par des complexes enzymatiques peut conduire a la synthese de biocarburants. L'hydrolyse menagee de la paille par des xyl
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10

Lunkes, Jaina Caroline. "Purificação e caracterização bioquímica de xilanase-I produzida por Penicillium crustosum e sua aplicação." Universidade Estadual do Oeste do Parana, 2015. http://tede.unioeste.br:8080/tede/handle/tede/6.

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Made available in DSpace on 2017-05-12T14:36:23Z (GMT). No. of bitstreams: 1 dissetaccao_ jaina convertido encadernadora.pdf: 2928797 bytes, checksum: 85949e85bf169f2b4b0fd1a4a31a60bf (MD5) Previous issue date: 2015-12-08<br>The! xylanolytic! complex! is! formed! by! a! group! of! enzymes! capable! of! acting! on! multiple!binding!sites!in!the!chain!of!xylan,!hemicellulose!one!found!in!plant!cell!walls! and! to! degrade! it! into! Xylooligosaccharides,! xilotrioses,! xilobioses! and! xylose.! The! endoI1,4I&#946;Ixylanases! (1,4I&#946;IDIxylan!xylanohydrolasen!EC!3.2.1.8),!which!hydrolyze! t
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11

Ordaz, Ortiz José Juan. "Dégradation enzymatique des parois du grain de blé (Triticum aestivum) par une endoxylanase de Trichoderma viride : Application à l'étude de la structure des arabinoxylanes et de leur variabilité." Nantes, 2005. http://www.theses.fr/2005NANT2031.

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La dégradation enzymatique des parois du grain de blé (Triticum aestivum) et son application à l'étude de la variabilité structurale des arabinoxylanes (AX) de l'albumen amylacé a été entreprise pour mieux comprendre les propriétés des parois et maîtriser les mécanismes de biosynthèse qui les modulent. Les produits de dégradation des AX par une endoxylanase de Trichoderma viride ont été isolés et caractérisés par RMN du proton, puis utilisés pour produire des anticorps monoclonaux dirigés contre les AX. Les règles de leur fragmentation en spectrométrie de masse (ESI-Q-TOF) ont été établies. La
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12

Gracioli, Michel Ricardo [UNESP]. "Purificação e caracterização de uma xilanase halotolerante de Aspergillus hortai CRM 1919 e aplicação na hidrólise de subprodutos agroindustriais." Universidade Estadual Paulista (UNESP), 2018. http://hdl.handle.net/11449/153582.

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Submitted by Michel Ricardo Gracioli (michel-rg@hotmail.com) on 2018-04-17T15:51:19Z No. of bitstreams: 1 Dissertação Michel.pdf: 1706772 bytes, checksum: 1d66fae75b86a68d89ec8bceca0d2f31 (MD5)<br>Approved for entry into archive by Adriana Aparecida Puerta null (dripuerta@rc.unesp.br) on 2018-04-18T14:13:36Z (GMT) No. of bitstreams: 1 gracioli_mr_me_rcla.pdf: 1697470 bytes, checksum: 82696e96fe2dfed0fbd786c7230a8994 (MD5)<br>Made available in DSpace on 2018-04-18T14:13:36Z (GMT). No. of bitstreams: 1 gracioli_mr_me_rcla.pdf: 1697470 bytes, checksum: 82696e96fe2dfed0fbd786c7230a8994 (MD5)
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13

Roets, Carien. "Effect of endoxylanases, endoglucanases and their combination on wheat flour bread quality." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/2800.

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Thesis (Msc Food Sc (Food Science))--University of Stellenbosch, 2009.<br>Endoxylanases are known to improve dough stability, oven spring, loaf volume, crumb structure and shelf life. The use of endoglucanases (cellulases) usually results in increased bread loaf volume, bread score and reduced crumb firmness. Even though bakeries use ‘pure’ enzymes in their formulations, they are supplied with an enzyme mixture which can contain up to five different enzymes. These mixtures often also include an emulsifier and ascorbic acid. To compare the ability of endoxylanase and endoglucanase to impro
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14

Chang, Yung-Chieh, and 張雲傑. "Protein engineering of an endoxylanase from Trichoderma koningii G-39." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/47474223373229876354.

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15

Ottenheim, Christoph, Kirstin Meier, Wolfgang Zimmermann, and Jin Chuan Wu. "Isolation of filamentous fungi exhibiting high endoxylanase activity in lignocellulose hydrolysate." 2015. https://ul.qucosa.de/id/qucosa%3A16855.

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For complete degradation of hemicellulose into its monomers from lignocellulose biomass, the synergistic action of a broad range of hydrolytic enzymes is needed. Therefore, production of enzymes from their natural producer is desirable. To obtain a powerful b-1,4-endoxylanase producing fungus, 304 environmental samples were collected from various locations in Singapore, leading to 603 isolates. Among them, 71 exhibiting b-1,4-endoxylanase activity were identified belonging mainly to the genera of Aspergillus, Penicillium, and Trichoderma. Further analysis revealed Aspergillus niger DSM 26641 a
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16

Hsueh, Tsung-Han, and 薛琮翰. "Over-Expression of Endoxylanase from Alkaliphilic Bacillus halodurans in Recombinant Escherichia coli." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/753s7p.

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碩士<br>國立中正大學<br>化學工程研究所<br>102<br>Extracellular endo-1,4-β-xylanase encoded by a gene xyn45 from Bacillus halodurans was over-expressed in recombinant Escherichia coli BL21 (DE3) using plasmid pET29a as the vector. The influence of culture medium (LB or PM medium) and concentration of inducer (IPTG or lactose in different concentration levels) on the production of this extracellular endoxylanase was studied. Using low concentration of IPTG as the inducer, the total activity of endoxylanase was higher in PM medium than that in LB medium. As the cultivation was in PM medium, the use of IPTG led
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17

Sun, We-Len, and 孫文仁. "Synergistic effect of endoxylanase from Bacillus licheniformis and optimal production of xylooligosaccharides." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/73858193645185733173.

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碩士<br>國立屏東科技大學<br>食品科學系<br>94<br>The objective of the present study is to conduct the hydrolysate profiles of xylan, prebiotic effect of xylooligosaccharides and synergistic effect by a purified endoxylanase derived from Bacillus licheniformis. First all, a synergistic effect on birchwood xylan, oat-spelts xylan, corn cob, untreated sugar cane bagasse, H2SO4 treated corn cob and hemicellulose prepared from sugar cane bagasse by single xylanase, cellulase and xylosidase or combined enzymes among them were systematically established. Xylooligosaccharides prepared from birchwood and oat-spelts x
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18

Ottenheim, Christoph, Katharina A. Werner, Wolfgang Zimmermann, and Jin Chua Wu. "Improved endoxylanase production and colony morphology of Aspergillus niger DSM 26641 by g-ray induced mutagenesis." 2015. https://ul.qucosa.de/id/qucosa%3A16854.

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Aspergillus niger DSM 26641 was exposed to 60Co g-radiation to enhance the b-1,4-endoxylanase activity, restrict colony growth and improve robustness of pellets. The first promising mutant obtained after g-radiation of the fungal spores at 50-2000 Gy showed a restricted colony growth and an 82% enhancement in b-1,4-endoxylanase activity. The mutant was subjected to a second round of g-radiation at 1400 Gy generating a mutant with double the b-1,4-endoxylanase activity compared to the native strain. The selected final mutant, deposited as Aspergillus niger DSM 28712, showed a maximal saccharifi
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19

YOU, SHIOU-RONG, and 游琇絨. "Production of extracellular endoxylanase by high-cell-density culture of recombinant Escherichia coli and its application." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/35771919568370813166.

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20

Huang, Lina, та 黃麗娜. "Studies of an Endoxylanase and a Bi-functional β-Xylosidase╱ α-Arabinofuranosidase from Trichoderma koningii G-39". Thesis, 1994. http://ndltd.ncl.edu.tw/handle/97756467968923335929.

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21

Ottenheim, Christoph, Carl Verdejo, Wolfgang Zimmermann, and Jin Chuan Wu. "Hemicellulase production by Aspergillus niger DSM 26641 in hydrothermal palm oil empty fruit bunch hydrolysate and transcriptome analysis." 2014. https://ul.qucosa.de/id/qucosa%3A16853.

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Palm oil empty fruit bunches (EFB) is an abundant and cheap lignocellulose material in South East Asia. Its use as the sole medium for producing lignocellulose-hydrolyzing enzymes would increase its commercial value. A newly isolated Aspergillus niger DSM 26641 was investigated for its capability of producing hemicellulases in EFB hydrolysate obtained by treatment with pressurized hot water (1-20%, w/v) at 120-180◦C in a 1 L Parr reactor for 10-60 min. The optimal hydrolysate for the fungal growth and endoxylanase production was obtained when 10% (w/v) of empty fruit bunch was treated at 120◦C
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22

Zhang, Bo-Yao, and 張博堯. "Production of Endoxylanases by Recombinant Escherichia Coli for Generating Xylooligosaccharide from Pineapple Peel Residue." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/8s3r9e.

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碩士<br>國立中正大學<br>化學工程研究所<br>106<br>Alkaliphilic bacterium Bacillus halodurans Thonburi contains two endo-xylanase genes, xyn45 and xyn23. The DNA fragment xyn45-(G4S)2-xyn23, which was a fusion of these two endoxylanase genes connected by a linker peptide (G4S)2 sequence were cloned to pET29a(+) vector. E. coli BL21(DE3) was used as the host for the overexpression of recombinant fusion protein. The recombinant strain was called as E. coli BL21(DE3)-pET29a(+)-xyn45-(G4S)2-xyn23. The recombinant E. coli BL21(DE3)-pET29a(+)-xyn45 harboring xyn45 gene-containing plasmid was also studied for a compa
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