Relevant bibliographies by topics / Enterocytozoon hepatopenaei

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Academic literature on the topic 'Enterocytozoon hepatopenaei'

Author: Grafiati
Published: 4 June 2021
Last updated: 13 February 2022

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Journal articles on the topic "Enterocytozoon hepatopenaei"

1

Nkuba, Anord Charles, Gunanti Mahasri, Nunuk Dyah Retno Lastuti, and Adamu Ayubu Mwendolwa. "Correlation of Nitrite and Ammonia with Prevalence of Enterocytozoon hepatopenaei (EHP) in Shrimp (Litopenaeus vannamei) on Several Super-Intensive Ponds in East Java, Indonesia." Jurnal Ilmiah Perikanan dan Kelautan 13, no. 1 (March 31, 2021): 58. http://dx.doi.org/10.20473/jipk.v13i1.24430.

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AbstractEnterocytozoon hepatopenaei (EHP), a microsporidia parasite known to hinder shrimp growth by infecting its hepatopancreas, is recently an emerging infection for Litopenaeus vannamei farms in Indonesia. The present study was conducted to investigate the relationship between nitrite and ammonia concentration with EHP infection in the super-intensive ponds. Shrimps and water samples were collected from six ponds in aquaculture super-intensive ponds with a stocking density of 5,000 shrimps in each pond which is located in Pasuruan, Lamongan, and Tuban Regency, Indonesia. The water sample was taken to the laboratory for the measurement of ammonia and nitrite. The samples of ammonia and nitrite were detected by spectrophotometer, and the PCR was used to detect 18S rRNA of EHP. The PCR analysis that showed two positive samples of L. vannamei, from the ponds with a high concentration of ammonia and nitrite, were infected by EHP. The statistical analysis showed a significant correlation between ammonia and nitrite with the prevalence of EHP infections, where the Pearson correlation (r) was 0.980 and 0.943, respectively. There was a high prevalence of EHP infection with the increase in nitrite concentration and ammonia in pond four and pond six. The concentration of over 1mg/l of ammonia and nitrite could influence EHP infection prevalence in the shrimp farms. Highlight ResearchAmmonia and nitrite concentration influence the prevalence of Enterocytozoon hepatopenaei infection in Super intensive ponds.The estimated prevalence of Enterocytozoon hepatopenaei was 16.7%.PCR amplification analysis was used to detect the 18S ss-rRNA of Enterocytozoon hepatopenaei and 510-bp was obtained.
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2

Aranguren Caro, LF, HN Mai, O. Pichardo, R. Cruz-Flores, B. Hanggono, and AK Dhar. "Evidences supporting Enterocytozoon hepatopenaei association with white feces syndrome in farmed Penaeus vannamei in Venezuela and Indonesia." Diseases of Aquatic Organisms 141 (September 17, 2020): 71–78. http://dx.doi.org/10.3354/dao03522.

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White feces syndrome (WFS) is an emerging and poorly described disease characterized by the presence of floating white fecal strings in shrimp (Penaeus monodon and P. vannamei) grow-out ponds. WFS has been associated with several pathogens, including Enterocytozoon hepatopenaei. This association is based on the fact that in areas where E. hepatopenaei has been reported, there was also a high WFS prevalence. E. hepatopenaei is an emerging pathogen that has affected cultured shrimp in Indonesia, Vietnam, China, Thailand, and India. In 2016, we reported the presence of E. hepatopenaei in farmed P. vannamei in Venezuela. In this study, we describe the first case of WFS in Venezuela associated with E. hepatopenaei. The white fecal strings and shrimp displaying white feces along the gastrointestinal tract observed in this study were similar to the gross signs found in WFS-impacted P. vannamei in SE Asian countries. Furthermore, we describe a strong association between WFS and E. hepatopenaei in the samples obtained from Venezuela and Indonesia. Quantification of E. hepatopenaei in WFS-affected ponds, ponds with a history of WFS, and ponds with no WFS showed that E. hepatopenaei loads were significantly higher in WFS-affected ponds. Furthermore, these findings constitute the first report of WFS being associated with E. hepatopenaei in farmed shrimp in Latin America. Additionally, we propose that the gross signs of WFS such as floating whitish fecal strings can be used as an indicator of the presence of E. hepatopenaei in countries where E. hepatopenaei is endemic.
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3

Artanto, Yohanes Kristiawan, Slamet Budi Prayitno, Sarjito Sarjito, Desrina Desrina, and Alfabetian Harjuna Condro Haditomo. "Molecular Characteristics of Indonesian Isolate Enterocytozoon hepatopenaei Based on Sequence Analysis of 18S rRNA Genes." Omni-Akuatika 15, no. 1 (July 3, 2019): 93. http://dx.doi.org/10.20884/1.oa.2019.15.1.694.

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ABSTRACT Enterocytozoon hepatopenaei (EHP) infection have been reported as an obstacle of whiteleg shrimp (Penaeus vannamei) culture in Indonesia. However, understanding of the molecular characteristics of EHP species in Indonesia is not widely known. The aims of this study were to determine the identity and characters of DNA, and their phylogeny of EHP species from several different locations in Indonesia with specific references to 18S rRNA gene. The EHPs were collected from cultured P.vannamei from Lampung, Pangandaran, Sidoarjo, Banyuwangi, Probolinggo, Blitar, Makassar, and Lombok. Thirteen (13) samples were randomly selected to explore their gene characters through 18S rRNA gene sequencing. The primers used were EHP_F and EHP_1R. Parameter observed were DNA sequencing, nucleotide sequence homology with related available genes in the Gen Bank database, multiple sequence alignment, and reconstruction of genetic relationship trees. DNA sequence homology analysis showed that all samples had 99.89-100% similarity to Indian Enterocytozoon hepatopenaei (Accession Number MH259890.1 and MH260592.1). The alignment results illustrated that all EHP sequences of Indonesian isolates were 100% identical each other. The phylogenetic tree topology provided information that all sample accessions were in the same clade and spread evenly. The conclusion were that the Indonesian EHP species were identical (100%) and it could be said that they were genetically homogeneous. Keywords: Enterocytozoon hepatopenaei, 18S rRNA gene, Indonesia.
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Krishnan, Areekkal Navaneeth, Sudalayandi Kannappan, Panakkool Thamban Aneesh, Peter Ezhil Praveena, and Karingalakkandy Poochirian Jithendran. "Polychaete worm - A passive carrier for Enterocytozoon hepatopenaei in shrimp." Aquaculture 545 (December 2021): 737187. http://dx.doi.org/10.1016/j.aquaculture.2021.737187.

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5

Shen, Hui, Yi Qiao, Xihe Wan, Ge Jiang, Xianping Fan, Hui Li, Wenjun Shi, Libao Wang, and Xiaoran Zhen. "Prevalence of shrimp microsporidian parasite Enterocytozoon hepatopenaei in Jiangsu Province, China." Aquaculture International 27, no. 3 (March 22, 2019): 675–83. http://dx.doi.org/10.1007/s10499-019-00358-6.

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6

Karthikeyan, K., and R. Sudhakaran. "Shrimp Microsporidian Parasite Enterocytozoon Hepatopenaei (EHP):Threat for Cultivated Penaeid Shrimp." South Indian Journal of Biological Sciences 2, no. 4 (October 1, 2016): 388. http://dx.doi.org/10.22205/sijbs/2016/v2/i4/103444.

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7

Aldama-Cano, Diva January, Piyachat Sanguanrut, Natthinee Munkongwongsiri, José Cuauhtémoc Ibarra-Gámez, Ornchuma Itsathitphaisarn, Rapeepun Vanichviriyakit, Timothy W. Flegel, Kallaya Sritunyalucksana, and Siripong Thitamadee. "Bioassay for spore polar tube extrusion of shrimp Enterocytozoon hepatopenaei (EHP)." Aquaculture 490 (March 2018): 156–61. http://dx.doi.org/10.1016/j.aquaculture.2018.02.039.

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8

Cai, Shao-Xin, Fan-De Kong, Shu-Fei Xu, and Cui-Luan Yao. "Real-time loop-mediated isothermal amplification for rapid detection of Enterocytozoon hepatopenaei." PeerJ 6 (December 4, 2018): e5993. http://dx.doi.org/10.7717/peerj.5993.

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Background Enterocytozoon hepatopenaei (EHP) is a newly emerged microsporidian parasite that causes retarded shrimp growth in many countries. But there are no effective approaches to control this disease to date. The EHP could be an immune risk factor for increased dissemination of other diseases. Further, EHP infection involves the absence of obvious clinical signs and it is difficult to identify the pathogen through visual examination, increasing the risk of disease dissemination. It is urgent and necessary to develop a specific, rapid and sensitive EHP-infected shrimp diagnostic method to detect this parasite. In the present study, we developed and evaluated a rapid real-time loop-mediated isothermal amplification (real-time LAMP) for detection of EHP. Methods A rapid and efficient real-time LAMP method for the detection of EHP has been developed. Newly emerged EHP pathogens in China were collected and used as the sample, and three sets of specificity and sensitivity primers were designed. Three other aquatic pathogens were used as templates to test the specificity of the real-time LAMP assay. Also, we compared the real-time LAMP with the conventional LAMP by the serial dilutions of EHP DNA and their amplification curves. Application of real-time LAMP was carried out with clinical samples. Results Positive products were amplified only from EHP, but not from other tested species, EHP was detected from the clinical samples, suggesting a high specificity of this method. The final results of this assay were available within less than 45 min, and the initial amplification curve was observed at about 6 min. We found that the amplification with an exponential of sixfold dilutions of EHP DNA demonstrated a specific positive signal by the real-time LAMP, but not for the LAMP amplicons from the visual inspection. The real-time LAMP amplification curves demonstrated a higher slope than the conventional LAMP. Discussion In this study, pathogen virulence impacts have been increased in aquaculture and continuous observation was predominantly focused on EHP. The present study confirmed that the real-time LAMP assay is a promising and convenient method for the rapid identification of EHP in less time and cost. Its application greatly aids in the detection, surveillance, and prevention of EHP.
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Kesavan, Karthikeyan, Ravi Mani, Itami Toshiaki, and Raja Sudhakaran. "Quick report on prevalence of shrimp microsporidian parasite Enterocytozoon hepatopenaei in India." Aquaculture Research 48, no. 7 (April 20, 2016): 3980–84. http://dx.doi.org/10.1111/are.13078.

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10

Liu, Ya-Mei, Liang Qiu, An-Zhi Sheng, Xiao-Yuan Wan, Dong-Yuan Cheng, and Jie Huang. "Quantitative detection method of Enterocytozoon hepatopenaei using TaqMan probe real-time PCR." Journal of Invertebrate Pathology 151 (January 2018): 191–96. http://dx.doi.org/10.1016/j.jip.2017.12.006.

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Dissertations / Theses on the topic "Enterocytozoon hepatopenaei"

1

Tang, Kathy F. J., Jee Eun Han, Luis Fernando Aranguren, Brenda White-Noble, Margeaux M. Schmidt, Patharapol Piamsomboon, Eris Risdiana, and Bambang Hanggono. "Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp." ACADEMIC PRESS INC ELSEVIER SCIENCE, 2016. http://hdl.handle.net/10150/621443.

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White feces syndrome (WFS) is an emerging problem for penaeid shrimp farming industries in SE Asia countries, Thailand, Malaysia, Vietnam, Indonesia, China, and in India. This occurrence of this syndrome is usually first evidenced by the appearance of white fecal strings floating on surface of the shrimp ponds. The gross signs of affected shrimp include the appearance of a whitish hindgut and loose carapace, and it is associated with reduced feeding and growth retardation. To investigate the nature of the white feces syndrome, samples of white feces and shrimp hepatopancreas tissue were collected from Penaeus vannamei in affected farms in Indonesia, and these were examined histologically. Within the white feces, we found densely packed spores of the microsporidian Enterocytozoon hepatopenaei (abbreviated as EHP) and relatively fewer numbers of rod-shaped bacteria. From WFS ponds, hepatopancreas samples form 30 individual shrimp were analyzed by histology and in situ hybridization. The results showed that all of the shrimp examined were infected with EHP accompanied by septic hepatopancreatic necrosis (SHPN). Midgut epithelial cells were also infected and this increased the number of tissue types being affected by EHP. By PCR, EHP was detected in all the samples analyzed from WFS-affected ponds, but not in those sampled from healthy shrimp ponds. To determine the modes of transmission for this parasite, we performed feeding and cohabitation bioassays, the results showed that EHP can be transmitted through per os feeding of EHP-infected hepatopancreas tissue to healthy shrimp and through cohabitation ofinfected and healthy shrimp. In addition, we found the use of Fumagillin-B, an antimicrobial agent, was ineffective in either reducing or eliminating EHP in infected shrimp.
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Wiredu, Boakye Dominic. "Life in the nucleus : the genomic basis of energy exploitation by intranuclear Microsporidia." Thesis, University of Exeter, 2016. http://hdl.handle.net/10871/26108.

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The Microsporidia are obligate intracellular parasites that have jettisoned oxidation phosphorylative capabilities during their early evolutionary history and so rely on ATP import from their host and glycolysis for their energy needs. Some species form tight associations with the host’s mitochondria and this is thought to facilitate ATP sequestration by the developing intracellular microsporidian. The human parasite, Enterocytozoon bieneusi has however lost glycolytic capabilities and may rely entirely on ATP import from its host for energy. E. bieneusi belongs to the Enterocytozoonidae microsporidian family and recent rDNA-based phylogenetic studies have suggested it has close evolutionary ties with Enterospora canceri, a crab-infecting intranuclear parasite. Such a close evolutionary relationship implied that glycolysis might also be absent in the intranuclear parasite raising questions as to how this parasite obtains energy from its unusual niche that is physically walled off from the host mitochondria, the main source of ATP in the host cell. In this study, draft genomes of four species of the Enterocytozoonidae namely, Ent. canceri, E. hepatopenaei, Hepatospora eriocheir and Hepatospora eriocheir canceri and one non-Enterocytozoonidae species, Thelohania sp. were assembled and annotated (The genome assembly of Hepatospora eriocheir was provided by Dr. Bryony Williams). Phylogenomics performed with this and publicly available genomic data confirmed the close evolutionary ties between Ent. canceri and E. bieneusi. Comparative genomic analyses also revealed that glycolysis is indeed lost in all members of the Enterocytozoonidae family sequenced in this study, hinting to the relaxation of evolutionary pressures to maintain this pathway at the base of this microsporidian family. Despite this absence, the hexokinase gene was retained in all aglycolytic genomes analysed, and that of Ent. canceri was fused to a PTPA gene. Functional assays and yeast complementation assays suggest that this chimera is able to recognise glucose as a substrate but the heterologously expressed homolog of H. eriocheir cannot. Finally, phylogenomics have been used here to demonstrate that despite the morphological differences between three Hepatospora-like organisms parasitizing different crab hosts, they are the same species. This finding adds more weight to current evidence suggesting that morphology is not an ideal marker for taxonomical classification in the Microsporidia.
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3

LIU, YU-HSUAN, and 劉于瑄. "Establishment of Diagnostic Methods to Detect Enterocytozoon Hepatopenaei (EHP) Infection in Culture Litopenaeus Vannamei in Taiwan." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/773fgt.

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碩士
國立高雄大學
生命科學系碩士班
107
This study investigated the epizootic of Enterocytozoon hepatopenaei (EHP) infection from Litopenaeus vannamei cultured in Taiwan. In addition, the diagnostic methods for EHP infection were established using biotechnology. The diseased L. vannamei were collected in southern Taiwan, mainly in Pingtung, Kaohsiung and Tainan. The PCR and nested PCR were carried out with the specific primers (ENF779/ENR779 and ENF176/ENR176) in order to confirm the EHP infection. Furthermore, the tissues tropism and histopathological changes of EHP infection were evaluated by traditional histological method. The amplicon of EHP SSU rDNA partial gene fragments (779 bp) by PCR reaction were cloned, sequencing, and sequence alignment and phylogenetic analysis. The DIG-probe of EHP SSU rDNA partial gene fragments were prepared by PCR method. The dot blot hybridization and in situ hybridization detective methods for EHP were developed. The results showed EHP microsporidia were detected in L. vannamei in Tainan using PCR and Nested PCR. In Pingtung and Kaohsiung, EHP infection were confirmed by nested PCR. Histopathological study, the epithelial cells of hepatopancreas of L. vannamei were target tissue infected by EHP using H& E staining. Except for hepatopancreas, EHP infections were not observed in other organs in the diseased shrimp. The EHP SSU rDNA partial gene fragment of EHP isolated from Taiwan were found more than 99% nucleotide sequence similarities with isolates of Thailand, Vietnam, India, and China. The phylogenetic tree analysis, Taiwan isolate should be same as EHP isolated from L. vannamei cultured in Southeast Asia. The sensitivity of DIG-probe for EHP was 13 pg/ul using dot blot hybridization. By Dot blot hybridization, the EHP infection was detected in the diseased shrimp using the DNA extracted from EHP infected L. vannamei and confirmed it is suffered from EHP microsporidian. In situ hybridization test also successfully detected the EHP infection of the hepatopancreas of L. vannamei by DIG-probe. The sensitivity was excellent than that of traditional histopathology. Conclusion, the Enterocytozoon hepatopenaei (EHP) microsporidian infection was confirmed in the L. vannamei cultured in Taiwan using PCR, nested PCR, histopathology, dot blot hybridization, in situ hybridization methods. In future, the preventive and treatment methods for EHP should be further developed in Taiwan.
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