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Dissertations / Theses on the topic 'Enterohemorrhagic Escherichia coli'

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Published: 4 June 2021

Last updated: 7 July 2024

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1

Dadgar, Ashraf. "Detection of enterohemorrhagic Escherichia coli (EHEC)." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6011.

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<p>Escherichia coli is a natural inhabitant of the intestines of both humans and animals, but there are also several pathogenic types of E. coli which cause disease in humans.</p><p>Strains of enterohemorrhagic E. coli (EHEC) have been associated with outbreaks of diarrhea, hemorrhagic colitis and hemolytic uremic syndrome in humans. Most clinical signs of disease arise as a consequence of the production of shigatoxin 1 and 2 or combination of these toxins. Other major virulence factors include EHEC hemolysin and intimin, the product of the eae gene that is involved in attaching and effacing a

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Yu, Angel Chia-yu. "Structural analysis of an enterohemorrhagic Escherichia coli metalloprotease effector." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/42821.

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Mucins are proteins that contain dense clusters of α-O-GalNAc-linked carbohydrate chains and are the major component of the mucosal barrier that lines the mammalian gastrointestinal tract from mouth to gut. A critical biological function of mucins is to protect the underlying epithelial cells from infection. Enterohemorrhagic Escherichia. coli O157:H7 (EHEC), a bacterial pathogen that causes severe food and water borne disease, is capable of breaching this barrier and adhering to intestinal epithelial cells during infection. StcE (secreted protease of C1-esterase inhibitor) is a ~100 kDa zinc

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3

Treptow, Andrea Lauren. "Investigation of a Thermoregulated Gene in Pathogen Enterohemorrhagic Escherichia coli." Thesis, The University of Arizona, 2014. http://hdl.handle.net/10150/323222.

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4

MacDonald, Leslie Anne. "Antigenic relationship of enterohemorrhagic Escherichia coli hemolysin to other RTX toxins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ56344.pdf.

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Thomassin, Jenny-Lee. "Antimicrobial peptide resistance mechanisms used by Enteropathogenic and Enterohemorrhagic «Escherichia coli»." Thesis, McGill University, 2014. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=121462.

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Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) are Gram-negative pathogens that cause diarrheal disease in the developed and developing world. To cause infection, these pathogens must overcome innate host defenses, such as secreted cationic antimicrobial peptides (AMPs). There are two groups of human AMPs: cathelicidins (LL-37) and defensins (α-defensin 5). AMPs are expressed in specific locations of the human body. In the small intestine, the infectious niche for EPEC, human α-defensins 5 and 6 (HD-5 and HD-6) are abundant and there are low levels of LL-37. Conversely

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Morgan, Jason Kyle. "Genetic basis for the virulence of enterohemorrhagic Escherichia coli strain TW14359." Scholar Commons, 2014. https://scholarcommons.usf.edu/etd/5277.

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Enterohemorrhagic Escherichia coli (EHEC) is a virulent pathotype of E. coli that is associated with major outbreaks of hemorrhagic colitis and the life-threatening kidney disease hemolytic uremic syndrome. For successful host colonization and attachment to the intestinal mucosa, EHEC requires the locus of enterocyte effacement (LEE) pathogenicity island, which encodes a type III secretion system (TTSS) responsible for secreting and translocating effector proteins into host colonocytes. Regulation of the LEE is primarily directed through the first operon, LEE1, encoding the locus encoded regul

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7

Tsai, Wan-Ling. "Investigation of Systems For Detection of Enterohemorrhagic Escherichia Coli Contamination In Foods /." The Ohio State University, 1996. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487933245536054.

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8

Eichhorn, Inga [Verfasser]. "Microevolution of epidemiological highly relevant non-O157 enterohemorrhagic Escherichia coli (EHEC) / Inga Eichhorn." Berlin : Freie Universität Berlin, 2016. http://d-nb.info/1115722530/34.

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9

Chong, Yuwen. "Intimate interactions between enteropathogenic and enterohemorrhagic Escherichia coli and intestinal epithelium in vitro." Thesis, University College London (University of London), 2006. http://discovery.ucl.ac.uk/1445194/.

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Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are diarrhoeagenic human pathogens that colonize intestinal epithelial cells via an attaching and effacing (A/E) lesion. Intimate adherence is mediated through binding of the intimin adhesin to the bacterial translocated intimin receptor, Tir. EPEC adheres to all regions of the human intestine in the in vitro organ culture (IVOC) system. In contrast, although EHEC is associated with colonic pathology in human infections, a prototypical strain of EHEC has shown a restricted tropism towards follicle-associated epithel

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10

Lai, YuShuan (Cindy). "EspFU, an Enterohemorrhagic E. Coli Secreted Effector, Hijacks Mammalian Actin Assembly Proteins by Molecular Mimicry and Repetition: A Dissertation." eScholarship@UMMS, 2014. https://escholarship.umassmed.edu/gsbs_diss/715.

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Enterohemorrhagic E. coli (EHEC) is a major cause of food borne diarrheal illness worldwide. While disease symptoms are usually self-resolving and limited to severe gastroenteritis with bloody diarrhea, EHEC infection can lead to a life threatening complication known as Hemolytic Uremic Syndrome (HUS), which strikes children disproportionately and is the leading cause of kidney failure in children. Upon infection of gut epithelia, EHEC produces characteristic lesions called actin pedestals. These striking formations involve dramatic rearrangement of host cytoskeletal proteins. EHEC hijacks mam

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11

Lai, YuShuan (Cindy). "EspFU, an Enterohemorrhagic E. Coli Secreted Effector, Hijacks Mammalian Actin Assembly Proteins by Molecular Mimicry and Repetition: A Dissertation." eScholarship@UMMS, 2004. http://escholarship.umassmed.edu/gsbs_diss/715.

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Enterohemorrhagic E. coli (EHEC) is a major cause of food borne diarrheal illness worldwide. While disease symptoms are usually self-resolving and limited to severe gastroenteritis with bloody diarrhea, EHEC infection can lead to a life threatening complication known as Hemolytic Uremic Syndrome (HUS), which strikes children disproportionately and is the leading cause of kidney failure in children. Upon infection of gut epithelia, EHEC produces characteristic lesions called actin pedestals. These striking formations involve dramatic rearrangement of host cytoskeletal proteins. EHEC hijacks mam

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12

Willford, John Daniel. "Development of a field-based assay for rapid detection of enterohemorrhagic Escherichia coli (EHEC)." Laramie, Wyo. : University of Wyoming, 2008. http://proquest.umi.com/pqdweb?did=1663059651&sid=1&Fmt=2&clientId=18949&RQT=309&VName=PQD.

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13

Brady, Michael John. "Mechanism and Function of Actin Pedestal Formation by Enterohemorrhagic Escherichia coli O157:H7: A Dissertation." eScholarship@UMMS, 2007. https://escholarship.umassmed.edu/gsbs_diss/342.

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Enterohemorrhagic Escherichia coli O157:H7 (EHEC) and enteropathogenic E. coli O127:H7 (EPEC) induce characteristic F-actin rich pedestals on infected mammalian cells. Each pathogen delivers its own translocated intimin receptor (Tir) to the host cell to act as a receptor for the bacterial outer membrane adhesin, intimin. Interaction of translocated Tir with intimin is essential for mammalian cell binding and host colonization, as well as to induce actin pedestal formation in vitro. In spite of these parallels, EHEC and EPEC Tir appear to generate actin pedestals by distinct mechanisms. Furthe

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Liu, Hui. "Intimin-Tir Interaction in Enterohemorrhagic E. coli: A Dissertation." eScholarship@UMMS, 2000. http://escholarship.umassmed.edu/gsbs_diss/266.

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Enterohemorrhagic E. coli (EHEC) has emerged as an important agent of diarrheal disease in the developed countries. Attachment to host cells, an essential step during intestinal colonization by EHEC, is associated with the formation of a highly organized cytoskeletal structure containing filamentous actin, termed attaching and effacing (A/E) lesion, directly beneath bound bacteria. The outer membrane protein, intimin, is required for the formation of this structure, as is Tir, a bacterial protein that is translocated into the host cell and thought to function as a receptor for intimin. In this

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15

Yang, Yang. "Investigation of enterotoxigenic Escherichia coli (ETEC) vaccine candidates and identification of inhibitor of enterohemorrhagic Escherichia coli (EHEC) Type III secretion system effector NleB." Thesis, Kansas State University, 2017. http://hdl.handle.net/2097/38174.

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Master of Science in Biomedical Sciences<br>Department of Diagnostic Medicine/Pathobiology<br>Philip R. Hardwidge<br>Enterotoxigenic Escherichia coli (ETEC) is the most common cause of diarrhea in travellers and young children in developing countries. We previously characterized three vaccine candidates (MipA, Skp, and ETEC_2479) which effectively protected mice in an intranasal ETEC challenge model after immunization. However, these proteins are conserved not only in multiple ETEC isolates, but also in commensal bacteria. In this study, we examined the potential of these antigens to affect th

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Eißenberger, Kristina [Verfasser], and Herbert [Akademischer Betreuer] Schmidt. "Uptake of enterohemorrhagic Escherichia coli into the roots of lettuce plants / Kristina Eißenberger ; Betreuer: Herbert Schmidt." Hohenheim : Kommunikations-, Informations- und Medienzentrum der Universität Hohenheim, 2020. http://d-nb.info/1214296629/34.

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17

Quintanilla, Lucy Beatriz Zapata. "Anticorpos séricos anti Escherichia coli enterohemorrágica (EHEC) em adultos saudáveis da Grande São Paulo." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-19092006-134239/.

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As gastroenterites ainda são um importante problema de saúde pública nos países em desenvolvimento e as Escherichia colí são as principais causas de diarréias. Adultos brasileiros apresentam anticorpos reativos com E. colí enteropatogênica (EPEC), que apresenta muitas semelhanças com E. colí enterohemorágica (EHEC) e pode ser responsável por complicações como a síndrome hemolítica urêmica e a colite hemorrágica. Ambas as bactérias apresentam um mecanismo patogênico comum: a formação da lesão \"attaching and effacing\" nos microvilus dos enterócitos, mediadas por fatores de virulência, como Int

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18

Gardette, Marion. "Virulence des Escherichia coli entérohémmoragiques : rôle central du monoxyde d'azote dans le devenir de l'infection et identification de nouveaux déterminants impliqués dans l'adaptation du pathogène à l'envirronement digestif." Thesis, Université Clermont Auvergne‎ (2017-2020), 2019. http://www.theses.fr/2019CLFAC075.

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Les Escherichia coli entérohémorragiques (EHEC) représentent un enjeu majeur en santé publique. En effet, ces pathogènes sont responsables chaque année de milliers de cas de toxi-infections alimentaires à travers le monde et peuvent engendrer des complications graves, notamment des atteintes rénales chez les jeunes enfants et cérébrales chez les personnes âgées. Actuellement, le principal problème réside dans le fait que les traitements thérapeutiques disponibles sont limités puisque l’antibiothérapie peut favoriser le développement des complications liées à l’infection. Il est donc primordial

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Radhakrishnan, Padhma. "Role of Intimin and Tir in Actin Signalling by Enterohemorrhagic and Enteropathogenic Escherichia coli: A Dissertation." eScholarship@UMMS, 2003. http://escholarship.umassmed.edu/gsbs_diss/265.

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Enterohemorrhagic Escherichia coli 0157:H7 (EHEC) and Enteropathogenic E. coli (EPEC) are intestinal pathogens that induce characteristic lesions on mammalian cells called actin pedestals. Attachment to host cells by both EPEC and EHEC is an essential step towards colonization and is associated with the formation of highly organized actin cytoskeletal elements termed as attaching and effacing (AE) lesions beneath bound bacteria. The outer membrane protein intimin is required for the formation of these structures and binds its own translocated mammalian cell receptor called Translocated intimin

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20

Skehan, Brian M. "Functional Elements of EspF_u, an Enterohemorrhagic E. coli Effector that Stimulates Actin Assembly: A Dissertation." eScholarship@UMMS, 2009. https://escholarship.umassmed.edu/gsbs_diss/443.

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Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is an attaching and effacing pathogen that upon attachment to host cells, induce characteristic attaching and effacing lesions and formation of F-actin rich pedestals beneath sites of bacterial attachment. EHEC harbors a Type III secretion system through which it delivers dozens of effectors into the host cell. The two secreted effectors critical for EHEC-mediated actin pedestal formation are the translocated intimin receptor (Tir) and EspFU. EspFU consists of an N-terminal secretion signal and a C-terminus containing six tandem 47-residue prol

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21

Pongsri, Tongtawe Wanpen Chaicumpa. "Purification of shiga-like toxins (verocytotoxins) from enterohemorrhagic escherichia coli and the production of specific monoclonal antibodies to the toxins /." Abstract, 1999. http://mulinet3.li.mahidol.ac.th/thesis/2542/42E-PongsriT.pdf.

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22

Mallick, Emily M. "A New Murine Model For Enterohemorrhagic Escherichia coli Infection Reveals That Actin Pedestal Formation Facilitates Mucosal Colonization and Lethal Disease: A Dissertation." eScholarship@UMMS, 2012. https://escholarship.umassmed.edu/gsbs_diss/601.

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Enterohemorrhagic Escherichia coli (EHEC) colonizes the intestine and produces the phage-encoded Shiga toxin (Stx) which is absorbed systemically and can lead to hemolytic uremic syndrome (HUS) characterized by hemolytic anemia, thrombocytopenia, and renal failure. EHEC, and two related pathogens, Enteropathogenic E. coli (EPEC), and the murine pathogen, Citrobacter rodentium, are attaching and effacing (AE) pathogens that intimately adhere to enterocytes and form actin “pedestals” beneath bound bacteria. The actin pedestal, because it is a unique characteristic of AE pathogens, has been the s

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23

Stigers, Linnea. "Evaluation of Escherichia coli probiotic candidates for combating EHEC in the food chain using competition analysis in bovine feces." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-353248.

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Enterohemorrhagic E. coli, EHEC, is a verotoxin producing, zoonotic pathogen, which causes diseases in humans such as bloody or watery diarrhea. Microorganisms compete for limited living space, nutrients and other resources and therefore other microorganisms are EHECs biggest competitors. To avoid outbreaks and infections with EHEC, one possible approach is to use harmless but competitive bacteria as probiotics. Therefore, the aim of this study was to evaluate three probiotic E. coli strains and their ability to outcompete EHEC in bovine feces. Ten different cattle fecal samples from three

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Jaudou, Sandra. "Metadetect : detection of Shiga toxin-producing Escherichia coli with novel metagenomics approaches and its application on dairy farms in France and Germany." Electronic Thesis or Diss., Maisons-Alfort, École nationale vétérinaire d'Alfort, 2023. http://www.theses.fr/2023ENVA0004.

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Les méthodologies actuelles de caractérisation d'Escherichia coli producteur de toxine Shiga (STEC) nécessitent l'isolement de la souche, ce qui est compliqué par le fait qu'il n'existe pas de milieu d'isolement spécifique qui distingue clairement les STEC des E. coli commensaux non pathogènes. Par conséquent, obtenir des informations sur les souches en utilisant une approche métagénomique éviterait d'isoler les souches pour les caractériser complètement. Dans le cadre du projet, en collaboration avec le BfR en Allemagne, nous évaluerons si de nouvelles approches de métagénomique à lecture lon

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Rump, Lydia Vanessa Verfasser], and Markus [Akademischer Betreuer] [Fischer. "Molecular Characterization of Enterohemorrhagic Escherichia coli (EHEC) : O rough strains and the prevalence and importance of IS629 in E. coli O157:H7 / Lydia Vanessa Rump. Betreuer: Markus Fischer." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2011. http://d-nb.info/1020457465/34.

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Chagnot, Caroline. "Colonisation de la viande par Escherichia coli O157∶H7 : caractérisation moléculaire, cellulaire et tissulaire des interactions." Thesis, Clermont-Ferrand 2, 2014. http://www.theses.fr/2014CLF22448/document.

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Escherichia coli O157:H7 est le sérotype le plus souvent incriminé lors de toxi-infection alimentaire par les E. coli entérohémorragiques (EHEC). Il peut être associé, dans les cas les plus graves, à des colites hémorragiques mortelles et au syndrome hémolytique et urémique (SHU), touchant essentiellement les jeunes enfants. Le vecteur alimentaire le plus courant lors de ces contaminations est le boeuf haché. L’étape primaire de la contamination bactérienne se situe lors de l'abattage où les bactéries peuvent être transférées de la peau à la carcasse. Une gaine conjonctive entoure les muscles,

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Cordonnier, Charlotte. "Survie et pathogénicité des EHEC dans l'environnement digestif : Interactions avec le microbiote et l'épithélium intestinal. : Influence de l'administration de levures probiotiques." Thesis, Clermont-Ferrand 1, 2015. http://www.theses.fr/2015CLF1MM21/document.

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Les Escherichia coli entérohémorragiques (EHEC) sont des pathogènes majeurs pour l’homme responsables de toxi-infections alimentaires pouvant évoluer vers des complications potentiellement mortelles. La pathogénicité de ces souches est essentiellement due à la production de Shiga-toxines (Stx), même si d’autres facteurs semblent jouer un rôle important dans la virulence, comme des facteurs d’adhésion. La survie et la régulation des facteurs de virulence des EHEC dans l’environnement digestif humain sont des facteurs clés dans la pathogénicité bactérienne, mais restent à ce jour mal décrits, es

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Bansal, Tarun. "Inter-Kingdom Signaling Interactions in Enterohemorrhagic Escherichia coli Infections." Thesis, 2010. http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8177.

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The overall goal of this research was to understand the role of inter-kingdom signaling in enterohemorrhagic Escherichia coli (EHEC) infections of the human gastro-intestinal (GI) tract from the perspective of both the invading pathogen and the human intestinal epithelial cells, which they colonize. Differential gene expression of EHEC was studied upon exposure to the human neuroendocrine hormones epinephrine and norepinephrine. We determined that these hormones increase EHEC chemotaxis, motility, biofilm formation, colonization of host cells, and virulence gene expression. We also studied the

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Junkins, Alan Dean. "Studies on adherence of enterohemorrhagic Escherichia coli O157:H7." 1991. http://catalog.hathitrust.org/api/volumes/oclc/24262409.html.

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Thesis (Ph. D.)--University of Wisconsin--Madison, 1991.<br>Vita. Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 225-272).

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Sharp, Faith Christine. "Characterization of QSEA and QSED in the quorum sensing cascade of Enterohemorrhagic Escherichia coli." 2005. http://edissertations.library.swmed.edu/pdf/SharpF081105/SharpFaith.pdf.

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Chen, Li-Ming, and 陳立鉻. "Rapid Identification and Molecular Typing of Enterohemorrhagic Escherichia coli O157:H7." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/74064198128034331563.

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碩士<br>國立臺灣大學<br>農業化學研究所<br>88<br>The DNA sequence that includes entire stx2 gene of E. coli O157:H7 strain Y350-1 isolated from cattle faeces in Taiwan was determined. Both DNA and protein sequences have high identities of 99% with other E. coli O157:H7 stx2 gene in database. DNA sequence has 7 base pair differences whereas protein sequence of subunit A has one amino acid difference with the most similar sequence in database. A multiplex PCR with primers specific to hemolysn, intimin, Stx1, Stx2, O-antigen and flagella antigen gene hlyA, eaeA, stx1, stx2, rfb, and fliC was designed

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Chen, Li-Ming, and 陳立銘. "Rapid Identification and Molecular Typing of Enterohemorrhagic Escherichia coli O157:H7." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/89996932641527413163.

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碩士<br>國立臺灣大學<br>農業化學研究所<br>88<br>The DNA sequence that includes entire stx2 gene of E. coli O157:H7 strain Y350-1 isolated from cattle faeces in Taiwan was determined. Both DNA and protein sequences have high identities of 99% with other E. coli O157:H7 stx2 gene in database. DNA sequence has 7 base pair differences whereas protein sequence of subunit A has one amino acid difference with the most similar sequence in database. 　　A multiplex PCR with primers specific hemolysn, intimin, Stx1, Stx2，O-antigen and flagella antigen gene hlyA, eaeA, stx1, stx2, rfb, and fliC was designed and has a hig

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Huang, Ling-Hui, and 黃鈴惠. "GrlA Involved in the Pathogenesis of Enterohemorrhagic Escherichia coli O157:H7." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/67207402112893697672.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>93<br>The locus of enterocyte effacement (LEE) island is one of the virulence factors of enterohemorrhagic Escherichia coli (EHEC) O157:H7 which including proteins of type III secretion system (TTSS). EHEC infects and causes attaching and effacing lesion on host large intestinal cell thorugh the TTSS. Global regulator of LEE-activator (GrlA), which is located within the LEE, is a positive regulator of the LEE operons. However, the regulatory mechanism and the relationships among GrlA and the other two LEE-encoded regulators, GrlR and Ler, still await deciphering.

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Chang, Shu-Ting, and 張舒婷. "Dissecting the EspA Secretion Mechanism of Enterohemorrhagic Escherichia coli O157: H7." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/47673373893516858561.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>98<br>Enterohemorrhagic Escherichia coli (EHEC) O157:H7 causes hemorrhagic diarrhea. The major virulent factors reside in the locus of enterocyte effacement (LEE) in the bacterial genome. The LEE island encodes a set of genes for forming a specialized machinery called the type III secretion system (TTSS). TTSS secretes effectors, which collectively cause morphological changes of the infected cell, via a long filamentous structure that connects bacteria to the host cell membrane. This filament is composed of polymerized EspA and forms a hollow channel through which

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Yu, Sung-Liang, and 俞松良. "Interaction between Enterohemorrhagic Escherichia coli O157:H7 and Its Specific Phage AR1." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/62010991960089688456.

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博士<br>國立陽明大學<br>微生物暨免疫學研究所<br>87<br>AR1, a coliphage whose morphology and some essential genes are similar to those of T4 phage, infects Escherichia coli O157:H7 with high specificity. We characterized its two tail fiber proteins gp37 and gp38, and investigated host factors that confer this infection specificity. The host range determinants of T4-like phages and T2-like phages are gp37 and gp38, respectively. It is known that g37 of AR1 does not share similarity with that of any T-even phages except for the 5'' region coding for the first 56 amino acid residues. In the phage particles, gp38 co

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Chan, Chen-Hung, and 陳宏展. "Environmental factors on the growth and toxin production of enterohemorrhagic Escherichia coli." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/79777487723155764940.

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Wu, Ting-I., and 吳亭儀. "Characterization of l0050 in Pathogenicity Island of Enterohemorrhagic Escherichia coli O157:H7." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/39067890535771273950.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>94<br>Enterohemorrhagic Escherichia coli (EHEC) is a pathogen that attaches to host intestinal epithelium and efface brush border microvilli, forming A/E lesions. Many key virulence factors reside in the locus of bacterial chromosome named enterocyte effacement (LEE) island. The LEE island contains 41 genes and encodes a type III secretion system (TTSS). TTSS is commonly found in Gram negative pathogenic bacteria. The unique feature is a delivery of bacterial proteins capable of modulating host cellular functions. Recent studies have assigned functions for most ge

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蘇淑薇. "Characterization of gene Loo17 in pathogenicity island of enterohemorrhagic escherichia coli O157:H7." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/93929477561911154515.

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碩士<br>國立陽明大學<br>微生物暨免疫學研究所<br>91<br>Enterohemorrhagic Escherichia coli O157:H7 causes enterohemorrhagic diarrhea and a serious illness known as hemolytic-uremic syndrome. In the contact with epithelial cells, bacteria tightly associate with cells through a pedestal formation. Underneath the pedestal structure, cytoskeleton rearrangement has been observed. These pathogenic characteristics have been attributed to that this bacterium contains a pathogenic island located on the bacterial chromosome known as the locus for enterocyte effacement (LEE). There is a predicted cluster of 41 open reading

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Ting-ChenChou and 周廷蓁. "Functional Genomic Analysis of the Virulence of Enterohemorrhagic Escherichia coli in Caenorhabditis elegans." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/36q4v9.

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碩士<br>國立成功大學<br>生物化學暨分子生物學研究所<br>100<br>Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 is a major foodborne pathogen causing severe disease in humans worldwide. Three major groups of virulence factors of E. coli O157:H7 have been identified including the Shiga-like toxins, the products of the pathogenicity island called the locus of enterocyte effacement (LEE), and the products of the F-like plasmid pO157. Several strategies for therapy have been studied including the use of antibiotics and vaccination. However, there is no specific treatment for E. coli O157:H7 infection so far an

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Wu, Yi-Chih, and 吳宜之. "Analyzing Enterohemorrhagic Escherichia coli O157:H7 Pathogenicity Island at mRNA and Protein Levels." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/32144020522900189279.

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碩士<br>國立陽明大學<br>微生物暨免疫學研究所<br>90<br>Enterohemorrhagic Escherichia coli (EHEC) has emerged in recent years as the predominant cause of hemorrhagic colitis in humans. During infection, EHEC causes attaching and effacing (A/E) lesions on the intestinal cells. The genes involved in the formation of these A/E lesions are encoded within a chromosomal pathogenecity island named the locus of enterocyte effacement (LEE). The LEE comprises of 41 open reading frames (ORFs) organized in five major operons, LEE1, LEE2, LEE3, tir (LEE5), and LEE4. Among these ORFs, several have been identified, but others w

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Hsu, Pin-Yuan, and 徐彬源. "Characterization of L0045 in the Pathogenicity Island of Enterohemorrhagic Escherichia coli O157:H7." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/16140202955170438841.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>95<br>The locus of enterocyte effacement (LEE) island is the vital virulence factors of enterohemorrhagic Escherichia coli (EHEC) O157:H7 which includes proteins of type III secretion system (TTSS). EHEC infects and causes attaching and effacing lesion on host large intestinal epethilial cell thorugh the TTSS. LEE island has three main regulators, including global regulator of LEE-activator (GrlA), Global regulator of LEE-repressor (GrlR) and LEE-encoded regulator (Ler) , GrlA and Ler are the positive regulator of the LEE operons. On the other hand , GrlR is the n

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莊振樺. "Deducing tir properties that are different between escherichia coli of enterohemorrhagic and enteropathogenic virotypes." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/76322724588390171517.

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Yu, Yen-Chi, and 游硯棋. "The Role of l0045 in the Type III Secretion System of Enterohemorrhagic Escherichia coli." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/ctg8ce.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>97<br>Enterohemorrhagic E. coli (EHEC), which is an etiological agent for food-borne illnesses, is one of the pathogenic E. coli strains. Infection of EHEC often leads to bloody diarrhea and sometimes to serious diseases of hemolytic-uremic syndrome and acute kidney failure. EHEC infects large intestinal cells and forms typical histological lesions called attaching and effacing lesions (A/E lesion). The known mechanism is that the bacteria employ a type III secretion system to deliver several effector proteins into the infected cells. The effector proteins then tr

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Chiu, Hao-Chiech, and 邱浩傑. "Analysis of EspB Determinants Involved in Type III Secretion System of Enterohemorrhagic Escherichia coli." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/36697333417579270402.

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碩士<br>國立陽明大學<br>生物藥學研究所<br>88<br>Enterohemorrhagic E. coli (EHEC) can cause different presentations of disease in human. EHEC, like other Gram negative pathogenic bacteria, causes attachment and effacement lesion (A/E lesion) with infected epithelial cells. A type III protein secretion system is necessary for the formation of A/E lesion, and several proteins that are secreted by the system, including EspB (E. coli-secreted proteins B), are involved in inducing the formation of actin pedestals. EspB is secreted outside the bacteria and translocated to the membrane and cytosol of infected epithe

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Hsu, ching-Fang, and 許瀞方. "Detection of enterohemorrhagic Escherichia coli O157:H7 by using multiplex real-time PCR technology." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/17555476995842352456.

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碩士<br>國立臺灣大學<br>微生物與生化學研究所<br>92<br>Real-time polymerase chain reaction (PCR) assays have been developed for detection and quantification of pathogens in recent years. A multiplex real-time PCR assay was designed and evaluated on the ABI 7700 sequence detection system (TaqMan®) to detect enterohemorrhagic Escherichia coli O157:H7 in pure culture, feces and food samples. Two sets of primers and fluorescent probes were used for amplification and real-time detection of a 192-bp region of the rfbO157 gene encoding E. coli O157:H7-specific O-antigen, and 170-bp segment of stx2 gene encoding Shiga-

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Huang, Hui-Yu, and 黃惠鈺. "The role of EspFu in the pathogenic mechanism of Enterohemorrhagic Escherichia coli O157:H7." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/14691823302222872549.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>94<br>After enteropathogenic Escherichia coli (EHEC) infects cells, disease presentations such as attaching and effacing (AE) lesions occur. While microbial pathogens are attached to the epithelium of the host, the Tir molecules of bacteria are translocated to the host cell membrane and bind to the bacterial outer membrane adhesion molecule intimin. Translocation of Tir and other effector proteins is mediated by a type III secretion system of EHEC, and the resulting events include a disturbing of cellular signal pathways that regulate actin polymerization at the p

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Gao, Yi-Shun, and 高義順. "Investigation of Anomalous SDS-PAGE Migration of EscC in Enterohemorrhagic Escherichia coli O157:H7." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/73066695691720237571.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>99<br>Enterohemorrhagic E. coli (EHEC), a Gram-negative bacterium, which infects human gastrointestinal tract through undercooked beef or contaminated foods, is one of the pathogenic E. coli strains. Infection of EHEC could cause bloody diarrhea and sometimes lead to hemolytic-uremic syndrome and kidney failure. EHEC attacks the epithelial cells of intestinal tracts and results in typical histological lesions called attaching and effacing lesions (A/E lesion). The type three secretion system of EHEC plays an important to this lesion. Bacteria regulate a type three

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Hung, Tzu-Chun, and 洪子淳. "Characterization of L0053 Encoded in the Pathogenicity Island of Enterohemorrhagic Escherichia coli O157:H7." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/41396646220190792575.

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碩士<br>國立陽明大學<br>微生物及免疫學研究所<br>100<br>Enterohemorrhagic Escherichia coli (EHEC) utilizes Type III Secretion System (TTSS) to deliver virulent effectors, a result leading to cytoskeleton rearrangement and pedestal structure formation. This pedestal structure holds EHEC tightly to host cells and causes human epithelial cells becoming loosened and sparse, a pathogenesis named attaching and effacing lesion (A/E lesion). The effector proteins are encoded by genes clustered in the locus of enterocyte effacement (LEE) island in bacterial chromosome. The LEE island contains 41 open reading frames (ORFs

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Buryakina, Tatyana, and 棠雅. "Monitoring of HeLa Cells Revealed through Autofluorescence Lifetime upon Infection with enterohemorrhagic Escherichia coli." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/75206767510233325746.

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博士<br>國立陽明大學<br>生醫光電工程研究所<br>100<br>Bacterial infections and their influence on cellular physiology are one of the critical interests for researchers in different areas, such as microbiology, medicine, drug design, etc. due to the importance of thorough understanding of the processes related to the development of the efficient therapies. A number of different techniques are being applied for these studies, among which fluorescence spectroscopy has gained wide acceptance because of its high sensitivity and resolution. Being minimum invasive technique, autofluorescence spectroscopy allows studyi

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TSAY, Yu-Wei, and 蔡郁偉. "Comparative genome annotation for pathogenic entericbacteria phage analysis between enterohemorrhagic Escherichia coli and other bacteria." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/33675776228209603945.

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碩士<br>元智大學<br>資訊工程學系<br>92<br>In decades, the varieties of organisms were sequenced and annotated form laboratory accordingly. The bacterium Escherichia coli is one of the most comprehensive analyzed free-living organisms recently. The sequence、metabolic pathway and regulatory mechanism of these bacterium are well understanding to comparison with other bacterium. However, the mere sequenced of E. coli was not enough to understand the pathogenic mechanism precisely, nothing but it’s physical property or patient’s pathological diagnosis to make a compendium determination. We do not know the exac

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