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Journal articles on the topic 'Enterotoxinas'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Oliveira, Tereza Cristina Rocha Moreira de, Maria De Lourdes R. de Souza da Cunha, and Elisa Yoko Hirooka. "Enterotoxinas estafilocócicas." Semina: Ciências Agrárias 16, no. 1 (1995): 178. http://dx.doi.org/10.5433/1679-0359.1995v16n1p178.

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Viegas, Simone Assis Rosa de Aquino, Roberto Mitio Yanaguita, Edgar De Aquino Viegas, Luzinete Alves Silva, and Silvio Arruda Vasconcellos. "Identificação sorológica e caracterização das toxinas de estirpes de Escherichia coli isoladas de suínos com diarréia, criados no Estado de São Paulo." Brazilian Journal of Veterinary Research and Animal Science 29, supl. (1992): 394. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.1992.52009.

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Em cinco criações de suínos localizadas nos municípios de Bragança Paulista e Guarulhos, no Estado de São Paulo, Brasil, foram colhidas amostras de fezes de 85 leitões, com um a 30 dias de idade, apresentando quadro clínico de diarréia. A pesquisa de enterotoxina termoestável (STa) foi efetivada através do teste em camundongos recém-nascidos enquanto a demonstração da STb foi realizada em alça ligada de intestino de suíno. O sorogrupo 0149 foi constatado em 19 (22,35%) isolamentos dos quais 16 (18,82%) produziram enterotoxina STa e três (3,53%) produziram enterotoxinas STa e STb. O sorogrupo 0

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Silva, Juliana Fonseca Moreira, Amanda Campos Feitosa, and Rosimeire Mendes Rodrigues. "STAPHYLOCOCCUS AUREUS EM ALIMENTOS." DESAFIOS - Revista Interdisciplinar da Universidade Federal do Tocantins 4, no. 4 (2017): 15–31. http://dx.doi.org/10.20873/uft.2359-3652.2017v4n4p15.

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A intoxicação alimentar por estafilococos é uma das doenças transmitidas por alimentos (DTA) mais comuns e resulta da ingestão de enterotoxinas estafilocócicas (EE) pré-formadas em alimentos. Este trabalho teve como objetivo elaborar uma revisão sobre Staphylococcus em alimentos, surtos de intoxicação estafilocócica, enterotoxinas comumente envolvidas em surtos e métodos de identificação de enterotoxinas estafilocócicas. A metodologia empregada baseou-se na pesquisa qualitativa e sistemas de informação. No Brasil os dados do Sistema Nacional de Agravos e Notificações da Secretaria de Vigilânci

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Drummond, V. O., and S. Perecmanis. "Genes de enterotoxinas e perfil antimicrobiano de Escherichia coli isoladas de suínos hígidos no Distrito Federal." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 65, no. 4 (2013): 1005–9. http://dx.doi.org/10.1590/s0102-09352013000400010.

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Um total de 127 cepas de Escherichia coli foi isolado de suínos no Distrito Federal, testado para a presença de genes de enterotoxinas (STa, LT-I, LT-II, Stx1 e Stx2) e para resistência antimicrobiana. Das cepas isoladas, oito (6,3%) possuíam genes para enterotoxinas, sendo quatro (3,2%) positivas somente para LT-I, três (2,4%) somente para STa e uma (0,8%) positiva para STa e LT-I. Nenhuma das cepas isoladas apresentou genes para LT-II, Stx1 ou Stx2. Quanto ao perfil de resistência antimicrobiano, os antibióticos com maiores porcentagens de resistência foram lincomicina (100%), sulfonamidas (

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Costa, Juliana de Castro Beltrão da, Elaine Ibrahim de Freitas, Anderson Almeida de Lemos, et al. "Isolamento de Staphylococcus de queijo minas frescal e detecção de genes de enterotoxinas." Revista do Instituto Adolfo Lutz 71, no. 2 (2012): 250–58. http://dx.doi.org/10.53393/rial.2012.v71.32422.

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Neste trabalho foi realizado o isolamento de Staphylococcus spp. de queijo minas frescal, e para esta finalidade foi padronizado um protocolo multiplex PCR (M-PCR) para efetuar a detecção de genes de enterotoxinas clássicas (sea, seb, sec, sed e see) de Staphylococcus aureus, usando-se o gene femA como controle positivo para cepas de S. aureus. Foi testada a detecção direta de bactérias de amostras de queijo minas frescal e do alimento artificialmente contaminado. Cento e onze colônias (104 coagulase-positivas e sete coagulase-negativas) foram selecionadas e analisadas por M-PCR e, dentre essa

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Costa, F. N., and O. D. Rossi Junior. "ENTEROTOXIGENICIDADE DE ESPÉCIES DE AEROMONAS ISOLADAS EM DIFERENTES PONTOS DO FLUXOGRAMA DE ABATE DE FRANGOS." Arquivos do Instituto Biológico 74, no. 1 (2007): 5–9. http://dx.doi.org/10.1590/1808-1657v74p0052007.

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RESUMO Com o objetivo de verificar a capacidade enterotoxigênica “in vivo” de cepas de Aeromonas spp. isoladas em diferentes produtos e locais da planta de processamento de frangos, foram submetidas aos testes de inoculação intragástrica em camundongos lactentes e inoculação em alça ileal ligada de coelhos 75 cepas isoladas de penas, fezes, carcaças não evisceradas, evisceradas e resfriadas e água do pré-resfriamento. Das 75 cepas testadas, 31 (41,3%) produziram enterotoxinas na prova de inoculação em alça ileal ligada de coelhos, sendo 9 (29%) da espécie Aeromonashydrophila, 9 (29%) A. sobria

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Evelyn, Dayanara Atancuri Barreiro, Fernando Andrade Tacuri Carlos, and Gerardo Ortiz Tejedor Jonnathan. "Genes de Enterotoxinas de Staphylococcus aureus en superficies nosocomiales." REDIELUZ 11, Genes de Enterotoxinas de Staphylococcus aureus en superficies nosocomiales (2021): 65–72. https://doi.org/10.5281/zenodo.6800306.

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Sthapylococcus aureus, productor de enterotoxinas, es un patógeno causante de gran parte de intoxicaciones alimenticias a nivel mundial, al encontrarse en superficies nosocomiales puede afectar a pacientes vulnerables, lo que implica elevadas tasas de morbilidad y mortalidad, difíciles de controlar mucho más aún en países en desarrollo. Se identificó, la presencia de genes que codifican para enterotoxinas aislados de cepas de S. aureus en superficies nosocomiales de una institución de salud. La investigación fue de enfoque cualitativo, de

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Farfán Sari, Katherine Fernanda, Gabriela Rosario Romero Zhagui, and Jonnathan Gerardo Ortiz Tejedor. "Susceptibilidad antimicrobiana y enterotoxinas en Staphylococcus aureus aislado de queso fresco expendido en mercados municipales de la ciudad de Cuenca – Ecuador." Anatomía Digital 6, no. 4 (2023): 22–40. http://dx.doi.org/10.33262/anatomiadigital.v6i4.2704.

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Introducción. Las bacterias son microorganismos unicelulares causantes de múltiples enfermedades. En la actualidad constituyen un problema de salud pública debido a su alta tasa de resistencia a los antimicrobianos por diferentes mecanismos. Staphylococcus aureus es una de las especies predominantes en los seres humanos, asociada a factores de virulencia y dentro de estos, destacan enterotoxinas A, B, C, D, E, siendo causantes de Enfermedades Transmitidas por Alimentos (ETAs). Objetivo. Determinar la susceptibilidad antimicrobiana y enterotoxinas en Staphylococcus aureus aislado de queso fresc

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Seridan, B., M. R. Souza, J. R. Nicoli, et al. "Viabilidade de Staphylococcus aureus FRI S-6 e produção de SEB em queijo elaborado com adição de Lactobacillus rhamnosus e Lactococcus lactis." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 64, no. 2 (2012): 465–70. http://dx.doi.org/10.1590/s0102-09352012000200029.

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Staphylococcus aureus foi inoculado em queijos produzidos de forma estéril em laboratório, juntamente com Lactobacillus rhamnosus e Lactococcus lactis, isolados de queijo de coalho artesanal e identificados por PCR-ARDRA16S-23S. L. lactis foi capaz de reduzir a contagem de S. aureus no primeiro dia após produção (P<0,05) dos queijos de 3,3x10(7)UFC/g para 1,0x10(7)UFC/g. L. rhamnosus não impediu o crescimento de S. aureus. A presença das cepas acidoláticas, principalmente L. lactis, mostrou ainda potencial de inibição da produção de enterotoxina estafilocócica do tipo B, sendo que a concent

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Acosta, Atzel Candido, Sidney José dos Santos, Laís Albuquerque, Karla Danielle Almeida Soares, Rinaldo Aparecido Mota, and Elizabeth Sampaio de Medeiros. "Frequência de genes codificadores de toxinas em Staphylococcus aureus isolados de leite de tanques expansão comunitários." Pesquisa Veterinária Brasileira 37, no. 7 (2017): 691–96. http://dx.doi.org/10.1590/s0100-736x2017000700007.

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RESUMO: A capacidade de produção de toxinas pelo Staphylococcus aureus no leite e produtos derivados está relacionado com surtos de intoxicação alimentar. Objetivou-se nesta pesquisa, estudar a ocorrência de genes que codificam para enterotoxinas estafilocócicas (sea, seb, sed, seg, seh e sei) e toxinas α e β hemolítica (hla e hlb) em S. aureus isolados de 53 amostras de leite de tanques expansão comunitários no Estado de Alagoas, Brasil. Foram identificados 27 isolados (50,94%) como S. aureus pela amplificação do gene nuc. 13/27 isolados (48,1%) foram positivos para pelo menos um gene das ent

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Borges, Maria de Fatima, Renata Tieko Nassu, José Luiz Pereira, Ana Paula Colares de Andrade, and Arnaldo Yoshiteru Kuaye. "Perfil de contaminação por Staphylococcus e suas enterotoxinas e monitorização das condições de higiene em uma linha de produção de queijo de coalho." Ciência Rural 38, no. 5 (2008): 1431–38. http://dx.doi.org/10.1590/s0103-84782008000500037.

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Avaliou-se o perfil de contaminação por Staphylococcus e suas enterotoxinas e monitorou-se as condições de higiene em uma linha de produção de queijo de coalho por meio da técnica de bioluminescência (ATP). A população de Staphylococcus sp. variou de <1UFC mL-1, no leite pasteurizado a 1,5 x 10(7)UFC mL-1 no leite cru, enquanto que a de Staphylococcus coagulase-positiva oscilou entre <1UFC mL-1 no leite pasteurizado e 5,0 x 10(6)UFC mL-1 no leite cru. Staphylococcus coagulase-positiva foi detectada em 100% (25/25) das amostras de leite cru e em 8% (2/25) das amostras de queijos. Foram id

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Cardoso, H. F. T., L. S. Carmo, and N. Silva. "Detecção da toxina-1 da síndrome do choque tóxico em amostras de Staphylococcus aureus isoladas de mastite bovina." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 52, no. 1 (2000): 07–10. http://dx.doi.org/10.1590/s0102-09352000000100002.

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Este trabalho teve por objetivo caracterizar a produção da toxina da síndrome do choque tóxico (TSST-1) e de enterotoxinas estafilocócicas (SE) A, B, C e D em 127 amostras de S. aureus, isoladas de amostras de leite proveniente de vacas com mastite no Estado de Minas Gerais, entre 1994 e 1997. A verificação da produção de toxinas foi feita pela técnica de sensibilidade ótima em placa. Das 127 amostras testadas, 60 (47%) eram produtoras de TSST-1 e 54 (43%) produtoras de SE, 38 amostras produziram SED (30%), 24 SEB (19%), 8 SEC (6%) e 4 SEA (3%). Estes resultados trazem preocupações quanto à sa

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Fagundes, Helena, and Carlos Augusto Fernandes Oliveira. "Infecções intramamárias causadas por Staphylococcus aureus e suas implicações em paúde pública." Ciência Rural 34, no. 4 (2004): 1315–20. http://dx.doi.org/10.1590/s0103-84782004000400058.

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Neste trabalho, são apresentados os principais problemas decorrentes das infecções intramamárias (mastites) causadas por Staphylococcus aureus e as conseqüências para a saúde humana da veiculação de suas toxinas através do leite. o S. aureus destaca-se como um dos microorganismos mais importantes que podem ser transmitidos através dos alimentos. Assim, discute-se a possibilidade de veiculação de gastroenterite estafilocócica, não somente através do consumo de leite cru contaminado, mas também de leite tratado termicamente ou de derivados lácteos contendo enterotoxinas termoestáveis. São aprese

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Angarita-Merchán, Maritza, and Nuri Andrea Merchán Castellanos. "Genes codificantes para enterotoxinas de aislados estafilocócicos coagulasa negativos y coagulasa-positivos a partir muestras con mastitis bovina." Revista Investigación en Salud Universidad de Boyacá 5, no. 2 (2018): 205–18. http://dx.doi.org/10.24267/23897325.267.

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Introducción: La mastitis bovina es la inflamación de glándulas mamarias y tejidos secretores. El género Staphylococcus es el agente causal más importante, por su capacidad de producir diferentes factores de virulencia. Las enterotoxinas estafilocócicas son un grupo importante de toxinas que permiten al microorganismo invadir células y tejido huésped, siendo diseminadas a través de productos alimenticios y responsables de graves intoxicaciones alimentarias en el mundo. Objetivo: Determinar la presencia de genes codificantes para enterotoxinas estafilocócicas (SE); SEA, SEB, SEC, SED y SE

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Siqueira, Amanda Keller, Tatiana Salerno, Gustavo Henrique Batista Lara, et al. "Enterotoxin genes, multidrug resistance, and molecular typing of Staphylococcus spp. isolated from organic bovine milk." Brazilian Journal of Veterinary Research and Animal Science 54, no. 1 (2017): 81. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2017.109171.

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A emergência de estafilococos multirresistentes e resistentes à meticilina, isolados de animais, alimentos e humanos é uma preocupação em saúde pública. Esses micro-organismos produzem diferentes toxinas relacionadas à intoxicação alimentar em humanos. Este estudo caracterizou Staphylococcus spp. isolados em duas fazendas orgânicas no Brasil. Foram coletadas 259 amostras de leite em duas propriedades leiteiras orgânicas, nas quais 58 (22,4%) estirpes de Staphylococcus spp. foram isoladas. A maior sensibilidade dos isolados foi observada para ceftiofur e sulfametoxazol/trimetoprim em 96,6%. Em

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Rezende-Lago, N. C. M., O. D. Rossi Jr., A. M. C. Vidal-Martins, and L. A. Amaral. "Ocorrência de Bacillus cereus em leite integral e capacidade enterotoxigênica das cepas isoladas." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 59, no. 6 (2007): 1563–69. http://dx.doi.org/10.1590/s0102-09352007000600032.

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Pesquisaram-se a presença de Bacillus cereus e a produção de enterotoxinas produzidas por esses microrganismos em 120 amostras de diversos tipos de leite. Bacillus cereus foi isolado e identificado em 22 (73,3%), 15 (50,0%), 29 (96,7%) e quatro (13,3%) amostras de leite em pó, cru, pasteurizado e UAT (longa vida), respectivamente. Para a detecção de enterotoxinas pela técnica da alça ligada de coelho, foram positivos, respectivamente, três (13,6%), um (7,1%) e 10 (35,7%) microrganismos isolados das amostras de leite em pó, leite cru e leite pasteurizado. Pelo teste de aumento de permeabilidade

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Dias, N. L., D. C. B. Silva, D. C. B. S. Oliveira, A. A. Fonseca Junior, M. L. Sales, and N. Silva. "Detecção dos genes de Staphylococcus aureus, enterotoxinas e de resistência à meticilina em leite." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 63, no. 6 (2011): 1547–52. http://dx.doi.org/10.1590/s0102-09352011000600036.

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Realizou-se a detecção do gene de Staphylococcus aureus, de enterotoxinas e de resistência à meticilina com extração de DNA feita diretamente de amostras de leite. Das 200 amostras estudadas, 145 (72,5%) amplificaram o gene femA, e estas foram analisadas quanto à presença dos genes sea, seb, sec e mecA. Os genes das enterotoxinas mais prevalentes foram: sea (60%), seb (37,9%) e sec (6,9%). Foram encontradas 18 amostras de leite (11,0 %) com S. aureus portadores do gene mecA. A detecção de S. aureus diretamente do leite, sem a necessidade de isolamento bacteriano e a caracterização do potencial

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Lamaita, H. C., M. M. O. P. Cerqueira, L. S. Carmo, D. A. Santos, C. F. A. M. Penna, and M. R. Souza. "Contagem de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas e toxina da síndrome do choque tóxico em amostras de leite cru refrigerado." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 57, no. 5 (2005): 702–9. http://dx.doi.org/10.1590/s0102-09352005000500017.

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Analisaram-se 80 amostras de leite cru refrigerado a 4°C e estocado por 48 horas em tanques refrigeradores de propriedades rurais do estado de Minas Gerais quanto à contagem e identificação de Staphylococcus sp. e detecção de enterotoxinas estafilocócicas (SE) e da toxina da síndrome do choque tóxico (TSST-1). Staphylococcus sp. foi detectado em 100% das amostras de leite de tanque refrigerador em contagens que variaram de 1,0 × 10(5) a 2,5 × 10(7) UFC/ml (média = 5,60 log UFC/ml; s = 0,53 e CV = 9,5%). Isolaram-se e identificaram-se 436 estirpes como: S. aureus, S hyicus, S. epidermidis, S. i

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LOPES, HELENA RODRIGUES, ALBA LUCIA SOLINO NOLETO, MARC DIAZ de LAS HERAS, and MERLIN S. BERGDOLL. "Selective Enterotoxin Production in Foods by Staphylococcus aureus Strains That Produce More Than One Enterotoxin." Journal of Food Protection 56, no. 6 (1993): 538–40. http://dx.doi.org/10.4315/0362-028x-56.6.538.

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Staphylococcus aureus strain S-6, producer of enterotoxins A and B; strain FRI-913, producer of enterotoxins A, C, and E; and strain FRI-196E, producer of enterotoxins A and D, were grown in milk (pH 6.8), potato salad with mayonnaise, and boiled egg white (pH 8.0–8.5). Enterotoxins were detectable in milk incubated at 26°C when colony counts were less than 106. Enterotoxin production by strain S-6 was detectable in potato salad at 106 CFU at both 26 and 37°C. Enterotoxin A production by strain FRI-913 was detectable at approximately 106 CFU/g, whereas enterotoxin C was detectable only when gr

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Aguas, Elizabeth M., Ivanna N. Jiménez, Elkin M. Iguaran, and Keyla P. Morales. "Importancia en Salud Pública y modelamiento de Staphylococcus Aureus en alimentos." Mente Joven 6 (December 1, 2017): 36–53. http://dx.doi.org/10.18041/2323-0312/mente_joven.0.2017.3668.

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Staphylococcus aureus es uno de los principales agentes causantes de ETA en Colombia y en el mundo. Este microorganismo causa intoxicación, la cual se debe a la ingestión de alimentos contaminados con enterotoxinas termoestables producidas por esta bacteria. Actualmente, existen herramientas predictivas basadas en conceptos matemáticos que permiten estimar del riesgo microbiológico y determinar la vida útil de una matriz alimenticia. Así, se realizó una revisión bibliográfica de la importancia de S. aureus como agente etiológico y de las acciones realizadas a través del tiempo para predecir su

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Sabioni, José Geraldo, Elisa Yoko Hirooka, and Maria de Lourdes R. de Souza. "Intoxicação alimentar por queijo Minas contaminado com Staphylococcus aureus." Revista de Saúde Pública 22, no. 5 (1988): 458–61. http://dx.doi.org/10.1590/s0034-89101988000500010.

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Relata-se surto de intoxicação alimentar ocorrido em julho de 1987, na cidade de Ouro Preto, MG (Brasil). O alimento causador foi um queijo Minas, contaminado por Staphylococcus aureus ao nível de 9,3 x 10(7) UFG/g. Detectaram-se cepas produtoras de enterotoxinas do tipo A,B,D e E. A amostra analisada revelou contaminação por coliformes fecais acima de 1,1 x 10(5)/g(NMP), mas não continha Salmonella.Devido aos sintomas característicos e à elevada contaminação, concluiu-se que o Staphylococcus aureus foi o patogênico responsável pelo surto.

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Bergdoll, Merlin S. "Staphylococcus aureus." Journal of AOAC INTERNATIONAL 74, no. 4 (1991): 706–10. http://dx.doi.org/10.1093/jaoac/74.4.706.

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Abstract The analytical methods for the detection of the staphylococcal enterotoxins can be divided into 2 categories: (1) methods for detection of enterotoxin-producing staphylococcal strains; (2) methods for detection of enterotoxin in foods. Gel diffusion methods (Ouchterlony, microslide), in which the enterotoxin produced by any given strain is compared to one of the identified enterotoxins, are used most frequently for strain testing. The sensitivity of these methods is from 0.1 to 0.5 μg enterotoxin/mL, which is normally adequate to determine the enterotoxigenicity of strains. The method

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PEREIRA, Maria Lúcia, Luiz Simeão CARMO, and José Luiz PEREIRA. "COMPORTAMENTO DE ESTAFILOCOCOS COAGULASE NEGATIVOS PAUCIPRODUTORES DE ENTEROTOXINAS, EM ALIMENTOS EXPERIMENTALMENTE INOCULADOS." Ciência e Tecnologia de Alimentos 21, no. 2 (2001): 171–75. http://dx.doi.org/10.1590/s0101-20612001000200009.

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Ernandez, Djair, and Ernesto Hofer. "Caracterização de sorotipos de Escherichia coli potencialmente produtores de enterotoxinas em alimentos." Memórias do Instituto Oswaldo Cruz 82, no. 1 (1987): 7–13. http://dx.doi.org/10.1590/s0074-02761987000100002.

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Em 137 amostras de alimentos de diferentes origens (animal e vegetal) foi investigada a ocorrência de sorotipos de Escherichia coli mais comumente descritos como produtores de enterotoxinas. A análise sorológica dos antígenos somáticos, de envoltórios e flagelares nas 265 culturas isoladas, resultou na identificação de 34 amostras distribuídas em doze sorotipos e oriundas de 24 produtos de origem animal. Outros aspectos foram analisados, visando associá-los como possíveis marcadores epidemiológicos. Assim, na biotipificação, verificou-se o perfil das 34 amostras diante da melibiose, rafinose,

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Gemeda, Shibabaw Tadesse, Adey Feleke Desta, Sirak Robele Gari, Jana Jass, Wude Mihret Woldemedhin, and Misrak Netsere Haileyesus. "Enterotoxins as a molecular marker of water quality." Water Practice and Technology 17, no. 2 (2022): 623–36. http://dx.doi.org/10.2166/wpt.2022.016.

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Abstract Safety of improved water supplies using enterotoxins as a molecular marker is evaluated. Water samples were collected from 248 households and tested for enterotoxins using polymerase chain reaction (PCR). The relationships between the presence of at least one enterotoxin and independent variables were investigated using Chi-square (χ2), Fisher's exact test and binary logistic regression. Some 156 enterotoxin biomarkers were detected, 39% of samples had at least one, and 17% had multiple varieties. EAST1 was detected in the highest proportion of samples (33%) and Sta in the lowest (2%)

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Wiśniewski, Patryk, Joanna Gajewska, Anna Zadernowska, and Wioleta Chajęcka-Wierzchowska. "Identification of the Enterotoxigenic Potential of Staphylococcus spp. from Raw Milk and Raw Milk Cheeses." Toxins 16, no. 1 (2023): 17. http://dx.doi.org/10.3390/toxins16010017.

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This study aimed to genotypic and phenotypic analyses of the enterotoxigenic potential of Staphylococcus spp. isolated from raw milk and raw milk cheeses. The presence of genes encoding staphylococcal enterotoxins (SEs), including the classical enterotoxins (sea-see), non-classical enterotoxins (seg-seu), exfoliative toxins (eta-etd) and toxic shock syndrome toxin-1 (tst-1) were investigated. Isolates positive for classical enterotoxin genes were then tested by SET-RPLA methods for toxin expression. Out of 75 Staphylococcus spp. (19 Staphylococcus aureus and 56 CoNS) isolates from raw milk (49

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Sha, Jian, E. V. Kozlova, and A. K. Chopra. "Role of Various Enterotoxins in Aeromonas hydrophila-Induced Gastroenteritis: Generation of Enterotoxin Gene-Deficient Mutants and Evaluation of Their Enterotoxic Activity." Infection and Immunity 70, no. 4 (2002): 1924–35. http://dx.doi.org/10.1128/iai.70.4.1924-1935.2002.

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ABSTRACT Three enterotoxins from the Aeromonas hydrophila diarrheal isolate SSU have been molecularly characterized in our laboratory. One of these enterotoxins is cytotoxic in nature, whereas the other two are cytotonic enterotoxins, one of them heat labile and the other heat stable. Earlier, by developing an isogenic mutant, we demonstrated the role of a cytotoxic enterotoxin in causing systemic infection in mice. In the present study, we evaluated the role of these three enterotoxins in evoking diarrhea in a murine model by developing various combinations of enterotoxin gene-deficient mutan

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Sékaly, R. P., G. Croteau, M. Bowman, P. Scholl, S. Burakoff, and R. S. Geha. "The CD4 molecule is not always required for the T cell response to bacterial enterotoxins." Journal of Experimental Medicine 173, no. 2 (1991): 367–71. http://dx.doi.org/10.1084/jem.173.2.367.

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T cells respond in a V beta-restricted fashion to bacterial enterotoxins bound to major histocompatibility complex (MHC) class II molecules. The requirement for CD4 in MHC class II-restricted T cell responses is very well established. We have assessed the role of CD4 in the T cell response to the bacterial enterotoxins Staphylococcal enterotoxin A (SEA), SEB, and toxic shock syndrome toxin 1. Three CD4- murine T cell hybridomas were transfected with the human CD4 molecule and assayed for interleukin 2 production in the presence of accessory cells bearing human MHC class II molecules and of the

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Valihrach, L., K. Demnerová, R. Karpíšková, and I. Melenová. "The expression of selected genes encoding enterotoxins in Staphylococcus aureus strains." Czech Journal of Food Sciences 27, Special Issue 2 (2010): 56–65. http://dx.doi.org/10.17221/208/2009-cjfs.

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Staphylococcus aureus is an important food-borne pathogen, which produces many toxic substances that cause a variety of illnesses. Some strains of S. aureus produce thermostable enterotoxins that can be responsible for alimentary intoxication. The aim of this work was to establish a protocol for the study of 9 enterotoxin genes expression (sea-sej). First, a method for the detection of genes encoding enterotoxins was established and then a method for the determination of the expression of these genes was optimised, using a range of the PCR techniques (multiplex, touchdown and real-time). The e

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SU, YI-CHENG, and AMY C. LEE WONG. "Current Perspectives on Detection of Staphylococcal Enterotoxins." Journal of Food Protection 60, no. 2 (1997): 195–202. http://dx.doi.org/10.4315/0362-028x-60.2.195.

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Staphylococcal food poisoning is one of the most economically important food-borne diseases in the United States, costing approximately $1.5 billion each year in medical expenses and loss of productivity. The amount of staphylococcal enterotoxin required to cause illness in humans depends on the susceptibility of the individuals. As little as 0.5 to 0.75 ng/ml of enterotoxin A in chocolate milk was shown to be able to cause illness in school children. Many methods have been developed for the detection of enterotoxins: immunological and biological assays. Immunological assays are more sensitive

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KIM, JUNG-BEOM, JAI-MOUNG KIM, SO-YEONG KIM, et al. "Comparison of Enterotoxin Production and Phenotypic Characteristics between Emetic and Enterotoxic Bacillus cereus." Journal of Food Protection 73, no. 7 (2010): 1219–24. http://dx.doi.org/10.4315/0362-028x-73.7.1219.

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Bacillus cereus was divided into emetic toxin (cereulide)– and enterotoxin-producing strains, but emetic toxin–producing B. cereus is difficult to detect immunochemically. Screening methods for emetic toxin–producing B. cereus are needed. The objectives of this study were to identify and detect emetic toxin–producing B. cereus among 160 B. cereus strains, and to compare enterotoxin production and phenotypic characteristics between the emetic toxin–producing and enterotoxin-producing strains. Forty emetic toxin–producing B. cereus strains were determined with high-pressure liquid chromatography

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Necidová, Lenka, Šárka Bursová, Danka Haruštiaková, and Kateřina Bogdanovičová. "Evaluation of Staphylococcus aureus growth and staphylococcal enterotoxin production in delicatessen and fine bakery products." Acta Veterinaria Brno 91, no. 4 (2022): 417–25. http://dx.doi.org/10.2754/avb202291040417.

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Staphylococcal food poisoning is one of the most prevalent causes of foodborne intoxication worldwide. Sandwiches and desserts are susceptible to contamination by S. aureus due to the high proportion of manual work during their production. Our study aimed to evaluate the impact of storage conditions on staphylococcal enterotoxin production in sandwiches and buttercream puffs. Foods were inoculated with different S. aureus strains capable of producing type A, B, and C staphylococcal enterotoxins and incubated at 15, 25, and 30 °C. During the storage, samples were analysed for S. aureus counts a

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Al Bustan, M. A., E. E. Udo, and T. D. Chugh. "Nasal carriage of enterotoxin-producingStaphylococcus aureusamong restaurant workers in Kuwait City." Epidemiology and Infection 116, no. 3 (1996): 319–22. http://dx.doi.org/10.1017/s0950268800052638.

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SUMMARYEnterotoxin-producingStaphylococcus aureusis a common cause of staphylococcal food poisoning. To determine the incidence of carriage of enterotoxin-producingS. aureusin a sample of the healthy population in Kuwait city, restaurant workers in the city were screened for nasal carriage ofS. aureus. 26·6% of 500 workers studied carriedS. aureusand 86·6% of theS. aureusproduced staphylococcal enterotoxins. 28 % produced enterotoxin A, 28·5 % produced enterotoxin B, 16·4% produced enterotoxin C and 3·5% produced enterotoxin D. Ten isolates produced both enterotoxins A and B or A and C. 73 % o

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Tallent, Sandra M., Jeffrey A. DeGrasse, Ningyan Wang, Daiva M. Mattis, and David M. Kranz. "Novel Platform for the Detection of Staphylococcus aureus Enterotoxin B in Foods." Applied and Environmental Microbiology 79, no. 5 (2012): 1422–27. http://dx.doi.org/10.1128/aem.02743-12.

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ABSTRACTStaphylococcal contamination of food products and staphylococcal food-borne illnesses continue to be a problem worldwide. Screening of food for the presence ofStaphylococcus aureusand/or enterotoxins using traditional methods is laborious. Reliable and rapid multiplex detection methods from a single food extract or culture supernatant would simplify testing. A fluorescence-based cytometric bead array was developed for the detection of staphylococcal enterotoxin B (SEB), using magnetic microspheres coupled with either an engineered, enterotoxin-specific Vβ domain of the T-cell receptor

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Cestari, Silvia Emanoele, Paulo Alfonso Schuroff, Nicole Ribeiro De Lima, Tatiane Das Neves Burgos, Angélica Marim Lopes Dambrozio, and Jacinta Sanchez Pelayo. "Caracterização genotípica de fatores de virulência de Escherichia Coli enterotoxigênica isoladas de água para consumo humano." Revista de Ciências Médicas e Biológicas 15, no. 2 (2016): 139. http://dx.doi.org/10.9771/cmbio.v15i2.13775.

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<p>Introdução: As doenças veiculadas pela água são principalmente causadas por patógenos entéricos, sendo o patotipo Escherichia coli enterotoxigênica (ETEC) de grande importância em países em desenvolvimento. Objetivo: Detectar a presença de genes de virulência relacionados à ETEC em cepas de E. coli isoladas de água para consumo humano. Metodologia: 295 cepas de E. coli obtidas de água de 27 municípios do norte do Estado do Paraná, Brasil, entre fevereiro e dezembro de 2005, foram testadas quanto a presença de genes que codificam para as enterotoxinas LT-I (lt-I), ST-a (st-a) e ST-b (s

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Cestari, Silvia Emanoele, Paulo Alfonso Schuroff, Nicole Ribeiro De Lima, Tatiane Das Neves Burgos, Angélica Marim Lopes Dambrozio, and Jacinta Sanchez Pelayo. "Caracterização genotípica de fatores de virulência de Escherichia Coli enterotoxigênica isoladas de água para consumo humano." Revista de Ciências Médicas e Biológicas 15, no. 2 (2016): 139. http://dx.doi.org/10.9771/2236-5222cmbio.v15i2.13775.

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<p>Introdução: As doenças veiculadas pela água são principalmente causadas por patógenos entéricos, sendo o patotipo Escherichia coli enterotoxigênica (ETEC) de grande importância em países em desenvolvimento. Objetivo: Detectar a presença de genes de virulência relacionados à ETEC em cepas de E. coli isoladas de água para consumo humano. Metodologia: 295 cepas de E. coli obtidas de água de 27 municípios do norte do Estado do Paraná, Brasil, entre fevereiro e dezembro de 2005, foram testadas quanto a presença de genes que codificam para as enterotoxinas LT-I (lt-I), ST-a (st-a) e ST-b (s

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Lapeyre, Christiane, Marie-Noelle De Solan, and Xavier Drouet. "Immunoenzymatic Detection of Staphylococcal Enterotoxins: International Interlaboratory Study." Journal of AOAC INTERNATIONAL 79, no. 5 (1996): 1095–101. http://dx.doi.org/10.1093/jaoac/79.5.1095.

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Abstract An international interlaboratory study was performed by 13 laboratories to validate a commercially available, rapid enzyme immunoassay for detection of staphylococcal enterotoxins A, B, C, D, and E in foods. The 5 enterotoxin serotypes were detected at a level of 0.5 ng/g in mushrooms and ravioli, 0.8 ng/g in meat, 1 ng/mL in milk, and 1.5 ng/g in raw milk cheese when these foods were artificially contaminated with enterotoxin A. Enterotoxins A, B, C, D, or E were also detected in culture supernatants with no protein A interference when normal rabbit serum was used. This method was va

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Mohammed, Srwa A., Mohammed I. J. Al-ani, Dereh L. Mohammed, Lina R. Salar, and Banaz M. Rasul. "Inhibition of Staphylococcus aureus enterotoxin genes by using plant extracts." Innovaciencia Facultad de Ciencias Exactas Físicas y Naturales 6, no. 2 (2018): 1–8. http://dx.doi.org/10.15649/2346075x.469.

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Introduction: Enterotoxigenic Staphylococcus aureus is an important pathogen that causes septicemia and bacteremia and is often associated with serious complications, such as endocarditis and osteomyelitis. Some Staphylococcus enterotoxins require only minute quantities to be toxic in humans. The present study focused on investigation how to remove this problematic issue. Objectives: This study was conducted to inhibit S. aureus enterotoxin genes that obtained from positive blood culture bottles of patients at the pediatric hospital in Sulaimania city. Methods: Twenty five isolates of S. aureu

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Rossi Júnior, O. D., L. A. Amaral, A. Nader Filho, and R. P. Schocken-Iturrino. "Enterotoxigenicidade de cepas de Aeromonas sp. isoladas em diferentes pontos do fluxograma de abate bovino." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 53, no. 5 (2001): 589–94. http://dx.doi.org/10.1590/s0102-09352001000500014.

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Com o objetivo de verificar a capacidade enterotoxigênica de cepas de Aeromonas sp. isoladas em diferentes produtos e locais no fluxograma de abate bovino, foram testadas 102 cepas (18 da espécie A. hydrophila, 65 da espécie A. caviae e 19 atípicas) ante os testes de inoculação intragástrica em camundongo lactente e em alça intestinal ligada de coelho. Revelaram-se como produtoras de enterotoxinas três (16,7%) cepas da espécie A. hydrophila, originárias das mãos do manipulador antes que ele iniciasse seus trabalhos e da carne desossada pronta para o consumo, e uma (1,5%) da espécie A. caviae,

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Chajęcka-Wierzchowska, Wioleta, Joanna Gajewska, Patryk Wiśniewski, and Anna Zadernowska. "Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food." Pathogens 9, no. 9 (2020): 734. http://dx.doi.org/10.3390/pathogens9090734.

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Although coagulase-positive staphylococci are considered to be the main factor responsible for food poisoning, an increasing role for the coagulase-negative staphylococci in the production of enterotoxins has been observed in recent years. This study was conducted to assess the occurrence of genes responsible for the production of staphylococcal enterotoxins (SE), enterotoxin-like toxins (SEI) and toxic shock syndrome toxin-1 (TSST-1) in coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food from bars and restaurants. One hundred and eighteen CoNS strains were tested using pol

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Lampugnani, Camila, Maike Taís Maziero Montanhini, Maria Emilene Martino Campos‐Galvão, Luis Augusto Nero, and Luciano dos Santos Bersot. "Enterotoxins production, biofilm formation and antimicrobial resistance of Staphylococcus aureus strains isolated from refrigerated raw cow milk." Acta Scientiarum. Technology 42 (November 29, 2019): e45231. http://dx.doi.org/10.4025/actascitechnol.v42i1.45231.

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This study aimed to isolate Staphylococcus aureus in refrigerated raw cow milk, and identify the presence of enterotoxin-expression genes, enterotoxin production and adherence ability, and antimicrobial resistance potential of the isolated strains. Fifty raw milk samples obtained in different dairy farms were analyzed for S. aureus and evaluated in the isolates the presence of genes associated with the production of major staphylococcal enterotoxins and biofilm formation. In vitro assays were also performed to evaluate the production of enterotoxins and adherence ability, and the antimicrobial

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Melconian, A. K., J. Fleurette, and Y. Brun. "Studies on staphylococci from toxic shock syndrome in France, 1981–1983." Journal of Hygiene 94, no. 1 (1985): 23–29. http://dx.doi.org/10.1017/s002217240006109x.

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SUMMARYStaphylococci from 22 cases of toxic shock syndrome with onsets between 1981 and March 1983 have been studied. Another four cases were detected by abstract surveillance. Three of these patients died. The case histories show that the syndrome occurs in women during menstruation as well as in males and in children, and is associated withStaphylococcus aureusinfections.The production of enterotoxins (A, B, C) and toxic shock toxin byS. aureusisolates from toxic shock syndrome was investigated. Twenty-two of the 23 isolates were found to be toxigenie: 7 produced enterotoxin A, 8 produced en

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Kadhim, B. A., M. A. Essa, and M. R. Sabbah. "PREVALENCE OF ENTEROTOXIN TYPE A IN STAPHYLOCOCCUS AUREUS ISOLATED FROM DIFFERENT SOURCES." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 53, no. 6 (2022): 1325–30. http://dx.doi.org/10.36103/ijas.v53i6.1648.

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This research was aimed to investigate the prevalence of enterotoxin type A in (82) Staphylococcus aureus isolates from different sources(Pathogenic, Soil, Food, Tape water& Nasal swabs from Normal source), the results had shown the capacity of (58) isolates (70.73%) to produce the enterotoxin by using culture method (in solid and liquid medium), concerning the liquid medium, the results conducted that there was a significant differences at P£ 0.05, among bacterial isolates in the amount of produced enterotoxin in terms of optical density. By using serological ELISA specific kit, the resul

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CENCI-GOGA, B. T., M. KARAMA, P. V. ROSSITTO, R. A. MORGANTE, and J. S. CULLOR. "Enterotoxin Production by Staphylococcus aureus Isolated from Mastitic Cows." Journal of Food Protection 66, no. 9 (2003): 1693–96. http://dx.doi.org/10.4315/0362-028x-66.9.1693.

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Staphylococcus aureus is an important cause of mastitis in cows. The ability of S. aureus strains to produce one or more enterotoxins in milk and dairy products is linked to staphylococcal food poisoning. To determine whether staphylococci causing bovine mastitis could cause human foodborne intoxication, the production of staphylococcal enterotoxins A through D (SEA, SEB, SEC, and SED) by 160 S. aureus isolates was evaluated with the use of a reverse passive latex agglutination enterotoxin kit. All S. aureus strains were isolated over a 9-month period from 2,343 routine submissions of a compos

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Thomas, Damien, Olivier Dauwalder, Virginie Brun та ін. "Staphylococcus aureus Superantigens Elicit Redundant and Extensive Human Vβ Patterns". Infection and Immunity 77, № 5 (2009): 2043–50. http://dx.doi.org/10.1128/iai.01388-08.

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ABSTRACT Staphylococcus aureus can produce a wide variety of exotoxins, including toxic shock syndrome toxin 1 (TSST-1), staphylococcal enterotoxins, and staphylococcal enterotoxin-like toxins. These toxins share superantigenic activity. To investigate the β chain (Vβ) specificities of each of these toxins, TSST-1 and all known S. aureus enterotoxins and enterotoxin-like toxins were produced as recombinant proteins and tested for their ability to induce the selective in vitro expansion of human T cells bearing particular Vβ T-cell receptors (TCR). Although redundancies were observed between th

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SCHUBERT, JUSTYNA, SYLWIA KRAKOWIAK, and JACEK BANIA. "Production of staphylococcal enterotoxins in food." Medycyna Weterynaryjna 74, no. 1 (2018): 16–22. http://dx.doi.org/10.21521/mw.5837.

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Staphylococcal food poisoning results from ingestion of food contaminated with toxins produced by enterotoxigenic Staphylococcus aureus strains. Common symptoms of this intoxication include vomiting, diarrhea, and abdominal cramps. Staphylococcal enterotoxins are resistant to heat and a number of environmental factors. Certain cheeses, milk powder, and whey powder are the only foodstuffs that are being routinely examined for the presence of staphylococcal enterotoxins SEA-SEE. The newly identified enterotoxins are not included in the current examination scheme. Enterotoxin-producing staphyloco

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Hassan, Riyam Wissam, and Azhar Noory Hussein. "Molecular Study of Enterotoxin Genes of Staphylococcus aureus Isolated Locally from Al-Diwaniyah City." Medical Journal of Babylon 22, no. 1 (2025): 77–80. https://doi.org/10.4103/mjbl.mjbl_207_23.

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Abstract Background: Following Salmonella, Staphylococcus aureus has been identified as the second most frequent origin of foodborne disease outbreaks. It was discovered that there are five main forms of enterotoxins, named the classic staphylococcal enterotoxin A, staphylococcal enterotoxin B, staphylococcal enterotoxin C, staphylococcal enterotoxin D, and staphylococcal enterotoxin E. Objectives: This study aims to isolate S. aureus and, then, study the molecular characterization of the enterotoxin genes that these bacteria possess by using the polymerase chain reaction (PCR) technique to de

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Jung, Hye-Ri, and Young Ju Lee. "Characterization of Virulence Factors in Enterotoxin-Producing Staphylococcus aureus from Bulk Tank Milk." Animals 12, no. 3 (2022): 301. http://dx.doi.org/10.3390/ani12030301.

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Staphylococcus aureus, a persistent mastitis-causing pathogen, produces various virulence factors, including enterotoxins. This study analyzed the genetic characteristics of bovine-mastitis-related virulence factors to evaluate the potential pathogenesis of S. aureus isolated from bulk tank milk. Among 93 S. aureus isolates from 396 dairy farms operated by 3 dairy companies in Korea, 40 (43.0%) isolates carried one or more enterotoxin genes. Moreover, S. aureus carrying enterotoxin genes showed a higher prevalence in all virulence genes tested in this study except for pvl and lukM, which were

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Piechota, M., B. Kot, E. Zdunek, J. Mitrus, J. Wicha, and M. K. Wolska. "Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment." Polish Journal of Veterinary Sciences 17, no. 3 (2014): 407–11. http://dx.doi.org/10.2478/pjvs-2014-0058.

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AbstractThe aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 gene

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Cheng, K.-J., Y.-Y. Xu, M.-L. Zhou, S.-H. Zhou, and S.-Q. Wang. "Role of local allergic inflammation and Staphylococcus aureus enterotoxins in Chinese patients with chronic rhinosinusitis with nasal polyps." Journal of Laryngology & Otology 131, no. 8 (2017): 707–13. http://dx.doi.org/10.1017/s0022215117001335.

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AbstractObjective:To investigate the role of local allergic inflammation and Staphylococcus aureus enterotoxins in chronic rhinosinusitis with nasal polyps.Methods:This study included 36 patients with chronic rhinosinusitis with nasal polyps and 18 controls. Total immunoglobulin E, eosinophil cationic protein, staphylococcal enterotoxin types A and B specific immunoglobulin E, staphylococcal enterotoxin types A and B, and myeloperoxidase levels were determined.Results:Four patients with chronic rhinosinusitis with nasal polyps had a local allergy. All chronic rhinosinusitis with nasal polyps p

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