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Relevant bibliographies by topics / Envelope protein

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Academic literature on the topic 'Envelope protein'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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Journal articles on the topic "Envelope protein"

1

Pan, Yong, Jiming Yan, Yinong Zhang, Jiasheng Lin, Zhiquan Liang, and Jingchen Sun. "Centrifugation-Based Purification Protocol Optimization Enhances Structural Preservation of Nucleopolyhedrovirus Budded Virion Envelopes." Insects 16, no. 4 (2025): 424. https://doi.org/10.3390/insects16040424.

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The structural integrity of viral envelopes is a critical determinant of infectivity for enveloped viruses, directly influencing vector stability, functional accuracy of surface-displayed epitopes, and preservation of native conformational states required for membrane protein studies. However, conventional purification methods often disrupt envelope integrity and cause envelope proteins to lose their activity. Here, we systematically compared discontinuous, continuous, and optimized continuous sucrose density gradient centrifugation protocols for purifying Autographa californica multiple nucle

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Kalmokoff, M. L., J. W. Austin, M. F. Whitford, and R. M. Teather. "Characterization of a major envelope protein from the rumen anaerobeSelenomonas ruminantiumOB268." Canadian Journal of Microbiology 46, no. 4 (2000): 295–303. http://dx.doi.org/10.1139/w99-149.

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Cell envelopes from the Gram-negative staining but phylogenetically Gram-positive rumen anaerobe Selenomonas ruminantium OB268 contained a major 42 kDa heat modifiable protein. A similarly sized protein was present in the envelopes of Selenomonas ruminantium D1 and Selenomonas infelix. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of Triton X-100 extracted cell envelopes from S. ruminantium OB268 showed that they consisted primarily of the 42 kDa protein. Polyclonal antisera produced against these envelopes cross-reacted only with the 42 kDa major envelope proteins in both S. rumin

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Huang, Po-Yu, Jiann-Horng Leu, and Li-Li Chen. "A newly identified protein complex that mediates white spot syndrome virus infection via chitin-binding protein." Journal of General Virology 95, no. 8 (2014): 1799–808. http://dx.doi.org/10.1099/vir.0.064782-0.

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White spot syndrome virus (WSSV) is a large enveloped virus which has caused severe mortality and huge economic losses in the shrimp farming industry. The enveloped virus must be combined with the receptors of the host cell membrane by the virus envelope proteins. In the case of WSSV, binding of envelope proteins with receptors of the host cell membrane was discovered in a number of previous studies, such as VP53A and 10 other proteins with chitin-binding protein (CBP), VP28 with Penaeus monodon Rab7, VP187 with β-integrin, and so on. WSSV envelope proteins were also considered capable of form

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Lin, Lo-Wei, Michael S. Denison, and Robert H. Rice. "Woodsmoke Extracts Cross-Link Proteins and Induce Cornified Envelope Formation without Stimulating Keratinocyte Terminal Differentiation." Toxicological Sciences 183, no. 1 (2021): 128–38. http://dx.doi.org/10.1093/toxsci/kfab071.

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Abstract Air pollution poses a serious risk to human health. To help understand the contribution of smoke from wood burning to the harmfulness of air pollution toward the skin, we studied the effects of liquid smoke, aqueous extracts of wood smoke condensate, a commercially available food flavor additive, in cultured keratinocytes. We report that liquid smoke can react with and cross-link keratinocyte cellular proteins, leading to abnormal cross-linked envelope formation. Instead of inducing genes ordinarily involved in terminal differentiation, liquid smoke induced expression of genes associa

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van Hulten, Mariëlle C. W., Martin Reijns, Angela M. G. Vermeesch, Fokko Zandbergen, and Just M. Vlak. "Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins." Journal of General Virology 83, no. 1 (2002): 257–65. http://dx.doi.org/10.1099/0022-1317-83-1-257.

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White spot syndrome virus (WSSV) infects penaeid shrimp and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double-stranded DNA genome of 293 kbp. The virion envelope contains two major proteins of 28 (VP28) and 19 kDa (VP19) and the nucleocapsid consists of three major proteins of 26 (VP26), 24 (VP24) and 15 kDa (VP15). Study on the morphogenesis of the WSSV particle requires the genomic identification and chemical characterization of these WSSV virion proteins. An internal amino acid sequence of envelope protein VP19 was obtained

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Hyllner, S. J., and C. Haux. "Immunochemical detection of the major vitelline envelope proteins in the plasma and oocytes of the maturing female rainbow trout, Oncorhynchus mykiss." Journal of Endocrinology 135, no. 2 (1992): 303—NP. http://dx.doi.org/10.1677/joe.0.1350303.

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ABSTRACT The major vitelline envelope proteins were detected in the plasma of female rainbow trout maturing under natural conditions by using the Western blot technique. Females were sampled every month from July until ovulation in January. The amount of vitelline envelope proteins in plasma increased markedly as the gonads increased in size from 0·4 to about 15% of the total body weight. The plasma level of oestradiol-17β largely followed the alterations in the amount of vitelline envelope proteins, indicating the endocrine control of vitelline envelope protein synthesis. In addition, plasma

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Granzow, Harald, Barbara G. Klupp, and Thomas C. Mettenleiter. "The Pseudorabies Virus US3 Protein Is a Component of Primary and of Mature Virions." Journal of Virology 78, no. 3 (2004): 1314–23. http://dx.doi.org/10.1128/jvi.78.3.1314-1323.2004.

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ABSTRACT Herpesviruses acquire a primary envelope by budding of capsids at the inner leaflet of the nuclear membrane. They then traverse into the cytoplasm after fusion of the primary envelope with the outer leaflet of the nuclear membrane. In the alphaherpesvirus pseudorabies virus (PrV), the latter process is impaired when the US3 protein is absent. Acquisition of final tegument and envelope occurs in the cytoplasm. Besides the capsid components, only the UL31 and UL34 gene products of PrV have unequivocally been shown to be part of primary enveloped virions, whereas they lack several tegume

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Hu, Dingwen, Haimei Zou, Weijie Chen, et al. "ZDHHC11 Suppresses Zika Virus Infections by Palmitoylating the Envelope Protein." Viruses 15, no. 1 (2023): 144. http://dx.doi.org/10.3390/v15010144.

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Zika virus (ZIKV) is an RNA-enveloped virus that belongs to the Flavivirus genus, and ZIKV infections potentially induce severe neurodegenerative diseases and impair male fertility. Palmitoylation is an important post-translational modification of proteins that is mediated by a series of DHHC-palmitoyl transferases, which are implicated in various biological processes and viral infections. However, it remains to be investigated whether palmitoylation regulates ZIKV infections. In this study, we initially observed that the inhibition of palmitoylation by 2-bromopalmitate (2-BP) enhanced ZIKV in

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Courvalin, J. C., K. Lassoued, H. J. Worman, and G. Blobel. "Identification and characterization of autoantibodies against the nuclear envelope lamin B receptor from patients with primary biliary cirrhosis." Journal of Experimental Medicine 172, no. 3 (1990): 961–67. http://dx.doi.org/10.1084/jem.172.3.961.

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We have identified autoantibodies from two patients with primary biliary cirrhosis (PBC) that recognize the nuclear envelope of mammalian cells on indirect immunofluorescence microscopy. These antibodies bind to a 58-kD integral membrane protein (p58) of the turkey erythrocyte nuclear envelope, which has been previously identified as a membrane receptor for lamin B (Worman, H. J., J. Yuan, G. Blobel, and S. D. Georgatos. 1988. Proc. Natl. Acad. Sci. USA. 85:8531). The antibodies also bind to a 61-kD integral membrane protein (p61) of the rat liver nuclear envelope. Affinity-purified antibodies

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Frank, John A., Manvendra Singh, Harrison B. Cullen, et al. "Evolution and antiviral activity of a human protein of retroviral origin." Science 378, no. 6618 (2022): 422–28. http://dx.doi.org/10.1126/science.abq7871.

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Endogenous retroviruses are abundant components of mammalian genomes descended from ancient germline infections. In several mammals, the envelope proteins encoded by these elements protect against exogenous viruses, but this activity has not been documented with endogenously expressed envelopes in humans. We report that the human genome harbors a large pool of envelope-derived sequences with the potential to restrict retroviral infection. To test this, we characterized an envelope-derived protein, Suppressyn. We found that Suppressyn is expressed in human preimplantation embryos and developing

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Dissertations / Theses on the topic "Envelope protein"

1

He, Runtao. "Characterization of dengue virus envelope protein." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24745.pdf.

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Vijayaraghavan, Balaje. "Identification and characterization of protein-protein interactions in the nuclear envelope." Doctoral thesis, Stockholms universitet, Institutionen för neurokemi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-148432.

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The nuclear envelope forms the interface between the nucleus and the cytoplasm. The nuclear envelope consists of the two concentric lipid membranes, the nuclear pores and the nuclear lamina. The inner nuclear membrane contains hundreds of unique transmembrane proteins showing high tissue diversity. Mutations of some proteins in the nuclear envelope give rise to a broad spectrum of diseases called envelopathies or laminopathies. In this thesis, I aimed to study the functional organization of the nuclear envelope by identifying and characterizing interactions between the nuclear envelope protein

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Vojta, Aleksandar. "Protein Translocon at the Outer Envelope of Chloroplasts." Diss., lmu, 2006. http://nbn-resolving.de/urn:nbn:de:bvb:19-62766.

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Xu, Xiaodong. "Expression and characterization of HIV-1 envelope protein." Thesis, University of Reading, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500515.

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We postulated that gp120 and CD4 interaction might expose cryptic epitopes on gp120 that could be immunogenic and so widen the immunogenic response. A fusion protein of a C clade strain of HIV-1 (HIVCN54) gp120 and full-length human CD4 was constructed and expressed using a recombinant baculovirus system. The protein was purified, characterized and used to immunize rabbits. The antiserum generated had an expected anti-gp120 activity and demonstrated a higher capacity than a control serum raised to gp120 alone to block b12 binding, a marker of neutralization. A formal neutralization assay howev

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Reading, Steven A. "Antibodies to the envelope protein of HIV-1." Thesis, University of Warwick, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.396875.

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Serafin, Ina Loretta. "Epitope mapping of the dengue 3 envelope protein." Thesis, Queensland University of Technology, 1999.

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Bouchnak, Imen. "Biogénèse du chloroplaste : Voies d'import alternatives." Thesis, Université Grenoble Alpes (ComUE), 2018. http://www.theses.fr/2018GREAV074.

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Le chloroplaste est un composant majeur de la cellule végétale. Cet organite est le fruit d’une endosymbiose, survenue entre une cellule eucaryote et une cyanobactérie. Ainsi, 95% des gènes codant pour les protéines plastidiales ont été transférés vers le génome nucléaire au cours de l’évolution. En conséquence, la plupart des protéines chloroplastiques sont aujourd’hui codées par le noyau, synthétisées dans le cytosol sous forme de précurseurs dotés d’une une extension N-terminale clivable (le "peptide de transit") et ensuite importées sans les chloroplastes via le système TOC/TIC (Translocon

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Jaffer, Ali Mohammed Hakim. "Multifaceted roles of the transmembrane nuclear envelope protein, Samp1." Doctoral thesis, Stockholms universitet, Institutionen för neurokemi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-141816.

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The eukaryotic nuclear envelope (NE), separates the nucleoplasm from cytoplasm and is made up of two concentric lipid membranes, the outer and the inner nuclear membranes (ONM and INM), the nuclear pore complexes (NPCs) and an underlying filamentous nuclear lamina. The INM contains hundreds of unique transmembrane proteins of which only a handful have been characterized. In this thesis, I aimed to understand the functional organization of proteins in the nuclear envelope and I focused on investigating the functions of a recently identified INM transmembrane protein, Samp1. We have developed a

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Firlej-Kwoka, Ewa. "Protein import into the inner envelope membrane of chloroplasts." Diss., kostenfrei, 2008. http://edoc.ub.uni-muenchen.de/8694/.

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Samson, Camille. "Une représentation en trois dimensions de l'interface entre l'enveloppe nucléaire et la chromatine." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS048/document.

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Le noyau est un organite caractéristique des cellules eucaryotes et les propriétés mécaniques de ce dernier jouent un rôle essentiel dans le comportement de la cellule, notamment sa motilité, sa polarité et sa survie. Le noyau est entouré par une enveloppe comprenant une membrane interne et une membrane externe, ainsi que de nombreuses protéines. Mes objectifs de thèse étaient de comprendre des mécanismes moléculaires déficients dans deux types de maladies génétiques causées par des mutations dans les lamines: la dystrophie musculaire d’Emery-Dreifuss et les syndromes de type progéroïde.Dans u

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Books on the topic "Envelope protein"

1

Thick, Jane Anne. A novel nuclear envelope protein. University of Birmingham, 1989.

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2

Hyllner, Sven Johan. Vitelline envelope proteins in teleost fish. Dept. of Zoophysiology, University of Göteborg, 1994.

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3

Begbie, Guy. Destruction in art reconstruction: [bombed envelope book]. [publisher not identified], 2011.

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4

Joe, Bentz, ed. Viral fusion mechanisms. CRC Press, 1993.

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Apatoff, Brian Richard. Trophic effects of neuroleukin on central neurons and antagonism by HIV envelope protein. 1988.

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Bayley, Cynthia Anne. Analysis of a region of the human immunodeficiency virus envelope protein important for infectivity. 1990.

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Corty, Robert. Studies on the oligomerization of the dengue envelope protein and their implications for the fusion pathway. 2010.

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8

Gombart, Adrian F. OpMNPV p32, a baculovirus polyhedral envelope-associated protein: Genetic location, nucleotide sequence, transcriptional mapping and immunocytochemical characterization. 1989.

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9

Tien, Roselyn Fatt Ing. Insertion characteristics of chloroplast outer envelope proteins. 1999.

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Membrane Trafficking in Viral Replication (Current Topics in Microbiology and Immunology). Springer, 2004.

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Book chapters on the topic "Envelope protein"

1

Zhang, Jiapu. "Envelope Protein (E-Protein)." In Springer Series in Biophysics. Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-36773-1_20.

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Jain, Madhulika, Eric D. Chow, and Jeffery S. Cox. "The MmpL Protein Family." In The Mycobacterial Cell Envelope. ASM Press, 2014. http://dx.doi.org/10.1128/9781555815783.ch12.

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Delogu, Giovanni, Fabiana Bigi, Seyed E. Hasnain, and Angel Cataldi. "Enigmatic Proteins from the Surface: the Erp, PE, and PPE Protein Families." In The Mycobacterial Cell Envelope. ASM Press, 2014. http://dx.doi.org/10.1128/9781555815783.ch8.

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Collas, Philippe. "Sea Urchin Nuclear Envelope Assembly In Vitro." In Lipid and Protein Traffic. Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-51463-0_22.

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Schneewind, Olaf, and Dominique M. Missiakas. "Staphylococcal Protein Secretion and Envelope Assembly." In Gram-Positive Pathogens. ASM Press, 2019. http://dx.doi.org/10.1128/9781683670131.ch37.

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Graumann, Katja, and David E. Evans. "The Nuclear Envelope - Structure and Protein Interactions." In Annual Plant Reviews. John Wiley & Sons Ltd, 2013. http://dx.doi.org/10.1002/9781118472507.ch2.

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Hong, M. L., M. G. S. Yap, B. T. Koh, and L. Chan. "Production of Recombinant HTLV-I Envelope Protein." In Biochemical Engineering for 2001. Springer Japan, 1992. http://dx.doi.org/10.1007/978-4-431-68180-9_36.

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Dai, Lianpan, Qihui Wang, Hao Song, and George Fu Gao. "Zika Virus Envelope Protein and Antibody Complexes." In Subcellular Biochemistry. Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8456-0_7.

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de Boer, Douwe, Johan Hageman, Rien Pilon, Twan America, and Peter Weisbeek. "Protein Translocation across the Chloroplast Envelope Membrane." In Plant Molecular Biology 2. Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3304-7_58.

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Kim, Dae In, Samuel C. Jensen, and Kyle J. Roux. "Identifying Protein-Protein Associations at the Nuclear Envelope with BioID." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3530-7_8.

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Conference papers on the topic "Envelope protein"

1

Jena, Sambedika, Pushpendra Kumar, and Dheerendra Mishra. "Tricyclic peptide of RP 71955 exhibits anti-dengue activity against DENV-2 envelope 3 protein: an in silico and HPC-driven MD simulation analysis." In 2024 OPJU International Technology Conference (OTCON) on Smart Computing for Innovation and Advancement in Industry 4.0. IEEE, 2024. http://dx.doi.org/10.1109/otcon60325.2024.10687381.

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Bartling, Craig, Angela Minard-Smith, Kate H. Kucharzyk, Jennifer Busch-Harris, and Larry Mullins. "Interpreting Omic Data for Microbially Influenced Corrosion: Lessons from a Case Study Involving a Seawater Injection System." In CORROSION 2017. NACE International, 2017. https://doi.org/10.5006/c2017-09445.

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Abstract Microbiologically influenced corrosion (MIC) is a significant source of pitting corrosion affecting oil and gas pipelines, wells, and a variety of surface facilities. Understanding of MIC is greatly enhanced through DNA and protein sequencing technologies. This paper highlights the need to understand the methods used to generate the data, the data quality, and the limitations associated with data interpretation through a case study involving the metagenomics and proteomic analysis of pig envelope debris and seawater samples from various locations within a seawater injection system sus

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Palacios, Jose, Joseph Szefi, Douglas Wolfe, and Matthew Bailey. "Ice Testing of a Centrifugally Powered Pneumatic Deicing System for Helicopter Rotor Blades." In Vertical Flight Society 70th Annual Forum & Technology Display. The Vertical Flight Society, 2014. http://dx.doi.org/10.4050/f-0070-2014-9662.

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A novel pneumatic approach to protect helicopter rotor blades from ice accretion is presented in this paper. The system relies on centrifugally generated pressures to deform a 0.508 mm (0.02 in.) thick titanium leading edge cap. The leading edge cap is protected by a 10 μm (390 microinch) thick Ti-Al-N erosion resistant coating. Beneath the titanium leading edge, six (6) pneumatic diaphragms were installed. The diaphragms are normally deflated under vacuum against the surface of the blade, and are inflated when ice accretion thickness reaches a critical value. The deformation of the leading ed

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"Structure and dynamics of the SARS-CoV-2 envelope protein." In Bioinformatics of Genome Regulation and Structure/Systems Biology (BGRS/SB-2022) :. Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 2022. http://dx.doi.org/10.18699/sbb-2022-167.

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Krasilnikova, A. A., M. S. Dodina, and L. I. Kozlovskaya. "DEVELOPMENT OF A REPRODUCIBLE SYSTEM FOR OBTAINING SOLUBLE PROTEIN E OF TICK-BORNE ENCEPHALITIS VIRUS." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-191.

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Envelope protein E of tick-borne encephalitis virus was obtained by coexpression with chaperones in E. coli in an aggregated and partially soluble forms. Conditions were optimized for protein purification in order to increase protein yield. According to ELISA’s results, protein interacted with antibodies, hence it was in its native conformation.

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Sharma, G., M. Badescu, A. Dubey, et al. "Kinematics and Workspace Analysis of Protein Based Nano-Motors." In ASME 2004 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2004. http://dx.doi.org/10.1115/detc2004-57569.

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Kinematic and workspace analyses are performed to predict the performance of a new nanoscale biomolecular motor: The Viral Protein Linear (VPL) Motor. The motor is based on a conformational change observed in a family of viral envelope proteins when subjected to a changing pH environment. The conformational change produces a motion of about 10 nm, making the VPL a basic linear actuator, which can be further interfaced with other organic/inorganic nanoscale components such as DNA actuators and carbon nanotubes. This paper presents the principle of operation of the VPL motor and the development

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Pulido, D., M. Torrent, M. V. Nogués, and E. Boix. "The role of Gram-negative envelope LPS on the bactericidal properties of proteins and peptides: the case of eosinophil cationic protein." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0008.

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Gorav, Gorav, Vrushali Khedekar, Geetha K. Varier, and P. Nandakumar. "Enhanced Uptake and Retention of Graphene Quantum Dots in HeLa Cell Nuclei." In Bio-Optics: Design and Application. Optica Publishing Group, 2023. http://dx.doi.org/10.1364/boda.2023.jtu4a.6.

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The nuclear pore complex (NPC) is an intricate protein structure located on the nuclear envelope that functions as the sole gateway for nuclear cytoplasm transport. Our experimental studies show the dual uptake rates of GQDs in HeLa nuclei indicate two phenomena occurring simultaneously. Export studies confirm bidirectionality and retention.

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Guimarães, Rosane, Andrea Silva, Luciane Gaspar, et al. "Immunization with recombinant, plant-produced yellow fever virus envelope (E) protein vaccine candidates in rhesus macaques." In I Seminário Anual Científico e Tecnológico em Imunobiológicos. Instituto de Tecnologia em Imunobiológicos, 2013. http://dx.doi.org/10.35259/isi.sact.2013_27301.

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Otsuka, S., Y. Hirano, H. Takahashi, M. Kumeta, K. Takeyasu, and S. H. Yoshimura. "Single-molecule imaging, force measurement and fluorescence observation reveal protein and chromosome dynamics around the nuclear envelope." In 2007 International Symposium on Micro-NanoMechatronics and Human Science. IEEE, 2007. http://dx.doi.org/10.1109/mhs.2007.4420872.

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Reports on the topic "Envelope protein"

1

Chen, Junping, Zach Adam, and Arie Admon. The Role of FtsH11 Protease in Chloroplast Biogenesis and Maintenance at Elevated Temperatures in Model and Crop Plants. United States Department of Agriculture, 2013. http://dx.doi.org/10.32747/2013.7699845.bard.

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specific objectives of this proposal were to: 1) determine the location, topology, and oligomerization of FtsH11 protease; 2) identify the substrate/s of FtsH11 and the downstream components involved in maintaining thermostability of chloroplasts; 3) identify new elements involved in FtsH11 protease regulatory network related to HT adaptation processes in chloroplast; 4) Study the role of FtsH11 homologs from crop species in HT tolerance. Background to the topic: HT-tolerant varieties that maintain high photosynthetic efficiency at HT, and cope better with daily and seasonal temperature fluctu

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Bradford, Kent, Haim Nerson, Gregory Wellbaum, and Menahem Edelstein. Environmental, Developmental and Physiological Determinants of Curcurbit Seed Quality. United States Department of Agriculture, 1998. http://dx.doi.org/10.32747/1998.7695837.bard.

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Environmental, developmental, physiological and biochemical determinants of cucurbit seed quality were investigated in field and laboratory experiments. The major factor influencing seed quality is seed maturity at harvest, with both immature and overmature seeds exhibiting reduced quality. Planting density and fruit load per plant can be manipulated to maximize seed yield per unit area without adversely affecting seed quality. Seeds harvested at optimal maturity will have the greatest germination vigor and will maintain quality longer during storage. Seed priming can improve germination rates

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Gelb, Jr., Jack, Yoram Weisman, Brian Ladman, and Rosie Meir. Identification of Avian Infectious Brochitis Virus Variant Serotypes and Subtypes by PCR Product Cycle Sequencing for the Rational Selection of Effective Vaccines. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586470.bard.

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Objectives 1. Determine the serotypic identities of 40 recent IBV isolates from commercial chickens raised in the USA and Israel. 2. Sequence all IBV field isolates using PCR product cycle sequencing and analyze their S 1 sequence to detennine their homology to other strains in the Genbank and EMBL databases. 3. Select vaccinal strains with the highest S 1 sequence homology to the field isolates and perform challenge of immunity studies in chickens in laboratory trials to detennine level of protection afforded by the vaccines. Background Infectious bronchitis (IB) is a common, economically imp

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