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Dissertations / Theses on the topic 'Envelope protein'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

He, Runtao. "Characterization of dengue virus envelope protein." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24745.pdf.

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2

Vijayaraghavan, Balaje. "Identification and characterization of protein-protein interactions in the nuclear envelope." Doctoral thesis, Stockholms universitet, Institutionen för neurokemi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-148432.

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The nuclear envelope forms the interface between the nucleus and the cytoplasm. The nuclear envelope consists of the two concentric lipid membranes, the nuclear pores and the nuclear lamina. The inner nuclear membrane contains hundreds of unique transmembrane proteins showing high tissue diversity. Mutations of some proteins in the nuclear envelope give rise to a broad spectrum of diseases called envelopathies or laminopathies. In this thesis, I aimed to study the functional organization of the nuclear envelope by identifying and characterizing interactions between the nuclear envelope protein

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3

Vojta, Aleksandar. "Protein Translocon at the Outer Envelope of Chloroplasts." Diss., lmu, 2006. http://nbn-resolving.de/urn:nbn:de:bvb:19-62766.

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4

Xu, Xiaodong. "Expression and characterization of HIV-1 envelope protein." Thesis, University of Reading, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.500515.

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We postulated that gp120 and CD4 interaction might expose cryptic epitopes on gp120 that could be immunogenic and so widen the immunogenic response. A fusion protein of a C clade strain of HIV-1 (HIVCN54) gp120 and full-length human CD4 was constructed and expressed using a recombinant baculovirus system. The protein was purified, characterized and used to immunize rabbits. The antiserum generated had an expected anti-gp120 activity and demonstrated a higher capacity than a control serum raised to gp120 alone to block b12 binding, a marker of neutralization. A formal neutralization assay howev

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5

Reading, Steven A. "Antibodies to the envelope protein of HIV-1." Thesis, University of Warwick, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.396875.

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6

Serafin, Ina Loretta. "Epitope mapping of the dengue 3 envelope protein." Thesis, Queensland University of Technology, 1999.

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7

Bouchnak, Imen. "Biogénèse du chloroplaste : Voies d'import alternatives." Thesis, Université Grenoble Alpes (ComUE), 2018. http://www.theses.fr/2018GREAV074.

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Le chloroplaste est un composant majeur de la cellule végétale. Cet organite est le fruit d’une endosymbiose, survenue entre une cellule eucaryote et une cyanobactérie. Ainsi, 95% des gènes codant pour les protéines plastidiales ont été transférés vers le génome nucléaire au cours de l’évolution. En conséquence, la plupart des protéines chloroplastiques sont aujourd’hui codées par le noyau, synthétisées dans le cytosol sous forme de précurseurs dotés d’une une extension N-terminale clivable (le "peptide de transit") et ensuite importées sans les chloroplastes via le système TOC/TIC (Translocon

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8

Jaffer, Ali Mohammed Hakim. "Multifaceted roles of the transmembrane nuclear envelope protein, Samp1." Doctoral thesis, Stockholms universitet, Institutionen för neurokemi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-141816.

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The eukaryotic nuclear envelope (NE), separates the nucleoplasm from cytoplasm and is made up of two concentric lipid membranes, the outer and the inner nuclear membranes (ONM and INM), the nuclear pore complexes (NPCs) and an underlying filamentous nuclear lamina. The INM contains hundreds of unique transmembrane proteins of which only a handful have been characterized. In this thesis, I aimed to understand the functional organization of proteins in the nuclear envelope and I focused on investigating the functions of a recently identified INM transmembrane protein, Samp1. We have developed a

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9

Firlej-Kwoka, Ewa. "Protein import into the inner envelope membrane of chloroplasts." Diss., kostenfrei, 2008. http://edoc.ub.uni-muenchen.de/8694/.

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10

Samson, Camille. "Une représentation en trois dimensions de l'interface entre l'enveloppe nucléaire et la chromatine." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS048/document.

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Le noyau est un organite caractéristique des cellules eucaryotes et les propriétés mécaniques de ce dernier jouent un rôle essentiel dans le comportement de la cellule, notamment sa motilité, sa polarité et sa survie. Le noyau est entouré par une enveloppe comprenant une membrane interne et une membrane externe, ainsi que de nombreuses protéines. Mes objectifs de thèse étaient de comprendre des mécanismes moléculaires déficients dans deux types de maladies génétiques causées par des mutations dans les lamines: la dystrophie musculaire d’Emery-Dreifuss et les syndromes de type progéroïde.Dans u

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11

Blagden, Sarah Patricia. "Meteor, a novel Drosophila nuclear envelope-associated protein involved in mitosis." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.590616.

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12

Chand, Puran. "Molecular and immunological characterisation of a major envelope protein of capripoxvirus." Thesis, University of Surrey, 1992. http://epubs.surrey.ac.uk/2774/.

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Analysis of the proteins of capripoxvirus (KS-1) revealed a 32kd protein that is one of the major structural proteins of the virus and is localised in the virus envelope. Monospecific serum prepared against the 32kd envelope protein neutralised the virus indicating that this protein contains neutralising epitopes. Lymphocyte proliferation studies, using the 32kd protein and peripheral blood mononuclear cells from capripoxvirus (KS-i) vaccinated sheep, showed that this protein strongly induced cellmediated immune responses. The 32kd protein is capripoxvirus specific and induced antibodies in ea

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13

Kuroda, Shun'ichi. "STUDIES ON HEPATITIS B VIRUS ENVELOPE PROTEIN CONTAINING PRE-S PEPTIDE." Kyoto University, 1992. http://hdl.handle.net/2433/168904.

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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものである<br>Kyoto University (京都大学)<br>0048<br>新制・論文博士<br>博士(農学)<br>乙第8019号<br>論農博第1796号<br>新制||農||640(附属図書館)<br>学位論文||H4||N2518(農学部図書室)<br>UT51-92-U255<br>(主査)教授左右田健次, 教授駒野徹, 教授清水昌<br>学位規則第4条第2項該当

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14

Ita, Sergio. "Adaptive Evolution of the SIV Envelope Protein During Early SIV Infection." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493399.

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Primate lentiviruses (PLVs), including human immunodeficiency virus type 1 (HIV-1), HIV type 2 (HIV-2), and the simian immunodeficiency viruses (SIVs), cause persistent lifelong infections despite the presence of virus-specific adaptive immune responses. The target of antibodies is the viral envelope glycoprotein (Env), which is expressed on the surface of virions and infected cells as a trimer of gp120:gp41 heterodimers. SIV env sequence variation arising from evasion of antibodies is well established and closely mimics the pattern observed in HIV-infected human patients, yet despite the exp

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15

Thomas, Joanne Marie. "Assembly of influenza viruses." Thesis, University of Reading, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326757.

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16

Studte, Carsten. "Characterization of the membrane protein Prat1 in the inner envelope of chloroplasts." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-149042.

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17

Mudumbi, Krishna Chaitanya. "NUCLEAR ENVELOPE TRANSMEMBRANE PROTEIN DISTRIBUTION AND TRANSPORT STUDIED BY SINGLE-MOLECULE MICROSCOPY." Diss., Temple University Libraries, 2018. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/489984.

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Biology<br>Ph.D.<br>The nucleus of eukaryotic cells is a vitally important organelle that sequesters the genetic information of the cell, and protects it with the help of two highly evolved structures, the nuclear envelope (NE) and nuclear pore complexes (NPCs). Together, these two structures mediate the bidirectional trafficking of molecules between the nucleus and cytoplasm by forming a barrier. NE transmembrane proteins (NETs) embedded in either the outer nuclear membrane (ONM) or the inner nuclear membrane (INM) play crucial roles in both nuclear structure and functions, including: genome

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18

Batzenschlager, Morgane. "Diversité fonctionnelle des protéines GIPs/MZT1 (Gamma-tubulin complex protein 3- Interacting Proteins/Mitotic spindle organiZing proTein1) à l'interface nucléo-cytoplasmique chez Arabidopsis thaliana." Thesis, Strasbourg, 2014. http://www.theses.fr/2014STRAJ030.

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Chez Arabidopsis, l’enveloppe nucléaire constitue un site de nucléation des microtubules à partir des complexes à gamma-tubuline. Conservées des plantes à l'Homme, les protéines GIPs/MZT1 ont été initialement découvertes comme partenaires d’AtGCP3. J’ai consacré ma thèse à la caractérisation moléculaire et fonctionnelle des AtGIPs et de leurs partenaires à l’interface nucléocytoplasmique. Mes résultats confirment l’appartenance des GIPs aux complexes à gamma-tubuline, et démontrent leur association entre elles et avec TSA1 (TonSoKu [TSK]-Associating protein 1) et l'histone centromérique CenH3.

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19

Patel, Shalaka S. "Functional analysis of the nuclear envelope associated protein MAF1 and preliminary investigation of the coiled-coil protein TMD1." Connect to this title online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1110300984.

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Thesis (Ph. D.)--Ohio State University, 2005.<br>Document formatted into pages. Includes bibliographical references. Abstract available online via OhioLINK's ETD Center; full text release delayed at author's request until 2006 March 9.

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20

Patel, Janisha. "An investigation of the complexes formed between the hepatitis C virus E1 and E2 glycoproteins." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342004.

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21

Chojnacki, Jakub. "Envelope protein domains of duck hepatitis B virus : role in assembly and infectivity /." Connect to thesis, 2005. http://eprints.unimelb.edu.au/archive/00001738.

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Thesis (Ph.D.)--University of Melbourne, Dept. of Microbiology and Immunology, The Macfarlane Burnet Institute for Medical Research and Public Health, 2006.<br>Typescript. Includes bibliographical references (leaves 155-177).

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22

Sabbatini, G. P., and G. P. Sabbatini. "Rat liver nuclear envelope insulin binding and its effects on endogenous protein kinases." Doctoral thesis, University of Cape Town, 1992. http://hdl.handle.net/11427/23361.

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The postulated model for the insulin - stimulated induction of mRNA efflux (Purrello et al., 1983) is based on the demonstrated binding of insulin to intracellular membrane structures (see chapter 2, section 2.2.1), and the in vitro effect of insulin on nuclear envelope phosphorylation, NTPase activity, and mRNA efflux (see chapter 5, section 5.1). These independent observations have led to the development of a model for the direct induction by insulin, at the level of the nucleus, of mRNA efflux (figure 1.1). However, the specific intracellular insulin binding has been inf erred from kinetic

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23

Zhao, Qiao. "Characterization of The Arabidopsis Nuclear Import Factor of Ran and Functional Analysis of Nuclear Envelope Associate Protein Wit." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1210266926.

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24

Clark, Margaret Jean. "Exploring Dengue Virus Entry through Small Molecule Inhibition and Mutagenesis of the Envelope Protein." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10703.

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Over one-third of the world’s population is at risk for infection with dengue virus (DENV), a mosquito-borne virus that can cause a severe febrile disease. There are no specific treatments available for dengue infection, and much remains unknown about how DENV interaction with the host cell leads to a successful infection. This dissertation examines DENV entry using small molecule inhibitors and mutagenesis of the envelope (E) protein, the major protein on the viral surface. This work grew from our initial observation that small molecule GNF-2 is capable of lowering DENV yield when present at

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25

Sjuts, Inga [Verfasser], and Jürgen [Akademischer Betreuer] Soll. "Topology and function of the inner envelope protein Tic110 / Inga Sjuts ; Betreuer: Jürgen Soll." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/1159879796/34.

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26

BERTORA, STEFANIA. "ROLE OF NUCLEAR ENVELOPE PROTEIN MAN1 IN NUCLEAR ORGANISATION AND MAINTENANCE OF GENOME STABILITY." Doctoral thesis, Università degli Studi di Milano, 2018. http://hdl.handle.net/2434/554706.

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The eukaryotic cell nucleus is characterized by a defined spatial organization of the chromatin, which relies on the physical tethering of many genomic loci to the inner surface of the nuclear envelope. This interaction is mainly mediated by lamins and lamin-associated proteins, which create a protein network at the nuclear periphery called nuclear lamina. Man1 is a member of a lamin-associated protein family known as LEM-domain proteins, which are characterized by the presence of a highly conserved domain, called LEM, that mediates the interaction with the chromatin. Data obtained with the ye

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27

Krzyzaniak, Magdalena Anna. "Role of the gM/gN glycoprotein complex in the final assembly and egress of the human cytomegalovirus (HCMV)." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2008. https://www.mhsl.uab.edu/dt/2008p/krzyzaniak.pdf.

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28

Chen, Yuxin. "Characterization of Envelope-Specific Antibody Response Elicited by HIV-1 Vaccines: A Dissertation." eScholarship@UMMS, 2015. https://escholarship.umassmed.edu/gsbs_diss/760.

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Despite 30 years of intensive research，an effective human immunodeficiency virus (HIV) vaccine still remains elusive. The desirable immune response capable of providing protection against HIV acquisition is still not clear. The accumulating evidence learned from a recent vaccine efficacy correlate study not only confirmed the importance of antibody responses, but also highlighted potential protective functions of antibodies with a broad repertoire of HIV-1 epitope specificities and a wide range of different antiviral mechanisms. This necessitates a deep understanding of the complexity and dive

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29

Chen, Yuxin. "Characterization of Envelope-Specific Antibody Response Elicited by HIV-1 Vaccines: A Dissertation." eScholarship@UMMS, 2001. http://escholarship.umassmed.edu/gsbs_diss/760.

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Despite 30 years of intensive research，an effective human immunodeficiency virus (HIV) vaccine still remains elusive. The desirable immune response capable of providing protection against HIV acquisition is still not clear. The accumulating evidence learned from a recent vaccine efficacy correlate study not only confirmed the importance of antibody responses, but also highlighted potential protective functions of antibodies with a broad repertoire of HIV-1 epitope specificities and a wide range of different antiviral mechanisms. This necessitates a deep understanding of the complexity and dive

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30

Chan, Wing-lim, and 陳穎廉. "The SARS coronavirus envelope protein E targets the PALS1 tight junction factor and alters formation of tight junctions of epithelialcells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B47169242.

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Tight junctions, as zones of close contact between epithelial and endothelial cells, form a physical barrier as one of the first host defense strategies that prevent the intrusion of pathogens across epithelia and endothelia. Recently, an interaction between the Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) envelope protein (E) and PALS1, a member of the CRB tight junction complex, was identified in the Virus-Host Interaction group at HKU-Pasteur Research Centre (Teoh et al, 2010). In this report, I present in vitro data which helps to better understand how this protein-protein int

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31

Barton, Maria. "Evaluating XMRV As An Indicator Of Prostate Cancer Risk." Kent State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=kent1309619878.

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32

Teoh, Kim Tat. "The E envelope protein of the SARS coronavirus interacts with the pals1 tight junction protein through its PDZ domain consequences for polarity of infected epithelial cells /." Click to view the E-thesis via HKUTO, 2010. http://sunzi.lib.hku.hk/hkuto/record/B43913210.

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33

Williamson, Shawn T. "Trafficking of integral membrane proteins of the inner nuclear membrane can be mediated by the ''sorting motif'' of autographa californica nucleopolyhedrovirus odv-e66." Texas A&M University, 2005. http://hdl.handle.net/1969.1/4353.

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The amino-terminal 33 amino acids of the baculovirus integral membrane protein, ODV-E66, are sufficient for localization of fusion proteins to viralinduced intranuclear microvesicles (MV) and occlusion derived virus envelopes during infection, and has been termed the sorting motif (SM). When abundantly expressed, SM-fusions are also detected in the inner nuclear membrane (INM), outer nuclear membrane and endoplasmic reticulum of infected cells, suggesting proteins with the SM use the same trafficking pathway as cellular INM proteins to traffic to nuclear membranes. This study identifies the es

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34

Howard, Christopher Bruce. "Identification of epitopes on the Dengue virus type 4 envelope glycoprotein involved in neutralisation by antibodies." Thesis, Queensland University of Technology, 2006. https://eprints.qut.edu.au/16401/1/Christopher_Howard_Thesis.pdf.

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Dengue virus (DENV) is the causative agent of dengue fever (DF), the most prevalent arthropod-borne viral disease in the world and therefore is considered an emerging global health threat. The four DENV serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) that infect humans are distinguished from one another by unique antigenic determinants (epitopes) on the DENV envelope (E) protein. The E protein is the primary antigenic site of the DENV and is responsible for inducing neutralising antibody (Ab) and cell mediated immune response in DENV infected hosts. The DENV E protein also mediates attachment

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35

Howard, Christopher Bruce. "Identification of epitopes on the Dengue virus type 4 envelope glycoprotein involved in neutralisation by antibodies." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16401/.

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Dengue virus (DENV) is the causative agent of dengue fever (DF), the most prevalent arthropod-borne viral disease in the world and therefore is considered an emerging global health threat. The four DENV serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) that infect humans are distinguished from one another by unique antigenic determinants (epitopes) on the DENV envelope (E) protein. The E protein is the primary antigenic site of the DENV and is responsible for inducing neutralising antibody (Ab) and cell mediated immune response in DENV infected hosts. The DENV E protein also mediates attachment

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36

Ainsworth, Julia. "Comparison of p53 and MAGI-3 regulation mediated by the E6 protein from high-risk human papillomavirus types 18 and 33." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=112368.

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The HPV E6-p53 interaction is well-understood, but not for all high-risk HPV types. In addition, HPV E6 p53-independent functions are gaining recognition for their importance in cellular transformation but require clarification. Thus, the aim of this study was two-fold: (1) to gain insight into the p53-E6 interaction for high-risk HPV-33 and, (2) to explore how high-risk HPV E6 proteins targets cellular MAGI-3 for degradation.<br>In vivo and in vitro results indicated that E6 from HPV types 18 and 33 interacted similarly with p53 although, variants of the HPV-33 E6 prototype demonstrated inter

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37

Teoh, Kim Tat, and 張錦達. "The E envelope protein of the SARS coronavirus interacts with the pals1 tight junction protein through its PDZ domain: consequences for polarity of infected epithelial cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B43913210.

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38

Costa, Karla Dutra. "Análise filogenética do gene da proteína envelope do vírus dengue sorotipo 3 no cenário global." Universidade Federal de Goiás, 2018. http://repositorio.bc.ufg.br/tede/handle/tede/8513.

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Submitted by Liliane Ferreira (ljuvencia30@gmail.com) on 2018-05-25T14:03:28Z No. of bitstreams: 2 Dissertação - Karla Dutra Costa - 2018.pdf: 3075185 bytes, checksum: 4457eb761c72d55aa56e0fce73443cc1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)<br>Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-05-28T11:14:44Z (GMT) No. of bitstreams: 2 Dissertação - Karla Dutra Costa - 2018.pdf: 3075185 bytes, checksum: 4457eb761c72d55aa56e0fce73443cc1 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)<br>Made availabl

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Qbadou, Soumya. "Function and Topology of Toc64,a subunit of the protein translocation machinery of the chloroplast outer envelope." Diss., [S.l.] : [s.n.], 2006. http://edoc.ub.uni-muenchen.de/archive/00005867.

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40

Wagner, Heike M. "Competitive ELISAs confirm that equine arteritis virus-infected horses develop antibodies to the M viral envelope protein." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973408219.

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Cox, Charles John. "Persistent EBV infection in the common marmoset : the effect of immunization with envelope glyco protein gp 340." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266318.

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Studte, Carsten [Verfasser], and Jürgen [Akademischer Betreuer] Soll. "Characterization of the membrane protein Prat1 in the inner envelope of chloroplasts / Carsten Studte. Betreuer: Jürgen Soll." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2012. http://d-nb.info/1027066291/34.

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43

Matanin, Brad Matthew. "Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/33194.

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Porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of a pandemic that has been devastating the U.S. and global swine industry for more than twenty years. PRRSV vaccine development is challenging due to virus heterogeneity. Evidence indicates that the major envelope protein, GP5, is the primary target for a subunit vaccine. In native virions GP5 primarily exists as a disulfide linked complex with the membrane protein, M, which also possesses immunogenic properties. Recent studies report that the GP5/M complex is a more significant vaccine candidate. Currently, no bulk

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44

Sousa, Jéssica Fabiana Moreira de. "Characterization of the nuclear envelope alterations during ageing." Master's thesis, 2018. http://hdl.handle.net/10773/25139.

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Ageing is characterized by an overall organismal fitness decrease across lifespan. Hence, the risk of developing complex diseases as neuropathologic disorders, diabetes, cancer and cardiovascular diseases increases with age. Despite a huge effort to determine the underlying molecular and cellular changes, the precise molecular mechanisms that regulate the ageing process remain elusive. Although, in the last few years, the protein regulation at the nuclear periphery, particularly of the nuclear lamina and lamina-associated proteins, has been pointed out as a major contributor to the ageing proc

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45

TAN, AN-JIE, and 譚安絜. "Protein Expression and Isolation of the MERS-CoVSpike Protein, Membrane Protein and Envelope Protein." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/ftq948.

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碩士<br>國立臺灣大學<br>生化科技學系<br>107<br>MERS-CoV was first identified in Saudi Arabia in 2012 and has spread to Europe, Asia, Africa and North America. A total of 2,249 MERS-CoV-infected cases with a mortality of 36% was reported in September 2018. WHO declares that there are no vaccines or specific treatment available now. Thus, there is an urgent need for the development of vaccines and theranostic antibodies for better pandemic preparedness. MERS-CoV has a positive sense, single-stranded RNAs, including the spike (S), membrane (M) and envelope (E) genes. These three viral surface structure protein

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46

"Localization, Dynamics and Functions of the Coronavirus Envelope Protein." Doctoral diss., 2012. http://hdl.handle.net/2286/R.I.14824.

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abstract: Coronaviruses are a medically significant group of viruses that cause respiratory and enteric infections in humans and a broad range of animals. Coronaviruses assemble at the internal membranes of the endoplasmic reticulum- Golgi intermediate compartment (ERGIC). While there is a basic understanding of how viruses assemble at these membranes, the full mechanistic details are not understood. The coronavirus envelope (E) protein is a small multifunctional viroporin protein that plays a role in virus assembly but its function is unknown. The two goals of this study were : 1. To identify

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47

Kesavardana, Sannula. "Protein Engineering and Stabilization of HIV-1 Envelope Glycoprotein." Thesis, 2014. http://etd.iisc.ac.in/handle/2005/3493.

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A number of viral diseases such as Hepatitis B, small pox, measles, rubella and polio have effective vaccines to control or eradicate them. HIV-1 is a lentivirus which infects human immune cells and leads to the disease called AIDS (Acquired Immuno Deficiency Syndrome). Despite much effort since the three decades of its discovery, there is no effective vaccine against HIV-1. The envelope glycoprotein of HIV-1 is the most accessible protein on the virion surface and is essential for HIV-1 infection. Thus, this protein is the primary target for HIV-1 vaccine design. However, HIV-1 has acquired

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48

Kesavardana, Sannula. "Protein Engineering and Stabilization of HIV-1 Envelope Glycoprotein." Thesis, 2014. http://etd.iisc.ernet.in/2005/3493.

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Abstract:

A number of viral diseases such as Hepatitis B, small pox, measles, rubella and polio have effective vaccines to control or eradicate them. HIV-1 is a lentivirus which infects human immune cells and leads to the disease called AIDS (Acquired Immuno Deficiency Syndrome). Despite much effort since the three decades of its discovery, there is no effective vaccine against HIV-1. The envelope glycoprotein of HIV-1 is the most accessible protein on the virion surface and is essential for HIV-1 infection. Thus, this protein is the primary target for HIV-1 vaccine design. However, HIV-1 has acquired

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49

Hung, Chiang Po, and 江柏宏. "Localization of Matrix Protein Co-expressed with Envelope Protein during HIV-1 Assembly." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/57984326738553971300.

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碩士<br>國防醫學院<br>生物及解剖學研究所<br>87<br>The assembly of Human Immunodeficiency Virus (HIV) particles occurred at the plasma membrane of infected cells. However, mechanisms of virus assembly and budding are still unclear. The Gag polyproteins are cleaved to yield three major structural proteins arranged in the following order: NH2-membrane-associated matrix protein (MA)-capsid protein (CA)-Nucleocapsid protein (NC)-COOH. Immunoelectron microscopic studies have shown that the HIV P17MA protein (MA), is a component of the envelope-associated icosadeltahedral capsid that lines the inner surface of the v

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50

馮天霖. "The Effect of Premembrance Protein and Nonstructural Protein 1 on Japanese Encephalitis Virus Envelope Protein Expression." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/22194067954342311353.

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碩士<br>國防醫學院<br>微生物暨免疫學研究所<br>85<br>To explore the influence of premembrane protein (prM) and nonstructural protein 1 (NS1) of Japanese encephalitis virus on the maturation and expression of the envelope protein (E), we constructed the plasmid pJMENS1 which carried the genes of prM, E and NS1 proteins and used it together with plasmids pJEs (with E gene), pJME (with prM and E genes) and pJENS1 (with E and NS1 genes) constructed earlier in the laboratory to proceed the study. We expressed these four plasmids separately in Vero cells and the E protein expression within transfected cells were dete

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